Oncogene, anti-oncogene and growth factor The biochemistry and molecular biology department of CMU.
-
Upload
patience-stevens -
Category
Documents
-
view
235 -
download
2
Transcript of Oncogene, anti-oncogene and growth factor The biochemistry and molecular biology department of CMU.
oncogene, anti-oncogene and growth factor
The biochemistry and molecular biology department of CMU
Relationship of oncogene, anti-oncogene and growth factor
§1 Oncogene
• Oncogenes are cellular genes that can trigger uncontrolled cell proliferation if their sequence is altered or their expression is incorrectly regulated.
• A gene found in viruses or as part of th
e normal genome that is involved in trig
gering cancerous characteristics.
• Control the cell growth and cell differen
tiation.
• virus oncogene (v-onc) and cellular- o
ncogene (c-onc) or proto-oncogene
Virus- oncogene (v-onc)
• Genes are in viruses that can
cause tumors in vivo and
transform the cell in vitro.
In 1910, Peyton Rous found the first retrovirus in a chickens filtrated sarcoma. Rous got the Nobel Prize in Physiology and Medicine in 1966.
Cellular Oncogene (c-onc)
• Genes are in static or low-level
expression state in normal cells
under the normal situation and play
an important role in maintaining the
normal function of cells.
Category and major product of cellular oncogene
1. src family — tyrosine protein kinase
2. ras familiy — P21( GTPase activity)3. myc familiy — intranuclear DNA binding
protein
4. sis familiy — P28 (similar platelet derived growth factor)
5. myb familiy — intranuclear transcription factor
Product and Function of Proto-oncogene
• Extracellular growth factors
• Transmembrane growth factor receptors
• Intracellular signal transduction proteins
• Intranuclear transcription factors
Mechanisms of Oncogene Activation
• Obtaining promoter or enhancer
• Group translocation or chromosome rearrangements
• Proto-oncogene amplification
• Gene mutation
familiar human growth factor Factor Principal resource Primary function EGF
Submaxillary gland, kidney, duodenal gland
EPO
kidney
HGF
Placenta IFN- γ Activated TH1 and NK cells
IGFNGF
Placent, primarily liver, plasmNeur, submaxillary gland
PDGF Platelets,endothelial cells,placenta TGFα Tumor cell, transforming cell, placenta TGFβ Activated TH1 cells(T-helper) and
natural killer(NK) cells
VEGF Smooth muscle, tumor