Techno-economic study on Stevia production and … · Techno-economic study on Stevia production...
Transcript of Techno-economic study on Stevia production and … · Techno-economic study on Stevia production...
Techno-economic study on Stevia production and development opportunities under Egyptian
conditions
Prof Dr Mostafa MA El-Kholi and Prof Dr Ayman MH Esh
mostafaelkholiyahoocom amp aymaneshgmailcom
Young stevia plant
The sweet herb of Paraguay Stevia rebaudiana Bertoni
produces an alternative sweetener (steviol glycosides) in
its leaves
The advantage is that stevia
sweeteners are
In addition the sweet have functional
and sensory properties superior to
those of many other high potency
sweeteners
The Guarani Indians of Paraguay had known
for centuries about the unique advantages
of kaa he-he (a native term which translates
as sweet herb)
In 1887 Dr Moises Santiago Bertoni
director of the College of Agriculture in
Asuncion first learned of what he
described as this very strange plant
from Indian guides while exploring
Paraguays eastern forests
Dr Moises S BertoniDirector of the College of
Agriculture in Asuncion
Guarani Indians
Bertoni subsequently announced his
discovery of the new species in a botanical
journal published in Asuncion
He wrote one is surprised at the
strange and extreme sweetness
contained therein A fragment of the
leaf only a few square millimeters in
size suffices to keep the mouth sweet
for an hour a few small leaves are
sufficient to sweeten a strong cup of
coffee or tea
The cover page of the
Dictionary of botany
where The first report of
stevia plant
Stevia was first brought to the attention of
the US government in 1918 for the US
Department of Agriculture
In France in 1931 that would later prove
significant There two chemists isolated
the most prevalent of several compounds
that give the stevia leaf its sweet taste a
pure white crystalline extract they named
stevioside
Today the global interests in stevia has
increased and the spread of the stevia
phenomenon will not be limited to a certain
countries
Stevia is cultivated and used in food
elsewhere in East Asia including in China
(since 1984) Korea Taiwan Thailand India
and Malaysia It can also be found in Saint
Kitts and Nevis in parts of South America
(Brazil Colombia Peru Paraguay and
Uruguay) and in Israel China is the worlds
largest exporter of Stevioside
Will sugar always be more advantageous than Kaa-he-e We cannot suppose this The superiority of sugar as an energetic food will not be contested but this does not stop our plant from being stronger as a sweetener
Moises N Bertoni
December 1905 Kaa-he-e Its Nature and Its Properties
Fresh leaves This is the simplest form of Stevia in
Japan they may be purchased loose or in tea bags They
are 15 to 30 times sweeter than sugar
Dried leaves They are 10 to 15 times sweeter than
sugar They have the same uses as fresh leaves and to
extract the Stevioside
Powdered or ground leaves They can be
found in bulk form and in tea bags used as a flavor
enhancer or sweetener in teas salads fruit and coffee
among others
Byproducts (plant remains) The remaining
parts of the plant including stems seeds flowers and even
leaves that were not classified for industrialization are
collected and processed into animal feed or fertilizers
as a mix with silage for animal food or to make a compost
to be use as a fertilizer
Fresh stevia leaves
Dry stevia leaves
Ground stevia leaves
Stevia Liquid Extracts These are
concentrated syrups made from the dried leaves
(dark liquid extracts) or from powdered Steviosides
(clear liquid extracts) in a base of water or alcohol
Used for the sweetening of beverages
Stevia Powders Powdered Stevia extracts
with 40ndash50 percent Sweet glycosides (more than
100 times sweeter than sugar) and Powdered
Stevia extracts with 85ndash97 percent Sweet
glycosides (200-300 times sweeter than sugar)
Stevia liquid extracts
Stevia extracted powders
Weight reduction
Antimicrobial and anti-plaque properties
Non-toxic
Calorie free
Tonic for pancreas
Hypertension
Increase energy levels
Benefits and uses of Stevia
Food usesMaximum use level
(mg Steviol glycosideskg food)
Desserts 500Cold confectionery 500Pickles 1000Sweetcorn 200Biscuits 300Breverages 500Yogurt 500Sauces 1000Delicacies 1000Bread 160
The global market for non-sugar sweeteners in 2011 was at about $92
billion and is expected to reach $93 billion in 2012 The market is expected
to rise at a compound annual growth rate (CAGR) of 11 and reach nearly
$99 billion by 2016 The North American market is estimated to reach
nearly $59 billion in 2012 and The European market represents the second
largest market category with the expected sale of nearly $19 billion in
2012
0
1000
2000
3000
4000
5000
6000
2009 2010 2011 2016
North america Europe Rest of the world
Millio
n U
SD
Sited from httpwwwprlogorg11726488-global-markets-for-non-sugar-sweetenershtml
Since the Stevia extract Rebaudioside A (Reb-A) received
GRAS approval by the US Food and Drug Administration (FDA)
on 17 December 2008 and the approval of the European
Commission on 2 December 2011 there have been several
hundred product launches by food and beverage companies
Steviarsquos status as a global ingredient was secured
with its incorporation into leading beverage
brands manufactured by Coca-Cola and Pepsi
companies in 2009
Now the worldrsquos largest natural
sweetness agent market opened the
door to the Stevia extract of Stevia
extract exports entering the
accelerated growth period
EUROPE9
SOUTH AMERICA
24
NORTH AMERICA
30
ASIA PACIFIC36
OTHERS1
Sited from
httpissuucomzenith_internationaldocsstevia_report_sample
Zenith forecasts that the global market for stevia will reach 11000 metric
tons by 2014 equivalent to US$825 million by value
0
05
1
15
2
25
3
35
2006 2007 2008 2009 2010
(X 1000 ton)
Sited from httpissuucomzenith_internationaldocsstevia_report_sample
One Feddan of stevia produce 4 to 6 tonsyear
(3 harvests) of Dry Leaves
The International price of dry leaves (ge 10
stevisoid) range from 2 to 4 kg USD
Stevia harvest 3 times a year under Egyptian
conditions
International price of 97 stevasoid range from
100 to 150 USDkg
Stevia stay in the field for several years (3 to 5)
years
YEAR 1 YEAR 2 YEAR 3 YEAR 4 TOTAL
Total costsyear (USD) 3061 2611 2611 2611 10896
AVERAGE PRODUCTION (TON)YEAR 45 45 45 45 18
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 9000 9000 9000 9000 36000
NET PROFIT 5938 6388 6388 6388 25103
OF PROFIT 1939 2446 2446 2446 2303
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 3 3 3 3 12
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 6000 6000 6000 6000 24000
NET PROFIT 2938333 3388333 3388333 3388333 1310333
OF PROFIT 959 1297 1297 1297 1202
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 2 2 2 2 8
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME (USD) 4000 4000 4000 4000 16000
NET PROFIT (USD) 9383333 1388333 1388333 1388333 5103333
OF PROFIT 306 531 531 531 468
From the current study we can notice that the major
costs are the field rent (5000 LE = 833$) and the
starter transplants (8000 LE = 1333$) From this
point to reduce the production costs it is suggested
to focus on producing stevia in the new reclaimed
lands which will lead to reduce the rent cost from
30 to 50 Also a system for producing stevia
seedlings (nurseries) to use as a starter transplants
should be introduced to the producers as a way to
reduce the starter material costs more than 50
One more advantage of cultivating stevia in new
reclaimed lands is the easy use of mechanization
and for setting up modified irrigation systems and
agriculture practices suitable for stevia
bull Stevia is an obligate short day plant with a critical day length of about 11-13 h
bull Long‐days delay flowering and increase stevasoid accumulation Extensive variability within populations for day length sensitivity has been reported
Stevia belongs to
Class Magnoliopsida (Dicotyledons)
Order Asterales
Family Asteraceae or Compositae
Stevia rebaudiana has many commonly
known Synonyms Sweet herb sweet
leaf sugar leaf and honey leaf or simply
Stevia
(مثل عباد الشمس) العائله المركبه
(و زيادة فترات النهار تؤخر االزهار و تسيد من ترجمع االستيفاسىيد( ساعه نهار 13-11)من نباتات النهار القصير
bull The RootThe root of stevia plant is fibrous filamentary
and perennial abundantly branched and
strain distributed near the surface and is
the only organ of the plant that does not
contain stevioside Stem
Root
Foliage
جذور نبات االستيفيا ليفيه فتيلية والمعمرة متشعبه و موزعه بالقرب من سطح
التربه وهي الجهاز الوحيد من النبات الذي ال يحتوي علي مادة االستيفاسويد
المحليه
bull The stemThe stem is annual sub-woody more or less
pubescent with a tendency to lean is more
or less branched During early development
does not have branches becoming
multicaule after the first growing season
coming to produce up to 20 stems in 3 to 4
years Under optimal conditions the stem
can reach five feet in height
Stem
Root
Foliage
bull The leafThe leaves are oval or elliptic lanceolate
small simple toothed edge or margin
sometimes in whorls somewhat hairy
The leaf is the organ with the highest
content of sweetener
Stem
Root
Foliage
ورقه بسيطة بيضاويه صغيره مسننه
bull The FlowersStevia classified as a short-day plant
placing the critical photoperiod at
12 to 13 hours depending on the
ecotype
Flowers are hermaphrodite
small whitish in terminal or axillary short
chapters grouped in corymbose panicles
االزهار صغيره خنثي بيضاء في مجاميع
The hermaphrodite flower is small whitish in
terminal or axillary short chapters grouped in
corymbose panicles A plant takes more than
a month to produce all their flowers The
plant is self-incompatible sporophytic type
and classified as obligatory
apomictic Pollination is by insects
Compound name R1 R2
Stevioside β-Glc β-Glc-β-Glc(2rarr1)
RebaudiosideA β-Glc β-Glc-β-Glc
I
β-Glc (3rarr1)
Rebaudioside C β-Glcβ-Glc -α-Rha(2rarr1)
I
β-Glc (3rarr 1)
Dulcoside A β-Glc β-Glc - α -Rha(2rarrl)
Rubusoside β-Glc β-Glc
Steviolbioside H β-Glc - β-Glc (2rarr1)
RebaudiosideB H β-Glc - β-Glc (2rarr1)
I
β-Glc (3rarr1)
Steviol (RI = R2 = H) is the aglycone of the steviol glycosides Glc and Rha
represent respectively glucose and rhamnose sugar moieties
The seven specific steviol glycosides
The component glycosides
of particular interest for
stevia leaf sweetening
property are stevioside and
Reb A
Powdered Stevia extracts
with 85ndash97 percent Sweet
glycosides (200-300 times
sweeter than sugar
(Sucrose)
Always Keep In
Mind We are
cultivating Stevia
for its leaves
bull A variety of soil types ranging from course
textured sands to well drained loamsare suitable
for growing stevia
but not heavy clay or poorly drained sites
bull Stevia requires very good drainage system
bull Stevia can be grown on a wide range of soil with
pH range from 6 up to 9
bull soil should not be saline
bull Soils with very heavy clay content or high pH
should also be treated or avoided
Before planting each and every
soil sample should be properly
tested and analyzed by an expert
Soil type
bull Climate bull The plant is climatically suited to climates from
temperate to sub-tropical where temperatures
range between 21-43degC with an average of
24degC
bull During the growing season it
seems to thrive in a temperature
range of 15 degC to 34degC provided all
input resources and good
management practices are
incorporated
bull Plant Varieties
bull There are many of Stevia rebaudiana
varieties developed all around the world
These varieties have been developed
for different climate requirements and
Stevioside and rebaudioside content
bull The minimum market requirement of
Stevioside and rebaudioside is 9
bull The grower should select proper
varieties with adequate guarantees from
the planting material suppliers about
minimum assured stevioside and
rebaudioside contents
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
Young stevia plant
The sweet herb of Paraguay Stevia rebaudiana Bertoni
produces an alternative sweetener (steviol glycosides) in
its leaves
The advantage is that stevia
sweeteners are
In addition the sweet have functional
and sensory properties superior to
those of many other high potency
sweeteners
The Guarani Indians of Paraguay had known
for centuries about the unique advantages
of kaa he-he (a native term which translates
as sweet herb)
In 1887 Dr Moises Santiago Bertoni
director of the College of Agriculture in
Asuncion first learned of what he
described as this very strange plant
from Indian guides while exploring
Paraguays eastern forests
Dr Moises S BertoniDirector of the College of
Agriculture in Asuncion
Guarani Indians
Bertoni subsequently announced his
discovery of the new species in a botanical
journal published in Asuncion
He wrote one is surprised at the
strange and extreme sweetness
contained therein A fragment of the
leaf only a few square millimeters in
size suffices to keep the mouth sweet
for an hour a few small leaves are
sufficient to sweeten a strong cup of
coffee or tea
The cover page of the
Dictionary of botany
where The first report of
stevia plant
Stevia was first brought to the attention of
the US government in 1918 for the US
Department of Agriculture
In France in 1931 that would later prove
significant There two chemists isolated
the most prevalent of several compounds
that give the stevia leaf its sweet taste a
pure white crystalline extract they named
stevioside
Today the global interests in stevia has
increased and the spread of the stevia
phenomenon will not be limited to a certain
countries
Stevia is cultivated and used in food
elsewhere in East Asia including in China
(since 1984) Korea Taiwan Thailand India
and Malaysia It can also be found in Saint
Kitts and Nevis in parts of South America
(Brazil Colombia Peru Paraguay and
Uruguay) and in Israel China is the worlds
largest exporter of Stevioside
Will sugar always be more advantageous than Kaa-he-e We cannot suppose this The superiority of sugar as an energetic food will not be contested but this does not stop our plant from being stronger as a sweetener
Moises N Bertoni
December 1905 Kaa-he-e Its Nature and Its Properties
Fresh leaves This is the simplest form of Stevia in
Japan they may be purchased loose or in tea bags They
are 15 to 30 times sweeter than sugar
Dried leaves They are 10 to 15 times sweeter than
sugar They have the same uses as fresh leaves and to
extract the Stevioside
Powdered or ground leaves They can be
found in bulk form and in tea bags used as a flavor
enhancer or sweetener in teas salads fruit and coffee
among others
Byproducts (plant remains) The remaining
parts of the plant including stems seeds flowers and even
leaves that were not classified for industrialization are
collected and processed into animal feed or fertilizers
as a mix with silage for animal food or to make a compost
to be use as a fertilizer
Fresh stevia leaves
Dry stevia leaves
Ground stevia leaves
Stevia Liquid Extracts These are
concentrated syrups made from the dried leaves
(dark liquid extracts) or from powdered Steviosides
(clear liquid extracts) in a base of water or alcohol
Used for the sweetening of beverages
Stevia Powders Powdered Stevia extracts
with 40ndash50 percent Sweet glycosides (more than
100 times sweeter than sugar) and Powdered
Stevia extracts with 85ndash97 percent Sweet
glycosides (200-300 times sweeter than sugar)
Stevia liquid extracts
Stevia extracted powders
Weight reduction
Antimicrobial and anti-plaque properties
Non-toxic
Calorie free
Tonic for pancreas
Hypertension
Increase energy levels
Benefits and uses of Stevia
Food usesMaximum use level
(mg Steviol glycosideskg food)
Desserts 500Cold confectionery 500Pickles 1000Sweetcorn 200Biscuits 300Breverages 500Yogurt 500Sauces 1000Delicacies 1000Bread 160
The global market for non-sugar sweeteners in 2011 was at about $92
billion and is expected to reach $93 billion in 2012 The market is expected
to rise at a compound annual growth rate (CAGR) of 11 and reach nearly
$99 billion by 2016 The North American market is estimated to reach
nearly $59 billion in 2012 and The European market represents the second
largest market category with the expected sale of nearly $19 billion in
2012
0
1000
2000
3000
4000
5000
6000
2009 2010 2011 2016
North america Europe Rest of the world
Millio
n U
SD
Sited from httpwwwprlogorg11726488-global-markets-for-non-sugar-sweetenershtml
Since the Stevia extract Rebaudioside A (Reb-A) received
GRAS approval by the US Food and Drug Administration (FDA)
on 17 December 2008 and the approval of the European
Commission on 2 December 2011 there have been several
hundred product launches by food and beverage companies
Steviarsquos status as a global ingredient was secured
with its incorporation into leading beverage
brands manufactured by Coca-Cola and Pepsi
companies in 2009
Now the worldrsquos largest natural
sweetness agent market opened the
door to the Stevia extract of Stevia
extract exports entering the
accelerated growth period
EUROPE9
SOUTH AMERICA
24
NORTH AMERICA
30
ASIA PACIFIC36
OTHERS1
Sited from
httpissuucomzenith_internationaldocsstevia_report_sample
Zenith forecasts that the global market for stevia will reach 11000 metric
tons by 2014 equivalent to US$825 million by value
0
05
1
15
2
25
3
35
2006 2007 2008 2009 2010
(X 1000 ton)
Sited from httpissuucomzenith_internationaldocsstevia_report_sample
One Feddan of stevia produce 4 to 6 tonsyear
(3 harvests) of Dry Leaves
The International price of dry leaves (ge 10
stevisoid) range from 2 to 4 kg USD
Stevia harvest 3 times a year under Egyptian
conditions
International price of 97 stevasoid range from
100 to 150 USDkg
Stevia stay in the field for several years (3 to 5)
years
YEAR 1 YEAR 2 YEAR 3 YEAR 4 TOTAL
Total costsyear (USD) 3061 2611 2611 2611 10896
AVERAGE PRODUCTION (TON)YEAR 45 45 45 45 18
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 9000 9000 9000 9000 36000
NET PROFIT 5938 6388 6388 6388 25103
OF PROFIT 1939 2446 2446 2446 2303
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 3 3 3 3 12
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 6000 6000 6000 6000 24000
NET PROFIT 2938333 3388333 3388333 3388333 1310333
OF PROFIT 959 1297 1297 1297 1202
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 2 2 2 2 8
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME (USD) 4000 4000 4000 4000 16000
NET PROFIT (USD) 9383333 1388333 1388333 1388333 5103333
OF PROFIT 306 531 531 531 468
From the current study we can notice that the major
costs are the field rent (5000 LE = 833$) and the
starter transplants (8000 LE = 1333$) From this
point to reduce the production costs it is suggested
to focus on producing stevia in the new reclaimed
lands which will lead to reduce the rent cost from
30 to 50 Also a system for producing stevia
seedlings (nurseries) to use as a starter transplants
should be introduced to the producers as a way to
reduce the starter material costs more than 50
One more advantage of cultivating stevia in new
reclaimed lands is the easy use of mechanization
and for setting up modified irrigation systems and
agriculture practices suitable for stevia
bull Stevia is an obligate short day plant with a critical day length of about 11-13 h
bull Long‐days delay flowering and increase stevasoid accumulation Extensive variability within populations for day length sensitivity has been reported
Stevia belongs to
Class Magnoliopsida (Dicotyledons)
Order Asterales
Family Asteraceae or Compositae
Stevia rebaudiana has many commonly
known Synonyms Sweet herb sweet
leaf sugar leaf and honey leaf or simply
Stevia
(مثل عباد الشمس) العائله المركبه
(و زيادة فترات النهار تؤخر االزهار و تسيد من ترجمع االستيفاسىيد( ساعه نهار 13-11)من نباتات النهار القصير
bull The RootThe root of stevia plant is fibrous filamentary
and perennial abundantly branched and
strain distributed near the surface and is
the only organ of the plant that does not
contain stevioside Stem
Root
Foliage
جذور نبات االستيفيا ليفيه فتيلية والمعمرة متشعبه و موزعه بالقرب من سطح
التربه وهي الجهاز الوحيد من النبات الذي ال يحتوي علي مادة االستيفاسويد
المحليه
bull The stemThe stem is annual sub-woody more or less
pubescent with a tendency to lean is more
or less branched During early development
does not have branches becoming
multicaule after the first growing season
coming to produce up to 20 stems in 3 to 4
years Under optimal conditions the stem
can reach five feet in height
Stem
Root
Foliage
bull The leafThe leaves are oval or elliptic lanceolate
small simple toothed edge or margin
sometimes in whorls somewhat hairy
The leaf is the organ with the highest
content of sweetener
Stem
Root
Foliage
ورقه بسيطة بيضاويه صغيره مسننه
bull The FlowersStevia classified as a short-day plant
placing the critical photoperiod at
12 to 13 hours depending on the
ecotype
Flowers are hermaphrodite
small whitish in terminal or axillary short
chapters grouped in corymbose panicles
االزهار صغيره خنثي بيضاء في مجاميع
The hermaphrodite flower is small whitish in
terminal or axillary short chapters grouped in
corymbose panicles A plant takes more than
a month to produce all their flowers The
plant is self-incompatible sporophytic type
and classified as obligatory
apomictic Pollination is by insects
Compound name R1 R2
Stevioside β-Glc β-Glc-β-Glc(2rarr1)
RebaudiosideA β-Glc β-Glc-β-Glc
I
β-Glc (3rarr1)
Rebaudioside C β-Glcβ-Glc -α-Rha(2rarr1)
I
β-Glc (3rarr 1)
Dulcoside A β-Glc β-Glc - α -Rha(2rarrl)
Rubusoside β-Glc β-Glc
Steviolbioside H β-Glc - β-Glc (2rarr1)
RebaudiosideB H β-Glc - β-Glc (2rarr1)
I
β-Glc (3rarr1)
Steviol (RI = R2 = H) is the aglycone of the steviol glycosides Glc and Rha
represent respectively glucose and rhamnose sugar moieties
The seven specific steviol glycosides
The component glycosides
of particular interest for
stevia leaf sweetening
property are stevioside and
Reb A
Powdered Stevia extracts
with 85ndash97 percent Sweet
glycosides (200-300 times
sweeter than sugar
(Sucrose)
Always Keep In
Mind We are
cultivating Stevia
for its leaves
bull A variety of soil types ranging from course
textured sands to well drained loamsare suitable
for growing stevia
but not heavy clay or poorly drained sites
bull Stevia requires very good drainage system
bull Stevia can be grown on a wide range of soil with
pH range from 6 up to 9
bull soil should not be saline
bull Soils with very heavy clay content or high pH
should also be treated or avoided
Before planting each and every
soil sample should be properly
tested and analyzed by an expert
Soil type
bull Climate bull The plant is climatically suited to climates from
temperate to sub-tropical where temperatures
range between 21-43degC with an average of
24degC
bull During the growing season it
seems to thrive in a temperature
range of 15 degC to 34degC provided all
input resources and good
management practices are
incorporated
bull Plant Varieties
bull There are many of Stevia rebaudiana
varieties developed all around the world
These varieties have been developed
for different climate requirements and
Stevioside and rebaudioside content
bull The minimum market requirement of
Stevioside and rebaudioside is 9
bull The grower should select proper
varieties with adequate guarantees from
the planting material suppliers about
minimum assured stevioside and
rebaudioside contents
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
The Guarani Indians of Paraguay had known
for centuries about the unique advantages
of kaa he-he (a native term which translates
as sweet herb)
In 1887 Dr Moises Santiago Bertoni
director of the College of Agriculture in
Asuncion first learned of what he
described as this very strange plant
from Indian guides while exploring
Paraguays eastern forests
Dr Moises S BertoniDirector of the College of
