rhBMP-2/Calcium Phosphate Matrix Accelerates Osteotomy-Site Healing in a Nonhuman Primate Model at

Multiple Treatment Times and Concentrations

by Howard Seeherman, Rebecca Li, Mary Bouxsein, Hyun Kim, X. Jian Li, Erica A. Smith-Adaline, Maria Aiolova, and John M. Wozney

J Bone Joint Surg AmVolume 88(1):144-160

January 1, 2006

©2006 by The Journal of Bone and Joint Surgery, Inc.

Radiographic appearance of nonhuman primate fibular osteotomy sites treated with 1.5-mg/mL rhBMP-2/calcium phosphate matrix three hours, one day, one week, and two weeks after surgery.

Howard Seeherman et al. J Bone Joint Surg Am 2006;88:144-160

©2006 by The Journal of Bone and Joint Surgery, Inc.

Illustration depicting the retention of I-rhBMP-2, delivered in calcium phosphate matrix (CPM) at one day and one week after surgery, in nonhuman primate fibular osteotomy sites over time (in

weeks).

Howard Seeherman et al. J Bone Joint Surg Am 2006;88:144-160

©2006 by The Journal of Bone and Joint Surgery, Inc.

Histological appearance of callus remodeling at twenty-four months in a nonhuman primate fibular osteotomy site that had been treated with rhBMP-2/calcium phosphate matrix one day

after surgery (A and B, Goldner trichrome; C, unstained section viewed under ...

Howard Seeherman et al. J Bone Joint Surg Am 2006;88:144-160

©2006 by The Journal of Bone and Joint Surgery, Inc.

Radiographs illustrating the appearance, as a function of time after surgery, of the nonhuman primate fibular osteotomy sites that were untreated, that were treated with calcium phosphate

matrix alone (CPM), and that were treated with 0.5, 1.5, and 4.5-mg/m...

Howard Seeherman et al. J Bone Joint Surg Am 2006;88:144-160

©2006 by The Journal of Bone and Joint Surgery, Inc.

Peripheral quantitative computed tomography images, made eight weeks after surgery, showing an osteotomy site that had been treated with 1.5-mg/mL rhBMP-2/calcium phosphate matrix

seven days after surgery and the contralateral, untreated osteotomy site.

Howard Seeherman et al. J Bone Joint Surg Am 2006;88:144-160

©2006 by The Journal of Bone and Joint Surgery, Inc.

Histological appearance of an untreated nonhuman primate fibular osteotomy site (A) at eight weeks after surgery as compared with that of osteotomy sites treated with calcium phosphate

matrix (B) or with 0.5-mg/mL (C), 1.5-mg/mL (D), and 4.5-mg/mL (E) rhBMP...

Howard Seeherman et al. J Bone Joint Surg Am 2006;88:144-160

©2006 by The Journal of Bone and Joint Surgery, Inc.

Histological appearance of an untreated nonhuman primate osteotomy site (A) at three weeks after surgery as compared with that of osteotomy sites treated with rhBMP-2/calcium phosphate

matrix at three hours (B) or seven days (C) after surgery.

Howard Seeherman et al. J Bone Joint Surg Am 2006;88:144-160

©2006 by The Journal of Bone and Joint Surgery, Inc.

A and B: Histological appearance of the cellular infiltrate around an untreated nonhuman primate fibular osteotomy site at three hours after surgery (A) and seven days after surgery (B) (Goldner

trichrome).

Howard Seeherman et al. J Bone Joint Surg Am 2006;88:144-160

©2006 by The Journal of Bone and Joint Surgery, Inc.