Taiwan- Japan Joint Meeting on Bioimaging for Young ...

Taiwan- Japan Joint Meeting on Bioimaging for Young Researchers November 1-2, 2017

Academia Sinica, Taipei, Taiwan

What is ？ A hot pot in which you don’t know what the ingredients are.

In this meeting, we anticipate emergence of a surprising flavor from the individual ingredients. Young

researchers from three groups in Taiwan and Japan bring cutting-edge imaging methods and applications,

aiming to establish connections/collaborations among participants in our current projects and also future

works.

Who should attend? Everybody interested in the field of imaging technologies and their applications in life sciences. The meeting

provides many opportunities for scientific presentations, discussions and social activities to promote interactions

among young researchers as well as senior investigators.

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Organizers Academia Sinica

Wei Yuan Yang

Chia-Lung Hsieh

Bi-Chang Chen Resonance Bio

Naoki Komatsu

Hiroko Sakurai 4D Cell

Kosuke DoDo

Kazuo Kurokawa

Yasuhiro Maeda

Satoko Takemoto

Masahiko Morita

Kaori Fukaya

Our Sponsors

November 1-2, 2017

Academia Sinica, Taipei, Taiwan

1-Nov

09:00-09:20 Opening Remarks & Introduction of Resonance Bio MIYAWAKI, Atsushi (RIKEN)Fluoppi: Visualization of protein protein interactions (PPIs)using liquid phase transitions

WATANABE, Taku (MBL Co.)

Development of a new murine model of bone metastasisusing bioluminescence imaging

KUCHIMARU, Takahiro (TokyoInstitute of Technology)

Tracking and Classification Method for Cells in the Images ofFluorescent Proteins

HASHIMOTO, Daiki (Mizuho Info.& Research Inc.)

Bio-image informatics for whole brain activity imaging TOYOSHIMA, Yu (Univ. of Tokyo)

Detection of LacZ-Positive Cells in Living Tissue with Single-Cell Resolution

KAMIYA, Mako (Univ. of Tokyo)

11:00-12:10 Lunch

12:10-12:30 Introduction of Academia Sinica Project LIAO, Jung-Chi (IAMS)

Beyond the diffraction limit by light -shieet microscopy CHEN, Bi-Chang (RCAS)

Label-free interferometric imaging resolves high-speeddynamics of single bio-nanoparticles in live cells

HSIEH, Chia-Lung (IAMS)

From Seeing to Believing: Visualization and Tracking ofExtracellular Vesicles

LAI, Charles Pin-Kuang (IAMS)

Upconversion Nanoparticle as Light Emitting PhotoactivationPlatform

LEE, Hsien-Ming (IoC)

Integrated microfluidic platform for cell signaling study TU, Hsiung-Lin (IoC)

Light-induced Neurotoxic Aggregates in NeurodegenerativeDisease

HUANG, Jen-Tse (IoC)

14:30-14:45 Break

14:45-17:15 Poster Flash Talk

17:15-17:20 Group photo

17:20-19:00 Poster Session

19:00- Banquet

Banquet

12:30-14:30

Taiwan-Japan Joint Meeting on Bioimaging for Young Researchers-Academia Sinica - 4D Cell - ResonanceBio -

Session 1: Resonance Bio (Conference Room 1, Level 3)

Session 2: Academia Sinica (Conference Room 1, Level 3)

09:20-11:00

Poster Session (South & North Wing, Level 4)

2-Nov

09:00-09:20 Introduction of 4D Cell Project NAKANO, Akihiko (RIKEN RAP)

Development of Laser for 4D Cell Imaging MAEDA, Yasuhiro (RIKEN RAP)

Modulation microscopy for super-resolution deep imaging ISOBE, Keisuke (RIKEN RAP)

Single molecule measurement of core transcriptional factorsin living nuclei of mouse embryonic stem cells

OKAMOTO, Kazuko (RIKEN QBiC)

Intercellular propagation of EGF-ERK signaling by relaymechanism directs epithelial invagination

OGURA, Yousuke (RIKEN CDB)

Physical modeling of Golgi formation process TACHIKAWA, Masashi (RIKEN ILs)