Agriculture in Asuncion
Guarani Indians
Bertoni subsequently announced his
discovery of the new species in a botanical
journal published in Asuncion
He wrote one is surprised at the
strange and extreme sweetness
contained therein A fragment of the
leaf only a few square millimeters in
size suffices to keep the mouth sweet
for an hour a few small leaves are
sufficient to sweeten a strong cup of
coffee or tea
The cover page of the
Dictionary of botany
where The first report of
stevia plant
Stevia was first brought to the attention of
the US government in 1918 for the US
Department of Agriculture
In France in 1931 that would later prove
significant There two chemists isolated
the most prevalent of several compounds
that give the stevia leaf its sweet taste a
pure white crystalline extract they named
stevioside
Today the global interests in stevia has
increased and the spread of the stevia
phenomenon will not be limited to a certain
countries
Stevia is cultivated and used in food
elsewhere in East Asia including in China
(since 1984) Korea Taiwan Thailand India
and Malaysia It can also be found in Saint
Kitts and Nevis in parts of South America
(Brazil Colombia Peru Paraguay and
Uruguay) and in Israel China is the worlds
largest exporter of Stevioside
Will sugar always be more advantageous than Kaa-he-e We cannot suppose this The superiority of sugar as an energetic food will not be contested but this does not stop our plant from being stronger as a sweetener
Moises N Bertoni
December 1905 Kaa-he-e Its Nature and Its Properties
Fresh leaves This is the simplest form of Stevia in
Japan they may be purchased loose or in tea bags They
are 15 to 30 times sweeter than sugar
Dried leaves They are 10 to 15 times sweeter than
sugar They have the same uses as fresh leaves and to
extract the Stevioside
Powdered or ground leaves They can be
found in bulk form and in tea bags used as a flavor
enhancer or sweetener in teas salads fruit and coffee
among others
Byproducts (plant remains) The remaining
parts of the plant including stems seeds flowers and even
leaves that were not classified for industrialization are
collected and processed into animal feed or fertilizers
as a mix with silage for animal food or to make a compost
to be use as a fertilizer
Fresh stevia leaves
Dry stevia leaves
Ground stevia leaves
Stevia Liquid Extracts These are
concentrated syrups made from the dried leaves
(dark liquid extracts) or from powdered Steviosides
(clear liquid extracts) in a base of water or alcohol
Used for the sweetening of beverages
Stevia Powders Powdered Stevia extracts
with 40ndash50 percent Sweet glycosides (more than
100 times sweeter than sugar) and Powdered
Stevia extracts with 85ndash97 percent Sweet
glycosides (200-300 times sweeter than sugar)
Stevia liquid extracts
Stevia extracted powders
Weight reduction
Antimicrobial and anti-plaque properties
Non-toxic
Calorie free
Tonic for pancreas
Hypertension
Increase energy levels
Benefits and uses of Stevia
Food usesMaximum use level
(mg Steviol glycosideskg food)
Desserts 500Cold confectionery 500Pickles 1000Sweetcorn 200Biscuits 300Breverages 500Yogurt 500Sauces 1000Delicacies 1000Bread 160
The global market for non-sugar sweeteners in 2011 was at about $92
billion and is expected to reach $93 billion in 2012 The market is expected
to rise at a compound annual growth rate (CAGR) of 11 and reach nearly
$99 billion by 2016 The North American market is estimated to reach
nearly $59 billion in 2012 and The European market represents the second
largest market category with the expected sale of nearly $19 billion in
2012
0
1000
2000
3000
4000
5000
6000
2009 2010 2011 2016
North america Europe Rest of the world
Millio
n U
SD
Sited from httpwwwprlogorg11726488-global-markets-for-non-sugar-sweetenershtml
Since the Stevia extract Rebaudioside A (Reb-A) received
GRAS approval by the US Food and Drug Administration (FDA)
on 17 December 2008 and the approval of the European
Commission on 2 December 2011 there have been several
hundred product launches by food and beverage companies
Steviarsquos status as a global ingredient was secured
with its incorporation into leading beverage
brands manufactured by Coca-Cola and Pepsi
companies in 2009
Now the worldrsquos largest natural
sweetness agent market opened the
door to the Stevia extract of Stevia
extract exports entering the
accelerated growth period
EUROPE9
SOUTH AMERICA
24
NORTH AMERICA
30
ASIA PACIFIC36
OTHERS1
Sited from
httpissuucomzenith_internationaldocsstevia_report_sample
Zenith forecasts that the global market for stevia will reach 11000 metric
tons by 2014 equivalent to US$825 million by value
0
05
1
15
2
25
3
35
2006 2007 2008 2009 2010
(X 1000 ton)
Sited from httpissuucomzenith_internationaldocsstevia_report_sample
One Feddan of stevia produce 4 to 6 tonsyear
(3 harvests) of Dry Leaves
The International price of dry leaves (ge 10
stevisoid) range from 2 to 4 kg USD
Stevia harvest 3 times a year under Egyptian
conditions
International price of 97 stevasoid range from
100 to 150 USDkg
Stevia stay in the field for several years (3 to 5)
years
YEAR 1 YEAR 2 YEAR 3 YEAR 4 TOTAL
Total costsyear (USD) 3061 2611 2611 2611 10896
AVERAGE PRODUCTION (TON)YEAR 45 45 45 45 18
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 9000 9000 9000 9000 36000
NET PROFIT 5938 6388 6388 6388 25103
OF PROFIT 1939 2446 2446 2446 2303
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 3 3 3 3 12
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 6000 6000 6000 6000 24000
NET PROFIT 2938333 3388333 3388333 3388333 1310333
OF PROFIT 959 1297 1297 1297 1202
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 2 2 2 2 8
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME (USD) 4000 4000 4000 4000 16000
NET PROFIT (USD) 9383333 1388333 1388333 1388333 5103333
OF PROFIT 306 531 531 531 468
From the current study we can notice that the major
costs are the field rent (5000 LE = 833$) and the
starter transplants (8000 LE = 1333$) From this
point to reduce the production costs it is suggested
to focus on producing stevia in the new reclaimed
lands which will lead to reduce the rent cost from
30 to 50 Also a system for producing stevia
seedlings (nurseries) to use as a starter transplants
should be introduced to the producers as a way to
reduce the starter material costs more than 50
One more advantage of cultivating stevia in new
reclaimed lands is the easy use of mechanization
and for setting up modified irrigation systems and
agriculture practices suitable for stevia
bull Stevia is an obligate short day plant with a critical day length of about 11-13 h
bull Long‐days delay flowering and increase stevasoid accumulation Extensive variability within populations for day length sensitivity has been reported
Stevia belongs to
Class Magnoliopsida (Dicotyledons)
Order Asterales
Family Asteraceae or Compositae
Stevia rebaudiana has many commonly
known Synonyms Sweet herb sweet
leaf sugar leaf and honey leaf or simply
Stevia
(مثل عباد الشمس) العائله المركبه
(و زيادة فترات النهار تؤخر االزهار و تسيد من ترجمع االستيفاسىيد( ساعه نهار 13-11)من نباتات النهار القصير
bull The RootThe root of stevia plant is fibrous filamentary
and perennial abundantly branched and
strain distributed near the surface and is
the only organ of the plant that does not
contain stevioside Stem
Root
Foliage
جذور نبات االستيفيا ليفيه فتيلية والمعمرة متشعبه و موزعه بالقرب من سطح
التربه وهي الجهاز الوحيد من النبات الذي ال يحتوي علي مادة االستيفاسويد
المحليه
bull The stemThe stem is annual sub-woody more or less
pubescent with a tendency to lean is more
or less branched During early development
does not have branches becoming
multicaule after the first growing season
coming to produce up to 20 stems in 3 to 4
years Under optimal conditions the stem
can reach five feet in height
Stem
Root
Foliage
bull The leafThe leaves are oval or elliptic lanceolate
small simple toothed edge or margin
sometimes in whorls somewhat hairy
The leaf is the organ with the highest
content of sweetener
Stem
Root
Foliage
ورقه بسيطة بيضاويه صغيره مسننه
bull The FlowersStevia classified as a short-day plant
placing the critical photoperiod at
12 to 13 hours depending on the
ecotype
Flowers are hermaphrodite
small whitish in terminal or axillary short
chapters grouped in corymbose panicles
االزهار صغيره خنثي بيضاء في مجاميع
The hermaphrodite flower is small whitish in
terminal or axillary short chapters grouped in
corymbose panicles A plant takes more than
a month to produce all their flowers The
plant is self-incompatible sporophytic type
and classified as obligatory
apomictic Pollination is by insects
Compound name R1 R2
Stevioside β-Glc β-Glc-β-Glc(2rarr1)
RebaudiosideA β-Glc β-Glc-β-Glc
I
β-Glc (3rarr1)
Rebaudioside C β-Glcβ-Glc -α-Rha(2rarr1)
I
β-Glc (3rarr 1)
Dulcoside A β-Glc β-Glc - α -Rha(2rarrl)
Rubusoside β-Glc β-Glc
Steviolbioside H β-Glc - β-Glc (2rarr1)
RebaudiosideB H β-Glc - β-Glc (2rarr1)
I
β-Glc (3rarr1)
Steviol (RI = R2 = H) is the aglycone of the steviol glycosides Glc and Rha
represent respectively glucose and rhamnose sugar moieties
The seven specific steviol glycosides
The component glycosides
of particular interest for
stevia leaf sweetening
property are stevioside and
Reb A
Powdered Stevia extracts
with 85ndash97 percent Sweet
glycosides (200-300 times
sweeter than sugar
(Sucrose)
Always Keep In
Mind We are
cultivating Stevia
for its leaves
bull A variety of soil types ranging from course
textured sands to well drained loamsare suitable
for growing stevia
but not heavy clay or poorly drained sites
bull Stevia requires very good drainage system
bull Stevia can be grown on a wide range of soil with
pH range from 6 up to 9
bull soil should not be saline
bull Soils with very heavy clay content or high pH
should also be treated or avoided
Before planting each and every
soil sample should be properly
tested and analyzed by an expert
Soil type
bull Climate bull The plant is climatically suited to climates from
temperate to sub-tropical where temperatures
range between 21-43degC with an average of
24degC
bull During the growing season it
seems to thrive in a temperature
range of 15 degC to 34degC provided all
input resources and good
management practices are
incorporated
bull Plant Varieties
bull There are many of Stevia rebaudiana
varieties developed all around the world
These varieties have been developed
for different climate requirements and
Stevioside and rebaudioside content
bull The minimum market requirement of
Stevioside and rebaudioside is 9
bull The grower should select proper
varieties with adequate guarantees from
the planting material suppliers about
minimum assured stevioside and
rebaudioside contents
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
Bertoni subsequently announced his
discovery of the new species in a botanical
journal published in Asuncion
He wrote one is surprised at the
strange and extreme sweetness
contained therein A fragment of the
leaf only a few square millimeters in
size suffices to keep the mouth sweet
for an hour a few small leaves are
sufficient to sweeten a strong cup of
coffee or tea
The cover page of the
Dictionary of botany
where The first report of
stevia plant
Stevia was first brought to the attention of
the US government in 1918 for the US
Department of Agriculture
In France in 1931 that would later prove
significant There two chemists isolated
the most prevalent of several compounds
that give the stevia leaf its sweet taste a
pure white crystalline extract they named
stevioside
Today the global interests in stevia has
increased and the spread of the stevia
phenomenon will not be limited to a certain
countries
Stevia is cultivated and used in food
elsewhere in East Asia including in China
(since 1984) Korea Taiwan Thailand India
and Malaysia It can also be found in Saint
Kitts and Nevis in parts of South America
(Brazil Colombia Peru Paraguay and
Uruguay) and in Israel China is the worlds
largest exporter of Stevioside
Will sugar always be more advantageous than Kaa-he-e We cannot suppose this The superiority of sugar as an energetic food will not be contested but this does not stop our plant from being stronger as a sweetener
Moises N Bertoni
December 1905 Kaa-he-e Its Nature and Its Properties
Fresh leaves This is the simplest form of Stevia in
Japan they may be purchased loose or in tea bags They
are 15 to 30 times sweeter than sugar
Dried leaves They are 10 to 15 times sweeter than
sugar They have the same uses as fresh leaves and to
extract the Stevioside
Powdered or ground leaves They can be
found in bulk form and in tea bags used as a flavor
enhancer or sweetener in teas salads fruit and coffee
among others
Byproducts (plant remains) The remaining
parts of the plant including stems seeds flowers and even
leaves that were not classified for industrialization are
collected and processed into animal feed or fertilizers
as a mix with silage for animal food or to make a compost
to be use as a fertilizer
Fresh stevia leaves
Dry stevia leaves
Ground stevia leaves
Stevia Liquid Extracts These are
concentrated syrups made from the dried leaves
(dark liquid extracts) or from powdered Steviosides
(clear liquid extracts) in a base of water or alcohol
Used for the sweetening of beverages
Stevia Powders Powdered Stevia extracts
with 40ndash50 percent Sweet glycosides (more than
100 times sweeter than sugar) and Powdered
Stevia extracts with 85ndash97 percent Sweet
glycosides (200-300 times sweeter than sugar)
Stevia liquid extracts
Stevia extracted powders
Weight reduction
Antimicrobial and anti-plaque properties
Non-toxic
Calorie free
Tonic for pancreas
Hypertension
Increase energy levels
Benefits and uses of Stevia
Food usesMaximum use level
(mg Steviol glycosideskg food)
Desserts 500Cold confectionery 500Pickles 1000Sweetcorn 200Biscuits 300Breverages 500Yogurt 500Sauces 1000Delicacies 1000Bread 160
The global market for non-sugar sweeteners in 2011 was at about $92
billion and is expected to reach $93 billion in 2012 The market is expected
to rise at a compound annual growth rate (CAGR) of 11 and reach nearly
$99 billion by 2016 The North American market is estimated to reach
nearly $59 billion in 2012 and The European market represents the second
largest market category with the expected sale of nearly $19 billion in
2012
0
1000
2000
3000
4000
5000
6000
2009 2010 2011 2016
North america Europe Rest of the world
Millio
n U
SD
Sited from httpwwwprlogorg11726488-global-markets-for-non-sugar-sweetenershtml
Since the Stevia extract Rebaudioside A (Reb-A) received
GRAS approval by the US Food and Drug Administration (FDA)
on 17 December 2008 and the approval of the European
Commission on 2 December 2011 there have been several
hundred product launches by food and beverage companies
Steviarsquos status as a global ingredient was secured
with its incorporation into leading beverage
brands manufactured by Coca-Cola and Pepsi
companies in 2009
Now the worldrsquos largest natural
sweetness agent market opened the
door to the Stevia extract of Stevia
extract exports entering the
accelerated growth period
EUROPE9
SOUTH AMERICA
24
NORTH AMERICA
30
ASIA PACIFIC36
OTHERS1
Sited from
httpissuucomzenith_internationaldocsstevia_report_sample
Zenith forecasts that the global market for stevia will reach 11000 metric
tons by 2014 equivalent to US$825 million by value
0
05
1
15
2
25
3
35
2006 2007 2008 2009 2010
(X 1000 ton)
Sited from httpissuucomzenith_internationaldocsstevia_report_sample
One Feddan of stevia produce 4 to 6 tonsyear
(3 harvests) of Dry Leaves
The International price of dry leaves (ge 10
stevisoid) range from 2 to 4 kg USD
Stevia harvest 3 times a year under Egyptian
conditions
International price of 97 stevasoid range from
100 to 150 USDkg
Stevia stay in the field for several years (3 to 5)
years
YEAR 1 YEAR 2 YEAR 3 YEAR 4 TOTAL
Total costsyear (USD) 3061 2611 2611 2611 10896
AVERAGE PRODUCTION (TON)YEAR 45 45 45 45 18
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 9000 9000 9000 9000 36000
NET PROFIT 5938 6388 6388 6388 25103
OF PROFIT 1939 2446 2446 2446 2303
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 3 3 3 3 12
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 6000 6000 6000 6000 24000
NET PROFIT 2938333 3388333 3388333 3388333 1310333
OF PROFIT 959 1297 1297 1297 1202
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 2 2 2 2 8
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME (USD) 4000 4000 4000 4000 16000
NET PROFIT (USD) 9383333 1388333 1388333 1388333 5103333
OF PROFIT 306 531 531 531 468
From the current study we can notice that the major
costs are the field rent (5000 LE = 833$) and the
starter transplants (8000 LE = 1333$) From this
point to reduce the production costs it is suggested
to focus on producing stevia in the new reclaimed
lands which will lead to reduce the rent cost from
30 to 50 Also a system for producing stevia
seedlings (nurseries) to use as a starter transplants
should be introduced to the producers as a way to
reduce the starter material costs more than 50
One more advantage of cultivating stevia in new
reclaimed lands is the easy use of mechanization
and for setting up modified irrigation systems and
agriculture practices suitable for stevia
bull Stevia is an obligate short day plant with a critical day length of about 11-13 h
bull Long‐days delay flowering and increase stevasoid accumulation Extensive variability within populations for day length sensitivity has been reported
Stevia belongs to
Class Magnoliopsida (Dicotyledons)
Order Asterales
Family Asteraceae or Compositae
Stevia rebaudiana has many commonly
known Synonyms Sweet herb sweet
leaf sugar leaf and honey leaf or simply
Stevia
(مثل عباد الشمس) العائله المركبه
(و زيادة فترات النهار تؤخر االزهار و تسيد من ترجمع االستيفاسىيد( ساعه نهار 13-11)من نباتات النهار القصير
bull The RootThe root of stevia plant is fibrous filamentary
and perennial abundantly branched and
strain distributed near the surface and is
the only organ of the plant that does not
contain stevioside Stem
Root
Foliage
جذور نبات االستيفيا ليفيه فتيلية والمعمرة متشعبه و موزعه بالقرب من سطح
التربه وهي الجهاز الوحيد من النبات الذي ال يحتوي علي مادة االستيفاسويد
المحليه
bull The stemThe stem is annual sub-woody more or less
pubescent with a tendency to lean is more
or less branched During early development
does not have branches becoming
multicaule after the first growing season
coming to produce up to 20 stems in 3 to 4
years Under optimal conditions the stem
can reach five feet in height
Stem
Root
Foliage
bull The leafThe leaves are oval or elliptic lanceolate
small simple toothed edge or margin
sometimes in whorls somewhat hairy
The leaf is the organ with the highest
content of sweetener
Stem
Root
Foliage
ورقه بسيطة بيضاويه صغيره مسننه
bull The FlowersStevia classified as a short-day plant
placing the critical photoperiod at
12 to 13 hours depending on the
ecotype
Flowers are hermaphrodite
small whitish in terminal or axillary short
chapters grouped in corymbose panicles
االزهار صغيره خنثي بيضاء في مجاميع
The hermaphrodite flower is small whitish in
terminal or axillary short chapters grouped in
corymbose panicles A plant takes more than
a month to produce all their flowers The
plant is self-incompatible sporophytic type
and classified as obligatory
apomictic Pollination is by insects
Compound name R1 R2
Stevioside β-Glc β-Glc-β-Glc(2rarr1)
RebaudiosideA β-Glc β-Glc-β-Glc
I
β-Glc (3rarr1)
Rebaudioside C β-Glcβ-Glc -α-Rha(2rarr1)
I
β-Glc (3rarr 1)
Dulcoside A β-Glc β-Glc - α -Rha(2rarrl)
Rubusoside β-Glc β-Glc
Steviolbioside H β-Glc - β-Glc (2rarr1)
RebaudiosideB H β-Glc - β-Glc (2rarr1)
I
β-Glc (3rarr1)
Steviol (RI = R2 = H) is the aglycone of the steviol glycosides Glc and Rha
represent respectively glucose and rhamnose sugar moieties
The seven specific steviol glycosides
The component glycosides
of particular interest for
stevia leaf sweetening
property are stevioside and
Reb A
Powdered Stevia extracts
with 85ndash97 percent Sweet
glycosides (200-300 times
sweeter than sugar
(Sucrose)
Always Keep In
Mind We are
cultivating Stevia
for its leaves
bull A variety of soil types ranging from course
textured sands to well drained loamsare suitable
for growing stevia
but not heavy clay or poorly drained sites
bull Stevia requires very good drainage system
bull Stevia can be grown on a wide range of soil with
pH range from 6 up to 9
bull soil should not be saline
bull Soils with very heavy clay content or high pH
should also be treated or avoided
Before planting each and every
soil sample should be properly
tested and analyzed by an expert
Soil type
bull Climate bull The plant is climatically suited to climates from
temperate to sub-tropical where temperatures
range between 21-43degC with an average of
24degC
bull During the growing season it
seems to thrive in a temperature
range of 15 degC to 34degC provided all
input resources and good
management practices are
incorporated
bull Plant Varieties
bull There are many of Stevia rebaudiana
varieties developed all around the world
These varieties have been developed
for different climate requirements and
Stevioside and rebaudioside content
bull The minimum market requirement of
Stevioside and rebaudioside is 9
bull The grower should select proper
varieties with adequate guarantees from
the planting material suppliers about
minimum assured stevioside and
rebaudioside contents
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
Stevia was first brought to the attention of
the US government in 1918 for the US
Department of Agriculture
In France in 1931 that would later prove
significant There two chemists isolated
the most prevalent of several compounds
that give the stevia leaf its sweet taste a
pure white crystalline extract they named
stevioside
Today the global interests in stevia has
increased and the spread of the stevia
phenomenon will not be limited to a certain
countries
Stevia is cultivated and used in food
elsewhere in East Asia including in China
(since 1984) Korea Taiwan Thailand India
and Malaysia It can also be found in Saint
Kitts and Nevis in parts of South America
(Brazil Colombia Peru Paraguay and
Uruguay) and in Israel China is the worlds
largest exporter of Stevioside
Will sugar always be more advantageous than Kaa-he-e We cannot suppose this The superiority of sugar as an energetic food will not be contested but this does not stop our plant from being stronger as a sweetener
Moises N Bertoni
December 1905 Kaa-he-e Its Nature and Its Properties
Fresh leaves This is the simplest form of Stevia in
Japan they may be purchased loose or in tea bags They
are 15 to 30 times sweeter than sugar
Dried leaves They are 10 to 15 times sweeter than
sugar They have the same uses as fresh leaves and to
extract the Stevioside
Powdered or ground leaves They can be
found in bulk form and in tea bags used as a flavor
enhancer or sweetener in teas salads fruit and coffee
among others
Byproducts (plant remains) The remaining
parts of the plant including stems seeds flowers and even
leaves that were not classified for industrialization are
collected and processed into animal feed or fertilizers
as a mix with silage for animal food or to make a compost
to be use as a fertilizer
Fresh stevia leaves
Dry stevia leaves
Ground stevia leaves
Stevia Liquid Extracts These are
concentrated syrups made from the dried leaves
(dark liquid extracts) or from powdered Steviosides
(clear liquid extracts) in a base of water or alcohol
Used for the sweetening of beverages
Stevia Powders Powdered Stevia extracts
with 40ndash50 percent Sweet glycosides (more than
100 times sweeter than sugar) and Powdered
Stevia extracts with 85ndash97 percent Sweet
glycosides (200-300 times sweeter than sugar)
