Structure and function Structure and function of a viral DNA of a viral DNA gatekeepergatekeeper

Bacteriophage SPP1

Portal protein

The Dodson symposiumThe Dodson symposium

Fred Antson, YSBLFred Antson, YSBL

TRAP, trp RNA-binding attenuation TRAP, trp RNA-binding attenuation proteinprotein

Structure and Structure and function of a function of a

viral DNA viral DNA gatekeepergatekeeper

Bacteriophage SPP1

Portal protein

29SPP1

D318

E323

D327

K331

E334D338K342

D348E352

E424

ATPase

procapsid procapsid

12

ATPase portal protein DNA

SPP1 portal protein phi-29 portal protein

V347

E424N421

31 Å 21 Å

13-mer, X-ray 12-mer, EM/X-ray

12

3

112114 5 6 7 8 9 10 1112 1

36°

28.8°

Andrey Lebedev Andrey Lebedev University of YorkUniversity of YorkPhilippa SeaversPhilippa SeaversJoanne Turner Joanne Turner Miguel Ortiz-LombardiaMiguel Ortiz-LombardiaAlexey VaguineAlexey VaguineGuy DodsonGuy DodsonEleanor Dodson Eleanor Dodson

Paulo Tavares Paulo Tavares CNRS, Gif-sur-YvetteCNRS, Gif-sur-Yvette

Margret Krause Margret Krause MPI Genetik, BerlinMPI Genetik, Berlin Thomas TrautnerThomas Trautner

Elena Orlova Elena Orlova Birkbeck CollegeBirkbeck College

Juan Alonso Juan Alonso Centro National de Centro National de Biotecnologia, MadridBiotecnologia, Madrid

Acknowledgements