The Transpiration Stream How does water move into, through and out of a plant?
plant transpiration
-
Upload
bryan-atas -
Category
Documents
-
view
6.549 -
download
0
description
Transcript of plant transpiration
Plant transpiration
Introduction
Methodology
Summary and Conclusions
Results and discussion
Light and Photosynthesis
IntroductionMethodology
Summary and Conclusions
Results and discussion
Flowchart
Plant transpiration
Introduction
Methodology
Summary and Conclusions
Results and discussion
Light and Photosynthesis
IntroductionMethodology
Summary and Conclusions
Results and discussion
Flowchart
Plant TranspirationPlant Transpiration
Lateral MovementLateral Movement
And And
Path of Water RisePath of Water Rise
Plant transpiration
Introduction
Methodology
Light and Photosynthesis
IntroductionMethodology
Flowchart
Results and discussion
Summary and Conclusions
Results and discussion
Summary and Conclusions
DefinitionDefinition
TranspirationTranspiration
evaporation of water from plants. It occurs evaporation of water from plants. It occurs chiefly at the leaves while their stomata are chiefly at the leaves while their stomata are open for the passage of COopen for the passage of CO2 2 and Oand O22 during during
photosynthesis.photosynthesis.
Factors Affecting TranspirationFactors Affecting Transpiration
1.1. LightLight
2.2. HumidityHumidity
3.3. TemperatureTemperature
4.4. WindWind
5.5. Soil moistureSoil moisture
6.6. Internal Conc. Of COInternal Conc. Of CO22
lightlight
Plants transpire more rapidly in the light than Plants transpire more rapidly in the light than in the dark. in the dark.
light stimulates the opening of the stomata. light stimulates the opening of the stomata. Light warms the leaf. Light warms the leaf.
TemperatureTemperature
Plants transpire more rapidly at higher Plants transpire more rapidly at higher temperaturestemperatures
Temperature affects humidityTemperature affects humidity
HumidityHumidity
When the surrounding air is dry, diffusion of When the surrounding air is dry, diffusion of water out of the leaf goes on more rapidly. water out of the leaf goes on more rapidly.
Humidity is affected by temperatureHumidity is affected by temperature
WindWind
no breezeno breeze air is humid thus reducing the air is humid thus reducing the rate of transpirationrate of transpiration
breeze breeze humid air is carried away and humid air is carried away and replaced by drier air. replaced by drier air.
Internal Concentration of COInternal Concentration of CO22
If COIf CO2 2 concentration decreases concentration decreases stomata stomata
opensopens
Reverse is also trueReverse is also true
Underlying Principle of the Underlying Principle of the ExperimentExperiment
Rate of transpirationRate of transpiration = Rate of water absorption= Rate of water absorption
Transpiration decreases the water potential in the mesophyll cell, and thus, pulls water up from the roots and stems in vessels and xylems
XylemXylem
Consists of tracheids and vessel cellsConsists of tracheids and vessel cells Transport water Transport water Have pits at the side to allow lateral movementHave pits at the side to allow lateral movement
Plant transpiration
Introduction
Methodology
Summary and Conclusions
Light and Photosynthesis
IntroductionMethodology
Flowchart
Results and discussion
Summary and Conclusions
Results and discussion
Summary and Conclusions
MethodologyMethodology
Control set-upControl set-up Kept inside the room Kept inside the room
120 (botany lab)120 (botany lab) Away from electric Away from electric
fans and windowsfans and windows One branch covered One branch covered
with plasticwith plastic Held by iron clampsHeld by iron clamps Experiment lasts for Experiment lasts for
2 hours2 hours
Under bright light set-upUnder bright light set-up
Kept inside the Kept inside the room 120 (botany room 120 (botany lab)lab)
Away from Away from electric fans but electric fans but BESIDE THE BESIDE THE WINDOWSWINDOWS
Held by iron Held by iron clampsclamps
Experiment lasts Experiment lasts for 2 hoursfor 2 hours
Moving air set-upMoving air set-up
Kept inside the Kept inside the room 120 (botany room 120 (botany lab)lab)
BESIDE the BESIDE the electric fan but electric fan but away from the away from the windowswindows
Held by iron Held by iron clampsclamps
Experiment lasts Experiment lasts for 2 hoursfor 2 hours
Blocked stomata setupBlocked stomata setup
Kept inside the room 120 (botany lab)Kept inside the room 120 (botany lab) Away from the electric fan and from the Away from the electric fan and from the
windowswindows Held by iron clampsHeld by iron clamps Experiment lasts for 2 hoursExperiment lasts for 2 hours Stomata were blocked by Petroleum jellyStomata were blocked by Petroleum jelly
Lateral movement setupLateral movement setup
Lasts for 24 Lasts for 24 hourshours
Plant transpiration
Introduction
Methodology
Summary and Conclusions
Results and discussion
Light and Photosynthesis
IntroductionMethodology
Summary and Conclusions
Results and discussion
Flowchart
Results and DiscussionResults and DiscussionmL of HmL of H22O left O left
unabsorbed unabsorbed
ControlControl 3.23.2
Under strong sunlightUnder strong sunlight 1.51.5
Covered stomataCovered stomata 4.54.5
With Moving airWith Moving air 2.42.4
Versus controlVersus control
Plants absorb more water if exposed to strong Plants absorb more water if exposed to strong light and moving airlight and moving air
