NeNew w SSampling/ampling/SSensor ensor IInitiative (nitiative (NeSSINeSSI™)™)

Analytical Applications Analytical Applications and Web Toolsand Web Tools

Dave Veltkamp, CPACDave Veltkamp, CPAC

Overview of TalkOverview of TalkDiscuss how NeSSI™ can facilitate Discuss how NeSSI™ can facilitate laboratory applications and how laboratory applications and how current development fits lab needscurrent development fits lab needs

Examples of NeSSI™ as a platform for Examples of NeSSI™ as a platform for micro analytical devices and analyzersmicro analytical devices and analyzers

Mention some web-based resources at Mention some web-based resources at CPACCPAC

What does NeSSI™ ProvideWhat does NeSSI™ ProvideSimple “Lego-like” assemblySimple “Lego-like” assembly

Easy to re-configureEasy to re-configureNo special tools or skills requiredNo special tools or skills required

Standardized flow componentsStandardized flow components““Mix-and-match” compatibility between vendorsMix-and-match” compatibility between vendorsGrowing list of componentsGrowing list of components

Standardized electrical and communication Standardized electrical and communication (Gen II)(Gen II)

““Plug-and-play” integration of multiple devicesPlug-and-play” integration of multiple devicesSimplified interface for programmatic I/O and controlSimplified interface for programmatic I/O and control

Advanced analytics (Gen III)Advanced analytics (Gen III)Micro-analyzersMicro-analyzersIntegrated analysis or “smart” systemsIntegrated analysis or “smart” systems

NeSSI™: Enabler for NeSSI™: Enabler for MicroAnalytical MicroAnalytical

(the “rail” concept)(the “rail” concept)

Standard Mechanical Interface “Rail”

Standard Electrical (Digital) Interface “Rail”

Anyone’s Sensor Anyone’s Actuator

SAM*

Standard “hockey-puck

PC”P

V

*Sensor/Actuator Manager

Standard“connectivity

”

Where Does NeSSI™ Fit in the Where Does NeSSI™ Fit in the LabLab

Instrument/Sensor InterfacesInstrument/Sensor InterfacesDesign standards make development simplerDesign standards make development simpler

Reduced toolset to be masteredReduced toolset to be mastered Reduced sample variability to account forReduced sample variability to account for

Calibration/validation built-inCalibration/validation built-in Consistent physical environment for measurementConsistent physical environment for measurement Stream switching and/or mixing allow generation of Stream switching and/or mixing allow generation of

standards to match analytical requirementsstandards to match analytical requirements

Reaction monitoringReaction monitoringMicroreactors and continuous flow reactorsMicroreactors and continuous flow reactorsBatch reactors (with fast loop)Batch reactors (with fast loop)

Sample PreparationSample PreparationGas handling (mixing, generation, delivery)Gas handling (mixing, generation, delivery)Liquid handling (mixing, dilution, conditioning, etc.)Liquid handling (mixing, dilution, conditioning, etc.)

Special Lab NeSSI™ Special Lab NeSSI™ RequirementsRequirements

Flowing streamsFlowing streamsNeed to move liquid and gas streams through Need to move liquid and gas streams through NeSSI™ NeSSI™ Most laboratory analysis involves discrete samples Most laboratory analysis involves discrete samples rather than sample streamsrather than sample streamsAnalytical characterization of flow in NeSSI™ Analytical characterization of flow in NeSSI™ neededneeded

Heating and CoolingHeating and CoolingLaboratory applications need bothLaboratory applications need bothMay require more rapid thermal responseMay require more rapid thermal response

Integration with other lab apparatusIntegration with other lab apparatusSerial communication and legacy I/OSerial communication and legacy I/OIntegration with lab software systemsIntegration with lab software systems

Low costLow costCompeting with other solutions that don’t carry Competing with other solutions that don’t carry some of NeSSI’s more exotic featuressome of NeSSI’s more exotic features

NeSSI™ and NeSSI™ and MicroreactorsMicroreactors

Investigate integration of NeSSI™ with Investigate integration of NeSSI™ with continuous flow microreactorscontinuous flow microreactors

