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Page 1: UNIVERSITAS AIRLANGGA DIREKTORAT PENDIDIKAN Tim …journal.unair.ac.id/filerPDF/dentjcc17109b1babs.pdf · 2014. 4. 7. · Fakultas Kedokteran Gigi Abstract Background: Biofilm is

UNIVERSITAS AIRLANGGA DIREKTORAT PENDIDIKANTim Pengembangan Jurnal Universitas AirlanggaKampus C Mulyorejo Surabaya

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Page 2: UNIVERSITAS AIRLANGGA DIREKTORAT PENDIDIKAN Tim …journal.unair.ac.id/filerPDF/dentjcc17109b1babs.pdf · 2014. 4. 7. · Fakultas Kedokteran Gigi Abstract Background: Biofilm is

UNIVERSITAS AIRLANGGA DIREKTORAT PENDIDIKANTim Pengembangan Jurnal Universitas AirlanggaKampus C Mulyorejo Surabaya

EDITORIAL BOARD

EDITORIAL BOARD OF DENTAL JOURNAL (MAJALAH KEDOKTERAN GIGI), May 2nd, 2011 – May 2nd,

2013

SK : 53/H3.1.2/KD/2011

May 2nd, 2011 – May 2nd, 2013

Patron :

Dean of Faculty of Dentistry Airlangga University

Advisors :

Vice Dean I, Vice Dean II, Vice Dean III

Chief Editor :

Ketut Suardita, drg.,Ph.,Sp.KG

(Department of Conservative Dentistry – Airlangga University)

Editorial Boards :

Prof. Dr. M Rubianto, drg, M.S., Sp. Perio. (Periodontic – Airlangga University)

Prof. Nairn Hutchinson Fulton Wilson, MSc. Ph.D., FDS. (Conservative Dentistry - University of Guy’s

Dental School, London)

Prof. W.J. Spitzer, DMD., MD. (Head Department of Cranio & Oral – Maxillofacial Surgery Hamburg

University, Germany)

Prof. Edward C. Combe. M.Sc. Ph.D. D.D.Sc. (Biomaterial – Minnesota University, U.S.A)

Prof. Madya. H. AB. Rani Samsudin D.D.S., FDSRC, AM. (Oral and Maxillofacial Surgery -University

Science Malaysia, Malaysia)

Prof. Taizo Hamada, D.D.S., Ph.D. (Prostodontic - University of Hiroshima, Japan)

Prof. Yukio Kato, D.D.S., Ph.D. (Oral Bio Chemistry-University of Hiroshima, Japan)

Prof. dr. A.G.M. Tielens (Medical Microbiology and Infections Disease - Erasmus University Medical

centre, Rotterdam, the Netherlands)

Prof. Lakshman Samaranayake (Oral Microbiology – The University of Hongkong)

Prof. Kozai Katsuyuki, DDS.,Ph.D. (Pediatric - University of Hiroshima, Japan)

Prof. dr. A.G.M. Tielens (Medical Microbiology - University and Infections Diseases - erasmus

University Medical Centre, Rotterdam, The Netherlands)

Kok Van Kessel (Medical Microbiology and Infections Disease - Erasmus University Medical centre,

Rotterdam, the Netherlands)

Dr. Leslie Ang (Restorative Dentistry – National Dental Centre of Singapore)

Prof. Widowati Witjaksono, drg., Ph.D. (Kulliyah of Dentistry, International Islamic University

Malaysia)

Prof. Dr. M. Suharsini, drg, M.S., Sp. KGA. (Pediatric Dentistry - Indonesia University)

Achmad Gunadi, drg, M.S., Ph.D. (Prostodontic – Jember University)

Managing Editors :

Prof. Dr. Arifzan Razak, drg., MSc.,Sp.Prost (Prosthodontic-Airlangga University)

Prof. Dr. Latief Mooduto, drg, M.S., Sp. KG. (Conservative Dentistry – Airlangga University)

Prof. Dr. Mieke Sylvia M. A. R., drg, M.S.,Sp.Ort. (Ortodontic – Airlangga University)

