PLIDENTA PRO-T-ACTION

Bite down on cavities!NEW GENERATION ANTI-CAVITY TOOTHPASTES

CARIES EPIDEMIOLOGY & MICROBIOLOGICAL SITUATION

Caries is the most common infectious disease

More than 5 bn. people suffer from tooth decay

Epidemiological studies1 show an alarming globalincrease in caries prevalence

Caries is very strongly associated with colonization of the tooth surface biofilm by mutan-producing streptococci.

Most common: Streptococcus mutans

1: The global increase in dental caries. A pending public health crisis; Am.J.Dent. 22, 2009, 3-8

Tooth surface

Mutan

S. mutans

Agglutinin

WHAT IS L. PRO-T-ACTION?

Lactobacillus Pro-t-action is a specifically acting probiotic (Lacobacillus paracasei) which co-aggregates mutans Streptococci and thus reduces caries-causing bacteria in the mouth.

Lactobacillus Pro-t-action is a trademark of BASF Future Business GmbH, Germany

It is scent-free and taste-free in the toothpaste, it does not irritate the mucous membrane and it contributes to the maintenance of healthy and useful microflora in the mouth with regular daily use.

Plidenta Pro-t-action is the first toothpaste in the world to contain this probiotic culture.

Scanning electron microscope,4000 x magnification

Scanning electron microscope,21000 x magnification

HOW IT WORKS IN TOOTHPASTE?

Acid eats away at tooth enemal and caause cavities

Pro-t-action collects bad bacteria (S. Mutans)

Caries is very strongly associated with colonization of the tooth surface biofilm by mutan-producing streptococci. Most common: Streptococcus mutans

Lactobacillus Pro-t-action selectively gathers and groups Streptococcus mutans bacteria

By teeth washing the bacteria are flushed out of the mouth

Sodium Fluoride strengthens tooth enamel

HOW IT LOOKS?

Microscopy: phase contrast and fluorescent labelling

AggregatesS. mutans LB pro-t-action®

PLIDENTA PRO-T-ACTION

Prevents plaqueContributes to the removal of the Streptococcus mutans bacteria, the most common cause of cavitiesCreates an environment for the development of good bacteriaReacts exclusively with cavity-causing bacteria, but does not affect other bacteria in the mouth Acts already after 15 seconds of brushingAccording to scientific research presented at international professional conferences, Lactobacillus Pro-t-action decreases the number of S. mutansbacteria, which is considered to be the most responsible for cavities, by up to 50% with regular daily use.Prevents the accumulation of plaqueStrengthens tooth enamel

CLINICAL RESEARCH

MARTINA MAJSTOROVIC, Associate Professor, DUBRAVKA NEGOVETIC VRANIC, Assistant ProfessorSchool of Dental Medicine, University of Zagreb, Department of Paediatric Dentistry

Efficacy of probiotic toothpaste (Plidenta Pro-t-action, Neva, Croatia) related to the presence of cariogenic bacteria in the oral cavity

There was a statistically significant decrease in the number of participants with a very high number of streptococcus (78.4% vs.26.5%) and lactobacillus (52.9% vs. 26.5%) after 4 weeks of using Plidenta toothpaste. As to gender, no statisticallly significant difference was found neither with respect to the number of Sreptococci and Lactobacilli, or the pH status. Conclusion: Regular usage of tested toothpaste reduces the number of cariogenic bacteria in the saliva.

REF. JOURNAL PUBLICATIONS

LANG C, BÖTTNER M, HOLZ C, VEEN M, RYSER M, REINDL A, POMPEJUS M and TANZER JM. Specific Lactobacillus/Mutans Streptococcus co-aggregation. (2010) J Dent Res. 89(2): 175-9

TANZER JM, THOMPSON A, LANG C, COOPER B, HARENG L, GAMER A, REINDL A, and POMPEJUS M.Caries inhibition by and safety of Lactobacillus paracasei DSMZ16671. (2010) J Dent Res. 89(9): 921-6.

REF. CONFERENCE PUBLICATIONSC. LANG, M. BOETTNER, J. TANZER, A. REINDL, and M. POMPEJUSLactobacillus paracasei DSM16671 Specifically Co-aggregates with Mutans Streptococci (2009) J Dent Res 88 (Spec Iss A): 143

J. TANZER, A. THOMPSON, C. LANG, M. BOETTNER, A. REINDL, and M. POMPEJUSPasteurized Probiotic Lactobacillus paracasei Inhibits Streptococcus mutans Colonization, and Caries (2009) J Dent Res 88 (Spec Iss A):144

M. POMPEJUS, B. COOPER, C. LANG, J. TANZER, A. REINDL, and L. HARENGStreptococcus mutans aggregating Lactobacillus paracasei DSM16671 is safe to use (2009) J Dent Res 88 (Spec Iss A):1189

M. CHAPAT, M. HANNIG, S. RUPF, M. HENKES and M. POMPEJUS Influence of heat-killed Lactobacillus paracasei on biofilm formation in situ(2011) J Dent Res 90 (Spec Iss A): 2659

