NEUROSCIENCE MARKER PANEL - Atlas Antibodies
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NEUROSCIENCE MARKER PANEL
Transcript of NEUROSCIENCE MARKER PANEL - Atlas Antibodies
NEUROSCIENCE MARKER PANEL
NEW PRODUCTS
Microglia markers
Astroc
ytes markers
Oligo
dendrocytes markers
NEUROINFLAMMATION
NEW MONOCLONAL ANTIBODIESNEW MONOCLONAL ANTIBODIESRead about our newly released antibodies markers on pages 6-7 3737
INDEXpage 3 ANTIBODIES FOR NEUROSCIENCE page 4 WHY ATLAS ANTIBODIES page 6 NEUROINFLAMMATION - SELECTED MARKERS page 8 SYNAPTIC SIGNALING - SELECTED MARKERS page 10 NEURAL LINEAGE- SELECTED MARKERS page 12 NEURODEGENERATIVE DISEASES - SELECTED MARKERS page 14 DEVELOPMENT - SELECTED MARKERS page 16 ENHANCED VALIDATION
Antibodies are valuable tools in the ongoing scientific effort to gain more knowledge about the human brain to understand the fundamental and emergent properties of neurons and neural circuits and to identify diseases such as neurological brain disorders Therefore there is a necessary need for highly-characterized and specific antibodies for neuroscience research
Our neuroscience catalog features a wide range of primary monoclonal and polyclonal antibodies for use in research designed to recognize the diverse neurochemical cell types in rodent and human nervous systems validated in cell lines rodents and human tissues
Our antibodies are validated for use in IHC WB and ICC-IF
ANTIBODIES FOR NEUROSCIENCE
ImmunofluorescenceWestern blot
Im
munohistochemistry3
VERY RELIABLE ANTIBODIESAtlas Antibodies is the original manufacturer of over 21000 primary antibodies targeting the majority of human proteins Our Triple A PolyclonalsTM and PrecisA MonoclonalsTM antibodies are available worldwide Building on our heritage with the Human Protein Atlas project we provide highly validated reagents that enable leading research in biology diagnostics and medicine for the purpose of understanding and improving human health
CREATED BY THE HUMAN PROTEIN ATLASTriple A Polyclonals are developed within the prestigious Human Protein Atlas project Our antibodies are validated in all major human tissues and organs and 20 cancer tissues Each antibody is supported by over 500 staining images
VALIDATED BY ENHANCED VALIDATIONWe take great care to validate our antibodies in IHC WB and ICC-IF As an additional layer of security we perform Enhanced Validation By using 5 different enhanced validation methods we validate our antibodies for each combination of protein sample and application
EVIDENCED BY SCIENCEOur antibodies are used all over the world and referenced in 1000s of scientific peer-reviewed papers
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WHY ATLAS ANTIBODIES
The Human Protein Atlas
Atlas Antibodies
Learn more
NEUROINFLAMMATIONNeuroinflammation broadly defines the collective reactive immune response in the brain and spinal cord in response to injury and disease Inflammation in the central nervous system (CNS) is commonly associated with various degrees of tissue damage such as loss of myelin and neurons
The neuroinflammatory process is complex and involves disruption of the blood-brain barrier peripheral leukocyte infiltration edema and gliosis The inflammatory response is characterized by a host of cellular and molecular aberrations within the brain
Neuroinflammation arises locally within the CNS through phenotypic changes of different non-neuronal cell types in the brain such as microglia oligodendrocytes and astrocytes causing the release of different cytokines and chemokines and finally recruitment and infiltration of peripheral blood cells mainly T-and B-cells into the brain parenchyma
Neuroinflammatory processes are the key causative factors behind the brain and spinal cord injury This is true not only for acute brain trauma and hypoxic-ischemic brain damage following stroke but also for chronic infection and neurodegenerative diseases such as Alzheimerrsquos disease (AD) amyotrophic lateral sclerosis (ALS) Lewy body dementia (LBD) and multiple sclerosis (MS) In addition local peritumoral inflammation plays a role in the clinical progression and malignancy of glioblastomas the most aggressive primary brain tumors
Ant
i-GFA
P Anti-MBP Anti-P2RY12
Anti-
HLA-DRA antibody
Anti-
HLA-DRA Anti-GFAP
Anti-HLA-DRA (AMAb91674) shows microglial cells in red Anti-GFAP (AMAb91033) shows
astrocytes in green in Alzheimerrsquos disease cerebral cortex
Anti-P2RY12 (HPA014518) shows microglial cells in green Anti-GFAP(AMAb91033) shows
astrocytes in blue Anti-MBP (AMAb91062) shows myelinated processes in red in
Alzheimerrsquos disease cerebral cortex
Anti-HLA-DRA monoclonal antibody (AMAb91674) shows strong positivity in
microglial cells in Alzheimerrsquos disease cerebral cortex
6
New PrecisA MonoclonalsTM for Neuroinflammation
Products with enhanced validation for indicated application (see page 16)
7
Product Name
Product Number Clonality Validated
ApplicationsAnti-AGER AMAb91634 Monoclonal IgG2a IHC
Anti-AGER AMAb91635 Monoclonal IgG1 IHC
Anti-AIF1 AMAb91671 Monoclonal IgG2b IHC
Anti-AIF1 AMAb91672 Monoclonal IgG1 IHC
Anti-AMIGO3 AMAb91677 Monoclonal IgG1 WB
Anti-ANO2 AMAb91641 Monoclonal IgG1 IHC
Anti-ANO2 AMAb91642 Monoclonal IgG1 IHC
Anti-CD163 AMAb91646 Monoclonal IgG1 IHCWB
Anti-CD163 AMAb91648 Monoclonal IgG1 IHC
Anti-CRYAB AMAb91661 Monoclonal IgG1 IHC WB ICC-IF
Anti-CRYAB AMAb91662 Monoclonal IgG2b IHC WB
Anti-CXCL13 AMAb91629 Monoclonal IgG1 IHC
Anti-DYSF AMAb91667 Monoclonal IgG1 IHC WB
Anti-GAP43 AMAb91664 Monoclonal IgG2b IHC
Anti-GAP43 AMAb91665 Monoclonal IgG1 IHC
Anti-GAP43 AMAb91666 Monoclonal IgG2a IHC
Anti-GPR17 AMAb91624 Monoclonal IgG1 IHC WB
Anti-GZMB AMAb91650 Monoclonal IgG1 IHC
Anti-HLA-DRA AMAb91673 Monoclonal IgG1 IHC WB
Anti-HLA-DRA AMAb91674 Monoclonal IgG2a IHC WB
Anti-HLA-DRA AMAb91675 Monoclonal IgG2b IHC WB
Anti-IL17A AMAb91615 Monoclonal IgG1 IHC
Anti-IL17RA AMAb91617 Monoclonal IgG2b WB
Anti-IL17RA AMAb91619 Monoclonal IgG1 WB
Anti-IL7 AMAb91684 Monoclonal IgG1 ICC-IF
Anti-ITGA4 AMAb91699 Monoclonal IgG2b WB
Anti-MS4A1 AMAb91636 Monoclonal IgG2a IHC WB
Anti-OPALIN AMAb91685 Monoclonal IgG1 IHC
Anti-OPALIN AMAb91686 Monoclonal IgG2a IHC
Anti-PLP1 AMAb91639 Monoclonal IgG1 IHC
Anti-RGMA AMAb91702 Monoclonal IgG2b WB
Anti-S100A8 AMAb91689 Monoclonal IgG1 IHC
Anti-S100A9 AMAb91690 Monoclonal IgG2a IHC
Anti-SERPINA3 AMAb91655 Monoclonal IgG1 IHC
Anti-SPP1 AMAb91653 Monoclonal IgG1 IHC
Anti-TLR2 AMAb91631 Monoclonal IgG1 WB
Anti-TRPM4 AMAb91693 Monoclonal IgG1 IHC WB ICC-IF
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Anti-AGER HPA064436 Polyclonal WB ICC-IF 41 41
Anti-AIF1 HPA049234 Polyclonal IHC 84 84
Anti-AIF1 HPA062949 Polyclonal ICC-IF 86 83
Anti-ANO2 HPA057499 Polyclonal IHC 84 85
Anti-CD163 HPA046404 Polyclonal IHC ICC-IF 51 30
Anti-CD163 HPA051974 Polyclonal IHC 75 83
Anti-CRYAB HPA057100 Polyclonal IHC 98 98
Anti-DYSF HPA017071 Polyclonal IHC ICC-IF 96 96
Anti-DYSF HPA021945 Polyclonal IHC 88 88
Anti-GAP43 HPA013392 Polyclonal IHC ICC-IF 88 86
Anti-GAP43 HPA015600 Polyclonal IHC ICC-IF 71 70
Anti-GPR17 HPA029766 Polyclonal IHC 92 92
Anti-GZMB HPA003418 Polyclonal IHC WB 63 63
Anti-HLA-DRA HPA050162 Polyclonal IHC 54 69
Anti-HLA-DRA HPA053176 Polyclonal IHC 41 83
Anti-IL7 HPA019590 Polyclonal IHC 46 46
Anti-ITGA4 HPA074961 Polyclonal ICC-IF 90 89
Anti-MS4A1 HPA014341 Polyclonal IHC WB ICC-IF 73 77
Anti-MS4A1 HPA014391 Polyclonal IHC WB 61 64
Anti-OPALIN HPA014372 Polyclonal IHC 72 75
Anti-PLP1 HPA004128 Polyclonal IHC 100 100
Anti-RGMA HPA039880 Polyclonal ICC-IF 96 96
Anti-RGMA HPA044222 Polyclonal ICC-IF 87 87
Anti-S100A8 HPA024372 Polyclonal IHC WB ICC-IF 56 60
Anti-SERPINA3 HPA000893 Polyclonal IHC WB 61 61
Anti-SERPINA3 HPA002560 Polyclonal IHC WB 60 59
Anti-SPP1 HPA027541 Polyclonal IHC WB ICC-IF 56 53
Anti-TRPM4 HPA041169 Polyclonal IHC ICC-IF 93 91
NEW PRODUCTS
Selected Triple A PolyclonalsTM for Neuroinflammation
Products with enhanced validation for indicated application (see page 16)
Synaptic signaling is cell-cell communication that occurs at the synaptic level Cells communicate with each other via direct contact (juxtacrine signaling) over short distances (paracrine signaling) or vast distances and scales (endocrine signaling) Other cells form gap junctions that connect their cytoplasm to the cytoplasm of adjacent cells
When the sending neuron fires an electrical impulse travels along the axon As soon as the impulse reaches the synapse it triggers the release of neurotransmitters
The predominant excitatory neurotransmitter in the brain is glutamate (GLU) The inhibitory neurotransmitters GABA (in the brain) and glycine (in the brainstem and spinal cord) are common amino acids that participate in multiple metabolic cycles
Calcium (Ca2+) signaling in neurons is critical in cell signalingIt defines the ability of cells to release neurotransmitters thus regulating the intracellular signaling pathways Calbindin 2 (also known as calretinin) is one of the Ca2+-binding proteins involved in Ca2+ signaling that facilitates cytosolic Ca2+ diffusion without interfering with Ca2+ signaling
SYNAPTIC SIGNALING
A
nti-CALB2 antibody
An
ti-mGluR1 antibody
A
nti-VGAT antibody
Anti-VGAT monoclonal antibody (AMAb91043) shows strong immunoreactivity in GABAergic
fibers in the rat globus pallidus in green
Anti-CALB2 polyclonal antibody (HPA007305) shows the labeling in green of mouse
hippocampus Note the strong labeling in the dentate gyrus
Anti-MGLUR1 polyclonal antibody (HPA015701) strongly labels cortical perikarya in human
cerebral cortex tissue in brown
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Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Synaptic Signaling
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Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Anti-ATF2 HPA022134 Polyclonal IHC WB ICC-IF 99 99
Anti-ATF3 AMAb90909 Monoclonal IHC ICC-IF 92 92
Anti-ATF3 HPA001562 Polyclonal IHC ICC-IF 92 92
Anti-ATP1B1 HPA012911 Polyclonal IHC WB 93 93
Anti-ATP1B2 HPA010698 Polyclonal IHC 96 88
Anti-CAMK2B HPA026307 Polyclonal IHC WB 96 96
Anti-CALB1 HPA023099 Polyclonal IHC WB ICC-IF 98 99
Anti-CALB2 HPA007306 Polyclonal IHC WB ICC-IF 100 100
Anti-CALB2 HPA007305 Polyclonal IHC WB ICC-IF 100 100
Anti-CAMK2D HPA026281 Polyclonal IHC 100 97
Anti-C-FOS HPA018531 Polyclonal IHC ICC-IF 94 94
Anti-CHAT AMAb91130 Monoclonal IHC ICC-IF 96 96
Anti-CHAT HPA048547 Polyclonal IHC 96 96
Anti-CHRM1 (M1 mAChR) HPA014101 Polyclonal IHC 98 97
Anti-CHRM2 (M2 AChR) HPA029795 Polyclonal IHC 88 86
Anti-CLIC4 HPA008019 Polyclonal IHC WB 98 97
Anti-CREB1 HPA019150 Polyclonal IHC WB ICC-IF 100 100
Anti-DDC AMAb91089 Monoclonal IHCWB 90 88
Anti-DDC HPA017742 Polyclonal IHCWBICC-IF 90 88
Anti-EAAC1 HPA020086 Polyclonal IHC WB 77 79
Anti-EAAT2 HPA009172 Polyclonal IHC 87 89
Anti-GABRA3 HPA000839 Polyclonal IHCWB 91 93
Anti-GABRB1 HPA051297 Polyclonal IHC 97 100
Anti-GABRG1 HPA035622 Polyclonal IHC 96 94
Anti-GAD1 (GAD67) AMAb91076 Monoclonal IHCWB 94 94
Anti-GAD1 HPA058412 Polyclonal IHCWB 94 94
Anti-GAD2 AMAb91048 Monoclonal IHCWB 84 88
Anti-GAD2 HPA044637 Polyclonal IHC 84 88
Anti-GAT1 HPA013341 Polyclonal IHC WB 98 98
Anti-GAT3 HPA037981 Polyclonal IHCWB 85 87
Anti-GLUR2 (AMPA2) HPA008441 Polyclonal IHC 100 100
Anti-HTR2A HPA014011 Polyclonal IHC 95 97
Anti-KCC4 HPA041652 Polyclonal IHCWB 84 82
Anti-KCNJ5 (KIR34) HPA017353 Polyclonal IHCWB 89 89
Anti-KIF11 HPA010568 Polyclonal IHCWB 88 83
Anti-KIF17 HPA032085 Polyclonal IHCICC-IF 85 82
Anti-KIF18A HPA039312 Polyclonal IHCWBICC-IF 80 82
Anti-KIF1C HPA024602 Polyclonal IHCWB 81 83
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Anti-KIF26B HPA028562 Polyclonal IHCICC-IF 88 80
Anti-KIF4A (KIF4A amp B) HPA034745 Polyclonal IHCWBICC-IF 64 63
Anti-KIF5A HPA004469 Polyclonal IHCWB 91 88
Anti-KIF5C HPA035210 Polyclonal IHCWB 100100
Anti-KIF7 HPA043145 Polyclonal IHC 69 69
Anti-KIFAP3 HPA023742 Polyclonal IHC 100 100
Anti-MAPK1 (ERK) HPA030069 Polyclonal IHCWB 100 100
Anti-MAPK3 (ERK1) HPA005700 Polyclonal IHCWB 98 98
Anti-MGLUR1 HPA015701 Polyclonal IHC 80 80
Anti-MGLUR8 HPA051481 Polyclonal IHC 95 90
Anti-NCS1 HPA019713 Polyclonal IHCWBICC-IF 100 100
Anti-PRKCA HPA006563 Polyclonal IHCWBICC-IF 99 99
Anti-PRKCH HPA053709 Polyclonal IHCICC-IF 97 64
Anti-PNMT HPA051005 Polyclonal IHC 89 92
Anti-PRKACB (PKACB) HPA029754 Polyclonal IHC 73 76
Anti-PRKCZ HPA021851 Polyclonal IHCWB ICC-IF 94 94
Anti-RAB3A HPA003160 Polyclonal IHC 99 99
Anti-RAP1GAP HPA001922 Polyclonal IHC 92 91
Anti-SLC17A7 (VGLUT1) AMAb91041 Monoclonal IHCWB 94 94
Anti-SLC22A2 HPA008567 Polyclonal IHC 84 77
Anti-SLC32A1 (VGAT) HPA058859 Polyclonal IHC 95 93
Anti-SLC6A2 (NET) AMAb91116 Monoclonal IHC -
Anti-SLC6A3 (DAT) AMAb91125 Monoclonal IHC 85 85
Anti-SNAP25 HPA001830 Polyclonal IHCWB 100 100
Anti-SNAP29 HPA031823 Polyclonal IHC 89 92
Anti-SST (SOM) HPA019472 Polyclonal IHCWB 98 98
Anti-STXBP1 HPA008209 Polyclonal IHCWBICC-IF 100 100
Anti-STXBP6 HPA003552 Polyclonal IHCWB 99 99
Anti-SYNGR1 HPA029673 Polyclonal IHC ICC-IF 90 88
Anti-SYNPR HPA061671 Polyclonal IHC 97 97
Anti-SYP HPA002858 Polyclonal IHCWB 83 83
Anti-SYT1 HPA008394 Polyclonal IHCWB 100 100
Anti-SYT12 HPA011006 Polyclonal IHC 96 98
Anti-SYT13 HPA046224 Polyclonal IHC 96 93
Anti-SYT16 HPA004199 Polyclonal IHCWB 95 95
Anti-TGFA HPA042297 Polyclonal IHC 93 93
Anti-TH AMAb91112 Monoclonal IHC 88 88
Anti-TH HPA061003 Polyclonal IHC WB 88 88
Anti-TPH2 (NTPH) AMAb91108 Monoclonal IHC 100 100
Anti-VAMP4 HPA050418 Polyclonal IHCICC-IF 100 100
Anti-VAMP7 HPA036733 Polyclonal IHCICC-IF 98 93
Products with enhanced validation for indicated application (see page 16)
NEURAL LINEAGE
A
nti-GFAP antibody
Anti-C
NP antibody
Anti-N
EFM antibodyDuring central nervous system development neural stem cells (NSCs) proliferate and divide to generate a progeny that differentiate into neurons astrocytes oligodendrocytes and different glial cells
NSCs self-renewal and differentiation are regulated by a precise temporal sequence of growth factors intracellular signaling and transcription factor expression NSCs and their differentiated progeny can be identified by the expression of a unique combination of cell surface markers and transcription factors
The glial fibrillary acidic protein (GFAP) is an intermediate filament (IF) protein and a well-known marker for astrocytes
Oligodendrocytes can convert extracellular 2rsquo3rsquo-cAMP to adenosine As CNPase is the predominant enzyme involved in this conversion the cyclic nucleotide phosphodiesterase (CNP) is widely used as an oligodendrocyte marker
Neurons express the neurofilament medium (NFEM) polypeptide other intermediate filament proteins including nestin synemin syncoilin and vimentin
Anti-GFAP polyclonal antibody (HPA056030) shows strong immunoreactivity in the
human cerebellum in brown
Anti-NEFM polyclonal antibody (HPA022845) shows positivity in a subset of neuronal cells in
human cerebral cortex in brown
Anti-CNP polyclonal antibody (HPA023266) in the rat cerebral cortex is shown in magenta
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Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Neural Lineage
Products with enhanced validation for indicated application (see page 16)
11
TargetCells
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Astrocytes Anti-GFAP AMAb91033 Monoclonal IHC WB 98 100
Astrocytes Anti-GFAP HPA056030 Polyclonal IHCWB ICC-IF 98 100
Astrocytes Anti-GLUL AMAb91101 Monoclonal IHC WB 95 53
Astrocytes Anti-GLUL AMAb91102 Monoclonal IHC WB 95 92
Astrocytes Anti-GLUL AMAb91103 Monoclonal IHC WB 95 92
Astrocytes Anti-GLUL HPA007316 Polyclonal IHCWB 95 53
Astrocytes Anti-S100B AMAb91038 Monoclonal IHC WB 99 98
Astroglia Anti-EZR AMAb90976 Monoclonal IHCWBICC-IF 93 93
Astroglia Anti-EZR HPA021616 Polyclonal IHCWBICC-IF 93 93
Adhesion molecule Anti-ICAM5 HPA009083 Polyclonal IHC 85 86
Adhesion molecule Anti-MCAM HPA008848 Polyclonal IHC WB 75 73
Adhesion molecule Anti-NCAM2 HPA030900 Polyclonal IHC 89 91
Adhesion molecule Anti-NLGN1 HPA006680 Polyclonal IHCWB 98 98
Calcium binding protein Anti-CALB1 (CB) HPA023099 Polyclonal IHCWBICC-IF 98 99
Calcium binding protein Anti-CALB2 (CR) HPA007305 Polyclonal IHCWBICC-IF 100 100
Calcium binding proteins Anti-NECAB1 AMAb90801 Monoclonal IHCWB 98 98
Calcium binding protein Anti-NECAB1 HPA023629 Polyclonal IHCWB 98 98
Calcium binding protein Anti-NECAB2 AMAb90808 Monoclonal IHC 85 84
Calcium binding protein Anti-NECAB2 HPA013998 Polyclonal IHCICC-IF 98 97
Calcium binding protein Anti-SCGN AMAb90630 Monoclonal IHCWB 96 96
Calcium binding protein Anti-SCGN HPA006641 Polyclonal IHC 96 96
Calcium binding protein Anti-S100B HPA015768 Polyclonal IHCWBICC-IF 99 98
Oligodendrocytes Anti-MOG AMAb91066 Monoclonal IHC WB 91 89
Oligodendrocytes Anti-MOG AMAb91067 Monoclonal IHC WB 91 89
Oligodendrocytes Anti-MOG HPA021873 Polyclonal IHC 91 89
Oligodendrocytes Anti-CNP AMAb91068 Monoclonal IHC WB 76 77
Oligodendrocytes Anti-CNP AMAb91069 Monoclonal IHC WB 76 77
Oligodendrocytes Anti-CNP AMAb91072 Monoclonal IHC WB ICC-IF 76 77
Oligodendrocytes Anti-CNP HPA023280 Polyclonal IHCWBICC-IF 76 77
Progenitors Anti-PBK HPA005753 Polyclonal IHCWBICC-IF 91 94
Progenitors Anti-MKI67 (Ki67) AMAb90870 Monoclonal IHC ICC-IF 68 68
Progenitors Anti-MKI67 (Ki67) HPA000451 Polyclonal IHCICC-IF 66 67
TargetCells
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Cytoskeleton Anti-ACTN1 HPA006035 Polyclonal IHCWB 98 99
Cytoskeleton Anti-ACTN4 HPA001873 Polyclonal IHCWBICC-IF 99 98
Cytoskeleton Anti-INA HPA008057 Polyclonal IHC WB ICC-IF 83 84
Cytoskeleton Anti-MAP1A HPA039064 Polyclonal IHC 60 52
Cytoskeleton Anti-MAP1B HPA022275 Polyclonal IHCWB ICC-IF 85 86
Cytoskeleton Anti-MAP2 HPA008273 Polyclonal IHC WB ICC-IF 96 96
Cytoskeleton Anti-MYO5A HPA001356 Polyclonal IHCICC-IF 99 98
Cytoskeleton Anti-NEFM (NF160) HPA022845 Polyclonal IHCWBICC-IF 98 98
Microglia Anti-AIF HPA049234 Polyclonal IHC 84 84
Microglia Anti-CD68 AMAb90874 Monoclonal IHCWB 76 76
Microglia Anti-CD68 HPA048982 Polyclonal IHC WB 76 76
Microglia Anti-ITGAM (CD11b) AMAb90911 Monoclonal IHC WB 67 68
Microglia Anti-PTPRC AMAb90518 Monoclonal IHCWB 35 37
Microglia Anti-PTPRC HPA000440 Polyclonal IHCWB 35 37
Microglia Anti-TMEM119 AMAb91528 Monoclonal IHCWB ICC-IF 52 46
Microglia Anti-TMEM119 HPA051870 Polyclonal IHC 52 46
Neurons Anti-NEFM (NF160) AMAb91027 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91028 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91029 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91030 Monoclonal IHC WB 98 98
Neurons Anti-NEFH (NF200) AMAb91025 Monoclonal IHC WB 98 98
Neurons Anti-NEFH (NF200) HPA061615 Polyclonal IHC 88 94
Neurons Anti-UCHL1 AMAb91145 Monoclonal IHC WB ICC-IF 97 97
Neurons Anti-UCHL1 HPA005993 Polyclonal IHCWBICC-IF 97 97
Neurons Anti-VIM AMAb90516 Monoclonal IHCWB 99 99
Neurons Anti-VIM HPA001762 Polyclonal IHCWBICC-IF 99 99
Schwann cells Anti-MBP AMAb91062 Monoclonal IHC WB ICC-IF 97 97
Schwann cells Anti-MBP AMAb91063 Monoclonal IHC WB ICC-IF 97 97
Schwann cells Anti-MBP AMAb91064 Monoclonal IHC WB 97 97
Anti-I
TM2B antibodyNeurodegenerative diseases such as Amyotrophic Lateral Sclerosis Parkinsonrsquos Alzheimerrsquos and Huntingtonrsquos diseases occur as a result of the progressive loss of structure and function in neurons leading to the death of neurons
Age remains the primary risk factor for almost all the neurodegenerative disorders thus interfering with age-related molecular mechanisms or pathways might be an avenue to develop new therapeutics
The choline acetyltransferase (ChAT) is an enzyme responsible for catalyzing the synthesis of acetylcholine and is expressed in the majority of cholinergic neurons Hence ChAT immunoreactivity is used to detect cognitive decline in several neurodegenerative disorders
Integral membrane protein 2B (ITM2B) is a protein that in humans is encoded by the ITM2B gene This gene located on chromosome 13 is connected to familial dementia causing amyloid and pre-fibrillar effects similar to those seen in Alzheimerrsquos
The expression of the ubiquitin-carboxyl-terminal hydrolase isozyme L1 (UCHL1) present both in the neuronal cells of the central and peripheral nervous system is down-regulated in Parkinsonrsquos and Alzheimerrsquos disease patients
NEURODEGENERATIVE DISEASES
Anti-C
HAT antibody
Anti-U
CHL1 antibody
Anti-CHAT monoclonal antibody (AMAb91130) shows strong immunoreactivity in the
acetylcholine neurons in the rat diagonal band of Broca in green
Anti-ITM2B polyclonal antibody (HPA029292) targeting Integral membrane protein 2B strongly
labels Golgi apparatus in A-431 cells in green
Anti-UCHL1 monoclonal antibody (AMAb91145) in the mouse trigeminal ganglion show strong
immunoreactrivity in primary sensory neurons in green
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Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for NeurodegenerationProduct Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-ADAR AMAb90535 Monoclonal IHCWB ICC-IF 86 85
Anti-ADAR HPA003890 Polyclonal IHCWBICC-IF 86 85
Anti-AIMP1 HPA018476 Polyclonal IHCWBICC-IF 96 97
Anti-AKT1 AMAb90834 Monoclonal WB ICC-IF 97 97
Anti-AOX1 HPA040199 Polyclonal IHC 84 86
Anti-APBA3 HPA045577 Polyclonal IHC 70 68
Anti-APBB2 HPA023542 Polyclonal IHCWBICC-IF 85 83
Anti-APBB3 HPA005571 Polyclonal IHCICC-IF 84 84
Anti-APOA4 AMAb90769 Monoclonal IHC WB 65 28
Anti-APP HPA001462 Polyclonal IHCWB 95 95
Anti-AQP4 AMAb90537 Monoclonal IHCWB 93 92
Anti-AQP4 HPA014784 Polyclonal IHCWB ICC-IF 93 92
Anti-ATF2 HPA022134 Polyclonal IHCWBICC-IF 99 99
Anti-ATF3 AMAb90909 Monoclonal IHCICC-IF 92 92
Anti-ATF3 HPA001562 Polyclonal IHCICC-IF 92 92
Anti-ATRX AMAb90784 Monoclonal IHC WBICC-IF 96 97
Anti-ATXN1 HPA008335 Polyclonal IHC 81 81
Anti-ATXN2 HPA018295 Polyclonal IHCWBICC-IF 90 91
Anti-C3 HPA020432 Polyclonal IHC 78 23
Anti-CASP3 HPA002643 Polyclonal IHCWBICC-IF 84 88
Anti-CD4 AMAb90754 Monoclonal IHCWB 55 57
Anti-CD40 AMAb90905 Monoclonal IHCWB 58 54
Anti-CHAT AMAb91130 Monoclonal IHCICC-IF 96 96
Anti-CHAT HPA048547 Polyclonal IHC 96 96
Anti-CHGA AMAb90525 Monoclonal IHCWBICC-IF 62 64
Anti-COX1 HPA002834 Polyclonal IHCWBICC-IF 93 90
Anti-COX2PTGS2 HPA001335 Polyclonal IHCICC-IF 88 88
Anti-CTSB HPA018156 Polyclonal IHCICC-IF 79 79
Anti-CTSD HPA003001 Polyclonal IHCWB 86 86
Anti-CXorf27 HPA003356 Polyclonal IHC 47 46
Anti-FBXO7 (PARK15) HPA032114 Polyclonal IHC 78 81
Anti-FUS AMAb90549 Monoclonal IHC WB ICC-IF 91 91Anti-GSK3B HPA028017 Polyclonal IHCWBICC-IF 100 100Anti-HIP1 HPA017964 Polyclonal IHCWB 79 77Anti-HTRA2 (PARK13) HPA027366 Polyclonal IHCWB 57 63Anti-ITGAM (CD11b) AMAb90911 Monoclonal IHCWB 67 68
Anti-ITM2B HPA029292 Polyclonal IHC ICC-IF 95 96Anti-LRP2 HPA005980 Polyclonal IHC 78 36
Products with enhanced validation for indicated application (see page 16)
13
Product Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-NFKB1 HPA027305 Polyclonal IHCWBICC-IF 60 62
Anti-OPTN HPA003360 Polyclonal IHCWBICC-IF 64 68
Anti-PADI4 HPA017007 Polyclonal IHCWB 66 69
Anti-PARK7 HPA004190 Polyclonal IHCWB 89 90
Anti-PFN1 AMAb91181 Monoclonal IHCWB 87 87
Anti-PHGDH AMAb90786 Monoclonal IHCWBICC-IF 99 99
Anti-PHGDH HPA021241 Polyclonal IHCWBICC-IF 99 99
Anti-PRNP HPA042754 Polyclonal IHC 91 91
Anti-PSEN1 HPA030760 Polyclonal IHC 82 81
Anti-RHOT1 AMAb90852 Monoclonal IHCWB 100 100
Anti-RHOT1 HPA010687 Polyclonal IHC 100 100
Anti-S100A8 HPA024372 Polyclonal IHCWBICC-IF 56 60
Anti-SERPINA3 HPA002560 Polyclonal IHCWB 60 59
Anti-SNCB HPA035876 Polyclonal IHCWB 97 97
Anti-SOD1 HPA001401 Polyclonal IHCWBICC-IF 82 81
Anti-SOD2 HPA001814 Polyclonal IHCWB 88 87
Anti-THY1 AMAb90844 Monoclonal IHCWB 64 68
Anti-THY1 HPA003733 Polyclonal IHCICC-IF 64 68
Anti-TNFRSF21 HPA006746 Polyclonal IHCWB 86 85
Anti-TTR AMAb90649 Monoclonal IHC WB 81 83
Anti-UBE2K HPA028869 Polyclonal IHCICC-IF 100 100
Anti-UCHL1 HPA005993 Polyclonal IHCWBICC-IF 97 97
Anti-USP46 AMAb90722 Monoclonal WBICC-IF 100 99
Anti-VPS26A AMAb90967 Monoclonal IHC WBICC-IF 96 100
Anti-WHSC1 AMAb90851 Monoclonal IHCWBICC-IF 91 91
Anti-WHSC1 HPA015801 Polyclonal IHCICC-IF 91 91
Anti-VWF AMAb90928 Monoclonal IHCWB 80 80
Anti-VWF HPA002082 Polyclonal IHC 82 78
The development of the central nervous system includes the development of the brain spinal cord optic and auditory systems
Within the neural tube stem cells generate the two major classes of cells that make the majority of the nervous system neurons and glia Both these classes of cells differentiate into many different types generated with highly specialized functions and shapes
The development of the peripheral and central nervous systems begins early in embryogenesis and can be traced through the use of lineage antibodies specific to each stage of neuronal development
The (SOX) family of transcription factors are well-established regulators of cell fate decisions during development
Vimentin (VIM) is a type III intermediate filament protein expressed in mesenchymal cells It is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both healthy development and metastatic progression
The cell surface glycoprotein Thy1 (CD90) is expressed on many mammalian cell types including neurons and serves as a human mesenchymal stem cell (MSC) marker
DEVELOPMENT
A
nti-VIM antibody
A
nti-SOX4 antibody
Anti-T
HY1 antibody
Anti-VIM polyclonal antibody (HPA001762) immunofluorescent staining of human cell line
ASC TERT1 shows localization to intermediate filaments in green
Anti-SOX4 monoclonal antibody (AMAb913802) immunofluorescent staining in mouse
hippocampus showing immunoreactivity in a subset of neurons in the granule cell layer of
the dentate gyrus in brown
Anti-THY1 polyclonal antibody (HPA003733) staining of human cerebral cortex shows
localization to neuropils in brown
Product Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-ENG AMAb90925 Monoclonal IHC 66 22Anti-ENG HPA011862 Polyclonal IHCWB 66 22Anti-FABP7 AMAb90595 Monoclonal IHCWB 89 90Anti-FABP7 HPA028825 Polyclonal IHCWB 89 90Anti-FLT1 AMAb90704 Monoclonal IHCWB 80 82Anti-GAP43 HPA015600 Polyclonal IHC ICC-IF 71 70Anti-GLI3 HPA005534 Polyclonal IHC 74 76Anti-MEF2C AMAb90727 Monoclonal IHCWBICC-IF 97 47Anti-MEF2C HPA005533 Polyclonal IHCWBICC-IF 97 47Anti-MKI67 (Ki67) HPA000451 Polyclonal IHCICC-IF 66 67Anti-NACC1 HPA021238 Polyclonal IHCICC-IF 91 89Anti-NES (Nestin) AMAb90556 Monoclonal IHCWBICC-IF 47 42Anti-NES (Nestin) HPA007007 Polyclonal IHCWB 47 42Anti-NKX2-2 HPA003468 Polyclonal IHCWB 96 97Anti-PAX6 HPA030775 Polyclonal IHCICC-IF 100 100Anti-PBK HPA005753 Polyclonal IHCWBICC-IF 91 94Anti-REST AMAb90740 Monoclonal IHCICC-IF 41 43Anti-RUNX1 HPA004176 Polyclonal IHCICC-IF 93 93Anti-RUNX2 AMAb90591 Monoclonal IHCWBICC-IF 100 81Anti-RUNX2 HPA022040 Polyclonal IHCWBICC-IF 100 81Anti-SATB2 AMAb90679 Monoclonal IHCWBICC-IF 100 100Anti-SATB2 HPA029543 Polyclonal IHCWBICC-IF 100 100Anti-SOX4 HPA029901 Polyclonal IHCICC-IF 100 39Anti-SOX6 HPA001923 Polyclonal IHCICC-IF 96 96Anti-SOX7 HPA009065 Polyclonal IHCWB 91 91Anti-SOX9 AMAb90795 Monoclonal IHCWBICC-IF 97 96Anti-SOX11 AMAb90502 Monoclonal IHCWB 82 82Anti-SOX11 HPA000536 Polyclonal IHCWB 82 82Anti-SOX30 HPA006159 Polyclonal IHCWB 68 70Anti-THY1 AMAb90844 Monoclonal IHCWB 64 68Anti-THY1 HPA003733 Polyclonal IHCICC-IF 64 68Anti-TM4SF2TSPAN7 HPA003140 Polyclonal IHCWB 96 96Anti-TM4SF2TSPAN7 AMAb90621 Monoclonal IHCWB 96 96Anti-VANGL1 AMAb90600 Monoclonal WBICC-IF 95 95Anti-VIM AMAb90516 Monoclonal IHCWB 99 99Anti-VIM HPA001762 Polyclonal IHCWBICC-IF 99 99
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Development
Products with enhanced validation for indicated application (see page 16)
15
At Atlas Antibodies we extensively validate our antibodies in IHC WB and ICC-IF Enhanced Validation is performed as an additional layer of security in an application and context-specific manner Enhanced validation offers increased security of antibody specificity in a defined context This is ensured by using the most relevant validation method for each combination of protein sample and application Enhanced Validation follows the guidelines proposed by the International Working Group for Antibody Validation (IWGAV)
ENHANCED VALIDATION
GENETIC VALIDATION Antibody specificity is confirmed by downregulating the target protein on a genetic level using siRNA
ORTHOGONAL VALIDATIONThe antibody is validated by comparing the results with a non-antibody based method across multiple samples
VALIDATION BY INDEPENDENT ANTIBODIESThe antibody specificity is demonstrated by comparing two antibodies targeting different regions of the same protein
