The Effect of Different Cleaning Procedures on the Hygiene of Hands
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The Effect of Different Cleaning Procedures on the Hygiene of Hands By: Chelsea Guan Pd. 10
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The Effect of Different Cleaning Procedures on the Hygiene of Hands . By: Chelsea Guan Pd. 10. Problem Statement and Hypothesis. What is the most effective way to remove bacteria from hands? - PowerPoint PPT Presentation
Transcript of The Effect of Different Cleaning Procedures on the Hygiene of Hands
The Effect of Different Cleaning Procedures on the Hygiene of Hands
By: Chelsea GuanPd. 10
Problem Statement and HypothesisWhat is the most effective way to remove
bacteria from hands?If hands are washed with soap and water,
then there will be fewer bacteria left than if hand sanitizer or water were used.
Basic ConceptsPathogenAsepticAgarIncubation
ProcedureGather materials (buy Agar Plates, soap,
hand sanitizer) Find 9-12 people to volunteer for the test
(no sex or age requirement) Divide the volunteers into 3 groupsLabel each plate with the words left and
right, the name of the volunteer, and which group the plate belongs to
Each volunteer will then lightly press their left hand in an Agar Plate
Procedure (Continued)They will then wash both their hands with
soap, hand sanitizer, or water respectively. (For soap and water, hands must covered in soap and rinsed under warm water for 20 seconds. For hand sanitizer, a dime sized drop of hand sanitizer will be placed on the left palm. Hands will then be rubbed together for 20 seconds. For water alone, hands will be rinsed under warm water for 20 seconds.)
Each volunteer will then press their right hand in the same plate respectively
Procedure (Continued)Seal plates using parafilm and tapeThe plates will then be incubated for 2 days
inside an incubator inside of conditions (temperature around 20-23 degrees C)
Each plate will then be taken out (PLATES ARE NOT TO BE OPENED), and bacteria colonies on the Agar Plates will be counted
To prevent spread of possible pathogen, plates will be disposed after autoclaved.
Design DiagramLevels Soap and
WaterWater Hand
SanitizerNone
Trials 3 3 3 9
DataLevels Soap and
WaterWater Hand
SanitizerNone
Trials 0 4 1 22 1 1 30 2 2 361 0
10004426
Analyzed ResultsSoap and Water
Water Hand Sanitizer
None
Observed .75 2.33 1.33 4.833Expected 2.3125 2.3125 2.3125 2.3125Total 1.06 .00013 .42 2.75
P value: 4.23
Photo Documentation
Graphs of Data
Series10
0.51
1.52
2.53
3.54
4.55
SoapWaterHand SanitizerNone
Num
ber
of
Col
onie
s
Method of Cleaning
ConclusionSources of errorImprovementsExtensionsApplications
Special thanks to…Mrs. PietrangeloMy parentsAll my volunteers
BibliographyBiotechnology: Changing Life Through Science 3-Volume Set. 1 ed. Detroit: UXL, 2007. Print. "Hand Sanitizers - P2RxWiki." ISTC. N.p., n.d. Web. 6 Oct. 2010. <http://lib.wmrc.
uiuc.edu/p2rx-wiki/index.php/Hand_Sanitizers>. "A Chemical Engineer's Guide to Cleaning Just About Anything." Illumin. N.p., n.d. Web. 6
Oct. 2010. "Prokaryotes." The Biology Web. N.p., n.d. Web. 6 Oct. 2010. <http://faculty.clintoncc.
suny.edu/faculty/michael.gregory/files/Bio%20102/Bio 20102 20Laboratory/Prokaryotes/Procaryotes.htm>.
"All About Agar." Science Fair Project Ideas, Answers, & Tools. N.p., n.d. Web. 6 Oct. 2010. <http://www.sciencebuddies.org/science-fair-projects/project_ideas/MicroBio_Agar.shtml>.
"Measuring Bacterial Growth." NEWTON/ANL Home Page. N.p., n.d. Web. 6 Oct. 2010. <http://www.newton.dep.anl.gov/askasci/mole00/mole00353.htm>.
Levy, Foster, Scott A. Reynolds, and Elaine S. Walker. "Hand sanitizer alert." Journal of Environmental Health 69.4 (2006): 48+. Gale Science In Context. Web. 5 Oct. 2010.Human subjects research:
