Regents Biology Protein Synthesis Making Proteins.
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Transcript of Regents Biology Protein Synthesis Making Proteins.
Regents Biology
Protein Synthesis Making Proteins
Regents Biology
Review of DNA
You have 46 chromosomes.
All those chromosomes are made of DNA.
What is DNA?
Regents Biology
Review of DNA
DNA is a double helix.
Made up of 3 structures. Phosphate Sugar Nitrogen Bases
A, T, C, G
Regents Biology
Review of DNA
DNA IS THE CODE FOR LIFE! DNA is passed on from generation to
generation. MEIOSIS
DNA is copied when our bodies need to heal.
HOW DOES IT DO THIS?
Regents Biology
Bodies are made up of cells All cells run on a set of instructions
spelled out in DNA
Bodies Cells DNA
Regents Biology
How does DNA code for cells & bodies? how are cells and bodies made from the
instructions in DNA
DNA Cells Bodies
Regents Biology
DNA has the information to build proteins genes
DNA Proteins Cells Bodies
proteinscells
bodiesDNA gets all the glory,Proteins do all the work
Regents Biology
How do proteins do all the work Proteins
proteins run living organisms enzymes
control all chemical reactions in living organisms
structure all living organisms are built out of proteins
Regents Biology
cytoplasm
nucleus
Cell organization DNA
DNA is in the nucleus genes = instructions for making proteins
want to keep it there = protected “locked in the vault”
Regents Biology
Cell organization Proteins
chains of amino acids made by a “protein factory” in cytoplasm protein factory = ribosome
nucleus
cytoplasm
ribosome
aa
aa
aa
aa
aa
aa
aa
aaaa
aa
buildproteins
Regents Biology
Passing on DNA information Need to get DNA gene information
from nucleus to cytoplasm need a copy of DNA messenger RNA
nucleus
cytoplasm
ribosome
mRNA
buildproteins
aa
aa
aa
aa
aa
aa
aa
aaaa
aa
Regents Biology
mRNA
From nucleus to cytoplasm
DNA
transcription
nucleus cytoplasm
translation
trait
aa
aa
aa
aa
aa
aa
aa
aaaa
aa
protein
Regents Biology
DNA vs. RNADNA
deoxyribose sugar nitrogen bases
G, C, A, T T : A C : G
double stranded
RNA ribose sugar nitrogen bases
G, C, A, U U : A C : G
single stranded
Regents Biology
Transcription Transcription - Making mRNA from DNA DNA strand is the
template (pattern) match bases
U : A G : C
Enzyme RNA polymerase
Regents Biology
Matching bases of DNA & RNA Double stranded DNA unzips
A G GGGGGT T A C A C T T T T TC C C CA A
Regents Biology
Matching bases of DNA & RNA Double stranded DNA unzips
A G GGGGGT T A C A C T T T T TC C C CA A
Regents Biology
Matching bases of DNA & RNA Match RNA bases to DNA
bases on one of the DNA strands
U
A G GGGGGT T A C A C T T T T TC C C CA A
U
UU
U
U
G
G
A
A
A C CRNA
polymerase
C
C
C
C
C
G
G
G
G
A
A
A
AA
Regents Biology
Matching bases of DNA & RNA U instead of T is matched to A
TACGCACATTTACGTACGCGGDNA
AUGCGUGUAAAUGCAUGCGCCmRNAaa
aa
aa
aa
aa
aa
aa
aaaa
aa
U C CCCCCA A U G U G A A A A AG G G GU Uribosome
Regents Biology
aa
aa
aa
aa
aa
aa
aa
aaaa
aa
protein
cytoplasm
nucleus
traitU C CCCCCA A U G U G A A A A AG G G GU U
ribosome
Because the RNA leaves to go outside the nucleus, we call it mRNA, or MESSENGER RNA
Regents Biology
How does mRNA code for proteins mRNA leaves nucleus mRNA goes to ribosomes in cytoplasm Proteins built from instructions on mRNA
aa aa aa aa aa aa aa aa
How?
mRNA
U C CCCCCA A U G U G A A A A AG G G GU U
Regents Biology
How does mRNA code for proteins?
TACGCACATTTACGTACGCGGDNA
AUGCGUGUAAAUGCAUGCGCCmRNA
Met Arg Val Asn Ala Cys Alaprotein
?
How can you code for 20 amino acids withonly 4 DNA bases (A,U,G,C)?
ribosome
aa aa aa aa aa aa aa aa
Regents Biology
AUGCGUGUAAAUGCAUGCGCCmRNA
mRNA codes for proteins in triplets
TACGCACATTTACGTACGCGGDNA
AUGCGUGUAAAUGCAUGCGCCmRNA
Met Arg Val Asn Ala Cys Alaprotein
?
Codon = block of 3 mRNA bases
codon
ribosome
Regents Biology
There are only 20 amino acids
These amino acids code for ALL life!
strongest support for a common origin for all life
Code has duplicates several codons for
each amino acid mutation insurance!
The mRNA code
amino acid
protein –
made of 4 amino acids
Regents Biology
Start codon – code that starts the protein making
AUG methionine
Stop codons – code that ends protein making
UGA, UAA, UAG
The mRNA code
Regents Biology
How are the codons matched to amino acids?
Transfer RNA, or tRNA – brings the amino acid to the ribosome
On the amino acid is an anticodon Anti-codon – block of 3 tRNA bases
Regents Biology
How are the codons matched to amino acids?
TACGCACATTTACGTACGCGGDNA
AUGCGUGUAAAUGCAUGCGCCmRNA
anti-codon
codon
tRNAUAC
MetGCA
ArgCAU
Val Anti-codon = block of 3 tRNA bases
aminoacid
Regents Biology
Translation Translation – when mRNA makes the protein
The working instructions mRNA The reader ribosome The transporter transfer RNA (tRNA)
mRNAU C CCCCCA A U G U G A A A A AG G G GU U
aaaa
aa
tRNA
GGU
aa
tRNA
U A C
aa
tRNA
GA C
tRNA
aa
A GU
ribosome
Regents Biology
aa
aa
aaaa
aa
aa
aa
mRNA
From gene to protein
DNA
transcription
nucleus cytoplasm
protein
translation
trait
U C CCCCCA A U G U G A A A A AG G G GU Uribosome
tRNA
aa
Regents Biology
protein
aa
aa
aa
aa
aa
aa
aa
aaaa
aa
aa
transcription
cytoplasm
nucleus
translation
trait
Regents Biology
From gene to protein
transcriptiontranscription
translationtranslation
proteinprotein
Regents Biology
http://www.wisc-online.com/Objects/ViewObject.aspx?ID=AP1302
Interactive
Regents Biology
http://www.learnerstv.com/animation/biology/Proteinsynthesis.swf
Interactive
Regents Biology 2009-2010
Whoops!See what happens when
your genes don’t work right!
Any Questions??