Stevia liquid extracts
Stevia extracted powders
Weight reduction
Antimicrobial and anti-plaque properties
Non-toxic
Calorie free
Tonic for pancreas
Hypertension
Increase energy levels
Benefits and uses of Stevia
Food usesMaximum use level
(mg Steviol glycosideskg food)
Desserts 500Cold confectionery 500Pickles 1000Sweetcorn 200Biscuits 300Breverages 500Yogurt 500Sauces 1000Delicacies 1000Bread 160
The global market for non-sugar sweeteners in 2011 was at about $92
billion and is expected to reach $93 billion in 2012 The market is expected
to rise at a compound annual growth rate (CAGR) of 11 and reach nearly
$99 billion by 2016 The North American market is estimated to reach
nearly $59 billion in 2012 and The European market represents the second
largest market category with the expected sale of nearly $19 billion in
2012
0
1000
2000
3000
4000
5000
6000
2009 2010 2011 2016
North america Europe Rest of the world
Millio
n U
SD
Sited from httpwwwprlogorg11726488-global-markets-for-non-sugar-sweetenershtml
Since the Stevia extract Rebaudioside A (Reb-A) received
GRAS approval by the US Food and Drug Administration (FDA)
on 17 December 2008 and the approval of the European
Commission on 2 December 2011 there have been several
hundred product launches by food and beverage companies
Steviarsquos status as a global ingredient was secured
with its incorporation into leading beverage
brands manufactured by Coca-Cola and Pepsi
companies in 2009
Now the worldrsquos largest natural
sweetness agent market opened the
door to the Stevia extract of Stevia
extract exports entering the
accelerated growth period
EUROPE9
SOUTH AMERICA
24
NORTH AMERICA
30
ASIA PACIFIC36
OTHERS1
Sited from
httpissuucomzenith_internationaldocsstevia_report_sample
Zenith forecasts that the global market for stevia will reach 11000 metric
tons by 2014 equivalent to US$825 million by value
0
05
1
15
2
25
3
35
2006 2007 2008 2009 2010
(X 1000 ton)
Sited from httpissuucomzenith_internationaldocsstevia_report_sample
One Feddan of stevia produce 4 to 6 tonsyear
(3 harvests) of Dry Leaves
The International price of dry leaves (ge 10
stevisoid) range from 2 to 4 kg USD
Stevia harvest 3 times a year under Egyptian
conditions
International price of 97 stevasoid range from
100 to 150 USDkg
Stevia stay in the field for several years (3 to 5)
years
YEAR 1 YEAR 2 YEAR 3 YEAR 4 TOTAL
Total costsyear (USD) 3061 2611 2611 2611 10896
AVERAGE PRODUCTION (TON)YEAR 45 45 45 45 18
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 9000 9000 9000 9000 36000
NET PROFIT 5938 6388 6388 6388 25103
OF PROFIT 1939 2446 2446 2446 2303
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 3 3 3 3 12
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 6000 6000 6000 6000 24000
NET PROFIT 2938333 3388333 3388333 3388333 1310333
OF PROFIT 959 1297 1297 1297 1202
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 2 2 2 2 8
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME (USD) 4000 4000 4000 4000 16000
NET PROFIT (USD) 9383333 1388333 1388333 1388333 5103333
OF PROFIT 306 531 531 531 468
From the current study we can notice that the major
costs are the field rent (5000 LE = 833$) and the
starter transplants (8000 LE = 1333$) From this
point to reduce the production costs it is suggested
to focus on producing stevia in the new reclaimed
lands which will lead to reduce the rent cost from
30 to 50 Also a system for producing stevia
seedlings (nurseries) to use as a starter transplants
should be introduced to the producers as a way to
reduce the starter material costs more than 50
One more advantage of cultivating stevia in new
reclaimed lands is the easy use of mechanization
and for setting up modified irrigation systems and
agriculture practices suitable for stevia
bull Stevia is an obligate short day plant with a critical day length of about 11-13 h
bull Long‐days delay flowering and increase stevasoid accumulation Extensive variability within populations for day length sensitivity has been reported
Stevia belongs to
Class Magnoliopsida (Dicotyledons)
Order Asterales
Family Asteraceae or Compositae
Stevia rebaudiana has many commonly
known Synonyms Sweet herb sweet
leaf sugar leaf and honey leaf or simply
Stevia
(مثل عباد الشمس) العائله المركبه
(و زيادة فترات النهار تؤخر االزهار و تسيد من ترجمع االستيفاسىيد( ساعه نهار 13-11)من نباتات النهار القصير
bull The RootThe root of stevia plant is fibrous filamentary
and perennial abundantly branched and
strain distributed near the surface and is
the only organ of the plant that does not
contain stevioside Stem
Root
Foliage
جذور نبات االستيفيا ليفيه فتيلية والمعمرة متشعبه و موزعه بالقرب من سطح
التربه وهي الجهاز الوحيد من النبات الذي ال يحتوي علي مادة االستيفاسويد
المحليه
bull The stemThe stem is annual sub-woody more or less
pubescent with a tendency to lean is more
or less branched During early development
does not have branches becoming
multicaule after the first growing season
coming to produce up to 20 stems in 3 to 4
years Under optimal conditions the stem
can reach five feet in height
Stem
Root
Foliage
bull The leafThe leaves are oval or elliptic lanceolate
small simple toothed edge or margin
sometimes in whorls somewhat hairy
The leaf is the organ with the highest
content of sweetener
Stem
Root
Foliage
ورقه بسيطة بيضاويه صغيره مسننه
bull The FlowersStevia classified as a short-day plant
placing the critical photoperiod at
12 to 13 hours depending on the
ecotype
Flowers are hermaphrodite
small whitish in terminal or axillary short
chapters grouped in corymbose panicles
االزهار صغيره خنثي بيضاء في مجاميع
The hermaphrodite flower is small whitish in
terminal or axillary short chapters grouped in
corymbose panicles A plant takes more than
a month to produce all their flowers The
plant is self-incompatible sporophytic type
and classified as obligatory
apomictic Pollination is by insects
Compound name R1 R2
Stevioside β-Glc β-Glc-β-Glc(2rarr1)
RebaudiosideA β-Glc β-Glc-β-Glc
I
β-Glc (3rarr1)
Rebaudioside C β-Glcβ-Glc -α-Rha(2rarr1)
I
β-Glc (3rarr 1)
Dulcoside A β-Glc β-Glc - α -Rha(2rarrl)
Rubusoside β-Glc β-Glc
Steviolbioside H β-Glc - β-Glc (2rarr1)
RebaudiosideB H β-Glc - β-Glc (2rarr1)
I
β-Glc (3rarr1)
Steviol (RI = R2 = H) is the aglycone of the steviol glycosides Glc and Rha
represent respectively glucose and rhamnose sugar moieties
The seven specific steviol glycosides
The component glycosides
of particular interest for
stevia leaf sweetening
property are stevioside and
Reb A
Powdered Stevia extracts
with 85ndash97 percent Sweet
glycosides (200-300 times
sweeter than sugar
(Sucrose)
Always Keep In
Mind We are
cultivating Stevia
for its leaves
bull A variety of soil types ranging from course
textured sands to well drained loamsare suitable
for growing stevia
but not heavy clay or poorly drained sites
bull Stevia requires very good drainage system
bull Stevia can be grown on a wide range of soil with
pH range from 6 up to 9
bull soil should not be saline
bull Soils with very heavy clay content or high pH
should also be treated or avoided
Before planting each and every
soil sample should be properly
tested and analyzed by an expert
Soil type
bull Climate bull The plant is climatically suited to climates from
temperate to sub-tropical where temperatures
range between 21-43degC with an average of
24degC
bull During the growing season it
seems to thrive in a temperature
range of 15 degC to 34degC provided all
input resources and good
management practices are
incorporated
bull Plant Varieties
bull There are many of Stevia rebaudiana
varieties developed all around the world
These varieties have been developed
for different climate requirements and
Stevioside and rebaudioside content
bull The minimum market requirement of
Stevioside and rebaudioside is 9
bull The grower should select proper
varieties with adequate guarantees from
the planting material suppliers about
minimum assured stevioside and
rebaudioside contents
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
Today the global interests in stevia has
increased and the spread of the stevia
phenomenon will not be limited to a certain
countries
Stevia is cultivated and used in food
elsewhere in East Asia including in China
(since 1984) Korea Taiwan Thailand India
and Malaysia It can also be found in Saint
Kitts and Nevis in parts of South America
(Brazil Colombia Peru Paraguay and
Uruguay) and in Israel China is the worlds
largest exporter of Stevioside
Will sugar always be more advantageous than Kaa-he-e We cannot suppose this The superiority of sugar as an energetic food will not be contested but this does not stop our plant from being stronger as a sweetener
Moises N Bertoni
December 1905 Kaa-he-e Its Nature and Its Properties
Fresh leaves This is the simplest form of Stevia in
Japan they may be purchased loose or in tea bags They
are 15 to 30 times sweeter than sugar
Dried leaves They are 10 to 15 times sweeter than
sugar They have the same uses as fresh leaves and to
extract the Stevioside
Powdered or ground leaves They can be
found in bulk form and in tea bags used as a flavor
enhancer or sweetener in teas salads fruit and coffee
among others
Byproducts (plant remains) The remaining
parts of the plant including stems seeds flowers and even
leaves that were not classified for industrialization are
collected and processed into animal feed or fertilizers
as a mix with silage for animal food or to make a compost
to be use as a fertilizer
Fresh stevia leaves
Dry stevia leaves
Ground stevia leaves
Stevia Liquid Extracts These are
concentrated syrups made from the dried leaves
(dark liquid extracts) or from powdered Steviosides
(clear liquid extracts) in a base of water or alcohol
Used for the sweetening of beverages
Stevia Powders Powdered Stevia extracts
with 40ndash50 percent Sweet glycosides (more than
100 times sweeter than sugar) and Powdered
Stevia extracts with 85ndash97 percent Sweet
glycosides (200-300 times sweeter than sugar)
Stevia liquid extracts
Stevia extracted powders
Weight reduction
Antimicrobial and anti-plaque properties
Non-toxic
Calorie free
Tonic for pancreas
Hypertension
Increase energy levels
Benefits and uses of Stevia
Food usesMaximum use level
(mg Steviol glycosideskg food)
Desserts 500Cold confectionery 500Pickles 1000Sweetcorn 200Biscuits 300Breverages 500Yogurt 500Sauces 1000Delicacies 1000Bread 160
The global market for non-sugar sweeteners in 2011 was at about $92
billion and is expected to reach $93 billion in 2012 The market is expected
to rise at a compound annual growth rate (CAGR) of 11 and reach nearly
$99 billion by 2016 The North American market is estimated to reach
nearly $59 billion in 2012 and The European market represents the second
largest market category with the expected sale of nearly $19 billion in
2012
0
1000
2000
3000
4000
5000
6000
2009 2010 2011 2016
North america Europe Rest of the world
Millio
n U
SD
Sited from httpwwwprlogorg11726488-global-markets-for-non-sugar-sweetenershtml
Since the Stevia extract Rebaudioside A (Reb-A) received
GRAS approval by the US Food and Drug Administration (FDA)
on 17 December 2008 and the approval of the European
Commission on 2 December 2011 there have been several
hundred product launches by food and beverage companies
Steviarsquos status as a global ingredient was secured
with its incorporation into leading beverage
brands manufactured by Coca-Cola and Pepsi
companies in 2009
Now the worldrsquos largest natural
sweetness agent market opened the
door to the Stevia extract of Stevia
extract exports entering the
accelerated growth period
EUROPE9
SOUTH AMERICA
24
NORTH AMERICA
30
ASIA PACIFIC36
OTHERS1
Sited from
httpissuucomzenith_internationaldocsstevia_report_sample
Zenith forecasts that the global market for stevia will reach 11000 metric
tons by 2014 equivalent to US$825 million by value
0
05
1
15
2
25
3
35
2006 2007 2008 2009 2010
(X 1000 ton)
Sited from httpissuucomzenith_internationaldocsstevia_report_sample
One Feddan of stevia produce 4 to 6 tonsyear
(3 harvests) of Dry Leaves
The International price of dry leaves (ge 10
stevisoid) range from 2 to 4 kg USD
Stevia harvest 3 times a year under Egyptian
conditions
International price of 97 stevasoid range from
100 to 150 USDkg
Stevia stay in the field for several years (3 to 5)
years
YEAR 1 YEAR 2 YEAR 3 YEAR 4 TOTAL
Total costsyear (USD) 3061 2611 2611 2611 10896
AVERAGE PRODUCTION (TON)YEAR 45 45 45 45 18
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 9000 9000 9000 9000 36000
NET PROFIT 5938 6388 6388 6388 25103
OF PROFIT 1939 2446 2446 2446 2303
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 3 3 3 3 12
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 6000 6000 6000 6000 24000
NET PROFIT 2938333 3388333 3388333 3388333 1310333
OF PROFIT 959 1297 1297 1297 1202
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 2 2 2 2 8
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME (USD) 4000 4000 4000 4000 16000
NET PROFIT (USD) 9383333 1388333 1388333 1388333 5103333
OF PROFIT 306 531 531 531 468
From the current study we can notice that the major
costs are the field rent (5000 LE = 833$) and the
starter transplants (8000 LE = 1333$) From this
point to reduce the production costs it is suggested
to focus on producing stevia in the new reclaimed
lands which will lead to reduce the rent cost from
30 to 50 Also a system for producing stevia
seedlings (nurseries) to use as a starter transplants
should be introduced to the producers as a way to
reduce the starter material costs more than 50
One more advantage of cultivating stevia in new
reclaimed lands is the easy use of mechanization
and for setting up modified irrigation systems and
agriculture practices suitable for stevia
bull Stevia is an obligate short day plant with a critical day length of about 11-13 h
bull Long‐days delay flowering and increase stevasoid accumulation Extensive variability within populations for day length sensitivity has been reported
Stevia belongs to
Class Magnoliopsida (Dicotyledons)
Order Asterales
Family Asteraceae or Compositae
Stevia rebaudiana has many commonly
known Synonyms Sweet herb sweet
leaf sugar leaf and honey leaf or simply
Stevia
(مثل عباد الشمس) العائله المركبه
(و زيادة فترات النهار تؤخر االزهار و تسيد من ترجمع االستيفاسىيد( ساعه نهار 13-11)من نباتات النهار القصير
bull The RootThe root of stevia plant is fibrous filamentary
and perennial abundantly branched and
strain distributed near the surface and is
the only organ of the plant that does not
contain stevioside Stem
Root
Foliage
جذور نبات االستيفيا ليفيه فتيلية والمعمرة متشعبه و موزعه بالقرب من سطح
التربه وهي الجهاز الوحيد من النبات الذي ال يحتوي علي مادة االستيفاسويد
المحليه
bull The stemThe stem is annual sub-woody more or less
pubescent with a tendency to lean is more
or less branched During early development
does not have branches becoming
multicaule after the first growing season
coming to produce up to 20 stems in 3 to 4
years Under optimal conditions the stem
can reach five feet in height
Stem
Root
Foliage
bull The leafThe leaves are oval or elliptic lanceolate
small simple toothed edge or margin
sometimes in whorls somewhat hairy
The leaf is the organ with the highest
content of sweetener
Stem
Root
Foliage
ورقه بسيطة بيضاويه صغيره مسننه
bull The FlowersStevia classified as a short-day plant
placing the critical photoperiod at
12 to 13 hours depending on the
ecotype
Flowers are hermaphrodite
small whitish in terminal or axillary short
chapters grouped in corymbose panicles
االزهار صغيره خنثي بيضاء في مجاميع
The hermaphrodite flower is small whitish in
terminal or axillary short chapters grouped in
corymbose panicles A plant takes more than
a month to produce all their flowers The
plant is self-incompatible sporophytic type
and classified as obligatory
apomictic Pollination is by insects
Compound name R1 R2
Stevioside β-Glc β-Glc-β-Glc(2rarr1)
RebaudiosideA β-Glc β-Glc-β-Glc
I
β-Glc (3rarr1)
Rebaudioside C β-Glcβ-Glc -α-Rha(2rarr1)
I
β-Glc (3rarr 1)
Dulcoside A β-Glc β-Glc - α -Rha(2rarrl)
Rubusoside β-Glc β-Glc
Steviolbioside H β-Glc - β-Glc (2rarr1)
RebaudiosideB H β-Glc - β-Glc (2rarr1)
I
β-Glc (3rarr1)
Steviol (RI = R2 = H) is the aglycone of the steviol glycosides Glc and Rha
represent respectively glucose and rhamnose sugar moieties
The seven specific steviol glycosides
The component glycosides
of particular interest for
stevia leaf sweetening
property are stevioside and
Reb A
Powdered Stevia extracts
with 85ndash97 percent Sweet
glycosides (200-300 times
sweeter than sugar
(Sucrose)
Always Keep In
Mind We are
cultivating Stevia
for its leaves
bull A variety of soil types ranging from course
textured sands to well drained loamsare suitable
for growing stevia
but not heavy clay or poorly drained sites
bull Stevia requires very good drainage system
bull Stevia can be grown on a wide range of soil with
pH range from 6 up to 9
bull soil should not be saline
bull Soils with very heavy clay content or high pH
should also be treated or avoided
Before planting each and every
soil sample should be properly
tested and analyzed by an expert
Soil type
bull Climate bull The plant is climatically suited to climates from
temperate to sub-tropical where temperatures
range between 21-43degC with an average of
24degC
bull During the growing season it
seems to thrive in a temperature
range of 15 degC to 34degC provided all
input resources and good
management practices are
incorporated
bull Plant Varieties
bull There are many of Stevia rebaudiana
varieties developed all around the world
These varieties have been developed
for different climate requirements and
Stevioside and rebaudioside content
bull The minimum market requirement of
Stevioside and rebaudioside is 9
bull The grower should select proper
varieties with adequate guarantees from
the planting material suppliers about
minimum assured stevioside and
rebaudioside contents
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
Will sugar always be more advantageous than Kaa-he-e We cannot suppose this The superiority of sugar as an energetic food will not be contested but this does not stop our plant from being stronger as a sweetener
Moises N Bertoni
December 1905 Kaa-he-e Its Nature and Its Properties
Fresh leaves This is the simplest form of Stevia in
Japan they may be purchased loose or in tea bags They
are 15 to 30 times sweeter than sugar
Dried leaves They are 10 to 15 times sweeter than
sugar They have the same uses as fresh leaves and to
extract the Stevioside
Powdered or ground leaves They can be
found in bulk form and in tea bags used as a flavor
enhancer or sweetener in teas salads fruit and coffee
among others
Byproducts (plant remains) The remaining
parts of the plant including stems seeds flowers and even
leaves that were not classified for industrialization are
collected and processed into animal feed or fertilizers
as a mix with silage for animal food or to make a compost
to be use as a fertilizer
Fresh stevia leaves
Dry stevia leaves
Ground stevia leaves
Stevia Liquid Extracts These are
concentrated syrups made from the dried leaves
(dark liquid extracts) or from powdered Steviosides
(clear liquid extracts) in a base of water or alcohol
Used for the sweetening of beverages
Stevia Powders Powdered Stevia extracts
with 40ndash50 percent Sweet glycosides (more than
100 times sweeter than sugar) and Powdered
Stevia extracts with 85ndash97 percent Sweet
glycosides (200-300 times sweeter than sugar)
Stevia liquid extracts
Stevia extracted powders
Weight reduction
Antimicrobial and anti-plaque properties
Non-toxic
Calorie free
Tonic for pancreas
Hypertension
Increase energy levels
Benefits and uses of Stevia
Food usesMaximum use level
(mg Steviol glycosideskg food)
Desserts 500Cold confectionery 500Pickles 1000Sweetcorn 200Biscuits 300Breverages 500Yogurt 500Sauces 1000Delicacies 1000Bread 160
The global market for non-sugar sweeteners in 2011 was at about $92
billion and is expected to reach $93 billion in 2012 The market is expected
to rise at a compound annual growth rate (CAGR) of 11 and reach nearly
$99 billion by 2016 The North American market is estimated to reach
nearly $59 billion in 2012 and The European market represents the second
largest market category with the expected sale of nearly $19 billion in
2012
0
1000
2000
3000
4000
5000
6000
2009 2010 2011 2016
North america Europe Rest of the world
Millio
n U
SD
Sited from httpwwwprlogorg11726488-global-markets-for-non-sugar-sweetenershtml
Since the Stevia extract Rebaudioside A (Reb-A) received
GRAS approval by the US Food and Drug Administration (FDA)
on 17 December 2008 and the approval of the European
Commission on 2 December 2011 there have been several
hundred product launches by food and beverage companies
Steviarsquos status as a global ingredient was secured
with its incorporation into leading beverage
brands manufactured by Coca-Cola and Pepsi
companies in 2009
Now the worldrsquos largest natural
sweetness agent market opened the
door to the Stevia extract of Stevia
extract exports entering the
accelerated growth period
EUROPE9
SOUTH AMERICA
24
NORTH AMERICA
30
ASIA PACIFIC36
OTHERS1
Sited from
httpissuucomzenith_internationaldocsstevia_report_sample
Zenith forecasts that the global market for stevia will reach 11000 metric
tons by 2014 equivalent to US$825 million by value
0
05
1
15
2
25
3
35
2006 2007 2008 2009 2010
(X 1000 ton)
Sited from httpissuucomzenith_internationaldocsstevia_report_sample
One Feddan of stevia produce 4 to 6 tonsyear
(3 harvests) of Dry Leaves
The International price of dry leaves (ge 10
stevisoid) range from 2 to 4 kg USD
Stevia harvest 3 times a year under Egyptian
conditions
International price of 97 stevasoid range from
100 to 150 USDkg
Stevia stay in the field for several years (3 to 5)
years
YEAR 1 YEAR 2 YEAR 3 YEAR 4 TOTAL
Total costsyear (USD) 3061 2611 2611 2611 10896
AVERAGE PRODUCTION (TON)YEAR 45 45 45 45 18
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 9000 9000 9000 9000 36000
NET PROFIT 5938 6388 6388 6388 25103
OF PROFIT 1939 2446 2446 2446 2303
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 3 3 3 3 12
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 6000 6000 6000 6000 24000
NET PROFIT 2938333 3388333 3388333 3388333 1310333
OF PROFIT 959 1297 1297 1297 1202
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 2 2 2 2 8
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME (USD) 4000 4000 4000 4000 16000
NET PROFIT (USD) 9383333 1388333 1388333 1388333 5103333
OF PROFIT 306 531 531 531 468
From the current study we can notice that the major
costs are the field rent (5000 LE = 833$) and the
starter transplants (8000 LE = 1333$) From this
point to reduce the production costs it is suggested
to focus on producing stevia in the new reclaimed
lands which will lead to reduce the rent cost from
30 to 50 Also a system for producing stevia
seedlings (nurseries) to use as a starter transplants
should be introduced to the producers as a way to
reduce the starter material costs more than 50
One more advantage of cultivating stevia in new
reclaimed lands is the easy use of mechanization
and for setting up modified irrigation systems and
agriculture practices suitable for stevia
bull Stevia is an obligate short day plant with a critical day length of about 11-13 h
bull Long‐days delay flowering and increase stevasoid accumulation Extensive variability within populations for day length sensitivity has been reported
Stevia belongs to
Class Magnoliopsida (Dicotyledons)
Order Asterales
Family Asteraceae or Compositae
Stevia rebaudiana has many commonly
known Synonyms Sweet herb sweet
leaf sugar leaf and honey leaf or simply
Stevia
(مثل عباد الشمس) العائله المركبه
