Nghiên Cứu Kháng Sinh Được Sinh Tổng Hợp Từ Streptomyces 183.221

7/21/2019 Nghin Cu Khng Sinh c Sinh Tng Hp T Streptomyces 183.221

1/65

J _ V

VZMA O NB FB N MO

V VN HN\_M

MANOM B\ HNMA ROMN B

ROMN VMA NP VRVZLPVCE_BLR

4>2.::4

HND I\M VV MANOP FB R

N MO- :04


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J _ V

VZMA O NB FB N MO

V VN HN\_M

MANOM B\ HNMA ROMN B

ROMN VMA NP VRVZLPVCE_BLR

4>2.::4

HND I\M VV MANOP FB R

Mao nma fm; PAR.VR Bdc Wm Vnu

Mo tnb nom; J em Wo somn - Romn nb

N MO :046


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IO BE M

Vrb tom, le xom ao io be m bnm tnmn su sb to tny aoc, PAR.VR

Bdc Wm Vnu- mao tm tmn nma fm le t mnma may u tom manom buhncd nb to hno ncm tnmn hnd ium tt manop.

Le xom bnm tnmn be m bb tny b aoc, bb bm j h tnut vom aoma

fy bma tb to J em Wo somn- Romn nb, J em bma manop Fb trma o

nb Fb N Mo8 J em Nd vt iou- Hncd Nd nb trma o nb Hncd nb

t mnom N Mo8 Wom v somn fbn t Vruma ma, Wom Nd nb tnub Wom Nm

ie Hncd nb v Bma man Wot Mde tc ou hom v aop le trcma qu

trmn tnb nom hnd ium.

Le bma xom bnm tnmn be m Jdm aoe nou bma tcm tn bb Vny B

aoc, bm j, vom bnb trma o nb Fb N Mo fy f v tc ou hom

tnum io bnc le trcma qu trmn nb tp to trma.

Buo bma to xom bnm tnmn be m to aod mn, jm j qudm te, ma

vom, aop to trcma sut tno aodm nb tp bma mn tnb nom hnd ium my.

Fc tno aodm ie tnb manoe bma mn hom tnb bd jm tnm b nm, hnd

ium my bm b mnou tnou st. Le rt ecma mnm b s ma ap hom t tny

b v jm j hnd ium b ncm tnom nm.

Le xom bnm tnmn be m!

N Mo, tnma 0< me :046.

Romn vom

V VN HN\_M


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EB IB

V WM .............................................................................................................. 4

BNMA 4; VMA T\DM ........................................................................................ :

4.4. O BMA W HNMA ROMN ................................................................... :

4.4.4. mn mand hnma somn .............................................................................. :

4.4.:. Pnm ico hnma somn ................................................................................. :

4.4.2. B bn tb fma bd hnma somn .................................................................. 2

4.4.6 . ma fma bd hnma somn ........................................................................... 2

4.:. B OE BD ^ HN\M (Dbtomceybltls) ............................................ 6

4.:.4. b oe nmn tno v hbn tnb ................................................................ 6

4.:.:. b oe bu tc bd t jc x hnum ......................................................... 6

4.:.2. b oe bd x hnum bnoRtrlptceybls.................................................... . Pnma pnp bnot hnma somn t fbn ib jma fuma eo nu b ............... :6

:.:.?. Pnma pnp xb mn hnma somn mo jc, maco jc ................................ :6

:.:.40. Pnma pnp xb omn jm mnot, jm pN bd hnma somn ................ :6

:.:.44. Pnma pnp tbn hnma somn jma sb h ............................................... :2.::4................ :1

2.:. HN MMA ROMN VMA NP HNMA ROMN BD RVZLPVCE_BLR

4>2.::4 VZM EV PNM IP- EV : ................................................................... :>2.2. HV T\ BNM EO VZMA M\O B_ .............................................. :>

2.6. HV T\ BO VC AOMA ......................................................................... :?

2.6.4. Ht qu bnm ib mau mnom ..................................................................... :?

2.6.:. Ht qu t jom ......................................................................................... 20

2.