Initially as analyzer interfaceInitially as analyzer interface

Eventually as complete fluidics frameworkEventually as complete fluidics framework

Automation requires Gen IIAutomation requires Gen II

Initial Sensor IntegrationInitial Sensor Integration

Online RamanProbe

Sample FlowCell

ContinuousMicroreactor

Initial Sensor IntegrationInitial Sensor Integration

NeSSI Raman Sampling NeSSI Raman Sampling BlockBlock

• Parker Interflow NeSSI substrate• Sample conditioning to induce

backpressure to reduce bubble formation and the heated substrate allows analysis at reactor conditions

NESSI Microreactor NESSI Microreactor Sampling/Calibration Sampling/Calibration

SystemSystemReactor Feed 1

Reactor Feed 2

Product Stream

Real-timeCalibration

prod

RamanProbe

Pump 1

Pump 2

waste

Benefits of NeSSI™ to Benefits of NeSSI™ to Reaction AnalysisReaction Analysis

Conditioning and manipulation Conditioning and manipulation of sample introduced to analyzerof sample introduced to analyzerBetter control of sample physical Better control of sample physical parametersparameters

PhasePhaseTemperatureTemperatureVelocityVelocity

Ability to implement sensors at points Ability to implement sensors at points where measurement parameters are where measurement parameters are optimaloptimalFlexibility for performing calibration online Flexibility for performing calibration online without removing sensorwithout removing sensorFast switching of streams for Fast switching of streams for measurement, calibration or validationmeasurement, calibration or validation

NeSSI™ Fermentation NeSSI™ Fermentation SystemSystem

Switching filter system to handle Switching filter system to handle particulate laden liquid streamsparticulate laden liquid streams

Cells in fermentation brothCells in fermentation broth

Supplied by Bob Sherman, CircorSupplied by Bob Sherman, Circor

Interested in plugging, cleaning, and Interested in plugging, cleaning, and other issues related to bio/pharma other issues related to bio/pharma applicationsapplications

2 Filters

Fermentation Headspace Fermentation Headspace SystemSystem

Designed to allow fermentation Designed to allow fermentation headspace monitoringheadspace monitoring

Fast circulation loop of headspace gasesFast circulation loop of headspace gases

Addition of calibration gasesAddition of calibration gases

Output to process Mass spectrometerOutput to process Mass spectrometer

Fermentor Headspace Loop

Calibration gases

ToMassSpec

MFC

MFC

MFC

MFC

MFC

Example Sensor InterfaceExample Sensor InterfaceSensor is a vapochromatic compoundSensor is a vapochromatic compound

Responds to different compounds by Responds to different compounds by intensity and wavelength shifts in intensity and wavelength shifts in fluorescence signalfluorescence signalOptical detection using simple VIS Optical detection using simple VIS spectrometerspectrometer

LED excitation light sourceLED excitation light source Simple reflection optical fiber light pathSimple reflection optical fiber light path

Use of BallProbe to provide single-sided Use of BallProbe to provide single-sided optical interfaceoptical interface

Vapochrome coated on ball surfaceVapochrome coated on ball surface

NeSSI™ system to control delivery and NeSSI™ system to control delivery and mixing of gas streammixing of gas stream

NeSSI Gas Mixing SystemNeSSI Gas Mixing System Using a customized ballprobe reflectance probe for vapochromic detection

- Ocean optics spectrometer- ballprobe coated with vapochrome

- NeSSI substrate with 2 MFC’s

NeSSI Gas Mixing SystemNeSSI Gas Mixing System

Fluorescence response of vapochrome to dry N2 and cylinder air

200

400

600

800

600 650 700 750 800 850

200

400

600

800

600 650 700 750 800 850

Wavelength (nm)

Inte

nsi

ty (

counts

)

Dry N2

Air

Experiment- 25 ms- 20 accums

Air

Experiment- 25 ms- 20 accums

Vapochromic Response Vapochromic Response MovieMovie

Vapochrome SummaryVapochrome Summary

Use of NeSSI™ system allowed for Use of NeSSI™ system allowed for better control and characterization of better control and characterization of sensor responsesensor response