(و زيادة فترات النهار تؤخر االزهار و تسيد من ترجمع االستيفاسىيد( ساعه نهار 13-11)من نباتات النهار القصير
bull The RootThe root of stevia plant is fibrous filamentary
and perennial abundantly branched and
strain distributed near the surface and is
the only organ of the plant that does not
contain stevioside Stem
Root
Foliage
جذور نبات االستيفيا ليفيه فتيلية والمعمرة متشعبه و موزعه بالقرب من سطح
التربه وهي الجهاز الوحيد من النبات الذي ال يحتوي علي مادة االستيفاسويد
المحليه
bull The stemThe stem is annual sub-woody more or less
pubescent with a tendency to lean is more
or less branched During early development
does not have branches becoming
multicaule after the first growing season
coming to produce up to 20 stems in 3 to 4
years Under optimal conditions the stem
can reach five feet in height
Stem
Root
Foliage
bull The leafThe leaves are oval or elliptic lanceolate
small simple toothed edge or margin
sometimes in whorls somewhat hairy
The leaf is the organ with the highest
content of sweetener
Stem
Root
Foliage
ورقه بسيطة بيضاويه صغيره مسننه
bull The FlowersStevia classified as a short-day plant
placing the critical photoperiod at
12 to 13 hours depending on the
ecotype
Flowers are hermaphrodite
small whitish in terminal or axillary short
chapters grouped in corymbose panicles
االزهار صغيره خنثي بيضاء في مجاميع
The hermaphrodite flower is small whitish in
terminal or axillary short chapters grouped in
corymbose panicles A plant takes more than
a month to produce all their flowers The
plant is self-incompatible sporophytic type
and classified as obligatory
apomictic Pollination is by insects
Compound name R1 R2
Stevioside β-Glc β-Glc-β-Glc(2rarr1)
RebaudiosideA β-Glc β-Glc-β-Glc
I
β-Glc (3rarr1)
Rebaudioside C β-Glcβ-Glc -α-Rha(2rarr1)
I
β-Glc (3rarr 1)
Dulcoside A β-Glc β-Glc - α -Rha(2rarrl)
Rubusoside β-Glc β-Glc
Steviolbioside H β-Glc - β-Glc (2rarr1)
RebaudiosideB H β-Glc - β-Glc (2rarr1)
I
β-Glc (3rarr1)
Steviol (RI = R2 = H) is the aglycone of the steviol glycosides Glc and Rha
represent respectively glucose and rhamnose sugar moieties
The seven specific steviol glycosides
The component glycosides
of particular interest for
stevia leaf sweetening
property are stevioside and
Reb A
Powdered Stevia extracts
with 85ndash97 percent Sweet
glycosides (200-300 times
sweeter than sugar
(Sucrose)
Always Keep In
Mind We are
cultivating Stevia
for its leaves
bull A variety of soil types ranging from course
textured sands to well drained loamsare suitable
for growing stevia
but not heavy clay or poorly drained sites
bull Stevia requires very good drainage system
bull Stevia can be grown on a wide range of soil with
pH range from 6 up to 9
bull soil should not be saline
bull Soils with very heavy clay content or high pH
should also be treated or avoided
Before planting each and every
soil sample should be properly
tested and analyzed by an expert
Soil type
bull Climate bull The plant is climatically suited to climates from
temperate to sub-tropical where temperatures
range between 21-43degC with an average of
24degC
bull During the growing season it
seems to thrive in a temperature
range of 15 degC to 34degC provided all
input resources and good
management practices are
incorporated
bull Plant Varieties
bull There are many of Stevia rebaudiana
varieties developed all around the world
These varieties have been developed
for different climate requirements and
Stevioside and rebaudioside content
bull The minimum market requirement of
Stevioside and rebaudioside is 9
bull The grower should select proper
varieties with adequate guarantees from
the planting material suppliers about
minimum assured stevioside and
rebaudioside contents
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
Fresh leaves This is the simplest form of Stevia in
Japan they may be purchased loose or in tea bags They
are 15 to 30 times sweeter than sugar
Dried leaves They are 10 to 15 times sweeter than
sugar They have the same uses as fresh leaves and to
extract the Stevioside
Powdered or ground leaves They can be
found in bulk form and in tea bags used as a flavor
enhancer or sweetener in teas salads fruit and coffee
among others
Byproducts (plant remains) The remaining
parts of the plant including stems seeds flowers and even
leaves that were not classified for industrialization are
collected and processed into animal feed or fertilizers
as a mix with silage for animal food or to make a compost
to be use as a fertilizer
Fresh stevia leaves
Dry stevia leaves
Ground stevia leaves
Stevia Liquid Extracts These are
concentrated syrups made from the dried leaves
(dark liquid extracts) or from powdered Steviosides
(clear liquid extracts) in a base of water or alcohol
Used for the sweetening of beverages
Stevia Powders Powdered Stevia extracts
with 40ndash50 percent Sweet glycosides (more than
100 times sweeter than sugar) and Powdered
Stevia extracts with 85ndash97 percent Sweet
glycosides (200-300 times sweeter than sugar)
Stevia liquid extracts
Stevia extracted powders
Weight reduction
Antimicrobial and anti-plaque properties
Non-toxic
Calorie free
Tonic for pancreas
Hypertension
Increase energy levels
Benefits and uses of Stevia
Food usesMaximum use level
(mg Steviol glycosideskg food)
Desserts 500Cold confectionery 500Pickles 1000Sweetcorn 200Biscuits 300Breverages 500Yogurt 500Sauces 1000Delicacies 1000Bread 160
The global market for non-sugar sweeteners in 2011 was at about $92
billion and is expected to reach $93 billion in 2012 The market is expected
to rise at a compound annual growth rate (CAGR) of 11 and reach nearly
$99 billion by 2016 The North American market is estimated to reach
nearly $59 billion in 2012 and The European market represents the second
largest market category with the expected sale of nearly $19 billion in
2012
0
1000
2000
3000
4000
5000
6000
2009 2010 2011 2016
North america Europe Rest of the world
Millio
n U
SD
Sited from httpwwwprlogorg11726488-global-markets-for-non-sugar-sweetenershtml
Since the Stevia extract Rebaudioside A (Reb-A) received
GRAS approval by the US Food and Drug Administration (FDA)
on 17 December 2008 and the approval of the European
Commission on 2 December 2011 there have been several
hundred product launches by food and beverage companies
Steviarsquos status as a global ingredient was secured
with its incorporation into leading beverage
brands manufactured by Coca-Cola and Pepsi
companies in 2009
Now the worldrsquos largest natural
sweetness agent market opened the
door to the Stevia extract of Stevia
extract exports entering the
accelerated growth period
EUROPE9
SOUTH AMERICA
24
NORTH AMERICA
30
ASIA PACIFIC36
OTHERS1
Sited from
httpissuucomzenith_internationaldocsstevia_report_sample
Zenith forecasts that the global market for stevia will reach 11000 metric
tons by 2014 equivalent to US$825 million by value
0
05
1
15
2
25
3
35
2006 2007 2008 2009 2010
(X 1000 ton)
Sited from httpissuucomzenith_internationaldocsstevia_report_sample
One Feddan of stevia produce 4 to 6 tonsyear
(3 harvests) of Dry Leaves
The International price of dry leaves (ge 10
stevisoid) range from 2 to 4 kg USD
Stevia harvest 3 times a year under Egyptian
conditions
International price of 97 stevasoid range from
100 to 150 USDkg
Stevia stay in the field for several years (3 to 5)
years
YEAR 1 YEAR 2 YEAR 3 YEAR 4 TOTAL
Total costsyear (USD) 3061 2611 2611 2611 10896
AVERAGE PRODUCTION (TON)YEAR 45 45 45 45 18
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 9000 9000 9000 9000 36000
NET PROFIT 5938 6388 6388 6388 25103
OF PROFIT 1939 2446 2446 2446 2303
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 3 3 3 3 12
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 6000 6000 6000 6000 24000
NET PROFIT 2938333 3388333 3388333 3388333 1310333
OF PROFIT 959 1297 1297 1297 1202
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 2 2 2 2 8
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME (USD) 4000 4000 4000 4000 16000
NET PROFIT (USD) 9383333 1388333 1388333 1388333 5103333
OF PROFIT 306 531 531 531 468
From the current study we can notice that the major
costs are the field rent (5000 LE = 833$) and the
starter transplants (8000 LE = 1333$) From this
point to reduce the production costs it is suggested
to focus on producing stevia in the new reclaimed
lands which will lead to reduce the rent cost from
30 to 50 Also a system for producing stevia
seedlings (nurseries) to use as a starter transplants
should be introduced to the producers as a way to
reduce the starter material costs more than 50
One more advantage of cultivating stevia in new
reclaimed lands is the easy use of mechanization
and for setting up modified irrigation systems and
agriculture practices suitable for stevia
bull Stevia is an obligate short day plant with a critical day length of about 11-13 h
bull Long‐days delay flowering and increase stevasoid accumulation Extensive variability within populations for day length sensitivity has been reported
Stevia belongs to
Class Magnoliopsida (Dicotyledons)
Order Asterales
Family Asteraceae or Compositae
Stevia rebaudiana has many commonly
known Synonyms Sweet herb sweet
leaf sugar leaf and honey leaf or simply
Stevia
(مثل عباد الشمس) العائله المركبه
(و زيادة فترات النهار تؤخر االزهار و تسيد من ترجمع االستيفاسىيد( ساعه نهار 13-11)من نباتات النهار القصير
bull The RootThe root of stevia plant is fibrous filamentary
and perennial abundantly branched and
strain distributed near the surface and is
the only organ of the plant that does not
contain stevioside Stem
Root
Foliage
جذور نبات االستيفيا ليفيه فتيلية والمعمرة متشعبه و موزعه بالقرب من سطح
التربه وهي الجهاز الوحيد من النبات الذي ال يحتوي علي مادة االستيفاسويد
المحليه
bull The stemThe stem is annual sub-woody more or less
pubescent with a tendency to lean is more
or less branched During early development
does not have branches becoming
multicaule after the first growing season
coming to produce up to 20 stems in 3 to 4
years Under optimal conditions the stem
can reach five feet in height
Stem
Root
Foliage
bull The leafThe leaves are oval or elliptic lanceolate
small simple toothed edge or margin
sometimes in whorls somewhat hairy
The leaf is the organ with the highest
content of sweetener
Stem
Root
Foliage
ورقه بسيطة بيضاويه صغيره مسننه
bull The FlowersStevia classified as a short-day plant
placing the critical photoperiod at
12 to 13 hours depending on the
ecotype
Flowers are hermaphrodite
small whitish in terminal or axillary short
chapters grouped in corymbose panicles
االزهار صغيره خنثي بيضاء في مجاميع
The hermaphrodite flower is small whitish in
terminal or axillary short chapters grouped in
corymbose panicles A plant takes more than
a month to produce all their flowers The
plant is self-incompatible sporophytic type
and classified as obligatory
apomictic Pollination is by insects
Compound name R1 R2
Stevioside β-Glc β-Glc-β-Glc(2rarr1)
RebaudiosideA β-Glc β-Glc-β-Glc
I
β-Glc (3rarr1)
Rebaudioside C β-Glcβ-Glc -α-Rha(2rarr1)
I
β-Glc (3rarr 1)
Dulcoside A β-Glc β-Glc - α -Rha(2rarrl)
Rubusoside β-Glc β-Glc
Steviolbioside H β-Glc - β-Glc (2rarr1)
RebaudiosideB H β-Glc - β-Glc (2rarr1)
I
β-Glc (3rarr1)
Steviol (RI = R2 = H) is the aglycone of the steviol glycosides Glc and Rha
represent respectively glucose and rhamnose sugar moieties
The seven specific steviol glycosides
The component glycosides
of particular interest for
stevia leaf sweetening
property are stevioside and
Reb A
Powdered Stevia extracts
with 85ndash97 percent Sweet
glycosides (200-300 times
sweeter than sugar
(Sucrose)
Always Keep In
Mind We are
cultivating Stevia
for its leaves
bull A variety of soil types ranging from course
textured sands to well drained loamsare suitable
for growing stevia
but not heavy clay or poorly drained sites
bull Stevia requires very good drainage system
bull Stevia can be grown on a wide range of soil with
pH range from 6 up to 9
bull soil should not be saline
bull Soils with very heavy clay content or high pH
should also be treated or avoided
Before planting each and every
soil sample should be properly
tested and analyzed by an expert
Soil type
bull Climate bull The plant is climatically suited to climates from
temperate to sub-tropical where temperatures
range between 21-43degC with an average of
24degC
bull During the growing season it
seems to thrive in a temperature
range of 15 degC to 34degC provided all
input resources and good
management practices are
incorporated
bull Plant Varieties
bull There are many of Stevia rebaudiana
varieties developed all around the world
These varieties have been developed
for different climate requirements and
Stevioside and rebaudioside content
bull The minimum market requirement of
Stevioside and rebaudioside is 9
bull The grower should select proper
varieties with adequate guarantees from
the planting material suppliers about
minimum assured stevioside and
rebaudioside contents
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
Stevia Liquid Extracts These are
concentrated syrups made from the dried leaves
(dark liquid extracts) or from powdered Steviosides
(clear liquid extracts) in a base of water or alcohol
Used for the sweetening of beverages
Stevia Powders Powdered Stevia extracts
with 40ndash50 percent Sweet glycosides (more than
100 times sweeter than sugar) and Powdered
Stevia extracts with 85ndash97 percent Sweet
glycosides (200-300 times sweeter than sugar)
Stevia liquid extracts
Stevia extracted powders
Weight reduction
Antimicrobial and anti-plaque properties
Non-toxic
Calorie free
Tonic for pancreas
Hypertension
Increase energy levels
Benefits and uses of Stevia
Food usesMaximum use level
(mg Steviol glycosideskg food)
Desserts 500Cold confectionery 500Pickles 1000Sweetcorn 200Biscuits 300Breverages 500Yogurt 500Sauces 1000Delicacies 1000Bread 160
The global market for non-sugar sweeteners in 2011 was at about $92
billion and is expected to reach $93 billion in 2012 The market is expected
to rise at a compound annual growth rate (CAGR) of 11 and reach nearly
$99 billion by 2016 The North American market is estimated to reach
nearly $59 billion in 2012 and The European market represents the second
largest market category with the expected sale of nearly $19 billion in
2012
0
1000
2000
3000
4000
5000
6000
2009 2010 2011 2016
North america Europe Rest of the world
Millio
n U
SD
Sited from httpwwwprlogorg11726488-global-markets-for-non-sugar-sweetenershtml
Since the Stevia extract Rebaudioside A (Reb-A) received
GRAS approval by the US Food and Drug Administration (FDA)
on 17 December 2008 and the approval of the European
Commission on 2 December 2011 there have been several
hundred product launches by food and beverage companies
Steviarsquos status as a global ingredient was secured
with its incorporation into leading beverage
brands manufactured by Coca-Cola and Pepsi
companies in 2009
Now the worldrsquos largest natural
sweetness agent market opened the
door to the Stevia extract of Stevia
extract exports entering the
accelerated growth period
EUROPE9
SOUTH AMERICA
24
NORTH AMERICA
30
ASIA PACIFIC36
OTHERS1
Sited from
httpissuucomzenith_internationaldocsstevia_report_sample
Zenith forecasts that the global market for stevia will reach 11000 metric
tons by 2014 equivalent to US$825 million by value
0
05
1
15
2
25
3
35
2006 2007 2008 2009 2010
(X 1000 ton)
Sited from httpissuucomzenith_internationaldocsstevia_report_sample
One Feddan of stevia produce 4 to 6 tonsyear
(3 harvests) of Dry Leaves
The International price of dry leaves (ge 10
stevisoid) range from 2 to 4 kg USD
Stevia harvest 3 times a year under Egyptian
conditions
International price of 97 stevasoid range from
100 to 150 USDkg
Stevia stay in the field for several years (3 to 5)
years
YEAR 1 YEAR 2 YEAR 3 YEAR 4 TOTAL
Total costsyear (USD) 3061 2611 2611 2611 10896
AVERAGE PRODUCTION (TON)YEAR 45 45 45 45 18
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 9000 9000 9000 9000 36000
NET PROFIT 5938 6388 6388 6388 25103
OF PROFIT 1939 2446 2446 2446 2303
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 3 3 3 3 12
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 6000 6000 6000 6000 24000
NET PROFIT 2938333 3388333 3388333 3388333 1310333
OF PROFIT 959 1297 1297 1297 1202
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 2 2 2 2 8
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME (USD) 4000 4000 4000 4000 16000
NET PROFIT (USD) 9383333 1388333 1388333 1388333 5103333
OF PROFIT 306 531 531 531 468
From the current study we can notice that the major
costs are the field rent (5000 LE = 833$) and the
starter transplants (8000 LE = 1333$) From this
point to reduce the production costs it is suggested
to focus on producing stevia in the new reclaimed
lands which will lead to reduce the rent cost from
30 to 50 Also a system for producing stevia
seedlings (nurseries) to use as a starter transplants
should be introduced to the producers as a way to
reduce the starter material costs more than 50
One more advantage of cultivating stevia in new
reclaimed lands is the easy use of mechanization
and for setting up modified irrigation systems and
agriculture practices suitable for stevia
bull Stevia is an obligate short day plant with a critical day length of about 11-13 h
bull Long‐days delay flowering and increase stevasoid accumulation Extensive variability within populations for day length sensitivity has been reported
Stevia belongs to
Class Magnoliopsida (Dicotyledons)
Order Asterales
Family Asteraceae or Compositae
Stevia rebaudiana has many commonly
known Synonyms Sweet herb sweet
leaf sugar leaf and honey leaf or simply
Stevia
(مثل عباد الشمس) العائله المركبه
(و زيادة فترات النهار تؤخر االزهار و تسيد من ترجمع االستيفاسىيد( ساعه نهار 13-11)من نباتات النهار القصير
bull The RootThe root of stevia plant is fibrous filamentary
and perennial abundantly branched and
strain distributed near the surface and is
the only organ of the plant that does not
contain stevioside Stem
Root
Foliage
جذور نبات االستيفيا ليفيه فتيلية والمعمرة متشعبه و موزعه بالقرب من سطح
التربه وهي الجهاز الوحيد من النبات الذي ال يحتوي علي مادة االستيفاسويد
المحليه
bull The stemThe stem is annual sub-woody more or less
pubescent with a tendency to lean is more
or less branched During early development
does not have branches becoming
multicaule after the first growing season
coming to produce up to 20 stems in 3 to 4
years Under optimal conditions the stem
can reach five feet in height
Stem
Root
Foliage
bull The leafThe leaves are oval or elliptic lanceolate
small simple toothed edge or margin
sometimes in whorls somewhat hairy
The leaf is the organ with the highest
content of sweetener
Stem
Root
Foliage
ورقه بسيطة بيضاويه صغيره مسننه
bull The FlowersStevia classified as a short-day plant
placing the critical photoperiod at
12 to 13 hours depending on the
ecotype
Flowers are hermaphrodite
small whitish in terminal or axillary short
chapters grouped in corymbose panicles
االزهار صغيره خنثي بيضاء في مجاميع
The hermaphrodite flower is small whitish in
terminal or axillary short chapters grouped in
corymbose panicles A plant takes more than
a month to produce all their flowers The
plant is self-incompatible sporophytic type
and classified as obligatory
apomictic Pollination is by insects
Compound name R1 R2
Stevioside β-Glc β-Glc-β-Glc(2rarr1)
RebaudiosideA β-Glc β-Glc-β-Glc
I
β-Glc (3rarr1)
Rebaudioside C β-Glcβ-Glc -α-Rha(2rarr1)
I
β-Glc (3rarr 1)
Dulcoside A β-Glc β-Glc - α -Rha(2rarrl)
Rubusoside β-Glc β-Glc
Steviolbioside H β-Glc - β-Glc (2rarr1)
RebaudiosideB H β-Glc - β-Glc (2rarr1)
I
β-Glc (3rarr1)
Steviol (RI = R2 = H) is the aglycone of the steviol glycosides Glc and Rha
represent respectively glucose and rhamnose sugar moieties
The seven specific steviol glycosides
The component glycosides
of particular interest for
stevia leaf sweetening
property are stevioside and
Reb A
Powdered Stevia extracts
with 85ndash97 percent Sweet
glycosides (200-300 times
sweeter than sugar
(Sucrose)
Always Keep In
Mind We are
cultivating Stevia
for its leaves
bull A variety of soil types ranging from course
textured sands to well drained loamsare suitable
for growing stevia
but not heavy clay or poorly drained sites
bull Stevia requires very good drainage system
bull Stevia can be grown on a wide range of soil with
pH range from 6 up to 9
bull soil should not be saline
bull Soils with very heavy clay content or high pH
should also be treated or avoided
Before planting each and every
soil sample should be properly
tested and analyzed by an expert
Soil type
bull Climate bull The plant is climatically suited to climates from
temperate to sub-tropical where temperatures
range between 21-43degC with an average of
24degC
bull During the growing season it
seems to thrive in a temperature
range of 15 degC to 34degC provided all
input resources and good
management practices are
incorporated
bull Plant Varieties
bull There are many of Stevia rebaudiana
varieties developed all around the world
These varieties have been developed
for different climate requirements and
Stevioside and rebaudioside content
bull The minimum market requirement of
Stevioside and rebaudioside is 9
bull The grower should select proper
varieties with adequate guarantees from
the planting material suppliers about
minimum assured stevioside and
rebaudioside contents
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
Weight reduction
Antimicrobial and anti-plaque properties
Non-toxic
Calorie free
Tonic for pancreas
Hypertension
Increase energy levels
Benefits and uses of Stevia
Food usesMaximum use level
(mg Steviol glycosideskg food)
Desserts 500Cold confectionery 500Pickles 1000Sweetcorn 200Biscuits 300Breverages 500Yogurt 500Sauces 1000Delicacies 1000Bread 160
The global market for non-sugar sweeteners in 2011 was at about $92
billion and is expected to reach $93 billion in 2012 The market is expected
to rise at a compound annual growth rate (CAGR) of 11 and reach nearly
$99 billion by 2016 The North American market is estimated to reach
nearly $59 billion in 2012 and The European market represents the second
largest market category with the expected sale of nearly $19 billion in
2012
0
1000
2000
3000
4000
5000
6000
2009 2010 2011 2016
North america Europe Rest of the world
Millio
n U
SD
Sited from httpwwwprlogorg11726488-global-markets-for-non-sugar-sweetenershtml
Since the Stevia extract Rebaudioside A (Reb-A) received
GRAS approval by the US Food and Drug Administration (FDA)
on 17 December 2008 and the approval of the European
Commission on 2 December 2011 there have been several
hundred product launches by food and beverage companies
Steviarsquos status as a global ingredient was secured
with its incorporation into leading beverage
brands manufactured by Coca-Cola and Pepsi
companies in 2009
Now the worldrsquos largest natural
sweetness agent market opened the
door to the Stevia extract of Stevia
extract exports entering the
accelerated growth period
EUROPE9
SOUTH AMERICA
24
NORTH AMERICA
30
ASIA PACIFIC36
OTHERS1
Sited from
httpissuucomzenith_internationaldocsstevia_report_sample
Zenith forecasts that the global market for stevia will reach 11000 metric
tons by 2014 equivalent to US$825 million by value
0
05
1
15
2
25
3
35
2006 2007 2008 2009 2010
(X 1000 ton)
Sited from httpissuucomzenith_internationaldocsstevia_report_sample
One Feddan of stevia produce 4 to 6 tonsyear
(3 harvests) of Dry Leaves
The International price of dry leaves (ge 10
stevisoid) range from 2 to 4 kg USD
Stevia harvest 3 times a year under Egyptian
conditions
International price of 97 stevasoid range from
100 to 150 USDkg
Stevia stay in the field for several years (3 to 5)
years
YEAR 1 YEAR 2 YEAR 3 YEAR 4 TOTAL
Total costsyear (USD) 3061 2611 2611 2611 10896