11:10-12:30 Round Table Discussion

12:30-14:00 Lunch

SOGA, Kohei (RB: Tokyo Univ of Science)NEMOTO, Tomomi (RB: Hokkaido Univ)HOTTA, Kazuhiro (RB: Meijo Univ)CHANG, Huan-Cheng (AS: IAMS)CHEN, Pei-Lin (AS: RCAS)CHOU, Chia-Fu (AS: IoP)NAKANO, Akihiko (4D: RIKEN RAP) ONAMI, Shuichi (4D: RIKEN QBiC)OKADA, Yasushi (4D: RIKEN QBiC)

Poster Award

Closing Remarks

16:00-18:00 Lab Visit

Session 3: 4D Cell (Conference Room 1, Level 3)

Round Table Discussion

Panel Discussion & Award Celemony (Conference Room 1, Level 3)

Lab Visit (Applicants only)

15:00-15:30

14:00-15:00 Panel Discussion

09:20-11:00

Poster Session

Poster Session

Lunch & Banquet

Conference room

Level 3

Level 4

3

OralNo. Author Affiliation Titile

1 MIYAWAKI Atsushi宮脇敦史 RIKEN Brain Science Institute Introduction of Resonance Bio

2 WATANABE Taku渡部拓

R&D DivisionMedical & Biological Laboratories Co Ltd

Fluoppi: Visualization of protein protein interactions (PPIs)using liquid phase transitions

3 KUCHIMARU Takahiro口丸高弘

Kondoh LaboratoryTokyo Institute of Technology

Development of a new murine model of bone metastasis usingbioluminescence imaging

4 HASHIMOTO Daiki橋本大樹

Information and Communication Research DivisionMizuho Information & Research Institute, Inc.

Tracking and Classification Method for Cells in the Images ofFluorescent Proteins.

5 TOYOSHIMA Yu豊島有

Lab. of Molecular genetics and ethologyUniversity of Tokyo Bio-image informatics for whole brain activity imaging

6 KAMIYA Mako神谷真子

Lab. of Chemical Biology and Molecular ImagingThe University of Tokyo


PosterNo. Author Affiliation Title

RB-01 WATANABE Taku渡部拓

R&D DivisionMedical & Biological Laboratories Co Ltd Applications of Fluoppi method visualizing PPIs in live cells

RB-02 TOYOSHIMA Yu豊島有

Lab. of Molecular genetics and ethologyUniversity of Tokyo Bio-image informatics for whole brain activity imaging

RB-03 HIRAMATSU Yuki平松侑樹

Hotta LaboratoryMeijo University

Object Segmentation by Integrating Multiple DeepNetworks

RB-04 HOSOKAWA Tomohisa細川智永

Lab. for Cell Function DynamicsRIKEN Brain Science Institute

Analysis for the activity and phosphorylation states ofsynaptic proteins.

RB-05 IMANISHI Ayako今西彩子

Laboratory of Bioimaging and Cell SignalingKyoto University

A novel method of analyzing molecular activities at single celllevel

RB-06 UMEZAWA Masakazu梅澤雅和

Soga LaboratoryTokyo University of Science

Challenge to 3-Dimentional Imaging of Brain Vasculature byNear-Infrared Fluorescence Probe with Light-Sheet ExcitationSystem

RB-07 ISHII Hirokazu石井宏和

Research Institute for Electronic ScienceHokkaido University

Treadmilling of microtubules drives the directional transport ofmaternal factors in fertilized ascidian eggs

RB-08 KUCHIMARU Takahiro口丸高弘

Kondoh LaboratoryTokyo Institute of Technology

Development of a new murine model of bone metastasis usingbioluminescence imaging

RB-09 OKABE Kohki岡部　弘基

Graduate School of Pharmaceutical SciencesUniversity of Tokyo Imaging and Manipulation of Temperature in Single Living Cells

RB-10 HASHIMOTO Daiki橋本大樹

Information and Communication Research DivisionMizuho Information & Research Institute, Inc.

Detection and Tracking Method for Cells Using ImageProcessing.