RECOMBINANT EXPRESSION VALIDATIONThe antibody binding is verified using and over-expressed or tagged version of the target protein
MIGRATION CAPTURE MS VALIDATIONThe staining pattern and the protein size detected by the antibody is compared with results obtained by a capture Mass Spectrometry (MS) method
16
Triple A PolyclonalsTM are rabbit polyclonal primary antibodies developed within the Human Protein Atlas project IHC characterization data from 44 normal and 20 cancer tissues is available on the Human Protein Atlas portal
PrecisA MonoclonalsTM are mouse monoclonal primary antibodies developed against a number of carefully selected targets Clones are selected to recognize only unique non-overlapping epitopes and isotypes
Atlas Antibodies ABVoltavaumlgen 13SE-168 69 BrommaSwedenatlasantibodiescom
Phone +46(0)8 54 59 58 50contactatlasantibodiescomorderatlasantibodiescomsupportatlasantibodiescom
Atlas Antibodies logo Triple A Polyclonals PrecisA Monoclonals PrEST Antigen and QPrEST are trademarks or registered trademarks of Atlas Antibodies AB All other trademarks are the property of their respective owners
Products are for research use only Not for use in diagnostic procedures copy Atlas Antibodies AB 2020 008_
Neu
ro M
arke
r Pan
el _
Rev
03_2
0121
5_S
fN E
ditio
n
NEW PRODUCTS
Microglia markers
Astroc
ytes markers
Oligo
dendrocytes markers
NEUROINFLAMMATION
NEW MONOCLONAL ANTIBODIESNEW MONOCLONAL ANTIBODIESRead about our newly released antibodies markers on pages 6-7 3737
INDEXpage 3 ANTIBODIES FOR NEUROSCIENCE page 4 WHY ATLAS ANTIBODIES page 6 NEUROINFLAMMATION - SELECTED MARKERS page 8 SYNAPTIC SIGNALING - SELECTED MARKERS page 10 NEURAL LINEAGE- SELECTED MARKERS page 12 NEURODEGENERATIVE DISEASES - SELECTED MARKERS page 14 DEVELOPMENT - SELECTED MARKERS page 16 ENHANCED VALIDATION
Antibodies are valuable tools in the ongoing scientific effort to gain more knowledge about the human brain to understand the fundamental and emergent properties of neurons and neural circuits and to identify diseases such as neurological brain disorders Therefore there is a necessary need for highly-characterized and specific antibodies for neuroscience research
Our neuroscience catalog features a wide range of primary monoclonal and polyclonal antibodies for use in research designed to recognize the diverse neurochemical cell types in rodent and human nervous systems validated in cell lines rodents and human tissues
Our antibodies are validated for use in IHC WB and ICC-IF
ANTIBODIES FOR NEUROSCIENCE
ImmunofluorescenceWestern blot
Im
munohistochemistry3
VERY RELIABLE ANTIBODIESAtlas Antibodies is the original manufacturer of over 21000 primary antibodies targeting the majority of human proteins Our Triple A PolyclonalsTM and PrecisA MonoclonalsTM antibodies are available worldwide Building on our heritage with the Human Protein Atlas project we provide highly validated reagents that enable leading research in biology diagnostics and medicine for the purpose of understanding and improving human health
CREATED BY THE HUMAN PROTEIN ATLASTriple A Polyclonals are developed within the prestigious Human Protein Atlas project Our antibodies are validated in all major human tissues and organs and 20 cancer tissues Each antibody is supported by over 500 staining images
VALIDATED BY ENHANCED VALIDATIONWe take great care to validate our antibodies in IHC WB and ICC-IF As an additional layer of security we perform Enhanced Validation By using 5 different enhanced validation methods we validate our antibodies for each combination of protein sample and application
EVIDENCED BY SCIENCEOur antibodies are used all over the world and referenced in 1000s of scientific peer-reviewed papers
4
WHY ATLAS ANTIBODIES
The Human Protein Atlas
Atlas Antibodies
Learn more
NEUROINFLAMMATIONNeuroinflammation broadly defines the collective reactive immune response in the brain and spinal cord in response to injury and disease Inflammation in the central nervous system (CNS) is commonly associated with various degrees of tissue damage such as loss of myelin and neurons
The neuroinflammatory process is complex and involves disruption of the blood-brain barrier peripheral leukocyte infiltration edema and gliosis The inflammatory response is characterized by a host of cellular and molecular aberrations within the brain
Neuroinflammation arises locally within the CNS through phenotypic changes of different non-neuronal cell types in the brain such as microglia oligodendrocytes and astrocytes causing the release of different cytokines and chemokines and finally recruitment and infiltration of peripheral blood cells mainly T-and B-cells into the brain parenchyma
Neuroinflammatory processes are the key causative factors behind the brain and spinal cord injury This is true not only for acute brain trauma and hypoxic-ischemic brain damage following stroke but also for chronic infection and neurodegenerative diseases such as Alzheimerrsquos disease (AD) amyotrophic lateral sclerosis (ALS) Lewy body dementia (LBD) and multiple sclerosis (MS) In addition local peritumoral inflammation plays a role in the clinical progression and malignancy of glioblastomas the most aggressive primary brain tumors
Ant
i-GFA
P Anti-MBP Anti-P2RY12
Anti-
HLA-DRA antibody
Anti-
HLA-DRA Anti-GFAP
Anti-HLA-DRA (AMAb91674) shows microglial cells in red Anti-GFAP (AMAb91033) shows
astrocytes in green in Alzheimerrsquos disease cerebral cortex
Anti-P2RY12 (HPA014518) shows microglial cells in green Anti-GFAP(AMAb91033) shows
astrocytes in blue Anti-MBP (AMAb91062) shows myelinated processes in red in
Alzheimerrsquos disease cerebral cortex
Anti-HLA-DRA monoclonal antibody (AMAb91674) shows strong positivity in
microglial cells in Alzheimerrsquos disease cerebral cortex
6
New PrecisA MonoclonalsTM for Neuroinflammation
Products with enhanced validation for indicated application (see page 16)
7
Product Name
Product Number Clonality Validated
ApplicationsAnti-AGER AMAb91634 Monoclonal IgG2a IHC
Anti-AGER AMAb91635 Monoclonal IgG1 IHC
Anti-AIF1 AMAb91671 Monoclonal IgG2b IHC
Anti-AIF1 AMAb91672 Monoclonal IgG1 IHC
Anti-AMIGO3 AMAb91677 Monoclonal IgG1 WB
Anti-ANO2 AMAb91641 Monoclonal IgG1 IHC
Anti-ANO2 AMAb91642 Monoclonal IgG1 IHC
Anti-CD163 AMAb91646 Monoclonal IgG1 IHCWB
Anti-CD163 AMAb91648 Monoclonal IgG1 IHC
Anti-CRYAB AMAb91661 Monoclonal IgG1 IHC WB ICC-IF
Anti-CRYAB AMAb91662 Monoclonal IgG2b IHC WB
Anti-CXCL13 AMAb91629 Monoclonal IgG1 IHC
Anti-DYSF AMAb91667 Monoclonal IgG1 IHC WB
Anti-GAP43 AMAb91664 Monoclonal IgG2b IHC
Anti-GAP43 AMAb91665 Monoclonal IgG1 IHC
Anti-GAP43 AMAb91666 Monoclonal IgG2a IHC
Anti-GPR17 AMAb91624 Monoclonal IgG1 IHC WB
Anti-GZMB AMAb91650 Monoclonal IgG1 IHC
Anti-HLA-DRA AMAb91673 Monoclonal IgG1 IHC WB
Anti-HLA-DRA AMAb91674 Monoclonal IgG2a IHC WB
Anti-HLA-DRA AMAb91675 Monoclonal IgG2b IHC WB
Anti-IL17A AMAb91615 Monoclonal IgG1 IHC
Anti-IL17RA AMAb91617 Monoclonal IgG2b WB
Anti-IL17RA AMAb91619 Monoclonal IgG1 WB
Anti-IL7 AMAb91684 Monoclonal IgG1 ICC-IF
Anti-ITGA4 AMAb91699 Monoclonal IgG2b WB
Anti-MS4A1 AMAb91636 Monoclonal IgG2a IHC WB
Anti-OPALIN AMAb91685 Monoclonal IgG1 IHC
Anti-OPALIN AMAb91686 Monoclonal IgG2a IHC
Anti-PLP1 AMAb91639 Monoclonal IgG1 IHC
Anti-RGMA AMAb91702 Monoclonal IgG2b WB
Anti-S100A8 AMAb91689 Monoclonal IgG1 IHC
Anti-S100A9 AMAb91690 Monoclonal IgG2a IHC
Anti-SERPINA3 AMAb91655 Monoclonal IgG1 IHC
Anti-SPP1 AMAb91653 Monoclonal IgG1 IHC
Anti-TLR2 AMAb91631 Monoclonal IgG1 WB
Anti-TRPM4 AMAb91693 Monoclonal IgG1 IHC WB ICC-IF
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Anti-AGER HPA064436 Polyclonal WB ICC-IF 41 41
Anti-AIF1 HPA049234 Polyclonal IHC 84 84
Anti-AIF1 HPA062949 Polyclonal ICC-IF 86 83
Anti-ANO2 HPA057499 Polyclonal IHC 84 85
Anti-CD163 HPA046404 Polyclonal IHC ICC-IF 51 30
Anti-CD163 HPA051974 Polyclonal IHC 75 83
Anti-CRYAB HPA057100 Polyclonal IHC 98 98
Anti-DYSF HPA017071 Polyclonal IHC ICC-IF 96 96
Anti-DYSF HPA021945 Polyclonal IHC 88 88
Anti-GAP43 HPA013392 Polyclonal IHC ICC-IF 88 86
Anti-GAP43 HPA015600 Polyclonal IHC ICC-IF 71 70
Anti-GPR17 HPA029766 Polyclonal IHC 92 92
Anti-GZMB HPA003418 Polyclonal IHC WB 63 63
Anti-HLA-DRA HPA050162 Polyclonal IHC 54 69
Anti-HLA-DRA HPA053176 Polyclonal IHC 41 83
Anti-IL7 HPA019590 Polyclonal IHC 46 46
Anti-ITGA4 HPA074961 Polyclonal ICC-IF 90 89
Anti-MS4A1 HPA014341 Polyclonal IHC WB ICC-IF 73 77
Anti-MS4A1 HPA014391 Polyclonal IHC WB 61 64
Anti-OPALIN HPA014372 Polyclonal IHC 72 75
Anti-PLP1 HPA004128 Polyclonal IHC 100 100
Anti-RGMA HPA039880 Polyclonal ICC-IF 96 96
Anti-RGMA HPA044222 Polyclonal ICC-IF 87 87
Anti-S100A8 HPA024372 Polyclonal IHC WB ICC-IF 56 60
Anti-SERPINA3 HPA000893 Polyclonal IHC WB 61 61
Anti-SERPINA3 HPA002560 Polyclonal IHC WB 60 59
Anti-SPP1 HPA027541 Polyclonal IHC WB ICC-IF 56 53
Anti-TRPM4 HPA041169 Polyclonal IHC ICC-IF 93 91
NEW PRODUCTS
Selected Triple A PolyclonalsTM for Neuroinflammation
Products with enhanced validation for indicated application (see page 16)
Synaptic signaling is cell-cell communication that occurs at the synaptic level Cells communicate with each other via direct contact (juxtacrine signaling) over short distances (paracrine signaling) or vast distances and scales (endocrine signaling) Other cells form gap junctions that connect their cytoplasm to the cytoplasm of adjacent cells
When the sending neuron fires an electrical impulse travels along the axon As soon as the impulse reaches the synapse it triggers the release of neurotransmitters
The predominant excitatory neurotransmitter in the brain is glutamate (GLU) The inhibitory neurotransmitters GABA (in the brain) and glycine (in the brainstem and spinal cord) are common amino acids that participate in multiple metabolic cycles
Calcium (Ca2+) signaling in neurons is critical in cell signalingIt defines the ability of cells to release neurotransmitters thus regulating the intracellular signaling pathways Calbindin 2 (also known as calretinin) is one of the Ca2+-binding proteins involved in Ca2+ signaling that facilitates cytosolic Ca2+ diffusion without interfering with Ca2+ signaling
SYNAPTIC SIGNALING
A
nti-CALB2 antibody
An
ti-mGluR1 antibody
A
nti-VGAT antibody
Anti-VGAT monoclonal antibody (AMAb91043) shows strong immunoreactivity in GABAergic
fibers in the rat globus pallidus in green
Anti-CALB2 polyclonal antibody (HPA007305) shows the labeling in green of mouse
hippocampus Note the strong labeling in the dentate gyrus
Anti-MGLUR1 polyclonal antibody (HPA015701) strongly labels cortical perikarya in human
cerebral cortex tissue in brown
8
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Synaptic Signaling
9
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Anti-ATF2 HPA022134 Polyclonal IHC WB ICC-IF 99 99
Anti-ATF3 AMAb90909 Monoclonal IHC ICC-IF 92 92
Anti-ATF3 HPA001562 Polyclonal IHC ICC-IF 92 92
Anti-ATP1B1 HPA012911 Polyclonal IHC WB 93 93
Anti-ATP1B2 HPA010698 Polyclonal IHC 96 88
Anti-CAMK2B HPA026307 Polyclonal IHC WB 96 96
Anti-CALB1 HPA023099 Polyclonal IHC WB ICC-IF 98 99
Anti-CALB2 HPA007306 Polyclonal IHC WB ICC-IF 100 100
Anti-CALB2 HPA007305 Polyclonal IHC WB ICC-IF 100 100
Anti-CAMK2D HPA026281 Polyclonal IHC 100 97
Anti-C-FOS HPA018531 Polyclonal IHC ICC-IF 94 94
Anti-CHAT AMAb91130 Monoclonal IHC ICC-IF 96 96
Anti-CHAT HPA048547 Polyclonal IHC 96 96
Anti-CHRM1 (M1 mAChR) HPA014101 Polyclonal IHC 98 97
Anti-CHRM2 (M2 AChR) HPA029795 Polyclonal IHC 88 86
Anti-CLIC4 HPA008019 Polyclonal IHC WB 98 97
Anti-CREB1 HPA019150 Polyclonal IHC WB ICC-IF 100 100
Anti-DDC AMAb91089 Monoclonal IHCWB 90 88
Anti-DDC HPA017742 Polyclonal IHCWBICC-IF 90 88
Anti-EAAC1 HPA020086 Polyclonal IHC WB 77 79
Anti-EAAT2 HPA009172 Polyclonal IHC 87 89
Anti-GABRA3 HPA000839 Polyclonal IHCWB 91 93
Anti-GABRB1 HPA051297 Polyclonal IHC 97 100
Anti-GABRG1 HPA035622 Polyclonal IHC 96 94
Anti-GAD1 (GAD67) AMAb91076 Monoclonal IHCWB 94 94
Anti-GAD1 HPA058412 Polyclonal IHCWB 94 94
Anti-GAD2 AMAb91048 Monoclonal IHCWB 84 88
Anti-GAD2 HPA044637 Polyclonal IHC 84 88
Anti-GAT1 HPA013341 Polyclonal IHC WB 98 98
Anti-GAT3 HPA037981 Polyclonal IHCWB 85 87
Anti-GLUR2 (AMPA2) HPA008441 Polyclonal IHC 100 100
Anti-HTR2A HPA014011 Polyclonal IHC 95 97
Anti-KCC4 HPA041652 Polyclonal IHCWB 84 82
Anti-KCNJ5 (KIR34) HPA017353 Polyclonal IHCWB 89 89
Anti-KIF11 HPA010568 Polyclonal IHCWB 88 83
Anti-KIF17 HPA032085 Polyclonal IHCICC-IF 85 82
Anti-KIF18A HPA039312 Polyclonal IHCWBICC-IF 80 82
Anti-KIF1C HPA024602 Polyclonal IHCWB 81 83
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Anti-KIF26B HPA028562 Polyclonal IHCICC-IF 88 80
Anti-KIF4A (KIF4A amp B) HPA034745 Polyclonal IHCWBICC-IF 64 63
Anti-KIF5A HPA004469 Polyclonal IHCWB 91 88
Anti-KIF5C HPA035210 Polyclonal IHCWB 100100
Anti-KIF7 HPA043145 Polyclonal IHC 69 69
Anti-KIFAP3 HPA023742 Polyclonal IHC 100 100
Anti-MAPK1 (ERK) HPA030069 Polyclonal IHCWB 100 100
Anti-MAPK3 (ERK1) HPA005700 Polyclonal IHCWB 98 98
Anti-MGLUR1 HPA015701 Polyclonal IHC 80 80
Anti-MGLUR8 HPA051481 Polyclonal IHC 95 90
Anti-NCS1 HPA019713 Polyclonal IHCWBICC-IF 100 100
Anti-PRKCA HPA006563 Polyclonal IHCWBICC-IF 99 99
Anti-PRKCH HPA053709 Polyclonal IHCICC-IF 97 64
Anti-PNMT HPA051005 Polyclonal IHC 89 92
Anti-PRKACB (PKACB) HPA029754 Polyclonal IHC 73 76
Anti-PRKCZ HPA021851 Polyclonal IHCWB ICC-IF 94 94
Anti-RAB3A HPA003160 Polyclonal IHC 99 99
Anti-RAP1GAP HPA001922 Polyclonal IHC 92 91
Anti-SLC17A7 (VGLUT1) AMAb91041 Monoclonal IHCWB 94 94
Anti-SLC22A2 HPA008567 Polyclonal IHC 84 77
Anti-SLC32A1 (VGAT) HPA058859 Polyclonal IHC 95 93
Anti-SLC6A2 (NET) AMAb91116 Monoclonal IHC -
Anti-SLC6A3 (DAT) AMAb91125 Monoclonal IHC 85 85
Anti-SNAP25 HPA001830 Polyclonal IHCWB 100 100
Anti-SNAP29 HPA031823 Polyclonal IHC 89 92
Anti-SST (SOM) HPA019472 Polyclonal IHCWB 98 98
Anti-STXBP1 HPA008209 Polyclonal IHCWBICC-IF 100 100
Anti-STXBP6 HPA003552 Polyclonal IHCWB 99 99
Anti-SYNGR1 HPA029673 Polyclonal IHC ICC-IF 90 88
Anti-SYNPR HPA061671 Polyclonal IHC 97 97
Anti-SYP HPA002858 Polyclonal IHCWB 83 83
Anti-SYT1 HPA008394 Polyclonal IHCWB 100 100
Anti-SYT12 HPA011006 Polyclonal IHC 96 98
Anti-SYT13 HPA046224 Polyclonal IHC 96 93
Anti-SYT16 HPA004199 Polyclonal IHCWB 95 95
Anti-TGFA HPA042297 Polyclonal IHC 93 93
Anti-TH AMAb91112 Monoclonal IHC 88 88
Anti-TH HPA061003 Polyclonal IHC WB 88 88
Anti-TPH2 (NTPH) AMAb91108 Monoclonal IHC 100 100
Anti-VAMP4 HPA050418 Polyclonal IHCICC-IF 100 100
Anti-VAMP7 HPA036733 Polyclonal IHCICC-IF 98 93
Products with enhanced validation for indicated application (see page 16)
NEURAL LINEAGE
A
nti-GFAP antibody
Anti-C
NP antibody
Anti-N
EFM antibodyDuring central nervous system development neural stem cells (NSCs) proliferate and divide to generate a progeny that differentiate into neurons astrocytes oligodendrocytes and different glial cells
NSCs self-renewal and differentiation are regulated by a precise temporal sequence of growth factors intracellular signaling and transcription factor expression NSCs and their differentiated progeny can be identified by the expression of a unique combination of cell surface markers and transcription factors
The glial fibrillary acidic protein (GFAP) is an intermediate filament (IF) protein and a well-known marker for astrocytes
Oligodendrocytes can convert extracellular 2rsquo3rsquo-cAMP to adenosine As CNPase is the predominant enzyme involved in this conversion the cyclic nucleotide phosphodiesterase (CNP) is widely used as an oligodendrocyte marker
Neurons express the neurofilament medium (NFEM) polypeptide other intermediate filament proteins including nestin synemin syncoilin and vimentin
Anti-GFAP polyclonal antibody (HPA056030) shows strong immunoreactivity in the
human cerebellum in brown
Anti-NEFM polyclonal antibody (HPA022845) shows positivity in a subset of neuronal cells in
human cerebral cortex in brown
Anti-CNP polyclonal antibody (HPA023266) in the rat cerebral cortex is shown in magenta
10
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Neural Lineage
Products with enhanced validation for indicated application (see page 16)
11
TargetCells
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Astrocytes Anti-GFAP AMAb91033 Monoclonal IHC WB 98 100
Astrocytes Anti-GFAP HPA056030 Polyclonal IHCWB ICC-IF 98 100
Astrocytes Anti-GLUL AMAb91101 Monoclonal IHC WB 95 53
Astrocytes Anti-GLUL AMAb91102 Monoclonal IHC WB 95 92
Astrocytes Anti-GLUL AMAb91103 Monoclonal IHC WB 95 92
Astrocytes Anti-GLUL HPA007316 Polyclonal IHCWB 95 53
Astrocytes Anti-S100B AMAb91038 Monoclonal IHC WB 99 98
Astroglia Anti-EZR AMAb90976 Monoclonal IHCWBICC-IF 93 93
Astroglia Anti-EZR HPA021616 Polyclonal IHCWBICC-IF 93 93
Adhesion molecule Anti-ICAM5 HPA009083 Polyclonal IHC 85 86
Adhesion molecule Anti-MCAM HPA008848 Polyclonal IHC WB 75 73
Adhesion molecule Anti-NCAM2 HPA030900 Polyclonal IHC 89 91
Adhesion molecule Anti-NLGN1 HPA006680 Polyclonal IHCWB 98 98
Calcium binding protein Anti-CALB1 (CB) HPA023099 Polyclonal IHCWBICC-IF 98 99
Calcium binding protein Anti-CALB2 (CR) HPA007305 Polyclonal IHCWBICC-IF 100 100
Calcium binding proteins Anti-NECAB1 AMAb90801 Monoclonal IHCWB 98 98
Calcium binding protein Anti-NECAB1 HPA023629 Polyclonal IHCWB 98 98
Calcium binding protein Anti-NECAB2 AMAb90808 Monoclonal IHC 85 84
Calcium binding protein Anti-NECAB2 HPA013998 Polyclonal IHCICC-IF 98 97
Calcium binding protein Anti-SCGN AMAb90630 Monoclonal IHCWB 96 96
Calcium binding protein Anti-SCGN HPA006641 Polyclonal IHC 96 96
Calcium binding protein Anti-S100B HPA015768 Polyclonal IHCWBICC-IF 99 98
Oligodendrocytes Anti-MOG AMAb91066 Monoclonal IHC WB 91 89
Oligodendrocytes Anti-MOG AMAb91067 Monoclonal IHC WB 91 89
Oligodendrocytes Anti-MOG HPA021873 Polyclonal IHC 91 89
Oligodendrocytes Anti-CNP AMAb91068 Monoclonal IHC WB 76 77
Oligodendrocytes Anti-CNP AMAb91069 Monoclonal IHC WB 76 77
Oligodendrocytes Anti-CNP AMAb91072 Monoclonal IHC WB ICC-IF 76 77
Oligodendrocytes Anti-CNP HPA023280 Polyclonal IHCWBICC-IF 76 77
Progenitors Anti-PBK HPA005753 Polyclonal IHCWBICC-IF 91 94
Progenitors Anti-MKI67 (Ki67) AMAb90870 Monoclonal IHC ICC-IF 68 68
Progenitors Anti-MKI67 (Ki67) HPA000451 Polyclonal IHCICC-IF 66 67
TargetCells
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Cytoskeleton Anti-ACTN1 HPA006035 Polyclonal IHCWB 98 99
Cytoskeleton Anti-ACTN4 HPA001873 Polyclonal IHCWBICC-IF 99 98
Cytoskeleton Anti-INA HPA008057 Polyclonal IHC WB ICC-IF 83 84
Cytoskeleton Anti-MAP1A HPA039064 Polyclonal IHC 60 52
Cytoskeleton Anti-MAP1B HPA022275 Polyclonal IHCWB ICC-IF 85 86
Cytoskeleton Anti-MAP2 HPA008273 Polyclonal IHC WB ICC-IF 96 96
Cytoskeleton Anti-MYO5A HPA001356 Polyclonal IHCICC-IF 99 98
Cytoskeleton Anti-NEFM (NF160) HPA022845 Polyclonal IHCWBICC-IF 98 98
Microglia Anti-AIF HPA049234 Polyclonal IHC 84 84
Microglia Anti-CD68 AMAb90874 Monoclonal IHCWB 76 76
Microglia Anti-CD68 HPA048982 Polyclonal IHC WB 76 76
Microglia Anti-ITGAM (CD11b) AMAb90911 Monoclonal IHC WB 67 68
Microglia Anti-PTPRC AMAb90518 Monoclonal IHCWB 35 37
Microglia Anti-PTPRC HPA000440 Polyclonal IHCWB 35 37
Microglia Anti-TMEM119 AMAb91528 Monoclonal IHCWB ICC-IF 52 46
Microglia Anti-TMEM119 HPA051870 Polyclonal IHC 52 46
Neurons Anti-NEFM (NF160) AMAb91027 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91028 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91029 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91030 Monoclonal IHC WB 98 98
Neurons Anti-NEFH (NF200) AMAb91025 Monoclonal IHC WB 98 98
Neurons Anti-NEFH (NF200) HPA061615 Polyclonal IHC 88 94
Neurons Anti-UCHL1 AMAb91145 Monoclonal IHC WB ICC-IF 97 97
Neurons Anti-UCHL1 HPA005993 Polyclonal IHCWBICC-IF 97 97
Neurons Anti-VIM AMAb90516 Monoclonal IHCWB 99 99
Neurons Anti-VIM HPA001762 Polyclonal IHCWBICC-IF 99 99
Schwann cells Anti-MBP AMAb91062 Monoclonal IHC WB ICC-IF 97 97
Schwann cells Anti-MBP AMAb91063 Monoclonal IHC WB ICC-IF 97 97
Schwann cells Anti-MBP AMAb91064 Monoclonal IHC WB 97 97
Anti-I
TM2B antibodyNeurodegenerative diseases such as Amyotrophic Lateral Sclerosis Parkinsonrsquos Alzheimerrsquos and Huntingtonrsquos diseases occur as a result of the progressive loss of structure and function in neurons leading to the death of neurons
Age remains the primary risk factor for almost all the neurodegenerative disorders thus interfering with age-related molecular mechanisms or pathways might be an avenue to develop new therapeutics
The choline acetyltransferase (ChAT) is an enzyme responsible for catalyzing the synthesis of acetylcholine and is expressed in the majority of cholinergic neurons Hence ChAT immunoreactivity is used to detect cognitive decline in several neurodegenerative disorders
Integral membrane protein 2B (ITM2B) is a protein that in humans is encoded by the ITM2B gene This gene located on chromosome 13 is connected to familial dementia causing amyloid and pre-fibrillar effects similar to those seen in Alzheimerrsquos
The expression of the ubiquitin-carboxyl-terminal hydrolase isozyme L1 (UCHL1) present both in the neuronal cells of the central and peripheral nervous system is down-regulated in Parkinsonrsquos and Alzheimerrsquos disease patients
NEURODEGENERATIVE DISEASES
Anti-C
HAT antibody
Anti-U
CHL1 antibody
Anti-CHAT monoclonal antibody (AMAb91130) shows strong immunoreactivity in the
acetylcholine neurons in the rat diagonal band of Broca in green
Anti-ITM2B polyclonal antibody (HPA029292) targeting Integral membrane protein 2B strongly
labels Golgi apparatus in A-431 cells in green
Anti-UCHL1 monoclonal antibody (AMAb91145) in the mouse trigeminal ganglion show strong
immunoreactrivity in primary sensory neurons in green
12
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for NeurodegenerationProduct Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-ADAR AMAb90535 Monoclonal IHCWB ICC-IF 86 85
Anti-ADAR HPA003890 Polyclonal IHCWBICC-IF 86 85
Anti-AIMP1 HPA018476 Polyclonal IHCWBICC-IF 96 97
Anti-AKT1 AMAb90834 Monoclonal WB ICC-IF 97 97
Anti-AOX1 HPA040199 Polyclonal IHC 84 86
Anti-APBA3 HPA045577 Polyclonal IHC 70 68
Anti-APBB2 HPA023542 Polyclonal IHCWBICC-IF 85 83
Anti-APBB3 HPA005571 Polyclonal IHCICC-IF 84 84
Anti-APOA4 AMAb90769 Monoclonal IHC WB 65 28
Anti-APP HPA001462 Polyclonal IHCWB 95 95
Anti-AQP4 AMAb90537 Monoclonal IHCWB 93 92
Anti-AQP4 HPA014784 Polyclonal IHCWB ICC-IF 93 92
Anti-ATF2 HPA022134 Polyclonal IHCWBICC-IF 99 99
Anti-ATF3 AMAb90909 Monoclonal IHCICC-IF 92 92
Anti-ATF3 HPA001562 Polyclonal IHCICC-IF 92 92
Anti-ATRX AMAb90784 Monoclonal IHC WBICC-IF 96 97
Anti-ATXN1 HPA008335 Polyclonal IHC 81 81
Anti-ATXN2 HPA018295 Polyclonal IHCWBICC-IF 90 91
Anti-C3 HPA020432 Polyclonal IHC 78 23
Anti-CASP3 HPA002643 Polyclonal IHCWBICC-IF 84 88
Anti-CD4 AMAb90754 Monoclonal IHCWB 55 57
Anti-CD40 AMAb90905 Monoclonal IHCWB 58 54
Anti-CHAT AMAb91130 Monoclonal IHCICC-IF 96 96
Anti-CHAT HPA048547 Polyclonal IHC 96 96
Anti-CHGA AMAb90525 Monoclonal IHCWBICC-IF 62 64
Anti-COX1 HPA002834 Polyclonal IHCWBICC-IF 93 90
Anti-COX2PTGS2 HPA001335 Polyclonal IHCICC-IF 88 88
Anti-CTSB HPA018156 Polyclonal IHCICC-IF 79 79
Anti-CTSD HPA003001 Polyclonal IHCWB 86 86
Anti-CXorf27 HPA003356 Polyclonal IHC 47 46
Anti-FBXO7 (PARK15) HPA032114 Polyclonal IHC 78 81
Anti-FUS AMAb90549 Monoclonal IHC WB ICC-IF 91 91Anti-GSK3B HPA028017 Polyclonal IHCWBICC-IF 100 100Anti-HIP1 HPA017964 Polyclonal IHCWB 79 77Anti-HTRA2 (PARK13) HPA027366 Polyclonal IHCWB 57 63Anti-ITGAM (CD11b) AMAb90911 Monoclonal IHCWB 67 68
Anti-ITM2B HPA029292 Polyclonal IHC ICC-IF 95 96Anti-LRP2 HPA005980 Polyclonal IHC 78 36
Products with enhanced validation for indicated application (see page 16)
13
Product Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-NFKB1 HPA027305 Polyclonal IHCWBICC-IF 60 62
Anti-OPTN HPA003360 Polyclonal IHCWBICC-IF 64 68
Anti-PADI4 HPA017007 Polyclonal IHCWB 66 69
Anti-PARK7 HPA004190 Polyclonal IHCWB 89 90
Anti-PFN1 AMAb91181 Monoclonal IHCWB 87 87
Anti-PHGDH AMAb90786 Monoclonal IHCWBICC-IF 99 99
Anti-PHGDH HPA021241 Polyclonal IHCWBICC-IF 99 99
Anti-PRNP HPA042754 Polyclonal IHC 91 91
Anti-PSEN1 HPA030760 Polyclonal IHC 82 81
Anti-RHOT1 AMAb90852 Monoclonal IHCWB 100 100
Anti-RHOT1 HPA010687 Polyclonal IHC 100 100
Anti-S100A8 HPA024372 Polyclonal IHCWBICC-IF 56 60
Anti-SERPINA3 HPA002560 Polyclonal IHCWB 60 59
Anti-SNCB HPA035876 Polyclonal IHCWB 97 97
Anti-SOD1 HPA001401 Polyclonal IHCWBICC-IF 82 81
Anti-SOD2 HPA001814 Polyclonal IHCWB 88 87
Anti-THY1 AMAb90844 Monoclonal IHCWB 64 68
Anti-THY1 HPA003733 Polyclonal IHCICC-IF 64 68
Anti-TNFRSF21 HPA006746 Polyclonal IHCWB 86 85
Anti-TTR AMAb90649 Monoclonal IHC WB 81 83
Anti-UBE2K HPA028869 Polyclonal IHCICC-IF 100 100
Anti-UCHL1 HPA005993 Polyclonal IHCWBICC-IF 97 97
Anti-USP46 AMAb90722 Monoclonal WBICC-IF 100 99
Anti-VPS26A AMAb90967 Monoclonal IHC WBICC-IF 96 100
Anti-WHSC1 AMAb90851 Monoclonal IHCWBICC-IF 91 91
Anti-WHSC1 HPA015801 Polyclonal IHCICC-IF 91 91
Anti-VWF AMAb90928 Monoclonal IHCWB 80 80
Anti-VWF HPA002082 Polyclonal IHC 82 78
The development of the central nervous system includes the development of the brain spinal cord optic and auditory systems
Within the neural tube stem cells generate the two major classes of cells that make the majority of the nervous system neurons and glia Both these classes of cells differentiate into many different types generated with highly specialized functions and shapes
The development of the peripheral and central nervous systems begins early in embryogenesis and can be traced through the use of lineage antibodies specific to each stage of neuronal development
The (SOX) family of transcription factors are well-established regulators of cell fate decisions during development
Vimentin (VIM) is a type III intermediate filament protein expressed in mesenchymal cells It is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both healthy development and metastatic progression
The cell surface glycoprotein Thy1 (CD90) is expressed on many mammalian cell types including neurons and serves as a human mesenchymal stem cell (MSC) marker
DEVELOPMENT
A
nti-VIM antibody
A
nti-SOX4 antibody
Anti-T
HY1 antibody
Anti-VIM polyclonal antibody (HPA001762) immunofluorescent staining of human cell line
ASC TERT1 shows localization to intermediate filaments in green
Anti-SOX4 monoclonal antibody (AMAb913802) immunofluorescent staining in mouse
hippocampus showing immunoreactivity in a subset of neurons in the granule cell layer of
the dentate gyrus in brown
Anti-THY1 polyclonal antibody (HPA003733) staining of human cerebral cortex shows
localization to neuropils in brown
Product Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-ENG AMAb90925 Monoclonal IHC 66 22Anti-ENG HPA011862 Polyclonal IHCWB 66 22Anti-FABP7 AMAb90595 Monoclonal IHCWB 89 90Anti-FABP7 HPA028825 Polyclonal IHCWB 89 90Anti-FLT1 AMAb90704 Monoclonal IHCWB 80 82Anti-GAP43 HPA015600 Polyclonal IHC ICC-IF 71 70Anti-GLI3 HPA005534 Polyclonal IHC 74 76Anti-MEF2C AMAb90727 Monoclonal IHCWBICC-IF 97 47Anti-MEF2C HPA005533 Polyclonal IHCWBICC-IF 97 47Anti-MKI67 (Ki67) HPA000451 Polyclonal IHCICC-IF 66 67Anti-NACC1 HPA021238 Polyclonal IHCICC-IF 91 89Anti-NES (Nestin) AMAb90556 Monoclonal IHCWBICC-IF 47 42Anti-NES (Nestin) HPA007007 Polyclonal IHCWB 47 42Anti-NKX2-2 HPA003468 Polyclonal IHCWB 96 97Anti-PAX6 HPA030775 Polyclonal IHCICC-IF 100 100Anti-PBK HPA005753 Polyclonal IHCWBICC-IF 91 94Anti-REST AMAb90740 Monoclonal IHCICC-IF 41 43Anti-RUNX1 HPA004176 Polyclonal IHCICC-IF 93 93Anti-RUNX2 AMAb90591 Monoclonal IHCWBICC-IF 100 81Anti-RUNX2 HPA022040 Polyclonal IHCWBICC-IF 100 81Anti-SATB2 AMAb90679 Monoclonal IHCWBICC-IF 100 100Anti-SATB2 HPA029543 Polyclonal IHCWBICC-IF 100 100Anti-SOX4 HPA029901 Polyclonal IHCICC-IF 100 39Anti-SOX6 HPA001923 Polyclonal IHCICC-IF 96 96Anti-SOX7 HPA009065 Polyclonal IHCWB 91 91Anti-SOX9 AMAb90795 Monoclonal IHCWBICC-IF 97 96Anti-SOX11 AMAb90502 Monoclonal IHCWB 82 82Anti-SOX11 HPA000536 Polyclonal IHCWB 82 82Anti-SOX30 HPA006159 Polyclonal IHCWB 68 70Anti-THY1 AMAb90844 Monoclonal IHCWB 64 68Anti-THY1 HPA003733 Polyclonal IHCICC-IF 64 68Anti-TM4SF2TSPAN7 HPA003140 Polyclonal IHCWB 96 96Anti-TM4SF2TSPAN7 AMAb90621 Monoclonal IHCWB 96 96Anti-VANGL1 AMAb90600 Monoclonal WBICC-IF 95 95Anti-VIM AMAb90516 Monoclonal IHCWB 99 99Anti-VIM HPA001762 Polyclonal IHCWBICC-IF 99 99
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Development
Products with enhanced validation for indicated application (see page 16)
15