Prof. Dr. Istiati Soehardjo, drg, M.S. (Oral Biology – Airlangga University)

Prof. R.M. Coen Pramono Danudiningrat, drg.,SU.,Sp.BM. (Oral Maxillofacial Surgery – Airlangga

University)

Prof. Dr. Anita Yuliati, drg, M.Kes. (Dental Material – Airlangga University)

Prof. Seno Pradopo, drg, S.U., Ph.D. Sp. KGA. (Pediatric Dentistry – Airlangga University)

Prof. Dr. Diah Savitri Ernawati, drg., MSi. (Oral Medicine – Airlangga University)

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Page 3: UNIVERSITAS AIRLANGGA DIREKTORAT PENDIDIKAN Tim …journal.unair.ac.id/filerPDF/dentjcc17109b1babs.pdf · 2014. 4. 7. · Fakultas Kedokteran Gigi Abstract Background: Biofilm is

UNIVERSITAS AIRLANGGA DIREKTORAT PENDIDIKANTim Pengembangan Jurnal Universitas AirlanggaKampus C Mulyorejo Surabaya

Thalca I. Agusni, drg, MHPEd. Ph.D.,Sp.Ort. (Ortodontic – Airlangga University)

Dr. R. Darmawan Setijanto, drg., M.Kes. (Dental Public Helath - Airlangga University)

Dr. Elly Munadziroh, drg., MS (Dental Material-Airlangga University)

Priyawan Rachmadi, drg, Ph.D. (Dental Material – Airlangga University)

Dr. Retno Pudji Rahayu, drg., M.Kes. (Oral Biology –Airlangga University)

Markus Budi Rahardjo, drg, M.Kes. (Microbiology – Airlangga University)

Endang Pudjirochani, drg, M.S., Sp. Pros. (Prostodontic – Airlangga University)

Bagus Soebadi, drg, MHPEd. (Oral Medicine –Airlangga University)

Susy Kristiani, drg., M.Kes. (Oral Biology –Airlangga University)

Udijanto Tedjosasongko, drg, Ph.D.,Sp.KGA. (Pediatric Dentistry – Airlangga University)

Devi Rianti, drg., M.Kes. (Dental Material – Airlangga University)

Ira Widjiastuti, drg, M.Kes.Sp.KG. (Consevative Dentistry –Airlangga University)

Sianiwati Goenharto, drg., M.S. (Ortodontic –Airlangga University)

Dr. Chiquita Prahasanti, drg., Sp.Perio. (Periodontic – Airlangga University)

Dr. Eha Renwi Astuti, drg.,M.Kes. (Roentgen – Airlangga University)

Rostiny, drg., M.Kes.,Sp.Pros. (Prosthodontic – Airlangga University)

Annisaa Chusida, drg., M.Kes. (Oral Biology-Airlangga University)

Eric Priyo Prasetyo, drg.,Sp,KG.(Conservative Dentistry –Airlangga University)

Administrative Assistant :

Novi Dian Prastiwi (Faculty of Dentistry – Airlangga University)

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Page 4: UNIVERSITAS AIRLANGGA DIREKTORAT PENDIDIKAN Tim …journal.unair.ac.id/filerPDF/dentjcc17109b1babs.pdf · 2014. 4. 7. · Fakultas Kedokteran Gigi Abstract Background: Biofilm is

UNIVERSITAS AIRLANGGA DIREKTORAT PENDIDIKANTim Pengembangan Jurnal Universitas AirlanggaKampus C Mulyorejo Surabaya

