Program - UMIN

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Program November, 23th, Thursday 12：15 ～ 13：00 Satellite Session Room D (Sub Exhibition Hall: 2F)

Chair: Daisaku Koyama (Lush Japan Co. Ltd) SS-1 The biggest prize in the world “Lush Prize” and newest world trend of the animal testing alternatives.

Rebecca Ram Scientific Research Consultant, Lush Prize UK SS-2 The challenge to establish individual liver assay models and social system for human specimen

utilization Kumiko Tatsumi1),2)

1) Department of Gastrointestinal Surgery, Graduate School of Medicine, Kyoto University, Japan

2) Department of Biochemistry, Osaka International Cancer Institute, Japan

Supported by Lush Prize 13：15～13：30 Opening ceremony Room A+B ( Convention Hall : 4F)

13：30～14：15 Keynote Lecture Room A+B ( Convention Hall : 4F)

Chair: Makoto Hayashi (makoto international consulting：mic） Regulatory Science for realizing 3Rs in animal tests Toru Kawanishi

National Institute of Health Sciences, Japan

14：15～15：00 Invited Lecture Room A+B ( Convention Hall :4F)

Chair: Noriho Tanaka (Food and Drug Safety Center, Hatano Research Institute) Validation and international acceptance of alternative methods: New ways forward

Chantra Eskes SeCAM, Switzerland 15：30 ～ 18：00 Symposium 1 Room A+B ( Convention Hall : 4F)

Co-chair：Yasuaki Sakai University of Tokyo)Toshiyuki Kanamori (National Institute of Advanced Industrial Science and Technology: AIST)

Organs-on-a chip as promising alternatives to animal experiments S1-1 Expectations and infrastructure improvement for Organs-on-a-chip as drug discovery tool

Seiichi Ishida

Division of Pharmacology, National Institute of Health Sciences, Japan

S1-2 Organs-on-a-chip: anticipation of use for alternatives to animal experiments in chemical companies

Koichi Saito

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Sumitomo Chemical Co., Ltd. Advanced Materials Development Laboratory, Japan

S1-3 Technical challenges to industrialization of organs-on-a-chip

Shinji Sugiura and Toshiyuki Kanamori

National Institute of Advanced Industrial Science and Technology (AIST), Japan

S1-4 Organs-on-a-chip: biological problems to be solved

Naohiko Anzai1)2), Motoshi Ouchi2) and Tomomi Furihata1)

1) Chiba University Graduate School of Medicine, Japan

2) Dokkyo Medical University School of Medicine, Japan Supported by the Society for Cell Assay

15:30～17：30 Symposium 2 Room C ( Conference Room : 3F)

Co-Chair：Kotaro Yuta (In Silico Data, Ltd.) Yumi Akahori (Chemicals Assessment and Research Center)

Basic and application of "Read-across", present and future development of compounds regulation S2-1 Read-across within Regulatory Context

Yumi Akahori

Chemicals Assessment and Research Center, Chemicals Evaluation and Research Institute, Japan

S2-2 In silico computer study and toxicology testing

Yoshihisa Inoue

IncromCRO, Japan

S2-3 In silico prediction of the major clearance pathways of drugs and substrates of drug transporters

based on the chemical structure of drugs

Kazuya Maeda

Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan

Supported by Chem-Bio Informatics Society(CBI) S2-4 Kazukuni Fukuhara Ministry of Economy, Trade and Industry, Japan 15:30～18：00 Symposium 3 Room D (Sub Exhibition Hall: 2F)

Co-chair：Takashi Agui (Hokkaido University Veterinary School) Tsutomu Miki Kurosawa (Kagoshima University Veterinary School)

Current AAALAC International accreditation；Present and future S3-1 AAALAC International Update: Present and Future

James R. Swearengen,

AAALAC International

S3-2 AAALAC International Accreditation Program in Korea: Present and Future

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Byung-in Choe1), Gwi Hyang Lee 1,2), Yechan Jung3)

1) Nicholas Cardinal Cheong Graduate School for Life, The Catholic University of Korea, Seoul,

Korea

2) Bioethics Information Center, Seoul, Korea,

3) Research Ethics Center, Korea University, Seoul, Korea

S3-3 What I've learned through the experience of AAALAC's accreditation

Nobuya Sasaki, DVM, PhD, DJCLAM

Laboratory of Laboratory Animal Science. Kitasato University, Japan

S3-4 AAALAC International from the standpoint of a Japanese pharmaceutical company

Hidenori Watanabe

JAPAN TOBACCO INC. Central Pharmaceutical Research Institute, Japan

Supported by the Japanese Association for Laboratory Animal

Medicine (JALAM)& JSAAE International committee

November, 24th, Friday 9:30～11:20 Short presentation P-01～P-22 Room A ( Convention Hall : 4F) P-23～P-44 Room B( Convention Hall : 4F) P-45～P-66 Room C ( Conference Room : 3F) P-67～P-88 Room D (Sub Exhibition Hall: 2F) 11:30～12：00 Mandom Session Room D (Sub Exhibition Hall: 2F)

Chair：Tatsuya Watanage (Mandom Cooperation)

M-0 Welcome address Mandaom international foundation for alternative to animal tesitng Tatsuya Watanage Mandom Cooperation, Japan M-1 Development of an in vitro evaluation system for drugs with liver injury potentially associated with

mitochondrial toxicity

Kousei Ito

Graduate School of Pharmaceutical Sciences, Chiba University, Japan

M-2 Protective effects and safety of molecular hydrogen on H9c2 cardiomyocytes model

Yuji Yoshiyama

Research and education center for clinical pharmacy, School of pharmacy, Kitasato University, Japan

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12:10～13：00 Luncheon Seminar 1 Room A+B ( Convention Hall : 4F) Chair: Hirohiko Tsuzuki (Cellular Dynamics International, Inc)

LS1-1 Introduction of alternative animal testing method for prediction of eye irritation

Masakazu Katoh

Japan Tissue Engineering Co., Ltd., Japan

LS1-2 In vitro screening of compounds with severe drug-induced liver injury risk focusing on differentiation

and maturation process of hepatocytes

Kousei Ito

Graduate School of Pharmaceutical Sciences, Chiba University, Japan

Supported by Japan Tissue Engineering Co., Ltd. & Cellular Dynamics International, Inc

13:10～14：30 General meeting and JSAAE Award Ceremony Room D (Sub Exhibition Hall: 2F)

14:30～15:00 JSAAE AWARD Room D (Sub Exhibition Hall: 2F)

Chair: Yasuaki Sakai University of Tokyo)

The development of the IL-8 Luc assay (OECD TG 442E) Setsuya Aiba

Department of Dermatology, Tohoku University Graduate School of Medicine, Japan

15:15～17:15 Poster Discussion Main Exhibition Hall (1F)

Odd number 15：15～16：15 Even number 16：15～17：15 17:30～18:15 Special Lecture Room D (Sub Exhibition Hall: 2F)

Chair：Yasuo Ohno (Kihara Memorial Yokohama Foundation)

Trends of 3Rs in OECD and ICH Akiyoshi Nishikawa

Biological Safety Research Center, National Institute of Health Sciences, Japan

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November, 25th, Saturday 9:30～10:15 Educational Lecture Room D (Sub Exhibition Hall: 2F)

Chair: Yasuaki Sakai (University of Tokyo)

