Curriculum vitae Tina Šantl-Temkiv, PhD Department of Bioscience, Aarhus University, Ny Munkegade 116, DK-8000 Aarhus Date of birth: 10. Feb 1983, Nationality: Slovene e-mail: temkiv@phys.au.dk Mobile: +45 29886984 Orcid ID: 0000-0002-3446-5813

Curriculum vitae

1. Professional Appointments

Dec 2019 –

Assistant Professor (tenure track) Dept. of Biology, Aarhus University, Denmark. Scientific focus Investigating the activity status of airborne bacteria in connection to their impact on atmospheric water and element cycles. Polar atmospheric microbiology. Structure and function of ice nucleation proteins. Techniques used Molecular microbial ecology: mRNA and rRNA work; RT-qPCR; next generation sequencing: amplicon sequencing, metagenomics, metatransriptomics; next generation sequence analysis (DADA2, R software package); gene expression analysis using reporter strains and gene deletions. Classical microbiology: TEM; cultivation of aerobic heterotrophs. Single cell techniques: Flow cytometry, Fluorescence microscopy, Raman microspectroscopy. Atmospheric science: droplet-freezing assays; optical particle sizer (OPS); sparging liquid aerosol generator (SLAG); BioSampler; atmospheric chambers for microbial experiments: sea-spray tanks; flow tubes; single particle levitation chambers; filter and impinger sampling; BIOTRAK Real-Time Viable Particle Counter.

Jul 2016 –

Assistant Professor Dept. of Bioscience and Dept. of Physics and Astronomy, Stellar Astrophysics Centre (SAC), Aarhus University, Denmark. Scientific focus Investigating the activity status of airborne bacteria in connection to their impact on atmospheric water and element cycles. Polar atmospheric microbiology. Structure and function of ice nucleation proteins. Techniques used Molecular microbial ecology: mRNA and rRNA work; RT-qPCR; next generation sequencing with Illumina MiSeq; next generation sequence analysis (Mothur, DADA2, R software package); gene expression analysis using reporter strains and gene deletions. Classical microbiology: TEM; cultivation of aerobic heterotrophs; droplet-freezing assays. Single cell techniques: Flow cytometry, Fluorescence microscopy, Raman microspectroscopy. Atmospheric science: PTR-(TOF)-MS; optical particle sizer (OPS); sparging liquid aerosol generator (SLAG); BioSampler; atmospheric chambers for microbial experiments: sea-spray tanks and flow tubes; filter and impinger sampling; BIOTRAK Real-Time Viable Particle Counter.

Jul 2012 – Jun 2016

PostDoc Dept. of Physics and Astronomy, Stellar Astrophysics Centre (SAC), Aarhus University, Denmark. Scientific focus Biosignatures associated with airborne bacteria, in particular their influence on the cloud formation and the precipitation patterns. Techniques used Molecular microbial ecology: mRNA and rRNA work; developing qPCR assays; RT-qPCR; PCR; cloning; next generation amplicon sequencing with IonTorrent; next

Scientific focus areas § The activity and ice-nucleation activity of

atmospheric microorganisms § The impact of microorganisms on atmospheric

processes § Methods for simulating atmospheric conditions in the

laboratory and for sampling airborne microorganisms § Arctic microbiology: coupling between marine,

terrestrial and atmospheric environments

Research achievements in numbers 20 papers in peer-reviewed journals, 4 peer-reviewed conference abstracts, 1 book chapter (580 citations, H-index 11; Google Scholar, Jan 2020). 19 invited talks at national and international conferences or institutions.

generation sequence analysis (Mothur, R software package). Classical microbiology: TEM; cultivation of aerobic heterotrophs; ice nucleation phenotypic assays. Single cell techniques: Flow cytometry, Fluorescence microscopy, Raman spectroscopy. Atmospheric science: PTR-(TOF)-MS, OPS, sparging liquid aerosol generator (SLAG), BioSampler, filter and impinger sampling.

