thE world’S moSt AdvAncEd mEtABolic...
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thE world’S moSt AdvAncEd mEtABolic AnAlyzErS
Seahorse XFe Extracellular Flux Analyzers
cancer immunology Stem cell Biologycardiovascular disease neurodegeneration toxicology and hepatobiologycell physiology obesity, diabetes, and metabolic disorders translational medicine
the role of metabolism in cellular and physiological processes, from cardiovascular function to immune
response, is well established; many diseases are now linked to metabolic dysfunction or reprogramming.
using the Seahorse XFe Extracellular Flux Analyzer, scientists are now able to easily obtain powerful
functional metabolic data to gain a greater understanding of cell metabolism and enable new
advancements in life science research.
Functional Metabolic Data on Live Cells in Real Time
the Seahorse XF cell Energy phenotype test measures the key parameters used to determine the energy phenotype of a cell: baseline phenotype, stressed phenotype, and metabolic potential.
the Seahorse XF glycolysis Stress test reports three key parameters of glycolytic function: glycolysis, glycolytic capacity, and glycolytic reserve.
the Seahorse XF Fatty Acid oxidation assay measures both exogeneous palmitate oxidation and endogenous fatty acid oxidation.
the Seahorse XF cell mito Stress test measures the key parameters of mitochondrial function: basal respiration, Atp production, proton leak, maximal respiration, and spare respiratory capacity.
Extracellular Acidification Rate (ECAR)
Oxy
gen
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sum
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ate
(OC
R)
Glycolysis
XFp Cell Energy Phenotype Profile
Mito
chon
dria
l Res
pira
tion
Aerobic
Quiescent
Energetic
Glycolytic
BaselinePhenotype
StressedPhenotype
Metabolic Potentia
l
Oxy
gen
Con
sum
ptio
n R
ate
(OC
R)
Seahorse XF Fatty Acid Oxidation AssayExogenous & Endogenous Fatty Acid oxidation
Oxy
gen
Con
sum
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ate
(OC
R)
Oxy
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Con
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(OC
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Seahorse XF Glycolysis Stress Testglycolytic Function
Seahorse XF Cell Mito Stress Testmitochondrial respiration
TIME (minutes)
(mpH
/min
)
Glycolytic Function
Glucose Oligomycin 2-DG
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5
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0 20 40 60 8010 30 50 70 90 100
Non-glycolytic Acidification
Glycolysis
GlycolyticCapacity
GlycolyticReserve
(pm
ol/m
in)
TIME (minutes)
180
160
140
120
100
80
60
40
20
00 10 20 30 40 50 60
Rotenone & antimycin A
Oligomycin FCCP
ExogenousPalmitate Oxidation
ExogenousPalmitate Oxidation
Endogenous FAO
Basal
Maximal
XF Fatty Acid Oxidation Profile
Endogenous FAO
TIME (minutes)
(pm
ol/m
in)
Mitochondrial Respiration
Oligomycin FCCP Rotenone & antimycin A
0
120
80
40
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0 20 40 60 8010 30 50 70 90 100 110
Non-mitochondrial Oxygen Consumption
BasalRespiration
ATPProduction
Proton Leak
MaximalRespiration
SpareCapacity
Extr
acel
lula
r Aci
dific
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n R
ate
(EC
AR
)
Extracellular Acidification Rate (ECAR)
SeahorSe XF aSSayS — The Gold STandard For MeTabolic MeaSureMenTS
Seahorse XF Cell Energy Phenotype Testmetabolic phenotype & potential
CancerSeahorse XF glycolysis Stress test reveals mechanism of action of cancer pathway inhibition.
Obesity/DiabetesSeahorse XF technology reveals hedgehog pathway-induced switch to a glycolytic phenotype in adipocytes.
NeurodegenerationSeahorse XF cell mito Stress test reveals metabolic signatures of embryonic stem cells with huntington mutations.
ImmunologySeahorse XF cell mito Stress test demonstrates fatty acid oxidation is required for memory t cell formation.
viale et al., (2014) oncogene ablation-resistant pancreatic cancer cells depend on mitochondrial function. nature. 514(7524):628-32.
50
P < 0.01
4540353025201510
5
5 10 15 20 25 30 35 40 45 50 55 600
Control
PI3K/mTOR&MEK1PathwayInhibitors
GlucoseOligomycin
2DG
Aerobic
Glycolytic
MoreMetabolic
LessMetabolic
160
Control
SAG
140
120
1004 5 6 7
800
Control orEtomoxir Oligomycin
IL-15 + controlIL-15 + etomoxir
FCCPRotenone/
antimycin A
400
600
200
10025 50 75 100 120
Functional metabolic data is required to tell a complete story of cellular processes and pathologies.
the Seahorse XFe Extracellular Flux Analyzer provides the capability to examine the intact and functional
cellular system, enabling comprehensive investigations into metabolic pathways, substrate preference and
utilization, catabolic and anabolic processes, and metabolic phenotypes.
SeahorSe XF TechnoloGy — GeneraTinG new inSiGhTS inTo diSeaSeo
xyge
n c
onsu
mpt
ion
rat
e (o
cr
)(p
mol
/min
)
oxy
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(oc
r)
(pm
ol/m
in)
oxy
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ate
(oc
r)
(pm
ol/m
in)
Extracellular Acidification Rate (ECAR) (mph/min)
Ext
race
llula
r Aci
dific
atio
n R
ate
(EC
AR
) (m
ph/m
in)
time (min) time (min)
time (min)
600
500
Oligomycin
Embryonic lethal mutationControl
FCCP Rotenone/antimycin A
300
400
200
100
0100 20 30 40 50 60 70
Dominantly inherited Huntington’s Disease
human pancreatic tumor cells human pancreatic tumor cells
murine adipocytes murine embryonic stem cells
van der windt et al., (2012) mitochondrial respiratory capacity is a critical regulator of cd8+ t cell memory development. immunity. 36(1): 68-78.
teperino et al., (2012) hedgehog partial agonism drives warburg-like metabolism in muscle and brown fat. cell. 151(2): 414-26.
ismailoglu et al., (2014). huntingtin protein is essential for mitochondrial metabolism, bioenergetics and structure in murine embryonic stem cells. dev. Biol. 391(2): 230-40.
