Shantani Proteome Analytics Pvt. Ltd.

Advancing Technologies and Applications of Proteome Analysis

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Advancing Technologies and Applications of Proteome Analysis For Drug Discovery and Development Needs

Target Identification Technologies

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Importance of Target and MOA Information

All in all Target and MOA information allows making a ‘GO / NO GO’ drug development decisions !!!

Discovery Lead Pre-clinic Clinical Market

On / Off Target

Identification

Mode of Action Analysis

Toxicity Profiling

Biomarker Identification

Drug Repositioning

Patient Stratification

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Our Domain - Small-Molecule Target Identification

Assay Development Target & MOA Screening Lead Molecule Shantani’s Target ID

Technology

Phenotype / Cell Based Drug Screening

Off-Target Info Screening Lead Molecule

Target Based Drug Screening

Target

New Indications & Primary Target Info New Targets & MOA

Drug Repositioning

Existing Drug

Lead Optimization

Scientific Basis for New Indications

Toxicity Profile & Lead Optimization

Shantani’s Target ID

Technology

Shantani’s Target ID

Technology

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Small molecule Drug Target Identification – Shantani’s Technological Solutions

Long list of targets

Prioritization of Targets is cumbersome

Validation of long list is time and cost consuming

Existing Target Identification Methods

Identification of few but rightful targets

30-50 % savings in validation exercises by validating only rightful targets

Shantani’s Target Identification Technologies

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Shantani’s Target Identification Technology

Target proteins are identified for unmodified small-molecules

Target proteins are captured from intact, physiologically relevant biological systems

Small-molecule target interactions can be directly quantified in cell-based systems

Method is not limited to ‘Kinase’ or any particular class of proteins

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Shantani’s Target Identification work flow

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Technical Overview of Target Validation Process

Physical interaction of the test compound with the identified target proteins – By utilizing appropriate biophysical methods (ITC, Fluorescence)

Functional responsiveness of the target proteins – By utilizing modern molecular biology (siRNA) and pharmacological methods

Mechanistic interaction details / MOA – ‘in silico’ analysis combined with wet-lab experiments to obtain the information on target and related pathways and its association with the disease state

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We Offer 3-Tier Small-molecule Target Identification Services

Target ID Methods Key Points of Considerations

Typical Numbers of Deconvoluted

Targets

Quantitative Analysis of Small-molecule target

Interaction

Target Validation in Cellular

Models

Action Mechanism

Studies

Level 1

Fast and inexpensive, Good for comparative data analysis if the target id data is available from other methodologies, also good for generating crude information about the proteome to which small molecule interacts

~ 25-30 None (unless specified)

None (unless specified) None

Level 2

More specific target information then level 1, Fast, may be utilized as stand-alone method for target identification

~12-18 None (unless specified) Need Based 'in-silico' studies

Level 3

Shantani's advanced technology, cellular location specific target capture from live cells, in-depth target information then level 2, can be utilized as stand-alone method for target identification, allows clear Go/ No Go decision making

~4-8 For almost all identified targets

For almost all identified targets

'in-silico' studies + wet lab validation

Innovative Cell Based Assays

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Customized Assay Development

Scientific expertise available to Shantani allows it to think out-of-box to analyze biomolecular interactions

Customer’s Pain Points

Discussions with Team of Shantani

Development of ‘fit-for-purpose’

assay

Product Development

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Development of Novel Cell-Based Assays

No direct target-engagement information

Time consuming

Suffer from data-artifact due to cell lysis

Existing Cell Based Methods

Direct target-engagement information

Generating multiple information from the same assay Cell permeability of

the molecule Cell based Ki

Can be customized

Shantani’s Novel Cell Based Assay

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Examples : Novel Cell Based Assay

Concept Design Implementation

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Shantani’s Cell Based Assay Capabilities

Development of novel cell based assays Routine performance of following assays

Cell Proliferation Apoptosis Membrane Integrity Assay Oxidative Stress

Protein Analytical Services

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Better, Faster and Cost-Effective Protein Analysis Services Quantitative Proteomics

Protein / Peptide quantification in biological matrix (SRM / MRM) Label-free semi quantitative analysis

Protein Structural Analysis Primary Structure and PTMs

Mass Spectrometry, Chromatography, Capillary Electrophoresis, N-terminal sequencing Higher Order Structures

Fluorescence Spectroscopy, FT-IR, DSC, AUC, CD

Protein Purity and Potency Assessments Size, Charge and Hydrophobicity

Size Exclusion, Ion-exchange, Reveres Phase Chromatography and Isoelectric Focusing, AUC

Impurity HPLC, SDS-PAGE, AUC

Binding / Bioassay

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‘Biosimilar’ Comparability Analysis at Shantani

Structure

Purity / Efficacy / Potency

20 structure

30 structure

40 structure

Immunogenicity / Toxicity

Size / Charge / Hydrophobilicity

Glycosylation

Bioassay / Binding Assay

Cell Based Models

Animal Models

10 structure

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Working with Clients: Overview

Step 0: Interest from Clients Step 1: Signing of CDA (if needed) and sharing project information Step 2: Based on the project Shantani sends quotation detailing

milestones, timelines and deliverables Step 3: Client revises/accepts the quotation and Shantani initiate the

project Step 4: Shantani provides milestone based reports Step 5: Shantani and Client agrees on the final outcome, project

considered close (Data support stays for another 12 months)

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Shantani’s Working Philosophy

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Our Capabilities

Laboratory Infrastructure: Resident of well-equipped Venture Center of National Chemical Laboratory, Pune, India.

Human Resources: Internationally trained and experienced drug discovery technologists, scientists and business professionals

Scientific rigor and customer focused attitude are our biggest assets.

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Quality for non-regulate work / Facility

~1380 square feet of lab space (divided into 5 shared instrument facility rooms) housing different instruments and served by a common corridor

~ 300 square feet of personal work bench space Each lab room comply local safety regulations Lab blocks are guarded by double layers of security Well practiced Environment Health & Safety (EHS) policies Data is generated from routinely calibrated instruments Data is analyzed and stored in e-lab notebooks providing fast

retrieval

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Organizational Strength

Well defined organizational structure Standard Operating Protocols (SOPs) and QC & QA

processes in place Documented protocols to protect intellectual properties Standardized material and data management processes Strong IT policies for data management (storage and

backup) including data sharing processes

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Snapshots of the Available Facilities

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Queries are Welcome and Thank You !!!

Shantani Proteome Analytics Pvt. Ltd. No Superlatives just good work

100 National Chemical Laboratories Innovation Park Dr. Homi Bhabha Road, Pune, MH, India, 411 008 Email: bd@shantani.com Phone: +91 997 544 7489 http://www.shantani.com