The dilemma of animal welfare and the law concerning the welfare and control of animals Masashi Norihisa

Animal Welfare and Management Office, Nature Conservation Bureau, Ministry of the Environment

10:30～12:30 Panel Discussion Room D (Sub Exhibition Hall: 2F)

Chair：Hiromi Kamekura (Japan Anti-Vivisection Association) One R works. Replacement alternative method to animal testing PD-1 Feeling ahead of "poor" to experimental animals

Kyoko Ota

Japan Children's Literary Association, Japan

PD-2 Significant advance of alternatives, legal reform expected for more progress

Eiko Mori

JIJI PRESS, Japan

PD-3 Outline of regulations on cosmetics and quasi-drugs in Japan

Taku Oohara

Ministry of Health, Labour and Welfare, Japan

PD-4 Kazukuni Fukuhara

Ministry of Economy, Trade and Industry, Japan

PD-5 Alternatives to Animal Testing and Japan’s Role in Animal Welfare Sachiko Azuma PEACE, Tokyo, Japan

PD-6 Sciences toward Animal-Free Understanding of Human Body Responses Yasuyuki Sakai1, 2)

1) Department of Chemical System Engineering, Graduate School of Engineering, University of Tokyo, Japan 2) International Research Center on Integrative Biomedical Systems, Institute of Industrial Science, University of Tokyo, Japan

Supported by the Japan Anti-Vivisection Association

10:30～12:30 Symposium 4 Room A ( Convention Hall : 4F) Co-chair：Yoshiaki Ikarashi ((Division of Environmental Chemistry, National Institute of Health Sciences)

Mariko Sugiyama (Shiseido Company)

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Approach for acceptance of alternatives to animal testing for skin sensitization in approval application of quasi-drugs S4-1 International situation of skin sensitization evaluation

Takao Ashikaga

Division of Risk Assessment, National Institute of Health Sciences, Japan

S4-2 Three alternative test methods in the guidance of a system for evaluating skin sensitization for

quasi-drug applications in Japan

Noriyasu Imai1)2)

1) Japan Cosmetic Industry Association, Tokyo, Japan

2) KOSE Corporation, Tokyo, Japan

S4-3 Guidance for skin sensitization evaluation – 3 out of 3 for bottom-up -

Masaaki Miyazawa1)2)

1) Japan Cosmetic Industry Association, Japan

2) Kao Corporation, Japan

S4-4 The Association of Skin Safety Case Information Network (SSCI-Net); new system with website

registration

Akiko Yagami1)2), Kayoko Suzuki1)2), Kayoko Matsunaga1)3)

1) The Association of SSCI-Net, Japan

2) Department of Allergology, Fujita Health University Second Educational Hospital, Japan

3) Department of Integrative Medical Science for Allergic Disease, Fujita Health University School of

Medicine, Japan

10:30～12:30 Symposium 5 Room B ( Convention Hall : 4F)

Co-chair：Mutsumi Suzuki (Kyowa Hakko Kirin Co., Ltd.) Yoh ichi Tagawa (Tokyo Institute of Technology)

Is Japan Ready? Personalized assay models with social system to guide precision medicine S5-1 Human micro-living system focusing on the tissue structures

Yoh ichi Tagawa

Tokyo Institute of Technology School of Life Science and Technology, Japan

S5-2 Isolation and culture of the hepatic cells from residual resected liver tissue

Kumiko Tatsumi1)2), Jumpei Kondo2), Masahiro Inoue2) and Yoshiharu Sakai1)

1) Department of Gastrointestinal Surgery, Graduate School of Medicine, Kyoto University, Japan


S5-3 Application of normal cells based on islet transplant research

Hirofumi Noguchi

Graduate School of Medicine, University of the Ryukyu, Japan

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S5-4 Dissecting the diversity of carcinogenesis pathways of pancreatic cancer using human tumor cell

resources including CTOS

Yusuke Mizukami

Department of Medicine, Asahikawa Medical University, Japan

S5-5 Application of cancer tissue-originated spheroid (CTOS) method

Masahiro Inoue

Osaka International Cancer Institute, Japan

S5-6 Construction of the medical BioBank for carring out large scale analysis utilizing AI

Akihiro Sekine1), Taiki Yamashita1), Ryo Takemura1), Yoichi Mashimo1) and Masahiro Inoue2)

1) Center for Preventive Medical Science, Chiba University, Japan


S5-7 Procurement of bio-resource for innovation of primary culture

Masahiro Inoue

Osaka International Cancer Institute, Japan

Supported by Safety Evaluation Forum 10:30～12:30 Symposium 6 Room C ( Conference Room : 3F)

Co-chair：Toshio Tanaka (Systems Pharmacology Mie University Graduate School of Medicine) Michio Fujiwara (Drug Safety Research Labs, Astellas Pharma Inc)

New horizons in alternatives with zebrafish to animal experiments S6-1 Global Development in emerging zebrafish-based “alternative” methods

Toshio Tanaka1)2)

1) Systems Pharmacology Mie University Graduate School of Medicine Tsu Mie Japan, Japan

2) Mie University Medical Zebrafish Research Center, Japan

S6-2 Efforts to optimize alternative methods for reproductive and developmental toxicity testing of drugs

in Japan and improve the predictability of zebrafish embryo toxicity test through harmonization of

international regulation

Michio Fujiwara

Drug Safety Research Labs, Astellas Pharma Inc., Tsukuba, Japan

S6-3 Application of genetically modified zebrafish for toxicity evaluation.

Akihito Yamashita

Sumitomo Dainippon Pharma Co., Ltd., Preclinical Research Laboratories, Japan

S6-4 Development of a genome-design medaka for an industrial application

Tomonori Deguchi

National Institute of Advanced Industrial Science and Technology (AIST)

Biomedical Research Institute, Advanced Genome Design Research Group, Japan

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S6-5 Establishment of brain ischemia-reperfusion model using zebrafish and evaluation of drug efficacy

Toshiaki Kume

Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan

Supported by Zebrafish Drug Discovery Society 12:45～13:30 Luncheon Seminar 2 Room A ( Convention Hall : 4F)

Chair：Masaharu Akita (Kamakura Women's University) LS2-1 Recent developments of toxicological alternative methods in China Shuangqing Peng1), Xingfen Yang2)3), JiaBin Guo1), Ying Yang3), Jin Li4), Qing Li3)

1) The Institute of Disease Control and Prevention, Academy of Military Medical, Sciences, Beijing, P.R. China, 2) School of Public Health, Southern Medical University, Guangzhou, P.R. China, 3) Guangdong Province Centre for Disease Control and Prevention, Guangzhou, P.R. China, 4) Unilevel, UK

LS2-2 Current activities for the development and validation of alternative test methods in Korea Tae Cheon Jeong1), Mi-Jeong Kang1), Mahesh R. Nepal1), Kyungyuk Ko2), Tae Sung Kim2), Jong Kwon

Lee2)

1) College of Pharmacy, Yeungnam University, Gyeongsan, Republic of Korea, 2) National Institute of Food and Drug Safety Evaluation, KFDA, Republic of Korea

Supported by the Cosmetology Research Foundation 12:45～13:30 Luncheon Seminar 3 Room B ( Convention Hall : 4F)

Chair: Takashi Sozu (Tokyo University of Science)