Less variability and faster response to gas Less variability and faster response to gas composition changescomposition changesAbility to generate gas mixtures Ability to generate gas mixtures in situin situ

Opens up potential for additional Opens up potential for additional studies to quantitatively characterize a studies to quantitatively characterize a library of vapochromatic compounds to library of vapochromatic compounds to different analytesdifferent analytesInvestigate optimal sensor designInvestigate optimal sensor design

NeSSINeSSI™™ Knowledge Network Knowledge Network

Main topic page divided into Main topic page divided into 4 sections4 sections

News and InformationNews and Information

Discussion (NeSSI Q&A)Discussion (NeSSI Q&A)

Component ListComponent List Only Manufacturers group may Only Manufacturers group may

post herepost here

Testing and ExamplesTesting and Examples

Main NeSSI™ web siteMain NeSSI™ web sitehttp://http://www.cpac.washington.edu/NeSSI.htmwww.cpac.washington.edu/NeSSI.htm

NKN can be found atNKN can be found athttp://http://www.cpac.washington.edu/NeSSI/NKN.htmlwww.cpac.washington.edu/NeSSI/NKN.html

Component List Sub-topicsComponent List Sub-topicsPage shown from the “Valve, Manual” Page shown from the “Valve, Manual” topictopic

Listing of topics under manual valve main Listing of topics under manual valve main topictopic

Number of posts will correspond to Number of posts will correspond to different available componentsdifferent available components

Component List PageComponent List PageExample post Example post from the from the component component list pagelist page

URL from URL from VendorVendorSpec. sheet Spec. sheet or linkor linkPicture, Picture, description, or description, or link to more link to more informationinformation

Other Vendors are currently working to Other Vendors are currently working to populate the list with more componentspopulate the list with more components

User Self-RegistrationUser Self-RegistrationAllows users to setup accountsAllows users to setup accounts

Selecting to join Selecting to join “Manufacturers” group will “Manufacturers” group will queue user’s registration until a queue user’s registration until a moderator approves it.moderator approves it.Membership in other groups Membership in other groups automatically approved automatically approved immediately immediately

Profile data will be used for Profile data will be used for NeSSI™ mailing listNeSSI™ mailing list

Check the options available from Check the options available from the “Edit Profile” link once user the “Edit Profile” link once user activates accountactivates account

Once user submits registration Once user submits registration form, they are sent an email form, they are sent an email with instructions for activating with instructions for activating their new accounttheir new account

Your account is not active till Your account is not active till you log in and follow the you log in and follow the instructions in the emailinstructions in the email

NKN ConclusionNKN ConclusionNew software and web site ready to be New software and web site ready to be usedused

Need to work on additional instruction sheetsNeed to work on additional instruction sheetsThanks to Michelle Cohn, UOP for helping CPAC Thanks to Michelle Cohn, UOP for helping CPAC buy a new server to host NeSSI™ and NKN web buy a new server to host NeSSI™ and NKN web sitessites

NeSSI Component List is becoming NeSSI Component List is becoming populatedpopulated

Major manufacturers already signed up and Major manufacturers already signed up and starting to supply contentstarting to supply contentNeed to get smaller suppliers to do the sameNeed to get smaller suppliers to do the same

Need to get user community to register Need to get user community to register and start using itand start using it

Expect Email announcements soon (1-2 weeks)Expect Email announcements soon (1-2 weeks)

NeSSI™ Sensor SurveyNeSSI™ Sensor Survey

The purpose of this survey is to gather The purpose of this survey is to gather information on the sensors and information on the sensors and actuators needed for NeSSI Generation actuators needed for NeSSI Generation II Sample Systems, Analytical Systems, II Sample Systems, Analytical Systems, and Cluster Applications.  and Cluster Applications. 

