Patterns of gene expression Drosophila embryogenesis in.
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Transcript of Patterns of gene expression Drosophila embryogenesis in.
Patterns of gene expression
Drosophila embryogenesis
in
RNA insitu and imaging pipeline
Richard Weiszmann
96-well plate RNA insitu
DGC1 and DGC2
Elaine Kwan
production snapshots
Amy Beaton
imaging and annotation
Status report
- to date we captured 25000 high resolution images
- we examined approximately 2500 genes
- images were captured for over 1200 genes
Correlation of microarray and image data
Novel insitu pattern – is it real ?
AnnotationCollaboration with Volker Hartenstein
differentiated organ
malpighian tubules
tracheal system
oenocytes fat body
anlage in statu nascendi
dorsal ectoderm mesoderm
procephalic ectoderm hindgut
primordium
malpighian tubules
posterior midgut
anlage
head mesoderm
large intestine
anlagein statu nascendi
anlage primordium differentiatedorgan
~ ~ ~
stage4-6
mesectodermanlagein statu nascendi
~
stage7-8
mesectodermanlage
~
stage9-12
midline primordium
~
Tracing back single-minded
stage13-16
midline glia
- allows integration with existing gene expression data
- annotation terms can be easily expanded for greater detail
Gene ontology
-Flybase controlled vocabulary for anatomy
(collaboration with Michael Ashburner).
- allows sophisticated searches of the dataset
http://www.fruitfly.org
Coming
soon
to
collaboration with Suzanna Lewis and Shu ShengQiang
Three adjacent Brachyury/T-Box genes at 66E4
CG7312 - earmuff (encodes a Zinc_Finger_C2H2, EF_hand protein)
Drosophila
Zebrafish XenopusYang, Z. et.al. Matsuo-Takasaki, M. et.al.
Acknowledgements
Sue CelnikerRichard Weiszmann
Amy BeatonElaine Kwan
Volker Hartenstein
Michael AshburnerSuzanna LewisShu ShengQiang
Gerry Rubin