LS3-1 An in vitro co-culture system to assess lipophilic chemicals and petroleum chemicals for skin sensitization

Shujun Cheng1), Yu Chen2), Hua Shen3), Yihui Ke2) 1) Guangdong Inspection & Quarantine Technology Center, Guangzhou, China, 2) Guangzhou Chn-alt Biotechnology Co. Ltd., Guangzhou, China, 3) Shell International Petroleum Co. Ltd., Houston, USA LS3-2 An integrated testing strategy for in vitro skin irritation and corrosive assessment using EpiSkinTM skin

model Junming Huang1), Ying Yang1), Xingfen Yang1), Zhenzi Cai2), E Zhao2), Nan Li2), Qing Li1), Yanfeng Liu1), Meifen Chen1), Xikun Xiong1), Yangsheng Liang1), Caichun Ke1), Xin Chen1) 1) Guangdong Province Center for Disease Control and Prevention, Guangzhou, China, 2) L’Oreal China Research and Innovation Center, Shanghai

Supported by the Cosmetology Research Foundation

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14:00～16:00 Workshop1 Room A ( Convention Hall : 4F) Co-chair ：Naoki Yamamoto (Fujita Health University)

Sanae Takeuchi (P&G Japan) Irritation risk assessment applying data from nonanimal test methods W1-1 In vitro repeated exposure study using a three-dimensional cell culture model of human bronchial

tissue for assessment of aerosol chemicals

Shinkichi Ishikawa, Kazushi Matsumura, Nobumasa Kitamura, Yuichiro Takanami and Shigeaki Ito

Scientific Product Assessment Center, Japan Tobacco Inc., Japan

W1-2 Construction of 3D skin model using layer-by-layer cell manipulation and the future prospects as

alternatives to animal testing

Mitsuru Akashi

Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan

W1-3 Approach for detecting weak skin irritation due to cosmetic ingredients without using animal testing

Mariko Sugiyama

Shiseido Global Innovation Center, Japan W1-4 Applying the Depth of Injury Concept to Bovine Corneal Histopathology to Support Corneal

Recovery Predictions Hans Raabe Institute for In Vitro Sciences, Inc., USA

W1-5 Approach for assessing the mild irritants in the Bovine Corneal Opacity and Permeability Test

(BCOP Test)

Masatoshi Furukawa

Safety Research Institute for Chemical Compounds Co., Ltd., Japan

W1-6 Evaluation of corneal damage recovery using three-dimensional corneal re-construction model

Naoki Yamamoto

Division of Molecular Biology, Joint Research Support Promotion Facility, Center for Research

Promotion and Support, Fujita Health University, Japan 14:00～16:00 Symposium 7 Room B ( Convention Hall : 4F)

Co-chair：Masahito Ikawa (Research Institute for Microbial Diseases, Osaka University)Kazuto Watanabe (Japan Pharmaceutical Manufacturers Association)

Current status and problems of disease model animals leading to drug discovery S7-1 Progress and challenges of genome editing technology

Masahito Ikawa

Research Institute for Microbial Diseases, Osaka University, Japan

S7-2 ES/iPS cell-derived mammalian living models by using micro-fluidic devices

Yoh ichi Tagawa

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Tokyo Institute of Technology School of Life Science and Technology, Japan

S7-3 Utilization of human iPSC-derived cardiomyocytes and human iPSC-derived neurons for drug

discovery – Trial for microelectrode array data analysis using deep learning

Norimasa Miyamoto

Eisai Co., Ltd., Tsukuba Research Laboratories, Japan

S7-4 A humanized mouse model that is useful for basic medical research and new drug development

Hiroshi Suemizu

Central Institute for Experimental Animals, Japan

S7-5 Use of animal models for human disease in nonclinical safety assessment of new drugs

Masayuki Tomohiro1)2)

1) Non-clinical Evaluation Expert Committee, Drug Evaluation Committee, Japan Pharmaceutical

Manufacturers Association

2) Novartis Pharma K.K.

Supported by the Japanese Association for Laboratory Animal Science 14:00～16:00 Symposium 8 Room C ( Conference Room : 3F)

Co-chair：Yasunari Kanda (National Institute of Health Sciences) Kazuaki Nakamura (National Center for Child Health and Development)

Current status and future perspectives of good cell culture practice S8-1 Current status of GCCP in testing method

Yasunari Kanda

Division of Pharmacology, National Institute of Health Sciences, Japan

S8-2 Quality variability and good cell culture practice for human stem cells

Kusuda Miho Furue1)2)

1) Laboratory of Stem Cell Cultures, National Institutes of Biomedical Innovation, Health and

Nutrition, Japan

2) Stem Cell Business Development Section, Healthcare Business Unit, NIKON Corporation, Japan

S8-3 Good Cell Culture Practice in terms of Regenerative Medicine

Sachiko Ezoe

Osaka University Graduate School of Medicine, Japan

S8-4 Body on a Chip: Micro/Nanoengineered in vitro human model and its applications for Good Cell

Culture Practice

Kenichiro Kamei

Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Japan

Supported by the Japanese Tissue Culture Association 14:00～16:00 Workshop 2 Room D (Sub Exhibition Hall: 2F)

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Co-chair: Kunihiko Yamashita (Daicel Corporation) Yumiko Iwase (Life Science Institute, Inc., Mitsubishi Tanabe Pharma Corporation）

Forefront of the alternative research to animal testing in the cosmetic, chemical, food, and pharmaceutical companies W2-1 Importance and role of an alternative method for safety assessment in cosmetic development

Hidefumi Ikeda

Product Assurance Division, Mandom corp., Japan

W2-2 Importance and role of alternative to animal experiments in safety evaluation of chemical development

Toshihiko Kasahara

Fujifilm Corporation, Safety Evaluation Center, Ecology & Quality Management Csr. Div., Japan

W2-3 Expectation of the alternative methods for safety and security of food

Asuka Takumi

Institute for Innovation, Ajinomoto Co., Inc., Japan

W2-4 Current usage and future perspective of the alternative test methods in NISSIN FOODS Group.

Taisei Mizota

NISSIN FOODS HOLDINGS CO., LTD. Global Food Safety Institute, Japan

W2-5 The expected role of alternative methods in non-clinical drug safety assessment

Jumpei Kiyokawa and Tomoaki Inoue

Safety Assessment Department, Research Division, Chugai Pharmaceutical Co., Ltd., Japan

W2-6 The current state and issues of utilizing alternatives to animal experiments for safety evaluation of

drugs in our company

Motoki Umeda

Drug Development Research Laboratories, Maruho Co., Ltd., Japan

Supported by JSAAE Planning committee 16:10～16:30 Closing Ceremony Room D (Sub Exhibition Hall: 2F)

Poster presentation

P-1 Comparison of magnetic nanoparticles genotoxicities in in vivo and in vitro models: evaluating the

utility of substrata made from tissue/organ sections for histopathology-based systems

Shungo Saito1) 2), Tadashi Nittami1), Hiroshi Itagaki1), Yukari Totsuka2), Yohei Miyagi3)

and Masatoshi Watanabe1)4)

1) Graduate School of Engineering, Yokohama National University, Kanagawa, Japan

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2) Division of Carcinogenesis and Prevention, National Cancer Center Japan, Tokyo, Japan

3) Molecular Pathology & Genetics Division, Kanagawa Cancer Center Research Institute,

Kanagawa, Japan

4) Faculty of medicine, Mie University, Mie, Japan

P-2 Usefulness of three-dimensional cultured skin (LSE-high) in in vitro skin permeation study of

quasi-drugs

Takashi Maeda1), Kazutaka Nojimoto1), Katsuhiro Nakayama1), Takanori Kuramochi1), Hiroyuki

Takeuchi1), Motoki Hiramori1), Arata Watanabe2), Hiroaki Todo2) and Kenji Sugibayashi2)

1) R&D Department, Yokohama Laboratory Roman Industries Co., Ltd., Kanagawa, Japan

2) Faculty of Pharmaceutical Sciences, Josai University, Saitama, Japan

P-3 The methods to control spheroids formation speed on novel polymer and application for 3D cell culture

scaffold material. Naoto Ogiwara, Keisuke Kurauchi and Yuta Furumi TOYO INK SC HOLDINGS CO., LTD. Saitama, Japan

P-4 Pain responses of cultured human iPSC-derived sensory neurons using MEA system Aoi Odawara1)2)3), Naoki Matsuda2) and Ikuro Suzuki2) 1) Tohoku University 2) Tohoku Institute of Technology 3) JSPS

P-5 Study for environmental stress response of 3D reconstructed corneal models SkinEthicTM HCE

(Human Corneal Epithelium) Fumi Tsukui1), Yukiko Izutsu1), Misaki Hirayama2), Yuki Yamashita1) and Hitoshi Masaki2)

1) NIKKOL GROUP Nikoderm Research Inc., Osaka,Japan 2) Tokyo University of Technology, School of Bionics, Tokyo Japan

P-6 The protocol on "a test method for hepatic metabolism and excretion of chemicals" useful for the

technical transfer

Ayumi Oshikata and Toshiaki Takezawa

Division of Biotechnology, Institute of Agrobiological Sciences, National Agriculture and Food

Research organization

P-7 Development of novel in vivo estrogen-like effects screening assay using estrogen reporter "E-Rep"

mice.

Motoshi Furukawa, Kazuaki Horibe, Shinya Shimizu, Tsuyoshi Nakanishi and Hisamitsu Nagase Gifu

Pharmaceutical University, Gifu, Japan

P-8 Utility of 4CYPs-introduced HepG2 cells in mitochondrial toxicity evaluation in consideration of

metabolites

Shibin Kyu1), Moemi Kawaguchi1), Shuichi Sekine1), Akinori Takemura1), Daisuke Satoh2), Yasuhiro

Kazuki2), Mistuo Oshimura2), Toru Horie3) and Kousei Ito1)

1) Laboratory of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Chiba University

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2) Chromosome Engineering Research Center Tottori University

3) Drug Discovery and Development Institute

P-9 Development of a Novel Placental Drug Transport Assay Using Placental Cells Derived from Human

Embryonic Stem Cells and Induced Pluripotent Stem Cells.

Kouji Asano1), Noriyuki Suzuki1), Yasuhiko Takahashi1), Yoshitaka Tomigahara1) and Koichi Saito2)

1) Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd., Osaka, Japan

2) Advanced Materials Development Laboratory, Sumitomo Chemical Co., Ltd., Osaka, Japan

P-10 Establishment of stable effector cell line and application to antibody-dependent cellular cytotoxicity

assay for anti-TNFα antibody

Haruka Hirosaki1), Yosuke Maeda1)2), Hidenori Yamanaka1), Masahiro Takeyoshi1) and Hiroyuki

Iwata2)

1) Chemicals Assessment and Research Center, Chemicals Evaluation and Research Institute, Japan

2) The United Graduate School of Veterinary Science, Yamaguchi University

P-11 Multi-Immuno Tox Assay (MITA): the creation of its data set and the results of validation studies

Yutaka Kimura1), Rie Yasuno2), Mika Watanabe3), Miwako Kobayashi3), Tomoko Iwaki4),

Chizu Fujimura1), Yoshihiro Ohmiya2), Kohji Yamakage3), Yoshihiro Nakajima4), Mayumi

Kobayashi5), Takashi Omori5), Hajime Kojima6) and Setsuya Aiba1)

1) Department of Dermatology, Tohoku University Graduate School of Medicine, Sendai, Japan

2) Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology

(AIST), Tsukuba, Japan

3) Hatano Research Institute, Food and Drug Safety Center, Hadano, Japan

4) Health Research Institute, National Institute of Advanced Industrial Science and Technology

(AIST), Takamatsu, Japan

5) Division of Biostatistics, Department of Social/Community Medicine and Health Science, Kobe

University School of Medicine, Kobe, Japan

6) Japanese Center for the Validation of Alternative Methods, National Institute of Health Sciences,

Tokyo, Japan

P-12 Transient ischemia with activating mitochondrial function induces cell death by opening

mitochondrial transition pore

Yugo Ikeyama, Tomoyuki Sato, Shuichi Sekine, Koichi Arakawa, Kousei Ito

Laboratory of Biopharmaceutics, Faculty of Pharmaceutical Sciences, Chiba Univercity

P-13 Verification of the correlation between animal testing and in vitro evaluation system using 3D culture

scaffold Cellbed on drug resistance overcoming phenomenon

Taku Matsushita1), Kousuke Inamura1), Kazumi Emoto1), Takahiro Mizutami1), Yuji Komizu1),

Hideaki Ichihara1), Takuya Iwasa2), Kouhei Sasaki2), Rie Watanabe2), Masaaki Kawabe2) and Yoko

Matsumoto1)

1) Dept. of Applied Life Science, Sojo University, Kumamoto, Japan

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2) Central Research Laboratory, Japan Vilene Company, Ltd

P-14 Development of next generation 3D skin model; "Advanced SkinTM", for the evaluation of both

functional materials and cosmetic products.

Katsunari Tezuka1)2), Miho Ogawa1)2), Ayako Tsuchiya2), Shun Kimura2), Koh ei

Toyoshima1)2), Naokazu Okamoto1)2) and Takashi Tsuji1)2)

1) Organ Technologies Inc., Tokyo, Japan

2) RIKEN Center for Developmental Biology

P-15 Multi-laboratory validation study of ADRA as novel in chemico alternative test method for skin

sensitization: 2nd report

Ono Atsushi1), Shinichi Watanabe2), Tsunetsugu Sugawara2), Koji Wakabayashi3), Yu Tahara 3), Nobuyuki Horie4), Keiichi Fujimoto4), Kusakari Kei5), Yoshihiko Kurokawa5), Takashi

Sozu6), Takuto Nakayama6), Takeru Kusao6), Tsuyoshi Kawakami7), Kohichi Kojima8),

Hajime Kojima9), Jon Richmond10), Nicole Kleinstreuer11), Kim BaeHwa12), Yusuke Yamamoto１

3), Masaharu Fujita13), Kasahara Toshihiko13)

1) Okayama University, Graduate school of Medicine, Dentistry and Pharmaceutical sciences,

Okayama, Japan

2) LION CORPORATION, HUMAN & ENVIRONMENTAL SAFETY EVALUATION CENTER

3) MITSUI CHEMICALS, INC., Chemicals Safety Department

4) SUMITOMO Chemical Co., Ltd., Environmental Health Science Laboratory

5) NISSAN CHEMICAL INDUSTRIES, LTD. Biological Research Laboratories

6) Tokyo University of Science, Department of Information and Computer Technology, Faculty of