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VO IO\ VNDE HNC .......................................................................................... 62


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FDMN EB BB H NO\, BB BN BO WOV VV

DFM Dbof :-flcxyrojcmubilob

BQ Vnmn t jc - Blii wdiiF ma hmn truma jmn bd vma v hnum, v me

FF Fuma fbn

FENB Fuma eo nu b

A(+) Arde fma

A(-) Arde e

OZ Omgrdrlf ( nma maco )

ORP Omtlrmdtocmdi RtrlptceyblsPrcklbt ( Bnma trmn RtrlptceyblsTubt )

HR Hnma somn

I-FDP I fodeomcpoelidt

ER Edss Rplbtrceltry (pn hno )

EV Eo trma

EVft Eo trma fbn tn

MRV Mnoe sb tnZG Zlbtogilxojoil ( tnma no bcma)

s Rdo s tnb manoe bnum nou bnmn

RHIE Rb h ip ema

\W \trd Wocilt ( t maco )

WRW Wo somn vt

W Vn tbn

WI Wo imaJ. sujtoios Jdboiius sujtoios

P. eordjoios Prctlus eordjoios


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FDMN EB BB JMA

Jma 4. 4. Et s hnma somn b maum ab t x hnum 1

Jma :. 4. Bb bnma vo hnum hoe mn 42.::4trm EV:. :>

Jma 2. 2. Ht qu id bnm EV muo by :>

Jma 2. 6. Ht qu bnm ib mau mnom. :?

Jma 2. . jm vma bd hnma somn vo mnot 22

Jma 2. ?. mn nma bd pN to jm vma hnma somn 22

Jma 2. 40. Ht qu id bnm fuma eo v pN bnot hnma somn. 26

Jma 2. 44. Bnot hnma somn jma Ltnyidbltdt pN5 1, bnot ip : im 2

Jma 2.41. Ht qu bny bt im 2 2?

Jma 2. 4>. Ht qu Zgbd bb bnt tnu b 2?

Jma 2. 4?. Hno ima v nou sut ht tomn 2?


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FDMN EB BB NMN

Nmn 2.4; Ht qu bnm EV im elm.

Nmn P4; Bb ico hnum ty x hnum.

Nmn P:; R pnt trom bd hnum ty x hnum.

Nmn P2; ma bcma somn trma v pnt trom bd vo hnum.

Nmn P6; Nmn mn bnuo jc t Rtrlptceybls 4>2.::4.

Nmn P2.::4.

Nmn P7; Ht qu t jom im 4.

Nmn P1; Nmn mn hnum ib x hnum sdu t jom :.Nmn P>; Ht qu id bnm EVft im elm.

Nmn P?; Nmn mn jmn mnm aoma bp 4 trcma im elm bnm bnma.

Nmn P40; Ht qu tn nct tmn bb pnm cm bny bt sb h im 4.

Nmn P44; Ht qu tn nct tmn bb pnm cm bny bt sb h im :.

Nmn P4:; tn tn nom mn nma bd pN m jm vma bd hnma somn.

Nmn P42; Pn \W.

Nmn P46; Pn hno.NmnP4


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4

V WM

Me 4?:>, Dilxdmflr Gileoma pnt nom rd hn mma hnma hnum bd me

Plmoboioue mctdtue, e u bnc manom bu v s fma hnma somn. Me 4?2>,

Gicrly v Bndom tnb manoe plmoboiiom trcma ou tr.

Vn trma bnt hnma somn trm tn aoo pnt trom v tma emn trcma mnma

me am y bnc tny toe mma tc im bd mamn bma man hnma somn trcma mm

homn t qub fm. ma tno s xut nom may bma mnou bb vo hnum d hnma

tnub v s tnou nt bb mne hnma somn eo, ie bnc bnma td dma pno o et

vo h mauym nu hnma somn (pcst dmtojoctob lrd). Bnmn v tn mnoe v t

rd bd mamn bma manop sm xut hnma somn i; et et bo jom bb hnma somn

b trmn tmn trma hnma tnub, et hnb pno tnb y manom bu v pnt trom

(Z&F) te rd bb bnt hnma somn eo vo bb b bn tb ma eo ncm tcm. W

vy Bma manop hnma somn tnu nt b s qudm te bd bb mn hncd nb

tnub mnou imn vb hnb mndu trm tn aoo.

ap pnm vc bma bub te rd bb bnt hnma somn eo, to bnm to ;

Manom bu hnma somn b somn tma np t Rtrlptceybls 4>2.::4vo eb tou;

Pnm ico mn tm hncd nb Rtrlptceybls 4>2.::4 tnlc ORP.

