Stability Test Sheet
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Transcript of Stability Test Sheet
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8/6/2019 Stability Test Sheet
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14-Jul 16-Jul 18-Jul 20-Jul 22-Jul 25-Jul 28-Jul
DAY 0 2 4 6 8 11 14
PARAOXON 23.5 PTE 196.46 193.67 189.98 194.42 203.71 194.73 194.39198.15 191.91 192.91 198.29 203.00 194.91 192.80
Average 197.31 192.79 191.45 196.36 203.36 194.82 193.60
23.5 Control 229.59 230.00 229.20 225.45 228.77 227.95 228.41
4 PTE 198.04 175.09 186.80 205.38 198.19 202.14 190.69
198.96 192.66 194.76 195.18 204.59 197.88 200.13
Average 198.50 183.88 190.78 200.28 201.39 200.01 195.41
4 Control 230.22 230.00 230.19 230.36 229.69 227.82 229.03
-15.5 PTE 191.25 190.45 173.62 202.96 200.00 201.86 199.79
202.84 187.42 191.05 197.84 198.98 200.98 197.07
Average 197.05 188.94 182.34 200.40 199.49 201.42 198.43
-15.5 Control 228.48 231.03 232.38 226.98 229.84 229.47 230.69
PARATHION 23.5 PTE 204.50 190.67 193.72 183.20 202.10 196.80 204.51
197.23 195.85 193.84 202.23 208.75 198.57 201.43 Average 200.87 193.26 193.78 192.72 205.43 197.69 202.97
23.5 Control 233.95 231.30 229.17 225.33 227.47 227.65 227.02
4 PTE 197.78 196.38 195.10 205.60 202.69 204.44 205.70
202.40 194.57 192.47 197.51 196.67 199.43 201.30
Average 200.09 195.48 193.79 201.56 199.68 201.94 203.50
4 Control 229.64 231.81 229.55 231.86 231.23 230.50 230.60
-15.5 PTE 204.30 194.18 181.98 203.55 197.90 209.55 186.71
197.63 185.69 191.01 199.48 186.68 206.12 204.59
Average 200.97 189.94 186.50 201.52 192.29 207.84 195.65
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8/6/2019 Stability Test Sheet
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Notes:
- Day 0 colour tested after 15 min incubation at approx. 5:30pm, thereafter colour allowed to develop over 15 mi- Paraoxon and parathion blue light value in ImageJ used to represent change in yellow colour
- Coumaphos red, green, blue light colour values recorded to detect blue fluoresence change
- For coumaphos the photos taken typically show up a bright white blue fluorescence and in Image J the green
DAY 0 2 4 6 8 11 14
Coumaphos 23.5 PTE 1 1 1 1 1 1 1
(qualitative) 23.5 control 0 0 0 0 0 0 0
4 PTE 1 1 1 1 1 1 1
4 control 0 0 0 0 0 0 0
-15.5 PTE 1 1 1 1 1 1 1
-15.5 control 0 0 0 0 0 0 0
- Using PTE purified from Pseudomonas putida, with pVLT-31 + opdgene
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8/6/2019 Stability Test Sheet
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31-Jul 4-Aug 9-Aug 13-Aug 18-Aug 23-Aug
17 21 26 30 35 40
196.10 197.93 193.85 196.72 196.41 188.04192.66 188.64 199.75 196.09 193.03 188.35
194.38 193.29 196.80 196.41 194.72 188.20
224.54 222.79 226.70 225.05 224.32 226.21
190.32 203.75 188.61 180.75 198.13 184.84
189.32 202.54 191.44 199.48 188.57 187.27
189.82 203.15 190.03 190.12 193.35 186.06
226.92 220.26 224.17 224.68 221.89 218.04
196.33 187.53 192.14 195.60 199.52 174.81
191.97 202.43 196.90 196.72 198.69 187.26
194.15 194.98 194.52 196.16 199.11 181.04
229.15 223.69 226.84 224.75 228.58 221.77
208.39 199.36 199.67 190.40 180.85 191.69
202.02 197.43 197.01 197.73 195.90 -205.21 198.40 198.34 194.07 188.38 191.69
228.31 224.65 226.00 225.48 222.73 231.37
205.31 204.14 200.40 204.23 201.69 180.73
189.30 201.16 201.92 205.20 203.97 191.54
197.31 202.65 201.16 204.72 202.83 186.14
227.72 224.72 220.64 229.29 226.21 225.23
193.31 201.16 189.20 204.19 193.73 173.90
203.22 197.06 196.14 204.75 202.51 -
198.27 199.11 192.67 204.47 198.12 173.90
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8/6/2019 Stability Test Sheet
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in. Day 2 and 4 were incubated longer, approx 30 min
nd blue values max out at 255.
Preformed by Chris Langley and
17 21 26 30 35 40
1 1 1 1 1 1
0 0 0 0 0 0
1 1 1 1 1 1
0 0 0 0 0 0
1 1 1 1 1 1
0 0 0 0 0 0
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8/6/2019 Stability Test Sheet
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avid Collings beginning July 14th, 2010
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8/6/2019 Stability Test Sheet
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8/6/2019 Stability Test Sheet
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0 10 2 0 30 4 0
170.00
180.00
190.00
200.00
210.00
220.00
230.00
240.00
Immobilized PTE Stability Test - 1mM Paraoxon
23.5 PTE 4 PTE -15.5 PTE 23.5 Control 4 Control -15.5 Control
Days
Meanblueh
istog
ram
value
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8/6/2019 Stability Test Sheet
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0 10 2 0 30 4 0
170.00
180.00
190.00
200.00
210.00
220.00
230.00
240.00
Immobilized PTE Stability Test - 1mM Parathion
23.5 PTE 4 PTE -15.5 PTE 23.5 Control 4 Control -15.5 Control
Days
Meanbluehistog
ram
va
lue
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0 5 10 15 20 25 30 35
0.00
50.00
100.00
150.00
200.00
250.00
300.00
1mM COUMAPHOS Blue
-15.5 Control Row 36 Row 41 Row 46 Row 42 Row 37
Days
Meanbluedetection
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0 5 10 15 20 25 30 35 40
-0.2
0
0.2
0.4
0.6
0.8
1
1.2
Immobilized PTE Stability Test - 1mM Coumaphos (Qualitative)
23.5 PTE
4 PTE
-15.5 PTE
23.5 control
4 control
-15.5 control
Days
Visiblefluorescenceun
derUVhandheld