Engineering

7) National Institute of Health Sciences, Division of Environmental Chemistry

8) Food and Drug Safety Center, Hatano Research Institute

9) National Institute of Health Sciences, Biological safety Research Center, Division of Risk

Assessment

10) Dr. JonRichmond: Advice and Consultancy

11) NTP Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM)

12) College of Natural Sciences Keimyung University

13) FUJIFILM Corporation, Safety EvaluationCenter）

P-16 Novel skin sensitization test systems using peptide-immobilized polystyrene-microbeads Kenji Usui1), Hiroshi Miyazaki2), Yuuki Minamino1), Hideaki Mekata3), Masayuki Takaishi 3), Hidefumi Ikeda3), Kunihiko Yamashita2) and Yoshio Hamada1)

1) Konan University, Kobe, Japan 2) Daicel Corp., Himeji, Japan 3) Mandom Corp., Osaka, Japan

P-17 Evaluation of indirect activation of CAR in 3D-cultured HepG2 cells

Kaoru Kobayashi, Ikuko Azuma, Kosuke Yokobori, Kan Chiba and Hidetaka Akita

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Chiba University, Graduate School of Pharmaceutical Sciences, Chiba

P-18 Inkjet bioprinting applied to the development of in vitro cell-based assays

Waka Lin1), Natsuko Hemmi1), Shusaku Shiomoto1), Tomoaki Nakayama1), Yosuke Tabei2),

Masanori Horie2), Takanori Tano1) and Daisuke Takagi1)

1) RICOH COMPANY, LTD., Biomedical Research Department, Kanagawa, Japan

2) National Institute of Advanced Industrial Science and Technology, Health Research Institute

P-19 Establishment of a HepG2-NIAS cell line and its characteristics

Toshiaki Takezawa and Ayumi Oshikata

Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Tsukuba,

Japan

P-20 Basic study on the development of in vitro developmental toxicity tests using human iPS cell-derived

cranial neural crest-like cells

Mika Suga1)2), Sumiyo Mimura3), Yujung Liu2), Takako Nakano2), Hiroki Nikawa3) and Miho

K Furue2)

1) Department of Life Science Frontiers, Center for iPS Cell Research and Application (CiRA), Kyoto

University, Kyoto, Japan

2) Laboratory of Stem Cell Cultures, National Institutes of Biomedical Innovation, Health and Nutriton,

Osaka, Japan

3) Department of Oral Biology and Engineering, Graduate School of Biomedical and Health Sciences,

Hiroshima University, Hiroshima, Japan

P-21 Traumatic brain injury and aging in Drosophila melanogaster

Rikako Sanuki1)2)

1) Kyoto Institute of Technology, Institute for the Promotion of University Strategy Global Excellence

2) Kyoto Institute of Technology, Drosophila Genomics and Genetic Resource

P-22 Lead inhibits microglial cell migration via suppression of store-operated calcium entry Fei Zou, Jiawen Peng, Wei Tang, Yuhao Wang, Fan Zhou, Xiaojing Meng Department of Occupational Health and Occupational Medicine, School of Public Health, Southern Medical University, Guangzhou, Guangdong 510515, China

P-23 Enhanced Hepatocyte function by coculture with Fibroblast using 3D Silica Nonwoven Fabrics Shohei Ishikawa1), Yuki Takahashi2), Kohei Sasaki3), Masaaki Kawabe3) and Hidenori Otsuka 1)2)

1) Graduate School of Science, Tokyo University of Science, Tokyo, Japan 2) Department of Applied Chemistry, Faculty of Science, Tokyo University of Science, Tokyo, Japan 3) Central Research Laboratory, Japan Vilene Company Ltd., Koga, Japan

P-24 Characteristics of co-cultured hepatocytes in monolayer and spheroid cultures

Yusuke Ota, Hideo Yokoyama, Daisuke Miyamoto and Kohji Nakazawa

Department of Life and Environment Engineering, The University of Kitakyushu, Fukuoka, Japan

P-25 Monocyte culture using microwell chip

Tatsuya Kitajima, Daisuke Miyamoto and Kohji Nakazawa

16

Department of Life and Environment Engineering, The University of Kitakyushu, Fukuoka, Japan

P-26 Drug responsiveness of HepG2 spheroids using microwell chip culture

Daisuke Go, Yui Ishizaki, Daisuke Miyamoto and Khoji Nakazawa

The University of Kitakyushu, Fukuoka, Japan

P-27 Development of drug screening device using seesaw type bioreactor

Keito Iseki and Junji Fukuda

Yokohama National University, Kanagawa, Japan

P-28 Aerobic culture of multicellular spheroids by static oxygen supply method

Hirotaka Mihara1), Yasuyuki Sakai2) and Nobuhiko Kojima1)

1) Graduate School of Engineering, Yokohama City University

2) Graduate School of Engineering, University of Tokyo

P-29 Catch-and-release of target cells using electrochemical cell detachment

Junko Enomoto and Junji Fukuda

Faculty of Engineering, Yokohama National University, Japan

P-30 Spheroids of various cell types on oxygen-permeable plate

Kisaki Onishi and Junji Fukuda


P-31 Fabrication of In vitro blood vessel model with smooth muscle cell layer

Yuka Shimazu, Dina Myasnikova and Junji Fukuda

Faculty of Engineering, Yokohama National University, Yokohama, Japan

P-32 Fabrication of tailor-made cell sheets using micro stereolithography and electrochemical cell Transfer Yuka Kobayashi1), E.J. Christopher Cordonier2), Yohei Noda1), Hideo Honma2), Shoji Maru1) and Junji

Fukuda1)

1) Yokohama National University, Kanagawa, Japan

2) Kanto Gakuin University, Kanagawa, Japan

P-33 Evaluation of the effect of oxygen on human hepatocytes culture by using oxygen permeable

membrane

Keiichi Kimura1), Marie Shinohara2), Wenjin Xiao2) and Yasuyuki Sakai1)2)

1) Graduate School of Engineering, University of Tokyo

2) Institute of Industrial Science, University of Tokyo

P-34 Studies of cytotoxicity assays by using plant cultured cells

Riko Ito1), Hiroshi Itagaki2), Kazuyuki Hiratsuka1) and Shinichi Ogata1)

1) Graduate School of Environment and Information Sciences, Yokohama National University

2) Graduate School of Engineering, Yokohama National University P-35 Analysis of the combined effect of magnetic nanoparticles and docetaxel on prostate cancer cells

Kanako Kojima1), Tadashi Nittami1), Hiroshi Itagaki1) and Masatoshi Watanabe2)

17

1) Graduate School of Engineering, Yokohama National University, Kanagawa, Japan

2) Graduate School of Medicine, Mie University, Mie, Japan

P-36 Preparation method of cardiomyocytes tissue models by Microscopic Coating Applicator of needle

Method

Haruka Nakamura1)2)

1) NTN corporation New product development R&D center

2) Graduate School of Frontier Biosciences, Osaka University

P-37 Analysis of AtRad51 in response to DNA damage

Shinya Nanaumi, Norihito Kowada, Kazuyuki Hiratsuka and Shinichi Ogata

Graduate School of Environment and Information Sciences, Yokohama National University

