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PLATELET RICH PLASMAPlasma Kaya Trombosit (PKT)

disusun untuk memenuhi tugas mata kuliah Biokimia Dasar

Pengampu : Edhana Sahiratmad!a" dr#" Ph#D

Program Magister Ilmu $edokteran Dasar

%akultas $edokteran &ni'ersitas Pad!ad!aran

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(hat is PRP)

Platelet Rich Plasma (PRP) isblood plasma that has been

enriched with platelets

PRP is simply whole blood that iscentrifuged to create an increasedconcentration of platelets with or

without WBCs

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Platelet Ri*h Plasma

frasi plasma darah dengan onsentrasitrombosit !"# ali di atas nilai normal(onsentrasi trombosit pada whole blood)

adar trombosit normal dalam darahberisar antara $#%&%%%' dan !#%&%%%'

plasma aya trombosit mengandung$&%%%&%%% trombosit' pada *olume # mlplasma

Plasma Kaya Trombosit (PKT)

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(hat+s So Spe*ial A,out Platelets)

+ platelets contain granules that store

large numbers of ,growth factors

+ alpha granules released (acti*ated)

when platelets aggregate and adhere

to a site of in-ury or inflammation+ listing of other growth factors.

interleuin"$/ tumor necrosis factor/

transforming growth factor/ insulin

growth factor and more

+ it is the a*ti'ation o- platelets andrelease o- the 'arious gro.th

-a*tors that makes them spe*ial

and is the ke to enhan*ing tissue

repair and healing/

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plasma aya trombosit mengandung 0 ma*am gro.th-a*tordan adar growth in i*o tetap ter!aga setelah

dilauan pembuatan plasma aya trombosit

konsentrasi trom,osit dalam PKT dapat meningkat12dari adar trombosit di dalam darah sehingga adar

gro.th -a*tor di dalam plasma aya trombosit -ugameningkat 12 ecuali 012"$

tingginya onsentrasi trombosit dan berbagai fatorpertumbuhan di dalamnya membuat PKT

diman-aatkan pada ,anak *a,ang ilmu kedokteran

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Platelet Ri*h Plasma Biolog

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(hat Do These 3ro.th %a*tors Do)

directional mo*ement in response to a chemical

stimulus

chemota3is

significant increase in cellular reproduction acti*ity

cell proliferation

performance enhancing

possibly e*en a systemic effect

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The Primar 3ro.th %a*tors Role

in So-t Tissue Healing and Repair

P412

5 polypeptide chains

6B/ BB and 66 types(6B in human platelets)

stored in alpha

granules in platelets receptors are found intendon/ cartilage/syno*ium andelsewhere

T12 beta

polypeptide/ multiplechains/ T12 beta"$most studied form

found in platelets/bone/ cartilage 7macrophages

receptors are uni*ersalin cells throughout thebody

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immediate

(within # minutes) second messenger stimulation

inflammatory response

early

(!%min to 8 hours) 9"R:6 stimulation/ protein synthesis chemota3is (draws cells to the area)

late (8"58 hours) fibroblast mitosis

E--e*t o- T3%45 on Tissues

actually inhibits cell growth

more of a modulator of cell growth/differentiation

*ery important in wound repair

E--e*t o- PD3% on Tissues

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E--e*t o- Platelet 3ro.th %a*tors on Tissues

it appears that through a comple3series of biochemical and cellular

e*ents

these growth factors cause a rapidand sustained increase in the number

of fibroblasts in an area and thenthrough a poorly understood

interaction among factors modulatescellular acti*ity

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PRP IS 67T/

;tem Cells9;Cs

ipocytes

Bone 9arrow