Shubash Chandra DASGraduate School of Biosphere Science

Hiroshima University

Prolonged sperm survivability in hen oviduct

鶏卵管における精子の生存の延長

Who I am and where I came from?

Bangladesh and Japan:basic cultural differences

What is my research?

Today I will talk

Practical demonstration!!

Name : Shubash Chandra Das

Who I am and where I came from?

Position:(i) In Japan: JSPS post-doctoral research fellow

Hiroshima UniversityHigashi-Hiroshima Campus

(ii) In Bangladesh: Associate ProfessorBangladesh Agricultural University, Mymensingh, Bangladesh

Bangladesh

Japa

n

Map of the World

Latin America

Africa

Oceania

Asia

EuropeNorth America

Bangladesh

Japan

Map of the World

日本

バングラデシュ

Bay of Bengal

Map of South Asia

バングラデシュ

Sylhet4.2

Dhaka2.9

Rajshahi2.6

Khulna 2.8

Barisal2.9

Chittagong3.7

Bangladesh Agrl. University, Mymensingh(My job place)

Map of Bangladesh

Naogaon(Where I born)生誕地

職場

My name:Shubash

Bangladeshi people:name styles!

Subash Bose:LEADER

Wife’s name:Bijoya

Kid’s name:Boijayanti

Voijayanti Mala:Indian actressBijoya:

Hindu GOD

Bangladesh and Japan: basic cultural differencesバングラデシュと日本：基本的な文化の違い

インドの女優

ヒンドゥー教の神様

Apple story(Sir Isaac Newton)

Japanese people: name styles!

Swimming in the river(President Barack Obama)

木下さん 川中さん

Name of human being:meaning varies in different cultures!人の名前の意味は文化によって違う

Bangladesh Japan

Related to GOD and famous persons神様と有名人に関係している

Related to natural matters

自然にも関係している

What is other differences?そのほかに違いはあるか？

Jackfruit Melon

Bangladeshi fruit Japanese fruit

ジャックフルーツ

Populations: 140 128 (millions)

Pop. density: 1045人 340人(per sq. km)

Growth rate : 1.3 -0.088(%)

Bangladesh Japan

Over-populations人口過密

(1.3億人)(1.4億人)

Japan

Why over-populations?なぜ人口が多いのか？

1 2

34

Bangladesh

I have 8wives

Mating in chicken

Mating ratio=Male: Female

1 : 8 Why?

What is my research?

For good egg fertility: (85-90%)

自然交配

オス

メス

We keep male and female at the ratio of 1 : 8 for natural mating and we get fertile eggs.

Fertile egg Chick

How egg formation and fertilization occur?

21 days incubation

オス:メス＝1:8で飼育して、受精卵を得る

卵はどのようにしてでき、受精はどのようにして起こるか？

Formation of eggs and fertilization

Egg formation

Fertilization

Sperm is essential for fertilization

24 h

rs

Germinal disc卵巣

漏斗部

膨大部

峡部

子宮部

膣部

受精のために精子が必要

胚盤受精

How and from where the sperm will come to the site of fertilization?

After natural mating or AI, sperm are stored and survive in the UVJ of oviduct.

Hen oviduct

卵巣

漏斗部

膨大部

峡部

子宮部

膣部

精子はどのようにどこから来るのか？

人工授精後、精子は子宮膣移行部に溜まり、生存する

Sperm storage in the female reproductive tract

Mammals

Human

Cow

Boar

1d

2d

2-3d

Birds

Quail

Chicken

Turkey

2 wks

3 wks

6 wks

Birds can storage sperm for prolonged period

メスの生殖器における精子生存

鳥類は卵管で精子を長期間生存させることができる

How the laying hen can store sperm in their oviduct for prolonged period?

Ovary

Infund Mag

Isth

UtVag

UVJSperm storage tubules (SST)

in the section of UVJ

SST

Sperm

精子は鳥類の卵管でどのように長生きするのか？

精子は精子貯蔵細管で生存する

Because of the sperm storage, we get few no. of fertile eggs after single dose of AI

AI: 2/10

Fertile egg2/12

Fertile egg2/13

Fertile egg2/14

Fertile egg2/15

Fertile egg2/16

Fertile egg2/17

Fertile egg2/187-10 fertile eggs after 1 AI

1回の受精で7～10個の受精卵が得られる

Mechanism of sperm storage in the SST?

Sperm

Lymphocytes

Local immunity of UVJ should be suppressed

SSTにおける精子生存のメカニズム

精子

リンパ球

精子生存のためにUVJの免疫が抑制される

1. We found the higher expressions of transforming growth factor-βs (TGF-βs) in the UVJ after AI.

2. TGF-βs has immunosuppressive functions. 3. Thus, TGF-βs may suppressive immunity of UVJ, and

sperm can survive.

TGFβ

Anti-sperm immunoresponse?

Immunosupression?

Local immunity of UVJ is suppressed by TGF-βs

UVJの免疫はTGF-βによって抑制される

精子はニワトリ卵管で長期間生存する

精子はSSTという特別な貯蔵細管に貯蔵される

TGF-βは精子の生存のためUVJの免疫を抑制す

る

1回の受精で7～10個の受精卵が得られる