Olympus BH2 BHTU - User Manual

8/13/2019 Olympus BH2 BHTU - User Manual

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MO EL HTUIOLOGIC L MICROSCOPE

scanned on Nov 6 2009 by J G McHonefor personal use only not for sale

. W RNINThis instruction m nu l s fo r us your instrumentBefore putting it into operation w recommend youread this manual c refully in order to famil iarize your-self fUlly with its us th t you m y o t in opti-mum performance

OLYMPUSAX ?


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This instruction manual has been writ ten for the use of the Olympus Biological MicroscopeModel BHTU It is recommended that you read the manual carefully in order familiarizeyourself fully with the use of the microscope, so that you wil l obtain optimum performance.

IMPORTANTObserve the following points: Operation

Always handle the microscope with the care it deserves, and avoid abrupt motions2 Avoid the use and maintenance of the microscope in direct sunlight, high temperature and

humidity dust and vibration3 Only use the tension adjustment ring fo r alter ing the tension of the coarse adjustment

knobs. Do not twist the two coarse adjustment knobs in opposite directions simultaneous-Iy, as this will cause damage.

4 Make sure that the voltage selector switch on the bottom base of the microscope standis set conform with the local mains voltage.5 Ground the microscope in case there is no ground terminal in your mains line .

Maintenance Lenses must always be kept clean. Carefully wipe off oi l or fingerprints deposited on the

lens surfaces with gauze moistened with a small amount of xylene, a lcohol or ether .2 Do no t use organic solutions to wipe the surfaces of various components Plastic parts,

especially, should be cleaned with neutral detergent.3 Never disassemble the microscope fo r repair. Only authorized Olympus service personnel

should make repairs.4 The microscope should be covered with the vinyl dust cover provided and stored in a place

free from humidity and fungi.


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IIIIII

STANDARD CONFIGURATIONSNOMENCLATURE SSEMBLy

ONT NTS

234

IV IDENTIFICATION AND FUNCTION OF VARIOUS COMPONENTS

VIVII.

OPERATIONA Switching ON the Light SourceIVoltage Adjustment and Light Intensity II LP Light Intensity Presetting Switch I

B Placement of a Specimen Slide . White over for ondenserI Cover Glass II Specimen Slide II Stage I

C Observation Tube Interpupillary Distance Adjustment2 Diopter Adjustment3 Light Path Selector

D Condenser Adjustment ondenser entrationIFiled Iris Diaphragm IIAperture Iris Diaphragm

E Focusing Adjustment . Tension of Coarse Adjustment Knobs and Fine Adjustment

IUse of Rubber Cap for Fine Adjustment Knob2 Pre-Focusing LeverF Use of Immersion ObjectivesG Photomicrography .OPTICAL DATATROUBLESHOOTING GUIDE

69

9

3

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ST ND RD ONFIGUR TIONS

ModelComponent BHTUll1 BHTU 2 BHTU3 2Microscope stand BHTUF 1 1with quintuple revolving nosepiece

8 inocular tube BH2B130 0Observation tubes Trinocular tube BH2TR30 0 0 Square mechanical stage with right-hand low BH2.SVR2 1 1 drive coaxial controls and specimen holderSwing out condenser BH2SC 1Halogen lamp holder LS20H 1 Halogen bulbs 6V2 WHAL 2 2 2D Ach. 4X D Ach. lOX D Ach, 4 X 0 0D Ach. lOOX oil eachObjectives D Plan 4X 0 Plan lOX D Plan 40X 0 D Plan lOOX oil each eachEyepieces paired WKlOX 1 1Photo eyepiece NFK3.3X 0 Filter KB4 1 1Immersion oil bottled 1 1 Vinyl dust cover 1 1

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II. NOMENCL TUREThe Model BHTU consists of various components and interchangeable accessories as shown inthe photo below. A wide v ri ty of combinations standard or optional, is available according your requirements.

Eyepiece

Objective

Stage

Condenser

-

Observation tube

Micoscope standor biological use

Halogen lampholder

P Light IntensityPresetting switch


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III. ASSEMBLYThis picture il lustrates the sequential procedure of assembly. The numbers Indicate theorder of assembly of various components Remove dust caps before mounting componentsTake care to keep all glass surfaces clean, and avoid : ;cratch ing the glass surface.NOTE: For numbers and please refer to explanations in detail on the next page.

e Condenser

The N.A. scaleengraved on th e c on -denser shou face themicroscope front. To AC outlet

Halogen bulbQ D Halogen lampJ holder

Condenser clamping screw

Observation tube

Observation tube clamping screwCircular dovetai l mount

Eyepjece0

ObjectiveSpecimenholder

Clampingscrews

~ t g L ; ~ ;Stage mounting dovetail Light Intensity Presetting switch


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xplanations in detai I Mounti ng the stage

Loosen the stage clamping screw CD byrotating counterclockwise. Fig.