RB-11 GAO Jingchi高靖馳

Laboratory of Chemical BiologyOsaka University A fluorescent probe for quick live-cell protein labeling

RB-12 YEROSLAVSKY Gil Laboratory for Bio-imagingTokyo University of science

Visual Mapping of Strain in Elastic Silicone Polymers Based onEnergy Transfer Phenomena

RB-13 KOMATSU Naoki小松直貴

Lab. for Cell Function DynamicsRIKEN Brain Science Institute

Development of fluorescent/luminescent protein-basedbiosensors for visualizing kinase activity in living cells

RB-14 MINEGISHI Misa峯岸美紗

Kondoh laboratoryTokyo Institute of Technology

A multi-bioluminescence reporter system visualizes crosstalkbetween cancer cells and bone marrow microenvironment

RB-15 MOROZUMI Akihico両角明彦

Lab. of Chemistry and BiologyThe University of Tokyo

Development of spontaneously blinking fluorophores based onnucleophilic addition of intracellular glutathione for super-resolution imaging

RB-16 YAMAGUCHI Kazushi山口和志


Two--photon imaging within living mouse brains improved byeasy modifications in the laser irradiation condition

RB-17 KAMIMURA Masao上村真生

Soga LaboratoryTokyo University of Science

Over-1000 nm Near-Infrared (OTN-NIR) Fluorescence in vivoImaging

RB-18 OTOMO Kohei大友康平


Multi-point scanning two-photon microscopy by utilizing a novelneodymium based laser

RB-19 KAMIYA Mako神谷真子

Lab. of Chemical Biology and Molecular ImagingThe University of Tokyo


RESONANCE BIO

OralNo. Author Affiliation Titile

1 LIAO, Jung-Chi廖仲麒

Institute of Atomic and Molecular SciencesAcademia Sinica Introduction of Academia Sinica

2 CHEN, Bi-Chang陳壁彰

Research Center for Applied SciencesAcademia Sinica Beyond the diffraction limit by light -shieet microscopy

3 HSIEH, Chia-Lung謝佳龍

Institute of Atomic and Molecular SciencesAcademia Sinica

Label-free interferometric imaging resolves high-speed dynamicsof single bio-nanoparticles in live cells

4 LAI, Charles Pin-Kuang賴品光


From Seeing to Believing: Visualization and Tracking ofExtracellular Vesicles

5 LEE, Hsien-Ming李賢明

Institute of ChemistryAcademia Sinica


6 TU, Hsiung-Lin涂熊林

Institute of ChemistryAcademia Sinica Integrated microfluidic platform for cell signaling study

7 HUANG, Jen-Tse黃人則




AS-01 HUANG, Cherry Yu-Shan黃鈺珊

Institute of PhysicsAcademia Sinica

3D Time-Lapsed in vivo Imaging Study of Eye Development inDrosophila Embryo via Dual-illumination Light SheetMicroscopy

AS-02 CHU, Li-An朱麗安

Institute of PhysicsAcademia Sinica Whole brain 5D functional imaging

AS-03 LIN, Keng-Hui林耿慧

Institute of PhysicsAcademia Sinica

The Origins of Apical Constriction Force of Madin--DarbyCanine Kidney (MDCK) Cysts

AS-04 WANG, Po-Hsiang王柏翔

Institute of PhysicsAcademia Sinica Topological influence on cell mechanics and signaling

AS-05 WU, Jheng-Syong吳正雄


A FRET Probe Strategy Based on Affinity Tag and Alternating-Laser Excitation Technique to Study the Position of the GeneralTranscription Factor TFIIF in the RNA Polymerase IITranscription Preinitiation Complex

AS-06 HUANG, Jen-Tse黃人則



AS-07 TU, Hsiung-Lin涂熊林

Institute of ChemistryAcademia Sinica Integrated Platform for Cell Signaling Study

AS-08 LEE, Hsien-Ming李賢明



AS-09 GAO, Hua-De高華德


NIR Photoactivation of Protein Kinase A Using UpconversionNanoparticles

AS-10 HSIEH, Feng Jen謝豐任


Lipid-Encapsulated Fluorescent Nanodiamonds for CorrelativeLight Electron Microscopy of Cell Surface Antigens

