Room Temperature Stable PCR and qPCR Reagents€¦ · Leading stable PCR reagent supplier Trusted...
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Room Temperature Stable PCR and qPCR Reagents NEW! SolisFAST ® product range solisbiodyne.com SOLIS BIODYNE OÜ E: [email protected] T: +372 7409 960 F: +372 7402 079
Transcript of Room Temperature Stable PCR and qPCR Reagents€¦ · Leading stable PCR reagent supplier Trusted...
Room Temperature Stable PCR and qPCR ReagentsNEW!SolisFAST®
product range
solisbiodyne.com
SOLIS BIODYNE OÜE: [email protected]: +372 7409 960F: +372 7402 079
Stability TAG technology
About us
Evolution of Product Portfolio
We offer•High quality stable PCR, qPCR
and reverse transcription reagents
•Great lot-to-lot consistency•Custom-made solutions
•Personalized technical support•Fast service and delivery•Best value for money•FREE samples
For more information visit solisbiodyne.com
Established in 1995
Leading stable PCR reagent supplier
Trusted trademark in 110+ countries
55+ catalogue products
60% revenue from diagnostic sector
Patents in EU, US and South Korea
ISO 9001 and ISO 13485 certified
All our enzymes are genetically modified with a „Stability TAG“ – this ensures the enzymes are fully active even after staying at room temperature for 30 days! All our reagents are suitable for room temperature reaction set-up and are shipped without ice.
No need to pay for shipping the ice
DRY ICEIN REGULAR
INSULATED BOX
ACTUALPRODUCT SIZE
Comparison of our 5x mix to a standard 2x mix
Vial size Reactions (20 µl finalvolume)
2x Standard Mix 1 ml 100 rxn
5x Solis BioDyne Mix 1 ml 250 rxn
Our(q)PCRMasterMixesare 5x concentrated
5x advantages (over 2x mixes)• More reactions from the same volume• More space for low-concentrated sample• More space for primers and probes• Better stability•Best fit for multiplex reactions
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2015
2016
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2018
Established
FIREPol® and Master Mixes
HOT FIREPol® and Master Mixes
qPCR Mixes EvaGreen® and Probe
Probe Universal qPCR Mix
Multiplex PCR Master Mix
SolisGreen® qPCR Mix
2019
2020
SolisFAST® product range
One-step SolisGreen® RT-qPCR
cDNA synthesis Kits and MixesFIREScript® and SOLIScript®
One-step probe-based RT-qPCRMultiplex qPCR Master Mix
Blend PCR Master Mix
2011 EvaGreen® qPCR Supermix
Key products
Dye-based qPCR
Endpoint PCR
Probe-based qPCR
Solis BioDyne Supermix
Comp. A Comp. B Comp. C
HOT FIREPol® EvaGreen® qPCR Supermix•Stable, safe and sensitive EvaGreen® dye•Suitable for GC-rich targets•Contains blue visualisation dye•Contains dUTP for UNG
decontamination protocols
HOT FIREPol®BlendMasterMix•Contains proofreading enzyme •Up to 5xhigherfidelity
compared to Taq alone•Higher yield, specifi city and sensitivity
HOT FIREPol® Probe UniversalqPCRMix•Excellent 2-plex performance•Suitable for GC-rich targets•Suitable for all cyclers•Contains dUTP for UNG
decontamination protocols
M 1 2 3 4 5 6
Lane123456
Templateλ DNA λ DNA λ DNA λ DNA Mouse genomic DNA Mouse genomic DNA
Amplicon Length499 bp
1003 bp1998 bp4991 bp
808 bp3838 bp
12 14 16 18 20 22 24 26 28 30 32 34 36 38 4 0 42 44
1E00
0.01
Amplification Plot
ΔRn
CYCLE
GADPH-VIC BAIP3-FAM
1E05
1E04
ΔRn
Cycle20 22 24 26 28 30 32 34 36 38 40
Solis BioDyne Supermix Comp. A Comp. B Comp. C
First-strand cDNA synthesis
FIREScript®cDNAsynthesisMix•For robust two-step RT-PCR and
RT-qPCR •Fast 15 min cDNA synthesis•Wide application range 37-60°C•Suitable for complex templates
Solis BioDyne FIREScript
Comp. A Comp. S3
1-step probe-based RT-qPCR
SOLIScript®1-stepMultiplexProbeKit• cDNA synthesis and probe-based
qPCR in one tube•Excellent performance with
up to 4 targets•Optional ROX or Purple passive
reference dye•Superior specificity FAM ABYJUN VIC
For further details and ordering please [email protected] or call +372 740 9960
New releases
SolisFAST® product range
Fast endpoint PCR
Fast dye-based qPCR
• SolisFAST® Master Mix• Reliable results in single- and
multiplex assays• High specificity and yield• PCR results in less than 30 minutes• dUTP/UNG containing Mix available
SOLIScript® 1-step SolisGreen® Kit• cDNA synthesis and dye-based
qPCR in one tube• High sensitivity and yield• Compatible with most cyclers• Consistent results with low RNA input
SolisFAST® SolisGreen®qPCRMix• Accurate dye-based quantifi cation• Excellent performance with low-
concentrated samples• Optional ROX passive reference dye• 2x faster protocols compared to
standard dye-based qPCR
Fast probe-based qPCR
SolisFAST®ProbeqPCRMix• Accurate probe-based quantifi cation in
up to 5-plex assays• Excellent performance with low-
concentrated samples• Optional ROX and Purple passive
reference dyes• 2x faster protocols compared to
standard probe-based qPCR• dUTP/UNG containing Mix available
FAM Cy5VICABY
M MultiplexSingleplex
RNA input 16 ng 1,6 ng
1-step dye-based RT-qPCR
Available from April 2020
