Synthesis and targetting of mitochondrial proteins
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Transcript of Synthesis and targetting of mitochondrial proteins
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SYNTHESIS AND TARGETTING OF
MITOCHINDRIAL PROTEINS
PREPARD BY:
AJAY BADYAL
M.SC. ZOOLOGY
SUBMITTED TO
DEPARTMENT OF ZOOLOGY AND APPLIED AQUACULTURE B.U
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SYNPOSIS • INTRODUCTION
• MITOCHONDRIAL PROTEINS.
• PROTEIN TARGETTING IN SORTING.
• SYNTHESIS OF MITOCHONDRIAL PROTEINS.
• CONCLUSION
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INTRODUCTION
• Mitochondria are consider as the power house of the cell because in it the energy is liberated and released in the form of ATP which is generated thorough the ADP+Pi.
• It contains their own DNA. Most of the proteins of this organelle are imported to the cytosol after their synthesis. Most of the proteins are synthesized outside the cytosolic ribosome.
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• Newly formed proteins are released into the cytosol. The receptors recognise specific uptake targeting sequences in the new proteins.
• Targeting of sub proteins acquires sequential action of two targeting sequences and two membrane bound receptor. One is to direct the protein into the mitochondria & the other to direct the correct organelle or membrane.
• Generally the protein uptake into the mitochondria is an energy requiring process that depends upon the Mito. Membranes.
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List of some mitochondrial proteins
Location of proteins in mitochondria Name of the proteins
MATRIX 1. ALCHOL DEHYDROGENASE (YEAST)
2. CARBOXYLIC PHOSPHATE SYNTHETASE
3. CITRATE SYNTHETASE & OTHER CITRIC ACID ENZYMES
4. F1 ATPase SUBUNIT , α,β, & γ
5. ORINTHINE METHYL TRANSFERASE
ORITHININE TRANS CARBOXYLASE
INNER MEMBRANE ADP/ATP ANTIPORTER
CYT.Q CoQH2 (C – REDUCTASE COMPLEX)
SUB UNIT 1,2,5,6,7& 8 Cyt. C OXIDASE SUBUNIT
PHOSPHATE/OH ANTIPORTER
PROTEYOLYTIC OF F0 ATPase
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INTER MEMBRANE SPACE Cyt. C
Cyt. C PEROXIDASE
Cyt. B2 & C1 (SUBUNIT OF CQH2- Cyt- REDUCTASE COMPLEX)
OUTER MEMBRANE MITOCHONDRIAL PORINS (H2O TRANSPORT ) P70
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SYHTHESIS OF
MITOCHINDRIAL PROTEINS
• Most of the proteins of mitochondria are synthesised in the cytosol.
• All newly formed mitochondrial proteins are formed are initially located in the cytosol.
• Travelling proteins have their each n-terminus residues comprises most targeting sequences.
• The receptor signal sequences are not identical receptor itself recognises such sequences at different destination from mitochondria now are ale to bind through a different but related sequences.
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Site of synthesis
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PROTEIN TARGETTING IN
SORTING • Mainly most of the proteins of the cytosol or in the
mitochondria are targeting by the two chaperons mitochondrial import stimulation factor (MSF)i.e. HSP-70 and HSP-60.
• The sites from where it goes in side the matrix of the mitochondria is two types TOM-22 & TOM-23 they get further binds up with TOM-70 & TOM-37.
• Targeting while in sorting of proteins is done by the two processes these are CO-translational import and post translational import it occurs at the site where chaperons proteins act n brings proximity an d made a PMF across the membrane.
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CONCLUSION • FROM THE ABOVE DISCCUSSION WE
KNOW ABOUT THE MITOCHONDRIAL PROTEINS THEIR SYNTHEISI I N DIFFERENT SITES INSIDE THE ORGANELLE THEN WE KNOW ABOUT THE WAY HOW THEY TARGETTING AT THEIR DESIRED LOCATION/SITES IN THE MITOCHONDRIA OR CYTOSOL AND HOW THEY GENERATES THE ENERGY I NTHE FORM OF PHOSPHATS.
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THANKS FOR YOUR ATTENTION…
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