AS-11 TSENG, Ting-Yuan曾鼎元


Bioimaging of fluorescent probes reveals a potential universalcancer biomarker: G-quadruplex foci

AS-12 LAI, Charles Pin-Kuang賴品光


Subcellular and Whole Animal Imaging of Extracellular Vesicleswith Engineered Fluorescent and Bioluminescent Reporters

AS-13 WU, Anthony Yan-Tang吳晏瑭


A Method-Comparison Study of Extracellular Vesicle Labelingfor in vitro and in vivo Imaging

AS-14 CHONG, Weng Man鍾穎文


Superresolution Imaging Reveals Staggered Architecture of theDistal

AS-15 YANG, Tony楊東霖


Live--cell superresolution tracing reveals zone--dependentnonaxonomal dynamics of intraflagellar transport proteins at theciliary base

AS-16 LI, Li-Tzu李莉姿


Superresolution Imaging Reveals Redirection of IntraflagellarTransport Proteins in Transition Zone--Deficient MammalianCells

AS-17 HSIEH, Chia-Lung謝佳龍


Label-free interferometric imaging resolves high-speed dynamicsof single bio-nanoparticles in live cells

AS-18 LIAO, Yi-Hung廖宜鴻


Nanoscale single-molecule dynamics in cell membranes byultrahigh-speed microscopy

Academia Sinica

Poster

No. Author Affiliation Title

AS-19 TSAI, YUN-CHI蔡允齊

Research Center for Applied ScienceAcademia Sinica Light sheet fluorescence imaging on expanded samples

AS-20 LU, Chieh-Han呂杰翰

Research Center for Applied ScienceAcademia Sinica

Rapid, Large-scale, Three-Dimensional Super-ResolutionMicroscopy

AS-21 LIU, Yen-Ting劉彥廷


Instrument Control, Numerical Evaluation and High-throughputData Processing

AS-22 CHEN, Chin-Yi陳靜怡


To monitor the subcellular voltage & Ca2+ dynamics ofhippocampal neurons on matrigel-based 3D culture by light-sheetmicroscopy

AS-23 TANG, Wei-Chun湯為淳


Developing fluorescent probes for cytoskeleton of migratingcells and monitoring them by lattice light-sheet microscopy

AS-24 LEE, Chia-Wei李家瑋


Looking into tunneling nanotubes between pancreatic cancercells induced by the macrophage conditioned medium andelectric fields

AS-25 KUO, Chiung Wen郭瓊雯


Real-Time Intravital Imaging for Revealing the Rare Cells in theBlood Vessels

AS-26 Chen, Chen-Hui陳振輝

Institute of Cellular and Organismic BiologyAcademia Sinica Live imaging of tissue regeneration in zebrafish

AS-27 HUANG, Wan-Chen黃婉媜

Institute of Cellular and Organismic BiologyAcademia Sinica

Dynamic analysis of DNA-topoisomerase II interaction based onfluorescence fluctuation and single molecule detection

AS-28 FU, Chiyu傅琪鈺


Electron tomographic analysis reveals ultrastructural features ofmitochondrial cristae architecture which reflect energetic stateand aging

AS-29 LEE, Yi-Ching李宜靜


Regulation of intracellular trafficking of a multipletransmembrane protein, GLUT10

AS-30 CHEN, Chun Wei陳君瑋

Institute of Biological ChemistryAcademia Sinica

An image-based screening strategy for deubiquitinases inselective autophagy

AS-31 SANG, Chun桑淳

Institute of BiochemistryAcademia Sinica

ER to mitochondrial calcium transfer drives autophagy burstsduring starvation

AS-32 CHEN, Bo-Hua陳柏樺

Institute of BiochemistryAcademia Sinica Hsp70 quality controls peroxisomes

AS-33 CHEN, Sheng-hong陳昇宏

Institute of Molecular BiologyAcademia Sinica Functional Dynamics of Cellular Adaptation in Single Cells

AS-34 CHEN, Hung-Ta陳宏達

Institute of Molecular BiologyAcademia Sinica

Biochemical and biophysical analyses of the eukaryotic RNApolymerase III transcription cycle