Table of Contents

No Title Page

1 The role of Actinobacillus actinomycetemcomitans fimbrial adhesin on MMP-8

activity in aggressive periodontitis pathogenesis

181 -

186

2 Aesthetic treatment on anterior teeth crown fracture caused by dental trauma 187 -

192

3 Molecular characterization of interleukin-1A (+4845) GTgene in aggresive

periodontitis patients

193 -

197

4 Antitumor activity of intratumoral injection of pcDNA3.1-p27Kip1mt followed by in

vivo electroporation in a malignant Burkitt’s lymphoma cell xenograft

198 -

202

5 The effect of nickel as a nickel chromium restoration corrosion product on gingival

fibroblast through analysis of BCl-2

203 -

208

6 Effect of Citrus aurantifolia swingle essential oils on methyl mercaptan production of

Porphyromonas gingivalis triggering halitosis

209 -

213

7 Changes in setting time of alginate impression material with different water

temperature

215 -

218

8 Inhibition effect of cashew stem bark extract (Anacardium Occidentale L.)

on the inhibition of biofilm formation of Streptococcus sanguinis

214 -

218

9 Inhibition of dental plaque formation by toothpaste containing propolis 219 -

222

10 Effectiveness of various sterilization methods of contaminated post-fitted molar

band

223 -

226

11 Dental student’s perception to Aesthetic Component of IOTN and demand for

orthodontic treatment

227 -

230

12 The inhibition of malignant epithelial cells in mucosal injury in the oral cavity of

strains by pomegranate fruit extract (Punica granatum linn) through Bcl-2

expression

231 -

234

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Page 5: UNIVERSITAS AIRLANGGA DIREKTORAT PENDIDIKAN Tim …journal.unair.ac.id/filerPDF/dentjcc17109b1babs.pdf · 2014. 4. 7. · Fakultas Kedokteran Gigi Abstract Background: Biofilm is

UNIVERSITAS AIRLANGGA DIREKTORAT PENDIDIKANTim Pengembangan Jurnal Universitas AirlanggaKampus C Mulyorejo Surabaya

Vol. 45 - No. 4 / 2012-12

TOC : 7, and page : 214 - 218

Inhibition effect of cashew stem bark extract (Anacardium Occidentale L.) on the inhibition of biofilm

formation of Streptococcus sanguinis

Inhibition effect of cashew stem bark extract (Anacardium Occidentale L.) on the inhibition of biofilm

formation of Streptococcus sanguinis

Author :

Rizni Amaliah | [email protected]

Mahasiswa Fakultas Kedokteran Gigi

Sri larnani | [email protected]

Fakultas Kedokteran Gigi

ivan arie Wahyudi | [email protected]

Fakultas Kedokteran Gigi

Abstract

Background: Biofilm is communities of microorganisms attached to solid surface and enclosed

in extracellular matrix that protected microorganisms from antibacterial agents and host defense.

One of bacteria might have a role in initial colonization of biofilm formation is Streptococcus

sanguinis (S. sanguinis). Previous studies showed that cashew stem bark extract (Anacardium

occidentale L.) can inhibit the growth of Streptococcus strains. Purpose: The purpose of this study

was to determine the inhibition effect of cashew (Anacardium occidentale L.) stem bark ethanol

extract on biofilm formation of S. sanguinis. Methods: Streptococcus sanguinis grown in Brain Heart

Infusion (BHI) + 2% sucrose medium by using microplate polystyrene 96 wells. The samples were

divided into 3 groups, 5% polyethyleneglycol (PEG) as negative control, cashew stem bark extract

(concentration 3.125 mg/ml, 6.25 mg/ml, 9.375 mg/ml, and 12.5 mg/ml), and 0.12% chlorhexidine

(as positive control). Biofilm was stained by 1% crystal violet. Afterwards, optical density (OD) of

samples were measured by microplate reader λ595 nm. The data of biofilm formation inhibition

percentage were analyzed by one way ANOVA and then continued by Least Significant Difference

(LSD) test. Results: The result of one way ANOVA showed that there were significant differences in

inhibition of S. sanguinis biofilm formation (p<0.05). LSD test showed that concentration extract

3.125 mg/ml had significant difference with concentration 9.375 mg/ml and 12.5 mg/ml.

Reciprocally, concentration 6.25 mg/ml had significant difference with concentration 9.375 mg/ml

and 12.5 mg/ml. Conclusion: Cashew stem bark extract was able to inhibit biofilm formation of S.

sanguinis.

Keyword : Inhibition, of, biofilm, formation, Streptococcus, sanguinis, cashew, stem, bark,

Daftar Pustaka :

1. Socransky SS, (1994). Evidence of bacterial etiology: A historical perspective. - :

Periodontology 2000

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