How XF Technology Works
the Seahorse XFe Analyzer simultaneously measures the two major energy pathways of the cell — mitochondrial respiration and glycolysis — in live cells using a label-free, solid-state sensor cartridges in a microplate format. the Seahorse XFe Analyzer works with all cell types, including primary cells, cell lines, suspension cells, as well as islets, C. elegans, yeast, and isolated mitochondria.
thE pAtEntEd microchAmBEr mAkES it All poSSiBlE
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integrated injection ports sequentially deliver up to 4 compounds allowing dose response, agonist or antagonist response, or pathway perturbation analysis of each sample.
Sensor probes gently lower to create a transient microchamber, allowing rapid, real time measurement of changes in both oxygen and proton concentrations in the extracellular medium.
Seahorse XF cell culture microplates support virtually all cell types.
inert optical micro sensors measure rates of oxygen consumption and extracellular acidification simultaneously.
the microplate well requires a small number of cells, 10-20 fold fewer cells compared to conventional respirometers.
I
II
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IV
Lactate
Pyruvate
Glucose
GLYCOLYSIS — ECAR (Extracellular Acidification Rate)
cells generate Atp via glycolysis independent of oxygen, producing lactic acid and protons.
the Seahorse XFe Analyzer measures glycolysis by measuring the extracellular acidification rate (ECAR) of cells.
MITOCHONDRIAL RESPIRATION —
OCR (Oxygen Consumption Rate)
mitochondria consume oxygen when oxidizing fatty
acids or other substrates to generate Atp. the Seahorse
XFe Analyzer measures mitochondrial respiration by measuring the oxygen
consumption rate (ocr) of cells.
the Seahorse XFe Analyzer utilizes patented transient microchambers which enable sensitive, precise, and nondestructive metabolic measurements in real time.
Cutaway graphic of a single probe
and well
BC
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A A
Seahorse XF Data in Publications there are over 2,000 papers utilizing Seahorse XF technology published in leading scientific journals such as nature, cell, Science and pnAS. Scientists are embracing Seahorse XF technology to identify metabolic phenotypes and reprogramming, as well as target metabolic changes for therapeutic purposes.
Built on innovative and proven Seahorse XF technology, the Seahorse XFe Extracellular Flux Analyzer
makes it easy to perform functional metabolic measurements in live cells, in real time. A complete platform
that includes hardware, software, consumables, and reagents, the Seahorse XFe Analyzer is a powerful
and flexible system that is accelerating life science research around the world.
complEtE plAtForm For mEtABolic ASSAyS
powErFul AnAlyticS
EASy-to-uSE kitS And
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FunctionAl livE cEll ASSAyS
provEn, EnABling
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EXpErt trAining And
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NEW
DISCOVERIES
© 2016 All rights reserved. Seahorse Bioscience and the Seahorse logo are trademarks of Seahorse Bioscience inc. All other trademarks and registered trademarks are the property of their respective owners. the content in this brochure is for informational purposes only and may be subject to change without notice.
Bringing a new perspective to cEll mEtABoliSm rESEArch
Specifications Seahorse XFe24 Analyzer Seahorse XFe96 Analyzer
Dimensions 41cm x 61cm x 43cm(16" x 24" x 17" )
41cm x 61cm x 43cm(16" x 24" x 17" )
Weight 20 kg (45 lb) 14 kg (35 lb)
Power Requirements 100-240vAc50/60hz
100-240vAc50/60hz
Primary Measurements Made ocr & EcAr ocr & EcAr
Assay Format live cells in a specialized 24-well microplate
live cells in a specialized 96-well microplate
Software
wave supports assay design, instrument control, and data analysis. can be installed on windows® pc for analysis or data export to mS® Excel™ or graphpad prism®.
wave supports assay design, instrument control, and data analysis. can be installed on windows® pc for analysis or data export to mS® Excel™ or graphpad prism®.
Injection Ports 4 per well, 75 µl each 4 per well, 25 µl each
Assay Running Volume 500-1000 µl/well 80-200 µl/well
Sample Requirements 10,000-1,000,000 cells/well 4,000-500,000 cells/well
Instrument Controllercombination computer and touch-screen display with full assay design, control, and analysis capability.
combination computer and touch-screen display with full assay design, control, and analysis capability.
Best For/Key Advantages islets
Maximum experimental flexibilityhighest throughput Spheroids dose-response studies
cancer immunology Stem cell Biologycardiovascular disease neurodegeneration toxicology and hepatobiologycell physiology obesity, diabetes, and metabolic disorders translational medicine
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Corporate Headquarters
Seahorse Bioscience inc.16 Esquire roadnorth Billerica, mA 01862 uSphone: +1 978 671 1600www.seahorsebio.com
European Headquarters
Seahorse Bioscience EuropeFruebjergvej 32100 copenhagen dkphone: +45 31 36 98 78
Asia-Pacific Headquarters
Seahorse Bioscience Asia199 guo Shou Jing rd, Suite 207pudong, Shanghai 201203 cnphone: 0086 21 33901768