Bo tc aoma mma bdc hn mma tma np HR bd Rtrlptceybls4>2.::4.

Manom bu im elm, bnot xut tomn bn HR fc Rtrlptceybls4>2.::4 tma

np.

R j xb mn et s tmn bnt bd hnma somn tomn bn.


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:

BNMA 4; VMA T\DM

4.4.O BMA W HNMA ROMN

4.4.4.

mn mand hnma somn X4U, X6U X1UB mnou mn mand hnb mndu v HR. Nom mdy, aoo y nb qudm moe rma;

HR i mnma bnt tc tnmn fc bnuym nd somn nb, b tb fma he ne s pnt

trom bd vo hnum (jdbtlrocstdtob) ncb tou fot vo hnum (jdbtlrobofdi) mma

tnp, b sm xut jma somn tma np ncb tma np tnlc eu bb HR t mnom.

4.4.:. Pnm ico hnma somn X4U, X6U, X1U, X4:U

d. Fd vc tmn mny be bd vo hnum vo hnma somn

B tn pnm bb HR tnmn; HR fot hnum, HR he hnum

j. Fd vc b bn tb fma bd hnma somn

Fd vc b bn tb fma, bnod tnmn bb mne;

Vnub b bn tma np vbn t jc vo hnum; idbtde, vdmbceybom,

Vnub b bn ncb tndy o tma np prctlom bd WH; bicrdepnlmobci,

tltrdbybiiom, edbrciof,

Vnub b bn tma np dbof mnm; quomcicm, rogdepobom.

Vnub b bn bnuym nc; Bc-troecxdzci

Vnub ie tndy o tmn tne bd ema t jc; pciyeybom, depnctlrobom.

b. Fd vc bu trb nd nb

Fd vc bu trb nd nb, hnma somn bnod tnmn bb mne bnmn sdu;

Mne idbtde

- Plmoboiiom; Jlmzyiplmoboiiom, cxdboiom, depoboiiom

- Blpndicspcrom; blpndilxom, blgctdxoe, blgdbicr,

- Bb idbtde hnb; bdrjdplmle, bnt b bn idbtdedsl.

Mne deomcaiybcsof ( deomcsof ); strlptceybom, almtdeobom

Mne edbrciof; lrytnrceybom, bidrotnrceybom,

Mne iombcsdeof; iombceybom, biomfdeybom,

Mne pnlmobci; bicrdepnlmobci, tnodepnlmobci,


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2

Mne tltrdbybiiom; tltrdbybiom, fcxybybiom,

Mne plptof;

- Aiubcplptof; Wdmbceybom.

- Pciyplptof; pciyeybom, jdbotrdbom.

Mne quomcicm; dbof mdiofoxob, boprcgicxdbom,

Mne bc-troecxdzci; Bc-troecxdzci.

Maco rd HR b tn b pnm ico tnlc fb ma nb fb ib nb v

tnlc maum ab.

4.4.2. B bn tb fma bd hnma somnX4U, X6U, X


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6

Vrcma trma trt

Wob s fma HR jc v tnb vt le io nou qu homn t bdc, hnb pnb

b mnb oe bd bb tnub nd nb ( nom tma hnma tnub bd su jmn,

mnoe EV, bnt b tm f trcma sm pne b tn ay b bnc bcm mao,).

4.4.6.2. Vrcma bma manop tnb pne

Et s HR i mnma bnt jc qum tnb pne to v ma np i tma,

mn; mosom, sujtoiom, bictltrdbybiom,vo u oe; Vno aodm hn trma jma mnot

mam o, mnot hn trma aoe. Mma HR rt tnp, jc qum b iu fo, bnt

ima tt nm, votdeom hnma j pn ny, nma v t j jom o.

4.:. B OE BD ^ HN\M(Dbtomceybltls) X


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Bb WRW b s t jom t mnom vo tm s hncma 40-40 40-


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?

mn sma tnma; Rdu hno t jom jma mn sma \W, nm fbn jc t

b ym trcma jma to, mu le bnou mn sma tnma (5 2:0-6>0me), b

hncma 0% t jc b pnb no, et o tmn t jom fc tb fma bd lmzyel

pnctciydsl. Nom tma my ao i nom tma qudma pnb nct.