Mar 2015 – Mar 2016

PostDoc Ergonomics and Aerosol Technology, Department of Design Sciences, Faculty of Engineering LTH, Lund University, Sweden. Scientific focus Bacterial aerosolization and its effects on bacterial survival and viability. Development of techniques for sampling and spraying of bioaerosols. Techniques used Atmospheric science: Sparging Liquid Aerosol Generator (SLAG), Scanning Mobility Particle Sizer Spectrometer (SMPS); Aerodynamic Particle Sizer (APS); Portable Outdoor Dust Monitor (ODM), BIOTRAK Real-Time Viable Particle Counter, development of a high-volume impinger for sampling airborne bacteria. Single cell techniques: Flow cytometry.

Nov 2011 – Dec 2011

Transition PostDoc Geological Survey of Denmark and Greenland (GEUS), Copenhagen, Denmark

Nov 2008 – Oct 2011

PhD candidate National Environmental Research Institute (NERI), Aarhus University, Roskilde, Denmark. Scientific focus The diversity and activity of airborne bacteria and their influence on atmospheric chemistry. Techniques used Analytic chemistry: gas chromatography. Molecular microbial ecology: working with mRNA; decontaminating procedures for highly sensitive PCR; PCR; qPCR; RT-qPCR; nested PCR; cloning; sequence analysis; fingerprinting (ARDRA, RADP, ITS). Classical microbiology: isolations of aerobic heterotrophs, phenotype screening (Biolog plates); TEM; enrichment cultures; ice nucleation phenotypic assays; enumeration of INA bacterial cells (MPN method). Single cell techniques: FISH. Atmospheric science: development of an aircraft-operated impactor for sampling cloud water.

Sep 2007 – Sep 2008

Science teacher, QSI International School of Ljubljana, Slovenia High school subjects taught Microbiology, Biology, Life science, Physical science and Chemistry

2. Academic Degrees

May 25, 2016

MSc degree in Biology (Pedagogy module) University of Ljubljana, Biotechnical faculty Average grade: 8.8 (out of 10)

Feb 3, 2012 PhD degree, Microbial ecology of the atmosphere University of Aarhus PhD thesis: Bacteria in clouds Advisors: Ulrich Gosewinkel, Bjarne M Hansen and Kai Finster Committee: Birgit Sattler, Pierre Amato and Philipp Mayer

Sep 25, 2008

MSc degree in Biology (Molecular biology module) University of Ljubljana, Biotechnical faculty Average grade: 8.7 (out of 10); Master thesis grade (thesis grade/thesis defence grade): 10/10 (out of 10/10) MSc thesis: Home range of whinchat (Saxicola rubetra) during its nesting period

Oct 8, 2007

MSc degree in Cultural Studies University of Ljubljana, Faculty of Social Sciences Average grade: 9.0 (out of 10); Master thesis grade (thesis grade/thesis defence grade): 10/10 (out of 10/10) MSc thesis: Contemporary conceptions of time

Oct 2004 – Sep 2005 Exchange program I finished one year of my M.sc. program through the European Commission

exchange (Erasmus) programme at the University of Vienna and the University of Natural Resources and Life Sciences (BOKU), Vienna, Austria

3. Language skills

Mother tongue: Slovenian (primary), Serbian and Rusinian (secondary). Self-assessment based on Common European Framework of Reference for Languages Understanding Speaking Writing Languages Listening Reading Spoken interaction Spoken production English C1 Proficient user C1 Proficient user C1 Proficient user C1 Proficient user C1 Proficient user German B2 Independent user B2 Independent user B1 Independent user B1 Independent user B1 Independent user Danish B2 Basic user B1 Independent user A1 Basic user A1 Basic user A2 Basic user Rusinian A2 Basic user A2 Basic user A1 Basic user A1 Basic user A1 Basic user Croatian/Serbian C1 Proficient user C1 Proficient user C1 Proficient user B2 Independent user B1 Independent user

4. Personal skills - Due to a long record of interdisciplinary work, I am good at communicating research to scientists

from other fields and managing interdisciplinary projects. - I have a vast ability to adapt to multicultural environments gained through 14 years of working and

studying abroad in Austria, Denmark, and Sweden, as well as through frequent travelling in Europe, Africa, Asia, and central, north and south America.

