TRKB/BDNF SIGNALING PATHWAY AND NEUROPATHIC PAIN

Casey Gilman Ginty Lab

Overview of Neuropathic Pain

TrkB-expressing Neurons

Neubarth, unpublishedAbraira and Ginty, Neuron (2013)

Ret TrkB

The TrkB/BDNF signaling pathway within sensory neurons is necessary for neuropathic pain.

Hypothesis

Upregulation of TrkB expression after induced neuropathic pain

Line: TrkBCreER/+; TaumGFP/+

Start age: 6-8 weeks old

von Frey threshold to

establish baseline

1

Surgery (partial sciatic nerve ligation

or sham)

2

Tamoxifen treatments

3

Retest von Frey to confirm

neuropathic pain

4 Immunohistochemistry

5Start 3-5 days 2 weeks

Tissue-specific knockout of TrkB

AdvillinCreER/+; TrkBGFP/+ TrkBflox/flox AdvillinCreER/+; TrkBflox/TrkBGFP

Behavior of mouse line with deleted Trk B gene before and after sciatic lesion

Line: AdvillinCreER/+; TrkBflox/TrkBGFP

Tam: ~P21

TAM at P211

Use TrkB antibody/in-

situ hybridization

to ensure knockout

2

Using new set of mice but

same line, TAM at P21

3

Test von Frey to establish

baseline 4

Partial sciatic nerve ligation

5

Start 2 weeksRetest von Frey and compare knockout vs.

control animals

61-7 days

Acknowledgements Nicole Neubarth

Brendan Lehnert

David Ginty

Ling Bai

Pann Nwe

Katerina Mesa