2 Insert the st ge into the mounting dove-tail of the microscope stand slowly andlock with clamping screw.

f Mounting the observation tube1 Loosen the clamping knob CD fullyFig.21 Pull spring-loaded clamping knob

CD This will cause the locating pin


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Photo tube

Condenser heightadjustment knob

Pre focusi ng lever

Line cord

7

Diopter adjustmentring

X axis low drivecontrol knobX excursion range:7 mm

Y axis low drivecontrol knobY excursion range50mm

Field iris diaphragm ringArrow mark 0 0 indi catesincrease in diaphragm diameter


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Summary of Putting the Microscope into Operation MODEL BHTU

Match the voltage selector switch to local mains vol tage page 9 .B Sw itch on the light source page 9).C Place a specimen slide on the mechanical stage.O Coarse focus with the lOX objective.E Make interpupillary distance and diopter adjustments page 12F Adjust the condenser position page 13).G Swing in th e desired objective.H Adjust light intensityI Fine focus.J. Adjust aperture iris diaphragm and field j ris diaphragm ~ p g 13).

I Adjustment of Illumination System for Various Objective Powers Condenser

Objective Achromatic-aplanatic Abbe Swingout Low powermagnification condenser condenser condenser condenserBH2-AAC BH2-CD BH2-SC BH2-UL-C

2X Swi ng out top Compatible4X lens

lOX20X Compatible40X Compatible Swing in toplensBOX - - - - - - - - - - . -lOOX

N A is somewhat low, but still compatible with a 100X objective.

Take a copy of this page and put i t on the wall near the microscope for use as a reminder ofmicroscopic procedure.)

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v. OPERATIONA. Switching on the Light Source1 Ascertain that the voltage selector SWitch is set to conform WIth the local mains voltage.Fig. 3If the switch is not correctly set. adjust it ymeans of the Allen wrench provided, or ascrew-driver.

2 Place the sliding voltage control lever on thenght side of the microscope base to a lXlSitiondosest t o you low voltage position . Switchon the light source ma;n SWitch 2 1 . FIQ. 3 , .Fig. 3IVoltage Adjustment and Ugh Intensity As you push the control lever j) in the directionof the ARROW. the voltage increases. and theLED readout 2 \ill display the lamp voltage.Fig. 4l l ight Intensity Presening Switch

Apart from the light intensity set y voltageadjustment control lever, the LP switch can setthe l ight intensity as required.Presetting the light intensity Fig. 5

Switch ON the LP switch CD.2 Insert a slotted screwdriver into me LP j u s t ~

ment screw 1 . Turning it clockwise will in-crease the light intensity. Turn it until requiredintensity is obtained.* The light intensity wil l not change even

if the vol tage adjustment lever @ withthe LP switch ON. To change the lightintensity by using the voltage adjustmentlever, switch the LP switch OFF. To recallthe light intensi ty preset, push the LPswitch to ON.

A Hint on Convenient Applicat ion of the LPSwitchFor instance, when you want to use two kinds ofobjective lens ahernatley. set one objective by usingthe vohage adjustment [ever. then set anotherobjective lens by using the LP switch. This allowsthe ffght intensity 10 recallhe intensity initially presetby switching the LP swi lch ON and OFF.This also allows the light intensity setting forphotomicrography.

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Fig. 4

Fig. 5


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Fig. 6

B. Placement of a pecimen Slide Rotate the coarse adjustment knobs in the

direction of the ARROW to rack down the stageso that a specimen slide can be placed on thestage. Fig. 6NOTE: The rotation of the coarse and fine

adjustment knobs in the direction of theARROW will rack down the stage.