AVERAGE PRODUCTION (TON)YEAR 45 45 45 45 18
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 9000 9000 9000 9000 36000
NET PROFIT 5938 6388 6388 6388 25103
OF PROFIT 1939 2446 2446 2446 2303
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 3 3 3 3 12
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 6000 6000 6000 6000 24000
NET PROFIT 2938333 3388333 3388333 3388333 1310333
OF PROFIT 959 1297 1297 1297 1202
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 2 2 2 2 8
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME (USD) 4000 4000 4000 4000 16000
NET PROFIT (USD) 9383333 1388333 1388333 1388333 5103333
OF PROFIT 306 531 531 531 468
From the current study we can notice that the major
costs are the field rent (5000 LE = 833$) and the
starter transplants (8000 LE = 1333$) From this
point to reduce the production costs it is suggested
to focus on producing stevia in the new reclaimed
lands which will lead to reduce the rent cost from
30 to 50 Also a system for producing stevia
seedlings (nurseries) to use as a starter transplants
should be introduced to the producers as a way to
reduce the starter material costs more than 50
One more advantage of cultivating stevia in new
reclaimed lands is the easy use of mechanization
and for setting up modified irrigation systems and
agriculture practices suitable for stevia
bull Stevia is an obligate short day plant with a critical day length of about 11-13 h
bull Long‐days delay flowering and increase stevasoid accumulation Extensive variability within populations for day length sensitivity has been reported
Stevia belongs to
Class Magnoliopsida (Dicotyledons)
Order Asterales
Family Asteraceae or Compositae
Stevia rebaudiana has many commonly
known Synonyms Sweet herb sweet
leaf sugar leaf and honey leaf or simply
Stevia
(مثل عباد الشمس) العائله المركبه
(و زيادة فترات النهار تؤخر االزهار و تسيد من ترجمع االستيفاسىيد( ساعه نهار 13-11)من نباتات النهار القصير
bull The RootThe root of stevia plant is fibrous filamentary
and perennial abundantly branched and
strain distributed near the surface and is
the only organ of the plant that does not
contain stevioside Stem
Root
Foliage
جذور نبات االستيفيا ليفيه فتيلية والمعمرة متشعبه و موزعه بالقرب من سطح
التربه وهي الجهاز الوحيد من النبات الذي ال يحتوي علي مادة االستيفاسويد
المحليه
bull The stemThe stem is annual sub-woody more or less
pubescent with a tendency to lean is more
or less branched During early development
does not have branches becoming
multicaule after the first growing season
coming to produce up to 20 stems in 3 to 4
years Under optimal conditions the stem
can reach five feet in height
Stem
Root
Foliage
bull The leafThe leaves are oval or elliptic lanceolate
small simple toothed edge or margin
sometimes in whorls somewhat hairy
The leaf is the organ with the highest
content of sweetener
Stem
Root
Foliage
ورقه بسيطة بيضاويه صغيره مسننه
bull The FlowersStevia classified as a short-day plant
placing the critical photoperiod at
12 to 13 hours depending on the
ecotype
Flowers are hermaphrodite
small whitish in terminal or axillary short
chapters grouped in corymbose panicles
االزهار صغيره خنثي بيضاء في مجاميع
The hermaphrodite flower is small whitish in
terminal or axillary short chapters grouped in
corymbose panicles A plant takes more than
a month to produce all their flowers The
plant is self-incompatible sporophytic type
and classified as obligatory
apomictic Pollination is by insects
Compound name R1 R2
Stevioside β-Glc β-Glc-β-Glc(2rarr1)
RebaudiosideA β-Glc β-Glc-β-Glc
I
β-Glc (3rarr1)
Rebaudioside C β-Glcβ-Glc -α-Rha(2rarr1)
I
β-Glc (3rarr 1)
Dulcoside A β-Glc β-Glc - α -Rha(2rarrl)
Rubusoside β-Glc β-Glc
Steviolbioside H β-Glc - β-Glc (2rarr1)
RebaudiosideB H β-Glc - β-Glc (2rarr1)
I
β-Glc (3rarr1)
Steviol (RI = R2 = H) is the aglycone of the steviol glycosides Glc and Rha
represent respectively glucose and rhamnose sugar moieties
The seven specific steviol glycosides
The component glycosides
of particular interest for
stevia leaf sweetening
property are stevioside and
Reb A
Powdered Stevia extracts
with 85ndash97 percent Sweet
glycosides (200-300 times
sweeter than sugar
(Sucrose)
Always Keep In
Mind We are
cultivating Stevia
for its leaves
bull A variety of soil types ranging from course
textured sands to well drained loamsare suitable
for growing stevia
but not heavy clay or poorly drained sites
bull Stevia requires very good drainage system
bull Stevia can be grown on a wide range of soil with
pH range from 6 up to 9
bull soil should not be saline
bull Soils with very heavy clay content or high pH
should also be treated or avoided
Before planting each and every
soil sample should be properly
tested and analyzed by an expert
Soil type
bull Climate bull The plant is climatically suited to climates from
temperate to sub-tropical where temperatures
range between 21-43degC with an average of
24degC
bull During the growing season it
seems to thrive in a temperature
range of 15 degC to 34degC provided all
input resources and good
management practices are
incorporated
bull Plant Varieties
bull There are many of Stevia rebaudiana
varieties developed all around the world
These varieties have been developed
for different climate requirements and
Stevioside and rebaudioside content
bull The minimum market requirement of
Stevioside and rebaudioside is 9
bull The grower should select proper
varieties with adequate guarantees from
the planting material suppliers about
minimum assured stevioside and
rebaudioside contents
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
Food usesMaximum use level
(mg Steviol glycosideskg food)
Desserts 500Cold confectionery 500Pickles 1000Sweetcorn 200Biscuits 300Breverages 500Yogurt 500Sauces 1000Delicacies 1000Bread 160
The global market for non-sugar sweeteners in 2011 was at about $92
billion and is expected to reach $93 billion in 2012 The market is expected
to rise at a compound annual growth rate (CAGR) of 11 and reach nearly
$99 billion by 2016 The North American market is estimated to reach
nearly $59 billion in 2012 and The European market represents the second
largest market category with the expected sale of nearly $19 billion in
2012
0
1000
2000
3000
4000
5000
6000
2009 2010 2011 2016
North america Europe Rest of the world
Millio
n U
SD
Sited from httpwwwprlogorg11726488-global-markets-for-non-sugar-sweetenershtml
Since the Stevia extract Rebaudioside A (Reb-A) received
GRAS approval by the US Food and Drug Administration (FDA)
on 17 December 2008 and the approval of the European
Commission on 2 December 2011 there have been several
hundred product launches by food and beverage companies
Steviarsquos status as a global ingredient was secured
with its incorporation into leading beverage
brands manufactured by Coca-Cola and Pepsi
companies in 2009
Now the worldrsquos largest natural
sweetness agent market opened the
door to the Stevia extract of Stevia
extract exports entering the
accelerated growth period
EUROPE9
SOUTH AMERICA
24
NORTH AMERICA
30
ASIA PACIFIC36
OTHERS1
Sited from
httpissuucomzenith_internationaldocsstevia_report_sample
Zenith forecasts that the global market for stevia will reach 11000 metric
tons by 2014 equivalent to US$825 million by value
0
05
1
15
2
25
3
35
2006 2007 2008 2009 2010
(X 1000 ton)
Sited from httpissuucomzenith_internationaldocsstevia_report_sample
One Feddan of stevia produce 4 to 6 tonsyear
(3 harvests) of Dry Leaves
The International price of dry leaves (ge 10
stevisoid) range from 2 to 4 kg USD
Stevia harvest 3 times a year under Egyptian
conditions
International price of 97 stevasoid range from
100 to 150 USDkg
Stevia stay in the field for several years (3 to 5)
years
YEAR 1 YEAR 2 YEAR 3 YEAR 4 TOTAL
Total costsyear (USD) 3061 2611 2611 2611 10896
AVERAGE PRODUCTION (TON)YEAR 45 45 45 45 18
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 9000 9000 9000 9000 36000
NET PROFIT 5938 6388 6388 6388 25103
OF PROFIT 1939 2446 2446 2446 2303
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 3 3 3 3 12
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 6000 6000 6000 6000 24000
NET PROFIT 2938333 3388333 3388333 3388333 1310333
OF PROFIT 959 1297 1297 1297 1202
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 2 2 2 2 8
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME (USD) 4000 4000 4000 4000 16000
NET PROFIT (USD) 9383333 1388333 1388333 1388333 5103333
OF PROFIT 306 531 531 531 468
From the current study we can notice that the major
costs are the field rent (5000 LE = 833$) and the
starter transplants (8000 LE = 1333$) From this
point to reduce the production costs it is suggested
to focus on producing stevia in the new reclaimed
lands which will lead to reduce the rent cost from
30 to 50 Also a system for producing stevia
seedlings (nurseries) to use as a starter transplants
should be introduced to the producers as a way to
reduce the starter material costs more than 50
One more advantage of cultivating stevia in new
reclaimed lands is the easy use of mechanization
and for setting up modified irrigation systems and
agriculture practices suitable for stevia
bull Stevia is an obligate short day plant with a critical day length of about 11-13 h
bull Long‐days delay flowering and increase stevasoid accumulation Extensive variability within populations for day length sensitivity has been reported
Stevia belongs to
Class Magnoliopsida (Dicotyledons)
Order Asterales
Family Asteraceae or Compositae
Stevia rebaudiana has many commonly
known Synonyms Sweet herb sweet
leaf sugar leaf and honey leaf or simply
Stevia
(مثل عباد الشمس) العائله المركبه
(و زيادة فترات النهار تؤخر االزهار و تسيد من ترجمع االستيفاسىيد( ساعه نهار 13-11)من نباتات النهار القصير
bull The RootThe root of stevia plant is fibrous filamentary
and perennial abundantly branched and
strain distributed near the surface and is
the only organ of the plant that does not
contain stevioside Stem
Root
Foliage
جذور نبات االستيفيا ليفيه فتيلية والمعمرة متشعبه و موزعه بالقرب من سطح
التربه وهي الجهاز الوحيد من النبات الذي ال يحتوي علي مادة االستيفاسويد
المحليه
bull The stemThe stem is annual sub-woody more or less
pubescent with a tendency to lean is more
or less branched During early development
does not have branches becoming
multicaule after the first growing season
coming to produce up to 20 stems in 3 to 4
years Under optimal conditions the stem
can reach five feet in height
Stem
Root
Foliage
bull The leafThe leaves are oval or elliptic lanceolate
small simple toothed edge or margin
sometimes in whorls somewhat hairy
The leaf is the organ with the highest
content of sweetener
Stem
Root
Foliage
ورقه بسيطة بيضاويه صغيره مسننه
bull The FlowersStevia classified as a short-day plant
placing the critical photoperiod at
12 to 13 hours depending on the
ecotype
Flowers are hermaphrodite
small whitish in terminal or axillary short
chapters grouped in corymbose panicles
االزهار صغيره خنثي بيضاء في مجاميع
The hermaphrodite flower is small whitish in
terminal or axillary short chapters grouped in
corymbose panicles A plant takes more than
a month to produce all their flowers The
plant is self-incompatible sporophytic type
and classified as obligatory
apomictic Pollination is by insects
Compound name R1 R2
Stevioside β-Glc β-Glc-β-Glc(2rarr1)
RebaudiosideA β-Glc β-Glc-β-Glc
I
β-Glc (3rarr1)
Rebaudioside C β-Glcβ-Glc -α-Rha(2rarr1)
I
β-Glc (3rarr 1)
Dulcoside A β-Glc β-Glc - α -Rha(2rarrl)
Rubusoside β-Glc β-Glc
Steviolbioside H β-Glc - β-Glc (2rarr1)
RebaudiosideB H β-Glc - β-Glc (2rarr1)
I
β-Glc (3rarr1)
Steviol (RI = R2 = H) is the aglycone of the steviol glycosides Glc and Rha
represent respectively glucose and rhamnose sugar moieties
The seven specific steviol glycosides
The component glycosides
of particular interest for
stevia leaf sweetening
property are stevioside and
Reb A
Powdered Stevia extracts
with 85ndash97 percent Sweet
glycosides (200-300 times
sweeter than sugar
(Sucrose)
Always Keep In
Mind We are
cultivating Stevia
for its leaves
bull A variety of soil types ranging from course
textured sands to well drained loamsare suitable
for growing stevia
but not heavy clay or poorly drained sites
bull Stevia requires very good drainage system
bull Stevia can be grown on a wide range of soil with
pH range from 6 up to 9
bull soil should not be saline
bull Soils with very heavy clay content or high pH
should also be treated or avoided
Before planting each and every
soil sample should be properly
tested and analyzed by an expert
Soil type
bull Climate bull The plant is climatically suited to climates from
temperate to sub-tropical where temperatures
range between 21-43degC with an average of
24degC
bull During the growing season it
seems to thrive in a temperature
range of 15 degC to 34degC provided all
input resources and good
management practices are
incorporated
bull Plant Varieties
bull There are many of Stevia rebaudiana
varieties developed all around the world
These varieties have been developed
for different climate requirements and
Stevioside and rebaudioside content
bull The minimum market requirement of
Stevioside and rebaudioside is 9
bull The grower should select proper
varieties with adequate guarantees from
the planting material suppliers about
minimum assured stevioside and
rebaudioside contents
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
The global market for non-sugar sweeteners in 2011 was at about $92
billion and is expected to reach $93 billion in 2012 The market is expected
to rise at a compound annual growth rate (CAGR) of 11 and reach nearly
$99 billion by 2016 The North American market is estimated to reach
nearly $59 billion in 2012 and The European market represents the second
largest market category with the expected sale of nearly $19 billion in
2012
0
1000
2000
3000
4000
5000
6000
2009 2010 2011 2016
North america Europe Rest of the world
Millio
n U
SD
Sited from httpwwwprlogorg11726488-global-markets-for-non-sugar-sweetenershtml
Since the Stevia extract Rebaudioside A (Reb-A) received
GRAS approval by the US Food and Drug Administration (FDA)
on 17 December 2008 and the approval of the European
Commission on 2 December 2011 there have been several
hundred product launches by food and beverage companies
Steviarsquos status as a global ingredient was secured
with its incorporation into leading beverage
brands manufactured by Coca-Cola and Pepsi
companies in 2009
Now the worldrsquos largest natural
sweetness agent market opened the
door to the Stevia extract of Stevia
extract exports entering the
accelerated growth period
EUROPE9
SOUTH AMERICA
24
NORTH AMERICA
30
ASIA PACIFIC36
OTHERS1
Sited from
httpissuucomzenith_internationaldocsstevia_report_sample
Zenith forecasts that the global market for stevia will reach 11000 metric
tons by 2014 equivalent to US$825 million by value
0
05
1
15
2
25
3
35
2006 2007 2008 2009 2010
(X 1000 ton)
Sited from httpissuucomzenith_internationaldocsstevia_report_sample
One Feddan of stevia produce 4 to 6 tonsyear
(3 harvests) of Dry Leaves
The International price of dry leaves (ge 10
stevisoid) range from 2 to 4 kg USD
Stevia harvest 3 times a year under Egyptian
conditions
International price of 97 stevasoid range from
100 to 150 USDkg
Stevia stay in the field for several years (3 to 5)
years
YEAR 1 YEAR 2 YEAR 3 YEAR 4 TOTAL
Total costsyear (USD) 3061 2611 2611 2611 10896
AVERAGE PRODUCTION (TON)YEAR 45 45 45 45 18
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 9000 9000 9000 9000 36000
NET PROFIT 5938 6388 6388 6388 25103
OF PROFIT 1939 2446 2446 2446 2303
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 3 3 3 3 12
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 6000 6000 6000 6000 24000
NET PROFIT 2938333 3388333 3388333 3388333 1310333
OF PROFIT 959 1297 1297 1297 1202
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 2 2 2 2 8
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME (USD) 4000 4000 4000 4000 16000
NET PROFIT (USD) 9383333 1388333 1388333 1388333 5103333
OF PROFIT 306 531 531 531 468
From the current study we can notice that the major
costs are the field rent (5000 LE = 833$) and the
starter transplants (8000 LE = 1333$) From this
point to reduce the production costs it is suggested
to focus on producing stevia in the new reclaimed
lands which will lead to reduce the rent cost from
30 to 50 Also a system for producing stevia
seedlings (nurseries) to use as a starter transplants
should be introduced to the producers as a way to
reduce the starter material costs more than 50
One more advantage of cultivating stevia in new
reclaimed lands is the easy use of mechanization
and for setting up modified irrigation systems and
agriculture practices suitable for stevia
bull Stevia is an obligate short day plant with a critical day length of about 11-13 h
bull Long‐days delay flowering and increase stevasoid accumulation Extensive variability within populations for day length sensitivity has been reported
Stevia belongs to
Class Magnoliopsida (Dicotyledons)
Order Asterales
Family Asteraceae or Compositae
Stevia rebaudiana has many commonly
known Synonyms Sweet herb sweet
leaf sugar leaf and honey leaf or simply
Stevia
(مثل عباد الشمس) العائله المركبه
(و زيادة فترات النهار تؤخر االزهار و تسيد من ترجمع االستيفاسىيد( ساعه نهار 13-11)من نباتات النهار القصير
bull The RootThe root of stevia plant is fibrous filamentary
and perennial abundantly branched and
strain distributed near the surface and is
the only organ of the plant that does not
contain stevioside Stem
Root
Foliage
جذور نبات االستيفيا ليفيه فتيلية والمعمرة متشعبه و موزعه بالقرب من سطح
التربه وهي الجهاز الوحيد من النبات الذي ال يحتوي علي مادة االستيفاسويد
المحليه
bull The stemThe stem is annual sub-woody more or less
pubescent with a tendency to lean is more
or less branched During early development
does not have branches becoming
multicaule after the first growing season
coming to produce up to 20 stems in 3 to 4
years Under optimal conditions the stem
can reach five feet in height
Stem
Root
Foliage
bull The leafThe leaves are oval or elliptic lanceolate
small simple toothed edge or margin
sometimes in whorls somewhat hairy
The leaf is the organ with the highest
content of sweetener
Stem
Root
Foliage
ورقه بسيطة بيضاويه صغيره مسننه
bull The FlowersStevia classified as a short-day plant
placing the critical photoperiod at
12 to 13 hours depending on the
ecotype
Flowers are hermaphrodite
small whitish in terminal or axillary short
chapters grouped in corymbose panicles
االزهار صغيره خنثي بيضاء في مجاميع
The hermaphrodite flower is small whitish in
terminal or axillary short chapters grouped in
corymbose panicles A plant takes more than
a month to produce all their flowers The
plant is self-incompatible sporophytic type
and classified as obligatory
apomictic Pollination is by insects
Compound name R1 R2
Stevioside β-Glc β-Glc-β-Glc(2rarr1)
RebaudiosideA β-Glc β-Glc-β-Glc
I
β-Glc (3rarr1)
Rebaudioside C β-Glcβ-Glc -α-Rha(2rarr1)
I
β-Glc (3rarr 1)
Dulcoside A β-Glc β-Glc - α -Rha(2rarrl)
Rubusoside β-Glc β-Glc
Steviolbioside H β-Glc - β-Glc (2rarr1)
RebaudiosideB H β-Glc - β-Glc (2rarr1)
I
β-Glc (3rarr1)
Steviol (RI = R2 = H) is the aglycone of the steviol glycosides Glc and Rha
represent respectively glucose and rhamnose sugar moieties
The seven specific steviol glycosides
The component glycosides
of particular interest for
stevia leaf sweetening
property are stevioside and
Reb A
Powdered Stevia extracts
with 85ndash97 percent Sweet
glycosides (200-300 times
sweeter than sugar
(Sucrose)
Always Keep In
Mind We are
cultivating Stevia
for its leaves
bull A variety of soil types ranging from course
textured sands to well drained loamsare suitable
for growing stevia
but not heavy clay or poorly drained sites
bull Stevia requires very good drainage system
bull Stevia can be grown on a wide range of soil with
pH range from 6 up to 9
bull soil should not be saline
bull Soils with very heavy clay content or high pH
should also be treated or avoided
Before planting each and every
soil sample should be properly
tested and analyzed by an expert
Soil type
bull Climate bull The plant is climatically suited to climates from
temperate to sub-tropical where temperatures
range between 21-43degC with an average of
24degC
bull During the growing season it
seems to thrive in a temperature
range of 15 degC to 34degC provided all
input resources and good
management practices are
incorporated
bull Plant Varieties
bull There are many of Stevia rebaudiana
varieties developed all around the world
These varieties have been developed
for different climate requirements and
Stevioside and rebaudioside content
bull The minimum market requirement of
Stevioside and rebaudioside is 9
bull The grower should select proper
varieties with adequate guarantees from
the planting material suppliers about
minimum assured stevioside and
rebaudioside contents
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
Since the Stevia extract Rebaudioside A (Reb-A) received
GRAS approval by the US Food and Drug Administration (FDA)
on 17 December 2008 and the approval of the European
Commission on 2 December 2011 there have been several
hundred product launches by food and beverage companies
Steviarsquos status as a global ingredient was secured
with its incorporation into leading beverage
brands manufactured by Coca-Cola and Pepsi
companies in 2009
Now the worldrsquos largest natural
sweetness agent market opened the
door to the Stevia extract of Stevia
extract exports entering the
accelerated growth period
EUROPE9
SOUTH AMERICA
24
NORTH AMERICA
30
ASIA PACIFIC36
OTHERS1
Sited from
httpissuucomzenith_internationaldocsstevia_report_sample
Zenith forecasts that the global market for stevia will reach 11000 metric
tons by 2014 equivalent to US$825 million by value
0
05
1
15
2
25
3
35
2006 2007 2008 2009 2010
(X 1000 ton)
Sited from httpissuucomzenith_internationaldocsstevia_report_sample
One Feddan of stevia produce 4 to 6 tonsyear
(3 harvests) of Dry Leaves
The International price of dry leaves (ge 10
stevisoid) range from 2 to 4 kg USD
Stevia harvest 3 times a year under Egyptian
conditions
International price of 97 stevasoid range from
100 to 150 USDkg
Stevia stay in the field for several years (3 to 5)
years
YEAR 1 YEAR 2 YEAR 3 YEAR 4 TOTAL
Total costsyear (USD) 3061 2611 2611 2611 10896
AVERAGE PRODUCTION (TON)YEAR 45 45 45 45 18
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 9000 9000 9000 9000 36000
NET PROFIT 5938 6388 6388 6388 25103
OF PROFIT 1939 2446 2446 2446 2303
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 3 3 3 3 12
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 6000 6000 6000 6000 24000
NET PROFIT 2938333 3388333 3388333 3388333 1310333
OF PROFIT 959 1297 1297 1297 1202
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 2 2 2 2 8
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME (USD) 4000 4000 4000 4000 16000
NET PROFIT (USD) 9383333 1388333 1388333 1388333 5103333
OF PROFIT 306 531 531 531 468
From the current study we can notice that the major
costs are the field rent (5000 LE = 833$) and the
starter transplants (8000 LE = 1333$) From this
point to reduce the production costs it is suggested
to focus on producing stevia in the new reclaimed
lands which will lead to reduce the rent cost from
30 to 50 Also a system for producing stevia
seedlings (nurseries) to use as a starter transplants
should be introduced to the producers as a way to
reduce the starter material costs more than 50
One more advantage of cultivating stevia in new
reclaimed lands is the easy use of mechanization
and for setting up modified irrigation systems and
agriculture practices suitable for stevia
bull Stevia is an obligate short day plant with a critical day length of about 11-13 h