At Atlas Antibodies we extensively validate our antibodies in IHC WB and ICC-IF Enhanced Validation is performed as an additional layer of security in an application and context-specific manner Enhanced validation offers increased security of antibody specificity in a defined context This is ensured by using the most relevant validation method for each combination of protein sample and application Enhanced Validation follows the guidelines proposed by the International Working Group for Antibody Validation (IWGAV)
ENHANCED VALIDATION
GENETIC VALIDATION Antibody specificity is confirmed by downregulating the target protein on a genetic level using siRNA
ORTHOGONAL VALIDATIONThe antibody is validated by comparing the results with a non-antibody based method across multiple samples
VALIDATION BY INDEPENDENT ANTIBODIESThe antibody specificity is demonstrated by comparing two antibodies targeting different regions of the same protein
RECOMBINANT EXPRESSION VALIDATIONThe antibody binding is verified using and over-expressed or tagged version of the target protein
MIGRATION CAPTURE MS VALIDATIONThe staining pattern and the protein size detected by the antibody is compared with results obtained by a capture Mass Spectrometry (MS) method
16
Triple A PolyclonalsTM are rabbit polyclonal primary antibodies developed within the Human Protein Atlas project IHC characterization data from 44 normal and 20 cancer tissues is available on the Human Protein Atlas portal
PrecisA MonoclonalsTM are mouse monoclonal primary antibodies developed against a number of carefully selected targets Clones are selected to recognize only unique non-overlapping epitopes and isotypes
Atlas Antibodies ABVoltavaumlgen 13SE-168 69 BrommaSwedenatlasantibodiescom
Phone +46(0)8 54 59 58 50contactatlasantibodiescomorderatlasantibodiescomsupportatlasantibodiescom
Atlas Antibodies logo Triple A Polyclonals PrecisA Monoclonals PrEST Antigen and QPrEST are trademarks or registered trademarks of Atlas Antibodies AB All other trademarks are the property of their respective owners
Products are for research use only Not for use in diagnostic procedures copy Atlas Antibodies AB 2020 008_
Neu
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INDEXpage 3 ANTIBODIES FOR NEUROSCIENCE page 4 WHY ATLAS ANTIBODIES page 6 NEUROINFLAMMATION - SELECTED MARKERS page 8 SYNAPTIC SIGNALING - SELECTED MARKERS page 10 NEURAL LINEAGE- SELECTED MARKERS page 12 NEURODEGENERATIVE DISEASES - SELECTED MARKERS page 14 DEVELOPMENT - SELECTED MARKERS page 16 ENHANCED VALIDATION
Antibodies are valuable tools in the ongoing scientific effort to gain more knowledge about the human brain to understand the fundamental and emergent properties of neurons and neural circuits and to identify diseases such as neurological brain disorders Therefore there is a necessary need for highly-characterized and specific antibodies for neuroscience research
Our neuroscience catalog features a wide range of primary monoclonal and polyclonal antibodies for use in research designed to recognize the diverse neurochemical cell types in rodent and human nervous systems validated in cell lines rodents and human tissues
Our antibodies are validated for use in IHC WB and ICC-IF
ANTIBODIES FOR NEUROSCIENCE
ImmunofluorescenceWestern blot
Im
munohistochemistry3
VERY RELIABLE ANTIBODIESAtlas Antibodies is the original manufacturer of over 21000 primary antibodies targeting the majority of human proteins Our Triple A PolyclonalsTM and PrecisA MonoclonalsTM antibodies are available worldwide Building on our heritage with the Human Protein Atlas project we provide highly validated reagents that enable leading research in biology diagnostics and medicine for the purpose of understanding and improving human health
CREATED BY THE HUMAN PROTEIN ATLASTriple A Polyclonals are developed within the prestigious Human Protein Atlas project Our antibodies are validated in all major human tissues and organs and 20 cancer tissues Each antibody is supported by over 500 staining images
VALIDATED BY ENHANCED VALIDATIONWe take great care to validate our antibodies in IHC WB and ICC-IF As an additional layer of security we perform Enhanced Validation By using 5 different enhanced validation methods we validate our antibodies for each combination of protein sample and application
EVIDENCED BY SCIENCEOur antibodies are used all over the world and referenced in 1000s of scientific peer-reviewed papers
4
WHY ATLAS ANTIBODIES
The Human Protein Atlas
Atlas Antibodies
Learn more
NEUROINFLAMMATIONNeuroinflammation broadly defines the collective reactive immune response in the brain and spinal cord in response to injury and disease Inflammation in the central nervous system (CNS) is commonly associated with various degrees of tissue damage such as loss of myelin and neurons
The neuroinflammatory process is complex and involves disruption of the blood-brain barrier peripheral leukocyte infiltration edema and gliosis The inflammatory response is characterized by a host of cellular and molecular aberrations within the brain
Neuroinflammation arises locally within the CNS through phenotypic changes of different non-neuronal cell types in the brain such as microglia oligodendrocytes and astrocytes causing the release of different cytokines and chemokines and finally recruitment and infiltration of peripheral blood cells mainly T-and B-cells into the brain parenchyma
Neuroinflammatory processes are the key causative factors behind the brain and spinal cord injury This is true not only for acute brain trauma and hypoxic-ischemic brain damage following stroke but also for chronic infection and neurodegenerative diseases such as Alzheimerrsquos disease (AD) amyotrophic lateral sclerosis (ALS) Lewy body dementia (LBD) and multiple sclerosis (MS) In addition local peritumoral inflammation plays a role in the clinical progression and malignancy of glioblastomas the most aggressive primary brain tumors
Ant
i-GFA
P Anti-MBP Anti-P2RY12
Anti-
HLA-DRA antibody
Anti-
HLA-DRA Anti-GFAP
Anti-HLA-DRA (AMAb91674) shows microglial cells in red Anti-GFAP (AMAb91033) shows
astrocytes in green in Alzheimerrsquos disease cerebral cortex
Anti-P2RY12 (HPA014518) shows microglial cells in green Anti-GFAP(AMAb91033) shows
astrocytes in blue Anti-MBP (AMAb91062) shows myelinated processes in red in
Alzheimerrsquos disease cerebral cortex
Anti-HLA-DRA monoclonal antibody (AMAb91674) shows strong positivity in
microglial cells in Alzheimerrsquos disease cerebral cortex
6
New PrecisA MonoclonalsTM for Neuroinflammation
Products with enhanced validation for indicated application (see page 16)
7
Product Name
Product Number Clonality Validated
ApplicationsAnti-AGER AMAb91634 Monoclonal IgG2a IHC
Anti-AGER AMAb91635 Monoclonal IgG1 IHC
Anti-AIF1 AMAb91671 Monoclonal IgG2b IHC
Anti-AIF1 AMAb91672 Monoclonal IgG1 IHC
Anti-AMIGO3 AMAb91677 Monoclonal IgG1 WB
Anti-ANO2 AMAb91641 Monoclonal IgG1 IHC
Anti-ANO2 AMAb91642 Monoclonal IgG1 IHC
Anti-CD163 AMAb91646 Monoclonal IgG1 IHCWB
Anti-CD163 AMAb91648 Monoclonal IgG1 IHC
Anti-CRYAB AMAb91661 Monoclonal IgG1 IHC WB ICC-IF
Anti-CRYAB AMAb91662 Monoclonal IgG2b IHC WB
Anti-CXCL13 AMAb91629 Monoclonal IgG1 IHC
Anti-DYSF AMAb91667 Monoclonal IgG1 IHC WB
Anti-GAP43 AMAb91664 Monoclonal IgG2b IHC
Anti-GAP43 AMAb91665 Monoclonal IgG1 IHC
Anti-GAP43 AMAb91666 Monoclonal IgG2a IHC
Anti-GPR17 AMAb91624 Monoclonal IgG1 IHC WB
Anti-GZMB AMAb91650 Monoclonal IgG1 IHC
Anti-HLA-DRA AMAb91673 Monoclonal IgG1 IHC WB
Anti-HLA-DRA AMAb91674 Monoclonal IgG2a IHC WB
Anti-HLA-DRA AMAb91675 Monoclonal IgG2b IHC WB
Anti-IL17A AMAb91615 Monoclonal IgG1 IHC
Anti-IL17RA AMAb91617 Monoclonal IgG2b WB
Anti-IL17RA AMAb91619 Monoclonal IgG1 WB
Anti-IL7 AMAb91684 Monoclonal IgG1 ICC-IF
Anti-ITGA4 AMAb91699 Monoclonal IgG2b WB
Anti-MS4A1 AMAb91636 Monoclonal IgG2a IHC WB
Anti-OPALIN AMAb91685 Monoclonal IgG1 IHC
Anti-OPALIN AMAb91686 Monoclonal IgG2a IHC
Anti-PLP1 AMAb91639 Monoclonal IgG1 IHC
Anti-RGMA AMAb91702 Monoclonal IgG2b WB
Anti-S100A8 AMAb91689 Monoclonal IgG1 IHC
Anti-S100A9 AMAb91690 Monoclonal IgG2a IHC
Anti-SERPINA3 AMAb91655 Monoclonal IgG1 IHC
Anti-SPP1 AMAb91653 Monoclonal IgG1 IHC
Anti-TLR2 AMAb91631 Monoclonal IgG1 WB
Anti-TRPM4 AMAb91693 Monoclonal IgG1 IHC WB ICC-IF
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Anti-AGER HPA064436 Polyclonal WB ICC-IF 41 41
Anti-AIF1 HPA049234 Polyclonal IHC 84 84
Anti-AIF1 HPA062949 Polyclonal ICC-IF 86 83
Anti-ANO2 HPA057499 Polyclonal IHC 84 85
Anti-CD163 HPA046404 Polyclonal IHC ICC-IF 51 30
Anti-CD163 HPA051974 Polyclonal IHC 75 83
Anti-CRYAB HPA057100 Polyclonal IHC 98 98
Anti-DYSF HPA017071 Polyclonal IHC ICC-IF 96 96
Anti-DYSF HPA021945 Polyclonal IHC 88 88
Anti-GAP43 HPA013392 Polyclonal IHC ICC-IF 88 86
Anti-GAP43 HPA015600 Polyclonal IHC ICC-IF 71 70
Anti-GPR17 HPA029766 Polyclonal IHC 92 92
Anti-GZMB HPA003418 Polyclonal IHC WB 63 63
Anti-HLA-DRA HPA050162 Polyclonal IHC 54 69
Anti-HLA-DRA HPA053176 Polyclonal IHC 41 83
Anti-IL7 HPA019590 Polyclonal IHC 46 46
Anti-ITGA4 HPA074961 Polyclonal ICC-IF 90 89
Anti-MS4A1 HPA014341 Polyclonal IHC WB ICC-IF 73 77
Anti-MS4A1 HPA014391 Polyclonal IHC WB 61 64
Anti-OPALIN HPA014372 Polyclonal IHC 72 75
Anti-PLP1 HPA004128 Polyclonal IHC 100 100
Anti-RGMA HPA039880 Polyclonal ICC-IF 96 96
Anti-RGMA HPA044222 Polyclonal ICC-IF 87 87
Anti-S100A8 HPA024372 Polyclonal IHC WB ICC-IF 56 60
Anti-SERPINA3 HPA000893 Polyclonal IHC WB 61 61
Anti-SERPINA3 HPA002560 Polyclonal IHC WB 60 59
Anti-SPP1 HPA027541 Polyclonal IHC WB ICC-IF 56 53
Anti-TRPM4 HPA041169 Polyclonal IHC ICC-IF 93 91
NEW PRODUCTS
Selected Triple A PolyclonalsTM for Neuroinflammation
Products with enhanced validation for indicated application (see page 16)
Synaptic signaling is cell-cell communication that occurs at the synaptic level Cells communicate with each other via direct contact (juxtacrine signaling) over short distances (paracrine signaling) or vast distances and scales (endocrine signaling) Other cells form gap junctions that connect their cytoplasm to the cytoplasm of adjacent cells
When the sending neuron fires an electrical impulse travels along the axon As soon as the impulse reaches the synapse it triggers the release of neurotransmitters
The predominant excitatory neurotransmitter in the brain is glutamate (GLU) The inhibitory neurotransmitters GABA (in the brain) and glycine (in the brainstem and spinal cord) are common amino acids that participate in multiple metabolic cycles
Calcium (Ca2+) signaling in neurons is critical in cell signalingIt defines the ability of cells to release neurotransmitters thus regulating the intracellular signaling pathways Calbindin 2 (also known as calretinin) is one of the Ca2+-binding proteins involved in Ca2+ signaling that facilitates cytosolic Ca2+ diffusion without interfering with Ca2+ signaling
SYNAPTIC SIGNALING
A
nti-CALB2 antibody
An
ti-mGluR1 antibody
A
nti-VGAT antibody
Anti-VGAT monoclonal antibody (AMAb91043) shows strong immunoreactivity in GABAergic
fibers in the rat globus pallidus in green
Anti-CALB2 polyclonal antibody (HPA007305) shows the labeling in green of mouse
hippocampus Note the strong labeling in the dentate gyrus
Anti-MGLUR1 polyclonal antibody (HPA015701) strongly labels cortical perikarya in human
cerebral cortex tissue in brown
8
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Synaptic Signaling
9
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Anti-ATF2 HPA022134 Polyclonal IHC WB ICC-IF 99 99
Anti-ATF3 AMAb90909 Monoclonal IHC ICC-IF 92 92
Anti-ATF3 HPA001562 Polyclonal IHC ICC-IF 92 92
Anti-ATP1B1 HPA012911 Polyclonal IHC WB 93 93
Anti-ATP1B2 HPA010698 Polyclonal IHC 96 88
Anti-CAMK2B HPA026307 Polyclonal IHC WB 96 96
Anti-CALB1 HPA023099 Polyclonal IHC WB ICC-IF 98 99
Anti-CALB2 HPA007306 Polyclonal IHC WB ICC-IF 100 100
Anti-CALB2 HPA007305 Polyclonal IHC WB ICC-IF 100 100
Anti-CAMK2D HPA026281 Polyclonal IHC 100 97
Anti-C-FOS HPA018531 Polyclonal IHC ICC-IF 94 94
Anti-CHAT AMAb91130 Monoclonal IHC ICC-IF 96 96
Anti-CHAT HPA048547 Polyclonal IHC 96 96
Anti-CHRM1 (M1 mAChR) HPA014101 Polyclonal IHC 98 97
Anti-CHRM2 (M2 AChR) HPA029795 Polyclonal IHC 88 86
Anti-CLIC4 HPA008019 Polyclonal IHC WB 98 97
Anti-CREB1 HPA019150 Polyclonal IHC WB ICC-IF 100 100
Anti-DDC AMAb91089 Monoclonal IHCWB 90 88
Anti-DDC HPA017742 Polyclonal IHCWBICC-IF 90 88
Anti-EAAC1 HPA020086 Polyclonal IHC WB 77 79
Anti-EAAT2 HPA009172 Polyclonal IHC 87 89
Anti-GABRA3 HPA000839 Polyclonal IHCWB 91 93
Anti-GABRB1 HPA051297 Polyclonal IHC 97 100
Anti-GABRG1 HPA035622 Polyclonal IHC 96 94
Anti-GAD1 (GAD67) AMAb91076 Monoclonal IHCWB 94 94
Anti-GAD1 HPA058412 Polyclonal IHCWB 94 94
Anti-GAD2 AMAb91048 Monoclonal IHCWB 84 88
Anti-GAD2 HPA044637 Polyclonal IHC 84 88
Anti-GAT1 HPA013341 Polyclonal IHC WB 98 98
Anti-GAT3 HPA037981 Polyclonal IHCWB 85 87
Anti-GLUR2 (AMPA2) HPA008441 Polyclonal IHC 100 100
Anti-HTR2A HPA014011 Polyclonal IHC 95 97
Anti-KCC4 HPA041652 Polyclonal IHCWB 84 82
Anti-KCNJ5 (KIR34) HPA017353 Polyclonal IHCWB 89 89
Anti-KIF11 HPA010568 Polyclonal IHCWB 88 83
Anti-KIF17 HPA032085 Polyclonal IHCICC-IF 85 82
Anti-KIF18A HPA039312 Polyclonal IHCWBICC-IF 80 82
Anti-KIF1C HPA024602 Polyclonal IHCWB 81 83
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Anti-KIF26B HPA028562 Polyclonal IHCICC-IF 88 80
Anti-KIF4A (KIF4A amp B) HPA034745 Polyclonal IHCWBICC-IF 64 63
Anti-KIF5A HPA004469 Polyclonal IHCWB 91 88
Anti-KIF5C HPA035210 Polyclonal IHCWB 100100
Anti-KIF7 HPA043145 Polyclonal IHC 69 69
Anti-KIFAP3 HPA023742 Polyclonal IHC 100 100
Anti-MAPK1 (ERK) HPA030069 Polyclonal IHCWB 100 100
Anti-MAPK3 (ERK1) HPA005700 Polyclonal IHCWB 98 98
Anti-MGLUR1 HPA015701 Polyclonal IHC 80 80
Anti-MGLUR8 HPA051481 Polyclonal IHC 95 90
Anti-NCS1 HPA019713 Polyclonal IHCWBICC-IF 100 100
Anti-PRKCA HPA006563 Polyclonal IHCWBICC-IF 99 99
Anti-PRKCH HPA053709 Polyclonal IHCICC-IF 97 64
Anti-PNMT HPA051005 Polyclonal IHC 89 92
Anti-PRKACB (PKACB) HPA029754 Polyclonal IHC 73 76
Anti-PRKCZ HPA021851 Polyclonal IHCWB ICC-IF 94 94
Anti-RAB3A HPA003160 Polyclonal IHC 99 99
Anti-RAP1GAP HPA001922 Polyclonal IHC 92 91
Anti-SLC17A7 (VGLUT1) AMAb91041 Monoclonal IHCWB 94 94
Anti-SLC22A2 HPA008567 Polyclonal IHC 84 77
Anti-SLC32A1 (VGAT) HPA058859 Polyclonal IHC 95 93
Anti-SLC6A2 (NET) AMAb91116 Monoclonal IHC -
Anti-SLC6A3 (DAT) AMAb91125 Monoclonal IHC 85 85
Anti-SNAP25 HPA001830 Polyclonal IHCWB 100 100
Anti-SNAP29 HPA031823 Polyclonal IHC 89 92
Anti-SST (SOM) HPA019472 Polyclonal IHCWB 98 98
Anti-STXBP1 HPA008209 Polyclonal IHCWBICC-IF 100 100
Anti-STXBP6 HPA003552 Polyclonal IHCWB 99 99
Anti-SYNGR1 HPA029673 Polyclonal IHC ICC-IF 90 88
Anti-SYNPR HPA061671 Polyclonal IHC 97 97
Anti-SYP HPA002858 Polyclonal IHCWB 83 83
Anti-SYT1 HPA008394 Polyclonal IHCWB 100 100
Anti-SYT12 HPA011006 Polyclonal IHC 96 98
Anti-SYT13 HPA046224 Polyclonal IHC 96 93
Anti-SYT16 HPA004199 Polyclonal IHCWB 95 95
Anti-TGFA HPA042297 Polyclonal IHC 93 93
Anti-TH AMAb91112 Monoclonal IHC 88 88
Anti-TH HPA061003 Polyclonal IHC WB 88 88
Anti-TPH2 (NTPH) AMAb91108 Monoclonal IHC 100 100
Anti-VAMP4 HPA050418 Polyclonal IHCICC-IF 100 100
Anti-VAMP7 HPA036733 Polyclonal IHCICC-IF 98 93
Products with enhanced validation for indicated application (see page 16)
NEURAL LINEAGE
A
nti-GFAP antibody
Anti-C
NP antibody
Anti-N
EFM antibodyDuring central nervous system development neural stem cells (NSCs) proliferate and divide to generate a progeny that differentiate into neurons astrocytes oligodendrocytes and different glial cells
NSCs self-renewal and differentiation are regulated by a precise temporal sequence of growth factors intracellular signaling and transcription factor expression NSCs and their differentiated progeny can be identified by the expression of a unique combination of cell surface markers and transcription factors
The glial fibrillary acidic protein (GFAP) is an intermediate filament (IF) protein and a well-known marker for astrocytes
Oligodendrocytes can convert extracellular 2rsquo3rsquo-cAMP to adenosine As CNPase is the predominant enzyme involved in this conversion the cyclic nucleotide phosphodiesterase (CNP) is widely used as an oligodendrocyte marker
Neurons express the neurofilament medium (NFEM) polypeptide other intermediate filament proteins including nestin synemin syncoilin and vimentin
Anti-GFAP polyclonal antibody (HPA056030) shows strong immunoreactivity in the
human cerebellum in brown
Anti-NEFM polyclonal antibody (HPA022845) shows positivity in a subset of neuronal cells in
human cerebral cortex in brown
Anti-CNP polyclonal antibody (HPA023266) in the rat cerebral cortex is shown in magenta
10
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Neural Lineage
Products with enhanced validation for indicated application (see page 16)
11
TargetCells
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Astrocytes Anti-GFAP AMAb91033 Monoclonal IHC WB 98 100
Astrocytes Anti-GFAP HPA056030 Polyclonal IHCWB ICC-IF 98 100
Astrocytes Anti-GLUL AMAb91101 Monoclonal IHC WB 95 53
Astrocytes Anti-GLUL AMAb91102 Monoclonal IHC WB 95 92
Astrocytes Anti-GLUL AMAb91103 Monoclonal IHC WB 95 92
Astrocytes Anti-GLUL HPA007316 Polyclonal IHCWB 95 53
Astrocytes Anti-S100B AMAb91038 Monoclonal IHC WB 99 98
Astroglia Anti-EZR AMAb90976 Monoclonal IHCWBICC-IF 93 93
Astroglia Anti-EZR HPA021616 Polyclonal IHCWBICC-IF 93 93
Adhesion molecule Anti-ICAM5 HPA009083 Polyclonal IHC 85 86
Adhesion molecule Anti-MCAM HPA008848 Polyclonal IHC WB 75 73
Adhesion molecule Anti-NCAM2 HPA030900 Polyclonal IHC 89 91
Adhesion molecule Anti-NLGN1 HPA006680 Polyclonal IHCWB 98 98
Calcium binding protein Anti-CALB1 (CB) HPA023099 Polyclonal IHCWBICC-IF 98 99
Calcium binding protein Anti-CALB2 (CR) HPA007305 Polyclonal IHCWBICC-IF 100 100
Calcium binding proteins Anti-NECAB1 AMAb90801 Monoclonal IHCWB 98 98
Calcium binding protein Anti-NECAB1 HPA023629 Polyclonal IHCWB 98 98
Calcium binding protein Anti-NECAB2 AMAb90808 Monoclonal IHC 85 84
Calcium binding protein Anti-NECAB2 HPA013998 Polyclonal IHCICC-IF 98 97
Calcium binding protein Anti-SCGN AMAb90630 Monoclonal IHCWB 96 96
Calcium binding protein Anti-SCGN HPA006641 Polyclonal IHC 96 96
Calcium binding protein Anti-S100B HPA015768 Polyclonal IHCWBICC-IF 99 98
Oligodendrocytes Anti-MOG AMAb91066 Monoclonal IHC WB 91 89
Oligodendrocytes Anti-MOG AMAb91067 Monoclonal IHC WB 91 89
Oligodendrocytes Anti-MOG HPA021873 Polyclonal IHC 91 89
Oligodendrocytes Anti-CNP AMAb91068 Monoclonal IHC WB 76 77
Oligodendrocytes Anti-CNP AMAb91069 Monoclonal IHC WB 76 77
Oligodendrocytes Anti-CNP AMAb91072 Monoclonal IHC WB ICC-IF 76 77
Oligodendrocytes Anti-CNP HPA023280 Polyclonal IHCWBICC-IF 76 77
Progenitors Anti-PBK HPA005753 Polyclonal IHCWBICC-IF 91 94
Progenitors Anti-MKI67 (Ki67) AMAb90870 Monoclonal IHC ICC-IF 68 68
Progenitors Anti-MKI67 (Ki67) HPA000451 Polyclonal IHCICC-IF 66 67
TargetCells
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Cytoskeleton Anti-ACTN1 HPA006035 Polyclonal IHCWB 98 99
Cytoskeleton Anti-ACTN4 HPA001873 Polyclonal IHCWBICC-IF 99 98
Cytoskeleton Anti-INA HPA008057 Polyclonal IHC WB ICC-IF 83 84
Cytoskeleton Anti-MAP1A HPA039064 Polyclonal IHC 60 52
Cytoskeleton Anti-MAP1B HPA022275 Polyclonal IHCWB ICC-IF 85 86
Cytoskeleton Anti-MAP2 HPA008273 Polyclonal IHC WB ICC-IF 96 96
Cytoskeleton Anti-MYO5A HPA001356 Polyclonal IHCICC-IF 99 98
Cytoskeleton Anti-NEFM (NF160) HPA022845 Polyclonal IHCWBICC-IF 98 98
Microglia Anti-AIF HPA049234 Polyclonal IHC 84 84
Microglia Anti-CD68 AMAb90874 Monoclonal IHCWB 76 76
Microglia Anti-CD68 HPA048982 Polyclonal IHC WB 76 76
Microglia Anti-ITGAM (CD11b) AMAb90911 Monoclonal IHC WB 67 68
Microglia Anti-PTPRC AMAb90518 Monoclonal IHCWB 35 37
Microglia Anti-PTPRC HPA000440 Polyclonal IHCWB 35 37
Microglia Anti-TMEM119 AMAb91528 Monoclonal IHCWB ICC-IF 52 46
Microglia Anti-TMEM119 HPA051870 Polyclonal IHC 52 46
Neurons Anti-NEFM (NF160) AMAb91027 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91028 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91029 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91030 Monoclonal IHC WB 98 98
Neurons Anti-NEFH (NF200) AMAb91025 Monoclonal IHC WB 98 98
Neurons Anti-NEFH (NF200) HPA061615 Polyclonal IHC 88 94
Neurons Anti-UCHL1 AMAb91145 Monoclonal IHC WB ICC-IF 97 97
Neurons Anti-UCHL1 HPA005993 Polyclonal IHCWBICC-IF 97 97
Neurons Anti-VIM AMAb90516 Monoclonal IHCWB 99 99
Neurons Anti-VIM HPA001762 Polyclonal IHCWBICC-IF 99 99
Schwann cells Anti-MBP AMAb91062 Monoclonal IHC WB ICC-IF 97 97
Schwann cells Anti-MBP AMAb91063 Monoclonal IHC WB ICC-IF 97 97
Schwann cells Anti-MBP AMAb91064 Monoclonal IHC WB 97 97
Anti-I
TM2B antibodyNeurodegenerative diseases such as Amyotrophic Lateral Sclerosis Parkinsonrsquos Alzheimerrsquos and Huntingtonrsquos diseases occur as a result of the progressive loss of structure and function in neurons leading to the death of neurons
Age remains the primary risk factor for almost all the neurodegenerative disorders thus interfering with age-related molecular mechanisms or pathways might be an avenue to develop new therapeutics
The choline acetyltransferase (ChAT) is an enzyme responsible for catalyzing the synthesis of acetylcholine and is expressed in the majority of cholinergic neurons Hence ChAT immunoreactivity is used to detect cognitive decline in several neurodegenerative disorders
Integral membrane protein 2B (ITM2B) is a protein that in humans is encoded by the ITM2B gene This gene located on chromosome 13 is connected to familial dementia causing amyloid and pre-fibrillar effects similar to those seen in Alzheimerrsquos
The expression of the ubiquitin-carboxyl-terminal hydrolase isozyme L1 (UCHL1) present both in the neuronal cells of the central and peripheral nervous system is down-regulated in Parkinsonrsquos and Alzheimerrsquos disease patients
NEURODEGENERATIVE DISEASES
Anti-C
HAT antibody
Anti-U
CHL1 antibody
Anti-CHAT monoclonal antibody (AMAb91130) shows strong immunoreactivity in the
acetylcholine neurons in the rat diagonal band of Broca in green
Anti-ITM2B polyclonal antibody (HPA029292) targeting Integral membrane protein 2B strongly
labels Golgi apparatus in A-431 cells in green
Anti-UCHL1 monoclonal antibody (AMAb91145) in the mouse trigeminal ganglion show strong
immunoreactrivity in primary sensory neurons in green
12
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for NeurodegenerationProduct Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-ADAR AMAb90535 Monoclonal IHCWB ICC-IF 86 85
Anti-ADAR HPA003890 Polyclonal IHCWBICC-IF 86 85
Anti-AIMP1 HPA018476 Polyclonal IHCWBICC-IF 96 97
Anti-AKT1 AMAb90834 Monoclonal WB ICC-IF 97 97
Anti-AOX1 HPA040199 Polyclonal IHC 84 86
Anti-APBA3 HPA045577 Polyclonal IHC 70 68
Anti-APBB2 HPA023542 Polyclonal IHCWBICC-IF 85 83
Anti-APBB3 HPA005571 Polyclonal IHCICC-IF 84 84
Anti-APOA4 AMAb90769 Monoclonal IHC WB 65 28
Anti-APP HPA001462 Polyclonal IHCWB 95 95
Anti-AQP4 AMAb90537 Monoclonal IHCWB 93 92
Anti-AQP4 HPA014784 Polyclonal IHCWB ICC-IF 93 92
Anti-ATF2 HPA022134 Polyclonal IHCWBICC-IF 99 99
Anti-ATF3 AMAb90909 Monoclonal IHCICC-IF 92 92
Anti-ATF3 HPA001562 Polyclonal IHCICC-IF 92 92
Anti-ATRX AMAb90784 Monoclonal IHC WBICC-IF 96 97
Anti-ATXN1 HPA008335 Polyclonal IHC 81 81
Anti-ATXN2 HPA018295 Polyclonal IHCWBICC-IF 90 91
Anti-C3 HPA020432 Polyclonal IHC 78 23
Anti-CASP3 HPA002643 Polyclonal IHCWBICC-IF 84 88
Anti-CD4 AMAb90754 Monoclonal IHCWB 55 57
Anti-CD40 AMAb90905 Monoclonal IHCWB 58 54
Anti-CHAT AMAb91130 Monoclonal IHCICC-IF 96 96
Anti-CHAT HPA048547 Polyclonal IHC 96 96
Anti-CHGA AMAb90525 Monoclonal IHCWBICC-IF 62 64
Anti-COX1 HPA002834 Polyclonal IHCWBICC-IF 93 90
Anti-COX2PTGS2 HPA001335 Polyclonal IHCICC-IF 88 88
Anti-CTSB HPA018156 Polyclonal IHCICC-IF 79 79
Anti-CTSD HPA003001 Polyclonal IHCWB 86 86
Anti-CXorf27 HPA003356 Polyclonal IHC 47 46
Anti-FBXO7 (PARK15) HPA032114 Polyclonal IHC 78 81
Anti-FUS AMAb90549 Monoclonal IHC WB ICC-IF 91 91Anti-GSK3B HPA028017 Polyclonal IHCWBICC-IF 100 100Anti-HIP1 HPA017964 Polyclonal IHCWB 79 77Anti-HTRA2 (PARK13) HPA027366 Polyclonal IHCWB 57 63Anti-ITGAM (CD11b) AMAb90911 Monoclonal IHCWB 67 68
Anti-ITM2B HPA029292 Polyclonal IHC ICC-IF 95 96Anti-LRP2 HPA005980 Polyclonal IHC 78 36
Products with enhanced validation for indicated application (see page 16)
13
Product Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-NFKB1 HPA027305 Polyclonal IHCWBICC-IF 60 62
Anti-OPTN HPA003360 Polyclonal IHCWBICC-IF 64 68
Anti-PADI4 HPA017007 Polyclonal IHCWB 66 69
Anti-PARK7 HPA004190 Polyclonal IHCWB 89 90
Anti-PFN1 AMAb91181 Monoclonal IHCWB 87 87
Anti-PHGDH AMAb90786 Monoclonal IHCWBICC-IF 99 99
Anti-PHGDH HPA021241 Polyclonal IHCWBICC-IF 99 99
Anti-PRNP HPA042754 Polyclonal IHC 91 91
Anti-PSEN1 HPA030760 Polyclonal IHC 82 81
Anti-RHOT1 AMAb90852 Monoclonal IHCWB 100 100
Anti-RHOT1 HPA010687 Polyclonal IHC 100 100
Anti-S100A8 HPA024372 Polyclonal IHCWBICC-IF 56 60
Anti-SERPINA3 HPA002560 Polyclonal IHCWB 60 59
Anti-SNCB HPA035876 Polyclonal IHCWB 97 97
Anti-SOD1 HPA001401 Polyclonal IHCWBICC-IF 82 81
Anti-SOD2 HPA001814 Polyclonal IHCWB 88 87
Anti-THY1 AMAb90844 Monoclonal IHCWB 64 68
Anti-THY1 HPA003733 Polyclonal IHCICC-IF 64 68
Anti-TNFRSF21 HPA006746 Polyclonal IHCWB 86 85
Anti-TTR AMAb90649 Monoclonal IHC WB 81 83
Anti-UBE2K HPA028869 Polyclonal IHCICC-IF 100 100
Anti-UCHL1 HPA005993 Polyclonal IHCWBICC-IF 97 97
Anti-USP46 AMAb90722 Monoclonal WBICC-IF 100 99
Anti-VPS26A AMAb90967 Monoclonal IHC WBICC-IF 96 100
Anti-WHSC1 AMAb90851 Monoclonal IHCWBICC-IF 91 91
Anti-WHSC1 HPA015801 Polyclonal IHCICC-IF 91 91
Anti-VWF AMAb90928 Monoclonal IHCWB 80 80
Anti-VWF HPA002082 Polyclonal IHC 82 78
The development of the central nervous system includes the development of the brain spinal cord optic and auditory systems
Within the neural tube stem cells generate the two major classes of cells that make the majority of the nervous system neurons and glia Both these classes of cells differentiate into many different types generated with highly specialized functions and shapes
The development of the peripheral and central nervous systems begins early in embryogenesis and can be traced through the use of lineage antibodies specific to each stage of neuronal development
The (SOX) family of transcription factors are well-established regulators of cell fate decisions during development
Vimentin (VIM) is a type III intermediate filament protein expressed in mesenchymal cells It is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both healthy development and metastatic progression
The cell surface glycoprotein Thy1 (CD90) is expressed on many mammalian cell types including neurons and serves as a human mesenchymal stem cell (MSC) marker
DEVELOPMENT
A
nti-VIM antibody
A
nti-SOX4 antibody
Anti-T
HY1 antibody
Anti-VIM polyclonal antibody (HPA001762) immunofluorescent staining of human cell line
ASC TERT1 shows localization to intermediate filaments in green
Anti-SOX4 monoclonal antibody (AMAb913802) immunofluorescent staining in mouse
hippocampus showing immunoreactivity in a subset of neurons in the granule cell layer of
the dentate gyrus in brown
Anti-THY1 polyclonal antibody (HPA003733) staining of human cerebral cortex shows
localization to neuropils in brown
Product Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-ENG AMAb90925 Monoclonal IHC 66 22Anti-ENG HPA011862 Polyclonal IHCWB 66 22Anti-FABP7 AMAb90595 Monoclonal IHCWB 89 90Anti-FABP7 HPA028825 Polyclonal IHCWB 89 90Anti-FLT1 AMAb90704 Monoclonal IHCWB 80 82Anti-GAP43 HPA015600 Polyclonal IHC ICC-IF 71 70Anti-GLI3 HPA005534 Polyclonal IHC 74 76Anti-MEF2C AMAb90727 Monoclonal IHCWBICC-IF 97 47Anti-MEF2C HPA005533 Polyclonal IHCWBICC-IF 97 47Anti-MKI67 (Ki67) HPA000451 Polyclonal IHCICC-IF 66 67Anti-NACC1 HPA021238 Polyclonal IHCICC-IF 91 89Anti-NES (Nestin) AMAb90556 Monoclonal IHCWBICC-IF 47 42Anti-NES (Nestin) HPA007007 Polyclonal IHCWB 47 42Anti-NKX2-2 HPA003468 Polyclonal IHCWB 96 97Anti-PAX6 HPA030775 Polyclonal IHCICC-IF 100 100Anti-PBK HPA005753 Polyclonal IHCWBICC-IF 91 94Anti-REST AMAb90740 Monoclonal IHCICC-IF 41 43Anti-RUNX1 HPA004176 Polyclonal IHCICC-IF 93 93Anti-RUNX2 AMAb90591 Monoclonal IHCWBICC-IF 100 81Anti-RUNX2 HPA022040 Polyclonal IHCWBICC-IF 100 81Anti-SATB2 AMAb90679 Monoclonal IHCWBICC-IF 100 100Anti-SATB2 HPA029543 Polyclonal IHCWBICC-IF 100 100Anti-SOX4 HPA029901 Polyclonal IHCICC-IF 100 39Anti-SOX6 HPA001923 Polyclonal IHCICC-IF 96 96Anti-SOX7 HPA009065 Polyclonal IHCWB 91 91Anti-SOX9 AMAb90795 Monoclonal IHCWBICC-IF 97 96Anti-SOX11 AMAb90502 Monoclonal IHCWB 82 82Anti-SOX11 HPA000536 Polyclonal IHCWB 82 82Anti-SOX30 HPA006159 Polyclonal IHCWB 68 70Anti-THY1 AMAb90844 Monoclonal IHCWB 64 68Anti-THY1 HPA003733 Polyclonal IHCICC-IF 64 68Anti-TM4SF2TSPAN7 HPA003140 Polyclonal IHCWB 96 96Anti-TM4SF2TSPAN7 AMAb90621 Monoclonal IHCWB 96 96Anti-VANGL1 AMAb90600 Monoclonal WBICC-IF 95 95Anti-VIM AMAb90516 Monoclonal IHCWB 99 99Anti-VIM HPA001762 Polyclonal IHCWBICC-IF 99 99
