Specimen Requirements

Heparin - green top EDTA - purple top Storage range 4°C = 2 - 8°C Room Temp = 18 - 25°C (Minimum volumes do not allow for retest) Rev 13JUL2017 | Page 1 of 4

For a complete test menu and specimen requirements please visit www.mplnet.com or call Client Services at 800.932.2943

Test Name (Test Code) Specimen(s) Volume (min.) Anticoag/ Medium Preferred (accepted)

Storage Stability Shipping Special Instructions

Flow CytometryWhole blood 4mL (1mL)

Bone marrow aspirate 2mL (0.5mL)

Sodium Heparin (EDTA)

BM core biopsy 2cm (1cm)

Fresh tissue 5mm3

FNA 4mL (0.5mL)

Sterile container, addition of 2-4mL transport media

Leukemia / myeloma / lymphoma analysis

Fluids, CSF, pleural, synovial, pericardial fluids

4mL (0.5mL) Sterile container, addition of 2-4mL transport media

Paroxysmal nocturnal hemoglobinuria; Leukocyte adhesion deficiency; ZAP-70

Whole blood 5mL (0.5mL) Sodium Heparin (EDTA)

Bronchoalveolar lavage lymphocyte subsets

Bronchoalveolar lavage 10mL (5mL) Sterile container, addition of 2-4mL transport media

4°C 48hrs Ice pack Protect from extreme temperatureSeparate ice pack from specimen

Pathology & ImmunohistochemistryAspirate 4mL (0.5mL) Sodium Heparin (flow and

cytogenetics; EDTA molecular studies)

RT 48 hrs Ambient

Core biopsy 1cm Formalin or B-Plus

Aspirate clot 5mm3 Formalin or B-Plus

Aspirate smear 4-6 slides Air dried

Bone marrow pathology evaluation(All specimen types required)

Blood smear 1 slide Air dried

RT Indefinite Ambient

Use hematopathology collection kit and include CBC report

Paraffin embed tissue Block Formalin fixedImmunohistochemistry markers

Slides; each marker/analyte – cut at 3μ and centrally located on “plus” slides

3 slides Air dried

RT Indefinite Ambient Protect from extreme temperature

Specimen Requirements

Heparin - green top EDTA - purple top Storage range 4°C = 2 - 8°C Room Temp = 18 - 25°C (Minimum volumes do not allow for retest) Rev 13JUL2017 | Page 2 of 4

For a complete test menu and specimen requirements please visit www.mplnet.com or call Client Services at 800.932.2943

Test Name (Test Code) Specimen(s) Volume (min.) Anticoag/ Medium Preferred (accepted)

Storage Stability Shipping Special Instructions

Molecular Oncology

Whole blood 5mL (3mL) EDTA or Sodium Heparin RT 72hrs AmbientHematological - NGSIgVH somatic hypermutationMyeloproliferative panelsCALR mutationJAK2 Exon 12 mutationMPL mutationMYD88 mutation

Bone marrow 3mL (1mL) EDTA or Sodium Heparin RT 72hrs Ambient

Paraffin embed tissue Block or 3-5 Slides 5um min

Formalin fixed RT Indefinite Ambient

Whole blood 5mL (3mL) EDTA or Sodium Heparin RT 72hrs Ambient

Solid Tumor- NGS

EGFR, KRAS, BRAF, IDH1/2 Genes

Colon, Lung, Melanoma, GIST panels

8 gene Solid Tumor panels

Bone marrow 3mL (1mL) EDTA or Sodium Heparin RT 72hrs Ambient

Protect from extreme temperature with ice pack.Do not allow the ice pack to directly contact the sample.Protect from extreme temperature with ice pack.Do not allow the ice pack to directly contact the sample.

Whole blood 5mL (3mL) EDTA or Sodium Heparin RT 72hrs Ambient

Bone marrow 3mL (1mL) EDTA or Sodium Heparin RT 72hrs Ambient

BM core biopsy 5mm3

Fresh tissue 5mm3

Fine needle aspirate 3mL (2mL)

4°C 72hrs Ice pack

Protect from extreme temperature with ice pack.Do not allow the ice pack to directly contact the sample.

Frozen tissue 5mm3

Sterile container, addition of 2-4mL transport media

-20°C Indefinite Dry ice Freeze immediately

PCR TestingB cell IgH heavy chain gene rearrangementB cell IgH kappa light chain gene rearrangementBCL2 t(14;18) gene rearrangement T cell receptor gamma gene rearrangement KIT (D816V) mutations

Paraffin embed tissue Block or 3-5 Slides 5um min

Formalin fixed RT Indefinite Ambient No heavy metal fixatives

Whole blood 5mL (3mL)KIT mutations AMLAML Mutation profile: FLT3, NPMI; CEBPA mutation, JAK2 V617F mutation

Bone Marrow 3mL (1mL)

EDTA or Sodium Heparin RT 72hrs Ambient

Whole blood 10mL min.ABL Kinase gene mutationBCR/ABL major p210 and minor p190 transcriptsPML-RARA t(15;17) short and long form

Bone marrow 2mL (1ml)

EDTA 4°C 48hrs Ambient

Protect from extreme temperature with ice pack.Do not allow the ice pack to directly contact the sample.