bull Long‐days delay flowering and increase stevasoid accumulation Extensive variability within populations for day length sensitivity has been reported
Stevia belongs to
Class Magnoliopsida (Dicotyledons)
Order Asterales
Family Asteraceae or Compositae
Stevia rebaudiana has many commonly
known Synonyms Sweet herb sweet
leaf sugar leaf and honey leaf or simply
Stevia
(مثل عباد الشمس) العائله المركبه
(و زيادة فترات النهار تؤخر االزهار و تسيد من ترجمع االستيفاسىيد( ساعه نهار 13-11)من نباتات النهار القصير
bull The RootThe root of stevia plant is fibrous filamentary
and perennial abundantly branched and
strain distributed near the surface and is
the only organ of the plant that does not
contain stevioside Stem
Root
Foliage
جذور نبات االستيفيا ليفيه فتيلية والمعمرة متشعبه و موزعه بالقرب من سطح
التربه وهي الجهاز الوحيد من النبات الذي ال يحتوي علي مادة االستيفاسويد
المحليه
bull The stemThe stem is annual sub-woody more or less
pubescent with a tendency to lean is more
or less branched During early development
does not have branches becoming
multicaule after the first growing season
coming to produce up to 20 stems in 3 to 4
years Under optimal conditions the stem
can reach five feet in height
Stem
Root
Foliage
bull The leafThe leaves are oval or elliptic lanceolate
small simple toothed edge or margin
sometimes in whorls somewhat hairy
The leaf is the organ with the highest
content of sweetener
Stem
Root
Foliage
ورقه بسيطة بيضاويه صغيره مسننه
bull The FlowersStevia classified as a short-day plant
placing the critical photoperiod at
12 to 13 hours depending on the
ecotype
Flowers are hermaphrodite
small whitish in terminal or axillary short
chapters grouped in corymbose panicles
االزهار صغيره خنثي بيضاء في مجاميع
The hermaphrodite flower is small whitish in
terminal or axillary short chapters grouped in
corymbose panicles A plant takes more than
a month to produce all their flowers The
plant is self-incompatible sporophytic type
and classified as obligatory
apomictic Pollination is by insects
Compound name R1 R2
Stevioside β-Glc β-Glc-β-Glc(2rarr1)
RebaudiosideA β-Glc β-Glc-β-Glc
I
β-Glc (3rarr1)
Rebaudioside C β-Glcβ-Glc -α-Rha(2rarr1)
I
β-Glc (3rarr 1)
Dulcoside A β-Glc β-Glc - α -Rha(2rarrl)
Rubusoside β-Glc β-Glc
Steviolbioside H β-Glc - β-Glc (2rarr1)
RebaudiosideB H β-Glc - β-Glc (2rarr1)
I
β-Glc (3rarr1)
Steviol (RI = R2 = H) is the aglycone of the steviol glycosides Glc and Rha
represent respectively glucose and rhamnose sugar moieties
The seven specific steviol glycosides
The component glycosides
of particular interest for
stevia leaf sweetening
property are stevioside and
Reb A
Powdered Stevia extracts
with 85ndash97 percent Sweet
glycosides (200-300 times
sweeter than sugar
(Sucrose)
Always Keep In
Mind We are
cultivating Stevia
for its leaves
bull A variety of soil types ranging from course
textured sands to well drained loamsare suitable
for growing stevia
but not heavy clay or poorly drained sites
bull Stevia requires very good drainage system
bull Stevia can be grown on a wide range of soil with
pH range from 6 up to 9
bull soil should not be saline
bull Soils with very heavy clay content or high pH
should also be treated or avoided
Before planting each and every
soil sample should be properly
tested and analyzed by an expert
Soil type
bull Climate bull The plant is climatically suited to climates from
temperate to sub-tropical where temperatures
range between 21-43degC with an average of
24degC
bull During the growing season it
seems to thrive in a temperature
range of 15 degC to 34degC provided all
input resources and good
management practices are
incorporated
bull Plant Varieties
bull There are many of Stevia rebaudiana
varieties developed all around the world
These varieties have been developed
for different climate requirements and
Stevioside and rebaudioside content
bull The minimum market requirement of
Stevioside and rebaudioside is 9
bull The grower should select proper
varieties with adequate guarantees from
the planting material suppliers about
minimum assured stevioside and
rebaudioside contents
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
Steviarsquos status as a global ingredient was secured
with its incorporation into leading beverage
brands manufactured by Coca-Cola and Pepsi
companies in 2009
Now the worldrsquos largest natural
sweetness agent market opened the
door to the Stevia extract of Stevia
extract exports entering the
accelerated growth period
EUROPE9
SOUTH AMERICA
24
NORTH AMERICA
30
ASIA PACIFIC36
OTHERS1
Sited from
httpissuucomzenith_internationaldocsstevia_report_sample
Zenith forecasts that the global market for stevia will reach 11000 metric
tons by 2014 equivalent to US$825 million by value
0
05
1
15
2
25
3
35
2006 2007 2008 2009 2010
(X 1000 ton)
Sited from httpissuucomzenith_internationaldocsstevia_report_sample
One Feddan of stevia produce 4 to 6 tonsyear
(3 harvests) of Dry Leaves
The International price of dry leaves (ge 10
stevisoid) range from 2 to 4 kg USD
Stevia harvest 3 times a year under Egyptian
conditions
International price of 97 stevasoid range from
100 to 150 USDkg
Stevia stay in the field for several years (3 to 5)
years
YEAR 1 YEAR 2 YEAR 3 YEAR 4 TOTAL
Total costsyear (USD) 3061 2611 2611 2611 10896
AVERAGE PRODUCTION (TON)YEAR 45 45 45 45 18
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 9000 9000 9000 9000 36000
NET PROFIT 5938 6388 6388 6388 25103
OF PROFIT 1939 2446 2446 2446 2303
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 3 3 3 3 12
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 6000 6000 6000 6000 24000
NET PROFIT 2938333 3388333 3388333 3388333 1310333
OF PROFIT 959 1297 1297 1297 1202
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 2 2 2 2 8
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME (USD) 4000 4000 4000 4000 16000
NET PROFIT (USD) 9383333 1388333 1388333 1388333 5103333
OF PROFIT 306 531 531 531 468
From the current study we can notice that the major
costs are the field rent (5000 LE = 833$) and the
starter transplants (8000 LE = 1333$) From this
point to reduce the production costs it is suggested
to focus on producing stevia in the new reclaimed
lands which will lead to reduce the rent cost from
30 to 50 Also a system for producing stevia
seedlings (nurseries) to use as a starter transplants
should be introduced to the producers as a way to
reduce the starter material costs more than 50
One more advantage of cultivating stevia in new
reclaimed lands is the easy use of mechanization
and for setting up modified irrigation systems and
agriculture practices suitable for stevia
bull Stevia is an obligate short day plant with a critical day length of about 11-13 h
bull Long‐days delay flowering and increase stevasoid accumulation Extensive variability within populations for day length sensitivity has been reported
Stevia belongs to
Class Magnoliopsida (Dicotyledons)
Order Asterales
Family Asteraceae or Compositae
Stevia rebaudiana has many commonly
known Synonyms Sweet herb sweet
leaf sugar leaf and honey leaf or simply
Stevia
(مثل عباد الشمس) العائله المركبه
(و زيادة فترات النهار تؤخر االزهار و تسيد من ترجمع االستيفاسىيد( ساعه نهار 13-11)من نباتات النهار القصير
bull The RootThe root of stevia plant is fibrous filamentary
and perennial abundantly branched and
strain distributed near the surface and is
the only organ of the plant that does not
contain stevioside Stem
Root
Foliage
جذور نبات االستيفيا ليفيه فتيلية والمعمرة متشعبه و موزعه بالقرب من سطح
التربه وهي الجهاز الوحيد من النبات الذي ال يحتوي علي مادة االستيفاسويد
المحليه
bull The stemThe stem is annual sub-woody more or less
pubescent with a tendency to lean is more
or less branched During early development
does not have branches becoming
multicaule after the first growing season
coming to produce up to 20 stems in 3 to 4
years Under optimal conditions the stem
can reach five feet in height
Stem
Root
Foliage
bull The leafThe leaves are oval or elliptic lanceolate
small simple toothed edge or margin
sometimes in whorls somewhat hairy
The leaf is the organ with the highest
content of sweetener
Stem
Root
Foliage
ورقه بسيطة بيضاويه صغيره مسننه
bull The FlowersStevia classified as a short-day plant
placing the critical photoperiod at
12 to 13 hours depending on the
ecotype
Flowers are hermaphrodite
small whitish in terminal or axillary short
chapters grouped in corymbose panicles
االزهار صغيره خنثي بيضاء في مجاميع
The hermaphrodite flower is small whitish in
terminal or axillary short chapters grouped in
corymbose panicles A plant takes more than
a month to produce all their flowers The
plant is self-incompatible sporophytic type
and classified as obligatory
apomictic Pollination is by insects
Compound name R1 R2
Stevioside β-Glc β-Glc-β-Glc(2rarr1)
RebaudiosideA β-Glc β-Glc-β-Glc
I
β-Glc (3rarr1)
Rebaudioside C β-Glcβ-Glc -α-Rha(2rarr1)
I
β-Glc (3rarr 1)
Dulcoside A β-Glc β-Glc - α -Rha(2rarrl)
Rubusoside β-Glc β-Glc
Steviolbioside H β-Glc - β-Glc (2rarr1)
RebaudiosideB H β-Glc - β-Glc (2rarr1)
I
β-Glc (3rarr1)
Steviol (RI = R2 = H) is the aglycone of the steviol glycosides Glc and Rha
represent respectively glucose and rhamnose sugar moieties
The seven specific steviol glycosides
The component glycosides
of particular interest for
stevia leaf sweetening
property are stevioside and
Reb A
Powdered Stevia extracts
with 85ndash97 percent Sweet
glycosides (200-300 times
sweeter than sugar
(Sucrose)
Always Keep In
Mind We are
cultivating Stevia
for its leaves
bull A variety of soil types ranging from course
textured sands to well drained loamsare suitable
for growing stevia
but not heavy clay or poorly drained sites
bull Stevia requires very good drainage system
bull Stevia can be grown on a wide range of soil with
pH range from 6 up to 9
bull soil should not be saline
bull Soils with very heavy clay content or high pH
should also be treated or avoided
Before planting each and every
soil sample should be properly
tested and analyzed by an expert
Soil type
bull Climate bull The plant is climatically suited to climates from
temperate to sub-tropical where temperatures
range between 21-43degC with an average of
24degC
bull During the growing season it
seems to thrive in a temperature
range of 15 degC to 34degC provided all
input resources and good
management practices are
incorporated
bull Plant Varieties
bull There are many of Stevia rebaudiana
varieties developed all around the world
These varieties have been developed
for different climate requirements and
Stevioside and rebaudioside content
bull The minimum market requirement of
Stevioside and rebaudioside is 9
bull The grower should select proper
varieties with adequate guarantees from
the planting material suppliers about
minimum assured stevioside and
rebaudioside contents
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
EUROPE9
SOUTH AMERICA
24
NORTH AMERICA
30
ASIA PACIFIC36
OTHERS1
Sited from
httpissuucomzenith_internationaldocsstevia_report_sample
Zenith forecasts that the global market for stevia will reach 11000 metric
tons by 2014 equivalent to US$825 million by value
0
05
1
15
2
25
3
35
2006 2007 2008 2009 2010
(X 1000 ton)
Sited from httpissuucomzenith_internationaldocsstevia_report_sample
One Feddan of stevia produce 4 to 6 tonsyear
(3 harvests) of Dry Leaves
The International price of dry leaves (ge 10
stevisoid) range from 2 to 4 kg USD
Stevia harvest 3 times a year under Egyptian
conditions
International price of 97 stevasoid range from
100 to 150 USDkg
Stevia stay in the field for several years (3 to 5)
years
YEAR 1 YEAR 2 YEAR 3 YEAR 4 TOTAL
Total costsyear (USD) 3061 2611 2611 2611 10896
AVERAGE PRODUCTION (TON)YEAR 45 45 45 45 18
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 9000 9000 9000 9000 36000
NET PROFIT 5938 6388 6388 6388 25103
OF PROFIT 1939 2446 2446 2446 2303
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 3 3 3 3 12
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 6000 6000 6000 6000 24000
NET PROFIT 2938333 3388333 3388333 3388333 1310333
OF PROFIT 959 1297 1297 1297 1202
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 2 2 2 2 8
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME (USD) 4000 4000 4000 4000 16000
NET PROFIT (USD) 9383333 1388333 1388333 1388333 5103333
OF PROFIT 306 531 531 531 468
From the current study we can notice that the major
costs are the field rent (5000 LE = 833$) and the
starter transplants (8000 LE = 1333$) From this
point to reduce the production costs it is suggested
to focus on producing stevia in the new reclaimed
lands which will lead to reduce the rent cost from
30 to 50 Also a system for producing stevia
seedlings (nurseries) to use as a starter transplants
should be introduced to the producers as a way to
reduce the starter material costs more than 50
One more advantage of cultivating stevia in new
reclaimed lands is the easy use of mechanization
and for setting up modified irrigation systems and
agriculture practices suitable for stevia
bull Stevia is an obligate short day plant with a critical day length of about 11-13 h
bull Long‐days delay flowering and increase stevasoid accumulation Extensive variability within populations for day length sensitivity has been reported
Stevia belongs to
Class Magnoliopsida (Dicotyledons)
Order Asterales
Family Asteraceae or Compositae
Stevia rebaudiana has many commonly
known Synonyms Sweet herb sweet
leaf sugar leaf and honey leaf or simply
Stevia
(مثل عباد الشمس) العائله المركبه
(و زيادة فترات النهار تؤخر االزهار و تسيد من ترجمع االستيفاسىيد( ساعه نهار 13-11)من نباتات النهار القصير
bull The RootThe root of stevia plant is fibrous filamentary
and perennial abundantly branched and
strain distributed near the surface and is
the only organ of the plant that does not
contain stevioside Stem
Root
Foliage
جذور نبات االستيفيا ليفيه فتيلية والمعمرة متشعبه و موزعه بالقرب من سطح
التربه وهي الجهاز الوحيد من النبات الذي ال يحتوي علي مادة االستيفاسويد
المحليه
bull The stemThe stem is annual sub-woody more or less
pubescent with a tendency to lean is more
or less branched During early development
does not have branches becoming
multicaule after the first growing season
coming to produce up to 20 stems in 3 to 4
years Under optimal conditions the stem
can reach five feet in height
Stem
Root
Foliage
bull The leafThe leaves are oval or elliptic lanceolate
small simple toothed edge or margin
sometimes in whorls somewhat hairy
The leaf is the organ with the highest
content of sweetener
Stem
Root
Foliage
ورقه بسيطة بيضاويه صغيره مسننه
bull The FlowersStevia classified as a short-day plant
placing the critical photoperiod at
12 to 13 hours depending on the
ecotype
Flowers are hermaphrodite
small whitish in terminal or axillary short
chapters grouped in corymbose panicles
االزهار صغيره خنثي بيضاء في مجاميع
The hermaphrodite flower is small whitish in
terminal or axillary short chapters grouped in
corymbose panicles A plant takes more than
a month to produce all their flowers The
plant is self-incompatible sporophytic type
and classified as obligatory
apomictic Pollination is by insects
Compound name R1 R2
Stevioside β-Glc β-Glc-β-Glc(2rarr1)
RebaudiosideA β-Glc β-Glc-β-Glc
I
β-Glc (3rarr1)
Rebaudioside C β-Glcβ-Glc -α-Rha(2rarr1)
I
β-Glc (3rarr 1)
Dulcoside A β-Glc β-Glc - α -Rha(2rarrl)
Rubusoside β-Glc β-Glc
Steviolbioside H β-Glc - β-Glc (2rarr1)
RebaudiosideB H β-Glc - β-Glc (2rarr1)
I
β-Glc (3rarr1)
Steviol (RI = R2 = H) is the aglycone of the steviol glycosides Glc and Rha
represent respectively glucose and rhamnose sugar moieties
The seven specific steviol glycosides
The component glycosides
of particular interest for
stevia leaf sweetening
property are stevioside and
Reb A
Powdered Stevia extracts
with 85ndash97 percent Sweet
glycosides (200-300 times
sweeter than sugar
(Sucrose)
Always Keep In
Mind We are
cultivating Stevia
for its leaves
bull A variety of soil types ranging from course
textured sands to well drained loamsare suitable
for growing stevia
but not heavy clay or poorly drained sites
bull Stevia requires very good drainage system
bull Stevia can be grown on a wide range of soil with
pH range from 6 up to 9
bull soil should not be saline
bull Soils with very heavy clay content or high pH
should also be treated or avoided
Before planting each and every
soil sample should be properly
tested and analyzed by an expert
Soil type
bull Climate bull The plant is climatically suited to climates from
temperate to sub-tropical where temperatures
range between 21-43degC with an average of
24degC
bull During the growing season it
seems to thrive in a temperature
range of 15 degC to 34degC provided all
input resources and good
management practices are
incorporated
bull Plant Varieties
bull There are many of Stevia rebaudiana
varieties developed all around the world
These varieties have been developed
for different climate requirements and
Stevioside and rebaudioside content
bull The minimum market requirement of
Stevioside and rebaudioside is 9
bull The grower should select proper
varieties with adequate guarantees from
the planting material suppliers about
minimum assured stevioside and
rebaudioside contents
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
Zenith forecasts that the global market for stevia will reach 11000 metric
tons by 2014 equivalent to US$825 million by value
0
05
1
15
2
25
3
35
2006 2007 2008 2009 2010
(X 1000 ton)
Sited from httpissuucomzenith_internationaldocsstevia_report_sample
One Feddan of stevia produce 4 to 6 tonsyear
(3 harvests) of Dry Leaves
The International price of dry leaves (ge 10
stevisoid) range from 2 to 4 kg USD
Stevia harvest 3 times a year under Egyptian
conditions
International price of 97 stevasoid range from
100 to 150 USDkg
Stevia stay in the field for several years (3 to 5)
years
YEAR 1 YEAR 2 YEAR 3 YEAR 4 TOTAL
Total costsyear (USD) 3061 2611 2611 2611 10896
AVERAGE PRODUCTION (TON)YEAR 45 45 45 45 18
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 9000 9000 9000 9000 36000
NET PROFIT 5938 6388 6388 6388 25103
OF PROFIT 1939 2446 2446 2446 2303
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 3 3 3 3 12
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 6000 6000 6000 6000 24000
NET PROFIT 2938333 3388333 3388333 3388333 1310333
OF PROFIT 959 1297 1297 1297 1202
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 2 2 2 2 8
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME (USD) 4000 4000 4000 4000 16000
NET PROFIT (USD) 9383333 1388333 1388333 1388333 5103333
OF PROFIT 306 531 531 531 468
From the current study we can notice that the major
costs are the field rent (5000 LE = 833$) and the
starter transplants (8000 LE = 1333$) From this
point to reduce the production costs it is suggested
to focus on producing stevia in the new reclaimed
lands which will lead to reduce the rent cost from
30 to 50 Also a system for producing stevia
seedlings (nurseries) to use as a starter transplants
should be introduced to the producers as a way to
reduce the starter material costs more than 50
One more advantage of cultivating stevia in new
reclaimed lands is the easy use of mechanization
and for setting up modified irrigation systems and
agriculture practices suitable for stevia
bull Stevia is an obligate short day plant with a critical day length of about 11-13 h
bull Long‐days delay flowering and increase stevasoid accumulation Extensive variability within populations for day length sensitivity has been reported
Stevia belongs to
Class Magnoliopsida (Dicotyledons)
Order Asterales
Family Asteraceae or Compositae
Stevia rebaudiana has many commonly
known Synonyms Sweet herb sweet
leaf sugar leaf and honey leaf or simply
Stevia
(مثل عباد الشمس) العائله المركبه
(و زيادة فترات النهار تؤخر االزهار و تسيد من ترجمع االستيفاسىيد( ساعه نهار 13-11)من نباتات النهار القصير
bull The RootThe root of stevia plant is fibrous filamentary
and perennial abundantly branched and
strain distributed near the surface and is
the only organ of the plant that does not
contain stevioside Stem
Root
Foliage
جذور نبات االستيفيا ليفيه فتيلية والمعمرة متشعبه و موزعه بالقرب من سطح
التربه وهي الجهاز الوحيد من النبات الذي ال يحتوي علي مادة االستيفاسويد
المحليه
bull The stemThe stem is annual sub-woody more or less
pubescent with a tendency to lean is more
or less branched During early development
does not have branches becoming
multicaule after the first growing season
coming to produce up to 20 stems in 3 to 4
years Under optimal conditions the stem
can reach five feet in height
Stem
Root
Foliage
bull The leafThe leaves are oval or elliptic lanceolate
small simple toothed edge or margin
sometimes in whorls somewhat hairy
The leaf is the organ with the highest
content of sweetener
Stem
Root
Foliage
ورقه بسيطة بيضاويه صغيره مسننه
bull The FlowersStevia classified as a short-day plant
placing the critical photoperiod at
12 to 13 hours depending on the
ecotype
Flowers are hermaphrodite
small whitish in terminal or axillary short
chapters grouped in corymbose panicles
االزهار صغيره خنثي بيضاء في مجاميع
The hermaphrodite flower is small whitish in
terminal or axillary short chapters grouped in
corymbose panicles A plant takes more than
a month to produce all their flowers The
plant is self-incompatible sporophytic type
and classified as obligatory
apomictic Pollination is by insects
Compound name R1 R2
Stevioside β-Glc β-Glc-β-Glc(2rarr1)
RebaudiosideA β-Glc β-Glc-β-Glc
I
β-Glc (3rarr1)
Rebaudioside C β-Glcβ-Glc -α-Rha(2rarr1)
I
β-Glc (3rarr 1)
Dulcoside A β-Glc β-Glc - α -Rha(2rarrl)
Rubusoside β-Glc β-Glc
Steviolbioside H β-Glc - β-Glc (2rarr1)
RebaudiosideB H β-Glc - β-Glc (2rarr1)
I
β-Glc (3rarr1)
Steviol (RI = R2 = H) is the aglycone of the steviol glycosides Glc and Rha
represent respectively glucose and rhamnose sugar moieties
The seven specific steviol glycosides
The component glycosides
of particular interest for
stevia leaf sweetening
property are stevioside and
Reb A
Powdered Stevia extracts
with 85ndash97 percent Sweet
glycosides (200-300 times
sweeter than sugar
(Sucrose)
Always Keep In
Mind We are
cultivating Stevia
for its leaves
bull A variety of soil types ranging from course
textured sands to well drained loamsare suitable
for growing stevia
but not heavy clay or poorly drained sites
bull Stevia requires very good drainage system
bull Stevia can be grown on a wide range of soil with
pH range from 6 up to 9
bull soil should not be saline
bull Soils with very heavy clay content or high pH
should also be treated or avoided
Before planting each and every
soil sample should be properly
tested and analyzed by an expert
Soil type
bull Climate bull The plant is climatically suited to climates from
temperate to sub-tropical where temperatures
range between 21-43degC with an average of
24degC
bull During the growing season it
seems to thrive in a temperature
range of 15 degC to 34degC provided all
input resources and good
management practices are
incorporated
bull Plant Varieties
bull There are many of Stevia rebaudiana
varieties developed all around the world
These varieties have been developed
for different climate requirements and
Stevioside and rebaudioside content
bull The minimum market requirement of
Stevioside and rebaudioside is 9
bull The grower should select proper
varieties with adequate guarantees from
the planting material suppliers about
minimum assured stevioside and
rebaudioside contents
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
One Feddan of stevia produce 4 to 6 tonsyear
(3 harvests) of Dry Leaves
The International price of dry leaves (ge 10
stevisoid) range from 2 to 4 kg USD
Stevia harvest 3 times a year under Egyptian
conditions
International price of 97 stevasoid range from
100 to 150 USDkg