4.2.2. Jc qum aoma

Eb tou; Jc qum WRW et bbn hncd nb, ma h tnut ao

b qum tn bb t jc aoma sma st vo t i bdc, m mn v ao b bb b

tmn jdm u, hnma j tp mnoe jo bb WRW i.

Bb pnma pnp jc qum; Pnma pnp jc qum imn8 ao aoma trm

eo trma t, bt v trm nt ma bb8 pnma pnp imn su8 pnma pnp ma

hn.

ao aoma, jc qum x hnum trcma pnma tn manoe b tn s fma

pnma pnp trm eo trma tnbn manoma trcma t imn :0B.

4.6. IM ELM ROMN VMA NP HNMA ROMN

4.6.4. Hno moe im elm X46U

Im elm i qu trmn muo by WRW trcma mnma ou hom mnt mn mne

tbn iy bb sm pne trdc o bnt b bn t bnmn bb WRW ncb t bb tnmn pnm

t jc.4.6.:. Pnma pnp im elm X42U, X46U

D. Im elm j et

WRW b muo by trm j et fbn tn ncb jm rm. WRW np tnu bb bnt

fomn fma bd EV v s fma cxy hnma hn n np trm j et EV fomn

fma.

u oe; m aom, f tnb nom, u t trdma tnot j tnp.

Mnb oe; Nou sut s fma tnp, hn ao v trma, hn b aoo nd, tma nd, tm fom tbn, mnm bma.

J. Pnma pnp im elm bne

Pnma pnp im elm bne i hou im elm e WRW pnt trom trcma hnma

aodm 2 bnou bd EV ima.


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40

u oe; R fma EV fomn fma to u, p ma mnu bu somn i v fomn

fma bd WRW8 nou sut qu trmn im elm bdc8 ao v trma, f hoe sct tcm

j, f b aoo nd, t ma nd, tm t fom tbn, bno pn mnm ib tnp.

Mnb oe; Homn pn bnc trdma tnot j im ( trdma tnot j bnuym fma,

bnu p ib, ou hom v trma tuyt o), bm bm j bnuym em nd, b homn

manoe. Hnma tn x i bb j.

Bb hou im elm bne;

Im elm e ( aom cm) ; Vn tbn EV muo by hnma tndy o

trcma sut qu trmn im elm. Vnu sm pne buo qu trmn. Bb ou hom im elm

b e jc, WRW pnt trom tnlc 6 pnd; pnd toe tma, pnd ica ( iy tnd), pnd

fma (pnd bm jma), pnd suy tm ( Nmn P2- Pn ib).

Im elm b j suma; J suma EV muo by trcma qu trmn im

elm, mm tn tbn EV muo by tndy o.

Im elm iom tb; Iom tb buma bp bnt fomn fma v tnu sm

pne, mm tn tbn EV muo by hnma tndy o b tn bco i bm jma ma. Hno

e jc bb ou hom, WRW s hc fo pnd fma.

Im elm jm iom tb; mn h j suma bnt fomn fma v mn

h tnu sm pne.

4.


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44

bnuym t pnd my sdma pnd hnb. Vnb t tnma fma bm jma mb - fuma eo

nu b hnma tdm trcma mb ncb bm jma ima rm tbn v tomn bn HR.

Mauym tb; Fd vc s pnm j bnt tdm aod ndo pnd hnma ma tdm vo

mndu.

Bb pnma pnp bnot ima ima;

Bnot 4 im (bnot m); m aom, tm t tno aodm v fuma eo.

Bnot mnou im (bnot ip); Nou sut bnot bdc nm, mnma tm tno aodm,

bma sb, fuma eo.

Bnot mab fma; Mauym tb b jm i fuma eo bnot v eu bnot o

mab fma mndu. H tnut bnot mab fma bnc ht qu tt nm, tnma b p

fma trcma bma manop tbn bb bnt.

4.


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4:

bnou vc ano mnm eu ncb nom nmn jma bb tnub tn b nou; Momnyfrom,

WRW,

Rb h bt;

Pnd tmn; i bt bnd bnt mno mn; Roiobdali, bliiuicsl, mne cxyf,

Pnd ma; fuma eo m ncb nm np, tnma s fma nm np fuma eo

ie tma sb rd aoo. Pnd ma fo bnuym qud pnd tmn mn p sut ncb trma ib.

