Products for qPCR and qRT-PCR

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Products for qPCR and qRT-PCR

accelerate > qPCR and qRT-PCR performance

accelerate > qPCR and qRT-PCR performance Improved qPCR specicity and sensitivity Higher eciency over a broad dynamic range Sensitive, specic, and cost-eective detection

High-performance products for qPCR and qRT-PCRHigh-performance research begins with a solid foundation. With Invitrogen and Molecular Probes now under one roof, you can construct your qPCR and qRT-PCR research projects from the ground up with condence. Invitrogens superior Platinum Taq and SuperScript enzymes combined with Molecular Probes detection technologies provide the framework you need to build sensitive, specic qPCR experiments. Our easy-to-use qPCR supermixes, one- and two-step qRT-PCR systems, and uorescent detection platforms are designed and optimized for unsurpassed performance. In addition, our D-LUX uorescent detection platform oers a powerful and convenient alternative to costly probe-based detection methods. No matter what your challenge, youll reach new heights in performance and reliability with solid qPCR and qRT-PCR tools from Invitrogen.

Table of contentsIntroduction ............................................................................................... 3-7 SuperScript III First-Strand Synthesis System for qRT-PCR ........7 qPCR SuperMixes ....................................................................................8-10 Standard ..................................................................................................8-9 SNP genotyping ...................................................................................10 One-Step qRT-PCR Kits ....................................................................... 11-15 One-Step qRT-PCR Kits .................................................................. 11-12 ThermoScriptqRT-PCR Kits .............................................................13 RNA UltraSense qRT-PCR System ..........................................14-15 Two-Step qRT-PCR Kits .......................................................................16-17 qRT-PCR Directly from Cells ..............................................................18-19 For qPCR ........................................................................................... 20-21 For qRT-PCR ............................................................................................ 22 Detection systems ............................................................................. 23-28 Custom D-LUX Fluorogenic Primers................................... 23-26 Certied LUX Fluorogenic Primer Sets ............................. 27-28 qPCR Accessories .................................................................. 19, 26, 29-30 CellsDirect Lysis and Resuspension Buer ................................19 qPCR Instrument Calibration Kit ..................................................... 26 qPCR Plasmid Standards .................................................................... 29 Uracil DNA Glycosylase (UDG) ......................................................... 30 Related Products ........................................................................................ 30

Room Temperature Stable (RTS) Reagents ...............................20-22

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Products for qPCR and qRT-PCR

Comprehensive solutions for gene expression prolingFrom nucleic acid isolation through validation, we have a complete product portfolio to take your gene expression experiments from start to nish.

RNA Purication ChargeSwitch Total RNA Cell Kit PureLink Micro-to-Midi Total RNA Purication Systems TRIzol Reagents

RNA Sample QC Quant-iT RNA Assay Kit RiboGreen RNA Assay Kits

Microarray Labeling and Detection SuperScript cDNA Direct and Indirect Labeling Systems SuperScript RNA Amplication Systems RNAi Knockdown Experiments Validated Stealth RNAi DuoPaks Stealth Select 3 RNAi Sets Custom BLOCK-iT siRNA BLOCK-iT RNAi Vectors

Hybridization Cot-1 DNA Yeast tRNA

qRT-PCR Validation LUX Fluorogenic Primers qPCR SuperMixes One-Step qRT-PCR Kits Two-Step qRT-PCR Kits qPCR Plasmid Standards

For ordering information, please see related products on page 30.

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Unique technologies set us apartOur easy-to-use qPCR and qRT-PCR reagent systems and uorescent detection platforms give you higher sensitivity and greater specicity over a broad dynamic range using the most advanced technologies available: Platinum Taq hot-start technology for improved qPCR specicity and sensitivity SuperScript III Reverse Transcriptase (RT) with increased thermostability and a longer half-life for better sensitivity and the highest cDNA yields Uracil DNA Glycosylase (UDG) for prevention of carryover contamination Room Temperature Stable (RTS) technology for faster, more convenient set-up CellsDirect technology for sensitive qRT-PCR without a separate RNA purication step D-LUX Detection Platform for sensitive, specic, and cost-eective detection

Two-step vs. one-step qRT-PCRTwo-step qRT-PCRTwo-step qRT-PCR is useful for detecting multiple messages from a single RNA sample, performing multiple PCR amplications from a single cDNA sample, and /or preserving cDNA for later applications. Two-step qRT-PCR kits are comprised of separate reverse transcriptase and qPCR modules. In Invitrogens kits, the RT module provides the high-temperature capability of SuperScript III RT and an RT reaction mix specically optimized for rst-strand synthesis of cDNA for use in qPCR. The qPCR module includes Platinum Taq DNA polymerase for increased sensitivity, specicity, and yield.