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Development
Products with enhanced validation for indicated application (see page 16)
15
At Atlas Antibodies we extensively validate our antibodies in IHC WB and ICC-IF Enhanced Validation is performed as an additional layer of security in an application and context-specific manner Enhanced validation offers increased security of antibody specificity in a defined context This is ensured by using the most relevant validation method for each combination of protein sample and application Enhanced Validation follows the guidelines proposed by the International Working Group for Antibody Validation (IWGAV)
ENHANCED VALIDATION
GENETIC VALIDATION Antibody specificity is confirmed by downregulating the target protein on a genetic level using siRNA
ORTHOGONAL VALIDATIONThe antibody is validated by comparing the results with a non-antibody based method across multiple samples
VALIDATION BY INDEPENDENT ANTIBODIESThe antibody specificity is demonstrated by comparing two antibodies targeting different regions of the same protein
RECOMBINANT EXPRESSION VALIDATIONThe antibody binding is verified using and over-expressed or tagged version of the target protein
MIGRATION CAPTURE MS VALIDATIONThe staining pattern and the protein size detected by the antibody is compared with results obtained by a capture Mass Spectrometry (MS) method
16
Triple A PolyclonalsTM are rabbit polyclonal primary antibodies developed within the Human Protein Atlas project IHC characterization data from 44 normal and 20 cancer tissues is available on the Human Protein Atlas portal
PrecisA MonoclonalsTM are mouse monoclonal primary antibodies developed against a number of carefully selected targets Clones are selected to recognize only unique non-overlapping epitopes and isotypes
Atlas Antibodies ABVoltavaumlgen 13SE-168 69 BrommaSwedenatlasantibodiescom
Phone +46(0)8 54 59 58 50contactatlasantibodiescomorderatlasantibodiescomsupportatlasantibodiescom
Atlas Antibodies logo Triple A Polyclonals PrecisA Monoclonals PrEST Antigen and QPrEST are trademarks or registered trademarks of Atlas Antibodies AB All other trademarks are the property of their respective owners
Products are for research use only Not for use in diagnostic procedures copy Atlas Antibodies AB 2020 008_
Neu
ro M
arke
r Pan
el _
Rev
03_2
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5_S
fN E
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Antibodies are valuable tools in the ongoing scientific effort to gain more knowledge about the human brain to understand the fundamental and emergent properties of neurons and neural circuits and to identify diseases such as neurological brain disorders Therefore there is a necessary need for highly-characterized and specific antibodies for neuroscience research
Our neuroscience catalog features a wide range of primary monoclonal and polyclonal antibodies for use in research designed to recognize the diverse neurochemical cell types in rodent and human nervous systems validated in cell lines rodents and human tissues
Our antibodies are validated for use in IHC WB and ICC-IF
ANTIBODIES FOR NEUROSCIENCE
ImmunofluorescenceWestern blot
Im
munohistochemistry3
VERY RELIABLE ANTIBODIESAtlas Antibodies is the original manufacturer of over 21000 primary antibodies targeting the majority of human proteins Our Triple A PolyclonalsTM and PrecisA MonoclonalsTM antibodies are available worldwide Building on our heritage with the Human Protein Atlas project we provide highly validated reagents that enable leading research in biology diagnostics and medicine for the purpose of understanding and improving human health
CREATED BY THE HUMAN PROTEIN ATLASTriple A Polyclonals are developed within the prestigious Human Protein Atlas project Our antibodies are validated in all major human tissues and organs and 20 cancer tissues Each antibody is supported by over 500 staining images
VALIDATED BY ENHANCED VALIDATIONWe take great care to validate our antibodies in IHC WB and ICC-IF As an additional layer of security we perform Enhanced Validation By using 5 different enhanced validation methods we validate our antibodies for each combination of protein sample and application
EVIDENCED BY SCIENCEOur antibodies are used all over the world and referenced in 1000s of scientific peer-reviewed papers
4
WHY ATLAS ANTIBODIES
The Human Protein Atlas
Atlas Antibodies
Learn more
NEUROINFLAMMATIONNeuroinflammation broadly defines the collective reactive immune response in the brain and spinal cord in response to injury and disease Inflammation in the central nervous system (CNS) is commonly associated with various degrees of tissue damage such as loss of myelin and neurons
The neuroinflammatory process is complex and involves disruption of the blood-brain barrier peripheral leukocyte infiltration edema and gliosis The inflammatory response is characterized by a host of cellular and molecular aberrations within the brain
Neuroinflammation arises locally within the CNS through phenotypic changes of different non-neuronal cell types in the brain such as microglia oligodendrocytes and astrocytes causing the release of different cytokines and chemokines and finally recruitment and infiltration of peripheral blood cells mainly T-and B-cells into the brain parenchyma
Neuroinflammatory processes are the key causative factors behind the brain and spinal cord injury This is true not only for acute brain trauma and hypoxic-ischemic brain damage following stroke but also for chronic infection and neurodegenerative diseases such as Alzheimerrsquos disease (AD) amyotrophic lateral sclerosis (ALS) Lewy body dementia (LBD) and multiple sclerosis (MS) In addition local peritumoral inflammation plays a role in the clinical progression and malignancy of glioblastomas the most aggressive primary brain tumors
Ant
i-GFA
P Anti-MBP Anti-P2RY12
Anti-
HLA-DRA antibody
Anti-
HLA-DRA Anti-GFAP
Anti-HLA-DRA (AMAb91674) shows microglial cells in red Anti-GFAP (AMAb91033) shows
astrocytes in green in Alzheimerrsquos disease cerebral cortex
Anti-P2RY12 (HPA014518) shows microglial cells in green Anti-GFAP(AMAb91033) shows
astrocytes in blue Anti-MBP (AMAb91062) shows myelinated processes in red in
Alzheimerrsquos disease cerebral cortex
Anti-HLA-DRA monoclonal antibody (AMAb91674) shows strong positivity in
microglial cells in Alzheimerrsquos disease cerebral cortex
6
New PrecisA MonoclonalsTM for Neuroinflammation
Products with enhanced validation for indicated application (see page 16)
7
Product Name
Product Number Clonality Validated
ApplicationsAnti-AGER AMAb91634 Monoclonal IgG2a IHC
Anti-AGER AMAb91635 Monoclonal IgG1 IHC
Anti-AIF1 AMAb91671 Monoclonal IgG2b IHC
Anti-AIF1 AMAb91672 Monoclonal IgG1 IHC
Anti-AMIGO3 AMAb91677 Monoclonal IgG1 WB
Anti-ANO2 AMAb91641 Monoclonal IgG1 IHC
Anti-ANO2 AMAb91642 Monoclonal IgG1 IHC
Anti-CD163 AMAb91646 Monoclonal IgG1 IHCWB
Anti-CD163 AMAb91648 Monoclonal IgG1 IHC
Anti-CRYAB AMAb91661 Monoclonal IgG1 IHC WB ICC-IF
Anti-CRYAB AMAb91662 Monoclonal IgG2b IHC WB
Anti-CXCL13 AMAb91629 Monoclonal IgG1 IHC
Anti-DYSF AMAb91667 Monoclonal IgG1 IHC WB
Anti-GAP43 AMAb91664 Monoclonal IgG2b IHC
Anti-GAP43 AMAb91665 Monoclonal IgG1 IHC
Anti-GAP43 AMAb91666 Monoclonal IgG2a IHC
Anti-GPR17 AMAb91624 Monoclonal IgG1 IHC WB
Anti-GZMB AMAb91650 Monoclonal IgG1 IHC
Anti-HLA-DRA AMAb91673 Monoclonal IgG1 IHC WB
Anti-HLA-DRA AMAb91674 Monoclonal IgG2a IHC WB
Anti-HLA-DRA AMAb91675 Monoclonal IgG2b IHC WB
Anti-IL17A AMAb91615 Monoclonal IgG1 IHC
Anti-IL17RA AMAb91617 Monoclonal IgG2b WB
Anti-IL17RA AMAb91619 Monoclonal IgG1 WB
Anti-IL7 AMAb91684 Monoclonal IgG1 ICC-IF
Anti-ITGA4 AMAb91699 Monoclonal IgG2b WB
Anti-MS4A1 AMAb91636 Monoclonal IgG2a IHC WB
Anti-OPALIN AMAb91685 Monoclonal IgG1 IHC
Anti-OPALIN AMAb91686 Monoclonal IgG2a IHC
Anti-PLP1 AMAb91639 Monoclonal IgG1 IHC
Anti-RGMA AMAb91702 Monoclonal IgG2b WB
Anti-S100A8 AMAb91689 Monoclonal IgG1 IHC
Anti-S100A9 AMAb91690 Monoclonal IgG2a IHC
Anti-SERPINA3 AMAb91655 Monoclonal IgG1 IHC
Anti-SPP1 AMAb91653 Monoclonal IgG1 IHC
Anti-TLR2 AMAb91631 Monoclonal IgG1 WB
Anti-TRPM4 AMAb91693 Monoclonal IgG1 IHC WB ICC-IF
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Anti-AGER HPA064436 Polyclonal WB ICC-IF 41 41
Anti-AIF1 HPA049234 Polyclonal IHC 84 84
Anti-AIF1 HPA062949 Polyclonal ICC-IF 86 83
Anti-ANO2 HPA057499 Polyclonal IHC 84 85
Anti-CD163 HPA046404 Polyclonal IHC ICC-IF 51 30
Anti-CD163 HPA051974 Polyclonal IHC 75 83
Anti-CRYAB HPA057100 Polyclonal IHC 98 98
Anti-DYSF HPA017071 Polyclonal IHC ICC-IF 96 96
Anti-DYSF HPA021945 Polyclonal IHC 88 88
Anti-GAP43 HPA013392 Polyclonal IHC ICC-IF 88 86
Anti-GAP43 HPA015600 Polyclonal IHC ICC-IF 71 70
Anti-GPR17 HPA029766 Polyclonal IHC 92 92
Anti-GZMB HPA003418 Polyclonal IHC WB 63 63
Anti-HLA-DRA HPA050162 Polyclonal IHC 54 69
Anti-HLA-DRA HPA053176 Polyclonal IHC 41 83
Anti-IL7 HPA019590 Polyclonal IHC 46 46
Anti-ITGA4 HPA074961 Polyclonal ICC-IF 90 89
Anti-MS4A1 HPA014341 Polyclonal IHC WB ICC-IF 73 77
Anti-MS4A1 HPA014391 Polyclonal IHC WB 61 64
Anti-OPALIN HPA014372 Polyclonal IHC 72 75
Anti-PLP1 HPA004128 Polyclonal IHC 100 100
Anti-RGMA HPA039880 Polyclonal ICC-IF 96 96
Anti-RGMA HPA044222 Polyclonal ICC-IF 87 87
Anti-S100A8 HPA024372 Polyclonal IHC WB ICC-IF 56 60
Anti-SERPINA3 HPA000893 Polyclonal IHC WB 61 61
Anti-SERPINA3 HPA002560 Polyclonal IHC WB 60 59
Anti-SPP1 HPA027541 Polyclonal IHC WB ICC-IF 56 53
Anti-TRPM4 HPA041169 Polyclonal IHC ICC-IF 93 91
NEW PRODUCTS
Selected Triple A PolyclonalsTM for Neuroinflammation
Products with enhanced validation for indicated application (see page 16)
Synaptic signaling is cell-cell communication that occurs at the synaptic level Cells communicate with each other via direct contact (juxtacrine signaling) over short distances (paracrine signaling) or vast distances and scales (endocrine signaling) Other cells form gap junctions that connect their cytoplasm to the cytoplasm of adjacent cells
When the sending neuron fires an electrical impulse travels along the axon As soon as the impulse reaches the synapse it triggers the release of neurotransmitters
The predominant excitatory neurotransmitter in the brain is glutamate (GLU) The inhibitory neurotransmitters GABA (in the brain) and glycine (in the brainstem and spinal cord) are common amino acids that participate in multiple metabolic cycles
Calcium (Ca2+) signaling in neurons is critical in cell signalingIt defines the ability of cells to release neurotransmitters thus regulating the intracellular signaling pathways Calbindin 2 (also known as calretinin) is one of the Ca2+-binding proteins involved in Ca2+ signaling that facilitates cytosolic Ca2+ diffusion without interfering with Ca2+ signaling
SYNAPTIC SIGNALING
A
nti-CALB2 antibody
An
ti-mGluR1 antibody
A
nti-VGAT antibody
Anti-VGAT monoclonal antibody (AMAb91043) shows strong immunoreactivity in GABAergic
fibers in the rat globus pallidus in green
Anti-CALB2 polyclonal antibody (HPA007305) shows the labeling in green of mouse
hippocampus Note the strong labeling in the dentate gyrus
Anti-MGLUR1 polyclonal antibody (HPA015701) strongly labels cortical perikarya in human
cerebral cortex tissue in brown
8
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Synaptic Signaling
9
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Anti-ATF2 HPA022134 Polyclonal IHC WB ICC-IF 99 99
Anti-ATF3 AMAb90909 Monoclonal IHC ICC-IF 92 92
Anti-ATF3 HPA001562 Polyclonal IHC ICC-IF 92 92
Anti-ATP1B1 HPA012911 Polyclonal IHC WB 93 93
Anti-ATP1B2 HPA010698 Polyclonal IHC 96 88
Anti-CAMK2B HPA026307 Polyclonal IHC WB 96 96
Anti-CALB1 HPA023099 Polyclonal IHC WB ICC-IF 98 99
Anti-CALB2 HPA007306 Polyclonal IHC WB ICC-IF 100 100
Anti-CALB2 HPA007305 Polyclonal IHC WB ICC-IF 100 100
Anti-CAMK2D HPA026281 Polyclonal IHC 100 97
Anti-C-FOS HPA018531 Polyclonal IHC ICC-IF 94 94
Anti-CHAT AMAb91130 Monoclonal IHC ICC-IF 96 96
Anti-CHAT HPA048547 Polyclonal IHC 96 96
Anti-CHRM1 (M1 mAChR) HPA014101 Polyclonal IHC 98 97
Anti-CHRM2 (M2 AChR) HPA029795 Polyclonal IHC 88 86
Anti-CLIC4 HPA008019 Polyclonal IHC WB 98 97
Anti-CREB1 HPA019150 Polyclonal IHC WB ICC-IF 100 100
Anti-DDC AMAb91089 Monoclonal IHCWB 90 88
Anti-DDC HPA017742 Polyclonal IHCWBICC-IF 90 88
Anti-EAAC1 HPA020086 Polyclonal IHC WB 77 79
Anti-EAAT2 HPA009172 Polyclonal IHC 87 89
Anti-GABRA3 HPA000839 Polyclonal IHCWB 91 93
Anti-GABRB1 HPA051297 Polyclonal IHC 97 100
Anti-GABRG1 HPA035622 Polyclonal IHC 96 94
Anti-GAD1 (GAD67) AMAb91076 Monoclonal IHCWB 94 94
Anti-GAD1 HPA058412 Polyclonal IHCWB 94 94
Anti-GAD2 AMAb91048 Monoclonal IHCWB 84 88
Anti-GAD2 HPA044637 Polyclonal IHC 84 88
Anti-GAT1 HPA013341 Polyclonal IHC WB 98 98
Anti-GAT3 HPA037981 Polyclonal IHCWB 85 87
Anti-GLUR2 (AMPA2) HPA008441 Polyclonal IHC 100 100
Anti-HTR2A HPA014011 Polyclonal IHC 95 97
Anti-KCC4 HPA041652 Polyclonal IHCWB 84 82
Anti-KCNJ5 (KIR34) HPA017353 Polyclonal IHCWB 89 89
Anti-KIF11 HPA010568 Polyclonal IHCWB 88 83
Anti-KIF17 HPA032085 Polyclonal IHCICC-IF 85 82
Anti-KIF18A HPA039312 Polyclonal IHCWBICC-IF 80 82
Anti-KIF1C HPA024602 Polyclonal IHCWB 81 83
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Anti-KIF26B HPA028562 Polyclonal IHCICC-IF 88 80
Anti-KIF4A (KIF4A amp B) HPA034745 Polyclonal IHCWBICC-IF 64 63
Anti-KIF5A HPA004469 Polyclonal IHCWB 91 88
Anti-KIF5C HPA035210 Polyclonal IHCWB 100100
Anti-KIF7 HPA043145 Polyclonal IHC 69 69
Anti-KIFAP3 HPA023742 Polyclonal IHC 100 100
Anti-MAPK1 (ERK) HPA030069 Polyclonal IHCWB 100 100
Anti-MAPK3 (ERK1) HPA005700 Polyclonal IHCWB 98 98
Anti-MGLUR1 HPA015701 Polyclonal IHC 80 80
Anti-MGLUR8 HPA051481 Polyclonal IHC 95 90
Anti-NCS1 HPA019713 Polyclonal IHCWBICC-IF 100 100
Anti-PRKCA HPA006563 Polyclonal IHCWBICC-IF 99 99
Anti-PRKCH HPA053709 Polyclonal IHCICC-IF 97 64
Anti-PNMT HPA051005 Polyclonal IHC 89 92
Anti-PRKACB (PKACB) HPA029754 Polyclonal IHC 73 76
Anti-PRKCZ HPA021851 Polyclonal IHCWB ICC-IF 94 94
Anti-RAB3A HPA003160 Polyclonal IHC 99 99
Anti-RAP1GAP HPA001922 Polyclonal IHC 92 91
Anti-SLC17A7 (VGLUT1) AMAb91041 Monoclonal IHCWB 94 94
Anti-SLC22A2 HPA008567 Polyclonal IHC 84 77
Anti-SLC32A1 (VGAT) HPA058859 Polyclonal IHC 95 93
Anti-SLC6A2 (NET) AMAb91116 Monoclonal IHC -
Anti-SLC6A3 (DAT) AMAb91125 Monoclonal IHC 85 85
Anti-SNAP25 HPA001830 Polyclonal IHCWB 100 100
Anti-SNAP29 HPA031823 Polyclonal IHC 89 92
Anti-SST (SOM) HPA019472 Polyclonal IHCWB 98 98
Anti-STXBP1 HPA008209 Polyclonal IHCWBICC-IF 100 100
Anti-STXBP6 HPA003552 Polyclonal IHCWB 99 99
Anti-SYNGR1 HPA029673 Polyclonal IHC ICC-IF 90 88
Anti-SYNPR HPA061671 Polyclonal IHC 97 97
Anti-SYP HPA002858 Polyclonal IHCWB 83 83
Anti-SYT1 HPA008394 Polyclonal IHCWB 100 100
Anti-SYT12 HPA011006 Polyclonal IHC 96 98
Anti-SYT13 HPA046224 Polyclonal IHC 96 93
Anti-SYT16 HPA004199 Polyclonal IHCWB 95 95
Anti-TGFA HPA042297 Polyclonal IHC 93 93
Anti-TH AMAb91112 Monoclonal IHC 88 88
Anti-TH HPA061003 Polyclonal IHC WB 88 88
Anti-TPH2 (NTPH) AMAb91108 Monoclonal IHC 100 100
Anti-VAMP4 HPA050418 Polyclonal IHCICC-IF 100 100
Anti-VAMP7 HPA036733 Polyclonal IHCICC-IF 98 93
Products with enhanced validation for indicated application (see page 16)
NEURAL LINEAGE
A
nti-GFAP antibody
Anti-C
NP antibody
Anti-N
EFM antibodyDuring central nervous system development neural stem cells (NSCs) proliferate and divide to generate a progeny that differentiate into neurons astrocytes oligodendrocytes and different glial cells
NSCs self-renewal and differentiation are regulated by a precise temporal sequence of growth factors intracellular signaling and transcription factor expression NSCs and their differentiated progeny can be identified by the expression of a unique combination of cell surface markers and transcription factors
The glial fibrillary acidic protein (GFAP) is an intermediate filament (IF) protein and a well-known marker for astrocytes
Oligodendrocytes can convert extracellular 2rsquo3rsquo-cAMP to adenosine As CNPase is the predominant enzyme involved in this conversion the cyclic nucleotide phosphodiesterase (CNP) is widely used as an oligodendrocyte marker
Neurons express the neurofilament medium (NFEM) polypeptide other intermediate filament proteins including nestin synemin syncoilin and vimentin
Anti-GFAP polyclonal antibody (HPA056030) shows strong immunoreactivity in the
human cerebellum in brown
Anti-NEFM polyclonal antibody (HPA022845) shows positivity in a subset of neuronal cells in
human cerebral cortex in brown
Anti-CNP polyclonal antibody (HPA023266) in the rat cerebral cortex is shown in magenta
10
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Neural Lineage
Products with enhanced validation for indicated application (see page 16)
11
TargetCells
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Astrocytes Anti-GFAP AMAb91033 Monoclonal IHC WB 98 100
Astrocytes Anti-GFAP HPA056030 Polyclonal IHCWB ICC-IF 98 100
Astrocytes Anti-GLUL AMAb91101 Monoclonal IHC WB 95 53
Astrocytes Anti-GLUL AMAb91102 Monoclonal IHC WB 95 92
Astrocytes Anti-GLUL AMAb91103 Monoclonal IHC WB 95 92
Astrocytes Anti-GLUL HPA007316 Polyclonal IHCWB 95 53
Astrocytes Anti-S100B AMAb91038 Monoclonal IHC WB 99 98
Astroglia Anti-EZR AMAb90976 Monoclonal IHCWBICC-IF 93 93
Astroglia Anti-EZR HPA021616 Polyclonal IHCWBICC-IF 93 93
Adhesion molecule Anti-ICAM5 HPA009083 Polyclonal IHC 85 86
Adhesion molecule Anti-MCAM HPA008848 Polyclonal IHC WB 75 73
Adhesion molecule Anti-NCAM2 HPA030900 Polyclonal IHC 89 91
Adhesion molecule Anti-NLGN1 HPA006680 Polyclonal IHCWB 98 98
Calcium binding protein Anti-CALB1 (CB) HPA023099 Polyclonal IHCWBICC-IF 98 99
Calcium binding protein Anti-CALB2 (CR) HPA007305 Polyclonal IHCWBICC-IF 100 100
Calcium binding proteins Anti-NECAB1 AMAb90801 Monoclonal IHCWB 98 98
Calcium binding protein Anti-NECAB1 HPA023629 Polyclonal IHCWB 98 98
Calcium binding protein Anti-NECAB2 AMAb90808 Monoclonal IHC 85 84
Calcium binding protein Anti-NECAB2 HPA013998 Polyclonal IHCICC-IF 98 97
Calcium binding protein Anti-SCGN AMAb90630 Monoclonal IHCWB 96 96
Calcium binding protein Anti-SCGN HPA006641 Polyclonal IHC 96 96
Calcium binding protein Anti-S100B HPA015768 Polyclonal IHCWBICC-IF 99 98
Oligodendrocytes Anti-MOG AMAb91066 Monoclonal IHC WB 91 89
Oligodendrocytes Anti-MOG AMAb91067 Monoclonal IHC WB 91 89
Oligodendrocytes Anti-MOG HPA021873 Polyclonal IHC 91 89
Oligodendrocytes Anti-CNP AMAb91068 Monoclonal IHC WB 76 77
Oligodendrocytes Anti-CNP AMAb91069 Monoclonal IHC WB 76 77
Oligodendrocytes Anti-CNP AMAb91072 Monoclonal IHC WB ICC-IF 76 77
Oligodendrocytes Anti-CNP HPA023280 Polyclonal IHCWBICC-IF 76 77
Progenitors Anti-PBK HPA005753 Polyclonal IHCWBICC-IF 91 94
Progenitors Anti-MKI67 (Ki67) AMAb90870 Monoclonal IHC ICC-IF 68 68
Progenitors Anti-MKI67 (Ki67) HPA000451 Polyclonal IHCICC-IF 66 67
TargetCells
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Cytoskeleton Anti-ACTN1 HPA006035 Polyclonal IHCWB 98 99
Cytoskeleton Anti-ACTN4 HPA001873 Polyclonal IHCWBICC-IF 99 98
Cytoskeleton Anti-INA HPA008057 Polyclonal IHC WB ICC-IF 83 84
Cytoskeleton Anti-MAP1A HPA039064 Polyclonal IHC 60 52
Cytoskeleton Anti-MAP1B HPA022275 Polyclonal IHCWB ICC-IF 85 86
Cytoskeleton Anti-MAP2 HPA008273 Polyclonal IHC WB ICC-IF 96 96
Cytoskeleton Anti-MYO5A HPA001356 Polyclonal IHCICC-IF 99 98
Cytoskeleton Anti-NEFM (NF160) HPA022845 Polyclonal IHCWBICC-IF 98 98
Microglia Anti-AIF HPA049234 Polyclonal IHC 84 84
Microglia Anti-CD68 AMAb90874 Monoclonal IHCWB 76 76
Microglia Anti-CD68 HPA048982 Polyclonal IHC WB 76 76
Microglia Anti-ITGAM (CD11b) AMAb90911 Monoclonal IHC WB 67 68
Microglia Anti-PTPRC AMAb90518 Monoclonal IHCWB 35 37
Microglia Anti-PTPRC HPA000440 Polyclonal IHCWB 35 37
Microglia Anti-TMEM119 AMAb91528 Monoclonal IHCWB ICC-IF 52 46
Microglia Anti-TMEM119 HPA051870 Polyclonal IHC 52 46
Neurons Anti-NEFM (NF160) AMAb91027 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91028 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91029 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91030 Monoclonal IHC WB 98 98
Neurons Anti-NEFH (NF200) AMAb91025 Monoclonal IHC WB 98 98
Neurons Anti-NEFH (NF200) HPA061615 Polyclonal IHC 88 94
Neurons Anti-UCHL1 AMAb91145 Monoclonal IHC WB ICC-IF 97 97
Neurons Anti-UCHL1 HPA005993 Polyclonal IHCWBICC-IF 97 97
Neurons Anti-VIM AMAb90516 Monoclonal IHCWB 99 99
Neurons Anti-VIM HPA001762 Polyclonal IHCWBICC-IF 99 99
Schwann cells Anti-MBP AMAb91062 Monoclonal IHC WB ICC-IF 97 97
Schwann cells Anti-MBP AMAb91063 Monoclonal IHC WB ICC-IF 97 97
Schwann cells Anti-MBP AMAb91064 Monoclonal IHC WB 97 97
Anti-I
TM2B antibodyNeurodegenerative diseases such as Amyotrophic Lateral Sclerosis Parkinsonrsquos Alzheimerrsquos and Huntingtonrsquos diseases occur as a result of the progressive loss of structure and function in neurons leading to the death of neurons
Age remains the primary risk factor for almost all the neurodegenerative disorders thus interfering with age-related molecular mechanisms or pathways might be an avenue to develop new therapeutics
The choline acetyltransferase (ChAT) is an enzyme responsible for catalyzing the synthesis of acetylcholine and is expressed in the majority of cholinergic neurons Hence ChAT immunoreactivity is used to detect cognitive decline in several neurodegenerative disorders
Integral membrane protein 2B (ITM2B) is a protein that in humans is encoded by the ITM2B gene This gene located on chromosome 13 is connected to familial dementia causing amyloid and pre-fibrillar effects similar to those seen in Alzheimerrsquos
The expression of the ubiquitin-carboxyl-terminal hydrolase isozyme L1 (UCHL1) present both in the neuronal cells of the central and peripheral nervous system is down-regulated in Parkinsonrsquos and Alzheimerrsquos disease patients
NEURODEGENERATIVE DISEASES
Anti-C
HAT antibody
Anti-U
CHL1 antibody
Anti-CHAT monoclonal antibody (AMAb91130) shows strong immunoreactivity in the
acetylcholine neurons in the rat diagonal band of Broca in green
Anti-ITM2B polyclonal antibody (HPA029292) targeting Integral membrane protein 2B strongly
labels Golgi apparatus in A-431 cells in green
Anti-UCHL1 monoclonal antibody (AMAb91145) in the mouse trigeminal ganglion show strong
immunoreactrivity in primary sensory neurons in green
12
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for NeurodegenerationProduct Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-ADAR AMAb90535 Monoclonal IHCWB ICC-IF 86 85
Anti-ADAR HPA003890 Polyclonal IHCWBICC-IF 86 85
Anti-AIMP1 HPA018476 Polyclonal IHCWBICC-IF 96 97
Anti-AKT1 AMAb90834 Monoclonal WB ICC-IF 97 97
Anti-AOX1 HPA040199 Polyclonal IHC 84 86
Anti-APBA3 HPA045577 Polyclonal IHC 70 68
Anti-APBB2 HPA023542 Polyclonal IHCWBICC-IF 85 83
Anti-APBB3 HPA005571 Polyclonal IHCICC-IF 84 84
Anti-APOA4 AMAb90769 Monoclonal IHC WB 65 28
Anti-APP HPA001462 Polyclonal IHCWB 95 95
Anti-AQP4 AMAb90537 Monoclonal IHCWB 93 92
Anti-AQP4 HPA014784 Polyclonal IHCWB ICC-IF 93 92
Anti-ATF2 HPA022134 Polyclonal IHCWBICC-IF 99 99
Anti-ATF3 AMAb90909 Monoclonal IHCICC-IF 92 92
Anti-ATF3 HPA001562 Polyclonal IHCICC-IF 92 92
Anti-ATRX AMAb90784 Monoclonal IHC WBICC-IF 96 97
Anti-ATXN1 HPA008335 Polyclonal IHC 81 81
Anti-ATXN2 HPA018295 Polyclonal IHCWBICC-IF 90 91
Anti-C3 HPA020432 Polyclonal IHC 78 23
Anti-CASP3 HPA002643 Polyclonal IHCWBICC-IF 84 88
Anti-CD4 AMAb90754 Monoclonal IHCWB 55 57
Anti-CD40 AMAb90905 Monoclonal IHCWB 58 54
Anti-CHAT AMAb91130 Monoclonal IHCICC-IF 96 96
Anti-CHAT HPA048547 Polyclonal IHC 96 96
Anti-CHGA AMAb90525 Monoclonal IHCWBICC-IF 62 64
Anti-COX1 HPA002834 Polyclonal IHCWBICC-IF 93 90
Anti-COX2PTGS2 HPA001335 Polyclonal IHCICC-IF 88 88
Anti-CTSB HPA018156 Polyclonal IHCICC-IF 79 79
Anti-CTSD HPA003001 Polyclonal IHCWB 86 86
Anti-CXorf27 HPA003356 Polyclonal IHC 47 46
Anti-FBXO7 (PARK15) HPA032114 Polyclonal IHC 78 81
Anti-FUS AMAb90549 Monoclonal IHC WB ICC-IF 91 91Anti-GSK3B HPA028017 Polyclonal IHCWBICC-IF 100 100Anti-HIP1 HPA017964 Polyclonal IHCWB 79 77Anti-HTRA2 (PARK13) HPA027366 Polyclonal IHCWB 57 63Anti-ITGAM (CD11b) AMAb90911 Monoclonal IHCWB 67 68
Anti-ITM2B HPA029292 Polyclonal IHC ICC-IF 95 96Anti-LRP2 HPA005980 Polyclonal IHC 78 36
Products with enhanced validation for indicated application (see page 16)
13
Product Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-NFKB1 HPA027305 Polyclonal IHCWBICC-IF 60 62
Anti-OPTN HPA003360 Polyclonal IHCWBICC-IF 64 68
Anti-PADI4 HPA017007 Polyclonal IHCWB 66 69
Anti-PARK7 HPA004190 Polyclonal IHCWB 89 90
Anti-PFN1 AMAb91181 Monoclonal IHCWB 87 87
Anti-PHGDH AMAb90786 Monoclonal IHCWBICC-IF 99 99
Anti-PHGDH HPA021241 Polyclonal IHCWBICC-IF 99 99
Anti-PRNP HPA042754 Polyclonal IHC 91 91
Anti-PSEN1 HPA030760 Polyclonal IHC 82 81
Anti-RHOT1 AMAb90852 Monoclonal IHCWB 100 100
Anti-RHOT1 HPA010687 Polyclonal IHC 100 100
Anti-S100A8 HPA024372 Polyclonal IHCWBICC-IF 56 60
Anti-SERPINA3 HPA002560 Polyclonal IHCWB 60 59
Anti-SNCB HPA035876 Polyclonal IHCWB 97 97
Anti-SOD1 HPA001401 Polyclonal IHCWBICC-IF 82 81
Anti-SOD2 HPA001814 Polyclonal IHCWB 88 87
Anti-THY1 AMAb90844 Monoclonal IHCWB 64 68
Anti-THY1 HPA003733 Polyclonal IHCICC-IF 64 68
Anti-TNFRSF21 HPA006746 Polyclonal IHCWB 86 85
Anti-TTR AMAb90649 Monoclonal IHC WB 81 83
Anti-UBE2K HPA028869 Polyclonal IHCICC-IF 100 100
Anti-UCHL1 HPA005993 Polyclonal IHCWBICC-IF 97 97
Anti-USP46 AMAb90722 Monoclonal WBICC-IF 100 99
Anti-VPS26A AMAb90967 Monoclonal IHC WBICC-IF 96 100
Anti-WHSC1 AMAb90851 Monoclonal IHCWBICC-IF 91 91
Anti-WHSC1 HPA015801 Polyclonal IHCICC-IF 91 91
Anti-VWF AMAb90928 Monoclonal IHCWB 80 80
Anti-VWF HPA002082 Polyclonal IHC 82 78
The development of the central nervous system includes the development of the brain spinal cord optic and auditory systems
Within the neural tube stem cells generate the two major classes of cells that make the majority of the nervous system neurons and glia Both these classes of cells differentiate into many different types generated with highly specialized functions and shapes
The development of the peripheral and central nervous systems begins early in embryogenesis and can be traced through the use of lineage antibodies specific to each stage of neuronal development
The (SOX) family of transcription factors are well-established regulators of cell fate decisions during development
Vimentin (VIM) is a type III intermediate filament protein expressed in mesenchymal cells It is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both healthy development and metastatic progression
The cell surface glycoprotein Thy1 (CD90) is expressed on many mammalian cell types including neurons and serves as a human mesenchymal stem cell (MSC) marker
DEVELOPMENT
A
nti-VIM antibody
A
nti-SOX4 antibody
Anti-T
HY1 antibody
Anti-VIM polyclonal antibody (HPA001762) immunofluorescent staining of human cell line
ASC TERT1 shows localization to intermediate filaments in green
Anti-SOX4 monoclonal antibody (AMAb913802) immunofluorescent staining in mouse
hippocampus showing immunoreactivity in a subset of neurons in the granule cell layer of
the dentate gyrus in brown
Anti-THY1 polyclonal antibody (HPA003733) staining of human cerebral cortex shows
localization to neuropils in brown