- I am highly capable of knowledge transfer, which is evident from my extensive mentoring activities, publication and outreach record, as well as presentations at scientific conferences.

- I am good at working independently as well as at teamwork. Already during my PhD, much of my research was very independent and involved taking care of my project budget as well as designing my own studies, which led to three out of four first-author publications. I am also good at teamwork, due to the numerous collaborations, and working together with researchers, laboratory technicians, graduate, and undergraduate students.

- I acquired organizational and fundraising skills firstly through organizing several projects firstly, during my work as a volunteer in several non-profit organizations: Junior Research Association at AU, Biology Students’ Society; Society of Students of Cultural Studies, kult.co (as a vice-president); and SHS, Societas herpetologica slovenica at the University of Ljubljana and secondly, during my postdoc and assistant professor positions applying for funding as PI and co-PI, as well as acquiring management roles in MILAF and Atmospherica interdisciplinary networks.

5. Current Research Support - Novo Nordisk Foundation (2020-2025), co-PI, 15 mill DKK - DFF-Danish ERC Programme (2019-2021), PI, 1.2 mill DKK - Villum Experiment (2019-2021), PI, 2 mill DKK; - COST Action (2019-2022), Horizon 2020, co-applicant; - ERC Starting Grant (2019-2024), co-applicant, 11.2 mill DKK, share of grant: 0.9 mill DKK; - 2017-2022, Norwegian research council, project partner, 11 mill DKK, share of grant: 0.9 mill DKK; - Icelandic Research Fund (2018-2022), conceived the original idea, written the proposal, co-applicant,

3 mill DKK; - Faroese Research Grant (2019), co-applicant, 0.5 mill DKK; - São Paulo Research Foundation (2018-2023), co-applicant, 16 mill DKK.

6. Awards, Grants and Scholarships - Ministry for Education, Science and Sports of Slovenia Internationalization Grant (2020), 3.5-months

pedagogical stay at the University in Nova Gorica, Slovenia, ~150,000 DKK - Villum Young Investigator (2019), PI, 10 mill DKK (second stage, interview); - ERC Starting Grant (2019), PI, 11.2 mill DKK (second stage, interview);

- The Europlanet 2020 Research Infrastructure (2018), Horizon 2020; - AU Interdisciplinary Network Grant Scheme (2017-2018), AUFF, project leader, 0.4 mill DKK; - International Conference on Polar and Alpine Microbiology (2017), Carlsberg Foundation, co-

applicant, 60,000 DKK; - The AU Research Foundation (2016-2019), conceived the original idea, co-applicant, 3 mill DKK; - Rannis postdoctoral research grant (2016), Icelandic Research Fund, PI, share of grant: 1.5 mill DKK; - RECETO PostDoc grant, University of Copenhagen (2011), main applicant, share of grant: 40,000

DKK; - Aarhus University Research Foundation travel grant (2009), main applicant, share of grant: 20,000

DKK; - Prešeren Faculty Award for an outstanding Master Thesis, University of Ljubljana (2007); - European Commission scholarship for international student exchange (2004/05); - Zois scholarship (1997-2008), Slovene Human Resource Development and Scholarship Fund.

7. Management experience - Co-PI on the Novo Nordisk Interdisciplinary Synergy Project "Deciphering the Role of Atmospheric

Microbial Aerosols (DRAMA)"; - PI on the DFF-Danish ERC Programme project “MIMOSA” (2019-2021); - PI on the Villum Experiment project “Deciphering the role of sea-ice microorganisms on cloud

processes in the Arctic” (2019-2022); - Team member for the DANWISE (Danish Society for Women in Science) (2019); - Board member and Treasurer for the Junior Research Association, Aarhus University (2018-2019); - Head of the Interdisciplinary Network Atmospherica (2017-2019); - Member and a part of the scientific committee of the Microbes at the Interface of Land-Atmosphere

