Smah Abdelhamid. Its an analytical techniques that couples chromatographic and spectral methods to...
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Transcript of Smah Abdelhamid. Its an analytical techniques that couples chromatographic and spectral methods to...
Hyphenated Techniques
Smah Abdelhamid
Its an analytical techniques that couples
chromatographic and spectral methods to exploit the advantages of each Chromatography: identify chemicals purely or
nearly purely in complex mixtures. Spectroscopy: yields qualitative information
about pure component. Introduce to the scientific community in 1980,
by Dr. Tomas Hirschfeld an analytical chemist
What?
Highly selective Highly sensitive Use in many Applications
purifications Polymerization studies Natural products Pharmaceutical Many more….
Why?
Very sensitive and selective Most common Sample is injected into injection port of GC,
than its vaporized, separated in the GC column, than Analyzed by MS detector, and recorded
Provides structural information Volatile compounds Polar compounds
GC-MS
GC-MS
Applications Environomental
monitoring and cleanup organic pollutant
Criminal forensics Drug detections Fires investigations Explosive investigations
Sports Medicine
Double three way diverter in line with an auto
sampler, and LC system, and the MS Second most common Poor structure determination
LC-MS-MS Increase fragmentation
LC-UV-MS Biologhical screening for natural products
LC-MS
LC-MS
Applications
Pharmacokinetics Bioanalysis
Proteomics Metabolomics Drug development
Least sensitive High cost Provide best structural information Analysis of natural products Analysis of drug related metabolites in bio-fluids
and biomolecules in general analysis of organic molecules Drug impurities Reaction mixtures Drug degradation products
HPLC-NMR
HPLC-NMR
Autosampler, LC pump, Column, a non-NMR detector
Flow is guided in the LC-NMR interface
Than its guide it to the Flow-cell NMR probe Head
Following that the flow is routed to a fraction collector for recovery or further invegistation.
CE: Capillary Electrophoresis
Analysis is driven by an electric filed in narrow tubes
Results in fast separation of compounds (almost all compounds can be separated through this methods)
Analysis of purities Assays Analysis of trace level determination
CE-MS
CE-MS
CE-UV GC-FTIR
Identifying isomers Ephendrine-type alkaloid found in dietrary
supplements containing the chinese herb ma huang
HPLC-ESI-MS HPLC-PDA
Analysis of coumarins
Other Hyphenated Techniques
Spectra
GC-MS LC-MS
Spectra
HPLC-NMR CE-MS
Conclusion
Since the introduction of hyphenated techniques in the 1980s they under went great evolution due to technology, which has expand its use and applications.
1. Patel, K., Patel, J., Patel, M., Rajput, G., & Patel, H.
(2010). Introduction to hyphenated techniques and their applications in pharmacy. Pharmaceutical Methods, 2(1), 2. doi:10.4103/2229-4708.72222
2. The Hy-phen-ated MethodsTomas HirschfeldAnalytical Chemistry 1980 52 (2), 297A-312A
3. http://www.sigmaaldrich.com/analytical-chromatography/analytical-standards/application-area-technique/standards-for-iso-188572.html (images)
4. http://www.nature.com/nchembio/journal/v2/n8/fig_tab/nchembio803_F4.html
Reference