The Effect of Cold Storage on the Survival of Coccinella undecimpunctata Cary Langley Biology...
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The Effect of Cold Storage on the Survival of Coccinella undecimpunctata Cary Langley Biology Department Eastern CT State University
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Transcript of The Effect of Cold Storage on the Survival of Coccinella undecimpunctata Cary Langley Biology...
The Effect of Cold Storage on the Survival of
Coccinella undecimpunctata
Cary LangleyBiology Department
Eastern CT State University
Possible storage of Coccinella undecimpunctata (Col., Coccinellidae) under low
temperature and its effect on some biological characteristics
A. H. Abdel-Salam and N. F. Abdel-Baky
The Journal of Applied
EntomologyVol. 124: 169-176
Coccinella undecimpunctata
•Common Name = Eleven Spotted Lady Beetle
•Predators of Aphids Whiteflies Weevils
•Biological Control Agents•Cold Stored for Shipping Purposes
Why Study Cold Storage of Coccinella undecimpunctata
• Survival of Cold Temperatures
• Developmental Stages Surviving the Cold
• Developmental Stage Best Used for Short and Long-Term Shipping
Cold Storage Effect
• Egg
• First, Second, Third & Fourth Larval Instars
• Pupae
• Adults: Directly After Eclosion Without Food Five Days After Eclosion With Food Ten Days After Eclosion With Food
• Refrigerated At 6°C
• Refrigerated For 7, 15, 30, 45, 60 Days
Stages Used
Results of the Study
010203040506070
Eggs Hatched (%)
7 15 30 45 60
Storage Periods (Days)
Hatching Success of Refrigerated Eggs
0
20
40
60
80
100
Survival of Larval Instars
(%)
7 15 30 45 60Storage Periods (Days)
1st LarvalInstar
2nd LarvalInstar
3rd LarvalInstar
4th LarvalInstar
Larval Instars Success Rate
0
20
40
60
80
100
Survival of Larval Instars
(%)
7 15 30 45 60Storage Periods (Days)
1st LarvalInstar
2nd LarvalInstar
3rd LarvalInstar
4th LarvalInstar
Larval Instars Success Rate
0
20
40
60
80
100
Survival of Larval Instars
(%)
7 15 30 45 60Storage Periods (Days)
1st LarvalInstar
2nd LarvalInstar
3rd LarvalInstar
4th LarvalInstar
Larval Instars Success Rate
0
10
20
30
40
50
60
70
80
90
100
Survival of Larval Instars
(%)
7 15 30 45 60Storage Periods (Days)
1stLarvalInstar
2ndLarvalInstar
3rdLarvalInstar
4thLarvalInstar
Larval Instars Success Rate
0102030405060708090
Adults Emerged from Stored Pupae (%)
7 15 30 45 60Storage Periods (Days)
Pupae Success Rate
Adults Success Rate
0
10
20
30
40
50
60
70
80
90
100
Survival of Adults (%)
7 15 30 45 60
Storage Periods (Days)
Emergence
5 Days
10 Days
Adults Success Rate
0
10
20
30
40
50
60
70
80
90
100
Survival of Adults (%)
7 15 30 45 60
Storage Periods (Days)
Emergence
5 Days
10 Days
Adults Success Rate
0
10
20
30
40
50
60
70
80
90
100
Survival of Adults (%)
7 15 30 45 60
Storage Periods (Days)
Emergence
5 Days
10 Days
Final Thoughts
• Eggs, Larval Instars & Pupae Survived Short-Term Storage, 7 Days
• Adult Storage More Long-Term, 45 Days Due to Diapause
• Simple Method for Storing Large Numbers• Easy Shipping• Synchronization With Insect Pest• Suppress Insect Pests When Required
In Conclusion
• Cold Stored
• Fed Adults Are Most Receptive
• Biological Control Agents