P-38 Toward the Development of an in Vitro Allergy Test Device Integrated with an Electrochemical

Histamine Biosensor

Kikuo Komori, Masaharu Nakane and Yasuyuki Sakai

University of Tokyo

P-39 Hybrid liposomes inhibit the growth of liver cancer stem cells

Yuji Komizu1), Kousuke Inamura1), Seiichi Ishida2), Matsumoto Yoko1) and Matsushita Taku1)

1) Sojo University, Dept.of Applied Life Science, Kumamoto, Japan

2) National Institute of Health Sciences, Division of Pharmacology, Tokyo, Japan

P-40 Alternative to Animal Experiment for polypharmacy using chick embryos

Yuji Yoshiyama, Hideto Ariumi and Hiroyuki Miyazaki

Kitasato University School of Pharmacy

P-41 Multi-throughput multiorgan-on-a-chip platform using a pneumatic pressure-driven medium

circulation system

Shinji ugiura1), Taku Satoh1), Kazumi Shin1), Reiko Nagasaki1), Seiichi Ishida2), Kiyomi

Kikuchi3), Motoharu Kakiki3) and Toshiyuki Kanamori1)

1) National Institute of Advanced Industrial Science and Technology (AIST)

2) National Institute of Health Sciences

3) Eisai Co. Ltd.

P-42 Mass-production System of Spheroids by Combining Cluster Culture Device "TASCL" and Label-free

Image Analysis

Masashi Ikeuchi1)3), Chihiro Aoyama1) and Shuji Hayashi2)3)

1) Graduate School of Information Science and Technology, The University of Tokyo, Japan

2) The Foundation for Global Health Care, Japan

3) CYMSS Inc.

P-43 Analysis of Osteogenic Differentiation Behaviors of Bone Marrow-Derived Mesenchymal Stem Cells

Cultured in Silica Nonwoven Fabrics

Kazutoshi Iijima1), Shohei Ishikawa2), Mineo Hashizume1) and Hidenori Otsuka2)

18

1) Faculty of Engineering, Tokyo University of Science, Tokyo, Japan

2) Graduate School of Science, Tokyo University of Science, Tokyo, Japan

P-44 1,2-Dichloroethane induces reproductive toxicity mediated by the CREM/CREB signaling pathway in

male NIH Swiss mice

Zhenlie Huang1), Yating Zhang2), Xingfen Yang3)

1)Department of Toxicology, Guangdong Provincial Hospital for Occupational Disease Prevention and

Treatment, Guangzhou, China

2)School of Public Health, Guangdong Pharmaceutical University, Guangzhou, China

3)School of Public Health, Southern Medical University, Guangzhou, China

P-45 Development of in silico model for predicting idiosyncratic drug-induced hepatotoxicity risk

Kosuke Imai and Atsushi Ono

Faculty of Pharmaceutical, Okayama University

P-46 Evaluation of the performance measures used in the validation for the h-CLAT and DPRA methods

Takuto Nakayama1) and Takashi Sozu2)

1) Department of Management Science, Tokyo University of Science Graduate School of Engineering

2) Department of Information and Computer Technology, Faculty of Engineering, Tokyo University

of Science

P-47 Development of in vitro hepatotoxicity assessment system to predict the toxicological potential of

cosmetic raw materials

Takumi Nukaga1)2), Moemi Kawaguchi2), Shuichi Sekine2), Akinori Takemura2), Takeshi

Susukida2), Morihiko Hirota1), Shiho Oeda1), Tomohiko Miyagawa1), Akiko Tamura1),

Atsushi Kodama1), Kousei Ito2) and Hirokazu Kouzuki1)

1) Global Innovation Center, Shiseido

2) Graduate school of Pharmaceutical Sciences, Chiba University, Japan

P-48 Comparison of basal and induced activities of various types of Cytochrome P450 enzymes in PXB-

able™ and 3D cultured primary human hepatocyte on Cell-able plate

Tomoko Jomura1), Yuki Akahira1), Chihiro Yamasaki2), Yuji Ishida2)3) and Chise Tateno2)3)

1) TOYO GOSEI CO., LTD. Photosensitive Materials Research Center Biotech Applications Group

2) PhoenixBio Co., Ltd.

3) Liver Research Project Center in Hiroshima

P-49 Evaluation of uptake/efflux transporter expression and function in ECM loaded spheroid

Fumiya Tao, Yuki Asakura and Nobuhiko Kojima

Yokohama City University, Yokohama, japan

P-50 Comparative metabolome analysis and toxicity test of cultured fetal and adult hepatocytes in humans

(4th Report)

Asuka Shiraki1), Kousuke Inamura1), Takahiro Mizutami1), Yuji Komizu1), Su Ryang Kim2),

Seiichi Ishida2) and Taku Matsushita1)

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1) Department of Applied Life Science, Sojo University, Kumamoto, Japan

2) Division of Pharmacology, National Institute of Health Sciences, Tokyo, Japan

P-51 In vitro reproduction of drug resistance phenomena and biliary excretion of HepG2 cells using 3D

culture scaffold, Cellbed

Maya Nakamura1), Takahiro Mizutami1), Yuji Komizu1), Takuya Iwasa2), Kouhei Sasaki2), Rie

Watanabe2), Masaaki Kawabe2), Seiichi Ishida3) and Taku Matsushita1)

1) Dept. of Applied Life Science, Sojo University, Kumamoto, Japan

2) Central Research Laboratory, Japan Vilene Company, Ltd., Ibaraki, Japan

3) Division of Pharmacology, National Institute of Health Sciences., Tokyo, Japan

P-52 Evaluation in functional expression and maintenance culture of cryopreserved human hepatocyte using

Cellbed as 3D scaffold

Hanaka Nagasaki1), Takahiro Mizutami1), Yuji Komizu1), Takuya Iwasa 2), Kouhei Sasaki 2),

Rie Watanabe2), Masaaki Kawabe2), Seiichi Ishida3) and Taku Matsushita1)

1) Dept. of Applied Life Science, Sojo University, Kumamoto Japan

2) Central Research Laboratory, Japan Vilene Company, Ltd, Ibaragi, Japan.

3) Division of Pharmacology, National Institute of Health Sciences, Tokyo, Japan

P-53 The three-dimensional reconstructed corneal model using an immortalized human corneal epithelial cell

line(iHCE-NY) on alternative eye irritation test method.