4.7. R J MANOM B\ B\ VZB HNMA ROMN

4.7.4. Pn nma maco X:U

Pn nma maco i ma bcma jou fom s pn tnub bma np tn jb

x nma maco bd bnt bm manom bu bb s sma hnb mndu.

4.7.:.

Pn \W-WOR X:U

Pn \WSWOR i ma bcma jou fom s pn tnub np tn jb x \W-

WOR vc jb sma ndy s sma.

4.7.2. Pn hno X:U

Hno pn i h tnut c trb top t s hno ima v om tbn bd ocm (e/z)

b tc tnmn trcma pnd hn t pnm t ncb mauym t bd eu.

Bb ocm b tc tnmn trcma juma ocm nd, b aod tb v tbn roma mn j

pnm pnm tbn hno trb hno m fltlbtcr. Vm nou tma ma vo bb ocm btn nom jma et s vbn (pob) b bma hnb mndu tp np io tnmn pn

hno (ER).

4.1.

Et s manom bu am y iom qudm m hnma somn, Rtrlptceybls

sp.

4.1.4. Nct tmn hnma hnum bd bb sm pne bnuym nd bd

Rtrlptceybls DP-4:2 v nou ma b t jc. X41U

Rtrlptceybls DP-4:2b fma so, b maum ab t hnu vb jom bd DmfrdPrdflsn, m . Rtrlptceybls DP-4:2b nct tmn hnma hnum bdc o vo tt b

bb WH tn manoe sc vo bb bnma Rtrlptceyblshnb.R b et bd aiybom v

dbof I-fodeomcpoeliob trcma tnmn t jc bn rd bb bnma tnub v bno

Rtrlptceybls sp. Aoo trmn t alm 47R rDFMbd Rtrlptceybls DP-4:2 bnc tny


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42

??% trmn t tma ma vo Rtrlptceyblsgidvcaroslus.Bb bnt HR b bnot

xut jma nlxdm v ltnyidbltdt.

Et np bnt tnu b jma bbn bnot xut s fma fu tn vo bb mma

hnb mndu bd fuma eo sdu b tomn bn jma sb h.Np bnt tn nom

nct tmn hnma hnum bdc o vo WH Ar (+), Ar (-), ma tno tnb manoe bnc

tny hn mma ay b t jc emn vo bb fma t jc b tmn- t jcWlrc ( tnm

hn xdmn ) v NLP: (t jc uma tn tndmn qum) trcma ma manoe. Mma b bn

to tnou (EOB) bd np bnt bnma io WHJ. sujtoiosv R. durlus tma ma i :

BdBC2 :,0 0,04

MdBi 4,0 0, -

1,0

Bb EV trm b tot hnum 44>-4:00B/ 20 pnt. Bb maum ma; F-

aiubcsl8 F- Edmmotci8 Zdggomcsl8 Omcsotci8 F-Grubtcsl8 Zndemcsl, F- ^yicsl8 I-

Drdjomcsl8 Rdbbdrcsl b tot hnum tnlc pnma pnp Vymfdii.

Vma jmn eo trma ORP? sdu hno tot hnum, mauo to 700B, bnc et

trcma bb maum ma trm vc sdc bnc mma ma trcma eo trma i 4%.

:.4.2. Fma b v nd bnt

Jma :.:,4

Deecmodb :>

m- Jutdmci 0,>4 447-44>Jutyidbltdt 0,>>0- 0,>>< 441-44>Bicrcgcre 4,610- 4,6>0 70- 7:

Fobicrceltndm 4,2: 2?,7


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4?

Foeltnyigcredeof 0,?67- 0,?,2

Ltnyidbltdt 0,?0: 10-1:

m- Nlxdm 0,7 7?Eltndmci 0,1?4- 0,1?2 76, >?

* Jm ema sb h; Rb h ip ema (VIB) b tnb nom trm jm ema

trma sm FB-Diucgciolm 70 G:0.

Hmn nom vo om t (Wom v somn fbn t Vruma ma).

Ey c mnot mma bny L:-Elit.

Ey \W- WOR Notdbno \-4?00.

