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Training on

Forensic Molecular Biology, Biotechnology, DNA Fingerprinting and DNA Barcoding

Organized by

Dr.Prem P Jauhar Laboratories of Molecular Sciences

New Delhi India-110092

1st June 2012 to 30th June 2012

Forensic Molecular Biology, Biotechnology, DNA Fingerprinting and DNA

Laboratories of Molecular Sciences

110092 June 2012

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BACKGROUND

Realizing the importance of forensic molecular biology, DNA fingerprinting, diagnostics in human health care, molecular breeding, functional biomedical genetics, drugand gene therapy with application of evolutionary studies and the genetic engineeringJauhar Laboratories of Molecular Sciences, New Delhi India is take initiative at international levelTraining On Forensic Molecular Biology, Biotechnology, DNA Fingerprinting And main aim of this training is to provide hands on experience to the various prospects of this field and share opportunities among scientists, engineers, doctors, research scholars andand abroad. The training will provide an excellent platform for engineering, molecular biology, forensic, evolutionary biology, molecular breeding, genetically modified crop/organism/animal (GMOs, LMOs and transgenic) and medical backgroundtake the challenges in rapid development of this emerging field across the globebiotechnology, biosystematics, livestock genetic diversity, marine biodiversity and biotechnology, marine and fresh water fish biodiversity, ornithological studies, fisheries biotechnology,agro entomology, integrated biological control ,sericulture biotechnology,property rights and patents, animal and veterinary products, conservation strategies and anthropological molecular survey studies.

fingerprinting, DNA barcoding, and DNA diagnostics in human health care, molecular breeding, functional biomedical genetics, drug development

evolutionary studies and the genetic engineering. The Dr.Prem P take initiative at international level organizing Fingerprinting And DNA Barcoding 2012. The

main aim of this training is to provide hands on experience to the various prospects of this new emerging field and share opportunities among scientists, engineers, doctors, research scholars and students in India

raining will provide an excellent platform for work and share them with science, molecular breeding, genetically modified

and medical background and help to come together, take the challenges in rapid development of this emerging field across the globe toward the biodiversity biotechnology, biosystematics, livestock genetic diversity, marine biodiversity and biotechnology, marine

fisheries biotechnology, insects and pests diversity, ntegrated biological control ,sericulture biotechnology, animal medicine, intellectual

animal nutrition and feed technology, and anthropological molecular survey studies.

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Training Theme

1. General Laboratory Experiment : (Hands on): DNA isolation from Plant, Bacteria, Yeast, Fungi , Animal Tissue (Heart, Kidney, Lever, and Blood etc), Quantification and Quality check of isolated DNA, DNA Dilution, PCR Amplification of DNA using Multiplex Primers, PCR Amplification of DNA using random 10mer primers for RAPD, PCR Amplification of DNA using SSR primers, Micro Satellite Analysis and STR Primer for DNA Fingerprinting, Separation Following Automated electrophoresis, Data Capfingerprint development.

2. Human DNA Fingerprinting and Identification :Laboratory Experiments (human blood, quantification and quality check of isolated DNA, DNA dilution, PCR amplification multiplex primers, fragment (allele) separation following electrophoresis, data capturing, data analysis and DNA fingerprint development

3. DNA Fingerprinting of Plants following Random Amplified Polymorphic DNA (RAPD) and Microsatellite techniques: Laboratory Experiments (Hands on): Isolation of DNA, quantification and quality check of isolatedDNA, DNA dilution, PCR amplification of DNA using random 10‐ merDNA using SSR/ISSR/STR primers for microsatellite analysis, agarose gel electrophoresis of PCR products for RAPD, allele separation following automated capillary electrophoresis for microsatellite analysis, data collection and data analysis

DNA isolation from Plant, Bacteria, Yeast, Fungi , Animal Tissue (Heart, Kidney, Lever, and Blood etc), Quantification and Quality check of isolated DNA, DNA Dilution,

DNA using Multiplex Primers, PCR Amplification of DNA using random 10mer primers for RAPD, PCR Amplification of DNA using SSR primers, Micro Satellite Analysis and STR Primer for DNA Fingerprinting, Separation Following Automated electrophoresis, Data Capturing, Data Analysis and DNA

Laboratory Experiments (Hands on): DNA isolation from human blood, quantification and quality check of isolated DNA, DNA dilution, PCR amplification of DNA using multiplex primers, fragment (allele) separation following electrophoresis, data capturing, data analysis and DNA

DNA Fingerprinting of Plants following Random Amplified Polymorphic DNA (RAPD) and Microsatellite Laboratory Experiments (Hands on): Isolation of DNA, quantification and quality check of isolated

mer primers for RAPD, PCR amplification of primers for microsatellite analysis, agarose gel electrophoresis of PCR products for

RAPD, allele separation following automated capillary electrophoresis for microsatellite analysis, data collection

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4. DNA barcoding of animals using Cytochrome C Oxidase subunit I (COI):DNA isolation, quantification and quality check of the isolated DNA, DNA dilution, PCR amplification of DNA using COI primers, agarose gel electrophoresis of PCR products, gel elution products and sequence data analysis.

