BGG c-myc 3’UTR mRNA
4
BGG c-myc 3’UTR mRNA GST-CAT mRNA oligo(dT)/ RNase H pHA-CMV5- CPEB Time (h) 0 0 1.5 3 1 2 345 6 7 8 9 10 Ogami_Fig. S1
description
Ogami_Fig . S1. pHA-CMV5-. CPEB. 0. Time (h). 0. 1.5. 3. oligo(dT)/ RNase H. GST-CAT mRNA. BGG c-myc 3’UTR mRNA. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. Ogami_Fig . S2. A. MS2-HA -CPEB ( ). ( ). MS2-HA. HA-CPEB ( ). (kb). 6. 2. 4. 6. 8. 0. 2. 4. 6. 8. 0. 8. - PowerPoint PPT Presentation
Transcript of BGG c-myc 3’UTR mRNA
BGGc-myc 3’UTRmRNA
GST-CAT mRNA
oligo(dT)/ RNase H
pHA-CMV5-CPEB
Time (h)0 0 1.5 3
1 2 3 4 5 6 7 8 9 10
Ogami_Fig. S1
Ogami_Fig. S2
Time (h)
BGG(1-39) MS2bs mRNA
(kb)
MS2-HAMS2-HA
-CPEB ( ) HA-CPEB ( )
0 2 4 6 8 0 2 4 6 8 0 2 4 6 8
EGFP mRNA
0.6
0.8
1.0
1.5
1 2 3 4 5 6 7 8 9 10 1112 131415 16 17
A
B C
MS2-HA-CPEB
(WB: anti-HA)
GAPDH(WB: anti-GAPDH)
HA-CPEB
MS
2-H
A
(kDa)
89
63
35
MS
2-H
A-C
PE
B
HA
-CP
EB
Time (h)
0 2 64 81
10
100
RN
A le
vel (
%)
MS2-HA
HA-CPEBMS2-HA-CPEB
( )
CP
EB
3
IPanti-
Fla
g
anti-
Fla
g
pCMV-5XFlag-CP
EB
62
(kDa)
93
anti-
Fla
g
5XMyc-PARN(WB: anti-Myc)
CP
EB
3
CP
EB
935XFlag-CPEB3
(WB: anti-Flag)5XFlag-CPEB
pCMV-5XMyc-PARN
2 3 4 5 61
Ogami_Fig. S3
BA
RT
mature(a-c)
pre(b-c)
Mak
er
Ogami_Fig. S4