Bb fma b hnb mn; Vnb hp Pdielr ( bnmn xb 0,0:ee), np

Pltro, Jurlt, jmn bnot, pdtuym, poplt bb ico, ma manoe, jmn mm, ma cma, bb

b e, aoy ib, qul by, aoy tn pN, m bm, d tny tomn, hoe eo eb,

:.:. PNMA PNP VNB MANOE

:.:.4. Pnma pnp muo by v ao aoma

Bnma Rtrlptceybls 4>2.::4 b by zoazda trm ma tnbn manoma, bnc

vc t e :>-200B, sdu 7-40 may bnma pnt trom tt, bnc bb ma aoma vc t

imn jc qum :-60B, mn h 2-7 tnma by truym et im.


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:0

:.:.:. Pnma pnp pnm ico x hnum tnlc ORP

Bnum j aoma ab Rtrlptceybls 4>2.::4b pnm ip, mauym ab.

^b mn b oe nmn tno, eu sb bd hnum ty b bnt, hnum ty hn

somn v mn ao hn mma tc tnmn sb t nd tdm; Bb EV ORP4, ORP:, ORP2,

ORP6, ORP2.::4im j et tnbn. 200B, tom nmn qudm st

sdu hno muo by b 1, 46, :4 may.

Nmn fma bnuo v j et jc t qudm st fo hmn nom vo om t;

- Fma bnuo jc t; Vnma (Z), tnma no bcma (ZG), eb bu (ZD), xcm b

(R).

- J et jc t b tn b bb fma sdu; Pnma, mnm (se)8 sm so (wd), b ado

(sp), b tb (nd).

Eu bd hnum ty hn somn (eu bd j et hnum ib) ; B tn b eu

(Z), vma (_), xdmn i by (A), xdmn fd tro (J), te (W), xe (Ay), trma (Q).

Vrma np b bb eu xlm h tn ano bb eu iom mndu.

Eu bd hnum ty b bnt; Tudm st et sdu bd hnum ib (et fo d

Pltro), tnma b bb eu vma mu, vma mu mn ncb fd bde, vma mu mn

xdmn fd tro ncb te, vma mu im xdmn i by. Mu tny bb eu trm h nou i(4), hnma tny h nou i (0).

Rb t nd tdm me mady trcma eo trma b bb eu; Wma, xdmn, ,

teMu b h nou i (4), hnma b h nou i (0).

^b mn sb t elidmcof (eu lm ncb eu mu lm); Muo by x

hnum trm bb EV ORP7, ORP1, qudm st may tn :, may tn 6, mu b h nou i

(4), hnma b h nou i (0).

Hn mma tou tn maum bdrjcm; Muo by jc t trm bb eo trmaEV ORP?b bnd bb maum ma hnb mndu, mn ao may tn 4:, 47. Mu

jc t pnt trom emn nm ncb jma hno muo by trm EV ORP?bnd aiubcsl

(bnma fma) tn h nou i (+), mu pnt trom yu nm ncb jma hno muo by trm


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:4

EV ORP?hnma bnc maum ma (bnma e) tn h nou i (-), mu pnt trom

emn nm bnma e v he nm bnma fma tn h nou i ().

:.:.2. mn ao nct tmn hnma somn jma pnma pnp hnubn tm

Mauym tb; Eu tn b t trm ip tnbn fomn fma b bnd WRW hoe

mn. HR t eu tn hnubn tm vc EV tnbn s b bn s pnt trom bd WRW

hoe mn tc tnmn vma v hnum, v me.

Vom nmn;

Ht qu b tmn tnlc bma tnb;

:

4 4

( )

4

m m

o oo o

F F FF s

m m

; ma hmn truma jmn vma v hnum.

Fo; ma hmn vma v hnum tn o.s; ibn tnb manoe bnum b nou bnmn.

m; R tn manoe ie scma scma (hnma b nma fm hnb tn m52).

Bnum j EV muo by WRW hoe mn

(EV bdmn tndma ( WH), ff tc mn fbn (vo me ), nm fbn WRW hoe mn)

d eu tn vc d Pltro b bnd WRW hoe mn

(Hncdmn aoy, aoma tnbn, hno tnbn)

Muo by

WH (210B8 4>- :6n )8 vo me ( :>-200B/:6- 1:n)

mn ao ht qu

c ma hmn vma v hnum, v me.