More water transpires from the leaves if the More water transpires from the leaves if the plant is exposed to strong sunlight or moving plant is exposed to strong sunlight or moving air air
Versus replicatesVersus replicates
Less water is absorbed if the stomata are Less water is absorbed if the stomata are blockedblocked
Less water is transpired when the stomata are Less water is transpired when the stomata are blockedblocked
Covered branch in ControlCovered branch in Control
Water droplets form at the walls of the plastic Water droplets form at the walls of the plastic
Begonia setupBegonia setup
A tube like structure was observed when begonia A tube like structure was observed when begonia absorbed the dyeabsorbed the dye
Plant transpiration
Introduction
Methodology
Summary and Conclusions
Results and discussion
Light and Photosynthesis
IntroductionMethodology
Summary and Conclusions
Results and discussion
Flowchart
Summary and ConclusionSummary and Conclusion
Plants transpire more if exposed to strong Plants transpire more if exposed to strong light and moving airlight and moving air
Plants transpire less if the stomata are blockedPlants transpire less if the stomata are blocked
ConclusionConclusion
Plants transpire more if the moisture content of air Plants transpire more if the moisture content of air (humidity) is less than the concentration of water (humidity) is less than the concentration of water inside the leavesinside the leaves
Humidity of air adjacent to the plant leaf (or stomata) Humidity of air adjacent to the plant leaf (or stomata) is inversely proportional to heat, light and windis inversely proportional to heat, light and wind
Plants transpire more if there is more heat, light, and Plants transpire more if there is more heat, light, and windwind
Presence of water dropletsPresence of water droplets
Presence of water droplets indicate that water Presence of water droplets indicate that water was released from the branchwas released from the branch
Specifically HSpecifically H220 was released from the stomata 0 was released from the stomata
and accumulated in the walls of the plasticand accumulated in the walls of the plastic
Begonia setupBegonia setup
Water travels through the xylemWater travels through the xylem
Xylary elements are hollow Xylary elements are hollow
Plant transpiration
Introduction
Methodology
Summary and Conclusions
Results and discussion
Light and Photosynthesis
IntroductionMethodology
Summary and Conclusions
Results and discussion
Flowchart
Light and PhotosynthesisLight and Photosynthesis
Plant transpiration
Introduction
Methodology
Summary and Conclusions
Results and discussion
Light and Photosynthesis
IntroductionMethodology
Summary and Conclusions
Results and discussion
Flowchart
PhotosynthesisPhotosynthesis
Uses photons to eject electrons from the trap Uses photons to eject electrons from the trap molecules in photosystems I and IImolecules in photosystems I and II
Thus, the presence of light affects Thus, the presence of light affects photosynthesisphotosynthesis
Plant transpiration
Introduction
Methodology
Summary and Conclusions
Results and discussion
Light and Photosynthesis
IntroductionMethodology
Summary and Conclusions
Results and discussion
Flowchart
MethodologyMethodology
Hydrilla setupHydrilla setup
Bubbles came out from the Bubbles came out from the shoot when the setup was shoot when the setup was exposed to bright lightexposed to bright light
Coleus setupColeus setup
Pigments being Pigments being extracted using 95% extracted using 95% ethanolethanol
Coleus leaf starts to Coleus leaf starts to lose its greenish colorlose its greenish color
Coleus setupColeus setup
IKI dropped to the coleus IKI dropped to the coleus samplesample
IKI is an indicator for the IKI is an indicator for the presence of starchpresence of starch
Coleus sampleColeus sample
Plant transpiration
Introduction
Methodology
Summary and Conclusions
Results and discussion
Light and Photosynthesis
IntroductionMethodology
Summary and Conclusions
Results and discussion
Flowchart
Results and DiscussionsResults and DiscussionsColeus setupColeus setup Bluish coloration on the Bluish coloration on the
uncovered leaf was uncovered leaf was greater compared to that greater compared to that of the covered leafof the covered leaf
Hydrilla setupHydrilla setup Bubbles were released Bubbles were released
upon exposure to upon exposure to sunlightsunlight
Hydrilla setupHydrilla setup
Time intervalTime interval Dim lightDim light Bright lightBright light
1 minutes1 minutes 00 2020
22 00 1717
33 00 1414
44 00 1212
55 00 1212
66 00 1010
77 00 1111
88 00 1414
99 00 1313
1010 00 1212
Hydrilla setupHydrilla setup
1 2 3 4 5 6 7 8 9 10
dim lightbright light
20
17
14
12 12
10 11
1413
12
0 0 0 0 0 0 0 0 0 0
02468
101214161820
minute interval
no. of bubbles per minute
no. of bubbles per minute interval
dim light bright light
Plant transpiration
Introduction
Methodology
Summary and Conclusions
Results and discussion
Light and Photosynthesis
IntroductionMethodology
Summary and Conclusions
Results and discussion
Flowchart
Hydrilla graphHydrilla graph
The number of bubbles seems to be The number of bubbles seems to be diminishing as time passes bydiminishing as time passes by
Dissolved CODissolved CO2 2 may have been used upmay have been used up
No evidence (bubbles) for the occurrence of No evidence (bubbles) for the occurrence of photosynthesis in dim lightphotosynthesis in dim light
Summary and ConclusionSummary and Conclusion
Light is needed in photosynthesisLight is needed in photosynthesis