2 Opening the spring-loaded finger of the specimen holder with one hand place a specimenslide Inside the holder with the other hand.When the slide comes in contact with the backof the specimen holder slowly return the springloaded finger.WARNING: If the spring-loaded finger IS re-

turned t.oo quickly it may causedamage to the specimen slide.3 For use of the specimen holder B2-HL2

A specimen slide can be easily slipped into thespecimen holder by single hand operation. Fig.7

Fig. 7White Cover for ondenserThe white cover for condenser should becovered over the condenser. Fig. 8

When setting stain specimens under thespecimen holder the white cover for condensercomes into field of view as a background. Thisallows easy distinction what you are observingbetween its background.

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ICover Glass I An Olympus objective engraved 16010 17 requires a ov r glass of 0 17mm thickness.

If the numerical aperture of the objective is 0.7 or higher except immersion objectives)and no cor rect ion col la r is provided, the resolving power deter iorates great ly, if ov rglass thickness deviates from the above listed value.NOTE: In some countries a 0.17mm thick cover glass corresponds to a 1 Y, cover glass.

A cover glass 0.4 mm thick fo r blood counting, etc. can be used with Olympus objectivesexcept 0 Plan 40X S Plan Apo 40X and S Plan lOOX.

ISpecimen Slide I Specimen slides 0.8 mm to 1.5 mm thick are

recommended for Olympus objectives. Specimen slides 0.8 mm to 1.2 mm thick are

recommended for, the darkfield condenserand the dj fferential interference contrastdenser.

3) Bring the portion of the specimen to beobserved into the l ight path by means of thelow drive stage control knobs. Fig. 9)* Tighten the stage clamping screw .

IStage I The specimen holder can accommodate two

standard specimen slides simultaneously. The specimen holder is removalbe to obtain

a large unobst ructed stage surface to holdspecimens up to 55 mm x 85 mm.

To rotate the stage loosen the tage clampingscrew and holding this screw, ro ta te thestage into the desired direciton. Fig. 10)

Stage clips fo r use with immersion objectives.Fig. 111A pair of stage clips is optionally availableto ho ld the specimen on the stage, elimina-ing specimen drag or buckling due immer-sion oi l between slide and stage surface. ThecI ips can be inserted into the holes provided on the specimen holder.

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Fig 10

Fig. 11


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c Observation Tube1 Interpupillary Distance Adjustment

1} Click the lOX objective into position.2 Looking through the eyepieces with both

eyes, adjust the interpupillary distance ofthe binocular tube by adjust ing theknurled dovetail slides of the right andleft eyepiece tubes with b9th hands untilperfect binocular vision is obtained. {Fig.121

-Fig. 12

2 Diopter Adjustment Looking at the image through the right

eyepiece with your right eye, focus onthe specimen with the f ine adjustmentknobs.

21 Next, looking at the image through theleft eyepiece with your left eye, rotatethe diopter adjustment ring to focuson the specimen without using thecoarSE and fine adjustment knobs..(Fig. 131

3 Light Path Selection1 The trinocular tube is provided with a

light path selector lever CD to d irect thelight to the observation tube and/or tothe photo tube in 3 positions. (Fig. 141

Indicator Plate

11tii -:t

,1lIII\ _ f ~.:.

Fig. 14

CDrLever Position

Pushed in all the way Pulled out: halfway Pulled ou t all the wayVI (C. 11 1 CAmount 100 into binocular tube 20 into binocular tube 100 into photo tubeof light 80 into photo tube

Observation CD Observation of exces- Photom icrography ofApplica sively b r i ~ l h t specimens dark specimensV Dark specimens Photomicrography fotion cusing through the bi-nocular tubeAn indicator plate is prov ided at the knob port to summarize the usage of the above table;it can be consulted before operating the knob

V: Viewer white letters)CV: Camera viewer (yellow-green letters)C: Camera (red letters)

The colors of the letters correspond wi th the color bands on the knob shaft.

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D Condenser Adjustment

Fig. 14

Fig. 15

1. Condenser Centration1) Stop down the field iris diaphragm with

knurled ring by rotating in the direc-tion of the arrow. Fig. 14)

2) Use the condenser heigh t adjus tmentknob to move the condenser up anddown until an image of the field dia-phragm can be seen clearly in the eye-pieces. The rotation of the knob in thedirection of the arrow lowers the con-denser.

Field irisdiaphragm image Field of view

3) Bring the field iris diaphragm image into the center of the field of view with the tw o con-denser centering knobs . Fig. 14)

4 Widen the diameter of the iris diaphragm progressively. If the polygonal image of the irisdiaphragm becomes inscribed in the field it means that the field diaphragm is centered.Fig. 15

Field Iris Diaphragm I The field iris diaphragm controls the diameter of the ray bundle impining on the specimen surface and therefore by stopping down the f ie ld d iaphragm until it is slightly

larger than the field of view it can reduce stray light which in turn increases image defi-nition and contrast.