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:.:.6. Pnma pnp xb mn eo trma muo by tnbn np

Vrcma t by v trma , Rtrlptceybls4>2.::4b by zoazda im EV4 m

EV1 trcma d Pltro, vc t e 7 may. Rdu tn nct tmn HR jma pnma

pnp hno tnbn. Fd vc ht qu bnm rd EV bnc nct tmn HR bdc mnt i EV muo

by tnbn np .

:.:.< . Bnm bnma b nct tmn bdc jma pnma pnp bnm ib mau mnom

Bnum j EV; Bm EV:, tot hnum 4:00B/ 20 pnt, rd d Pltro v

trma.

Pnd nm fbn jc t; iy et vma qul by jc t Rtrlptceybls 4>2.::4

bnc vc ma manoe bnd 40ei mb v trma, ib u, tnu b nm fbn jc t b

pnd icma 40-4( mn b). Rdu pnd icma top m mma 40 -7jma mb v

trma.

Pnm tm nm fbn jc t; nt bnmn xb 0,4 ei nm fbn jc t ma

manoe b pnd icma 40-6m 40-7mn vc d Pltro bnd EV. Fma qul bndma

v trma fm u nm fbn jc t trm j et tnbn. Eo mma tom nmn trm 2

d. Bnc bb d pnm tm jc t vc t e :>0B.

Rdu 7 may, bb hnum ib x hnum pnt trom, bnm bb hnum ib pnt

trom d fma, eb roma r by zoazda im d Pltro b EV, vc t e 7 may rotn nct tmn HR jma pnma pnp hno tnbn. Fd vc ht qu ma hmn vma

v hnum id bnm v ao io 2 fma bnma b nct tmn HR tt mnt bnc manom bu

top tnlc.

:.:.7. Pnma pnp t jom jma mn sma \W

Pnd nm fbn jc t; Iy et vma qul by jc t Rtrlptceybls 4>2.::4

bnc vc ma manoe bnd 40ei mb v trma, ib u, b nm fbn jc t b

pnd icma 40

-4

( mn b ). Rdu fma 4ei pnd icma top m mma 40

-7

romn vc d Pltro ie eu bnma.

t jom jma mn sma \W; ? ei bm io bd mma 40-4 rd d Pltro

v trma. Bnou mn sma \W (5:


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:2

70be - < pnt). Iy rd, vc bn to : ao, fma poplt v trma pnd icma m

mma 40-2.::4muo by trm tnbn

manoma (EV:) b 7 may, by v trma sdma jmn mm 0,4cB,tb qudy

460 vma/pnt trcma 6>n.

Rdu 6>n trm ey ib, aoma bp 4 b by v trma sdma bb jmn mm

0,4cB, tb qudy 460 vma/pnt trcma 4:0n.

Rdu 4:0n ib ncb io te fbn im elm ro tn nct tmn HR jma pnma

pnp aoma tnbn.


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:.:.>. Pnma pnp bnot hnma somn t fbn ib jma fuma eo nu b

Fbn im elm sdu hno ib ico j somn hno b bnmn v pN tnbn np jma

fuma fbn MdCN 4M v NBi 4M ro bnot vo tma fuma eo bnot.

Fbn ib v fuma eo nu b bnc vc jmn am vo t i W fuma eo ; Wfbn ib 5

4;


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:2.::4

^ hnum Rtrlptceybls 4>2.::4b muo by trm EV ORP, sdu :4 mayle qudm st fo hmn nom vo om t nmn tno bnuo jc t v nmn fma j et

jc t ( pnma o 40.000 - 60.000 im). Fd vc ht qu muo by trm EV

ORP, bnma to xb mn b b trma bd Rtrlptceybls 4>2.::4mn sdu;

- Eu bd hnum ty hn somn; Wma trma (_Q).

- Nmn fma bnuo jc t; Vnma no bcma (ZG).

- J et bnuo jc t; Rm so (wd).

o bnou vo bb b oe bd bnma x hnum Rtrlptceybls 4>2.::4vo

bb ico x hnum trcma hnd pnm ico ORP, ht qu b aoo tnou trcma jma 2.4

nmn P6 v nmn P< pnm pn ib.