AS-35 WANG, Rachel Chung-Ju王中茹

Institute of Plant and Microbial BiologyAcademia Sinica

Maize Synaptonemal Complex Structure Revealed by Super--Resolution Microscopy

AS-36 LEE, Ding-Hua李頂華

Institute of Plant and Microbial BiologyAcademia Sinica

Patterns of SUMOylation and Ubiquitylation in Maize MeiosisAnalyzed by Super-Resolution Microscopy

AS-37 LIU, Pei-Jou劉珮柔

National Taiwan University Institute of BiochemicalSciences

Identification of Autophagic Clearance for Damaged PlasmaMembrane

OralNo. Author Affiliation Title

1NAKANO Akihiko中野中野明彦

Live Cell Super-Resolution Imaging Reseach Team,RIKEN Center for Advanced Photonics

Introduction of 4D Cell Project

2MAEDA Yasuhiro前田前田康大

Photonics Control Technology Team,RIKEN Center for Advanced Photonics

Development of Laser for 4D Cell Imaging

3ISOBE Keisuke磯部磯部圭佑

Attosecond Science Research Team,RIKEN Center for Advanced Photonics

Modulation microscopy for super-resolution deep imaging

4OKAMOTO Kazuko岡本岡本和子

Laboratory for Comprehensive Bioimaging,RIKEN Quantitative Biology Center

Single molecule measurement of core transcriptional factors in living nuclei of mouseembryonic stem cells

5OGURA Yosuke小椋小椋陽介

Laboratory for Morphogenetic Signaling,RIKEN Center for Developmental Biology

Intercellular propagation of EGF-ERK signaling by relay mechanism directs epithelialinvagination

6TACHIKAWA Masashi立川立川正志

Theoretical Biology Laboratory, RIKEN Physical modeling of Golgi formation process


4D-01ISOBE Keisuke磯部磯部圭佑

Attosecond Science Research Team,RIKEN Center for Advanced Photonics

Deep imaging techniques using spatio-temporal modulation

4D-02MAEDA Yasuhiro前田前田康大

Photonics Control Technology TeamRIKEN Center for Advanced Photonics

Development of Laser for 4D Cell Imaging

4D-03DODO Kosuke闐闐闐闐孝介

Synthetic Organic Chemistry Laboratory, RIKEN Alkyne-tag Raman Imaging for Visualization of Small Molecules

4D-04EGOSHI Syusuke江越江越脩祐

Synthetic Organic Chemistry Laboratory, RIKENProposal of new mechanism with in vivo Raman Imaging:coronatine induces the infection of plant pathogens independently of known receptors

4D-05KUROKAWA Kazuo黒川黒川量雄

Live Cell Super-Resolution Imaging Reseach TeamRIKEN Center for Advanced Photonics

4D imaging of cargo delivery in maturating Golgi cisternae

4D-06Tojima Takuro戸島戸島拓郎


4D imaging of coat and adaptor proteins on the trans -Golgi network

4D-07ITO Yoko伊藤伊藤容子


COPII-independent core of the Golgi apparatus functions as the scaffold for Golgiregeneration in plant cells

4D-08MIYASHIRO Daisuke宮代宮代大輔


Observations of the membrane traffic in living yeast cells via the high-speed super-resolution optical microscope

4D-09TACHIKAWA Masashi立川立川正志

Theoretical Biology Laboratory, RIKEN Physical modeling of Golgi formation process

4D-10TAKEMOTO Satoko竹本竹本智子

Image Processing Research TeamRIKEN Center for Advanced Photonics

Sommelier: A system for performance evaluation of image segmentation methodstoward better objectivity ofcell image analysis

4D-11MORITA Masahiko森田森田正彦

Image Processing Research TeamRIKEN Center for Advanced Photonics

Cloud computing system for image processing and sharing in 4D Cell project

4D-12TSAI Ming-Dar蔡蔡明達

Image Processing Research TeamRIKEN Center for Advanced PhotonicsInformation and Computer EngineeringChung-Yuan Christian University

Human Induced Pluripotent Stem Cell Region Recognition in Microscopy ImagesUsing Convolutional Neural Networks