Noriko Hiramatsu1), Naoki Yamamoto2), Yoshinao Kato3), Atsushi Sato3), Sumito Isogai4),

Kazuyoshi Imaizumi4), Atsuhiro Tanikawa5), Koji Hirano5), Masayuki Horiguchi5) and Hajime

Kojima6)

1) The Graduate School of Medicine, Fujita Health University, Aichi, Japan

2) Division of Molecularbiology, Joint Research Support Promotion Facility, Fujita Health University,

Aichi, Japan

3) Nippon Menard Cosmetic Co., Ltd., Aichi, Japan

4) Department of Respiratory Medicine, School of Medicine, Fujita Health University, Aichi, Japan

5) Department of Ophthalmology, School of Medicine, Fujita Health University, Aichi, Japan

6) National Institute of Health Sciences (NIHS), Tokyo, Japan P-54 Addressing the exceptions of the applicability domain for highly volatile substances in the Short Time

Exposure (STE) test method for assessing eye irritation potential

Takayuki Abo1)2), Takuo Yuki1), Daisuke Araki1), Yutaka Takahashi1), Hitoshi Sakaguchi1) and Hiroshi

Itagaki2)

1) Kao Corporation, R&D - Safety Science Research, Kanagawa, Japan

2) Yokohama National University, Graduate School of Engineering, Kanagawa, Japan

P-55 Photosafety assessment of dermally applied chemicals by combined use of photochemical and

pharmacokinetic data

Yoshiki Seto, Hiroto Ohtake, Hideyuki Sato and Satomi Onoue

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Department of Pharmacokinetics and Pharmacodynamics, School of Pharmaceutical Sciences,

University of Shizuoka, Shizuoka, Japan

P-56 Study of in vitro phototoxic test using a 3D human epidermal skin model (LabCyte)

Mami Kawai1), Satoko Aoki2), Hiroko Okutomi2), Mika Watanabe2), Kohji Yamakage2) and

Amane Inoue1)

1) Rohto Pharmaceutical Co., Ltd.

2) Hatano Research Institute, Food and Drug Safety Center

P-57 Construction of 3D skin model using layer-by-layer cell coating technology and their utilization as

skin irritation tests

Takami Akagi and Mitsuru Akashi

Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan

P-58 Study of in vitro irritation testing in feminine hygene products using a reconstructed human vaginal

mucosa epithelium model (part 2)

Shoko Koizumi, Hidefumi Ikeda and Masayuki Takaishi

Product Assurance Division, Mandom Corp., Osaka, Japan

P-59 Evaluation of Mild Irritant Based on the Pathological Findings on the Cornea in the Bovine Corneal

Opacity and Permeability Test (BCOP Test, an Alternative Method of Eye Irritation Test)

Masatoshi Furukawa1), Takashi Sakakibara1), Kouta Itoh1), Masao Matsuura1) and Hajime

Kojima2)

1) Safety Research Institute for Chemical Compounds Co., Ltd., Sapporo, Japan


P-60 Use of in silico methods in skin corrosion and irritation IATA

Atsutoshi Abe, Takuhito Sezaki and Katsutoshi Kinoshita

Mitsui Chemicals, Inc., Responsible Care & Quality Assurance Division, Chemicals Safety Department

P-61 Contributions of CYP1A1, 1A2 or 2B6 to the focus formation in Bhas42 cell transformation assay

with 3-methylcholanthrene

Takashi Hirooka1), Keiko Abe1)2) and Kiyomi Ohmori3)

1) KISTEC

2) Graduate School of Agricultural and life Science, Tokyo University

3) Kanagawa Prefectural Institute of Public Health

P-62 Non-genotoxic carcinogen-induced changes in gene expression on Bhas 42 cell transformation assay

Kiyomi Ohmori1), Asuka Kamei2), Yuki Watanabe2) and Keiko Abe 2)3)

1) Kanagawa Prefectural Institute of Public Health

2) Kanagawa Institute of Industrial Science and Technology

3) The University of Tokyo

P-63 Statistical formulation for between-laboratory reproducibility in validation of alternative test methods

Takashi Sozu1), Takuto Nakayama2) and Hitomi Sato3)

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1) Department of Information and Computer Technology, Faculty of Engineering, Tokyo University

of Science

2) Department of Management Science, Tokyo University of Science Graduate School of Engineering

3) Department of Management Science, Faculty of Engineering, Tokyo University of Science

P-64 Comparison of animal experimentation protocols between US and Japan: How Japanese protocols can

be improved

Akishige Fujisawa

Specified Nonprofit Corporation All Life In a Viable Environment

P-65 Me-too Validation Study of Reconstructed Human Epidermal Model, LabCyte EPI-MODEL24 Skin

Corrosivity Test Method

Hajime Kojima1), Azusa Mori2), Mayumi Kobayashi2), Nobuyuki Shinoda4), Saori Hagiwara4), Yusuke

Yamamoto5), Toshihiko Kasahara5), Noriko Yamaguchi6), Ryosuke Sato6), Takayuki Fukuda6),

Alessandro Wataru Amici7), Masakazu Kato7), Nana Mashita3) and Takashi Omori2)


2) Kobe University School of Medicine

3) Doshisha University

4) Drug Safety Testing Center Co., Ltd.,

5) Fujifilm Corporation,

6) BOZO Research Center Inc.

7) Japan Tissue Engineering Co., Ltd.

P-66 Establishment and application of immortalized human epidermal keratinocytes as an in vitro alternative

for skin irritation test

Cho-Won Kim1), Eui-Bae Jeung2), and Kyung-Chul Choi1)

1) Laboratory of Biochemistry and Immunology

2) Laboratory of Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk

National University, Cheongju, Chungbuk, Republic of Korea

P-67 Development of a novel in chemico alternative test method for skin sensitization (ADRA) without

using molecular weight for test chemical solution

Yusuke Yamamoto, Toshihiko Kasahara, Yasuhiro Katsuoka and Masaharu Fujita

Safety Evaluation Center, Ecology&Quality Management Division, Csr Division, Kanagawa, Japan

P-68 Study of factorial analysis and countermeasure of oxidation of Cys-derivative reagent (NAC) using in

the ADRA (Amino acid Derivative Reactivity Assay) as the alternative test method for skin sensitization.

Masaharu Fujita1), Yusuke Yamamoto1), Shinichi Watanabe2), Tsunetsugu Sugawara2), Koji

Wakabayashi3), Yu Tahara3), Nobuyuki Horie4), Keiichi Fujimoto4), Kei Kusakari5), Yoshihiko

Kurokawa5), Tuyoshi Kawakami6), Kohichi Kojima7), Hajime Kojima8), Atsushi Ono9) and

Toshihiko Kasahara1)

1) Safety Evaluation Center, Ecology and Quality Management Division, CSR Division, FUJIFILM

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Corporation.

2) LION Corporation, HUMAN and ENVIRONMENTAL SAFETY EVALUATION CENTER

3) MITSUI CHEMICALS, INC., Chemical Safety Department Responsible Care and Quality Assurance

Division

4) SUMITOMO CHEMICAL Co., Ltd., Environmental Health Science Laboratory

5) NISSAN CHEMICAL INDUSTRIES, LTD., Toxicology and Environmental Science Department

Biological Research Laboratories

6) National Institute of Health Sciences, Division of Environmental Chemistry

7) Food and Drug Safety Center, Hadano Research Institute

8) National Institute of Health Sciences, Biological Safety Research Center,

9) Okayama University, Graduate school of medicine, Dentistry and Pharmaceutical Sciences, Division

of Pharmaceutical Sciences

P-69 The effect of the changes in the prediction model of in vitro skin sensitization test; SH test

Nana Kuwata, Morihiko Hirota, Hayato Nishida and Hirokazu Kouzuki

SHISEIDO CO., LTD, Yokohama-city, Japan

P-70 The feasibility of technology transfer of in vitro sensitization test, SH test (Part 3)

Akemi Toyoda1), Kaoru Kasahara1), Maki Sugiyama1), Masaharu Kurosawa2), Midori

Takeyoshi2), Sakiko Aizawa2), Noriyasu Imai2) and Morihiko Hirota3)