Jma 2. 4. b oe tnlc ORP bd Rtrlptceybls 4>2.::4vRtrlptceybls

gidvlsblms

b oe pnm ico Rtrlptceybls 4>2.::4 Rtrlptceybls gidvlsblmsEu hnum ty hn somn Wma trma (_Q) _QEu hnum ty b bnt 4 4

Rb t nd tdm 4 4Rb t elidmcof 0 0

Bnuo jc t Vnma no bcma (ZG) ZGDJ et jc t Rm so (wd) ReI - Drdjomcsl + +

F - ^yicsl + Omcsotci -

F - Edmmotci F - Grubtcsl + Zndemcsl

Rdbbndrcsl Zdggomcsl

Mnm xt; Fd vc ht qu b tn tny Rtrlptceybls 4>2.::4b bb b oe

am aoma mnt vo Rtrlptceybls gidvlsblms(40/46 b oe). Vuy mnom bm pno

tnb nom tne bb manom bu hnma mn tm bd bnma Rtrlptceybls 4>2.::4.


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2.:. HN MMA ROMN VMA NP HNMA ROMN BDRVZLPVCE_BLR

4>2.::4VZM EV PNM IP- EV :

Muo by bnma Rtrlptceybls 4>2.::4trm EV: tnbn d, tn nct tmn

HR jma pnma pnp hno tnbn. Ht qu b aoo tnou trcma jma 2.:.

Jma 2. :. Ht qu nct tmn hnma somn bd Rtrlptceybls 4>2.::4trm EV:.

Htqu

J.blrlus

J.sujtoios

J.pueoios

R.durlus

L.bcio

P.dlruaomcsl

R.gilxmlro

P.eordjoios

F(ee)

:4,60 :4,>: :2,>: :4,2> - 4>,27 :4,76 :0,>0

Fd vc ht qu tn nct tmn, to bnm bnma Rtrlptceybls4>2.::4b pn

tb fma rma trm b Arde (+) v Arde (-).

2.2. HV T\ BNM EO VZMA M\O B_

Eb bn bd vob bnm EV muo by mn ao hn mma somn tma np HR

emn mnt bd bnma Rtrlptcybls 4>2.::4v EV tnbn np bnc qu trmn im elm,

ma tno id bnm : WRW hoe mn bnc bb manom bu top tnlc.

^ hnum Rtrlptceybls 4>2.::4b muo by vc 1 EV trmn jy pnm

trb, sdu 7 may tn tom nmn tn nct tmn HR jma pnma pnp hno tnbn vo

bb WRW hoe mn. Ht qu b trmn jy trcma jma 2.2.

Jma 2. 2. Ht qu id bnm EV muo by

WRW hoemn

Htqu

EV4 EV: EV2 EV6 EV< EV7 EV1

J.blrlus(ee) :4,00 :4,67 41,07 0,00 41,60 4:,20 47,70

s 0, :2,00 :0,70 0,00 :0,2> :0,

s 0,10 0,62 0, 4 0,7< 0,00 4,04 0,4< 0,47


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:?

L. bcio(ee) 0,00 0,00 0,00 0,00 0,00 0,00 0,00

s 0,00 0,00 0,00 0,00 0,00 0,00 0,00

P.

dlruaomcsl

(ee) :4,00 ::,6> 42,77 0,00 46,1> 41,4: ::,17

s 0,:4 0,>7 0,:2 0,00 0,,4 0,64 0,::

R. typno(ee) :0,76 :2,00 47,00 0,00 47,>: 4?,>> 4>,>7

s 0,6: 0, 0,00 4?,4> 4>,:s 0,0> 0,61 0,4: 0,00 0,2? 0,>2 0,47

Mnm xt; Bnma Rrlptceybls 4>2.::4pnt trom v b nct tmn HR bdc trm bb

EV4, EV:, EV1v b nct tmn bdc o voJ. sujtoios v P. eordjoios8 bnm : WH

my ie WH hoe mn bnc manom bu top tnlc.

2.6. HV T\ BO VC AOMA

2.6.4. Ht qu bnm ib mau mnom

Vom nmn sma ib mau mnom tnlc :.:.

Nghiên Cứu Kháng Sinh Được Sinh Tổng Hợp Từ Streptomyces 183.221

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Transcript of Nghiên Cứu Kháng Sinh Được Sinh Tổng Hợp Từ Streptomyces 183.221