RhoGTPases and Actin Cytoskeleton Alex André Andres Elena Isabelle Shuoshuo Structural & Functional...
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Transcript of RhoGTPases and Actin Cytoskeleton Alex André Andres Elena Isabelle Shuoshuo Structural & Functional...
RhoGTPases and Actin Cytoskeleton
Alex
André
Andres
Elena
Isabelle
Shuoshuo
Structural & FunctionalPredictions for
ELMO homologues A Bioinformatic Approach
Overview
Actin cytoskeleton
Cytoskeletal dynamics in cellular processes
Rho GTPases play role in cytoskeletal remodeling
GEFs and GAPs regulate Rho GTPases
ELMO1 – GEF for Rac
ELMO1 (Engulfment and cell motility protein 1)
CED12 in C.elegans phagocytosis of apoptotic cells and cell motility
Not much known (19 papers in Pubmed)
What shall we do?
BIOINFORMATICS
Phylogenetic relationship of ELMO1 homologues
insects
yeasts
vertebratesvertebratesvertebratesvertebratesvertebrates
plants
Nematodes
100100
100
100
64
100
100
Prediction of phosphorylation sites in the ELMO1 sequence
AKT1 Rac-alpha S/T kinase Hck haematopoetic cell kinase
Structure prediction of PH domain by homology remodelling
PH domain identity Geno3D Sequence Identity 23.6%3 models were predicted by homology modelling