Product Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-ENG AMAb90925 Monoclonal IHC 66 22Anti-ENG HPA011862 Polyclonal IHCWB 66 22Anti-FABP7 AMAb90595 Monoclonal IHCWB 89 90Anti-FABP7 HPA028825 Polyclonal IHCWB 89 90Anti-FLT1 AMAb90704 Monoclonal IHCWB 80 82Anti-GAP43 HPA015600 Polyclonal IHC ICC-IF 71 70Anti-GLI3 HPA005534 Polyclonal IHC 74 76Anti-MEF2C AMAb90727 Monoclonal IHCWBICC-IF 97 47Anti-MEF2C HPA005533 Polyclonal IHCWBICC-IF 97 47Anti-MKI67 (Ki67) HPA000451 Polyclonal IHCICC-IF 66 67Anti-NACC1 HPA021238 Polyclonal IHCICC-IF 91 89Anti-NES (Nestin) AMAb90556 Monoclonal IHCWBICC-IF 47 42Anti-NES (Nestin) HPA007007 Polyclonal IHCWB 47 42Anti-NKX2-2 HPA003468 Polyclonal IHCWB 96 97Anti-PAX6 HPA030775 Polyclonal IHCICC-IF 100 100Anti-PBK HPA005753 Polyclonal IHCWBICC-IF 91 94Anti-REST AMAb90740 Monoclonal IHCICC-IF 41 43Anti-RUNX1 HPA004176 Polyclonal IHCICC-IF 93 93Anti-RUNX2 AMAb90591 Monoclonal IHCWBICC-IF 100 81Anti-RUNX2 HPA022040 Polyclonal IHCWBICC-IF 100 81Anti-SATB2 AMAb90679 Monoclonal IHCWBICC-IF 100 100Anti-SATB2 HPA029543 Polyclonal IHCWBICC-IF 100 100Anti-SOX4 HPA029901 Polyclonal IHCICC-IF 100 39Anti-SOX6 HPA001923 Polyclonal IHCICC-IF 96 96Anti-SOX7 HPA009065 Polyclonal IHCWB 91 91Anti-SOX9 AMAb90795 Monoclonal IHCWBICC-IF 97 96Anti-SOX11 AMAb90502 Monoclonal IHCWB 82 82Anti-SOX11 HPA000536 Polyclonal IHCWB 82 82Anti-SOX30 HPA006159 Polyclonal IHCWB 68 70Anti-THY1 AMAb90844 Monoclonal IHCWB 64 68Anti-THY1 HPA003733 Polyclonal IHCICC-IF 64 68Anti-TM4SF2TSPAN7 HPA003140 Polyclonal IHCWB 96 96Anti-TM4SF2TSPAN7 AMAb90621 Monoclonal IHCWB 96 96Anti-VANGL1 AMAb90600 Monoclonal WBICC-IF 95 95Anti-VIM AMAb90516 Monoclonal IHCWB 99 99Anti-VIM HPA001762 Polyclonal IHCWBICC-IF 99 99
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Development
Products with enhanced validation for indicated application (see page 16)
15
At Atlas Antibodies we extensively validate our antibodies in IHC WB and ICC-IF Enhanced Validation is performed as an additional layer of security in an application and context-specific manner Enhanced validation offers increased security of antibody specificity in a defined context This is ensured by using the most relevant validation method for each combination of protein sample and application Enhanced Validation follows the guidelines proposed by the International Working Group for Antibody Validation (IWGAV)
ENHANCED VALIDATION
GENETIC VALIDATION Antibody specificity is confirmed by downregulating the target protein on a genetic level using siRNA
ORTHOGONAL VALIDATIONThe antibody is validated by comparing the results with a non-antibody based method across multiple samples
VALIDATION BY INDEPENDENT ANTIBODIESThe antibody specificity is demonstrated by comparing two antibodies targeting different regions of the same protein
RECOMBINANT EXPRESSION VALIDATIONThe antibody binding is verified using and over-expressed or tagged version of the target protein
MIGRATION CAPTURE MS VALIDATIONThe staining pattern and the protein size detected by the antibody is compared with results obtained by a capture Mass Spectrometry (MS) method
16
Triple A PolyclonalsTM are rabbit polyclonal primary antibodies developed within the Human Protein Atlas project IHC characterization data from 44 normal and 20 cancer tissues is available on the Human Protein Atlas portal
PrecisA MonoclonalsTM are mouse monoclonal primary antibodies developed against a number of carefully selected targets Clones are selected to recognize only unique non-overlapping epitopes and isotypes
Atlas Antibodies ABVoltavaumlgen 13SE-168 69 BrommaSwedenatlasantibodiescom
Phone +46(0)8 54 59 58 50contactatlasantibodiescomorderatlasantibodiescomsupportatlasantibodiescom
Atlas Antibodies logo Triple A Polyclonals PrecisA Monoclonals PrEST Antigen and QPrEST are trademarks or registered trademarks of Atlas Antibodies AB All other trademarks are the property of their respective owners
Products are for research use only Not for use in diagnostic procedures copy Atlas Antibodies AB 2020 008_
Neu
ro M
arke
r Pan
el _
Rev
03_2
0121
5_S
fN E
ditio
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VERY RELIABLE ANTIBODIESAtlas Antibodies is the original manufacturer of over 21000 primary antibodies targeting the majority of human proteins Our Triple A PolyclonalsTM and PrecisA MonoclonalsTM antibodies are available worldwide Building on our heritage with the Human Protein Atlas project we provide highly validated reagents that enable leading research in biology diagnostics and medicine for the purpose of understanding and improving human health
CREATED BY THE HUMAN PROTEIN ATLASTriple A Polyclonals are developed within the prestigious Human Protein Atlas project Our antibodies are validated in all major human tissues and organs and 20 cancer tissues Each antibody is supported by over 500 staining images
VALIDATED BY ENHANCED VALIDATIONWe take great care to validate our antibodies in IHC WB and ICC-IF As an additional layer of security we perform Enhanced Validation By using 5 different enhanced validation methods we validate our antibodies for each combination of protein sample and application
EVIDENCED BY SCIENCEOur antibodies are used all over the world and referenced in 1000s of scientific peer-reviewed papers
4
WHY ATLAS ANTIBODIES
The Human Protein Atlas
Atlas Antibodies
Learn more
NEUROINFLAMMATIONNeuroinflammation broadly defines the collective reactive immune response in the brain and spinal cord in response to injury and disease Inflammation in the central nervous system (CNS) is commonly associated with various degrees of tissue damage such as loss of myelin and neurons
The neuroinflammatory process is complex and involves disruption of the blood-brain barrier peripheral leukocyte infiltration edema and gliosis The inflammatory response is characterized by a host of cellular and molecular aberrations within the brain
Neuroinflammation arises locally within the CNS through phenotypic changes of different non-neuronal cell types in the brain such as microglia oligodendrocytes and astrocytes causing the release of different cytokines and chemokines and finally recruitment and infiltration of peripheral blood cells mainly T-and B-cells into the brain parenchyma
Neuroinflammatory processes are the key causative factors behind the brain and spinal cord injury This is true not only for acute brain trauma and hypoxic-ischemic brain damage following stroke but also for chronic infection and neurodegenerative diseases such as Alzheimerrsquos disease (AD) amyotrophic lateral sclerosis (ALS) Lewy body dementia (LBD) and multiple sclerosis (MS) In addition local peritumoral inflammation plays a role in the clinical progression and malignancy of glioblastomas the most aggressive primary brain tumors
Ant
i-GFA
P Anti-MBP Anti-P2RY12
Anti-
HLA-DRA antibody
Anti-
HLA-DRA Anti-GFAP
Anti-HLA-DRA (AMAb91674) shows microglial cells in red Anti-GFAP (AMAb91033) shows
astrocytes in green in Alzheimerrsquos disease cerebral cortex
Anti-P2RY12 (HPA014518) shows microglial cells in green Anti-GFAP(AMAb91033) shows
astrocytes in blue Anti-MBP (AMAb91062) shows myelinated processes in red in
Alzheimerrsquos disease cerebral cortex
Anti-HLA-DRA monoclonal antibody (AMAb91674) shows strong positivity in
microglial cells in Alzheimerrsquos disease cerebral cortex
6
New PrecisA MonoclonalsTM for Neuroinflammation
Products with enhanced validation for indicated application (see page 16)
7
Product Name
Product Number Clonality Validated
ApplicationsAnti-AGER AMAb91634 Monoclonal IgG2a IHC
Anti-AGER AMAb91635 Monoclonal IgG1 IHC
Anti-AIF1 AMAb91671 Monoclonal IgG2b IHC
Anti-AIF1 AMAb91672 Monoclonal IgG1 IHC
Anti-AMIGO3 AMAb91677 Monoclonal IgG1 WB
Anti-ANO2 AMAb91641 Monoclonal IgG1 IHC
Anti-ANO2 AMAb91642 Monoclonal IgG1 IHC
Anti-CD163 AMAb91646 Monoclonal IgG1 IHCWB
Anti-CD163 AMAb91648 Monoclonal IgG1 IHC
Anti-CRYAB AMAb91661 Monoclonal IgG1 IHC WB ICC-IF
Anti-CRYAB AMAb91662 Monoclonal IgG2b IHC WB
Anti-CXCL13 AMAb91629 Monoclonal IgG1 IHC
Anti-DYSF AMAb91667 Monoclonal IgG1 IHC WB
Anti-GAP43 AMAb91664 Monoclonal IgG2b IHC
Anti-GAP43 AMAb91665 Monoclonal IgG1 IHC
Anti-GAP43 AMAb91666 Monoclonal IgG2a IHC
Anti-GPR17 AMAb91624 Monoclonal IgG1 IHC WB
Anti-GZMB AMAb91650 Monoclonal IgG1 IHC
Anti-HLA-DRA AMAb91673 Monoclonal IgG1 IHC WB
Anti-HLA-DRA AMAb91674 Monoclonal IgG2a IHC WB
Anti-HLA-DRA AMAb91675 Monoclonal IgG2b IHC WB
Anti-IL17A AMAb91615 Monoclonal IgG1 IHC
Anti-IL17RA AMAb91617 Monoclonal IgG2b WB
Anti-IL17RA AMAb91619 Monoclonal IgG1 WB
Anti-IL7 AMAb91684 Monoclonal IgG1 ICC-IF
Anti-ITGA4 AMAb91699 Monoclonal IgG2b WB
Anti-MS4A1 AMAb91636 Monoclonal IgG2a IHC WB
Anti-OPALIN AMAb91685 Monoclonal IgG1 IHC
Anti-OPALIN AMAb91686 Monoclonal IgG2a IHC
Anti-PLP1 AMAb91639 Monoclonal IgG1 IHC
Anti-RGMA AMAb91702 Monoclonal IgG2b WB
Anti-S100A8 AMAb91689 Monoclonal IgG1 IHC
Anti-S100A9 AMAb91690 Monoclonal IgG2a IHC
Anti-SERPINA3 AMAb91655 Monoclonal IgG1 IHC
Anti-SPP1 AMAb91653 Monoclonal IgG1 IHC
Anti-TLR2 AMAb91631 Monoclonal IgG1 WB
Anti-TRPM4 AMAb91693 Monoclonal IgG1 IHC WB ICC-IF
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Anti-AGER HPA064436 Polyclonal WB ICC-IF 41 41
Anti-AIF1 HPA049234 Polyclonal IHC 84 84
Anti-AIF1 HPA062949 Polyclonal ICC-IF 86 83
Anti-ANO2 HPA057499 Polyclonal IHC 84 85
Anti-CD163 HPA046404 Polyclonal IHC ICC-IF 51 30
Anti-CD163 HPA051974 Polyclonal IHC 75 83
Anti-CRYAB HPA057100 Polyclonal IHC 98 98
Anti-DYSF HPA017071 Polyclonal IHC ICC-IF 96 96
Anti-DYSF HPA021945 Polyclonal IHC 88 88
Anti-GAP43 HPA013392 Polyclonal IHC ICC-IF 88 86
Anti-GAP43 HPA015600 Polyclonal IHC ICC-IF 71 70
Anti-GPR17 HPA029766 Polyclonal IHC 92 92
Anti-GZMB HPA003418 Polyclonal IHC WB 63 63
Anti-HLA-DRA HPA050162 Polyclonal IHC 54 69
Anti-HLA-DRA HPA053176 Polyclonal IHC 41 83
Anti-IL7 HPA019590 Polyclonal IHC 46 46
Anti-ITGA4 HPA074961 Polyclonal ICC-IF 90 89
Anti-MS4A1 HPA014341 Polyclonal IHC WB ICC-IF 73 77
Anti-MS4A1 HPA014391 Polyclonal IHC WB 61 64
Anti-OPALIN HPA014372 Polyclonal IHC 72 75
Anti-PLP1 HPA004128 Polyclonal IHC 100 100
Anti-RGMA HPA039880 Polyclonal ICC-IF 96 96
Anti-RGMA HPA044222 Polyclonal ICC-IF 87 87
Anti-S100A8 HPA024372 Polyclonal IHC WB ICC-IF 56 60
Anti-SERPINA3 HPA000893 Polyclonal IHC WB 61 61
Anti-SERPINA3 HPA002560 Polyclonal IHC WB 60 59
Anti-SPP1 HPA027541 Polyclonal IHC WB ICC-IF 56 53
Anti-TRPM4 HPA041169 Polyclonal IHC ICC-IF 93 91
NEW PRODUCTS
Selected Triple A PolyclonalsTM for Neuroinflammation
Products with enhanced validation for indicated application (see page 16)
Synaptic signaling is cell-cell communication that occurs at the synaptic level Cells communicate with each other via direct contact (juxtacrine signaling) over short distances (paracrine signaling) or vast distances and scales (endocrine signaling) Other cells form gap junctions that connect their cytoplasm to the cytoplasm of adjacent cells
When the sending neuron fires an electrical impulse travels along the axon As soon as the impulse reaches the synapse it triggers the release of neurotransmitters
The predominant excitatory neurotransmitter in the brain is glutamate (GLU) The inhibitory neurotransmitters GABA (in the brain) and glycine (in the brainstem and spinal cord) are common amino acids that participate in multiple metabolic cycles
Calcium (Ca2+) signaling in neurons is critical in cell signalingIt defines the ability of cells to release neurotransmitters thus regulating the intracellular signaling pathways Calbindin 2 (also known as calretinin) is one of the Ca2+-binding proteins involved in Ca2+ signaling that facilitates cytosolic Ca2+ diffusion without interfering with Ca2+ signaling
SYNAPTIC SIGNALING
A
nti-CALB2 antibody
An
ti-mGluR1 antibody
A
nti-VGAT antibody
Anti-VGAT monoclonal antibody (AMAb91043) shows strong immunoreactivity in GABAergic
fibers in the rat globus pallidus in green
Anti-CALB2 polyclonal antibody (HPA007305) shows the labeling in green of mouse
hippocampus Note the strong labeling in the dentate gyrus
Anti-MGLUR1 polyclonal antibody (HPA015701) strongly labels cortical perikarya in human
cerebral cortex tissue in brown
8
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Synaptic Signaling
9
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Anti-ATF2 HPA022134 Polyclonal IHC WB ICC-IF 99 99
Anti-ATF3 AMAb90909 Monoclonal IHC ICC-IF 92 92
Anti-ATF3 HPA001562 Polyclonal IHC ICC-IF 92 92
Anti-ATP1B1 HPA012911 Polyclonal IHC WB 93 93
Anti-ATP1B2 HPA010698 Polyclonal IHC 96 88
Anti-CAMK2B HPA026307 Polyclonal IHC WB 96 96
Anti-CALB1 HPA023099 Polyclonal IHC WB ICC-IF 98 99
Anti-CALB2 HPA007306 Polyclonal IHC WB ICC-IF 100 100
Anti-CALB2 HPA007305 Polyclonal IHC WB ICC-IF 100 100
Anti-CAMK2D HPA026281 Polyclonal IHC 100 97
Anti-C-FOS HPA018531 Polyclonal IHC ICC-IF 94 94
Anti-CHAT AMAb91130 Monoclonal IHC ICC-IF 96 96
Anti-CHAT HPA048547 Polyclonal IHC 96 96
Anti-CHRM1 (M1 mAChR) HPA014101 Polyclonal IHC 98 97
Anti-CHRM2 (M2 AChR) HPA029795 Polyclonal IHC 88 86
Anti-CLIC4 HPA008019 Polyclonal IHC WB 98 97
Anti-CREB1 HPA019150 Polyclonal IHC WB ICC-IF 100 100
Anti-DDC AMAb91089 Monoclonal IHCWB 90 88
Anti-DDC HPA017742 Polyclonal IHCWBICC-IF 90 88
Anti-EAAC1 HPA020086 Polyclonal IHC WB 77 79
Anti-EAAT2 HPA009172 Polyclonal IHC 87 89
Anti-GABRA3 HPA000839 Polyclonal IHCWB 91 93
Anti-GABRB1 HPA051297 Polyclonal IHC 97 100
Anti-GABRG1 HPA035622 Polyclonal IHC 96 94
Anti-GAD1 (GAD67) AMAb91076 Monoclonal IHCWB 94 94
Anti-GAD1 HPA058412 Polyclonal IHCWB 94 94
Anti-GAD2 AMAb91048 Monoclonal IHCWB 84 88
Anti-GAD2 HPA044637 Polyclonal IHC 84 88
Anti-GAT1 HPA013341 Polyclonal IHC WB 98 98
Anti-GAT3 HPA037981 Polyclonal IHCWB 85 87
Anti-GLUR2 (AMPA2) HPA008441 Polyclonal IHC 100 100
Anti-HTR2A HPA014011 Polyclonal IHC 95 97
Anti-KCC4 HPA041652 Polyclonal IHCWB 84 82
Anti-KCNJ5 (KIR34) HPA017353 Polyclonal IHCWB 89 89
Anti-KIF11 HPA010568 Polyclonal IHCWB 88 83
Anti-KIF17 HPA032085 Polyclonal IHCICC-IF 85 82
Anti-KIF18A HPA039312 Polyclonal IHCWBICC-IF 80 82
Anti-KIF1C HPA024602 Polyclonal IHCWB 81 83
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Anti-KIF26B HPA028562 Polyclonal IHCICC-IF 88 80
Anti-KIF4A (KIF4A amp B) HPA034745 Polyclonal IHCWBICC-IF 64 63
Anti-KIF5A HPA004469 Polyclonal IHCWB 91 88
Anti-KIF5C HPA035210 Polyclonal IHCWB 100100
Anti-KIF7 HPA043145 Polyclonal IHC 69 69
Anti-KIFAP3 HPA023742 Polyclonal IHC 100 100
Anti-MAPK1 (ERK) HPA030069 Polyclonal IHCWB 100 100
Anti-MAPK3 (ERK1) HPA005700 Polyclonal IHCWB 98 98
Anti-MGLUR1 HPA015701 Polyclonal IHC 80 80
Anti-MGLUR8 HPA051481 Polyclonal IHC 95 90
Anti-NCS1 HPA019713 Polyclonal IHCWBICC-IF 100 100
Anti-PRKCA HPA006563 Polyclonal IHCWBICC-IF 99 99
Anti-PRKCH HPA053709 Polyclonal IHCICC-IF 97 64
Anti-PNMT HPA051005 Polyclonal IHC 89 92
Anti-PRKACB (PKACB) HPA029754 Polyclonal IHC 73 76
Anti-PRKCZ HPA021851 Polyclonal IHCWB ICC-IF 94 94
Anti-RAB3A HPA003160 Polyclonal IHC 99 99
Anti-RAP1GAP HPA001922 Polyclonal IHC 92 91
Anti-SLC17A7 (VGLUT1) AMAb91041 Monoclonal IHCWB 94 94
Anti-SLC22A2 HPA008567 Polyclonal IHC 84 77
Anti-SLC32A1 (VGAT) HPA058859 Polyclonal IHC 95 93
Anti-SLC6A2 (NET) AMAb91116 Monoclonal IHC -
Anti-SLC6A3 (DAT) AMAb91125 Monoclonal IHC 85 85
Anti-SNAP25 HPA001830 Polyclonal IHCWB 100 100
Anti-SNAP29 HPA031823 Polyclonal IHC 89 92
Anti-SST (SOM) HPA019472 Polyclonal IHCWB 98 98
Anti-STXBP1 HPA008209 Polyclonal IHCWBICC-IF 100 100
Anti-STXBP6 HPA003552 Polyclonal IHCWB 99 99
Anti-SYNGR1 HPA029673 Polyclonal IHC ICC-IF 90 88
Anti-SYNPR HPA061671 Polyclonal IHC 97 97
Anti-SYP HPA002858 Polyclonal IHCWB 83 83
Anti-SYT1 HPA008394 Polyclonal IHCWB 100 100
Anti-SYT12 HPA011006 Polyclonal IHC 96 98
Anti-SYT13 HPA046224 Polyclonal IHC 96 93
Anti-SYT16 HPA004199 Polyclonal IHCWB 95 95
Anti-TGFA HPA042297 Polyclonal IHC 93 93
Anti-TH AMAb91112 Monoclonal IHC 88 88
Anti-TH HPA061003 Polyclonal IHC WB 88 88
Anti-TPH2 (NTPH) AMAb91108 Monoclonal IHC 100 100
Anti-VAMP4 HPA050418 Polyclonal IHCICC-IF 100 100
Anti-VAMP7 HPA036733 Polyclonal IHCICC-IF 98 93
Products with enhanced validation for indicated application (see page 16)
NEURAL LINEAGE
A
nti-GFAP antibody
Anti-C
NP antibody
Anti-N
EFM antibodyDuring central nervous system development neural stem cells (NSCs) proliferate and divide to generate a progeny that differentiate into neurons astrocytes oligodendrocytes and different glial cells
NSCs self-renewal and differentiation are regulated by a precise temporal sequence of growth factors intracellular signaling and transcription factor expression NSCs and their differentiated progeny can be identified by the expression of a unique combination of cell surface markers and transcription factors
The glial fibrillary acidic protein (GFAP) is an intermediate filament (IF) protein and a well-known marker for astrocytes
Oligodendrocytes can convert extracellular 2rsquo3rsquo-cAMP to adenosine As CNPase is the predominant enzyme involved in this conversion the cyclic nucleotide phosphodiesterase (CNP) is widely used as an oligodendrocyte marker
Neurons express the neurofilament medium (NFEM) polypeptide other intermediate filament proteins including nestin synemin syncoilin and vimentin
Anti-GFAP polyclonal antibody (HPA056030) shows strong immunoreactivity in the
human cerebellum in brown
Anti-NEFM polyclonal antibody (HPA022845) shows positivity in a subset of neuronal cells in
human cerebral cortex in brown
Anti-CNP polyclonal antibody (HPA023266) in the rat cerebral cortex is shown in magenta
10
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Neural Lineage
Products with enhanced validation for indicated application (see page 16)
11
TargetCells
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Astrocytes Anti-GFAP AMAb91033 Monoclonal IHC WB 98 100
Astrocytes Anti-GFAP HPA056030 Polyclonal IHCWB ICC-IF 98 100
Astrocytes Anti-GLUL AMAb91101 Monoclonal IHC WB 95 53
Astrocytes Anti-GLUL AMAb91102 Monoclonal IHC WB 95 92
Astrocytes Anti-GLUL AMAb91103 Monoclonal IHC WB 95 92
Astrocytes Anti-GLUL HPA007316 Polyclonal IHCWB 95 53
Astrocytes Anti-S100B AMAb91038 Monoclonal IHC WB 99 98
Astroglia Anti-EZR AMAb90976 Monoclonal IHCWBICC-IF 93 93
Astroglia Anti-EZR HPA021616 Polyclonal IHCWBICC-IF 93 93
Adhesion molecule Anti-ICAM5 HPA009083 Polyclonal IHC 85 86
Adhesion molecule Anti-MCAM HPA008848 Polyclonal IHC WB 75 73
Adhesion molecule Anti-NCAM2 HPA030900 Polyclonal IHC 89 91
Adhesion molecule Anti-NLGN1 HPA006680 Polyclonal IHCWB 98 98
Calcium binding protein Anti-CALB1 (CB) HPA023099 Polyclonal IHCWBICC-IF 98 99
Calcium binding protein Anti-CALB2 (CR) HPA007305 Polyclonal IHCWBICC-IF 100 100
Calcium binding proteins Anti-NECAB1 AMAb90801 Monoclonal IHCWB 98 98
Calcium binding protein Anti-NECAB1 HPA023629 Polyclonal IHCWB 98 98
Calcium binding protein Anti-NECAB2 AMAb90808 Monoclonal IHC 85 84
Calcium binding protein Anti-NECAB2 HPA013998 Polyclonal IHCICC-IF 98 97
Calcium binding protein Anti-SCGN AMAb90630 Monoclonal IHCWB 96 96
Calcium binding protein Anti-SCGN HPA006641 Polyclonal IHC 96 96
Calcium binding protein Anti-S100B HPA015768 Polyclonal IHCWBICC-IF 99 98
Oligodendrocytes Anti-MOG AMAb91066 Monoclonal IHC WB 91 89
Oligodendrocytes Anti-MOG AMAb91067 Monoclonal IHC WB 91 89
Oligodendrocytes Anti-MOG HPA021873 Polyclonal IHC 91 89
Oligodendrocytes Anti-CNP AMAb91068 Monoclonal IHC WB 76 77
Oligodendrocytes Anti-CNP AMAb91069 Monoclonal IHC WB 76 77
Oligodendrocytes Anti-CNP AMAb91072 Monoclonal IHC WB ICC-IF 76 77
Oligodendrocytes Anti-CNP HPA023280 Polyclonal IHCWBICC-IF 76 77
Progenitors Anti-PBK HPA005753 Polyclonal IHCWBICC-IF 91 94
Progenitors Anti-MKI67 (Ki67) AMAb90870 Monoclonal IHC ICC-IF 68 68
Progenitors Anti-MKI67 (Ki67) HPA000451 Polyclonal IHCICC-IF 66 67
TargetCells
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Cytoskeleton Anti-ACTN1 HPA006035 Polyclonal IHCWB 98 99
Cytoskeleton Anti-ACTN4 HPA001873 Polyclonal IHCWBICC-IF 99 98
Cytoskeleton Anti-INA HPA008057 Polyclonal IHC WB ICC-IF 83 84
Cytoskeleton Anti-MAP1A HPA039064 Polyclonal IHC 60 52
Cytoskeleton Anti-MAP1B HPA022275 Polyclonal IHCWB ICC-IF 85 86
Cytoskeleton Anti-MAP2 HPA008273 Polyclonal IHC WB ICC-IF 96 96
Cytoskeleton Anti-MYO5A HPA001356 Polyclonal IHCICC-IF 99 98
Cytoskeleton Anti-NEFM (NF160) HPA022845 Polyclonal IHCWBICC-IF 98 98
Microglia Anti-AIF HPA049234 Polyclonal IHC 84 84
Microglia Anti-CD68 AMAb90874 Monoclonal IHCWB 76 76
Microglia Anti-CD68 HPA048982 Polyclonal IHC WB 76 76
Microglia Anti-ITGAM (CD11b) AMAb90911 Monoclonal IHC WB 67 68
Microglia Anti-PTPRC AMAb90518 Monoclonal IHCWB 35 37
Microglia Anti-PTPRC HPA000440 Polyclonal IHCWB 35 37
Microglia Anti-TMEM119 AMAb91528 Monoclonal IHCWB ICC-IF 52 46
Microglia Anti-TMEM119 HPA051870 Polyclonal IHC 52 46
Neurons Anti-NEFM (NF160) AMAb91027 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91028 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91029 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91030 Monoclonal IHC WB 98 98
Neurons Anti-NEFH (NF200) AMAb91025 Monoclonal IHC WB 98 98
Neurons Anti-NEFH (NF200) HPA061615 Polyclonal IHC 88 94
Neurons Anti-UCHL1 AMAb91145 Monoclonal IHC WB ICC-IF 97 97
Neurons Anti-UCHL1 HPA005993 Polyclonal IHCWBICC-IF 97 97
Neurons Anti-VIM AMAb90516 Monoclonal IHCWB 99 99
Neurons Anti-VIM HPA001762 Polyclonal IHCWBICC-IF 99 99
Schwann cells Anti-MBP AMAb91062 Monoclonal IHC WB ICC-IF 97 97
Schwann cells Anti-MBP AMAb91063 Monoclonal IHC WB ICC-IF 97 97
Schwann cells Anti-MBP AMAb91064 Monoclonal IHC WB 97 97
Anti-I
TM2B antibodyNeurodegenerative diseases such as Amyotrophic Lateral Sclerosis Parkinsonrsquos Alzheimerrsquos and Huntingtonrsquos diseases occur as a result of the progressive loss of structure and function in neurons leading to the death of neurons
Age remains the primary risk factor for almost all the neurodegenerative disorders thus interfering with age-related molecular mechanisms or pathways might be an avenue to develop new therapeutics
The choline acetyltransferase (ChAT) is an enzyme responsible for catalyzing the synthesis of acetylcholine and is expressed in the majority of cholinergic neurons Hence ChAT immunoreactivity is used to detect cognitive decline in several neurodegenerative disorders
Integral membrane protein 2B (ITM2B) is a protein that in humans is encoded by the ITM2B gene This gene located on chromosome 13 is connected to familial dementia causing amyloid and pre-fibrillar effects similar to those seen in Alzheimerrsquos
The expression of the ubiquitin-carboxyl-terminal hydrolase isozyme L1 (UCHL1) present both in the neuronal cells of the central and peripheral nervous system is down-regulated in Parkinsonrsquos and Alzheimerrsquos disease patients
NEURODEGENERATIVE DISEASES
Anti-C
HAT antibody
Anti-U
CHL1 antibody
Anti-CHAT monoclonal antibody (AMAb91130) shows strong immunoreactivity in the
acetylcholine neurons in the rat diagonal band of Broca in green
Anti-ITM2B polyclonal antibody (HPA029292) targeting Integral membrane protein 2B strongly
labels Golgi apparatus in A-431 cells in green
Anti-UCHL1 monoclonal antibody (AMAb91145) in the mouse trigeminal ganglion show strong
immunoreactrivity in primary sensory neurons in green
12
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for NeurodegenerationProduct Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-ADAR AMAb90535 Monoclonal IHCWB ICC-IF 86 85
Anti-ADAR HPA003890 Polyclonal IHCWBICC-IF 86 85
Anti-AIMP1 HPA018476 Polyclonal IHCWBICC-IF 96 97
Anti-AKT1 AMAb90834 Monoclonal WB ICC-IF 97 97
Anti-AOX1 HPA040199 Polyclonal IHC 84 86
Anti-APBA3 HPA045577 Polyclonal IHC 70 68
Anti-APBB2 HPA023542 Polyclonal IHCWBICC-IF 85 83
Anti-APBB3 HPA005571 Polyclonal IHCICC-IF 84 84
Anti-APOA4 AMAb90769 Monoclonal IHC WB 65 28
Anti-APP HPA001462 Polyclonal IHCWB 95 95
Anti-AQP4 AMAb90537 Monoclonal IHCWB 93 92
Anti-AQP4 HPA014784 Polyclonal IHCWB ICC-IF 93 92
Anti-ATF2 HPA022134 Polyclonal IHCWBICC-IF 99 99
Anti-ATF3 AMAb90909 Monoclonal IHCICC-IF 92 92
Anti-ATF3 HPA001562 Polyclonal IHCICC-IF 92 92
Anti-ATRX AMAb90784 Monoclonal IHC WBICC-IF 96 97
Anti-ATXN1 HPA008335 Polyclonal IHC 81 81
Anti-ATXN2 HPA018295 Polyclonal IHCWBICC-IF 90 91
Anti-C3 HPA020432 Polyclonal IHC 78 23
Anti-CASP3 HPA002643 Polyclonal IHCWBICC-IF 84 88
Anti-CD4 AMAb90754 Monoclonal IHCWB 55 57
Anti-CD40 AMAb90905 Monoclonal IHCWB 58 54
Anti-CHAT AMAb91130 Monoclonal IHCICC-IF 96 96
Anti-CHAT HPA048547 Polyclonal IHC 96 96
Anti-CHGA AMAb90525 Monoclonal IHCWBICC-IF 62 64
Anti-COX1 HPA002834 Polyclonal IHCWBICC-IF 93 90
Anti-COX2PTGS2 HPA001335 Polyclonal IHCICC-IF 88 88
Anti-CTSB HPA018156 Polyclonal IHCICC-IF 79 79
Anti-CTSD HPA003001 Polyclonal IHCWB 86 86
Anti-CXorf27 HPA003356 Polyclonal IHC 47 46
Anti-FBXO7 (PARK15) HPA032114 Polyclonal IHC 78 81
Anti-FUS AMAb90549 Monoclonal IHC WB ICC-IF 91 91Anti-GSK3B HPA028017 Polyclonal IHCWBICC-IF 100 100Anti-HIP1 HPA017964 Polyclonal IHCWB 79 77Anti-HTRA2 (PARK13) HPA027366 Polyclonal IHCWB 57 63Anti-ITGAM (CD11b) AMAb90911 Monoclonal IHCWB 67 68
Anti-ITM2B HPA029292 Polyclonal IHC ICC-IF 95 96Anti-LRP2 HPA005980 Polyclonal IHC 78 36
Products with enhanced validation for indicated application (see page 16)
13
Product Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-NFKB1 HPA027305 Polyclonal IHCWBICC-IF 60 62
Anti-OPTN HPA003360 Polyclonal IHCWBICC-IF 64 68
Anti-PADI4 HPA017007 Polyclonal IHCWB 66 69
Anti-PARK7 HPA004190 Polyclonal IHCWB 89 90
Anti-PFN1 AMAb91181 Monoclonal IHCWB 87 87
Anti-PHGDH AMAb90786 Monoclonal IHCWBICC-IF 99 99
Anti-PHGDH HPA021241 Polyclonal IHCWBICC-IF 99 99
Anti-PRNP HPA042754 Polyclonal IHC 91 91
Anti-PSEN1 HPA030760 Polyclonal IHC 82 81
Anti-RHOT1 AMAb90852 Monoclonal IHCWB 100 100
Anti-RHOT1 HPA010687 Polyclonal IHC 100 100
Anti-S100A8 HPA024372 Polyclonal IHCWBICC-IF 56 60
Anti-SERPINA3 HPA002560 Polyclonal IHCWB 60 59
Anti-SNCB HPA035876 Polyclonal IHCWB 97 97
Anti-SOD1 HPA001401 Polyclonal IHCWBICC-IF 82 81
Anti-SOD2 HPA001814 Polyclonal IHCWB 88 87
Anti-THY1 AMAb90844 Monoclonal IHCWB 64 68
Anti-THY1 HPA003733 Polyclonal IHCICC-IF 64 68
Anti-TNFRSF21 HPA006746 Polyclonal IHCWB 86 85
Anti-TTR AMAb90649 Monoclonal IHC WB 81 83
Anti-UBE2K HPA028869 Polyclonal IHCICC-IF 100 100
Anti-UCHL1 HPA005993 Polyclonal IHCWBICC-IF 97 97
Anti-USP46 AMAb90722 Monoclonal WBICC-IF 100 99
Anti-VPS26A AMAb90967 Monoclonal IHC WBICC-IF 96 100
Anti-WHSC1 AMAb90851 Monoclonal IHCWBICC-IF 91 91
Anti-WHSC1 HPA015801 Polyclonal IHCICC-IF 91 91
Anti-VWF AMAb90928 Monoclonal IHCWB 80 80
Anti-VWF HPA002082 Polyclonal IHC 82 78
The development of the central nervous system includes the development of the brain spinal cord optic and auditory systems
Within the neural tube stem cells generate the two major classes of cells that make the majority of the nervous system neurons and glia Both these classes of cells differentiate into many different types generated with highly specialized functions and shapes
The development of the peripheral and central nervous systems begins early in embryogenesis and can be traced through the use of lineage antibodies specific to each stage of neuronal development
The (SOX) family of transcription factors are well-established regulators of cell fate decisions during development
Vimentin (VIM) is a type III intermediate filament protein expressed in mesenchymal cells It is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both healthy development and metastatic progression
The cell surface glycoprotein Thy1 (CD90) is expressed on many mammalian cell types including neurons and serves as a human mesenchymal stem cell (MSC) marker
DEVELOPMENT
A
nti-VIM antibody
A
nti-SOX4 antibody
Anti-T
HY1 antibody
Anti-VIM polyclonal antibody (HPA001762) immunofluorescent staining of human cell line
ASC TERT1 shows localization to intermediate filaments in green
Anti-SOX4 monoclonal antibody (AMAb913802) immunofluorescent staining in mouse
hippocampus showing immunoreactivity in a subset of neurons in the granule cell layer of
the dentate gyrus in brown
Anti-THY1 polyclonal antibody (HPA003733) staining of human cerebral cortex shows
localization to neuropils in brown
Product Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-ENG AMAb90925 Monoclonal IHC 66 22Anti-ENG HPA011862 Polyclonal IHCWB 66 22Anti-FABP7 AMAb90595 Monoclonal IHCWB 89 90Anti-FABP7 HPA028825 Polyclonal IHCWB 89 90Anti-FLT1 AMAb90704 Monoclonal IHCWB 80 82Anti-GAP43 HPA015600 Polyclonal IHC ICC-IF 71 70Anti-GLI3 HPA005534 Polyclonal IHC 74 76Anti-MEF2C AMAb90727 Monoclonal IHCWBICC-IF 97 47Anti-MEF2C HPA005533 Polyclonal IHCWBICC-IF 97 47Anti-MKI67 (Ki67) HPA000451 Polyclonal IHCICC-IF 66 67Anti-NACC1 HPA021238 Polyclonal IHCICC-IF 91 89Anti-NES (Nestin) AMAb90556 Monoclonal IHCWBICC-IF 47 42Anti-NES (Nestin) HPA007007 Polyclonal IHCWB 47 42Anti-NKX2-2 HPA003468 Polyclonal IHCWB 96 97Anti-PAX6 HPA030775 Polyclonal IHCICC-IF 100 100Anti-PBK HPA005753 Polyclonal IHCWBICC-IF 91 94Anti-REST AMAb90740 Monoclonal IHCICC-IF 41 43Anti-RUNX1 HPA004176 Polyclonal IHCICC-IF 93 93Anti-RUNX2 AMAb90591 Monoclonal IHCWBICC-IF 100 81Anti-RUNX2 HPA022040 Polyclonal IHCWBICC-IF 100 81Anti-SATB2 AMAb90679 Monoclonal IHCWBICC-IF 100 100Anti-SATB2 HPA029543 Polyclonal IHCWBICC-IF 100 100Anti-SOX4 HPA029901 Polyclonal IHCICC-IF 100 39Anti-SOX6 HPA001923 Polyclonal IHCICC-IF 96 96Anti-SOX7 HPA009065 Polyclonal IHCWB 91 91Anti-SOX9 AMAb90795 Monoclonal IHCWBICC-IF 97 96Anti-SOX11 AMAb90502 Monoclonal IHCWB 82 82Anti-SOX11 HPA000536 Polyclonal IHCWB 82 82Anti-SOX30 HPA006159 Polyclonal IHCWB 68 70Anti-THY1 AMAb90844 Monoclonal IHCWB 64 68Anti-THY1 HPA003733 Polyclonal IHCICC-IF 64 68Anti-TM4SF2TSPAN7 HPA003140 Polyclonal IHCWB 96 96Anti-TM4SF2TSPAN7 AMAb90621 Monoclonal IHCWB 96 96Anti-VANGL1 AMAb90600 Monoclonal WBICC-IF 95 95Anti-VIM AMAb90516 Monoclonal IHCWB 99 99Anti-VIM HPA001762 Polyclonal IHCWBICC-IF 99 99