One-step qRT-PCROne-step qRT-PCR allows easier processing of large numbers of samples from few genes of interest. Both cDNA synthesis and PCR are performed in a single tube using gene-specic primers and RNA target(s) from either RNA or mRNA. All components necessary for qRT-PCR are mixed in the tube, and reverse transcription is directly followed by PCR without additional handling. Reaction tubes are not opened between cDNA synthesis and amplication so carryover contamination is minimized. Furthermore, by amplifying the entire cDNA sample, one-step qRT-PCR enables highly sensitive detection from as few as 10 copies of RNA template, with a broad dynamic range that supports accurate quantication of high-copy mRNA up to 1 g of total RNA. Table 1 compares the benets of one-step and two-step qRT-PCR.

Table 1 Two-step vs. one-step qRT-PCR

Two-step procedure Prime rst-strand DNA with: Provides: Oligo(dT) primer Random hexamers Flexibility Choice of primers for cDNA synthesis Ability to save cDNA for later use Ability to optimize for dicult qRT-PCR Recommended uses: Ideal for detecting or quantifying multiple genes of interest and/or preservation of cDNA

One-step procedure Gene-specic primers

Convenience Amplication enzymes premixed with reverse transcriptase Fewer pipetting steps reduce chances for contamination Well suited for high-throughput applications Ideal for analyzing large numbers of samples from few genes of interest

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Products for qPCR and qRT-PCR

Select the right qPCR product for youqPCR and qRT-PCR product selection guideUse Table 2 to determine the best products for your real-time instrument and application.Table 2 qPCR and qRT-PCR product selection guide SYBR Green Detection Starting materialFormat ABI 7000, 7300, 7700, 7900 instruments Any instrument

Primer/Probe Detection (LUX primers or dual-labeled probes)ABI 7000, 7300, 7700, 7900 instruments Any instrument

Reagents for qPCR and qRT-PCRqPCR SuperMix, Standard, pages 8-9 Platinum SYBR Green qPCR SuperMix-UDG w/ROX Platinum RTS SYBR Green qPCR SuperMix-UDG w/ROX Platinum SYBR Green qPCR SuperMix-UDG Platinum RTS SYBR Green qPCR SuperMix-UDG Platinum qPCR SuperMix-UDG w/ROX Platinum RTS qPCR SuperMix-UDG w/ROX Platinum qPCR SuperMix for Genotyping SuperScript III Platinum Two-Step qRT-PCR Kit w/ROX SuperScript III Platinum CellsDirect Two-Step qRT-PCR Kit SuperScript III Platinum One-Step qRT-PCR Kit w/ROX Platinum qPCR SuperMix-UDG Platinum RTS qPCR SuperMix-UDG Platinum qPCR SuperMix for Genotyping SuperScript III Platinum Two-Step qRT-PCR Kit SuperScript III Platinum CellsDirect Two-Step qRT-PCR Kit SuperScript III Platinum One-Step qRT-PCR Kit

DNA, cDNA

qPCR SuperMix, Room Temperature Stable, pages 20-21 qPCR SuperMix, Genotyping, page 10 Two-Step Kits, Standard, pages 16-17 Two-Step Kits, Directly from cells, pages 18-19 One-Step Kits, Standard, pages 11-12 One-Step Kits, Low Abundance Targets, pages 14-15 One-step Kits, Room Temperature Stable, page 22

N/A SuperScript III Platinum SYBR Green Two-Step qRT-PCR Kit w/ROX SuperScript III Platinum CellsDirect Two-Step qRT-PCR Kit with SYBR Green SuperScript III Platinum SYBR Green One-Step qRT-PCR Kit w/ROX

SuperScript III Platinum SYBR Green Two-Step qRT-PCR Kit SuperScript III Platinum CellsDirect Two-Step qRT-PCR Kit with SYBR Green SuperScript III Platinum SYBR Green One-Step qRT-PCR Kit

RNA

RNA UltraSense One-Step qRT-PCR System SuperScript III Platinum RTS One-Step qRT-PCR Kit w/ROX

RNA UltraSense One-Step qRT-PCR System

SuperScript III Platinum RTS One-Step qRT-PCR Kit

Detection systems for qPCR and qRT-PCRCertied LUX Primer Sets for Human Genes Certied Primer Sets, pages 27-28 N/A Certied LUX Primer Sets for Housekeeping Genes Certied LUX Primer Sets for Infectious Agents Custom Primer Sets, pages 23-26 N/A D-LUX Primer Design Software EvoQuest Custom LUX Primer Design Service

DNA, cDNA, RNA

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Quality enzymes make all the dierencePlatinum Taq hot-start technology improves qPCR sensitivity and specicityWith