UHF Breakthrough: First High Resolution 1.2 GHz NMR ......gave rise to large initial field...
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For the first time, a 1.2 GHz NMR magnet has been energized to full field (Figure 1). Bruker’s 1.2 GHz NMR magnet is a hybrid magnet, consisting of both LTS (NbTi and Nb3Sn) and HTS (REBCO) sections. The homogenization of HTS magnets is particularly time-consuming, as screening currents in the HTS conductor gave rise to large initial field imperfections, and also reduced the efficiency of conventional cryogenic shim coils. The homogenization was successful and Bruker BioSpin is now recording the first 1.2 GHz NMR spectra, has a second 1.2 GHz magnet at full field, and is currently constructing two more 1.2 GHz magnets. UHF Breakthrough: First High Resolution 1.2 GHz NMR Spectra Reached Figure 1: The first 1.2 GHz magnet to reach full field. This photo shows the magnet in Bruker BioSpin’s test laboratory in Fällanden, Switzerland, a few days after the initial energization was completed and the persistent switch closed. Advanced Magnet Design Hybrid HTS/LTS magnet technology with all parts of the magnet electrically connected in series to eliminate coupling effects and achieve superior stability Active shielding to reduce stray fields for easier siting, with integrated suppression against external field disturbances Built-in cryogenic shims for magnetic field homogenization Advanced jointing technology for low drift rates to meet the Figure 2: One of the first 1.2 GHz 1 H spectra of 2 mM sucrose recorded with a 3 mm TCI CryoProbe. Figure 3: Ascend 1.2 GHz NMR hybrid magnet concept with HTS insert and LTS outsert. Figure 4: 1 H- 15 N 2D sofast-HMQC of a 500 µM ubiquitin sample, 13 C/ 15 N labeled in H 2 O:D 2 O 90:10. This data was recorded with a 3 mm TCI Cryo- Probe. Figure 5: 1 H- 13 C ct-HSQC of a 500 µM ubiquitin sample, 13 C/ 15 N labeled in H 2 O:D 2 O 90:10. This data was recorded with a 3 mm TCI CryoProbe. Figure 6: 1 H- 15 N 2D BEST-TROSY of a 500 µM ubiquitin sample, 13 C/ 15 N labeled in H 2 O:D 2 O 90:10. This data was recorded with a 3 mm TCI CryoProbe. Figure 7: 2D plane of a 3D 15 N edited NOESY-HSQC of a 500 µM ubiquitin sample, 13 C/ 15 N labeled in H 2 O:D 2 O 90:10. This data was recorded with a 3 mm TCI CryoProbe. Site Planning Please contact your Bruker sales representative to discuss siting- related aspects of Bruker’s new 1.2 GHz spectrometers.
Transcript of UHF Breakthrough: First High Resolution 1.2 GHz NMR ......gave rise to large initial field...
For the first time, a 1.2 GHz NMR magnet has been energized to full field (Figure 1). Bruker’s 1.2 GHz NMR magnet is a hybrid magnet, consisting of both LTS (NbTi and Nb3Sn) and HTS (REBCO) sections.
The homogenization of HTS magnets is particularly time-consuming, as screening currents in the HTS conductor gave rise to large initial field imperfections, and also reduced the efficiency of conventional cryogenic shim coils.
The homogenization was successful and Bruker BioSpin is now recording the first 1.2 GHz NMR spectra, has a second 1.2 GHz magnet at full field, and is currently constructing two more 1.2 GHz magnets.
UHF Breakthrough: First High Resolution 1.2 GHz NMR Spectra Reached
Figure 1: The first 1.2 GHz magnet to reach full field. This photo shows the magnet in Bruker BioSpin’s test laboratory in Fällanden, Switzerland, a few days after the initial energization was completed and the persistent switch closed.
Advanced Magnet Design�� Hybrid HTS/LTS magnet technology with all parts of the magnet electrically connected in series to eliminate coupling effects and achieve superior stability�� Active shielding to reduce stray fields for easier siting, with integrated suppression against external field disturbances�� Built-in cryogenic shims for magnetic field homogenization�� Advanced jointing technology for low drift rates to meet the Figure 2: One of the first 1.2 GHz 1H spectra of 2 mM sucrose recorded with a 3 mm TCI
CryoProbe.
Figure 3: Ascend 1.2 GHz NMR hybrid magnet concept with HTS insert and LTS outsert.
Figure 4: 1H-15N 2D sofast-HMQC of a 500 µM ubiquitin sample, 13C/15N labeled in H2O:D2O 90:10. This data was recorded with a 3 mm TCI Cryo-Probe.
Figure 5: 1H-13C ct-HSQC of a 500 µM ubiquitin sample, 13C/15N labeled in H2O:D2O 90:10. This data was recorded with a 3 mm TCI CryoProbe.
Figure 6: 1H-15N 2D BEST-TROSY of a 500 µM ubiquitin sample, 13C/15N labeled in H2O:D2O 90:10. This data was recorded with a 3 mm TCI CryoProbe.
Figure 7: 2D plane of a 3D 15N edited NOESY-HSQC of a 500 µM ubiquitin sample, 13C/15N labeled in H2O:D2O 90:10. This data was recorded with a 3 mm TCI CryoProbe.
Site PlanningPlease contact your Bruker sales representative to discuss siting-related aspects of Bruker’s new 1.2 GHz spectrometers.
