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Transcript of Susanne Vrtala, PhD Department of Pathophysiology Center for Physiology and Pathophysiology Medical...
Susanne Vrtala, PhDDepartment of PathophysiologyCenter for Physiology and PathophysiologyMedical University of Vienna, Austria
Recombinant mite allergens for diagnosis and immunotherapy
in mite allergic patients
Group Biochemical function kDa IgE binding frequency
1 cysteine protease 25 80-1002 ? 14 80-1003 trypsin 25 10-1004 amylase 60 30-705 ? 14 20-706 chymotrypsin 25 40-707 ? 24-29 30-608 glutathione-S-transferase 26 10-809 collagenolytic serine protease 24 9010 tropomyosin 37 10-6011 paramyosin 103 50-8012 ? 14 5013 fatty acid binding protein 15 10-2014 vitellogenin/apolipophorin-like 177 9015 chitinase 60 7016 gelsolin 55 5017 Ca binding EF protein 53 3518 chitinase 50 6019 antibacterial peptide homologue 7 1020 arginine kinase 40 20-4021 ? 14 30
Groups of mite allergens
Isolation and characterization of Der p allergens
Baldo & Donovan, 1988
Expression and purification of the clone 30-derived allergen
Diagnosis of mite allergy
- with mite extracts, which contain a mixture of allergenic and non-allergenic components- extracts are standardized for Der p 1 and Der p 2- some other important allergens are underrepresented in mite extracts- extracts do not allow a component-specific diagnosis
Development of new diagnostic concepts with recombinant mite allergens
IgE-reactivity profiles to Der p allergens
Recombinant mite allergens for immunotherapy
Production of hypoallergenic derivatives of mite allergens with reduced allergenic activity
Immunotherapy with mite extracts
- No patient-tailored therapy is possible- sensitization to new allergens is possible- Induction of severe anaphylactic side effects is possible
Patient 1 Patient 2 Patient 3 Patient 4 mean
Der p 2 (aa 1-53) 48 66 53 50 54
Der p 2 (aa 54-129) 39 50 52 24 41
Der p 2 Hybrid 59 76 64 47 62
Der p 2 61 87 77 73 75
IgG Abs induced with rDer p 2 derivatives inhibit patients‘ IgE binding to Der p 2 wildtype
- Identification of new mite allergens
- A combination of Der p 1 and Der p 2 allows diagnosis of almost all mite allergic patients
- Production of hypoallergenic derivatives of Der p 2 with reduced allergenic activity and retained immunogenicity
Summary
Medical University of Vienna,Department of Pathophysiology,Division of Immunopathology
Rudolf Valenta
Group Immunotechnology
Margit WeghoferKuan-Wei Chen Anne Casset
Wayne ThomasTelethon Institute of Child Health Research, Perth University of Western Australia
Friedrich HorakDepartment of Otorhinolaryngology Medical University of Vienna
Peter ValentDivision of Hematology and Hemostaseology Medical University of Vienna
Walter KellerInstitute of Chemistry Karl-Franzens University of Graz
Gabrielle PauliHôpitaux Universitaires de Strasbourg
Allergy Unit
National Health Service, Rome
Adriano Mari
Marianne van HageKarolinska Institutet, Stockholm
This work was supported by grants F1803, F1805, F1809 and F1815 of the Austrian Science Fund.