This data will be shared with sensor and This data will be shared with sensor and instrument vendors to help them prioritize instrument vendors to help them prioritize development of the devices needed across development of the devices needed across industries and applications.  industries and applications.  The more information you can provide, the The more information you can provide, the faster better products and performance faster better products and performance can be delivered can be delivered

NeSSI™ Sensor Survey NeSSI™ Sensor Survey (cont.)(cont.)

On-line survey form on the CPAC On-line survey form on the CPAC NeSSI™ web siteNeSSI™ web site

http://www.cpac.washington.edu/NeSSI/Chttp://www.cpac.washington.edu/NeSSI/CPAC_NeSSI_Sensor_Survey.htmPAC_NeSSI_Sensor_Survey.htm

Questions about condition sensorsQuestions about condition sensorsAre you interestedAre you interestedHow would you rank their importanceHow would you rank their importanceOperating rangesOperating ranges

Questions about analytical sensorsQuestions about analytical sensorsAre you interestedAre you interestedHow would you rank their importanceHow would you rank their importanceOperating rangesOperating ranges

Questions about actuatorsQuestions about actuatorsQuestions about “Clusters”Questions about “Clusters”Optional contact informationOptional contact information

NeSSI™ Sensor Survey NeSSI™ Sensor Survey ResultsResults

Condition SensorsCondition Sensors

Analytical SensorsAnalytical Sensors

Count Mean Rank Median RankPressure 14 3.9 4Temp 14 3.9 4Flow 15 4.0 5Humidity 13 3.2 3Cond. 9 2.6 3Other 6 3.0 3Combined 13 3.5 3

O2 NOx pH H2 CO CO2 Raman NIRCount 13 7 10 13 10 8 7 12

Mean Rank 3.8 3.4 3.0 3.5 3.3 3.1 3.0 3.2Med Rank 4 4 3 3 3 3 3 3

MIR UV/Vis NMR MS GC LC ViscosityCount 11 10 3 7 12 2 7

Mean Rank 3.3 2.9 2.0 3.1 4.0 2.5 3.0Med Rank 3 3 1 3 4 2.5 3

NeSSI™ Sensor Survey NeSSI™ Sensor Survey ResultsResults

ActuatorsActuators

ClustersClusters

11 of the 15 respondents indicated they 11 of the 15 respondents indicated they would share information with Vendorswould share information with Vendors

Count Mean Rank Median RankOn/Off Valve 15 3.7 4 Ball 11 Diaphragm 11Modulator Valve 11 3.7 4Solenoid Valve 12 3.3 3MFC 13 3.8 4VFC 11 3.6 3

CountCEMS 9Clean Water 8Waste Water 6HRVOC 5

NeSSI™ Sensor Survey NeSSI™ Sensor Survey (cont.)(cont.)

Plan is to continue to solicit responses from Plan is to continue to solicit responses from usersusers

Additions to survey questions or content welcomeAdditions to survey questions or content welcome

May expand survey activities to help provide May expand survey activities to help provide market directions to suppliersmarket directions to suppliers

Other aspects of NeSSI™ developmentOther aspects of NeSSI™ development Applet Software and other issues relate to SAMApplet Software and other issues relate to SAM New opportunities or innovationsNew opportunities or innovations

Need to reach beyond the normal NeSSI™ Need to reach beyond the normal NeSSI™ enthusiastsenthusiasts

Combined with the discussion board, surveys Combined with the discussion board, surveys provide a valuable resource for both end-provide a valuable resource for both end-users and manufacturers of NeSSIusers and manufacturers of NeSSI

Summary & ConclusionSummary & ConclusionNeSSI™ Gen II ready for developmentNeSSI™ Gen II ready for development

Working spec/prototype design completeWorking spec/prototype design complete

Prototypes demonstrated in real Prototypes demonstrated in real applicationsapplications

Near resolution of the NeSSI™ bus issueNear resolution of the NeSSI™ bus issue

For some laboratory applicationsFor some laboratory applicationsGen II and Gen III will immediately work Gen II and Gen III will immediately work and provide benefitsand provide benefits

The future holds a lot of promise!!The future holds a lot of promise!!