TNAU PDB- Tamil Nadu Agricultural University proteome database- Horse gram proteome
Proteome Analysis July 1, 2009 Learning objectives. Understand the theoretical basis of 2D-SDS Gel...
-
date post
22-Dec-2015 -
Category
Documents
-
view
217 -
download
4
Transcript of Proteome Analysis July 1, 2009 Learning objectives. Understand the theoretical basis of 2D-SDS Gel...
Proteome Analysis
July 1, 2009
Learning objectives. Understand the theoretical basis of 2D-SDS Gel Electrophoresis. Understand the latest technique for identifying proteins using mass spectrometry. Become familiar with the SWISS-2DPAGE Database
Workshop on identifying proteins with the pI, MW and a few peptide masses.
2D-SDS Gel ElectrophoresisWhat is it?
This is a method for the separation and identification of proteins in a sample by displacement in 2 dimensions oriented at right angles to one another.
Proteins separate over a large area, having a greater chance of resolution.
First dimension
Separation by isoelectric point
Isoelectric point-pH at which the net charge on the molecule is zero
Visuals
http://www.aber.ac.uk/parasitology/Proteome/Tut_2D.html#Section%203
2nd Dimension-to separate by size
Five steps:1. Isolate proteins
2. Denature proteins
3. Load onto gel
4. Apply voltage gradient
5. Stain
Databases of 2D-Gels
http://www.umh.ac.be/~biochim/balhf.htm
http://www-lmmb.ncifcrf.gov/EP/table2Ddatabases.html
http://www.weihenstephan.de/blm/deg/manual/manualwork2html02testp9.htm