Specimen Requirements

Heparin - green top EDTA - purple top Storage range 4°C = 2 - 8°C Room Temp = 18 - 25°C (Minimum volumes do not allow for retest) Rev 13JUL2017 | Page 3 of 4

For a complete test menu and specimen requirements please visit www.mplnet.com or call Client Services at 800.932.2943

Test Name (Test Code) Specimen(s) Volume (min.) Anticoag/ Medium Preferred (accepted)

Storage Stability Shipping Special Instructions

Cytogenetics - OncologyWhole blood 7mL (5mL)

Bone marrow 3mL (1mL)

Sodium Heparin

BM core biopsy or fresh tissue

5mm3

Chromosome analysis

Fine needle aspirate 4mL (2mL)

Sterile container, addition of 2-4mL transport medium

RT 48hrs Ambient Protect from extreme temperature with ice pack.Do not allow the ice pack to directly contact the sample. For cancer cytogenetics please send WBC count with requisition. Call before sending specimen on Friday. Ship on Saturday for Monday delivery only.

Whole blood 5mL (3mL)

Bone marrow 3mL (1mL)

Sodium Heparin (EDTA) RT 72hrs Ambient

Fine needle aspirate 3mL (2mL)

Fluorescence in situ hybridization (FISH) probes for hematological disorders

Fresh Tissue 5mm3

Sterile container, addition of 2-4mL transport medium

4°C 72hrs Ice pack

BlockFluorescence in situ hybridization (FISH) probes for solid tumors

Paraffin embed tissue

3-4 Slides, 5 microns

Formalin fixed RT Indefinite Ambient

Protect from extreme temperature with ice pack.Do not allow the ice pack to directly contact the sample.

Bladder Cancer FISH Urine 50mL (35mL) PreservCyt® 4°C - 30°C 48hrs Ship overnight.

Molar pregnancy: 13+/18+/21+/X/Y

FFPE 3-4 Slides, Blocks Formalin fixed RT Indefinite Ambient Protect from extreme temperature

Cytogenetics - Constitutional

Newborn blood 1mL min Sodium Hep (ped tube)

Whole blood 7mL (5mL) Sodium Hep

Chromosome analysis

Umbilical blood 2mL (1mL) Sodium Hep

RT 48hrs Ambient

Solid tissue chromosome analysis

POC, chorionic villus, fetal tissue

Tissue, Skin biopsy

Sterile container, addition of 2-4mL transport media

RT 48hrs Ambient

Protect from extreme temperature. Call before sending specimen on Friday. Ship on Saturday for Monday delivery only if sample is protected from extreme temperature. Include gestational age and due date.

Specimen Requirements

Heparin - green top EDTA - purple top Storage range 4°C = 2 - 8°C Room Temp = 18 - 25°C (Minimum volumes do not allow for retest) Rev 13JUL2017 | Page 4 of 4

For a complete test menu and specimen requirements please visit www.mplnet.com or call Client Services at 800.932.2943

Test Name (Test Code) Specimen(s) Volume (min.) Anticoag/ Medium Preferred (accepted)

Storage Stability Shipping Special Instructions

Medical GeneticsRT 72hrs AmbientCystic fibrosis Luminex xTAG 39 Whole blood 5mL (3mL) EDTA

4°C 30 days Ice pack Protect from extreme temperature Separate ice pack from specimen

RT 72hrs AmbientCystic fibrosis MiSeqDx 139 Variant Assay by NGSCystic fibrosis MiSeqDx Clinical Sequence Assay by NGS

Whole blood 5mL (3mL) EDTA

4°C 30 days Ice pack Protect from extreme temperature Separate ice pack from specimen

Infectious DiseaseCervical cells 3mL ThinPrep® or SurePath™ 2-30ºC 21 days Ambient

Cervical or urethral swab One swab APTIMA Unisex Swab Specimen Collection

2-30ºC 60 days Ambient

Urine 20-30mL Preservative-free urine collection cup

4°C 24hrs Ice pack Void first 10mL

Chlamydia trachomatis Neisseria gonorrhoeae

Urine 2mL APTIMA urine tube RT 30 days Ambient

HPV high risk by AptimaTrichomonas Vaginalis

Cervical cells 2mL(1mL) ThinPrep® or SurePath™ RT 21 days Ambient

HPV genotyping Liquid cytology 2mL ThinPrep® or SurePath™ RT 21 days Ambient

HSV lesion viral transport media 4°C 96hrs Ice pack Separate ice pack from specimen

Whole blood 2mL EDTA 4°C 96hrs Ice pack Separate ice pack from specimen

Fresh Tissue 5mm3 4°C 96hrs Ice pack Separate ice pack from specimen

Frozen tissue 5mm3

Sterile container, addition of 2-4mL transport medium -20°C ~ Dry ice Freeze immediately

Swab from any site viral transport media 4°C 96hrs Ice pack Separate ice pack from specimen

4°C 72hrs Ice packCSF 2mL (1mL) Sterile container

-20°C ~ Dry ice Freeze within 4 hrs

Herpes Simplex Type I/2 qualitative by real-time PCR

Cervical cells 2mL (1mL) ThinPrep® or SurePath™ RT 21 days Ambient