Feedbacks (MILAF) network (2014-2017); - Member of the organizing and the scientific committee of the 7th International Conference on Polar

and Alpine Microbiology (2017); - Member of the VIP group at the Microbiology Section, Department of Bioscience (2016-present). - Management of research expeditions / field sites : establishing a field site in Roskilde, DK (2008-

2009), sampling campaign at the Nuuk Basic Research Station, Greenland (2013), Cruise with the Danish Navy patrol vessel Ejnar Mikkelsen, west Greenland (2016), 5 sampling campaigns at the Villum Research Station (2015-present), sampling campaign at the Juan Carlos I Antarctic Base, Antarctica (2019).

8. Management of research expeditions 2015–

Managing and/or conducting 5 sampling campaigns at the Villum Research Station, Greenland

Jan-Dec 2020 Managing a sampling campaign at the Comandante Ferraz Antarctic Station, Antarctic (collaboration with Prof. Paulo Camara, University of Brasilia, Brazil)

Feb 2019 Managing and conducting a sampling campaign at the Juan Carlos I Antarctic Base, Antarctic (collaboration with Dr. Manuel Dall’Osto, Institute of Marine Sciences, Barcelona, Spain)

2016 – 2017 Managing and conducting an aircraft sampling campaign with Cessna model 172, Denmark

Aug 2016 Managing and conducting a sampling cruise in Baffin Bay with Danish Navy patrol vessel Ejnar Mikkelsen

Jul 2013 Managing and conducting a sampling campaign at Nuuk Basic Research Station, Greenland

2008 – 2009 Establishing and maintaining a field site for a yearlong sampling campaign in Roskilde, Denmark

9. International Research Stays - Jan 2014, Institute for Astronomy, University of Hawaii, USA; - Dec 2013, Department of Design Sciences, Lund University, Sweden; - Aug, 2011, Leibniz Institute for Tropospheric Research, Leipzig, Germany.

10. Interdisciplinary experience and collaborations - International collaborations: Cloud laboratory at the Leibniz Institute for Tropospheric Research

(Leipzig, Germany); Weizmann Institute of Science (Rehovt, Israel); Institute of Marine Sciences (Barcelona, Spain); University of Brasilia (Brasilia, Brazil); University of São Paulo (São Paulo, Brazil)); Faculty of Engineering, Lund University (Sweden); University of Ljubljana (Slovenia), University of Iceland (Reykjavik, Iceland); Karlsruhe Institute of Technology (Germany); Kiepenheuer Institute for Solar Physics (Freiburg, Germany); Université Clermont Auvergne (Clermont-Ferrand, France); INRA, PACA Research Center.

- Interdisciplinary networks and centers: “Microbes at the Interface of Land-Atmosphere Feedbacks (MILAF)”; AtmosphericA; Arctic Research Center; Stellar Astrophysics Centre, Strategic Growth Area Arctic (SGA Arctic) ENVS, AU, iCLIMATE Aarhus University Interdisciplinary Centre for Climate Change.

- Co-editor: Frontiers in Microbiology, Research Topic “The Atmospheric Microbiota”.

11. Supervision of students Co-supervision of 6 PhD students and supervision of 9 MSc students, 3 BSc student, 12 project students, from Aarhus University, Lund University, University of Iceland, University of Ljubljana, Bremen University of Applied Sciences.

12. Invited talks - Danish Microbiological Society Congress, 2019, Copenhagen, Denmark - X. Congress of Slovenian Scientists and Economists, 2019, Ljubljana, Slovenia - Leibniz Institute for Tropospheric Research, 2019, Leipzig, Germany - Danish Sea Ice Forum, 2018, Copenhagen, Denmark - Institute for Marine Sciences, 2018, Barcelona, Spain - International Aerosol Conference, 2018, Missouri, US - Strategic Growth Area Arctic Workshop, 2017, Roskilde, Denmark - The Early History of Planetary Systems and Habitable Planets Conference, session “Co-evolution

of Earth's geosphere and biosphere and the evolution of life”, 2017, Tartu, Estonia - Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen,