70 8020 - 30 ,

Fig. 16

Opening of theaperture diaphragmObjective exit pupil

Aperture Iris Diaphragm I In order to achieve opTimum objective performance the opening of the aperture iris

diaphragm should be matched to the numerical aperture of the objective in use It isoften preferable however to stop down the aperture diaphragm slightly more than in-dicated by the objective N A This will result in better image contrast increased depth offocus and a flatter field.

After complet ing focus adjustment re-move one of the eyepieces from the ob-servation tube and look into the emptyeyepiece tube. As you stop down theaperture iris diaphragm the image of theiris diaphragm can e seen in the object-ive pupil. Adjust the opening of thediaphragm to match the N.A. of the ob-jective in use If the specimen is low incontrast it is recommended to stop downto 7 8 of the objective N.A. Fig.

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E Focus Adjustment

Fig. 18

Adjustment Knobs andAlthough the tension of the coarse adjustment knobs has been already adjusted foroptimum performance by the manufacturer.it possible to personally adjust the tensionof the coarse adjustment for either heavy orlight movement depending on the operator spreference by rotating the tension adjustment ring D Fig. 181The ring can be rotated by inserting a screwdriver into one of the holes on the periphery thering. The clockwise rotation in the direction of the ARROW tightens the coarse adjustmentknobs. Do not loosen the ring too much because the stage may drop or the fine adjustmentknobs may slip.

CD Tension of CoarseFine Adjustment.

NOT Do not rotate the right and left coarse adjustment knobs in the opposite directionssimultaneously. If the stage drops and the specimen goes out of focus. the tensionadjustment ring is too loose Tighten the ring

Use of Rubber Cap for Fine Adjustment Knob,Attaching this cap over the fine adjustmentknob increases the sensitivity of the fine focusing motion. The rubber cap optionallyavailable.

2 Pre-Focusing LeverThis lever ill is provided to prevent possiblecontact between specimen and objective aswell as simplify coarse focusing. Fig. 191The lever is locked after coarse focus hasbeen accomplished. This prevents furtherupward travel of the stage by means of thecoarse adjustment knobs, and automaticallyprovides a limiting stop if the stage is loweredand then raised again The pre-focusing leverdoes not restrict fine focusing Fig. 19


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F. Use of Immersion Objectives1 Focus the specimen with a low power objective.2 Put a drop of immersion oi l on the specimen slide and the front lens of the immersion

objective.3 Rotate the revolving nosepiece to bring the immersion objective into the l ight path andfocus with the fine adjustment knobs.

NOTE: ill For immersion condensers such as an achromatic-aplanatic condenser or Abbecondenser remove the specimen from the mechanical stage and place a drop ofimmersion oi l on the front lens of the condenser. Then place the specimen onthe stage and slowly raise the condenser until firm contact with the underside ofthe specimen slide is made. Care should be taken to prevent oi l bubbles from forming in the oi l film betweencondenser and specimen slide. If any bubbles appear re-apply immersion oilfo r these bubbles greatly deteriorate the lens performance.

After use. carefully wipe off the immersion oi l deposited on the lens surfaceswith gauze moistened with xylene. Never leave oil on the lens surfaces after useas oi l remnants will seriously impair the perforrrance of the lens system.

G. PhotomicrographyThe Olympus Photomicrographic Equipment Model PM lOAO is uniquely quali fied to beused with t he BHTU microscope for routine and advanced photomicrography. A separatedetailed instruction manual is available for the PM lOAD camera system.For quick reference however you may want to refer to the following pointers when usingthe PM-lOAD.

Photographic EyepieceUse NFK photo eyepieces fo r photomicro-graphy.Insert the eyepiece into the eyepiece tubeof the photo tube. Fig. 20

Fig. 202. Mounting the Photographic Unit

Slip the body of the photographic unit over thephoto tube. Align the dots on photo tube andthe PM- lOAD body and clamp the camera unitto the photo tube. Fig. 211

3. Setting the Light Path SelectorRefer section C.3. on page 12.

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Fig. 22

4. Focusing ProcedureUse the f ie ld of view eyepieces fo r focusing on the film plane. Each f ie ld of view eyepiecehas a focusing front lens and a relide with 4 frames each frame indicating the area coveredby a specific power NFK photo eyepeice. Fig. 22The number at each frame indicates themagnification of the photo eyepiece. Theimage in the field of view eyepiece and theimage on the film plane are in focus at thesame t ime. Several type field of view eye-pieces are available according to the filmsize employed.

