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![Page 1: Systematic identification of 3′-UTR regulatory elements in activity-dependent mRNA stability in hippocampal neurons by Jonathan E. Cohen, Philip R. Lee,](https://reader036.fdocuments.net/reader036/viewer/2022062804/56649ee15503460f94bf265c/html5/thumbnails/1.jpg)
Systematic identification of 3′-UTR regulatory elements in activity-dependent mRNA stability in hippocampal neurons
by Jonathan E. Cohen, Philip R. Lee, and R. Douglas Fields
Philosophical Transactions BVolume 369(1652):20130509
September 26, 2014
©2014 by The Royal Society
![Page 2: Systematic identification of 3′-UTR regulatory elements in activity-dependent mRNA stability in hippocampal neurons by Jonathan E. Cohen, Philip R. Lee,](https://reader036.fdocuments.net/reader036/viewer/2022062804/56649ee15503460f94bf265c/html5/thumbnails/2.jpg)
Activity-dependent, post-transcriptional gene regulation experiment.
Jonathan E. Cohen et al. Phil. Trans. R. Soc. B 2014;369:20130509
©2014 by The Royal Society
![Page 3: Systematic identification of 3′-UTR regulatory elements in activity-dependent mRNA stability in hippocampal neurons by Jonathan E. Cohen, Philip R. Lee,](https://reader036.fdocuments.net/reader036/viewer/2022062804/56649ee15503460f94bf265c/html5/thumbnails/3.jpg)
(Opposite.) Rapid, post-transcriptional regulation in hippocampal neurons mirrors activity-regulated genes in DRG axons.
Jonathan E. Cohen et al. Phil. Trans. R. Soc. B 2014;369:20130509
©2014 by The Royal Society
![Page 4: Systematic identification of 3′-UTR regulatory elements in activity-dependent mRNA stability in hippocampal neurons by Jonathan E. Cohen, Philip R. Lee,](https://reader036.fdocuments.net/reader036/viewer/2022062804/56649ee15503460f94bf265c/html5/thumbnails/4.jpg)
Discovery of enriched motifs for destabilized and stabilized genes. 3′-UTR analysis by MEME on downregulated (destabilized) and upregulated (stabilized) transcripts by MEME identifies miRNA-
binding sites.
Jonathan E. Cohen et al. Phil. Trans. R. Soc. B 2014;369:20130509
©2014 by The Royal Society
![Page 5: Systematic identification of 3′-UTR regulatory elements in activity-dependent mRNA stability in hippocampal neurons by Jonathan E. Cohen, Philip R. Lee,](https://reader036.fdocuments.net/reader036/viewer/2022062804/56649ee15503460f94bf265c/html5/thumbnails/5.jpg)
Predicted miRNAs are developmentally regulated in the hippocampus and may control nervous system development.
Jonathan E. Cohen et al. Phil. Trans. R. Soc. B 2014;369:20130509
©2014 by The Royal Society
![Page 6: Systematic identification of 3′-UTR regulatory elements in activity-dependent mRNA stability in hippocampal neurons by Jonathan E. Cohen, Philip R. Lee,](https://reader036.fdocuments.net/reader036/viewer/2022062804/56649ee15503460f94bf265c/html5/thumbnails/6.jpg)
miR-485 blocks NGF-induced neurite outgrowth in PC12 cells. miR-485 overexpression inhibits NGF-induced neurite outgrowth in PC12 cells.
Jonathan E. Cohen et al. Phil. Trans. R. Soc. B 2014;369:20130509
©2014 by The Royal Society
![Page 7: Systematic identification of 3′-UTR regulatory elements in activity-dependent mRNA stability in hippocampal neurons by Jonathan E. Cohen, Philip R. Lee,](https://reader036.fdocuments.net/reader036/viewer/2022062804/56649ee15503460f94bf265c/html5/thumbnails/7.jpg)
miR-485 decreases tau expression in hippocampal neurons.
Jonathan E. Cohen et al. Phil. Trans. R. Soc. B 2014;369:20130509
©2014 by The Royal Society
![Page 8: Systematic identification of 3′-UTR regulatory elements in activity-dependent mRNA stability in hippocampal neurons by Jonathan E. Cohen, Philip R. Lee,](https://reader036.fdocuments.net/reader036/viewer/2022062804/56649ee15503460f94bf265c/html5/thumbnails/8.jpg)
miR-485 overexpression controls tau expression and axonal outgrowth in hippocampal neurons. miR-485-5p overexpression reduced the extent of axonal outgrowth in hippocampal neurons.
Jonathan E. Cohen et al. Phil. Trans. R. Soc. B 2014;369:20130509
©2014 by The Royal Society