Stevia stay in the field for several years (3 to 5)
years
YEAR 1 YEAR 2 YEAR 3 YEAR 4 TOTAL
Total costsyear (USD) 3061 2611 2611 2611 10896
AVERAGE PRODUCTION (TON)YEAR 45 45 45 45 18
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 9000 9000 9000 9000 36000
NET PROFIT 5938 6388 6388 6388 25103
OF PROFIT 1939 2446 2446 2446 2303
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 3 3 3 3 12
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 6000 6000 6000 6000 24000
NET PROFIT 2938333 3388333 3388333 3388333 1310333
OF PROFIT 959 1297 1297 1297 1202
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 2 2 2 2 8
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME (USD) 4000 4000 4000 4000 16000
NET PROFIT (USD) 9383333 1388333 1388333 1388333 5103333
OF PROFIT 306 531 531 531 468
From the current study we can notice that the major
costs are the field rent (5000 LE = 833$) and the
starter transplants (8000 LE = 1333$) From this
point to reduce the production costs it is suggested
to focus on producing stevia in the new reclaimed
lands which will lead to reduce the rent cost from
30 to 50 Also a system for producing stevia
seedlings (nurseries) to use as a starter transplants
should be introduced to the producers as a way to
reduce the starter material costs more than 50
One more advantage of cultivating stevia in new
reclaimed lands is the easy use of mechanization
and for setting up modified irrigation systems and
agriculture practices suitable for stevia
bull Stevia is an obligate short day plant with a critical day length of about 11-13 h
bull Long‐days delay flowering and increase stevasoid accumulation Extensive variability within populations for day length sensitivity has been reported
Stevia belongs to
Class Magnoliopsida (Dicotyledons)
Order Asterales
Family Asteraceae or Compositae
Stevia rebaudiana has many commonly
known Synonyms Sweet herb sweet
leaf sugar leaf and honey leaf or simply
Stevia
(مثل عباد الشمس) العائله المركبه
(و زيادة فترات النهار تؤخر االزهار و تسيد من ترجمع االستيفاسىيد( ساعه نهار 13-11)من نباتات النهار القصير
bull The RootThe root of stevia plant is fibrous filamentary
and perennial abundantly branched and
strain distributed near the surface and is
the only organ of the plant that does not
contain stevioside Stem
Root
Foliage
جذور نبات االستيفيا ليفيه فتيلية والمعمرة متشعبه و موزعه بالقرب من سطح
التربه وهي الجهاز الوحيد من النبات الذي ال يحتوي علي مادة االستيفاسويد
المحليه
bull The stemThe stem is annual sub-woody more or less
pubescent with a tendency to lean is more
or less branched During early development
does not have branches becoming
multicaule after the first growing season
coming to produce up to 20 stems in 3 to 4
years Under optimal conditions the stem
can reach five feet in height
Stem
Root
Foliage
bull The leafThe leaves are oval or elliptic lanceolate
small simple toothed edge or margin
sometimes in whorls somewhat hairy
The leaf is the organ with the highest
content of sweetener
Stem
Root
Foliage
ورقه بسيطة بيضاويه صغيره مسننه
bull The FlowersStevia classified as a short-day plant
placing the critical photoperiod at
12 to 13 hours depending on the
ecotype
Flowers are hermaphrodite
small whitish in terminal or axillary short
chapters grouped in corymbose panicles
االزهار صغيره خنثي بيضاء في مجاميع
The hermaphrodite flower is small whitish in
terminal or axillary short chapters grouped in
corymbose panicles A plant takes more than
a month to produce all their flowers The
plant is self-incompatible sporophytic type
and classified as obligatory
apomictic Pollination is by insects
Compound name R1 R2
Stevioside β-Glc β-Glc-β-Glc(2rarr1)
RebaudiosideA β-Glc β-Glc-β-Glc
I
β-Glc (3rarr1)
Rebaudioside C β-Glcβ-Glc -α-Rha(2rarr1)
I
β-Glc (3rarr 1)
Dulcoside A β-Glc β-Glc - α -Rha(2rarrl)
Rubusoside β-Glc β-Glc
Steviolbioside H β-Glc - β-Glc (2rarr1)
RebaudiosideB H β-Glc - β-Glc (2rarr1)
I
β-Glc (3rarr1)
Steviol (RI = R2 = H) is the aglycone of the steviol glycosides Glc and Rha
represent respectively glucose and rhamnose sugar moieties
The seven specific steviol glycosides
The component glycosides
of particular interest for
stevia leaf sweetening
property are stevioside and
Reb A
Powdered Stevia extracts
with 85ndash97 percent Sweet
glycosides (200-300 times
sweeter than sugar
(Sucrose)
Always Keep In
Mind We are
cultivating Stevia
for its leaves
bull A variety of soil types ranging from course
textured sands to well drained loamsare suitable
for growing stevia
but not heavy clay or poorly drained sites
bull Stevia requires very good drainage system
bull Stevia can be grown on a wide range of soil with
pH range from 6 up to 9
bull soil should not be saline
bull Soils with very heavy clay content or high pH
should also be treated or avoided
Before planting each and every
soil sample should be properly
tested and analyzed by an expert
Soil type
bull Climate bull The plant is climatically suited to climates from
temperate to sub-tropical where temperatures
range between 21-43degC with an average of
24degC
bull During the growing season it
seems to thrive in a temperature
range of 15 degC to 34degC provided all
input resources and good
management practices are
incorporated
bull Plant Varieties
bull There are many of Stevia rebaudiana
varieties developed all around the world
These varieties have been developed
for different climate requirements and
Stevioside and rebaudioside content
bull The minimum market requirement of
Stevioside and rebaudioside is 9
bull The grower should select proper
varieties with adequate guarantees from
the planting material suppliers about
minimum assured stevioside and
rebaudioside contents
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
YEAR 1 YEAR 2 YEAR 3 YEAR 4 TOTAL
Total costsyear (USD) 3061 2611 2611 2611 10896
AVERAGE PRODUCTION (TON)YEAR 45 45 45 45 18
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 9000 9000 9000 9000 36000
NET PROFIT 5938 6388 6388 6388 25103
OF PROFIT 1939 2446 2446 2446 2303
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 3 3 3 3 12
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME 6000 6000 6000 6000 24000
NET PROFIT 2938333 3388333 3388333 3388333 1310333
OF PROFIT 959 1297 1297 1297 1202
Total costsyear (USD) 3061667 2611667 2611667 2611667 1089667
AVERAGE PRODUCTION (TON)YEAR 2 2 2 2 8
INTERNATIONAL PRICE (USD)TON 2000 2000 2000 2000 2000
TOTAL INCOME (USD) 4000 4000 4000 4000 16000
NET PROFIT (USD) 9383333 1388333 1388333 1388333 5103333
OF PROFIT 306 531 531 531 468
From the current study we can notice that the major
costs are the field rent (5000 LE = 833$) and the
starter transplants (8000 LE = 1333$) From this
point to reduce the production costs it is suggested
to focus on producing stevia in the new reclaimed
lands which will lead to reduce the rent cost from
30 to 50 Also a system for producing stevia
seedlings (nurseries) to use as a starter transplants
should be introduced to the producers as a way to
reduce the starter material costs more than 50
One more advantage of cultivating stevia in new
reclaimed lands is the easy use of mechanization
and for setting up modified irrigation systems and
agriculture practices suitable for stevia
bull Stevia is an obligate short day plant with a critical day length of about 11-13 h
bull Long‐days delay flowering and increase stevasoid accumulation Extensive variability within populations for day length sensitivity has been reported
Stevia belongs to
Class Magnoliopsida (Dicotyledons)
Order Asterales
Family Asteraceae or Compositae
Stevia rebaudiana has many commonly
known Synonyms Sweet herb sweet
leaf sugar leaf and honey leaf or simply
Stevia
(مثل عباد الشمس) العائله المركبه
(و زيادة فترات النهار تؤخر االزهار و تسيد من ترجمع االستيفاسىيد( ساعه نهار 13-11)من نباتات النهار القصير
bull The RootThe root of stevia plant is fibrous filamentary
and perennial abundantly branched and
strain distributed near the surface and is
the only organ of the plant that does not
contain stevioside Stem
Root
Foliage
جذور نبات االستيفيا ليفيه فتيلية والمعمرة متشعبه و موزعه بالقرب من سطح
التربه وهي الجهاز الوحيد من النبات الذي ال يحتوي علي مادة االستيفاسويد
المحليه
bull The stemThe stem is annual sub-woody more or less
pubescent with a tendency to lean is more
or less branched During early development
does not have branches becoming
multicaule after the first growing season
coming to produce up to 20 stems in 3 to 4
years Under optimal conditions the stem
can reach five feet in height
Stem
Root
Foliage
bull The leafThe leaves are oval or elliptic lanceolate
small simple toothed edge or margin
sometimes in whorls somewhat hairy
The leaf is the organ with the highest
content of sweetener
Stem
Root
Foliage
ورقه بسيطة بيضاويه صغيره مسننه
bull The FlowersStevia classified as a short-day plant
placing the critical photoperiod at
12 to 13 hours depending on the
ecotype
Flowers are hermaphrodite
small whitish in terminal or axillary short
chapters grouped in corymbose panicles
االزهار صغيره خنثي بيضاء في مجاميع
The hermaphrodite flower is small whitish in
terminal or axillary short chapters grouped in
corymbose panicles A plant takes more than
a month to produce all their flowers The
plant is self-incompatible sporophytic type
and classified as obligatory
apomictic Pollination is by insects
Compound name R1 R2
Stevioside β-Glc β-Glc-β-Glc(2rarr1)
RebaudiosideA β-Glc β-Glc-β-Glc
I
β-Glc (3rarr1)
Rebaudioside C β-Glcβ-Glc -α-Rha(2rarr1)
I
β-Glc (3rarr 1)
Dulcoside A β-Glc β-Glc - α -Rha(2rarrl)
Rubusoside β-Glc β-Glc
Steviolbioside H β-Glc - β-Glc (2rarr1)
RebaudiosideB H β-Glc - β-Glc (2rarr1)
I
β-Glc (3rarr1)
Steviol (RI = R2 = H) is the aglycone of the steviol glycosides Glc and Rha
represent respectively glucose and rhamnose sugar moieties
The seven specific steviol glycosides
The component glycosides
of particular interest for
stevia leaf sweetening
property are stevioside and
Reb A
Powdered Stevia extracts
with 85ndash97 percent Sweet
glycosides (200-300 times
sweeter than sugar
(Sucrose)
Always Keep In
Mind We are
cultivating Stevia
for its leaves
bull A variety of soil types ranging from course
textured sands to well drained loamsare suitable
for growing stevia
but not heavy clay or poorly drained sites
bull Stevia requires very good drainage system
bull Stevia can be grown on a wide range of soil with
pH range from 6 up to 9
bull soil should not be saline
bull Soils with very heavy clay content or high pH
should also be treated or avoided
Before planting each and every
soil sample should be properly
tested and analyzed by an expert
Soil type
bull Climate bull The plant is climatically suited to climates from
temperate to sub-tropical where temperatures
range between 21-43degC with an average of
24degC
bull During the growing season it
seems to thrive in a temperature
range of 15 degC to 34degC provided all
input resources and good
management practices are
incorporated
bull Plant Varieties
bull There are many of Stevia rebaudiana
varieties developed all around the world
These varieties have been developed
for different climate requirements and
Stevioside and rebaudioside content
bull The minimum market requirement of
Stevioside and rebaudioside is 9
bull The grower should select proper
varieties with adequate guarantees from
the planting material suppliers about
minimum assured stevioside and
rebaudioside contents
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
From the current study we can notice that the major
costs are the field rent (5000 LE = 833$) and the
starter transplants (8000 LE = 1333$) From this
point to reduce the production costs it is suggested
to focus on producing stevia in the new reclaimed
lands which will lead to reduce the rent cost from
30 to 50 Also a system for producing stevia
seedlings (nurseries) to use as a starter transplants
should be introduced to the producers as a way to
reduce the starter material costs more than 50
One more advantage of cultivating stevia in new
reclaimed lands is the easy use of mechanization
and for setting up modified irrigation systems and
agriculture practices suitable for stevia
bull Stevia is an obligate short day plant with a critical day length of about 11-13 h
bull Long‐days delay flowering and increase stevasoid accumulation Extensive variability within populations for day length sensitivity has been reported
Stevia belongs to
Class Magnoliopsida (Dicotyledons)
Order Asterales
Family Asteraceae or Compositae
Stevia rebaudiana has many commonly
known Synonyms Sweet herb sweet
leaf sugar leaf and honey leaf or simply
Stevia
(مثل عباد الشمس) العائله المركبه
(و زيادة فترات النهار تؤخر االزهار و تسيد من ترجمع االستيفاسىيد( ساعه نهار 13-11)من نباتات النهار القصير
bull The RootThe root of stevia plant is fibrous filamentary
and perennial abundantly branched and
strain distributed near the surface and is
the only organ of the plant that does not
contain stevioside Stem
Root
Foliage
جذور نبات االستيفيا ليفيه فتيلية والمعمرة متشعبه و موزعه بالقرب من سطح
التربه وهي الجهاز الوحيد من النبات الذي ال يحتوي علي مادة االستيفاسويد
المحليه
bull The stemThe stem is annual sub-woody more or less
pubescent with a tendency to lean is more
or less branched During early development
does not have branches becoming
multicaule after the first growing season
coming to produce up to 20 stems in 3 to 4
years Under optimal conditions the stem
can reach five feet in height
Stem
Root
Foliage
bull The leafThe leaves are oval or elliptic lanceolate
small simple toothed edge or margin
sometimes in whorls somewhat hairy
The leaf is the organ with the highest
content of sweetener
Stem
Root
Foliage
ورقه بسيطة بيضاويه صغيره مسننه
bull The FlowersStevia classified as a short-day plant
placing the critical photoperiod at
12 to 13 hours depending on the
ecotype
Flowers are hermaphrodite
small whitish in terminal or axillary short
chapters grouped in corymbose panicles
االزهار صغيره خنثي بيضاء في مجاميع
The hermaphrodite flower is small whitish in
terminal or axillary short chapters grouped in
corymbose panicles A plant takes more than
a month to produce all their flowers The
plant is self-incompatible sporophytic type
and classified as obligatory
apomictic Pollination is by insects
Compound name R1 R2
Stevioside β-Glc β-Glc-β-Glc(2rarr1)
RebaudiosideA β-Glc β-Glc-β-Glc
I
β-Glc (3rarr1)
Rebaudioside C β-Glcβ-Glc -α-Rha(2rarr1)
I
β-Glc (3rarr 1)
Dulcoside A β-Glc β-Glc - α -Rha(2rarrl)
Rubusoside β-Glc β-Glc
Steviolbioside H β-Glc - β-Glc (2rarr1)
RebaudiosideB H β-Glc - β-Glc (2rarr1)
I
β-Glc (3rarr1)
Steviol (RI = R2 = H) is the aglycone of the steviol glycosides Glc and Rha
represent respectively glucose and rhamnose sugar moieties
The seven specific steviol glycosides
The component glycosides
of particular interest for
stevia leaf sweetening
property are stevioside and
Reb A
Powdered Stevia extracts
with 85ndash97 percent Sweet
glycosides (200-300 times
sweeter than sugar
(Sucrose)
Always Keep In
Mind We are
cultivating Stevia
for its leaves
bull A variety of soil types ranging from course
textured sands to well drained loamsare suitable
for growing stevia
but not heavy clay or poorly drained sites
bull Stevia requires very good drainage system
bull Stevia can be grown on a wide range of soil with
pH range from 6 up to 9
bull soil should not be saline
bull Soils with very heavy clay content or high pH
should also be treated or avoided
Before planting each and every
soil sample should be properly
tested and analyzed by an expert
Soil type
bull Climate bull The plant is climatically suited to climates from
temperate to sub-tropical where temperatures
range between 21-43degC with an average of
24degC
bull During the growing season it
seems to thrive in a temperature
range of 15 degC to 34degC provided all
input resources and good
management practices are
incorporated
bull Plant Varieties
bull There are many of Stevia rebaudiana
varieties developed all around the world
These varieties have been developed
for different climate requirements and
Stevioside and rebaudioside content
bull The minimum market requirement of
Stevioside and rebaudioside is 9
bull The grower should select proper
varieties with adequate guarantees from
the planting material suppliers about
minimum assured stevioside and
rebaudioside contents
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
bull Stevia is an obligate short day plant with a critical day length of about 11-13 h
bull Long‐days delay flowering and increase stevasoid accumulation Extensive variability within populations for day length sensitivity has been reported
Stevia belongs to
Class Magnoliopsida (Dicotyledons)
Order Asterales
Family Asteraceae or Compositae
Stevia rebaudiana has many commonly
known Synonyms Sweet herb sweet
leaf sugar leaf and honey leaf or simply
Stevia
(مثل عباد الشمس) العائله المركبه
(و زيادة فترات النهار تؤخر االزهار و تسيد من ترجمع االستيفاسىيد( ساعه نهار 13-11)من نباتات النهار القصير
bull The RootThe root of stevia plant is fibrous filamentary
and perennial abundantly branched and
strain distributed near the surface and is
the only organ of the plant that does not
contain stevioside Stem
Root
Foliage
جذور نبات االستيفيا ليفيه فتيلية والمعمرة متشعبه و موزعه بالقرب من سطح
التربه وهي الجهاز الوحيد من النبات الذي ال يحتوي علي مادة االستيفاسويد
المحليه
bull The stemThe stem is annual sub-woody more or less
pubescent with a tendency to lean is more
or less branched During early development
does not have branches becoming
multicaule after the first growing season
coming to produce up to 20 stems in 3 to 4
years Under optimal conditions the stem
can reach five feet in height
Stem
Root
Foliage
bull The leafThe leaves are oval or elliptic lanceolate
small simple toothed edge or margin
sometimes in whorls somewhat hairy
The leaf is the organ with the highest
content of sweetener
Stem
Root
Foliage
ورقه بسيطة بيضاويه صغيره مسننه
bull The FlowersStevia classified as a short-day plant
placing the critical photoperiod at
12 to 13 hours depending on the
ecotype
Flowers are hermaphrodite
small whitish in terminal or axillary short
chapters grouped in corymbose panicles
االزهار صغيره خنثي بيضاء في مجاميع
The hermaphrodite flower is small whitish in
terminal or axillary short chapters grouped in
corymbose panicles A plant takes more than
a month to produce all their flowers The
plant is self-incompatible sporophytic type
and classified as obligatory
apomictic Pollination is by insects
Compound name R1 R2
Stevioside β-Glc β-Glc-β-Glc(2rarr1)
RebaudiosideA β-Glc β-Glc-β-Glc
I
β-Glc (3rarr1)
Rebaudioside C β-Glcβ-Glc -α-Rha(2rarr1)
I
β-Glc (3rarr 1)
Dulcoside A β-Glc β-Glc - α -Rha(2rarrl)
Rubusoside β-Glc β-Glc
Steviolbioside H β-Glc - β-Glc (2rarr1)
RebaudiosideB H β-Glc - β-Glc (2rarr1)
I
β-Glc (3rarr1)
Steviol (RI = R2 = H) is the aglycone of the steviol glycosides Glc and Rha
represent respectively glucose and rhamnose sugar moieties
The seven specific steviol glycosides
The component glycosides
of particular interest for
stevia leaf sweetening
property are stevioside and
Reb A
Powdered Stevia extracts
with 85ndash97 percent Sweet
glycosides (200-300 times
sweeter than sugar
(Sucrose)
Always Keep In
Mind We are
cultivating Stevia
for its leaves
bull A variety of soil types ranging from course
textured sands to well drained loamsare suitable
for growing stevia
but not heavy clay or poorly drained sites
bull Stevia requires very good drainage system
bull Stevia can be grown on a wide range of soil with
pH range from 6 up to 9
bull soil should not be saline
bull Soils with very heavy clay content or high pH
should also be treated or avoided
Before planting each and every
soil sample should be properly
tested and analyzed by an expert
Soil type
bull Climate bull The plant is climatically suited to climates from
temperate to sub-tropical where temperatures
range between 21-43degC with an average of
24degC
bull During the growing season it
seems to thrive in a temperature
range of 15 degC to 34degC provided all
input resources and good
management practices are
incorporated
bull Plant Varieties
bull There are many of Stevia rebaudiana
varieties developed all around the world
These varieties have been developed
for different climate requirements and
Stevioside and rebaudioside content
bull The minimum market requirement of
Stevioside and rebaudioside is 9
bull The grower should select proper
varieties with adequate guarantees from
the planting material suppliers about
minimum assured stevioside and
rebaudioside contents
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
bull The RootThe root of stevia plant is fibrous filamentary
and perennial abundantly branched and
strain distributed near the surface and is
the only organ of the plant that does not
contain stevioside Stem
Root
Foliage
جذور نبات االستيفيا ليفيه فتيلية والمعمرة متشعبه و موزعه بالقرب من سطح
التربه وهي الجهاز الوحيد من النبات الذي ال يحتوي علي مادة االستيفاسويد
المحليه
bull The stemThe stem is annual sub-woody more or less
pubescent with a tendency to lean is more
or less branched During early development
does not have branches becoming
multicaule after the first growing season
coming to produce up to 20 stems in 3 to 4
years Under optimal conditions the stem
can reach five feet in height
Stem
Root
Foliage
bull The leafThe leaves are oval or elliptic lanceolate
small simple toothed edge or margin
sometimes in whorls somewhat hairy
The leaf is the organ with the highest
content of sweetener
Stem
Root
Foliage
ورقه بسيطة بيضاويه صغيره مسننه
bull The FlowersStevia classified as a short-day plant
placing the critical photoperiod at
12 to 13 hours depending on the
ecotype
Flowers are hermaphrodite
small whitish in terminal or axillary short
chapters grouped in corymbose panicles
االزهار صغيره خنثي بيضاء في مجاميع
The hermaphrodite flower is small whitish in
terminal or axillary short chapters grouped in
corymbose panicles A plant takes more than
a month to produce all their flowers The
plant is self-incompatible sporophytic type
and classified as obligatory
apomictic Pollination is by insects
Compound name R1 R2
Stevioside β-Glc β-Glc-β-Glc(2rarr1)
RebaudiosideA β-Glc β-Glc-β-Glc
I
β-Glc (3rarr1)
Rebaudioside C β-Glcβ-Glc -α-Rha(2rarr1)
I
β-Glc (3rarr 1)
Dulcoside A β-Glc β-Glc - α -Rha(2rarrl)
Rubusoside β-Glc β-Glc
Steviolbioside H β-Glc - β-Glc (2rarr1)
RebaudiosideB H β-Glc - β-Glc (2rarr1)
I
β-Glc (3rarr1)
Steviol (RI = R2 = H) is the aglycone of the steviol glycosides Glc and Rha
represent respectively glucose and rhamnose sugar moieties
The seven specific steviol glycosides
The component glycosides
of particular interest for
stevia leaf sweetening
property are stevioside and
Reb A
Powdered Stevia extracts
with 85ndash97 percent Sweet
glycosides (200-300 times
sweeter than sugar
(Sucrose)
Always Keep In
Mind We are
cultivating Stevia
for its leaves
bull A variety of soil types ranging from course
textured sands to well drained loamsare suitable
for growing stevia
but not heavy clay or poorly drained sites
bull Stevia requires very good drainage system
bull Stevia can be grown on a wide range of soil with
pH range from 6 up to 9
bull soil should not be saline
bull Soils with very heavy clay content or high pH
should also be treated or avoided
Before planting each and every
soil sample should be properly
tested and analyzed by an expert
Soil type
bull Climate bull The plant is climatically suited to climates from
temperate to sub-tropical where temperatures
range between 21-43degC with an average of
24degC
bull During the growing season it
seems to thrive in a temperature
range of 15 degC to 34degC provided all
input resources and good
management practices are
incorporated
bull Plant Varieties
bull There are many of Stevia rebaudiana