Denmark - Stellar Astrophysics Centre annual meeting, 2017, Sandbjerg, Denmark - Danish Microbiological Society Congress, 2016, Copenhagen, Denmark - Arctic Research Center annual meeting, 2016, Sandbjerg, Denmark - Max Planck Institute for Chemistry, 2016, Mainz, Germany - Kiepenheuer Institute for Solar Physics (KIS), 2016, Freiburg, Germany - Network meeting of Atmospherica interdisciplinary network, 2016, Sandbjerg, Denmark - Gothenburg atmospheric Science Center, University of Gothenburg, 2016, Gothenburg, Sweden - Microbes at the Interface of Land-Atmosphere Feedbacks Workshop, 2016, Bolzano, Italy - 12th Informal Conference on Atmospheric and Molecular Science, 2015, Aarhus, Denmark - INTIME 9 International meeting on new techniques in microbial ecology, 2015, Nussdorf am

Attersee, Austria - INTIME 8 International meeting on new techniques in microbial ecology, 2013, Ry, Denmark - CRAICC Ice Nucleation Workshop, 2011, Copenhagen, Denmark

13. Outreach activities Oct 2019 A public talk for Six Degrees of Science Mar-Oct 2019 Three radio interviews for Val 2020 (a national Slovene radio broadcasting station) Nov 2018 The Velux Foundations Yearbook 2018 Nov 2018 Interview for a book on Exoplanets Feb 2018 A public talk for Astronomy on Tap, Aarhus, Denmark Oct 2017 ExoLife: Live Hangouts with Deep Astronomy

www.youtube.com/playlist?list= PLqUxw0yinjbpfEG1u4DhL14YXQx8inKam

Oct 2017 Activity for Girls in Science, Biology week, Aarhus University Sep 2017 A live online chat on extreme lifeforms, crowdfunding for ExoLife Finder (ELF)

Telescope, organized by Prof. Svetlana Berdyugina Mar 2017 A lecture for Astronomidagen, Aarhus University Dec 2015 Two lectures and “Dinner with an astronomer” activity at the ESO Astronomy Camp

(The Solar System and the Extrasolar Planets) for secondary school children, Astronomical Observatory of the Autonomous Region of the Aosta Valley

Dec 2015 Participated in an interview “Winter flu is creating major problems” for the local Swedish television SVT Skåne

Aug 2015 Interview for the article Brix L (2015). Ny metode kan finde liv i rummet på store afstande. Videnskab dk. http://videnskab.dk/teknologi/ny-metode-kan-finde-liv-i-rummet-pa-store-afstande

Aug 2015 Interview for the articles - Brix L (2015). Bakterier skal forbedre fremtidens vejrudsigter. Videnskab dk.

http://videnskab.dk/miljo-naturvidenskab/bakterier-skal-forbedre-fremtidens-vejrudsigter

- Brix L (2015). Bacteria in the atmosphere cause rain. Science Nordic. http://sciencenordic.com/bacteria-atmosphere-cause-rain

Jan 2013 Interviews for the articles - Gozlan PM (2013). Les microbes, peuple des nuages. Le Monde.

http://www.lemonde.fr/sciences/article/2013/01/24/les-microbes-peuple-des-nuages_1822292_1650684.html

- Blawat VK (2013). Leben in den Wolken. Süd-deutsche Zeitung. http://www.sueddeutsche.de/wissen/bakterien-leben-in-den-wolken-1.1581747

- Hildebrandt S (2013). Tordenskyer bugner af levende bakterier. Videnskab dk. http://videnskab.dk/miljo-naturvidenskab/tordenskyer-bugner-af-levende-bakterier

2013 Involved in writing articles: - Finster K, Šantl-Temkiv T, Kjeldsen H (2013). Er der liv derude? Aktuel

Naturvidenskab 6: 22–25. - Finster K, Šantl-Temkiv T, Karlson UG (2013). Vejrudsigten: nu med bakterier.

Aktuel Naturvidenskab 10: 10–15.