4D-13AZUMA Yusuke東東裕介

Laboratory for Developmental DynamicsRIKEN Quantitative Biology Center

Quantitative analysis of variability of cellular dynamics in C. elegans embryogenesis

4D-14KYODA Koji京田京田耕司


Quantitative cell division dynamics data of RNAi-treated embryos for all essentialembryonic genes in C. elegans

4D-15SHINKAI Soya新海新海創也


Bridging the gap between the dynamics and organization of chromatin domains bymathematical modeling

4D-16 ATUPELAGE ChamiduLaboratory for Developmental DynamicsRIKEN Quantitative Biology Center

Deep neural network based segmentation method for detecting the nuclei in 4D DICmicroscopic images of C. elegans embryos

4D-17OKAMOTO Kazuko岡本岡本和子

Laboratory for Comprehensive BioimagingRIKEN Quantitative Biology Center

Single molecule measurement of core transcriptional factors in living nuclei of mouseembryonic stem cells

4D-18ICHIMURA Taro市村市村垂生

Laboratory for Comprehensive BioimagingRIKEN Quantitative Biology Center

Light scattering spectroscopy and imaging for cellular state quantification

4D-19WEN Fu-Lai溫溫福來

Laboratory for Physical BiologyRIKEN Quantitative Biology Center

Cell rearrangements in Drosophila tracheal tube elongation

4D-20CHU Wei-Chen朱朱韋臣

Laboratory for Morphogenetic SignalingRIKEN Center for Developmental Biology

The role of apical extracellular matrix in force transmission and epithelialmorphogenesis

4D-21OGURA Yosuke小椋小椋陽介

Laboratory for Morphogenetic SignalingRIKEN Center for Developmental Biology

Intercellular propagation of EGF-ERK signaling by relay mechanism directs epithelialinvagination

4D-22MASE Shun間瀬間瀬俊

Laboratory for Cell AsymmetryRIKEN Center for Developmental Biology

Notch Signaling Dynamics for the Asymmetric Cell Fate Determination in the MouseNeurogenesis

4D-23SHIOI Go塩井塩井剛

Genetic Engineering TeamRIKEN Center for Life Science Technologies

Collective cell rearrangement in visceral endoderm during the anterior-posterior axisformation in a pregastrula mouse embryo

4D Cell

Resonance Bio Name Affiliation

PanelsitSOGA Kohei曽我曽我公平

Tokyo University of Science

PanelsitNEMOTO Tomomi根本　知己

Research Institute for Science, Hokkaido University

PanelistHOTTA Kazuhiro堀田堀田一弘

Meijo University

Attendance OnlyIMAMURA Takeshi今村今村健志

Ehime University Graduate School of Medicine

AdministratorSAKURAI Hiroko櫻井櫻井紘子

RIKEN Brain Scinece InstituteRIKEN Center for Advanced Photonics

Academia Sinica Name Affiliation

OrganizerYANG Wei-Yuan楊維元

Institute of Biological Chemistry, Academia Sinica

PanelistChen, Peilin陳培菱

Research Center for Applied Sciences, Academia Sinica

Attendance OnlyChen, Yen Wei陳彥瑋

Institute of Atomic and Molecular Sciences, Academia Sinica

Attendance OnlyChen, Zhengmin陳正民


Attendance OnlySOTOMA Shingo外間外間進悟


Attendance OnlyHSUEH Ching-chong薛景中

Institute of Physics, Academia Sinica

Attendance OnlyCheng, Ching-Ya鄭敬亞


Attendance OnlyLin, Chih-Hsiang林智翔


Attendance OnlyChristine Wong黃慧清


4D Cell Name Affiliation

PanelistOKADA Yasushi岡田康志

Laboratory for Cell Polarity Regulation, RIKEN QBiC

PanelistONAMI Shuichi大浪修一

Laboratory for Developmental Dynamics, RIKEN QBiC

PanelistHAYASHI Shigeo林茂生

Laboratory for Morphogenetic Signaling, RIKEN CDB

AdministratorFUKAYA Kaori深谷深谷香織

Live Cell Super-Resolution Imaging Reseach Team, RIKEN Center for Advanced Photonics

Other Participants

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