varieties developed all around the world
These varieties have been developed
for different climate requirements and
Stevioside and rebaudioside content
bull The minimum market requirement of
Stevioside and rebaudioside is 9
bull The grower should select proper
varieties with adequate guarantees from
the planting material suppliers about
minimum assured stevioside and
rebaudioside contents
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
bull The stemThe stem is annual sub-woody more or less
pubescent with a tendency to lean is more
or less branched During early development
does not have branches becoming
multicaule after the first growing season
coming to produce up to 20 stems in 3 to 4
years Under optimal conditions the stem
can reach five feet in height
Stem
Root
Foliage
bull The leafThe leaves are oval or elliptic lanceolate
small simple toothed edge or margin
sometimes in whorls somewhat hairy
The leaf is the organ with the highest
content of sweetener
Stem
Root
Foliage
ورقه بسيطة بيضاويه صغيره مسننه
bull The FlowersStevia classified as a short-day plant
placing the critical photoperiod at
12 to 13 hours depending on the
ecotype
Flowers are hermaphrodite
small whitish in terminal or axillary short
chapters grouped in corymbose panicles
االزهار صغيره خنثي بيضاء في مجاميع
The hermaphrodite flower is small whitish in
terminal or axillary short chapters grouped in
corymbose panicles A plant takes more than
a month to produce all their flowers The
plant is self-incompatible sporophytic type
and classified as obligatory
apomictic Pollination is by insects
Compound name R1 R2
Stevioside β-Glc β-Glc-β-Glc(2rarr1)
RebaudiosideA β-Glc β-Glc-β-Glc
I
β-Glc (3rarr1)
Rebaudioside C β-Glcβ-Glc -α-Rha(2rarr1)
I
β-Glc (3rarr 1)
Dulcoside A β-Glc β-Glc - α -Rha(2rarrl)
Rubusoside β-Glc β-Glc
Steviolbioside H β-Glc - β-Glc (2rarr1)
RebaudiosideB H β-Glc - β-Glc (2rarr1)
I
β-Glc (3rarr1)
Steviol (RI = R2 = H) is the aglycone of the steviol glycosides Glc and Rha
represent respectively glucose and rhamnose sugar moieties
The seven specific steviol glycosides
The component glycosides
of particular interest for
stevia leaf sweetening
property are stevioside and
Reb A
Powdered Stevia extracts
with 85ndash97 percent Sweet
glycosides (200-300 times
sweeter than sugar
(Sucrose)
Always Keep In
Mind We are
cultivating Stevia
for its leaves
bull A variety of soil types ranging from course
textured sands to well drained loamsare suitable
for growing stevia
but not heavy clay or poorly drained sites
bull Stevia requires very good drainage system
bull Stevia can be grown on a wide range of soil with
pH range from 6 up to 9
bull soil should not be saline
bull Soils with very heavy clay content or high pH
should also be treated or avoided
Before planting each and every
soil sample should be properly
tested and analyzed by an expert
Soil type
bull Climate bull The plant is climatically suited to climates from
temperate to sub-tropical where temperatures
range between 21-43degC with an average of
24degC
bull During the growing season it
seems to thrive in a temperature
range of 15 degC to 34degC provided all
input resources and good
management practices are
incorporated
bull Plant Varieties
bull There are many of Stevia rebaudiana
varieties developed all around the world
These varieties have been developed
for different climate requirements and
Stevioside and rebaudioside content
bull The minimum market requirement of
Stevioside and rebaudioside is 9
bull The grower should select proper
varieties with adequate guarantees from
the planting material suppliers about
minimum assured stevioside and
rebaudioside contents
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
bull The leafThe leaves are oval or elliptic lanceolate
small simple toothed edge or margin
sometimes in whorls somewhat hairy
The leaf is the organ with the highest
content of sweetener
Stem
Root
Foliage
ورقه بسيطة بيضاويه صغيره مسننه
bull The FlowersStevia classified as a short-day plant
placing the critical photoperiod at
12 to 13 hours depending on the
ecotype
Flowers are hermaphrodite
small whitish in terminal or axillary short
chapters grouped in corymbose panicles
االزهار صغيره خنثي بيضاء في مجاميع
The hermaphrodite flower is small whitish in
terminal or axillary short chapters grouped in
corymbose panicles A plant takes more than
a month to produce all their flowers The
plant is self-incompatible sporophytic type
and classified as obligatory
apomictic Pollination is by insects
Compound name R1 R2
Stevioside β-Glc β-Glc-β-Glc(2rarr1)
RebaudiosideA β-Glc β-Glc-β-Glc
I
β-Glc (3rarr1)
Rebaudioside C β-Glcβ-Glc -α-Rha(2rarr1)
I
β-Glc (3rarr 1)
Dulcoside A β-Glc β-Glc - α -Rha(2rarrl)
Rubusoside β-Glc β-Glc
Steviolbioside H β-Glc - β-Glc (2rarr1)
RebaudiosideB H β-Glc - β-Glc (2rarr1)
I
β-Glc (3rarr1)
Steviol (RI = R2 = H) is the aglycone of the steviol glycosides Glc and Rha
represent respectively glucose and rhamnose sugar moieties
The seven specific steviol glycosides
The component glycosides
of particular interest for
stevia leaf sweetening
property are stevioside and
Reb A
Powdered Stevia extracts
with 85ndash97 percent Sweet
glycosides (200-300 times
sweeter than sugar
(Sucrose)
Always Keep In
Mind We are
cultivating Stevia
for its leaves
bull A variety of soil types ranging from course
textured sands to well drained loamsare suitable
for growing stevia
but not heavy clay or poorly drained sites
bull Stevia requires very good drainage system
bull Stevia can be grown on a wide range of soil with
pH range from 6 up to 9
bull soil should not be saline
bull Soils with very heavy clay content or high pH
should also be treated or avoided
Before planting each and every
soil sample should be properly
tested and analyzed by an expert
Soil type
bull Climate bull The plant is climatically suited to climates from
temperate to sub-tropical where temperatures
range between 21-43degC with an average of
24degC
bull During the growing season it
seems to thrive in a temperature
range of 15 degC to 34degC provided all
input resources and good
management practices are
incorporated
bull Plant Varieties
bull There are many of Stevia rebaudiana
varieties developed all around the world
These varieties have been developed
for different climate requirements and
Stevioside and rebaudioside content
bull The minimum market requirement of
Stevioside and rebaudioside is 9
bull The grower should select proper
varieties with adequate guarantees from
the planting material suppliers about
minimum assured stevioside and
rebaudioside contents
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
bull The FlowersStevia classified as a short-day plant
placing the critical photoperiod at
12 to 13 hours depending on the
ecotype
Flowers are hermaphrodite
small whitish in terminal or axillary short
chapters grouped in corymbose panicles
االزهار صغيره خنثي بيضاء في مجاميع
The hermaphrodite flower is small whitish in
terminal or axillary short chapters grouped in
corymbose panicles A plant takes more than
a month to produce all their flowers The
plant is self-incompatible sporophytic type
and classified as obligatory
apomictic Pollination is by insects
Compound name R1 R2
Stevioside β-Glc β-Glc-β-Glc(2rarr1)
RebaudiosideA β-Glc β-Glc-β-Glc
I
β-Glc (3rarr1)
Rebaudioside C β-Glcβ-Glc -α-Rha(2rarr1)
I
β-Glc (3rarr 1)
Dulcoside A β-Glc β-Glc - α -Rha(2rarrl)
Rubusoside β-Glc β-Glc
Steviolbioside H β-Glc - β-Glc (2rarr1)
RebaudiosideB H β-Glc - β-Glc (2rarr1)
I
β-Glc (3rarr1)
Steviol (RI = R2 = H) is the aglycone of the steviol glycosides Glc and Rha
represent respectively glucose and rhamnose sugar moieties
The seven specific steviol glycosides
The component glycosides
of particular interest for
stevia leaf sweetening
property are stevioside and
Reb A
Powdered Stevia extracts
with 85ndash97 percent Sweet
glycosides (200-300 times
sweeter than sugar
(Sucrose)
Always Keep In
Mind We are
cultivating Stevia
for its leaves
bull A variety of soil types ranging from course
textured sands to well drained loamsare suitable
for growing stevia
but not heavy clay or poorly drained sites
bull Stevia requires very good drainage system
bull Stevia can be grown on a wide range of soil with
pH range from 6 up to 9
bull soil should not be saline
bull Soils with very heavy clay content or high pH
should also be treated or avoided
Before planting each and every
soil sample should be properly
tested and analyzed by an expert
Soil type
bull Climate bull The plant is climatically suited to climates from
temperate to sub-tropical where temperatures
range between 21-43degC with an average of
24degC
bull During the growing season it
seems to thrive in a temperature
range of 15 degC to 34degC provided all
input resources and good
management practices are
incorporated
bull Plant Varieties
bull There are many of Stevia rebaudiana
varieties developed all around the world
These varieties have been developed
for different climate requirements and
Stevioside and rebaudioside content
bull The minimum market requirement of
Stevioside and rebaudioside is 9
bull The grower should select proper
varieties with adequate guarantees from
the planting material suppliers about
minimum assured stevioside and
rebaudioside contents
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
Compound name R1 R2
Stevioside β-Glc β-Glc-β-Glc(2rarr1)
RebaudiosideA β-Glc β-Glc-β-Glc
I
β-Glc (3rarr1)
Rebaudioside C β-Glcβ-Glc -α-Rha(2rarr1)
I
β-Glc (3rarr 1)
Dulcoside A β-Glc β-Glc - α -Rha(2rarrl)
Rubusoside β-Glc β-Glc
Steviolbioside H β-Glc - β-Glc (2rarr1)
RebaudiosideB H β-Glc - β-Glc (2rarr1)
I
β-Glc (3rarr1)
Steviol (RI = R2 = H) is the aglycone of the steviol glycosides Glc and Rha
represent respectively glucose and rhamnose sugar moieties
The seven specific steviol glycosides
The component glycosides
of particular interest for
stevia leaf sweetening
property are stevioside and
Reb A
Powdered Stevia extracts
with 85ndash97 percent Sweet
glycosides (200-300 times
sweeter than sugar
(Sucrose)
Always Keep In
Mind We are
cultivating Stevia
for its leaves
bull A variety of soil types ranging from course
textured sands to well drained loamsare suitable
for growing stevia
but not heavy clay or poorly drained sites
bull Stevia requires very good drainage system
bull Stevia can be grown on a wide range of soil with
pH range from 6 up to 9
bull soil should not be saline
bull Soils with very heavy clay content or high pH
should also be treated or avoided
Before planting each and every
soil sample should be properly
tested and analyzed by an expert
Soil type
bull Climate bull The plant is climatically suited to climates from
temperate to sub-tropical where temperatures
range between 21-43degC with an average of
24degC
bull During the growing season it
seems to thrive in a temperature
range of 15 degC to 34degC provided all
input resources and good
management practices are
incorporated
bull Plant Varieties
bull There are many of Stevia rebaudiana
varieties developed all around the world
These varieties have been developed
for different climate requirements and
Stevioside and rebaudioside content
bull The minimum market requirement of
Stevioside and rebaudioside is 9
bull The grower should select proper
varieties with adequate guarantees from
the planting material suppliers about
minimum assured stevioside and
rebaudioside contents
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
Always Keep In
Mind We are
cultivating Stevia
for its leaves
bull A variety of soil types ranging from course
textured sands to well drained loamsare suitable
for growing stevia
but not heavy clay or poorly drained sites
bull Stevia requires very good drainage system
bull Stevia can be grown on a wide range of soil with
pH range from 6 up to 9
bull soil should not be saline
bull Soils with very heavy clay content or high pH
should also be treated or avoided
Before planting each and every
soil sample should be properly
tested and analyzed by an expert
Soil type
bull Climate bull The plant is climatically suited to climates from
temperate to sub-tropical where temperatures
range between 21-43degC with an average of
24degC
bull During the growing season it
seems to thrive in a temperature
range of 15 degC to 34degC provided all
input resources and good
management practices are
incorporated
bull Plant Varieties
bull There are many of Stevia rebaudiana
varieties developed all around the world
These varieties have been developed
for different climate requirements and
Stevioside and rebaudioside content
bull The minimum market requirement of
Stevioside and rebaudioside is 9
bull The grower should select proper
varieties with adequate guarantees from
the planting material suppliers about
minimum assured stevioside and
rebaudioside contents
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
bull A variety of soil types ranging from course
textured sands to well drained loamsare suitable
for growing stevia
but not heavy clay or poorly drained sites
bull Stevia requires very good drainage system
bull Stevia can be grown on a wide range of soil with
pH range from 6 up to 9
bull soil should not be saline
bull Soils with very heavy clay content or high pH
should also be treated or avoided
Before planting each and every
soil sample should be properly
tested and analyzed by an expert
Soil type
bull Climate bull The plant is climatically suited to climates from
temperate to sub-tropical where temperatures
range between 21-43degC with an average of
24degC
bull During the growing season it
seems to thrive in a temperature
range of 15 degC to 34degC provided all
input resources and good
management practices are
incorporated
bull Plant Varieties
bull There are many of Stevia rebaudiana
varieties developed all around the world
These varieties have been developed
for different climate requirements and
Stevioside and rebaudioside content
bull The minimum market requirement of
Stevioside and rebaudioside is 9
bull The grower should select proper
varieties with adequate guarantees from
the planting material suppliers about
minimum assured stevioside and
rebaudioside contents
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
bull Climate bull The plant is climatically suited to climates from
temperate to sub-tropical where temperatures
range between 21-43degC with an average of
24degC
bull During the growing season it
seems to thrive in a temperature
range of 15 degC to 34degC provided all
input resources and good
management practices are
incorporated
bull Plant Varieties
bull There are many of Stevia rebaudiana
varieties developed all around the world
These varieties have been developed
for different climate requirements and
Stevioside and rebaudioside content
bull The minimum market requirement of
Stevioside and rebaudioside is 9
bull The grower should select proper
varieties with adequate guarantees from
the planting material suppliers about
minimum assured stevioside and
rebaudioside contents
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
bull Plant Varieties
bull There are many of Stevia rebaudiana
varieties developed all around the world
These varieties have been developed
for different climate requirements and
Stevioside and rebaudioside content
bull The minimum market requirement of
Stevioside and rebaudioside is 9
bull The grower should select proper
varieties with adequate guarantees from
the planting material suppliers about
minimum assured stevioside and
rebaudioside contents
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
bull Planting
bull The types of agronomic practices
generally depend on type of soil and
climate conditions
bull Since the economical part is the leaves
it is very important to achieve highest
vegetative growth
Generally it is advisable to plant
minimum of 30000 to 40000 plants per
feddan
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
bull Planting
Transplants from cuttings would be
superior when enough stocks of stem
cuttings are available Cuttings may
form roots without any treatment but it
is better to be treated with commercial
rooting hormones IBA before
cultivation
Stevia tip cuttings propagate in plug
trays placed in a greenhouse for a
period of 7 to 8 weeks before moving to
the field
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
bull Planting
bull A suitable alternative method to prepare
sufficient amount of plants within short
time duration is the use of In vitro
cultures The micropropagation of
plants through shoot tip or axillary bud
culture allows recovery of genetically
stable and true to type progeny Stevia
can form multiple shoots from nodal
explants which are convenient type of
culture for culturing on large scale
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
bull Appropriate planting time
Transplanting the plantlets is applied
and planting on Feb at the ideal
temperature degree as in this
month the absent plants are the
least than in other months
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
bull Land preparation
bull Land should be ploughed initially with a disc plough or harrowed
to break down the colds
bull Around 25 MT of farmyard
manure Feddan has to be
applied
bull Leaser leveling the field help
plants to grow and utilize
irrigation water
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
bull Raised bed preparation
bull Forming raised beds is the most economical way to grow Stevia The raised bed should be of 15 cm in height and 60 cm in width
The distance between each
plant 20 - 25cm This would
give a plant population of
around 30000 per feddan
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
bull Irrigation
bull Stevia requires ample supply of good water all year around As
the plant cannot tolerate drought It is recommended to have drip
irrigation or Micro sprinklers for better results Frequent irrigation
is required so through micro sprinklers the water can be
sprinklers the water can be sprinkled once in a day in winter and
two to four times in a day in summer depending upon the heat
and relative humidity in the air
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
bull Pinching tips
Since Stevia has a significant apical dominance
the plant tends to grow tall and lanky
Pinching out the tips of the plants about
every 3-4 weeks for first 1-2 months after
transplanting in the feild This will encourage
side branching
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
bull Fertilizer application
bull Balanced slow release organic fertilizer
or manure which is tilled into the soil
before planting provides the ideal
nutrition
bullThe recommended dose of fertilizer is
1104545 NPKfeddanyear
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
bull Weeding
Removal of weeds can be done manually Since the crop is grown
in raised beds intercultural operations are easier by manual
labour
bull Plastic mulch can be an effective means of controlling weeds if it
can cover more of the planted and interrow areas
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
bull Weeding
Herbicides such as Pendimethalin [N-(1-Ethylpropyl)-34-
dimethyl-26-dinitro-benzenamine34-Dimethyl-26-dinitro-N-(1
ethylpropyl)aniline] and Bromacil [5-bromo-6-methyl-3-(1-
methylpropyl)-24(1H3H)-pyrimidinedione] have been reported
high effective in controlling stevia annual grasses and certain
broadleaf weeds
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
bull Harvesting
bull Timing of harvest is an important aspect of harvesting It should
be noted that at no point of time plants should be allowed to
flower since after flowering the Stevioside percentage goes down
rapidly and leaves are rendered unmarketable
The first harvesting can be done four
to five months after planting (three
months after transplanting)
Subsequent harvesting can be done
every three months for three to five
consecutive years
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
Harvesting
bull Harvesting should be done as late as possible
bull Following harvest the whole plant is dried and the leaves
separated from the stems for further processing
bull If cut plant material is not dried quickly
leaf quality can deteriorate by oxidation
losing up to one third of stevioside
content after three days
A green dried leaf colour
is desirable and
represents good quality
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
Crushing
bull After drying leaves are stripped from the
stems by hand or a mechanical
thrasherseparator before leaves are packed
for transport to a processing facility
Crushing the dried leaves is the final step in
releasing Steviarsquos sweetening power The
dried leaves are powdered sieved and the
fine powder is stored in containers
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
Pests and Diseases
bull Diseases do not appear to be a major
problem to stevia although there are reports
claiming to record the first known incidence
of Sclerotinia leaf-spot Septoria black-spot
Alternaria Botrytis Fusarium (wilt)
Sclerotinia rolfsii (lsquowhite silkrsquo round the plant
stem) and Rhizoctonia (stem spots and
wilting of leaves)
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
Pests and Diseases
bull Stevia is usually the last plant insects will
feed on The plantrsquos sweetness seems to
provide a kind of natural defense against
aphids grasshoppers and other bugs Some
stevia varieties may be attacked by aphids
white fly and leaf cutters but not cause a
serious economic damage to the plants
White fly
الذبابه البيضاء
Mole cricket
Grasshoppersحشرة الحفار
Aphidsحشرة النطاط
حشرة المن
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
The use of dried leaves (pieces or powdered) is not
unacceptable in cooking and does leave a sediment in clear
drinks etc and can also leave a green colour in addition to
the unpleasant aroma associated with the dried leaves
Appropriate processing of the dry leaves can remove this
aroma which is due to specific leaf compounds (not
steviosides)
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
Methanol appears to be used in most extraction and
purification processes in Japan presumably to improve
extraction efficiency and facilitate the separation of individual
steviosides
More recent processing methods using water filtration
procedures do not use methanol and so produce a more
lsquonaturalrsquo product Newer factories in Brazil China and India use
water only extraction procedures and claim 96 purity of
product
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
Process Flow
Diagram for
stevia Extract
In brief steviol glycosides are obtained by the
extraction of stevia leaves with water Leaves from
different varieties of stevia plants are used for
stevioside and rebaudioside A production Ferric
chloride and calcium hydroxide are added to the extract
solution to facilitate precipitation The extraction
solution is passed through plate filtration followed by
adsorption onto resin the glycosides are subsequently
eluted with ethanol The solution is decolored with
active carbon and concentrated with film evaporators It
is again decolored with active carbon and filtered The
concentrate is spray dried to obtain the primary stevia
extracts rich in stevioside or rebaudioside A Meaning
Stevia Leaves
With STVge45 contents
Water ExtractionPurified Water
Precipitation
Filter
Ion Exchange
Decoloring with active carbon
Concentration
Sterile Filtration
Decoloring with active carbon
Spray drying
Packing
Quality control
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal
Process Flow
Diagram for
Purification
These extracts obtained from the first
extraction are further processed to obtain the
high purity stevioside and rebaudioside A
Dissolving materials
Crystalization
Filtration
Concentration
Sterile Filtration
Spray drying
Packing
Storage
Dissolving crystal