M. POMPEJUS, C. ALEXANDER, C. HOLZ, C. BALCKE, L. WONG and C. LANGReduction of salivary mutans streptococci levels by Lactobacillus paracasei DSMZ16671 (2011) J Dent Res 90 (Spec Iss A): 2762

REF. CLINICAL RESEARCHMARTINA MAJSTOROVIC, Associate Professor, DUBRAVKA NEGOVETIC VRANIC, Assistant ProfessorSchool of Dental Medicine, University of Zagreb, Department of Paediatric Dentistry

Efficacy of probiotic toothpaste (Plidenta Pro-t-action, Neva, Croatia) related to the presence of cariogenic bacteria in the oral cavity

Objective: The aim of this study was to evaluate potential benefits from using a probiotic toothpaste (Plidenta Pro-t-action, Neva, Croatia) in decreasing the number of cariogenic bacteria (Streptococcus mutans and Lactobacillus) that are normally present in the mouth. The purpose was also to evaluate a potential influence of toothbrushing on the saliva buffer capacity. Materials and methods: This research study was conducted on a sample of 50 patients over a 4 week time frame interval. CRT tests were individually applied to each participant included in the study at the beginning as well as at the 2 and 4 week intervals during which the patients were instructed to brush their teeth by using Plidenta toothpaste. The number of Streptococcus mutans and Lactobacillus was taken from saliva and counted after 48 hours. Buffer capacity of saliva was measured for each patient at the 2 and 4 week check-ups. Patients were provided with the toothbrushes of medium hardness and were instructed to brush their teeth thouroughly 2 times a day by using Plidenta probiotic toothpaste. Patients were in the age group between 20 and 24 years of age equally divided by genders. Results:There was a statistically significant decrease in the number of participants with a very high number of streptococcus (78.4% vs.26.5%) and lactobacillus (52.9% vs. 26.5%) after 4 weeks of using Plidenta toothpaste. As to gender, no statisticallly significant difference was found neither with respect to the number of Sreptococci and Lactobacilli, or the pH status. Conclusion: Regular usage of tested toothpaste reduces the number of cariogenic bacteria in the saliva.

M. CHAPAT, M. HANNIG, S. RUPF, M. HENKES and M. POMPEJUS Influence of heat-killed Lactobacillus paracasei on biofilm formation in situ(2011) J Dent Res 90 (Spec Iss A): 2659

Objectives: Mutans streptococci are important cariogenic pathogens. Lactobacillus paracasei DSMZ16671 specifically co-aggregates with mutans streptococci. The aim of this in situ study was to investigate 1) the adherence of heat-killed L. paracasei DSMZ16671 on pellicle covered enamel surfaces, 2) their adherence on matured biofilms and, 3) the influence on the amount of Streptococcus mutans in saliva. Methods: Enamel slices of bovine incisors (5x5x2 mm) were exposed intraorally (6 participants) for 3 min up to 48 h for pellicle and for biofilm formation. Participants rinsed with L. paracasei mouthrinse (107/ml) or placebo mouthrinse for 30 s every 12 h. Paraffin stimulated saliva samples were taken after 24 h and after 48 h before rinsing. The vitality of the biofilms was compared by live/dead staining after rinsing with L. paracasei mouthrinse or placebo mouthrinse. Biofilm morphology was visualized by scanning-electron-microscopy (SEM) assessing the occurrence of rod-shaped bacteria. Saliva samples were plated on MSB agar with bacitracin and mutans streptococci amounts were determined. All experiments were carried out in triplicates. Results: Biofilm amounts and viability of biofilms were comparable for L. paracasei mouthrinse and placebo mouthrinse. However, in SEM the ratio of rod-shaped bacteria was slightly increased in biofilms exposed to L. paracasei mouthrinse. The amount of mutans streptococci in saliva samples was reduced by 50 percent after using L. paracasei mouthrinse. Conclusions: The results indicate reduction of salivary levels of mutans streptococci and no adverse effects in dental biofilms under the influence of mouthrinses with heat-killed L. paracasei. The application of this heat killed probiotic strain could enable new strategies for caries control by reduction of mutans streptococci in the oral cavity

M. POMPEJUS, C. ALEXANDER, C. HOLZ, C. BALCKE, L. WONG and C. LANGReduction of salivary mutans streptococci levels by Lactobacillus paracasei DSMZ16671 (2011) J Dent Res 90 (Spec Iss A): 2762

