Sampling For Success
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Sample collection & submission, chain of custody, choosing the right test for Microbiological testing
description
Sample collection & submission, chain of custody, choosing the right test for Microbiological testing. Sampling For Success. Common Types of Microbiological Samples for Food Plants. Swabs (stick, sponge, cloth) Bulk – Raw materials, Finished Products Water. - PowerPoint PPT Presentation
Transcript of Sampling For Success
Sample collection & submission, chain of custody, choosing the right test
for Microbiological testing
Common Types of Microbiological Samples for Food Plants
Swabs (stick, sponge, cloth)
Bulk – Raw materials, Finished Products
Water
Preparing to Collect Swab Samples Appropriate type and amount of swabs Labelling materials Sanitizer Neutralizing buffer Chain of custody Sampling plan
Types of Swabs
Stick
Sponge
Cloth
Swabbing Technique Label swab, noting
sampling location Open swab
aseptically and swab sample site
Close swab and sanitize swab site
Refrigerate swabs until transport to laboratory
Preparing to Collect Product Samples Sterile Sampling Materials
Spoons, forceps, knivesSample bags or containersGloves, other protective apparel as required
Labelling Materials Chain of Custody Sampling Plan
Product Sampling Guidelines Collect unopened containers if possible If collecting from bulk containers, ensure
sample is representative of the product being sampled
Make sure to collect at least enough sample to meet analysis requirements
Store samples at appropriate temperature in preparation for delivery to the laboratory
Planning for Collecting a Water Sample:
Sterile Sample Bottlespreservative
Labels Chain of Custody Cooler and Ice Packs Transportation Arrangements
Water Sample Site ConsiderationsSites to Avoid: Dead end mains Seldom used faucets Faucets with a point of use filter eg.
Carbon Garden or other flexible hoses
Collecting the Water Sample Pre-label the sample container -Date, time of sample, site location Remove any aerators or screens from
the faucet Run the faucet cold for at least 2
minutes Open the sample bottle and collect the
water until the shoulder, leaving a headspace
Seal the sampling container tightly
Preparing Samples for Shipping to the Laboratory Place samples in a cooler with an ice
pack Do not allow water samples to freeze Avoid loose ice Include Complete Chain of Custody
(COC) Label cooler clearly with site location
and destination laboratory location
Important Components of a Chain of Custody (COC) Identifies sender and receiver of
sampled materials Specifies analyses and methodologies
to be performed on samples Provides descriptions of samples for
reporting of analysis results Serves as a legal document for the
traceability of the handling and possession of samples
Points to Remember when Completing COCs Contact information for company and
sampler is available Sample descriptions allow for the easy
identification of the samples included Analysis and methodology indicated Sampling plan requirements clearly
stated
Chain of Custody (Water)
Client Information
Sample Site Identifications
Name of Sampler
Date and time of sample collections
Analysis Requested
Drinking/ Non-drinking water
Choosing the Right Test
Consider the Objectives of Testing:Sanitation monitoring
○ Standard Plate Count, Coliforms, Yeast & Mold
Regulatory compliance○ Correct method for compliance
Lab accreditation for methodology
Pathogen screening○ Appropriate for the product/environment
Importance of Proper Sampling
From J. Moldenauer and Sutton, “Towards an Improved Sterility Test,” Commentary, PDA Journal of Pharmaceutical Science and Technology
Odds of catching sterility breakdown in a batch when taking 20 samples for USP sterility test