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Development
Products with enhanced validation for indicated application (see page 16)
15
At Atlas Antibodies we extensively validate our antibodies in IHC WB and ICC-IF Enhanced Validation is performed as an additional layer of security in an application and context-specific manner Enhanced validation offers increased security of antibody specificity in a defined context This is ensured by using the most relevant validation method for each combination of protein sample and application Enhanced Validation follows the guidelines proposed by the International Working Group for Antibody Validation (IWGAV)
ENHANCED VALIDATION
GENETIC VALIDATION Antibody specificity is confirmed by downregulating the target protein on a genetic level using siRNA
ORTHOGONAL VALIDATIONThe antibody is validated by comparing the results with a non-antibody based method across multiple samples
VALIDATION BY INDEPENDENT ANTIBODIESThe antibody specificity is demonstrated by comparing two antibodies targeting different regions of the same protein
RECOMBINANT EXPRESSION VALIDATIONThe antibody binding is verified using and over-expressed or tagged version of the target protein
MIGRATION CAPTURE MS VALIDATIONThe staining pattern and the protein size detected by the antibody is compared with results obtained by a capture Mass Spectrometry (MS) method
16
Triple A PolyclonalsTM are rabbit polyclonal primary antibodies developed within the Human Protein Atlas project IHC characterization data from 44 normal and 20 cancer tissues is available on the Human Protein Atlas portal
PrecisA MonoclonalsTM are mouse monoclonal primary antibodies developed against a number of carefully selected targets Clones are selected to recognize only unique non-overlapping epitopes and isotypes
Atlas Antibodies ABVoltavaumlgen 13SE-168 69 BrommaSwedenatlasantibodiescom
Phone +46(0)8 54 59 58 50contactatlasantibodiescomorderatlasantibodiescomsupportatlasantibodiescom
Atlas Antibodies logo Triple A Polyclonals PrecisA Monoclonals PrEST Antigen and QPrEST are trademarks or registered trademarks of Atlas Antibodies AB All other trademarks are the property of their respective owners
Products are for research use only Not for use in diagnostic procedures copy Atlas Antibodies AB 2020 008_
Neu
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arke
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Rev
03_2
0121
5_S
fN E
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NEUROINFLAMMATIONNeuroinflammation broadly defines the collective reactive immune response in the brain and spinal cord in response to injury and disease Inflammation in the central nervous system (CNS) is commonly associated with various degrees of tissue damage such as loss of myelin and neurons
The neuroinflammatory process is complex and involves disruption of the blood-brain barrier peripheral leukocyte infiltration edema and gliosis The inflammatory response is characterized by a host of cellular and molecular aberrations within the brain
Neuroinflammation arises locally within the CNS through phenotypic changes of different non-neuronal cell types in the brain such as microglia oligodendrocytes and astrocytes causing the release of different cytokines and chemokines and finally recruitment and infiltration of peripheral blood cells mainly T-and B-cells into the brain parenchyma
Neuroinflammatory processes are the key causative factors behind the brain and spinal cord injury This is true not only for acute brain trauma and hypoxic-ischemic brain damage following stroke but also for chronic infection and neurodegenerative diseases such as Alzheimerrsquos disease (AD) amyotrophic lateral sclerosis (ALS) Lewy body dementia (LBD) and multiple sclerosis (MS) In addition local peritumoral inflammation plays a role in the clinical progression and malignancy of glioblastomas the most aggressive primary brain tumors
Ant
i-GFA
P Anti-MBP Anti-P2RY12
Anti-
HLA-DRA antibody
Anti-
HLA-DRA Anti-GFAP
Anti-HLA-DRA (AMAb91674) shows microglial cells in red Anti-GFAP (AMAb91033) shows
astrocytes in green in Alzheimerrsquos disease cerebral cortex
Anti-P2RY12 (HPA014518) shows microglial cells in green Anti-GFAP(AMAb91033) shows
astrocytes in blue Anti-MBP (AMAb91062) shows myelinated processes in red in
Alzheimerrsquos disease cerebral cortex
Anti-HLA-DRA monoclonal antibody (AMAb91674) shows strong positivity in
microglial cells in Alzheimerrsquos disease cerebral cortex
6
New PrecisA MonoclonalsTM for Neuroinflammation
Products with enhanced validation for indicated application (see page 16)
7
Product Name
Product Number Clonality Validated
ApplicationsAnti-AGER AMAb91634 Monoclonal IgG2a IHC
Anti-AGER AMAb91635 Monoclonal IgG1 IHC
Anti-AIF1 AMAb91671 Monoclonal IgG2b IHC
Anti-AIF1 AMAb91672 Monoclonal IgG1 IHC
Anti-AMIGO3 AMAb91677 Monoclonal IgG1 WB
Anti-ANO2 AMAb91641 Monoclonal IgG1 IHC
Anti-ANO2 AMAb91642 Monoclonal IgG1 IHC
Anti-CD163 AMAb91646 Monoclonal IgG1 IHCWB
Anti-CD163 AMAb91648 Monoclonal IgG1 IHC
Anti-CRYAB AMAb91661 Monoclonal IgG1 IHC WB ICC-IF
Anti-CRYAB AMAb91662 Monoclonal IgG2b IHC WB
Anti-CXCL13 AMAb91629 Monoclonal IgG1 IHC
Anti-DYSF AMAb91667 Monoclonal IgG1 IHC WB
Anti-GAP43 AMAb91664 Monoclonal IgG2b IHC
Anti-GAP43 AMAb91665 Monoclonal IgG1 IHC
Anti-GAP43 AMAb91666 Monoclonal IgG2a IHC
Anti-GPR17 AMAb91624 Monoclonal IgG1 IHC WB
Anti-GZMB AMAb91650 Monoclonal IgG1 IHC
Anti-HLA-DRA AMAb91673 Monoclonal IgG1 IHC WB
Anti-HLA-DRA AMAb91674 Monoclonal IgG2a IHC WB
Anti-HLA-DRA AMAb91675 Monoclonal IgG2b IHC WB
Anti-IL17A AMAb91615 Monoclonal IgG1 IHC
Anti-IL17RA AMAb91617 Monoclonal IgG2b WB
Anti-IL17RA AMAb91619 Monoclonal IgG1 WB
Anti-IL7 AMAb91684 Monoclonal IgG1 ICC-IF
Anti-ITGA4 AMAb91699 Monoclonal IgG2b WB
Anti-MS4A1 AMAb91636 Monoclonal IgG2a IHC WB
Anti-OPALIN AMAb91685 Monoclonal IgG1 IHC
Anti-OPALIN AMAb91686 Monoclonal IgG2a IHC
Anti-PLP1 AMAb91639 Monoclonal IgG1 IHC
Anti-RGMA AMAb91702 Monoclonal IgG2b WB
Anti-S100A8 AMAb91689 Monoclonal IgG1 IHC
Anti-S100A9 AMAb91690 Monoclonal IgG2a IHC
Anti-SERPINA3 AMAb91655 Monoclonal IgG1 IHC
Anti-SPP1 AMAb91653 Monoclonal IgG1 IHC
Anti-TLR2 AMAb91631 Monoclonal IgG1 WB
Anti-TRPM4 AMAb91693 Monoclonal IgG1 IHC WB ICC-IF
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Anti-AGER HPA064436 Polyclonal WB ICC-IF 41 41
Anti-AIF1 HPA049234 Polyclonal IHC 84 84
Anti-AIF1 HPA062949 Polyclonal ICC-IF 86 83
Anti-ANO2 HPA057499 Polyclonal IHC 84 85
Anti-CD163 HPA046404 Polyclonal IHC ICC-IF 51 30
Anti-CD163 HPA051974 Polyclonal IHC 75 83
Anti-CRYAB HPA057100 Polyclonal IHC 98 98
Anti-DYSF HPA017071 Polyclonal IHC ICC-IF 96 96
Anti-DYSF HPA021945 Polyclonal IHC 88 88
Anti-GAP43 HPA013392 Polyclonal IHC ICC-IF 88 86
Anti-GAP43 HPA015600 Polyclonal IHC ICC-IF 71 70
Anti-GPR17 HPA029766 Polyclonal IHC 92 92
Anti-GZMB HPA003418 Polyclonal IHC WB 63 63
Anti-HLA-DRA HPA050162 Polyclonal IHC 54 69
Anti-HLA-DRA HPA053176 Polyclonal IHC 41 83
Anti-IL7 HPA019590 Polyclonal IHC 46 46
Anti-ITGA4 HPA074961 Polyclonal ICC-IF 90 89
Anti-MS4A1 HPA014341 Polyclonal IHC WB ICC-IF 73 77
Anti-MS4A1 HPA014391 Polyclonal IHC WB 61 64
Anti-OPALIN HPA014372 Polyclonal IHC 72 75
Anti-PLP1 HPA004128 Polyclonal IHC 100 100
Anti-RGMA HPA039880 Polyclonal ICC-IF 96 96
Anti-RGMA HPA044222 Polyclonal ICC-IF 87 87
Anti-S100A8 HPA024372 Polyclonal IHC WB ICC-IF 56 60
Anti-SERPINA3 HPA000893 Polyclonal IHC WB 61 61
Anti-SERPINA3 HPA002560 Polyclonal IHC WB 60 59
Anti-SPP1 HPA027541 Polyclonal IHC WB ICC-IF 56 53
Anti-TRPM4 HPA041169 Polyclonal IHC ICC-IF 93 91
NEW PRODUCTS
Selected Triple A PolyclonalsTM for Neuroinflammation
Products with enhanced validation for indicated application (see page 16)
Synaptic signaling is cell-cell communication that occurs at the synaptic level Cells communicate with each other via direct contact (juxtacrine signaling) over short distances (paracrine signaling) or vast distances and scales (endocrine signaling) Other cells form gap junctions that connect their cytoplasm to the cytoplasm of adjacent cells
When the sending neuron fires an electrical impulse travels along the axon As soon as the impulse reaches the synapse it triggers the release of neurotransmitters
The predominant excitatory neurotransmitter in the brain is glutamate (GLU) The inhibitory neurotransmitters GABA (in the brain) and glycine (in the brainstem and spinal cord) are common amino acids that participate in multiple metabolic cycles
Calcium (Ca2+) signaling in neurons is critical in cell signalingIt defines the ability of cells to release neurotransmitters thus regulating the intracellular signaling pathways Calbindin 2 (also known as calretinin) is one of the Ca2+-binding proteins involved in Ca2+ signaling that facilitates cytosolic Ca2+ diffusion without interfering with Ca2+ signaling
SYNAPTIC SIGNALING
A
nti-CALB2 antibody
An
ti-mGluR1 antibody
A
nti-VGAT antibody
Anti-VGAT monoclonal antibody (AMAb91043) shows strong immunoreactivity in GABAergic
fibers in the rat globus pallidus in green
Anti-CALB2 polyclonal antibody (HPA007305) shows the labeling in green of mouse
hippocampus Note the strong labeling in the dentate gyrus
Anti-MGLUR1 polyclonal antibody (HPA015701) strongly labels cortical perikarya in human
cerebral cortex tissue in brown
8
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Synaptic Signaling
9
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Anti-ATF2 HPA022134 Polyclonal IHC WB ICC-IF 99 99
Anti-ATF3 AMAb90909 Monoclonal IHC ICC-IF 92 92
Anti-ATF3 HPA001562 Polyclonal IHC ICC-IF 92 92
Anti-ATP1B1 HPA012911 Polyclonal IHC WB 93 93
Anti-ATP1B2 HPA010698 Polyclonal IHC 96 88
Anti-CAMK2B HPA026307 Polyclonal IHC WB 96 96
Anti-CALB1 HPA023099 Polyclonal IHC WB ICC-IF 98 99
Anti-CALB2 HPA007306 Polyclonal IHC WB ICC-IF 100 100
Anti-CALB2 HPA007305 Polyclonal IHC WB ICC-IF 100 100
Anti-CAMK2D HPA026281 Polyclonal IHC 100 97
Anti-C-FOS HPA018531 Polyclonal IHC ICC-IF 94 94
Anti-CHAT AMAb91130 Monoclonal IHC ICC-IF 96 96
Anti-CHAT HPA048547 Polyclonal IHC 96 96
Anti-CHRM1 (M1 mAChR) HPA014101 Polyclonal IHC 98 97
Anti-CHRM2 (M2 AChR) HPA029795 Polyclonal IHC 88 86
Anti-CLIC4 HPA008019 Polyclonal IHC WB 98 97
Anti-CREB1 HPA019150 Polyclonal IHC WB ICC-IF 100 100
Anti-DDC AMAb91089 Monoclonal IHCWB 90 88
Anti-DDC HPA017742 Polyclonal IHCWBICC-IF 90 88
Anti-EAAC1 HPA020086 Polyclonal IHC WB 77 79
Anti-EAAT2 HPA009172 Polyclonal IHC 87 89
Anti-GABRA3 HPA000839 Polyclonal IHCWB 91 93
Anti-GABRB1 HPA051297 Polyclonal IHC 97 100
Anti-GABRG1 HPA035622 Polyclonal IHC 96 94
Anti-GAD1 (GAD67) AMAb91076 Monoclonal IHCWB 94 94
Anti-GAD1 HPA058412 Polyclonal IHCWB 94 94
Anti-GAD2 AMAb91048 Monoclonal IHCWB 84 88
Anti-GAD2 HPA044637 Polyclonal IHC 84 88
Anti-GAT1 HPA013341 Polyclonal IHC WB 98 98
Anti-GAT3 HPA037981 Polyclonal IHCWB 85 87
Anti-GLUR2 (AMPA2) HPA008441 Polyclonal IHC 100 100
Anti-HTR2A HPA014011 Polyclonal IHC 95 97
Anti-KCC4 HPA041652 Polyclonal IHCWB 84 82
Anti-KCNJ5 (KIR34) HPA017353 Polyclonal IHCWB 89 89
Anti-KIF11 HPA010568 Polyclonal IHCWB 88 83
Anti-KIF17 HPA032085 Polyclonal IHCICC-IF 85 82
Anti-KIF18A HPA039312 Polyclonal IHCWBICC-IF 80 82
Anti-KIF1C HPA024602 Polyclonal IHCWB 81 83
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Anti-KIF26B HPA028562 Polyclonal IHCICC-IF 88 80
Anti-KIF4A (KIF4A amp B) HPA034745 Polyclonal IHCWBICC-IF 64 63
Anti-KIF5A HPA004469 Polyclonal IHCWB 91 88
Anti-KIF5C HPA035210 Polyclonal IHCWB 100100
Anti-KIF7 HPA043145 Polyclonal IHC 69 69
Anti-KIFAP3 HPA023742 Polyclonal IHC 100 100
Anti-MAPK1 (ERK) HPA030069 Polyclonal IHCWB 100 100
Anti-MAPK3 (ERK1) HPA005700 Polyclonal IHCWB 98 98
Anti-MGLUR1 HPA015701 Polyclonal IHC 80 80
Anti-MGLUR8 HPA051481 Polyclonal IHC 95 90
Anti-NCS1 HPA019713 Polyclonal IHCWBICC-IF 100 100
Anti-PRKCA HPA006563 Polyclonal IHCWBICC-IF 99 99
Anti-PRKCH HPA053709 Polyclonal IHCICC-IF 97 64
Anti-PNMT HPA051005 Polyclonal IHC 89 92
Anti-PRKACB (PKACB) HPA029754 Polyclonal IHC 73 76
Anti-PRKCZ HPA021851 Polyclonal IHCWB ICC-IF 94 94
Anti-RAB3A HPA003160 Polyclonal IHC 99 99
Anti-RAP1GAP HPA001922 Polyclonal IHC 92 91
Anti-SLC17A7 (VGLUT1) AMAb91041 Monoclonal IHCWB 94 94
Anti-SLC22A2 HPA008567 Polyclonal IHC 84 77
Anti-SLC32A1 (VGAT) HPA058859 Polyclonal IHC 95 93
Anti-SLC6A2 (NET) AMAb91116 Monoclonal IHC -
Anti-SLC6A3 (DAT) AMAb91125 Monoclonal IHC 85 85
Anti-SNAP25 HPA001830 Polyclonal IHCWB 100 100
Anti-SNAP29 HPA031823 Polyclonal IHC 89 92
Anti-SST (SOM) HPA019472 Polyclonal IHCWB 98 98
Anti-STXBP1 HPA008209 Polyclonal IHCWBICC-IF 100 100
Anti-STXBP6 HPA003552 Polyclonal IHCWB 99 99
Anti-SYNGR1 HPA029673 Polyclonal IHC ICC-IF 90 88
Anti-SYNPR HPA061671 Polyclonal IHC 97 97
Anti-SYP HPA002858 Polyclonal IHCWB 83 83
Anti-SYT1 HPA008394 Polyclonal IHCWB 100 100
Anti-SYT12 HPA011006 Polyclonal IHC 96 98
Anti-SYT13 HPA046224 Polyclonal IHC 96 93
Anti-SYT16 HPA004199 Polyclonal IHCWB 95 95
Anti-TGFA HPA042297 Polyclonal IHC 93 93
Anti-TH AMAb91112 Monoclonal IHC 88 88
Anti-TH HPA061003 Polyclonal IHC WB 88 88
Anti-TPH2 (NTPH) AMAb91108 Monoclonal IHC 100 100
Anti-VAMP4 HPA050418 Polyclonal IHCICC-IF 100 100
Anti-VAMP7 HPA036733 Polyclonal IHCICC-IF 98 93
Products with enhanced validation for indicated application (see page 16)
NEURAL LINEAGE
A
nti-GFAP antibody
Anti-C
NP antibody
Anti-N
EFM antibodyDuring central nervous system development neural stem cells (NSCs) proliferate and divide to generate a progeny that differentiate into neurons astrocytes oligodendrocytes and different glial cells
NSCs self-renewal and differentiation are regulated by a precise temporal sequence of growth factors intracellular signaling and transcription factor expression NSCs and their differentiated progeny can be identified by the expression of a unique combination of cell surface markers and transcription factors
The glial fibrillary acidic protein (GFAP) is an intermediate filament (IF) protein and a well-known marker for astrocytes
Oligodendrocytes can convert extracellular 2rsquo3rsquo-cAMP to adenosine As CNPase is the predominant enzyme involved in this conversion the cyclic nucleotide phosphodiesterase (CNP) is widely used as an oligodendrocyte marker
Neurons express the neurofilament medium (NFEM) polypeptide other intermediate filament proteins including nestin synemin syncoilin and vimentin
Anti-GFAP polyclonal antibody (HPA056030) shows strong immunoreactivity in the
human cerebellum in brown
Anti-NEFM polyclonal antibody (HPA022845) shows positivity in a subset of neuronal cells in
human cerebral cortex in brown
Anti-CNP polyclonal antibody (HPA023266) in the rat cerebral cortex is shown in magenta
10
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Neural Lineage
Products with enhanced validation for indicated application (see page 16)
11
TargetCells
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Astrocytes Anti-GFAP AMAb91033 Monoclonal IHC WB 98 100
Astrocytes Anti-GFAP HPA056030 Polyclonal IHCWB ICC-IF 98 100
Astrocytes Anti-GLUL AMAb91101 Monoclonal IHC WB 95 53
Astrocytes Anti-GLUL AMAb91102 Monoclonal IHC WB 95 92
Astrocytes Anti-GLUL AMAb91103 Monoclonal IHC WB 95 92
Astrocytes Anti-GLUL HPA007316 Polyclonal IHCWB 95 53
Astrocytes Anti-S100B AMAb91038 Monoclonal IHC WB 99 98
Astroglia Anti-EZR AMAb90976 Monoclonal IHCWBICC-IF 93 93
Astroglia Anti-EZR HPA021616 Polyclonal IHCWBICC-IF 93 93
Adhesion molecule Anti-ICAM5 HPA009083 Polyclonal IHC 85 86
Adhesion molecule Anti-MCAM HPA008848 Polyclonal IHC WB 75 73
Adhesion molecule Anti-NCAM2 HPA030900 Polyclonal IHC 89 91
Adhesion molecule Anti-NLGN1 HPA006680 Polyclonal IHCWB 98 98
Calcium binding protein Anti-CALB1 (CB) HPA023099 Polyclonal IHCWBICC-IF 98 99
Calcium binding protein Anti-CALB2 (CR) HPA007305 Polyclonal IHCWBICC-IF 100 100
Calcium binding proteins Anti-NECAB1 AMAb90801 Monoclonal IHCWB 98 98
Calcium binding protein Anti-NECAB1 HPA023629 Polyclonal IHCWB 98 98
Calcium binding protein Anti-NECAB2 AMAb90808 Monoclonal IHC 85 84
Calcium binding protein Anti-NECAB2 HPA013998 Polyclonal IHCICC-IF 98 97
Calcium binding protein Anti-SCGN AMAb90630 Monoclonal IHCWB 96 96
Calcium binding protein Anti-SCGN HPA006641 Polyclonal IHC 96 96
Calcium binding protein Anti-S100B HPA015768 Polyclonal IHCWBICC-IF 99 98
Oligodendrocytes Anti-MOG AMAb91066 Monoclonal IHC WB 91 89
Oligodendrocytes Anti-MOG AMAb91067 Monoclonal IHC WB 91 89
Oligodendrocytes Anti-MOG HPA021873 Polyclonal IHC 91 89
Oligodendrocytes Anti-CNP AMAb91068 Monoclonal IHC WB 76 77
Oligodendrocytes Anti-CNP AMAb91069 Monoclonal IHC WB 76 77
Oligodendrocytes Anti-CNP AMAb91072 Monoclonal IHC WB ICC-IF 76 77
Oligodendrocytes Anti-CNP HPA023280 Polyclonal IHCWBICC-IF 76 77
Progenitors Anti-PBK HPA005753 Polyclonal IHCWBICC-IF 91 94
Progenitors Anti-MKI67 (Ki67) AMAb90870 Monoclonal IHC ICC-IF 68 68
Progenitors Anti-MKI67 (Ki67) HPA000451 Polyclonal IHCICC-IF 66 67
TargetCells
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Cytoskeleton Anti-ACTN1 HPA006035 Polyclonal IHCWB 98 99
Cytoskeleton Anti-ACTN4 HPA001873 Polyclonal IHCWBICC-IF 99 98
Cytoskeleton Anti-INA HPA008057 Polyclonal IHC WB ICC-IF 83 84
Cytoskeleton Anti-MAP1A HPA039064 Polyclonal IHC 60 52
Cytoskeleton Anti-MAP1B HPA022275 Polyclonal IHCWB ICC-IF 85 86
Cytoskeleton Anti-MAP2 HPA008273 Polyclonal IHC WB ICC-IF 96 96
Cytoskeleton Anti-MYO5A HPA001356 Polyclonal IHCICC-IF 99 98
Cytoskeleton Anti-NEFM (NF160) HPA022845 Polyclonal IHCWBICC-IF 98 98
Microglia Anti-AIF HPA049234 Polyclonal IHC 84 84
Microglia Anti-CD68 AMAb90874 Monoclonal IHCWB 76 76
Microglia Anti-CD68 HPA048982 Polyclonal IHC WB 76 76
Microglia Anti-ITGAM (CD11b) AMAb90911 Monoclonal IHC WB 67 68
Microglia Anti-PTPRC AMAb90518 Monoclonal IHCWB 35 37
Microglia Anti-PTPRC HPA000440 Polyclonal IHCWB 35 37
Microglia Anti-TMEM119 AMAb91528 Monoclonal IHCWB ICC-IF 52 46
Microglia Anti-TMEM119 HPA051870 Polyclonal IHC 52 46
Neurons Anti-NEFM (NF160) AMAb91027 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91028 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91029 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91030 Monoclonal IHC WB 98 98
Neurons Anti-NEFH (NF200) AMAb91025 Monoclonal IHC WB 98 98
Neurons Anti-NEFH (NF200) HPA061615 Polyclonal IHC 88 94
Neurons Anti-UCHL1 AMAb91145 Monoclonal IHC WB ICC-IF 97 97
Neurons Anti-UCHL1 HPA005993 Polyclonal IHCWBICC-IF 97 97
Neurons Anti-VIM AMAb90516 Monoclonal IHCWB 99 99
Neurons Anti-VIM HPA001762 Polyclonal IHCWBICC-IF 99 99
Schwann cells Anti-MBP AMAb91062 Monoclonal IHC WB ICC-IF 97 97
Schwann cells Anti-MBP AMAb91063 Monoclonal IHC WB ICC-IF 97 97
Schwann cells Anti-MBP AMAb91064 Monoclonal IHC WB 97 97
Anti-I
TM2B antibodyNeurodegenerative diseases such as Amyotrophic Lateral Sclerosis Parkinsonrsquos Alzheimerrsquos and Huntingtonrsquos diseases occur as a result of the progressive loss of structure and function in neurons leading to the death of neurons
Age remains the primary risk factor for almost all the neurodegenerative disorders thus interfering with age-related molecular mechanisms or pathways might be an avenue to develop new therapeutics
The choline acetyltransferase (ChAT) is an enzyme responsible for catalyzing the synthesis of acetylcholine and is expressed in the majority of cholinergic neurons Hence ChAT immunoreactivity is used to detect cognitive decline in several neurodegenerative disorders
Integral membrane protein 2B (ITM2B) is a protein that in humans is encoded by the ITM2B gene This gene located on chromosome 13 is connected to familial dementia causing amyloid and pre-fibrillar effects similar to those seen in Alzheimerrsquos
The expression of the ubiquitin-carboxyl-terminal hydrolase isozyme L1 (UCHL1) present both in the neuronal cells of the central and peripheral nervous system is down-regulated in Parkinsonrsquos and Alzheimerrsquos disease patients
NEURODEGENERATIVE DISEASES
Anti-C
HAT antibody
Anti-U
CHL1 antibody
Anti-CHAT monoclonal antibody (AMAb91130) shows strong immunoreactivity in the
acetylcholine neurons in the rat diagonal band of Broca in green
Anti-ITM2B polyclonal antibody (HPA029292) targeting Integral membrane protein 2B strongly
labels Golgi apparatus in A-431 cells in green
Anti-UCHL1 monoclonal antibody (AMAb91145) in the mouse trigeminal ganglion show strong
immunoreactrivity in primary sensory neurons in green
12
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for NeurodegenerationProduct Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-ADAR AMAb90535 Monoclonal IHCWB ICC-IF 86 85
Anti-ADAR HPA003890 Polyclonal IHCWBICC-IF 86 85
Anti-AIMP1 HPA018476 Polyclonal IHCWBICC-IF 96 97
Anti-AKT1 AMAb90834 Monoclonal WB ICC-IF 97 97
Anti-AOX1 HPA040199 Polyclonal IHC 84 86
Anti-APBA3 HPA045577 Polyclonal IHC 70 68
Anti-APBB2 HPA023542 Polyclonal IHCWBICC-IF 85 83
Anti-APBB3 HPA005571 Polyclonal IHCICC-IF 84 84
Anti-APOA4 AMAb90769 Monoclonal IHC WB 65 28
Anti-APP HPA001462 Polyclonal IHCWB 95 95
Anti-AQP4 AMAb90537 Monoclonal IHCWB 93 92
Anti-AQP4 HPA014784 Polyclonal IHCWB ICC-IF 93 92
Anti-ATF2 HPA022134 Polyclonal IHCWBICC-IF 99 99
Anti-ATF3 AMAb90909 Monoclonal IHCICC-IF 92 92
Anti-ATF3 HPA001562 Polyclonal IHCICC-IF 92 92
Anti-ATRX AMAb90784 Monoclonal IHC WBICC-IF 96 97
Anti-ATXN1 HPA008335 Polyclonal IHC 81 81
Anti-ATXN2 HPA018295 Polyclonal IHCWBICC-IF 90 91
Anti-C3 HPA020432 Polyclonal IHC 78 23
Anti-CASP3 HPA002643 Polyclonal IHCWBICC-IF 84 88
Anti-CD4 AMAb90754 Monoclonal IHCWB 55 57
Anti-CD40 AMAb90905 Monoclonal IHCWB 58 54
Anti-CHAT AMAb91130 Monoclonal IHCICC-IF 96 96
Anti-CHAT HPA048547 Polyclonal IHC 96 96
Anti-CHGA AMAb90525 Monoclonal IHCWBICC-IF 62 64
Anti-COX1 HPA002834 Polyclonal IHCWBICC-IF 93 90
Anti-COX2PTGS2 HPA001335 Polyclonal IHCICC-IF 88 88
Anti-CTSB HPA018156 Polyclonal IHCICC-IF 79 79
Anti-CTSD HPA003001 Polyclonal IHCWB 86 86
Anti-CXorf27 HPA003356 Polyclonal IHC 47 46
Anti-FBXO7 (PARK15) HPA032114 Polyclonal IHC 78 81
Anti-FUS AMAb90549 Monoclonal IHC WB ICC-IF 91 91Anti-GSK3B HPA028017 Polyclonal IHCWBICC-IF 100 100Anti-HIP1 HPA017964 Polyclonal IHCWB 79 77Anti-HTRA2 (PARK13) HPA027366 Polyclonal IHCWB 57 63Anti-ITGAM (CD11b) AMAb90911 Monoclonal IHCWB 67 68
Anti-ITM2B HPA029292 Polyclonal IHC ICC-IF 95 96Anti-LRP2 HPA005980 Polyclonal IHC 78 36
Products with enhanced validation for indicated application (see page 16)
13
Product Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-NFKB1 HPA027305 Polyclonal IHCWBICC-IF 60 62
Anti-OPTN HPA003360 Polyclonal IHCWBICC-IF 64 68
Anti-PADI4 HPA017007 Polyclonal IHCWB 66 69
Anti-PARK7 HPA004190 Polyclonal IHCWB 89 90
Anti-PFN1 AMAb91181 Monoclonal IHCWB 87 87
Anti-PHGDH AMAb90786 Monoclonal IHCWBICC-IF 99 99
Anti-PHGDH HPA021241 Polyclonal IHCWBICC-IF 99 99
Anti-PRNP HPA042754 Polyclonal IHC 91 91
Anti-PSEN1 HPA030760 Polyclonal IHC 82 81
Anti-RHOT1 AMAb90852 Monoclonal IHCWB 100 100
Anti-RHOT1 HPA010687 Polyclonal IHC 100 100
Anti-S100A8 HPA024372 Polyclonal IHCWBICC-IF 56 60
Anti-SERPINA3 HPA002560 Polyclonal IHCWB 60 59
Anti-SNCB HPA035876 Polyclonal IHCWB 97 97
Anti-SOD1 HPA001401 Polyclonal IHCWBICC-IF 82 81
Anti-SOD2 HPA001814 Polyclonal IHCWB 88 87
Anti-THY1 AMAb90844 Monoclonal IHCWB 64 68
Anti-THY1 HPA003733 Polyclonal IHCICC-IF 64 68
Anti-TNFRSF21 HPA006746 Polyclonal IHCWB 86 85
Anti-TTR AMAb90649 Monoclonal IHC WB 81 83
Anti-UBE2K HPA028869 Polyclonal IHCICC-IF 100 100
Anti-UCHL1 HPA005993 Polyclonal IHCWBICC-IF 97 97
Anti-USP46 AMAb90722 Monoclonal WBICC-IF 100 99
Anti-VPS26A AMAb90967 Monoclonal IHC WBICC-IF 96 100
Anti-WHSC1 AMAb90851 Monoclonal IHCWBICC-IF 91 91
Anti-WHSC1 HPA015801 Polyclonal IHCICC-IF 91 91
Anti-VWF AMAb90928 Monoclonal IHCWB 80 80
Anti-VWF HPA002082 Polyclonal IHC 82 78
The development of the central nervous system includes the development of the brain spinal cord optic and auditory systems
Within the neural tube stem cells generate the two major classes of cells that make the majority of the nervous system neurons and glia Both these classes of cells differentiate into many different types generated with highly specialized functions and shapes
The development of the peripheral and central nervous systems begins early in embryogenesis and can be traced through the use of lineage antibodies specific to each stage of neuronal development
The (SOX) family of transcription factors are well-established regulators of cell fate decisions during development
Vimentin (VIM) is a type III intermediate filament protein expressed in mesenchymal cells It is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both healthy development and metastatic progression
The cell surface glycoprotein Thy1 (CD90) is expressed on many mammalian cell types including neurons and serves as a human mesenchymal stem cell (MSC) marker
DEVELOPMENT
A
nti-VIM antibody
A
nti-SOX4 antibody
Anti-T
HY1 antibody
Anti-VIM polyclonal antibody (HPA001762) immunofluorescent staining of human cell line
ASC TERT1 shows localization to intermediate filaments in green
Anti-SOX4 monoclonal antibody (AMAb913802) immunofluorescent staining in mouse
hippocampus showing immunoreactivity in a subset of neurons in the granule cell layer of
the dentate gyrus in brown
Anti-THY1 polyclonal antibody (HPA003733) staining of human cerebral cortex shows
localization to neuropils in brown
Product Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-ENG AMAb90925 Monoclonal IHC 66 22Anti-ENG HPA011862 Polyclonal IHCWB 66 22Anti-FABP7 AMAb90595 Monoclonal IHCWB 89 90Anti-FABP7 HPA028825 Polyclonal IHCWB 89 90Anti-FLT1 AMAb90704 Monoclonal IHCWB 80 82Anti-GAP43 HPA015600 Polyclonal IHC ICC-IF 71 70Anti-GLI3 HPA005534 Polyclonal IHC 74 76Anti-MEF2C AMAb90727 Monoclonal IHCWBICC-IF 97 47Anti-MEF2C HPA005533 Polyclonal IHCWBICC-IF 97 47Anti-MKI67 (Ki67) HPA000451 Polyclonal IHCICC-IF 66 67Anti-NACC1 HPA021238 Polyclonal IHCICC-IF 91 89Anti-NES (Nestin) AMAb90556 Monoclonal IHCWBICC-IF 47 42Anti-NES (Nestin) HPA007007 Polyclonal IHCWB 47 42Anti-NKX2-2 HPA003468 Polyclonal IHCWB 96 97Anti-PAX6 HPA030775 Polyclonal IHCICC-IF 100 100Anti-PBK HPA005753 Polyclonal IHCWBICC-IF 91 94Anti-REST AMAb90740 Monoclonal IHCICC-IF 41 43Anti-RUNX1 HPA004176 Polyclonal IHCICC-IF 93 93Anti-RUNX2 AMAb90591 Monoclonal IHCWBICC-IF 100 81Anti-RUNX2 HPA022040 Polyclonal IHCWBICC-IF 100 81Anti-SATB2 AMAb90679 Monoclonal IHCWBICC-IF 100 100Anti-SATB2 HPA029543 Polyclonal IHCWBICC-IF 100 100Anti-SOX4 HPA029901 Polyclonal IHCICC-IF 100 39Anti-SOX6 HPA001923 Polyclonal IHCICC-IF 96 96Anti-SOX7 HPA009065 Polyclonal IHCWB 91 91Anti-SOX9 AMAb90795 Monoclonal IHCWBICC-IF 97 96Anti-SOX11 AMAb90502 Monoclonal IHCWB 82 82Anti-SOX11 HPA000536 Polyclonal IHCWB 82 82Anti-SOX30 HPA006159 Polyclonal IHCWB 68 70Anti-THY1 AMAb90844 Monoclonal IHCWB 64 68Anti-THY1 HPA003733 Polyclonal IHCICC-IF 64 68Anti-TM4SF2TSPAN7 HPA003140 Polyclonal IHCWB 96 96Anti-TM4SF2TSPAN7 AMAb90621 Monoclonal IHCWB 96 96Anti-VANGL1 AMAb90600 Monoclonal WBICC-IF 95 95Anti-VIM AMAb90516 Monoclonal IHCWB 99 99Anti-VIM HPA001762 Polyclonal IHCWBICC-IF 99 99
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Development
Products with enhanced validation for indicated application (see page 16)
15
At Atlas Antibodies we extensively validate our antibodies in IHC WB and ICC-IF Enhanced Validation is performed as an additional layer of security in an application and context-specific manner Enhanced validation offers increased security of antibody specificity in a defined context This is ensured by using the most relevant validation method for each combination of protein sample and application Enhanced Validation follows the guidelines proposed by the International Working Group for Antibody Validation (IWGAV)
ENHANCED VALIDATION
GENETIC VALIDATION Antibody specificity is confirmed by downregulating the target protein on a genetic level using siRNA
ORTHOGONAL VALIDATIONThe antibody is validated by comparing the results with a non-antibody based method across multiple samples
