SOR Advanced Bioimaging Brochure
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CORE PLATFORM CORE PLATFORM
description
Overview brochure of SingHealth Office of Research core platform, Advanced Bioimaging.
Transcript of SOR Advanced Bioimaging Brochure
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BACKGROUND
SingHealth Advanced Bioimaging aims to establish a bioimaging hub for investigators in SingHealth and Singapore. This is by providing a wide variety of imaging platforms and services in both light and electron microscopy.
KEY OBJECTIVES
Provide guidance and expertise in image acquisition and analysis to investigators
Provide imaging services to investigators in SingHealth and Singapore
Educate and train investigators in bioimaging techniques through conducting workshops, seminars, and hands-on training sessions
CORE STRENGTHS & SERVICES
SingHealth Advanced Bioimaging provides imaging services for both light and electron microscopy.We focus on super-resolution light microscopy, including:
The table below summarises the lateral (XY) and axial (Z) resolutions that can be obtained with each technique:
Imaging Technique Lateral Resolution (XY) Axial Resolution (Z)
SIM 120 nm 250 nm
STED-3X 50-70 nm 70-130 nm
3D-STORM 10-30 nm 50-70 nm
3D-PALM 10-30 nm 50nm
Structured Illumination Microscopy (SIM)
Stimulated Emission Depletion microscopy (STED)
3D-Stochastic Optical Reconstruction Microscopy (3D-STORM)
3D-Photoactivated Localisation Microscopy (3D-PALM)
For image processing, we provide two HP Z420 workstations installed with the following scienti�c image rendering and analysis software: NIS-Elements, ImageJ, Imaris, Huygens, LAS-AF Lite, MetaXpress, ZEN, and ZEN Lite.
For electron microscopy, we provide ultra-thin tissue sample preparation and imaging services for Transmission Electron Microscopy (TEM). SingHealth Advanced Bioimaging owns a Joel JEM 2100 TEM and state-of-the-art EM preparation equipment from Leica. The EM preparation room is supplied with necessary chemicals and reagents for ultra-thin tissue sectioning, staining, coating and treatment. Below is a list of our equipment:
Leica EM KMR3 glass knife maker
Leica EM ACE200 sputter coater
Leica EM CPD300 critical point dryer
We have a fully functioning cell culture room, equipped with a CO2 incubator, a phase contrast microscope and a laminar �ow hood for live cell/tissue treatment.
The following list of platforms can be booked via our Google calendar:
Nikon N-STORM (BF, WF, TIRF, live cell, d-STORM & N-STORM)
Leica STED-3X (3D-STED and confocal)
ZEISS Elyra PS.1 (WF, TIRF, SIM, PALM & d-STORM)
Molecular Devices MetaXpress (HCS & live cell)
Image processing WS1 (NIS-Elements, Imaris, MetaXpres, ImageJ, ZEN lite, LAS-AF lite)
Image processing WS2 (NIS-Elements, ZEN, Huygens, ImageJ, ZEN lite, LAS-AF lite)
EM sample preparation lab (glass knife maker, sputter coater, critical point dryer and staining machine)
Leica ULTRACUT EM UC7 ultra microtome
Joel JEM 2100 TEM
Leica LMD 7000
SingHealth Advanced Bioimaging also provides access to services for the following conventional imaging techniques in light microscopy:
BF
DIC
WF
TIRF
BF & WF
LSCM
HCS
LMD
SD
Bright Field
Di�erential Interference Contrast
Wide-Field Fluorescence
Total Internal Re�ection Fluorescence
Live-Cell Imaging
Laser Scanning ConfocalMicroscopy
High-Content Screening
Laser Microdissection
Spinning Disk Confocal
Leica EM AC20 staining machine
Leica ULTRACUT EM UC7 ultra microtome
Joel JEM 2100 TEM
The content is correct at the time of printing.©2015 SingHealth O�ce of Research. All rights reserved.
DIRECTOR’S BIO
Assoc. Prof Antonius Van Dongen is Director of SingHealth Advanced Bioimaging. He is also Associate Professor at Duke-NUS Graduate Medical School Singapore. His current projects investigate epigenetic mechanisms of memory consolidation and memory processes in generic cortical microcircuits.
To make a booking for our facilities involves a simple 3-step process:
Training, imaging services and hands-on help are available upon request, for a fee.
For collaboration opportunities, please contact:
SingHealth Advanced Bioimaging Director: Assoc. Prof Antonius Van Dongen via [email protected] orSingHealth Advanced Bioimaging Manager: Dr. James Deqiang Zhao via [email protected]
-SingHealth Advanced Bioimaging
20 College Road,The Academia, Discovery Tower, Level 10Singapore 169856
Tel: (+65) 6576 7186Email: [email protected]
ENQUIRYFor imaging and payment enquiries, email to [email protected]
IMAGINGBook equipment and conduct imaging independently; or book a timeslot with our sta�if hands-on help is needed
PAYMENTCoordinate with sta� to complete paper work for usage charges
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