5. Forensic Molecular Biology: DNA Fingerprinting method of plant, animals, microorganisms, human and Diagnostics (Disease Diagnostics, Heritable Disease Diagnostics, Cancer DiagnosParental Diagnostic, and Mass Disaster of Natural Calamities and Army Causality.

6. DNA Fingerprinting: Methods and protocols used in DNA Fingerprinting of Human, DNA Fingerprinting of animals, DNA Fingerprinting of Fishes, DNA Fingerprinting of plant, DNA Fingerprinting of fungi, Bacteria, and Yeast etc. Implication and issues with techniques.

7. DNA Bar-coding: Methods used in Human DNA Barcoding, Animal DNA Barcoding, Insect DNA Barcoding, Fish DNA Barcoding and microorganism DNA Barcoding, Plant DNA Barcodingtechniques etc.

8. Phylogenic Analysis: Molecular Phylogenic Analysis, Genotyping of Plant, Animal, Fish, Insect and Microbial Population, Individual and Community, Seed Purity, Genetic Containment, Germplasm Characterization, Allele and Gene Discovery, Implication and issues with technique etc.

9. Genetic Engineering: Gene Isolation, Gene Detection, GMOs,Microbial Contamination Determination.

Cytochrome C Oxidase subunit I (COI):Laboratory experiments (Hands on): DNA isolation, quantification and quality check of the isolated DNA, DNA dilution, PCR amplification of DNA using COI primers, agarose gel electrophoresis of PCR products, gel elution of PCR products, sequencing of PCR

DNA Fingerprinting method of plant, animals, microorganisms, human and Diagnostics (Disease Diagnostics, Heritable Disease Diagnostics, Cancer Diagnostics, Pathogen Diagnostics, Parental Diagnostic, and Mass Disaster of Natural Calamities and Army Causality.

Methods and protocols used in DNA Fingerprinting of Human, DNA Fingerprinting of DNA Fingerprinting of plant, DNA Fingerprinting of fungi, Bacteria, and

Methods used in Human DNA Barcoding, Animal DNA Barcoding, Insect DNA Barcoding, ganism DNA Barcoding, Plant DNA Barcoding, Implication and issues with

Molecular Phylogenic Analysis, Genotyping of Plant, Animal, Fish, Insect and Microbial etic Containment, Germplasm Characterization, Allele and

GMOs, LMOs Detection, GMO Food Detection,

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10. Molecular Marker Technology: SSR, ISSR, RAPD, STR, Molecular Marker Assisted Selection, Molecular Breeding, Cultivar Development, Molecular Mapping, Gene Mapping, Genetic Structure Analysis, Molecular Marker Development.

11. Biotechnology: Nucleic Acid Extraction, Purification, Dilution, Spectrophotometer Quantization, electrophoresis, DNA Elution, PCR, PCR Product Purification, Genetic (DNA) Fingerprint, DNA Molecular Profile Development.

12. DNA Fingerprinting Software and Biostatistics Analysis: DNA Fingerprinting Profile Regeneration, DNA Barcoding, Diversity Matrix Development, Phylogenetic Tree Development, Allele Frequency, Private Allele, Banding Pattern, Heterozygosis, Primer discriminatory Power Test, and molecular Biostatistics

13. Result Analysis and Interpretation: on Forensic Molecular Biology, Molecular genetics, Evolutionary aspects and Phylogenetic background.