VALIDATION BY INDEPENDENT ANTIBODIESThe antibody specificity is demonstrated by comparing two antibodies targeting different regions of the same protein
RECOMBINANT EXPRESSION VALIDATIONThe antibody binding is verified using and over-expressed or tagged version of the target protein
MIGRATION CAPTURE MS VALIDATIONThe staining pattern and the protein size detected by the antibody is compared with results obtained by a capture Mass Spectrometry (MS) method
16
Triple A PolyclonalsTM are rabbit polyclonal primary antibodies developed within the Human Protein Atlas project IHC characterization data from 44 normal and 20 cancer tissues is available on the Human Protein Atlas portal
PrecisA MonoclonalsTM are mouse monoclonal primary antibodies developed against a number of carefully selected targets Clones are selected to recognize only unique non-overlapping epitopes and isotypes
Atlas Antibodies ABVoltavaumlgen 13SE-168 69 BrommaSwedenatlasantibodiescom
Phone +46(0)8 54 59 58 50contactatlasantibodiescomorderatlasantibodiescomsupportatlasantibodiescom
Atlas Antibodies logo Triple A Polyclonals PrecisA Monoclonals PrEST Antigen and QPrEST are trademarks or registered trademarks of Atlas Antibodies AB All other trademarks are the property of their respective owners
Products are for research use only Not for use in diagnostic procedures copy Atlas Antibodies AB 2020 008_
Neu
ro M
arke
r Pan
el _
Rev
03_2
0121
5_S
fN E
ditio
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New PrecisA MonoclonalsTM for Neuroinflammation
Products with enhanced validation for indicated application (see page 16)
7
Product Name
Product Number Clonality Validated
ApplicationsAnti-AGER AMAb91634 Monoclonal IgG2a IHC
Anti-AGER AMAb91635 Monoclonal IgG1 IHC
Anti-AIF1 AMAb91671 Monoclonal IgG2b IHC
Anti-AIF1 AMAb91672 Monoclonal IgG1 IHC
Anti-AMIGO3 AMAb91677 Monoclonal IgG1 WB
Anti-ANO2 AMAb91641 Monoclonal IgG1 IHC
Anti-ANO2 AMAb91642 Monoclonal IgG1 IHC
Anti-CD163 AMAb91646 Monoclonal IgG1 IHCWB
Anti-CD163 AMAb91648 Monoclonal IgG1 IHC
Anti-CRYAB AMAb91661 Monoclonal IgG1 IHC WB ICC-IF
Anti-CRYAB AMAb91662 Monoclonal IgG2b IHC WB
Anti-CXCL13 AMAb91629 Monoclonal IgG1 IHC
Anti-DYSF AMAb91667 Monoclonal IgG1 IHC WB
Anti-GAP43 AMAb91664 Monoclonal IgG2b IHC
Anti-GAP43 AMAb91665 Monoclonal IgG1 IHC
Anti-GAP43 AMAb91666 Monoclonal IgG2a IHC
Anti-GPR17 AMAb91624 Monoclonal IgG1 IHC WB
Anti-GZMB AMAb91650 Monoclonal IgG1 IHC
Anti-HLA-DRA AMAb91673 Monoclonal IgG1 IHC WB
Anti-HLA-DRA AMAb91674 Monoclonal IgG2a IHC WB
Anti-HLA-DRA AMAb91675 Monoclonal IgG2b IHC WB
Anti-IL17A AMAb91615 Monoclonal IgG1 IHC
Anti-IL17RA AMAb91617 Monoclonal IgG2b WB
Anti-IL17RA AMAb91619 Monoclonal IgG1 WB
Anti-IL7 AMAb91684 Monoclonal IgG1 ICC-IF
Anti-ITGA4 AMAb91699 Monoclonal IgG2b WB
Anti-MS4A1 AMAb91636 Monoclonal IgG2a IHC WB
Anti-OPALIN AMAb91685 Monoclonal IgG1 IHC
Anti-OPALIN AMAb91686 Monoclonal IgG2a IHC
Anti-PLP1 AMAb91639 Monoclonal IgG1 IHC
Anti-RGMA AMAb91702 Monoclonal IgG2b WB
Anti-S100A8 AMAb91689 Monoclonal IgG1 IHC
Anti-S100A9 AMAb91690 Monoclonal IgG2a IHC
Anti-SERPINA3 AMAb91655 Monoclonal IgG1 IHC
Anti-SPP1 AMAb91653 Monoclonal IgG1 IHC
Anti-TLR2 AMAb91631 Monoclonal IgG1 WB
Anti-TRPM4 AMAb91693 Monoclonal IgG1 IHC WB ICC-IF
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Anti-AGER HPA064436 Polyclonal WB ICC-IF 41 41
Anti-AIF1 HPA049234 Polyclonal IHC 84 84
Anti-AIF1 HPA062949 Polyclonal ICC-IF 86 83
Anti-ANO2 HPA057499 Polyclonal IHC 84 85
Anti-CD163 HPA046404 Polyclonal IHC ICC-IF 51 30
Anti-CD163 HPA051974 Polyclonal IHC 75 83
Anti-CRYAB HPA057100 Polyclonal IHC 98 98
Anti-DYSF HPA017071 Polyclonal IHC ICC-IF 96 96
Anti-DYSF HPA021945 Polyclonal IHC 88 88
Anti-GAP43 HPA013392 Polyclonal IHC ICC-IF 88 86
Anti-GAP43 HPA015600 Polyclonal IHC ICC-IF 71 70
Anti-GPR17 HPA029766 Polyclonal IHC 92 92
Anti-GZMB HPA003418 Polyclonal IHC WB 63 63
Anti-HLA-DRA HPA050162 Polyclonal IHC 54 69
Anti-HLA-DRA HPA053176 Polyclonal IHC 41 83
Anti-IL7 HPA019590 Polyclonal IHC 46 46
Anti-ITGA4 HPA074961 Polyclonal ICC-IF 90 89
Anti-MS4A1 HPA014341 Polyclonal IHC WB ICC-IF 73 77
Anti-MS4A1 HPA014391 Polyclonal IHC WB 61 64
Anti-OPALIN HPA014372 Polyclonal IHC 72 75
Anti-PLP1 HPA004128 Polyclonal IHC 100 100
Anti-RGMA HPA039880 Polyclonal ICC-IF 96 96
Anti-RGMA HPA044222 Polyclonal ICC-IF 87 87
Anti-S100A8 HPA024372 Polyclonal IHC WB ICC-IF 56 60
Anti-SERPINA3 HPA000893 Polyclonal IHC WB 61 61
Anti-SERPINA3 HPA002560 Polyclonal IHC WB 60 59
Anti-SPP1 HPA027541 Polyclonal IHC WB ICC-IF 56 53
Anti-TRPM4 HPA041169 Polyclonal IHC ICC-IF 93 91
NEW PRODUCTS
Selected Triple A PolyclonalsTM for Neuroinflammation
Products with enhanced validation for indicated application (see page 16)
Synaptic signaling is cell-cell communication that occurs at the synaptic level Cells communicate with each other via direct contact (juxtacrine signaling) over short distances (paracrine signaling) or vast distances and scales (endocrine signaling) Other cells form gap junctions that connect their cytoplasm to the cytoplasm of adjacent cells
When the sending neuron fires an electrical impulse travels along the axon As soon as the impulse reaches the synapse it triggers the release of neurotransmitters
The predominant excitatory neurotransmitter in the brain is glutamate (GLU) The inhibitory neurotransmitters GABA (in the brain) and glycine (in the brainstem and spinal cord) are common amino acids that participate in multiple metabolic cycles
Calcium (Ca2+) signaling in neurons is critical in cell signalingIt defines the ability of cells to release neurotransmitters thus regulating the intracellular signaling pathways Calbindin 2 (also known as calretinin) is one of the Ca2+-binding proteins involved in Ca2+ signaling that facilitates cytosolic Ca2+ diffusion without interfering with Ca2+ signaling
SYNAPTIC SIGNALING
A
nti-CALB2 antibody
An
ti-mGluR1 antibody
A
nti-VGAT antibody
Anti-VGAT monoclonal antibody (AMAb91043) shows strong immunoreactivity in GABAergic
fibers in the rat globus pallidus in green
Anti-CALB2 polyclonal antibody (HPA007305) shows the labeling in green of mouse
hippocampus Note the strong labeling in the dentate gyrus
Anti-MGLUR1 polyclonal antibody (HPA015701) strongly labels cortical perikarya in human
cerebral cortex tissue in brown
8
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Synaptic Signaling
9
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Anti-ATF2 HPA022134 Polyclonal IHC WB ICC-IF 99 99
Anti-ATF3 AMAb90909 Monoclonal IHC ICC-IF 92 92
Anti-ATF3 HPA001562 Polyclonal IHC ICC-IF 92 92
Anti-ATP1B1 HPA012911 Polyclonal IHC WB 93 93
Anti-ATP1B2 HPA010698 Polyclonal IHC 96 88
Anti-CAMK2B HPA026307 Polyclonal IHC WB 96 96
Anti-CALB1 HPA023099 Polyclonal IHC WB ICC-IF 98 99
Anti-CALB2 HPA007306 Polyclonal IHC WB ICC-IF 100 100
Anti-CALB2 HPA007305 Polyclonal IHC WB ICC-IF 100 100
Anti-CAMK2D HPA026281 Polyclonal IHC 100 97
Anti-C-FOS HPA018531 Polyclonal IHC ICC-IF 94 94
Anti-CHAT AMAb91130 Monoclonal IHC ICC-IF 96 96
Anti-CHAT HPA048547 Polyclonal IHC 96 96
Anti-CHRM1 (M1 mAChR) HPA014101 Polyclonal IHC 98 97
Anti-CHRM2 (M2 AChR) HPA029795 Polyclonal IHC 88 86
Anti-CLIC4 HPA008019 Polyclonal IHC WB 98 97
Anti-CREB1 HPA019150 Polyclonal IHC WB ICC-IF 100 100
Anti-DDC AMAb91089 Monoclonal IHCWB 90 88
Anti-DDC HPA017742 Polyclonal IHCWBICC-IF 90 88
Anti-EAAC1 HPA020086 Polyclonal IHC WB 77 79
Anti-EAAT2 HPA009172 Polyclonal IHC 87 89
Anti-GABRA3 HPA000839 Polyclonal IHCWB 91 93
Anti-GABRB1 HPA051297 Polyclonal IHC 97 100
Anti-GABRG1 HPA035622 Polyclonal IHC 96 94
Anti-GAD1 (GAD67) AMAb91076 Monoclonal IHCWB 94 94
Anti-GAD1 HPA058412 Polyclonal IHCWB 94 94
Anti-GAD2 AMAb91048 Monoclonal IHCWB 84 88
Anti-GAD2 HPA044637 Polyclonal IHC 84 88
Anti-GAT1 HPA013341 Polyclonal IHC WB 98 98
Anti-GAT3 HPA037981 Polyclonal IHCWB 85 87
Anti-GLUR2 (AMPA2) HPA008441 Polyclonal IHC 100 100
Anti-HTR2A HPA014011 Polyclonal IHC 95 97
Anti-KCC4 HPA041652 Polyclonal IHCWB 84 82
Anti-KCNJ5 (KIR34) HPA017353 Polyclonal IHCWB 89 89
Anti-KIF11 HPA010568 Polyclonal IHCWB 88 83
Anti-KIF17 HPA032085 Polyclonal IHCICC-IF 85 82
Anti-KIF18A HPA039312 Polyclonal IHCWBICC-IF 80 82
Anti-KIF1C HPA024602 Polyclonal IHCWB 81 83
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Anti-KIF26B HPA028562 Polyclonal IHCICC-IF 88 80
Anti-KIF4A (KIF4A amp B) HPA034745 Polyclonal IHCWBICC-IF 64 63
Anti-KIF5A HPA004469 Polyclonal IHCWB 91 88
Anti-KIF5C HPA035210 Polyclonal IHCWB 100100
Anti-KIF7 HPA043145 Polyclonal IHC 69 69
Anti-KIFAP3 HPA023742 Polyclonal IHC 100 100
Anti-MAPK1 (ERK) HPA030069 Polyclonal IHCWB 100 100
Anti-MAPK3 (ERK1) HPA005700 Polyclonal IHCWB 98 98
Anti-MGLUR1 HPA015701 Polyclonal IHC 80 80
Anti-MGLUR8 HPA051481 Polyclonal IHC 95 90
Anti-NCS1 HPA019713 Polyclonal IHCWBICC-IF 100 100
Anti-PRKCA HPA006563 Polyclonal IHCWBICC-IF 99 99
Anti-PRKCH HPA053709 Polyclonal IHCICC-IF 97 64
Anti-PNMT HPA051005 Polyclonal IHC 89 92
Anti-PRKACB (PKACB) HPA029754 Polyclonal IHC 73 76
Anti-PRKCZ HPA021851 Polyclonal IHCWB ICC-IF 94 94
Anti-RAB3A HPA003160 Polyclonal IHC 99 99
Anti-RAP1GAP HPA001922 Polyclonal IHC 92 91
Anti-SLC17A7 (VGLUT1) AMAb91041 Monoclonal IHCWB 94 94
Anti-SLC22A2 HPA008567 Polyclonal IHC 84 77
Anti-SLC32A1 (VGAT) HPA058859 Polyclonal IHC 95 93
Anti-SLC6A2 (NET) AMAb91116 Monoclonal IHC -
Anti-SLC6A3 (DAT) AMAb91125 Monoclonal IHC 85 85
Anti-SNAP25 HPA001830 Polyclonal IHCWB 100 100
Anti-SNAP29 HPA031823 Polyclonal IHC 89 92
Anti-SST (SOM) HPA019472 Polyclonal IHCWB 98 98
Anti-STXBP1 HPA008209 Polyclonal IHCWBICC-IF 100 100
Anti-STXBP6 HPA003552 Polyclonal IHCWB 99 99
Anti-SYNGR1 HPA029673 Polyclonal IHC ICC-IF 90 88
Anti-SYNPR HPA061671 Polyclonal IHC 97 97
Anti-SYP HPA002858 Polyclonal IHCWB 83 83
Anti-SYT1 HPA008394 Polyclonal IHCWB 100 100
Anti-SYT12 HPA011006 Polyclonal IHC 96 98
Anti-SYT13 HPA046224 Polyclonal IHC 96 93
Anti-SYT16 HPA004199 Polyclonal IHCWB 95 95
Anti-TGFA HPA042297 Polyclonal IHC 93 93
Anti-TH AMAb91112 Monoclonal IHC 88 88
Anti-TH HPA061003 Polyclonal IHC WB 88 88
Anti-TPH2 (NTPH) AMAb91108 Monoclonal IHC 100 100
Anti-VAMP4 HPA050418 Polyclonal IHCICC-IF 100 100
Anti-VAMP7 HPA036733 Polyclonal IHCICC-IF 98 93
Products with enhanced validation for indicated application (see page 16)
NEURAL LINEAGE
A
nti-GFAP antibody
Anti-C
NP antibody
Anti-N
EFM antibodyDuring central nervous system development neural stem cells (NSCs) proliferate and divide to generate a progeny that differentiate into neurons astrocytes oligodendrocytes and different glial cells
NSCs self-renewal and differentiation are regulated by a precise temporal sequence of growth factors intracellular signaling and transcription factor expression NSCs and their differentiated progeny can be identified by the expression of a unique combination of cell surface markers and transcription factors
The glial fibrillary acidic protein (GFAP) is an intermediate filament (IF) protein and a well-known marker for astrocytes
Oligodendrocytes can convert extracellular 2rsquo3rsquo-cAMP to adenosine As CNPase is the predominant enzyme involved in this conversion the cyclic nucleotide phosphodiesterase (CNP) is widely used as an oligodendrocyte marker
Neurons express the neurofilament medium (NFEM) polypeptide other intermediate filament proteins including nestin synemin syncoilin and vimentin
Anti-GFAP polyclonal antibody (HPA056030) shows strong immunoreactivity in the
human cerebellum in brown
Anti-NEFM polyclonal antibody (HPA022845) shows positivity in a subset of neuronal cells in
human cerebral cortex in brown
Anti-CNP polyclonal antibody (HPA023266) in the rat cerebral cortex is shown in magenta
10
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Neural Lineage
Products with enhanced validation for indicated application (see page 16)
11
TargetCells
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Astrocytes Anti-GFAP AMAb91033 Monoclonal IHC WB 98 100
Astrocytes Anti-GFAP HPA056030 Polyclonal IHCWB ICC-IF 98 100
Astrocytes Anti-GLUL AMAb91101 Monoclonal IHC WB 95 53
Astrocytes Anti-GLUL AMAb91102 Monoclonal IHC WB 95 92
Astrocytes Anti-GLUL AMAb91103 Monoclonal IHC WB 95 92
Astrocytes Anti-GLUL HPA007316 Polyclonal IHCWB 95 53
Astrocytes Anti-S100B AMAb91038 Monoclonal IHC WB 99 98
Astroglia Anti-EZR AMAb90976 Monoclonal IHCWBICC-IF 93 93
Astroglia Anti-EZR HPA021616 Polyclonal IHCWBICC-IF 93 93
Adhesion molecule Anti-ICAM5 HPA009083 Polyclonal IHC 85 86
Adhesion molecule Anti-MCAM HPA008848 Polyclonal IHC WB 75 73
Adhesion molecule Anti-NCAM2 HPA030900 Polyclonal IHC 89 91
Adhesion molecule Anti-NLGN1 HPA006680 Polyclonal IHCWB 98 98
Calcium binding protein Anti-CALB1 (CB) HPA023099 Polyclonal IHCWBICC-IF 98 99
Calcium binding protein Anti-CALB2 (CR) HPA007305 Polyclonal IHCWBICC-IF 100 100
Calcium binding proteins Anti-NECAB1 AMAb90801 Monoclonal IHCWB 98 98
Calcium binding protein Anti-NECAB1 HPA023629 Polyclonal IHCWB 98 98
Calcium binding protein Anti-NECAB2 AMAb90808 Monoclonal IHC 85 84
Calcium binding protein Anti-NECAB2 HPA013998 Polyclonal IHCICC-IF 98 97
Calcium binding protein Anti-SCGN AMAb90630 Monoclonal IHCWB 96 96
Calcium binding protein Anti-SCGN HPA006641 Polyclonal IHC 96 96
Calcium binding protein Anti-S100B HPA015768 Polyclonal IHCWBICC-IF 99 98
Oligodendrocytes Anti-MOG AMAb91066 Monoclonal IHC WB 91 89
Oligodendrocytes Anti-MOG AMAb91067 Monoclonal IHC WB 91 89
Oligodendrocytes Anti-MOG HPA021873 Polyclonal IHC 91 89
Oligodendrocytes Anti-CNP AMAb91068 Monoclonal IHC WB 76 77
Oligodendrocytes Anti-CNP AMAb91069 Monoclonal IHC WB 76 77
Oligodendrocytes Anti-CNP AMAb91072 Monoclonal IHC WB ICC-IF 76 77
Oligodendrocytes Anti-CNP HPA023280 Polyclonal IHCWBICC-IF 76 77
Progenitors Anti-PBK HPA005753 Polyclonal IHCWBICC-IF 91 94
Progenitors Anti-MKI67 (Ki67) AMAb90870 Monoclonal IHC ICC-IF 68 68
Progenitors Anti-MKI67 (Ki67) HPA000451 Polyclonal IHCICC-IF 66 67
TargetCells
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Cytoskeleton Anti-ACTN1 HPA006035 Polyclonal IHCWB 98 99
Cytoskeleton Anti-ACTN4 HPA001873 Polyclonal IHCWBICC-IF 99 98
Cytoskeleton Anti-INA HPA008057 Polyclonal IHC WB ICC-IF 83 84
Cytoskeleton Anti-MAP1A HPA039064 Polyclonal IHC 60 52
Cytoskeleton Anti-MAP1B HPA022275 Polyclonal IHCWB ICC-IF 85 86
Cytoskeleton Anti-MAP2 HPA008273 Polyclonal IHC WB ICC-IF 96 96
Cytoskeleton Anti-MYO5A HPA001356 Polyclonal IHCICC-IF 99 98
Cytoskeleton Anti-NEFM (NF160) HPA022845 Polyclonal IHCWBICC-IF 98 98
Microglia Anti-AIF HPA049234 Polyclonal IHC 84 84
Microglia Anti-CD68 AMAb90874 Monoclonal IHCWB 76 76
Microglia Anti-CD68 HPA048982 Polyclonal IHC WB 76 76
Microglia Anti-ITGAM (CD11b) AMAb90911 Monoclonal IHC WB 67 68
Microglia Anti-PTPRC AMAb90518 Monoclonal IHCWB 35 37
Microglia Anti-PTPRC HPA000440 Polyclonal IHCWB 35 37
Microglia Anti-TMEM119 AMAb91528 Monoclonal IHCWB ICC-IF 52 46
Microglia Anti-TMEM119 HPA051870 Polyclonal IHC 52 46
Neurons Anti-NEFM (NF160) AMAb91027 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91028 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91029 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91030 Monoclonal IHC WB 98 98
Neurons Anti-NEFH (NF200) AMAb91025 Monoclonal IHC WB 98 98
Neurons Anti-NEFH (NF200) HPA061615 Polyclonal IHC 88 94
Neurons Anti-UCHL1 AMAb91145 Monoclonal IHC WB ICC-IF 97 97
Neurons Anti-UCHL1 HPA005993 Polyclonal IHCWBICC-IF 97 97
Neurons Anti-VIM AMAb90516 Monoclonal IHCWB 99 99
Neurons Anti-VIM HPA001762 Polyclonal IHCWBICC-IF 99 99
Schwann cells Anti-MBP AMAb91062 Monoclonal IHC WB ICC-IF 97 97
Schwann cells Anti-MBP AMAb91063 Monoclonal IHC WB ICC-IF 97 97
Schwann cells Anti-MBP AMAb91064 Monoclonal IHC WB 97 97
Anti-I
TM2B antibodyNeurodegenerative diseases such as Amyotrophic Lateral Sclerosis Parkinsonrsquos Alzheimerrsquos and Huntingtonrsquos diseases occur as a result of the progressive loss of structure and function in neurons leading to the death of neurons
Age remains the primary risk factor for almost all the neurodegenerative disorders thus interfering with age-related molecular mechanisms or pathways might be an avenue to develop new therapeutics
The choline acetyltransferase (ChAT) is an enzyme responsible for catalyzing the synthesis of acetylcholine and is expressed in the majority of cholinergic neurons Hence ChAT immunoreactivity is used to detect cognitive decline in several neurodegenerative disorders
Integral membrane protein 2B (ITM2B) is a protein that in humans is encoded by the ITM2B gene This gene located on chromosome 13 is connected to familial dementia causing amyloid and pre-fibrillar effects similar to those seen in Alzheimerrsquos
The expression of the ubiquitin-carboxyl-terminal hydrolase isozyme L1 (UCHL1) present both in the neuronal cells of the central and peripheral nervous system is down-regulated in Parkinsonrsquos and Alzheimerrsquos disease patients
NEURODEGENERATIVE DISEASES
Anti-C
HAT antibody
Anti-U
CHL1 antibody
Anti-CHAT monoclonal antibody (AMAb91130) shows strong immunoreactivity in the
acetylcholine neurons in the rat diagonal band of Broca in green
Anti-ITM2B polyclonal antibody (HPA029292) targeting Integral membrane protein 2B strongly
labels Golgi apparatus in A-431 cells in green
Anti-UCHL1 monoclonal antibody (AMAb91145) in the mouse trigeminal ganglion show strong
immunoreactrivity in primary sensory neurons in green
12
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for NeurodegenerationProduct Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-ADAR AMAb90535 Monoclonal IHCWB ICC-IF 86 85
Anti-ADAR HPA003890 Polyclonal IHCWBICC-IF 86 85
Anti-AIMP1 HPA018476 Polyclonal IHCWBICC-IF 96 97
Anti-AKT1 AMAb90834 Monoclonal WB ICC-IF 97 97
Anti-AOX1 HPA040199 Polyclonal IHC 84 86
Anti-APBA3 HPA045577 Polyclonal IHC 70 68
Anti-APBB2 HPA023542 Polyclonal IHCWBICC-IF 85 83
Anti-APBB3 HPA005571 Polyclonal IHCICC-IF 84 84
Anti-APOA4 AMAb90769 Monoclonal IHC WB 65 28
Anti-APP HPA001462 Polyclonal IHCWB 95 95
Anti-AQP4 AMAb90537 Monoclonal IHCWB 93 92
Anti-AQP4 HPA014784 Polyclonal IHCWB ICC-IF 93 92
Anti-ATF2 HPA022134 Polyclonal IHCWBICC-IF 99 99
Anti-ATF3 AMAb90909 Monoclonal IHCICC-IF 92 92
Anti-ATF3 HPA001562 Polyclonal IHCICC-IF 92 92
Anti-ATRX AMAb90784 Monoclonal IHC WBICC-IF 96 97
Anti-ATXN1 HPA008335 Polyclonal IHC 81 81
Anti-ATXN2 HPA018295 Polyclonal IHCWBICC-IF 90 91
Anti-C3 HPA020432 Polyclonal IHC 78 23
Anti-CASP3 HPA002643 Polyclonal IHCWBICC-IF 84 88
Anti-CD4 AMAb90754 Monoclonal IHCWB 55 57
Anti-CD40 AMAb90905 Monoclonal IHCWB 58 54
Anti-CHAT AMAb91130 Monoclonal IHCICC-IF 96 96
Anti-CHAT HPA048547 Polyclonal IHC 96 96
Anti-CHGA AMAb90525 Monoclonal IHCWBICC-IF 62 64
Anti-COX1 HPA002834 Polyclonal IHCWBICC-IF 93 90
Anti-COX2PTGS2 HPA001335 Polyclonal IHCICC-IF 88 88
Anti-CTSB HPA018156 Polyclonal IHCICC-IF 79 79
Anti-CTSD HPA003001 Polyclonal IHCWB 86 86
Anti-CXorf27 HPA003356 Polyclonal IHC 47 46
Anti-FBXO7 (PARK15) HPA032114 Polyclonal IHC 78 81
Anti-FUS AMAb90549 Monoclonal IHC WB ICC-IF 91 91Anti-GSK3B HPA028017 Polyclonal IHCWBICC-IF 100 100Anti-HIP1 HPA017964 Polyclonal IHCWB 79 77Anti-HTRA2 (PARK13) HPA027366 Polyclonal IHCWB 57 63Anti-ITGAM (CD11b) AMAb90911 Monoclonal IHCWB 67 68
Anti-ITM2B HPA029292 Polyclonal IHC ICC-IF 95 96Anti-LRP2 HPA005980 Polyclonal IHC 78 36
Products with enhanced validation for indicated application (see page 16)
13
Product Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-NFKB1 HPA027305 Polyclonal IHCWBICC-IF 60 62
Anti-OPTN HPA003360 Polyclonal IHCWBICC-IF 64 68
Anti-PADI4 HPA017007 Polyclonal IHCWB 66 69
Anti-PARK7 HPA004190 Polyclonal IHCWB 89 90
Anti-PFN1 AMAb91181 Monoclonal IHCWB 87 87
Anti-PHGDH AMAb90786 Monoclonal IHCWBICC-IF 99 99
Anti-PHGDH HPA021241 Polyclonal IHCWBICC-IF 99 99
Anti-PRNP HPA042754 Polyclonal IHC 91 91
Anti-PSEN1 HPA030760 Polyclonal IHC 82 81
Anti-RHOT1 AMAb90852 Monoclonal IHCWB 100 100
Anti-RHOT1 HPA010687 Polyclonal IHC 100 100
Anti-S100A8 HPA024372 Polyclonal IHCWBICC-IF 56 60
Anti-SERPINA3 HPA002560 Polyclonal IHCWB 60 59
Anti-SNCB HPA035876 Polyclonal IHCWB 97 97
Anti-SOD1 HPA001401 Polyclonal IHCWBICC-IF 82 81
Anti-SOD2 HPA001814 Polyclonal IHCWB 88 87
Anti-THY1 AMAb90844 Monoclonal IHCWB 64 68
Anti-THY1 HPA003733 Polyclonal IHCICC-IF 64 68
Anti-TNFRSF21 HPA006746 Polyclonal IHCWB 86 85
Anti-TTR AMAb90649 Monoclonal IHC WB 81 83
Anti-UBE2K HPA028869 Polyclonal IHCICC-IF 100 100
Anti-UCHL1 HPA005993 Polyclonal IHCWBICC-IF 97 97
Anti-USP46 AMAb90722 Monoclonal WBICC-IF 100 99
Anti-VPS26A AMAb90967 Monoclonal IHC WBICC-IF 96 100
Anti-WHSC1 AMAb90851 Monoclonal IHCWBICC-IF 91 91
Anti-WHSC1 HPA015801 Polyclonal IHCICC-IF 91 91
Anti-VWF AMAb90928 Monoclonal IHCWB 80 80
Anti-VWF HPA002082 Polyclonal IHC 82 78
The development of the central nervous system includes the development of the brain spinal cord optic and auditory systems
Within the neural tube stem cells generate the two major classes of cells that make the majority of the nervous system neurons and glia Both these classes of cells differentiate into many different types generated with highly specialized functions and shapes
The development of the peripheral and central nervous systems begins early in embryogenesis and can be traced through the use of lineage antibodies specific to each stage of neuronal development
The (SOX) family of transcription factors are well-established regulators of cell fate decisions during development
Vimentin (VIM) is a type III intermediate filament protein expressed in mesenchymal cells It is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both healthy development and metastatic progression
The cell surface glycoprotein Thy1 (CD90) is expressed on many mammalian cell types including neurons and serves as a human mesenchymal stem cell (MSC) marker
DEVELOPMENT
A
nti-VIM antibody
A
nti-SOX4 antibody
Anti-T
HY1 antibody
Anti-VIM polyclonal antibody (HPA001762) immunofluorescent staining of human cell line
ASC TERT1 shows localization to intermediate filaments in green
Anti-SOX4 monoclonal antibody (AMAb913802) immunofluorescent staining in mouse
hippocampus showing immunoreactivity in a subset of neurons in the granule cell layer of
the dentate gyrus in brown
Anti-THY1 polyclonal antibody (HPA003733) staining of human cerebral cortex shows
localization to neuropils in brown
Product Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-ENG AMAb90925 Monoclonal IHC 66 22Anti-ENG HPA011862 Polyclonal IHCWB 66 22Anti-FABP7 AMAb90595 Monoclonal IHCWB 89 90Anti-FABP7 HPA028825 Polyclonal IHCWB 89 90Anti-FLT1 AMAb90704 Monoclonal IHCWB 80 82Anti-GAP43 HPA015600 Polyclonal IHC ICC-IF 71 70Anti-GLI3 HPA005534 Polyclonal IHC 74 76Anti-MEF2C AMAb90727 Monoclonal IHCWBICC-IF 97 47Anti-MEF2C HPA005533 Polyclonal IHCWBICC-IF 97 47Anti-MKI67 (Ki67) HPA000451 Polyclonal IHCICC-IF 66 67Anti-NACC1 HPA021238 Polyclonal IHCICC-IF 91 89Anti-NES (Nestin) AMAb90556 Monoclonal IHCWBICC-IF 47 42Anti-NES (Nestin) HPA007007 Polyclonal IHCWB 47 42Anti-NKX2-2 HPA003468 Polyclonal IHCWB 96 97Anti-PAX6 HPA030775 Polyclonal IHCICC-IF 100 100Anti-PBK HPA005753 Polyclonal IHCWBICC-IF 91 94Anti-REST AMAb90740 Monoclonal IHCICC-IF 41 43Anti-RUNX1 HPA004176 Polyclonal IHCICC-IF 93 93Anti-RUNX2 AMAb90591 Monoclonal IHCWBICC-IF 100 81Anti-RUNX2 HPA022040 Polyclonal IHCWBICC-IF 100 81Anti-SATB2 AMAb90679 Monoclonal IHCWBICC-IF 100 100Anti-SATB2 HPA029543 Polyclonal IHCWBICC-IF 100 100Anti-SOX4 HPA029901 Polyclonal IHCICC-IF 100 39Anti-SOX6 HPA001923 Polyclonal IHCICC-IF 96 96Anti-SOX7 HPA009065 Polyclonal IHCWB 91 91Anti-SOX9 AMAb90795 Monoclonal IHCWBICC-IF 97 96Anti-SOX11 AMAb90502 Monoclonal IHCWB 82 82Anti-SOX11 HPA000536 Polyclonal IHCWB 82 82Anti-SOX30 HPA006159 Polyclonal IHCWB 68 70Anti-THY1 AMAb90844 Monoclonal IHCWB 64 68Anti-THY1 HPA003733 Polyclonal IHCICC-IF 64 68Anti-TM4SF2TSPAN7 HPA003140 Polyclonal IHCWB 96 96Anti-TM4SF2TSPAN7 AMAb90621 Monoclonal IHCWB 96 96Anti-VANGL1 AMAb90600 Monoclonal WBICC-IF 95 95Anti-VIM AMAb90516 Monoclonal IHCWB 99 99Anti-VIM HPA001762 Polyclonal IHCWBICC-IF 99 99
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Development
Products with enhanced validation for indicated application (see page 16)
15
At Atlas Antibodies we extensively validate our antibodies in IHC WB and ICC-IF Enhanced Validation is performed as an additional layer of security in an application and context-specific manner Enhanced validation offers increased security of antibody specificity in a defined context This is ensured by using the most relevant validation method for each combination of protein sample and application Enhanced Validation follows the guidelines proposed by the International Working Group for Antibody Validation (IWGAV)
ENHANCED VALIDATION
GENETIC VALIDATION Antibody specificity is confirmed by downregulating the target protein on a genetic level using siRNA
ORTHOGONAL VALIDATIONThe antibody is validated by comparing the results with a non-antibody based method across multiple samples
VALIDATION BY INDEPENDENT ANTIBODIESThe antibody specificity is demonstrated by comparing two antibodies targeting different regions of the same protein
RECOMBINANT EXPRESSION VALIDATIONThe antibody binding is verified using and over-expressed or tagged version of the target protein
MIGRATION CAPTURE MS VALIDATIONThe staining pattern and the protein size detected by the antibody is compared with results obtained by a capture Mass Spectrometry (MS) method
16
Triple A PolyclonalsTM are rabbit polyclonal primary antibodies developed within the Human Protein Atlas project IHC characterization data from 44 normal and 20 cancer tissues is available on the Human Protein Atlas portal
PrecisA MonoclonalsTM are mouse monoclonal primary antibodies developed against a number of carefully selected targets Clones are selected to recognize only unique non-overlapping epitopes and isotypes
Atlas Antibodies ABVoltavaumlgen 13SE-168 69 BrommaSwedenatlasantibodiescom
Phone +46(0)8 54 59 58 50contactatlasantibodiescomorderatlasantibodiescomsupportatlasantibodiescom
Atlas Antibodies logo Triple A Polyclonals PrecisA Monoclonals PrEST Antigen and QPrEST are trademarks or registered trademarks of Atlas Antibodies AB All other trademarks are the property of their respective owners
Products are for research use only Not for use in diagnostic procedures copy Atlas Antibodies AB 2020 008_
Neu
ro M
arke
r Pan
el _
Rev
03_2
0121
5_S
fN E
ditio
n
Synaptic signaling is cell-cell communication that occurs at the synaptic level Cells communicate with each other via direct contact (juxtacrine signaling) over short distances (paracrine signaling) or vast distances and scales (endocrine signaling) Other cells form gap junctions that connect their cytoplasm to the cytoplasm of adjacent cells
When the sending neuron fires an electrical impulse travels along the axon As soon as the impulse reaches the synapse it triggers the release of neurotransmitters
The predominant excitatory neurotransmitter in the brain is glutamate (GLU) The inhibitory neurotransmitters GABA (in the brain) and glycine (in the brainstem and spinal cord) are common amino acids that participate in multiple metabolic cycles
Calcium (Ca2+) signaling in neurons is critical in cell signalingIt defines the ability of cells to release neurotransmitters thus regulating the intracellular signaling pathways Calbindin 2 (also known as calretinin) is one of the Ca2+-binding proteins involved in Ca2+ signaling that facilitates cytosolic Ca2+ diffusion without interfering with Ca2+ signaling
SYNAPTIC SIGNALING
A
nti-CALB2 antibody
An
ti-mGluR1 antibody
A
nti-VGAT antibody
Anti-VGAT monoclonal antibody (AMAb91043) shows strong immunoreactivity in GABAergic
fibers in the rat globus pallidus in green
Anti-CALB2 polyclonal antibody (HPA007305) shows the labeling in green of mouse
hippocampus Note the strong labeling in the dentate gyrus
Anti-MGLUR1 polyclonal antibody (HPA015701) strongly labels cortical perikarya in human
cerebral cortex tissue in brown
8
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Synaptic Signaling
9
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Anti-ATF2 HPA022134 Polyclonal IHC WB ICC-IF 99 99
Anti-ATF3 AMAb90909 Monoclonal IHC ICC-IF 92 92
Anti-ATF3 HPA001562 Polyclonal IHC ICC-IF 92 92
Anti-ATP1B1 HPA012911 Polyclonal IHC WB 93 93
Anti-ATP1B2 HPA010698 Polyclonal IHC 96 88
Anti-CAMK2B HPA026307 Polyclonal IHC WB 96 96
Anti-CALB1 HPA023099 Polyclonal IHC WB ICC-IF 98 99
Anti-CALB2 HPA007306 Polyclonal IHC WB ICC-IF 100 100
Anti-CALB2 HPA007305 Polyclonal IHC WB ICC-IF 100 100
Anti-CAMK2D HPA026281 Polyclonal IHC 100 97