Objectives: One important strategy to prevent tooth decay can be the reduction of mutans streptococci levels in the oral cavity. Lactobacillus paracasei DSMZ16671 has been shown to specifically co-aggregate mutans streptococci in-vitro and to inhibit caries in rats. Purpose of this study is to evaluate the functionality of L. paracasei DSMZ16671 in the oral cavity of healthy humans using sugar-free candies as delivery vehicle. Methods: A double-blind, randomized, placebo-controlled clinical trial with 60 healthy adults was set-up. 2 different concentrations of L. paracasei DSMZ16671 or placebo were delivered in sugar-free hard candies. Saliva samples were collected immediately before and after consumption of a single sugar-free candy. Mutans streptococci levels were determined by cultivation on selective growth media. Results: The group consuming placebo candies showed no changes in the salivary level of mutans streptococci. In contrast, both test groups consuming candies containing L. paracasei DSMZ16671 showed reduction of the salivary level of mutans streptococci in a dose-dependent manner. Conclusions: The data provide evidence that L. paracasei DSMZ16671 delivered in sugar-free candies is effectively reducing salivary mutans streptococci levels. Use of L. paracasei DSMZ16671 in sugar-free candies is suggested as an attractive way of daily oral hygiene.

CREDENTIALS

Prof. Dr. Ch. Lang - Presentations on the 8th Asia-Pacific Weeks (APW) in Berlin

CREDENTIALS

Datamonitor Product Launch Analitics by Symrise Quarterly Report, New Launch Alert , July 2011

CREDENTIALS

Declaration of Ministry of Health and Welfare of the Republic of Croatian as COSMETIC WITH SPECIAL EFFECTS (PURPOSES)

“HELPS TO PREVENT TOOTH DECAY CAUSED BY BACTERIA S- MUTANS AND THE CREATION OF DENTAL PLAQUE AS WELL AS STRENGHTENES OF TOOTH ENAMEL”

CREDENTIALS

Declaration of Ministry of Health B&H as COSMETIC WITH SPECIAL EFFECTS (PURPOSES)

“HELPS TO PREVENT TOOTH DECAY CAUSED BY BACTERIA S- MUTANS AND THE CREATION OF DENTAL PLAQUE AS WELL AS STRENGHTENES OF TOOTH ENAMEL”

CREDENTIALS

TECHNOLOGY REVIEW

CREDENTIALS

Martina Majstorovic, Associate Professor, Dubravka Negovetic Vranic, Assistant ProfessorSchool of Dental Medicine, University of Zagreb, Department of Paediatric Dentistry, Authors Plidenta Pro-t-action Clinical Study

“The clinical research results conducted on this sample have suggested that regular daily use of Plidenta’s new toothpaste with probiotic bacteria, whose mechanism is based on selective removal of cariogenic bacteria, contributes to reducing the risk of caries. So this toothpaste represents the most innovative product currently available on our toothpaste market, whose regular use can help achieve a significant effect in the prevention of cavities. It acts as an excellent therapeutic medium which leads to the optimal ratio of good and bad bacteria in the saliva which represents a significant innovation in preventive dentistry, especially in patients at high risk for cavities and with a large number of potentially pathogenic bacteria in their mouths,”

Nutraingredients.com http://www.nutraingredients.com/content/view/print/317675

Tooth-friendly probiotic products are on horizon, BASF “… Dr Christine Lang from OrganoBalance told delegates at the International Probiotics Association's (IPA) congress in Miami this year that the concept of probiotics for oral health centred on S.mutans as the main bacteria associated with tooth decay. Lang explained that the bacteria bind to teeth forming dental plaque, which then converts sugar into acid that attacks tooth enamel. Lang and her team screened over 700 Lactobacillus strains, and found that Lactobacillus paracasei aggregated best with S.mutans, clumping bacteria together and allowing easy removal from the mouth via swallowing or rinsing.

CREDENTIALS

Prof. Dr. Jagoda Šuškovid, Food Technology and Biotechnology, University of Zagreb, Laboratory for antibiotics, enzymes, probiotics and starter cultures

“One way of combating cavities and application of probiotic strains that can interfere with the bacterium Streptococcus mutans adhesion prevention and the creation of dental plaque biofilms. Based on this mechanism of action has recently marketed toothpaste Plidenta Pro-t-action containing the probiotic bacterium effective to the pathogenic bacterium Streptococcus mutans. This product is a result of the world's scientific knowledge (company BASF - Future Business GmbH, Deutschland) and our local knowledge (of Neva and the Laboratory for antibiotics, enzymes, probiotics and starter cultures, Food Technology and Biotechnology, University of Zagreb).”

Prof. Dr. J. Tanzer, The University of Connecticut Health Center

“The laboratory tests conducted at the University of Connecticut Health Center demonstrated the ability of theL. paracasei used in pro-t-action™ to significantly reduce the levels of decay. “These findings, which are the logical consequence of the ability of selected L. paracasei to specifically clump Streptococcus mutans, present an intriguing new approach to controlling this miserable, prevalent, and costly disease, tooth decay.”

CONTACT

Neva MarketingAtlantic GrupaHealthCare DivisionMiramarska 2310000 ZagrebCroatia

E-mail: branimir.zoraja@atlanticgrupa.comPhone: + 385 91 2413 731

www.plidenta.com/enwww.facebook.com/plidenta.hrwww.youtube.com/ThePlidenta