Technology: SSR, ISSR, RAPD, STR, Molecular Marker Assisted Selection, Molecular Mapping, Gene Mapping, Genetic Structure Analysis, Molecular

Nucleic Acid Extraction, Purification, Dilution, Spectrophotometer Quantization, electrophoresis, DNA Elution, PCR, PCR Product Purification, Genetic (DNA) Fingerprint, DNA Molecular Profile

DNA Fingerprinting Profile Regeneration, DNA Barcoding, Diversity Matrix Development, Phylogenetic Tree Development, Allele Frequency, Private Allele, Banding Pattern, Heterozygosis, Primer discriminatory Power Test, and molecular Biostatistics analysis.

n Forensic Molecular Biology, Molecular genetics, Evolutionary aspects

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IMPORTANT DATES First Batch

Application Deadline 15 th May, 2012 Acceptance of Application 15 th May, 2012 Acceptance of Application for Abroad candidate with fee submission 25

Second Batch Application Deadline 25th June, 2012 Acceptance of Application for Second Batch 25 th June 2012. All fee will be accepted through bank transfer only

REGISTRATION FEES

First Batch 1st June to 30th June 2012 Second Batch Before 15th May, 2012 Foreign Indian Before 15th May, 2012Govt. Faculty/Scientist 2000 USD 18000 INR Govt. Faculty/ScientistPrivate Professionals/ NGO 2000 USD 18000 INR Private Professionals/ NGOStudent 1000 USD 11000 INR Student After 15th May, 2012 Foreign Indian After 25Govt. Faculty/Scientist 2050 USD 18500 INR Govt. Faculty/ScientistPrivate Professionals/ NGO 2050 USD 18500 INR Private Professionals/ NGOStudent 1025 USD 11300 INR Student

*Registration Fees is inclusive of Training Kit, Abstract Book, Proceedings

Candidate need to deposit their all fee though Bank only with detail given to registration form and /or confirmation letter

Acceptance of Application for Abroad candidate with fee submission 25th may 2012

June 2012. accepted through bank transfer only

Second Batch 1st July to 30th July 2012 Before 15th May, 2012 Foreign Indian Govt. Faculty/Scientist 2000 USD 18000 INR Private Professionals/ NGO 2000 USD 18000 INR

1000 USD 11000 INR 25th June, 2012 Foreign Indian

Govt. Faculty/Scientist 2050 USD 18500 INR Private Professionals/ NGO 2050 USD 18500 INR

1025 USD 11300 INR

Kit, Abstract Book, Proceedings

with detail given to registration form and /or confirmation letter

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Registration form Dr.Prem P Jauhar Laboratories of Molecular Sciences

Patparganj Industrial Area near Anand Vihar ISBT New Delhi India 110092

“Training on Forensic Molecular Biology, Biotechnology, DNA Fingerprinting and DNA Barcoding”

Full Name: ……………………………………………………………………………………………………………………Designation: …………………………………………………………………………… …………………………………Institution: …………………………………………………………………… …………………………………Address with Pin Code: ………………………………………………… …………………………………………………………………… ………………………………… …………………………………a) Official: ……………………………………………………………………………… ……………………………………………………………………………………………………………… …………………………………b) Residence: ………………………………………… ………………………………… ……………………………………………………………………………………………………………………………………………………………………………………………………Cell Phone: ………………………… Fax: ……………………………E-Mail: ……………………………………Telephone with area Code: …………Registration Fees paid details: INR/USD…………………………………………… Bank Transfer Accommodation on Request only : Hotel A/C / Non A/C /PG

Bank Detail:- Punjab National Bank- A/C -2972001500001162 (IFSC CODE - PUNB0297200. MICR CODE: Branch CODE:

Patparganj Industrial Area near Anand Vihar ISBT New Delhi India 110092Contacts- 91+9560757331,

Signature Place :

Laboratories of Molecular Sciences Patparganj Industrial Area near Anand Vihar ISBT New Delhi India 110092

“Training on Forensic Molecular Biology, Biotechnology, DNA Fingerprinting and DNA

………………………………………………………………………………………………………………………………………………… ………………………………… ………………………………

………………………………… ………………………………… ……… ………………………………… ………………………………………………

………………………………… ……………………………………………… …… ………………………………… …………………………………

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…………………………………………………………………………………………………………………… Mail: ……………………………………Telephone with area Code: ………………

Bank Transfer with date: ………………………………………………… ……

PUNB0297200. MICR CODE: Branch CODE: 297200.) Name of Beneficiary -Vinay Kumar Singh E-Mail the Registration form to :

Vinay Kumar Singh Director Training and Projects,

Dr.Prem P Jauhar Laboratories of Molecular Sciences Patparganj Industrial Area near Anand Vihar ISBT New Delhi India 110092

91+9560757331, 8802065581, 9899242448, 9716159717, 7830959033 Email-ppjauharlabs@gmail.com mr.vksinghs@gmail.com

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