1) POLA Chemical Industries, inc., Yokohama, Japan

2) KOSE Corporation, Tokyo, Japan

3) Shiseido Global Innovation Center, Yokohama, Japan

P-71 Predictive performance of in vitro skin sensitization assay using reconstructed human epidermis;

EpiSensA (Part1; Lipophilic chemicals)

Hideyuki Mizumachi1), Megumi Sakuma2), Noriyasu Imai2) and Masaaki Miyazawa1)

1) Kao Corporation, Safety Science Research Laboratory, Tochigi, Japan

2) KOSE Corporation, Fundamental Research Laboratories

P-72 Predictive performance of in vitro skin sensitization assay using reconstructed human epidermis;

EpiSensA (Part2; Pre/pro-haptens)

Megumi Sakuma1), Hideyuki Mizumachi2), Masaaki Miyazawa2) and Noriyasu Imai1)

1) KOSE Corporation, Fundamental Research Laboratories, Tokyo, Japan

2) Kao Corporation, Safety Science Research Laboratory

P-73 Development of in vitro skin sensitization test method based on Nrf2-keap1-ARE pathway with transient

expression system

Yosuke Maeda1)2), Haruka Hirosaki1), Hidenori Yamanaka1), Makoto Nakai1), Masahiro

Takeyoshi1) and Hiroyuki Iwata2)

1) Chemicals Assessment and Research Center, Chemicals Evaluation and Research Institute, Japan

2) The United Graduate School of Veterinary Science, Yamaguchi University

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P-74 Safety assessment for skin sensitization by plants extracts using modified h-CLAT Ⅰ. -high-

concentration short term exposure method―

Yuimi Matsuoka1), Tomohisa Yoshimura1), Tohru Nagahama1), Hiroyuki Kojima2), Makoto Tsuboi2)

and Hiroshi Itagaki3)

1) TAISHO PHARMACEUTICAL Co., Ltd. Tokyo, Japan

2) ICHIMARU PHARCOS Co., Ltd. Gifu, Japan

3) Yokohama National University, Kanagawa, Japan

P-75 Safety assessment for skin sensitization by plant extracts using modified h-CLAT II. Using extract

models containing chemicals from natural sources

Yuki Ishii, Kazuto Narita and Hiroshi Itagaki

Yokohama National University

P-76 Study of linalool-containing essential oil using skin sensitization test alternative method

Maki Doi1)2), Hideto Ariumi1) and Yuji Yoshiyama1)

1) Kitasato University School of Pharmacy, Tokyo, Japan

2) Pharmaholding Corporation

P-77 Influence of culture medium and cell-cell interactions between THP-1 cells and NHEK cells on the

expression of protein markers of immunotoxicity

Yuka Sawada, Hanae Tsukumo, Junji Fukuda and Hiroshi Itagaki


P-78 Development of an In Vitro Test for the Allergenic Potency of Proteins I. Investigation of false

positive reactions

Dan Xie1), Hanae Tsukumo1)2), Kunihiko Yamashita3), Hajime Kojima2) and Hiroshi Itagaki1)

1) Yokohama National University


3) DAICEL Corporation

P-79 Development of an In Vitro Test for the Allergenic Potency of Proteins II. Exclusion of the effect of

LPS in test proteins

Hanae Tsukumo1)2), Dan Xie1), Kunihiko Yamashita3), Hajime Kojima2) and Hiroshi Itagaki1)



3) DAICEL Corporation

P-80 Development of an In Vitro Test for the Allergenic Potency of Proteins III. Investigation of the

usefulness of differentiated THP-1 cells

Akane Oka, Hanae Tsukumo, Shinichi Ogata and Hiroshi Itagaki


P-81 In vitro detection for protein allergen inducing type 1 allergy via the skin route

Yasutaka Kuroda1)2), Takuo Yuki1), Yutaka Takahashi1), Hitoshi Sakaguchi1), Kayoko Matsunaga

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3) and Hiroshi Itagaki2)

1) Kao corporation Safety Science Research Laboratories, Tochigi, Japan

2) Yokohama National University Graduate School of Engineering

3) Fujita Health University School of Medicine Department of Integrative Medical Science for Allergic

Disease

P-82 Second report on reducing false negative results of an in vitro skin sensitization test—human cell line

activation test

Kazuto Narita1)2), Yuuki Ishii1), Hajime Kojima2) and Hiroshi Itagaki1)



P-83 The effects of mixtures of chemicals on in vitro skin sensitization test Ⅱ

Minori Mezaki, Takafumi Mitachi, Mai Kouzui, Ryo Maruyama and Hiroshi Itagaki


P-84 Effect of intracellular ROS on h-CLAT

Ryo Maruyama, Takafumi Mitachi, Minori Mezaki, Mai Kouzui and Hiroshi Itagaki


P-85 Examination of the influence of NLRP3 inflammasome in in vitro skin sensitization test Mai Kouzui1), Takafumi Mitachi1), Minori Mezaki1), Ryo Maruyama1), Hajime Kojima2)


1) Yokohama National University 2) National Institute of Health Sciences

P-86 Study on key factors that influence the CD86 and CD54 expression in THP-1 cells

Takafumi Mitachi1), Minori Mezaki1), Mai Kouzui1), Ryo Maruyama1), Hajime Kojima2)




P-87 Skin sensitization studies by quantitative structure-activity relationships (QSAR) approach

Kazuhiro Sato1), Jose Ciloy2) and Yukinori Kusaka1)

1) Department of Environmental Health, School of Medicine, University of Fukui

2) Fujitsu Kyushu System Services Limited

P-88 A novel in vitro assay for sensitisers in a purely aqueous system: the modified IL-8 Luc assay using X-

VIVOTM 15 as a solvent

Yutaka Kimura1), Mika Watanabe2), Noriyuki Suzuki3), Tomoko Iwaki4), Kohji Yamakage2),

Koichi Saito5), Yoshihiro Nakajima4), Chizu Fujimura1), Yoshihiro Ohmiya6), Takashi Omori 7), Hajime Kojima8) and Setsuya Aiba1)

1) Department of Dermatology, Tohoku University Graduate School of Medicine, Sendai, Japan

2) Hatano Research Institute, Food and Drug Safety Center, Hadano, Japan

3) Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd., Osaka, Japan

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4) Health Research Institute, National Institute of Advanced Industrial Science and Technology

(AIST), Takamatsu, Japan

5) Advanced Materials Development Laboratory, Sumitomo Chemical Co., Ltd., Osaka, Japan

6) Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology

(AIST), Tsukuba, Japan

7) Division of Biostatistics, Department of Social/Community Medicine and Health Science, Kobe

University School of Medicine, Kobe, Japan

8) Japanese Center for the Validation of Alternative Methods, National Institute of Health Sciences,

Tokyo, Japan

R-1 Report of International committee in JSAAE

Hajime Kojima1), Kurosawa Tsutomu2)9), Suzuki Makoto3), Takeyoshi Masahiro4), Kanda Yasunari1), Takeuchi Sanae5), Sakuma Megumi6), Nakamura Maki7), Sozu Takashi8)


2) Kagoshima University

3) Okinawa International University

4) Chemicals Evaluation and Research Institute

5) P&G Japan

6) KOSE Corporation

7) Kobayashi Pharmaceutical Co., Ltd.

8) Tokyo Univ. Sci.

9) Animal Welfare Research Japan

Program - UMIN

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