Field of view eyepiece 35WHK10X PWHK10X 4X5WHK10X MHWHK10X35mm Back 3 x 4 4 x 5 16mm Bolex

Attachment camera Polaroid Sheet Film or cameraBack Polaroid Film 120 Roll F ilmHolder Holder

1 Select the f ie ld of view eyepiece matching the camera bSlck in use and insert it into ther ight eyepiece tube of the trinocular tube al igning locating groove and locating pin.

2 While looking through the field of view eyepiece rotate the eyepiece front lens in screwmount t focus on the double cross lines in the field. For sharp focusing with objectives4X or lower, the focusing magnifier FT is recommended on account of their considerabledepth of focus.

3 Bring the specimen detail t be photographed within the frame corresponding to thepower of the NFK eyepiece in use and focus on the specimen with the microscope fineadjus tment knobs. Make sure the light path selector knob on the observation tube iseither on the whi te (V) yellowijreen (eVI band.

4 It is recommended to tighten the tension adjustment ring considerably to prevent thestage from dropping during long exposures.

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V OPTICAL D TObjective Type o Achromat o Plan Ach.

Magnifi 4X lOX 40X l00X 4X lOX 4 X 100XcationN A 0 10 0 25 0 65 1 30 0 10 0 25 0 65 1 25

W.O. mm 18 2 7 2 0 6 0 20 7 03 7 4 27 0 17Focal length 30 03 16 9 4 58 1 91 34 23 17 5 4 67 1 75lmml

Eyepiece Resolving 3 36 1 34 0 52 0 26 3 36 1 34 52 0 27power jJ

WK10X Total mag. 4 X 1 X 400X 1000X 40X 1 X 400X lQ XField Focat 171 6 27 45 3 0 0 7 171 6 27 45 3 0 0 7number depth {jJJ2 1 Field of 5 2 0 5 0 2 5 2 5 0 2view mm

ll Immersion objectivesThe resolving power and focal depth are obtained witt fully opened aperture diaphragm

Technical terms Working distance: The distance from the cover glass to the nearest point of the

objective. Numerical aperture: The N.A. represents a performance number which can be com-

pared to the relative aperture f-number of a camera lens.N.A. values can be used for directly comparing the resolvingpowers of all types of objectives The larger the N the higherresolving power. Resolving power: The ability of a lens to register small details. The resolving powerof a lens is measured by its ability to separate two points.

Focal depth: The distance between the upper and lower limitsof sharpness inthe image formed by an optical system. s yo u stop down theaperture iris diaphragm, the focal depth becomes larger. Thelarger the N.A. of an objective the shallower the focal depth.

Field number: A number that represents the diameter in mm of the image ofthe field diaphragm that is formed by the lens in front of it

Field of view diameter: The actual size of the field of view in mm on the object surface.

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VII. TROUBLESHOOTING GUIDE yo u are unable to obtain full performance f ro m y ou r microscope please consult with th etable below as pointers fo r troubleshooting.

Phenomenon Cause emedy Optical Systema With i l luminator switched Field iris diaphragm IS no t Open diaphragm to proper

on the field of view is opened sufficiently. diameterdark. Condenser is lowered to o Adjust condenser height.

much.Light path selector lever is Push in lever up to CV or Vpulled ou t to C position. position.

b Field of view is cu t o ff or Light path selector lever is Click it into proper posit ionilluminated irregularly. stopped midway according to your purpose.

Nosepiece is no t clicked into Slightly rotate nosepiece untilplace it cl icks into place.Th e power of objective used Choose a condenser to meetexceeds th e illu mination ca- your purpose.pacity of condenser.Condenser is no t centered. Center condenser.Field irisdjaphragm is stopped Open diaphragm to properdown excessively. diameter.

c Dust or dirt is visible n Dust etc. on light exit lens Remove dust etc.th e field of view. Dust on condenser to p lens Clean front tenses

Dirty specimenDust on eyepiece

d Excessive image contrast Condenser is lowered to o Adjust condenser height.much.Aperture i ris diaphragm is Open diaphragm to properstopped down excessively. diameter.

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Olympus BH2 BHTU - User Manual

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