Anti-C-FOS HPA018531 Polyclonal IHC ICC-IF 94 94
Anti-CHAT AMAb91130 Monoclonal IHC ICC-IF 96 96
Anti-CHAT HPA048547 Polyclonal IHC 96 96
Anti-CHRM1 (M1 mAChR) HPA014101 Polyclonal IHC 98 97
Anti-CHRM2 (M2 AChR) HPA029795 Polyclonal IHC 88 86
Anti-CLIC4 HPA008019 Polyclonal IHC WB 98 97
Anti-CREB1 HPA019150 Polyclonal IHC WB ICC-IF 100 100
Anti-DDC AMAb91089 Monoclonal IHCWB 90 88
Anti-DDC HPA017742 Polyclonal IHCWBICC-IF 90 88
Anti-EAAC1 HPA020086 Polyclonal IHC WB 77 79
Anti-EAAT2 HPA009172 Polyclonal IHC 87 89
Anti-GABRA3 HPA000839 Polyclonal IHCWB 91 93
Anti-GABRB1 HPA051297 Polyclonal IHC 97 100
Anti-GABRG1 HPA035622 Polyclonal IHC 96 94
Anti-GAD1 (GAD67) AMAb91076 Monoclonal IHCWB 94 94
Anti-GAD1 HPA058412 Polyclonal IHCWB 94 94
Anti-GAD2 AMAb91048 Monoclonal IHCWB 84 88
Anti-GAD2 HPA044637 Polyclonal IHC 84 88
Anti-GAT1 HPA013341 Polyclonal IHC WB 98 98
Anti-GAT3 HPA037981 Polyclonal IHCWB 85 87
Anti-GLUR2 (AMPA2) HPA008441 Polyclonal IHC 100 100
Anti-HTR2A HPA014011 Polyclonal IHC 95 97
Anti-KCC4 HPA041652 Polyclonal IHCWB 84 82
Anti-KCNJ5 (KIR34) HPA017353 Polyclonal IHCWB 89 89
Anti-KIF11 HPA010568 Polyclonal IHCWB 88 83
Anti-KIF17 HPA032085 Polyclonal IHCICC-IF 85 82
Anti-KIF18A HPA039312 Polyclonal IHCWBICC-IF 80 82
Anti-KIF1C HPA024602 Polyclonal IHCWB 81 83
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Anti-KIF26B HPA028562 Polyclonal IHCICC-IF 88 80
Anti-KIF4A (KIF4A amp B) HPA034745 Polyclonal IHCWBICC-IF 64 63
Anti-KIF5A HPA004469 Polyclonal IHCWB 91 88
Anti-KIF5C HPA035210 Polyclonal IHCWB 100100
Anti-KIF7 HPA043145 Polyclonal IHC 69 69
Anti-KIFAP3 HPA023742 Polyclonal IHC 100 100
Anti-MAPK1 (ERK) HPA030069 Polyclonal IHCWB 100 100
Anti-MAPK3 (ERK1) HPA005700 Polyclonal IHCWB 98 98
Anti-MGLUR1 HPA015701 Polyclonal IHC 80 80
Anti-MGLUR8 HPA051481 Polyclonal IHC 95 90
Anti-NCS1 HPA019713 Polyclonal IHCWBICC-IF 100 100
Anti-PRKCA HPA006563 Polyclonal IHCWBICC-IF 99 99
Anti-PRKCH HPA053709 Polyclonal IHCICC-IF 97 64
Anti-PNMT HPA051005 Polyclonal IHC 89 92
Anti-PRKACB (PKACB) HPA029754 Polyclonal IHC 73 76
Anti-PRKCZ HPA021851 Polyclonal IHCWB ICC-IF 94 94
Anti-RAB3A HPA003160 Polyclonal IHC 99 99
Anti-RAP1GAP HPA001922 Polyclonal IHC 92 91
Anti-SLC17A7 (VGLUT1) AMAb91041 Monoclonal IHCWB 94 94
Anti-SLC22A2 HPA008567 Polyclonal IHC 84 77
Anti-SLC32A1 (VGAT) HPA058859 Polyclonal IHC 95 93
Anti-SLC6A2 (NET) AMAb91116 Monoclonal IHC -
Anti-SLC6A3 (DAT) AMAb91125 Monoclonal IHC 85 85
Anti-SNAP25 HPA001830 Polyclonal IHCWB 100 100
Anti-SNAP29 HPA031823 Polyclonal IHC 89 92
Anti-SST (SOM) HPA019472 Polyclonal IHCWB 98 98
Anti-STXBP1 HPA008209 Polyclonal IHCWBICC-IF 100 100
Anti-STXBP6 HPA003552 Polyclonal IHCWB 99 99
Anti-SYNGR1 HPA029673 Polyclonal IHC ICC-IF 90 88
Anti-SYNPR HPA061671 Polyclonal IHC 97 97
Anti-SYP HPA002858 Polyclonal IHCWB 83 83
Anti-SYT1 HPA008394 Polyclonal IHCWB 100 100
Anti-SYT12 HPA011006 Polyclonal IHC 96 98
Anti-SYT13 HPA046224 Polyclonal IHC 96 93
Anti-SYT16 HPA004199 Polyclonal IHCWB 95 95
Anti-TGFA HPA042297 Polyclonal IHC 93 93
Anti-TH AMAb91112 Monoclonal IHC 88 88
Anti-TH HPA061003 Polyclonal IHC WB 88 88
Anti-TPH2 (NTPH) AMAb91108 Monoclonal IHC 100 100
Anti-VAMP4 HPA050418 Polyclonal IHCICC-IF 100 100
Anti-VAMP7 HPA036733 Polyclonal IHCICC-IF 98 93
Products with enhanced validation for indicated application (see page 16)
NEURAL LINEAGE
A
nti-GFAP antibody
Anti-C
NP antibody
Anti-N
EFM antibodyDuring central nervous system development neural stem cells (NSCs) proliferate and divide to generate a progeny that differentiate into neurons astrocytes oligodendrocytes and different glial cells
NSCs self-renewal and differentiation are regulated by a precise temporal sequence of growth factors intracellular signaling and transcription factor expression NSCs and their differentiated progeny can be identified by the expression of a unique combination of cell surface markers and transcription factors
The glial fibrillary acidic protein (GFAP) is an intermediate filament (IF) protein and a well-known marker for astrocytes
Oligodendrocytes can convert extracellular 2rsquo3rsquo-cAMP to adenosine As CNPase is the predominant enzyme involved in this conversion the cyclic nucleotide phosphodiesterase (CNP) is widely used as an oligodendrocyte marker
Neurons express the neurofilament medium (NFEM) polypeptide other intermediate filament proteins including nestin synemin syncoilin and vimentin
Anti-GFAP polyclonal antibody (HPA056030) shows strong immunoreactivity in the
human cerebellum in brown
Anti-NEFM polyclonal antibody (HPA022845) shows positivity in a subset of neuronal cells in
human cerebral cortex in brown
Anti-CNP polyclonal antibody (HPA023266) in the rat cerebral cortex is shown in magenta
10
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Neural Lineage
Products with enhanced validation for indicated application (see page 16)
11
TargetCells
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Astrocytes Anti-GFAP AMAb91033 Monoclonal IHC WB 98 100
Astrocytes Anti-GFAP HPA056030 Polyclonal IHCWB ICC-IF 98 100
Astrocytes Anti-GLUL AMAb91101 Monoclonal IHC WB 95 53
Astrocytes Anti-GLUL AMAb91102 Monoclonal IHC WB 95 92
Astrocytes Anti-GLUL AMAb91103 Monoclonal IHC WB 95 92
Astrocytes Anti-GLUL HPA007316 Polyclonal IHCWB 95 53
Astrocytes Anti-S100B AMAb91038 Monoclonal IHC WB 99 98
Astroglia Anti-EZR AMAb90976 Monoclonal IHCWBICC-IF 93 93
Astroglia Anti-EZR HPA021616 Polyclonal IHCWBICC-IF 93 93
Adhesion molecule Anti-ICAM5 HPA009083 Polyclonal IHC 85 86
Adhesion molecule Anti-MCAM HPA008848 Polyclonal IHC WB 75 73
Adhesion molecule Anti-NCAM2 HPA030900 Polyclonal IHC 89 91
Adhesion molecule Anti-NLGN1 HPA006680 Polyclonal IHCWB 98 98
Calcium binding protein Anti-CALB1 (CB) HPA023099 Polyclonal IHCWBICC-IF 98 99
Calcium binding protein Anti-CALB2 (CR) HPA007305 Polyclonal IHCWBICC-IF 100 100
Calcium binding proteins Anti-NECAB1 AMAb90801 Monoclonal IHCWB 98 98
Calcium binding protein Anti-NECAB1 HPA023629 Polyclonal IHCWB 98 98
Calcium binding protein Anti-NECAB2 AMAb90808 Monoclonal IHC 85 84
Calcium binding protein Anti-NECAB2 HPA013998 Polyclonal IHCICC-IF 98 97
Calcium binding protein Anti-SCGN AMAb90630 Monoclonal IHCWB 96 96
Calcium binding protein Anti-SCGN HPA006641 Polyclonal IHC 96 96
Calcium binding protein Anti-S100B HPA015768 Polyclonal IHCWBICC-IF 99 98
Oligodendrocytes Anti-MOG AMAb91066 Monoclonal IHC WB 91 89
Oligodendrocytes Anti-MOG AMAb91067 Monoclonal IHC WB 91 89
Oligodendrocytes Anti-MOG HPA021873 Polyclonal IHC 91 89
Oligodendrocytes Anti-CNP AMAb91068 Monoclonal IHC WB 76 77
Oligodendrocytes Anti-CNP AMAb91069 Monoclonal IHC WB 76 77
Oligodendrocytes Anti-CNP AMAb91072 Monoclonal IHC WB ICC-IF 76 77
Oligodendrocytes Anti-CNP HPA023280 Polyclonal IHCWBICC-IF 76 77
Progenitors Anti-PBK HPA005753 Polyclonal IHCWBICC-IF 91 94
Progenitors Anti-MKI67 (Ki67) AMAb90870 Monoclonal IHC ICC-IF 68 68
Progenitors Anti-MKI67 (Ki67) HPA000451 Polyclonal IHCICC-IF 66 67
TargetCells
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Cytoskeleton Anti-ACTN1 HPA006035 Polyclonal IHCWB 98 99
Cytoskeleton Anti-ACTN4 HPA001873 Polyclonal IHCWBICC-IF 99 98
Cytoskeleton Anti-INA HPA008057 Polyclonal IHC WB ICC-IF 83 84
Cytoskeleton Anti-MAP1A HPA039064 Polyclonal IHC 60 52
Cytoskeleton Anti-MAP1B HPA022275 Polyclonal IHCWB ICC-IF 85 86
Cytoskeleton Anti-MAP2 HPA008273 Polyclonal IHC WB ICC-IF 96 96
Cytoskeleton Anti-MYO5A HPA001356 Polyclonal IHCICC-IF 99 98
Cytoskeleton Anti-NEFM (NF160) HPA022845 Polyclonal IHCWBICC-IF 98 98
Microglia Anti-AIF HPA049234 Polyclonal IHC 84 84
Microglia Anti-CD68 AMAb90874 Monoclonal IHCWB 76 76
Microglia Anti-CD68 HPA048982 Polyclonal IHC WB 76 76
Microglia Anti-ITGAM (CD11b) AMAb90911 Monoclonal IHC WB 67 68
Microglia Anti-PTPRC AMAb90518 Monoclonal IHCWB 35 37
Microglia Anti-PTPRC HPA000440 Polyclonal IHCWB 35 37
Microglia Anti-TMEM119 AMAb91528 Monoclonal IHCWB ICC-IF 52 46
Microglia Anti-TMEM119 HPA051870 Polyclonal IHC 52 46
Neurons Anti-NEFM (NF160) AMAb91027 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91028 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91029 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91030 Monoclonal IHC WB 98 98
Neurons Anti-NEFH (NF200) AMAb91025 Monoclonal IHC WB 98 98
Neurons Anti-NEFH (NF200) HPA061615 Polyclonal IHC 88 94
Neurons Anti-UCHL1 AMAb91145 Monoclonal IHC WB ICC-IF 97 97
Neurons Anti-UCHL1 HPA005993 Polyclonal IHCWBICC-IF 97 97
Neurons Anti-VIM AMAb90516 Monoclonal IHCWB 99 99
Neurons Anti-VIM HPA001762 Polyclonal IHCWBICC-IF 99 99
Schwann cells Anti-MBP AMAb91062 Monoclonal IHC WB ICC-IF 97 97
Schwann cells Anti-MBP AMAb91063 Monoclonal IHC WB ICC-IF 97 97
Schwann cells Anti-MBP AMAb91064 Monoclonal IHC WB 97 97
Anti-I
TM2B antibodyNeurodegenerative diseases such as Amyotrophic Lateral Sclerosis Parkinsonrsquos Alzheimerrsquos and Huntingtonrsquos diseases occur as a result of the progressive loss of structure and function in neurons leading to the death of neurons
Age remains the primary risk factor for almost all the neurodegenerative disorders thus interfering with age-related molecular mechanisms or pathways might be an avenue to develop new therapeutics
The choline acetyltransferase (ChAT) is an enzyme responsible for catalyzing the synthesis of acetylcholine and is expressed in the majority of cholinergic neurons Hence ChAT immunoreactivity is used to detect cognitive decline in several neurodegenerative disorders
Integral membrane protein 2B (ITM2B) is a protein that in humans is encoded by the ITM2B gene This gene located on chromosome 13 is connected to familial dementia causing amyloid and pre-fibrillar effects similar to those seen in Alzheimerrsquos
The expression of the ubiquitin-carboxyl-terminal hydrolase isozyme L1 (UCHL1) present both in the neuronal cells of the central and peripheral nervous system is down-regulated in Parkinsonrsquos and Alzheimerrsquos disease patients
NEURODEGENERATIVE DISEASES
Anti-C
HAT antibody
Anti-U
CHL1 antibody
Anti-CHAT monoclonal antibody (AMAb91130) shows strong immunoreactivity in the
acetylcholine neurons in the rat diagonal band of Broca in green
Anti-ITM2B polyclonal antibody (HPA029292) targeting Integral membrane protein 2B strongly
labels Golgi apparatus in A-431 cells in green
Anti-UCHL1 monoclonal antibody (AMAb91145) in the mouse trigeminal ganglion show strong
immunoreactrivity in primary sensory neurons in green
12
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for NeurodegenerationProduct Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-ADAR AMAb90535 Monoclonal IHCWB ICC-IF 86 85
Anti-ADAR HPA003890 Polyclonal IHCWBICC-IF 86 85
Anti-AIMP1 HPA018476 Polyclonal IHCWBICC-IF 96 97
Anti-AKT1 AMAb90834 Monoclonal WB ICC-IF 97 97
Anti-AOX1 HPA040199 Polyclonal IHC 84 86
Anti-APBA3 HPA045577 Polyclonal IHC 70 68
Anti-APBB2 HPA023542 Polyclonal IHCWBICC-IF 85 83
Anti-APBB3 HPA005571 Polyclonal IHCICC-IF 84 84
Anti-APOA4 AMAb90769 Monoclonal IHC WB 65 28
Anti-APP HPA001462 Polyclonal IHCWB 95 95
Anti-AQP4 AMAb90537 Monoclonal IHCWB 93 92
Anti-AQP4 HPA014784 Polyclonal IHCWB ICC-IF 93 92
Anti-ATF2 HPA022134 Polyclonal IHCWBICC-IF 99 99
Anti-ATF3 AMAb90909 Monoclonal IHCICC-IF 92 92
Anti-ATF3 HPA001562 Polyclonal IHCICC-IF 92 92
Anti-ATRX AMAb90784 Monoclonal IHC WBICC-IF 96 97
Anti-ATXN1 HPA008335 Polyclonal IHC 81 81
Anti-ATXN2 HPA018295 Polyclonal IHCWBICC-IF 90 91
Anti-C3 HPA020432 Polyclonal IHC 78 23
Anti-CASP3 HPA002643 Polyclonal IHCWBICC-IF 84 88
Anti-CD4 AMAb90754 Monoclonal IHCWB 55 57
Anti-CD40 AMAb90905 Monoclonal IHCWB 58 54
Anti-CHAT AMAb91130 Monoclonal IHCICC-IF 96 96
Anti-CHAT HPA048547 Polyclonal IHC 96 96
Anti-CHGA AMAb90525 Monoclonal IHCWBICC-IF 62 64
Anti-COX1 HPA002834 Polyclonal IHCWBICC-IF 93 90
Anti-COX2PTGS2 HPA001335 Polyclonal IHCICC-IF 88 88
Anti-CTSB HPA018156 Polyclonal IHCICC-IF 79 79
Anti-CTSD HPA003001 Polyclonal IHCWB 86 86
Anti-CXorf27 HPA003356 Polyclonal IHC 47 46
Anti-FBXO7 (PARK15) HPA032114 Polyclonal IHC 78 81
Anti-FUS AMAb90549 Monoclonal IHC WB ICC-IF 91 91Anti-GSK3B HPA028017 Polyclonal IHCWBICC-IF 100 100Anti-HIP1 HPA017964 Polyclonal IHCWB 79 77Anti-HTRA2 (PARK13) HPA027366 Polyclonal IHCWB 57 63Anti-ITGAM (CD11b) AMAb90911 Monoclonal IHCWB 67 68
Anti-ITM2B HPA029292 Polyclonal IHC ICC-IF 95 96Anti-LRP2 HPA005980 Polyclonal IHC 78 36
Products with enhanced validation for indicated application (see page 16)
13
Product Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-NFKB1 HPA027305 Polyclonal IHCWBICC-IF 60 62
Anti-OPTN HPA003360 Polyclonal IHCWBICC-IF 64 68
Anti-PADI4 HPA017007 Polyclonal IHCWB 66 69
Anti-PARK7 HPA004190 Polyclonal IHCWB 89 90
Anti-PFN1 AMAb91181 Monoclonal IHCWB 87 87
Anti-PHGDH AMAb90786 Monoclonal IHCWBICC-IF 99 99
Anti-PHGDH HPA021241 Polyclonal IHCWBICC-IF 99 99
Anti-PRNP HPA042754 Polyclonal IHC 91 91
Anti-PSEN1 HPA030760 Polyclonal IHC 82 81
Anti-RHOT1 AMAb90852 Monoclonal IHCWB 100 100
Anti-RHOT1 HPA010687 Polyclonal IHC 100 100
Anti-S100A8 HPA024372 Polyclonal IHCWBICC-IF 56 60
Anti-SERPINA3 HPA002560 Polyclonal IHCWB 60 59
Anti-SNCB HPA035876 Polyclonal IHCWB 97 97
Anti-SOD1 HPA001401 Polyclonal IHCWBICC-IF 82 81
Anti-SOD2 HPA001814 Polyclonal IHCWB 88 87
Anti-THY1 AMAb90844 Monoclonal IHCWB 64 68
Anti-THY1 HPA003733 Polyclonal IHCICC-IF 64 68
Anti-TNFRSF21 HPA006746 Polyclonal IHCWB 86 85
Anti-TTR AMAb90649 Monoclonal IHC WB 81 83
Anti-UBE2K HPA028869 Polyclonal IHCICC-IF 100 100
Anti-UCHL1 HPA005993 Polyclonal IHCWBICC-IF 97 97
Anti-USP46 AMAb90722 Monoclonal WBICC-IF 100 99
Anti-VPS26A AMAb90967 Monoclonal IHC WBICC-IF 96 100
Anti-WHSC1 AMAb90851 Monoclonal IHCWBICC-IF 91 91
Anti-WHSC1 HPA015801 Polyclonal IHCICC-IF 91 91
Anti-VWF AMAb90928 Monoclonal IHCWB 80 80
Anti-VWF HPA002082 Polyclonal IHC 82 78
The development of the central nervous system includes the development of the brain spinal cord optic and auditory systems
Within the neural tube stem cells generate the two major classes of cells that make the majority of the nervous system neurons and glia Both these classes of cells differentiate into many different types generated with highly specialized functions and shapes
The development of the peripheral and central nervous systems begins early in embryogenesis and can be traced through the use of lineage antibodies specific to each stage of neuronal development
The (SOX) family of transcription factors are well-established regulators of cell fate decisions during development
Vimentin (VIM) is a type III intermediate filament protein expressed in mesenchymal cells It is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both healthy development and metastatic progression
The cell surface glycoprotein Thy1 (CD90) is expressed on many mammalian cell types including neurons and serves as a human mesenchymal stem cell (MSC) marker
DEVELOPMENT
A
nti-VIM antibody
A
nti-SOX4 antibody
Anti-T
HY1 antibody
Anti-VIM polyclonal antibody (HPA001762) immunofluorescent staining of human cell line
ASC TERT1 shows localization to intermediate filaments in green
Anti-SOX4 monoclonal antibody (AMAb913802) immunofluorescent staining in mouse
hippocampus showing immunoreactivity in a subset of neurons in the granule cell layer of
the dentate gyrus in brown
Anti-THY1 polyclonal antibody (HPA003733) staining of human cerebral cortex shows
localization to neuropils in brown
Product Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-ENG AMAb90925 Monoclonal IHC 66 22Anti-ENG HPA011862 Polyclonal IHCWB 66 22Anti-FABP7 AMAb90595 Monoclonal IHCWB 89 90Anti-FABP7 HPA028825 Polyclonal IHCWB 89 90Anti-FLT1 AMAb90704 Monoclonal IHCWB 80 82Anti-GAP43 HPA015600 Polyclonal IHC ICC-IF 71 70Anti-GLI3 HPA005534 Polyclonal IHC 74 76Anti-MEF2C AMAb90727 Monoclonal IHCWBICC-IF 97 47Anti-MEF2C HPA005533 Polyclonal IHCWBICC-IF 97 47Anti-MKI67 (Ki67) HPA000451 Polyclonal IHCICC-IF 66 67Anti-NACC1 HPA021238 Polyclonal IHCICC-IF 91 89Anti-NES (Nestin) AMAb90556 Monoclonal IHCWBICC-IF 47 42Anti-NES (Nestin) HPA007007 Polyclonal IHCWB 47 42Anti-NKX2-2 HPA003468 Polyclonal IHCWB 96 97Anti-PAX6 HPA030775 Polyclonal IHCICC-IF 100 100Anti-PBK HPA005753 Polyclonal IHCWBICC-IF 91 94Anti-REST AMAb90740 Monoclonal IHCICC-IF 41 43Anti-RUNX1 HPA004176 Polyclonal IHCICC-IF 93 93Anti-RUNX2 AMAb90591 Monoclonal IHCWBICC-IF 100 81Anti-RUNX2 HPA022040 Polyclonal IHCWBICC-IF 100 81Anti-SATB2 AMAb90679 Monoclonal IHCWBICC-IF 100 100Anti-SATB2 HPA029543 Polyclonal IHCWBICC-IF 100 100Anti-SOX4 HPA029901 Polyclonal IHCICC-IF 100 39Anti-SOX6 HPA001923 Polyclonal IHCICC-IF 96 96Anti-SOX7 HPA009065 Polyclonal IHCWB 91 91Anti-SOX9 AMAb90795 Monoclonal IHCWBICC-IF 97 96Anti-SOX11 AMAb90502 Monoclonal IHCWB 82 82Anti-SOX11 HPA000536 Polyclonal IHCWB 82 82Anti-SOX30 HPA006159 Polyclonal IHCWB 68 70Anti-THY1 AMAb90844 Monoclonal IHCWB 64 68Anti-THY1 HPA003733 Polyclonal IHCICC-IF 64 68Anti-TM4SF2TSPAN7 HPA003140 Polyclonal IHCWB 96 96Anti-TM4SF2TSPAN7 AMAb90621 Monoclonal IHCWB 96 96Anti-VANGL1 AMAb90600 Monoclonal WBICC-IF 95 95Anti-VIM AMAb90516 Monoclonal IHCWB 99 99Anti-VIM HPA001762 Polyclonal IHCWBICC-IF 99 99
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Development
Products with enhanced validation for indicated application (see page 16)
15
At Atlas Antibodies we extensively validate our antibodies in IHC WB and ICC-IF Enhanced Validation is performed as an additional layer of security in an application and context-specific manner Enhanced validation offers increased security of antibody specificity in a defined context This is ensured by using the most relevant validation method for each combination of protein sample and application Enhanced Validation follows the guidelines proposed by the International Working Group for Antibody Validation (IWGAV)
ENHANCED VALIDATION
GENETIC VALIDATION Antibody specificity is confirmed by downregulating the target protein on a genetic level using siRNA
ORTHOGONAL VALIDATIONThe antibody is validated by comparing the results with a non-antibody based method across multiple samples
VALIDATION BY INDEPENDENT ANTIBODIESThe antibody specificity is demonstrated by comparing two antibodies targeting different regions of the same protein
RECOMBINANT EXPRESSION VALIDATIONThe antibody binding is verified using and over-expressed or tagged version of the target protein
MIGRATION CAPTURE MS VALIDATIONThe staining pattern and the protein size detected by the antibody is compared with results obtained by a capture Mass Spectrometry (MS) method
16
Triple A PolyclonalsTM are rabbit polyclonal primary antibodies developed within the Human Protein Atlas project IHC characterization data from 44 normal and 20 cancer tissues is available on the Human Protein Atlas portal
PrecisA MonoclonalsTM are mouse monoclonal primary antibodies developed against a number of carefully selected targets Clones are selected to recognize only unique non-overlapping epitopes and isotypes
Atlas Antibodies ABVoltavaumlgen 13SE-168 69 BrommaSwedenatlasantibodiescom
Phone +46(0)8 54 59 58 50contactatlasantibodiescomorderatlasantibodiescomsupportatlasantibodiescom
Atlas Antibodies logo Triple A Polyclonals PrecisA Monoclonals PrEST Antigen and QPrEST are trademarks or registered trademarks of Atlas Antibodies AB All other trademarks are the property of their respective owners
Products are for research use only Not for use in diagnostic procedures copy Atlas Antibodies AB 2020 008_
Neu
ro M
arke
r Pan
el _
Rev
03_2
0121
5_S
fN E
ditio
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Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Synaptic Signaling
9
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Anti-ATF2 HPA022134 Polyclonal IHC WB ICC-IF 99 99
Anti-ATF3 AMAb90909 Monoclonal IHC ICC-IF 92 92
Anti-ATF3 HPA001562 Polyclonal IHC ICC-IF 92 92
Anti-ATP1B1 HPA012911 Polyclonal IHC WB 93 93
Anti-ATP1B2 HPA010698 Polyclonal IHC 96 88
Anti-CAMK2B HPA026307 Polyclonal IHC WB 96 96
Anti-CALB1 HPA023099 Polyclonal IHC WB ICC-IF 98 99
Anti-CALB2 HPA007306 Polyclonal IHC WB ICC-IF 100 100
Anti-CALB2 HPA007305 Polyclonal IHC WB ICC-IF 100 100
Anti-CAMK2D HPA026281 Polyclonal IHC 100 97
Anti-C-FOS HPA018531 Polyclonal IHC ICC-IF 94 94
Anti-CHAT AMAb91130 Monoclonal IHC ICC-IF 96 96
Anti-CHAT HPA048547 Polyclonal IHC 96 96
Anti-CHRM1 (M1 mAChR) HPA014101 Polyclonal IHC 98 97
Anti-CHRM2 (M2 AChR) HPA029795 Polyclonal IHC 88 86
Anti-CLIC4 HPA008019 Polyclonal IHC WB 98 97
Anti-CREB1 HPA019150 Polyclonal IHC WB ICC-IF 100 100
Anti-DDC AMAb91089 Monoclonal IHCWB 90 88
Anti-DDC HPA017742 Polyclonal IHCWBICC-IF 90 88
Anti-EAAC1 HPA020086 Polyclonal IHC WB 77 79
Anti-EAAT2 HPA009172 Polyclonal IHC 87 89
Anti-GABRA3 HPA000839 Polyclonal IHCWB 91 93
Anti-GABRB1 HPA051297 Polyclonal IHC 97 100
Anti-GABRG1 HPA035622 Polyclonal IHC 96 94
Anti-GAD1 (GAD67) AMAb91076 Monoclonal IHCWB 94 94
Anti-GAD1 HPA058412 Polyclonal IHCWB 94 94
Anti-GAD2 AMAb91048 Monoclonal IHCWB 84 88
Anti-GAD2 HPA044637 Polyclonal IHC 84 88
Anti-GAT1 HPA013341 Polyclonal IHC WB 98 98
Anti-GAT3 HPA037981 Polyclonal IHCWB 85 87
Anti-GLUR2 (AMPA2) HPA008441 Polyclonal IHC 100 100
Anti-HTR2A HPA014011 Polyclonal IHC 95 97
Anti-KCC4 HPA041652 Polyclonal IHCWB 84 82
Anti-KCNJ5 (KIR34) HPA017353 Polyclonal IHCWB 89 89
Anti-KIF11 HPA010568 Polyclonal IHCWB 88 83
Anti-KIF17 HPA032085 Polyclonal IHCICC-IF 85 82
Anti-KIF18A HPA039312 Polyclonal IHCWBICC-IF 80 82
Anti-KIF1C HPA024602 Polyclonal IHCWB 81 83
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Anti-KIF26B HPA028562 Polyclonal IHCICC-IF 88 80
Anti-KIF4A (KIF4A amp B) HPA034745 Polyclonal IHCWBICC-IF 64 63
Anti-KIF5A HPA004469 Polyclonal IHCWB 91 88
Anti-KIF5C HPA035210 Polyclonal IHCWB 100100
Anti-KIF7 HPA043145 Polyclonal IHC 69 69
Anti-KIFAP3 HPA023742 Polyclonal IHC 100 100
Anti-MAPK1 (ERK) HPA030069 Polyclonal IHCWB 100 100
Anti-MAPK3 (ERK1) HPA005700 Polyclonal IHCWB 98 98
Anti-MGLUR1 HPA015701 Polyclonal IHC 80 80
Anti-MGLUR8 HPA051481 Polyclonal IHC 95 90
Anti-NCS1 HPA019713 Polyclonal IHCWBICC-IF 100 100
Anti-PRKCA HPA006563 Polyclonal IHCWBICC-IF 99 99
Anti-PRKCH HPA053709 Polyclonal IHCICC-IF 97 64
Anti-PNMT HPA051005 Polyclonal IHC 89 92
Anti-PRKACB (PKACB) HPA029754 Polyclonal IHC 73 76
Anti-PRKCZ HPA021851 Polyclonal IHCWB ICC-IF 94 94
Anti-RAB3A HPA003160 Polyclonal IHC 99 99
Anti-RAP1GAP HPA001922 Polyclonal IHC 92 91
Anti-SLC17A7 (VGLUT1) AMAb91041 Monoclonal IHCWB 94 94
Anti-SLC22A2 HPA008567 Polyclonal IHC 84 77
Anti-SLC32A1 (VGAT) HPA058859 Polyclonal IHC 95 93
Anti-SLC6A2 (NET) AMAb91116 Monoclonal IHC -
Anti-SLC6A3 (DAT) AMAb91125 Monoclonal IHC 85 85
Anti-SNAP25 HPA001830 Polyclonal IHCWB 100 100
Anti-SNAP29 HPA031823 Polyclonal IHC 89 92
Anti-SST (SOM) HPA019472 Polyclonal IHCWB 98 98
Anti-STXBP1 HPA008209 Polyclonal IHCWBICC-IF 100 100
Anti-STXBP6 HPA003552 Polyclonal IHCWB 99 99
Anti-SYNGR1 HPA029673 Polyclonal IHC ICC-IF 90 88
Anti-SYNPR HPA061671 Polyclonal IHC 97 97
Anti-SYP HPA002858 Polyclonal IHCWB 83 83
Anti-SYT1 HPA008394 Polyclonal IHCWB 100 100
Anti-SYT12 HPA011006 Polyclonal IHC 96 98
Anti-SYT13 HPA046224 Polyclonal IHC 96 93
Anti-SYT16 HPA004199 Polyclonal IHCWB 95 95
Anti-TGFA HPA042297 Polyclonal IHC 93 93
Anti-TH AMAb91112 Monoclonal IHC 88 88
Anti-TH HPA061003 Polyclonal IHC WB 88 88
Anti-TPH2 (NTPH) AMAb91108 Monoclonal IHC 100 100
Anti-VAMP4 HPA050418 Polyclonal IHCICC-IF 100 100
Anti-VAMP7 HPA036733 Polyclonal IHCICC-IF 98 93
Products with enhanced validation for indicated application (see page 16)
NEURAL LINEAGE
A
nti-GFAP antibody
Anti-C
NP antibody
Anti-N
EFM antibodyDuring central nervous system development neural stem cells (NSCs) proliferate and divide to generate a progeny that differentiate into neurons astrocytes oligodendrocytes and different glial cells
NSCs self-renewal and differentiation are regulated by a precise temporal sequence of growth factors intracellular signaling and transcription factor expression NSCs and their differentiated progeny can be identified by the expression of a unique combination of cell surface markers and transcription factors
The glial fibrillary acidic protein (GFAP) is an intermediate filament (IF) protein and a well-known marker for astrocytes
Oligodendrocytes can convert extracellular 2rsquo3rsquo-cAMP to adenosine As CNPase is the predominant enzyme involved in this conversion the cyclic nucleotide phosphodiesterase (CNP) is widely used as an oligodendrocyte marker
Neurons express the neurofilament medium (NFEM) polypeptide other intermediate filament proteins including nestin synemin syncoilin and vimentin
Anti-GFAP polyclonal antibody (HPA056030) shows strong immunoreactivity in the
human cerebellum in brown
Anti-NEFM polyclonal antibody (HPA022845) shows positivity in a subset of neuronal cells in
human cerebral cortex in brown
Anti-CNP polyclonal antibody (HPA023266) in the rat cerebral cortex is shown in magenta
10
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Neural Lineage
Products with enhanced validation for indicated application (see page 16)
11
TargetCells
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Astrocytes Anti-GFAP AMAb91033 Monoclonal IHC WB 98 100
Astrocytes Anti-GFAP HPA056030 Polyclonal IHCWB ICC-IF 98 100
Astrocytes Anti-GLUL AMAb91101 Monoclonal IHC WB 95 53
Astrocytes Anti-GLUL AMAb91102 Monoclonal IHC WB 95 92
Astrocytes Anti-GLUL AMAb91103 Monoclonal IHC WB 95 92
Astrocytes Anti-GLUL HPA007316 Polyclonal IHCWB 95 53
Astrocytes Anti-S100B AMAb91038 Monoclonal IHC WB 99 98
Astroglia Anti-EZR AMAb90976 Monoclonal IHCWBICC-IF 93 93
Astroglia Anti-EZR HPA021616 Polyclonal IHCWBICC-IF 93 93
Adhesion molecule Anti-ICAM5 HPA009083 Polyclonal IHC 85 86
Adhesion molecule Anti-MCAM HPA008848 Polyclonal IHC WB 75 73
Adhesion molecule Anti-NCAM2 HPA030900 Polyclonal IHC 89 91
Adhesion molecule Anti-NLGN1 HPA006680 Polyclonal IHCWB 98 98
Calcium binding protein Anti-CALB1 (CB) HPA023099 Polyclonal IHCWBICC-IF 98 99
Calcium binding protein Anti-CALB2 (CR) HPA007305 Polyclonal IHCWBICC-IF 100 100
Calcium binding proteins Anti-NECAB1 AMAb90801 Monoclonal IHCWB 98 98
Calcium binding protein Anti-NECAB1 HPA023629 Polyclonal IHCWB 98 98
Calcium binding protein Anti-NECAB2 AMAb90808 Monoclonal IHC 85 84
Calcium binding protein Anti-NECAB2 HPA013998 Polyclonal IHCICC-IF 98 97
Calcium binding protein Anti-SCGN AMAb90630 Monoclonal IHCWB 96 96
Calcium binding protein Anti-SCGN HPA006641 Polyclonal IHC 96 96
Calcium binding protein Anti-S100B HPA015768 Polyclonal IHCWBICC-IF 99 98
Oligodendrocytes Anti-MOG AMAb91066 Monoclonal IHC WB 91 89
Oligodendrocytes Anti-MOG AMAb91067 Monoclonal IHC WB 91 89
Oligodendrocytes Anti-MOG HPA021873 Polyclonal IHC 91 89
Oligodendrocytes Anti-CNP AMAb91068 Monoclonal IHC WB 76 77
Oligodendrocytes Anti-CNP AMAb91069 Monoclonal IHC WB 76 77
Oligodendrocytes Anti-CNP AMAb91072 Monoclonal IHC WB ICC-IF 76 77
Oligodendrocytes Anti-CNP HPA023280 Polyclonal IHCWBICC-IF 76 77
Progenitors Anti-PBK HPA005753 Polyclonal IHCWBICC-IF 91 94
Progenitors Anti-MKI67 (Ki67) AMAb90870 Monoclonal IHC ICC-IF 68 68
Progenitors Anti-MKI67 (Ki67) HPA000451 Polyclonal IHCICC-IF 66 67
TargetCells
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Cytoskeleton Anti-ACTN1 HPA006035 Polyclonal IHCWB 98 99
Cytoskeleton Anti-ACTN4 HPA001873 Polyclonal IHCWBICC-IF 99 98
Cytoskeleton Anti-INA HPA008057 Polyclonal IHC WB ICC-IF 83 84
Cytoskeleton Anti-MAP1A HPA039064 Polyclonal IHC 60 52
Cytoskeleton Anti-MAP1B HPA022275 Polyclonal IHCWB ICC-IF 85 86
Cytoskeleton Anti-MAP2 HPA008273 Polyclonal IHC WB ICC-IF 96 96
Cytoskeleton Anti-MYO5A HPA001356 Polyclonal IHCICC-IF 99 98
Cytoskeleton Anti-NEFM (NF160) HPA022845 Polyclonal IHCWBICC-IF 98 98
Microglia Anti-AIF HPA049234 Polyclonal IHC 84 84
Microglia Anti-CD68 AMAb90874 Monoclonal IHCWB 76 76
Microglia Anti-CD68 HPA048982 Polyclonal IHC WB 76 76
Microglia Anti-ITGAM (CD11b) AMAb90911 Monoclonal IHC WB 67 68
Microglia Anti-PTPRC AMAb90518 Monoclonal IHCWB 35 37
Microglia Anti-PTPRC HPA000440 Polyclonal IHCWB 35 37
Microglia Anti-TMEM119 AMAb91528 Monoclonal IHCWB ICC-IF 52 46
Microglia Anti-TMEM119 HPA051870 Polyclonal IHC 52 46
Neurons Anti-NEFM (NF160) AMAb91027 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91028 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91029 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91030 Monoclonal IHC WB 98 98
Neurons Anti-NEFH (NF200) AMAb91025 Monoclonal IHC WB 98 98
Neurons Anti-NEFH (NF200) HPA061615 Polyclonal IHC 88 94
Neurons Anti-UCHL1 AMAb91145 Monoclonal IHC WB ICC-IF 97 97
Neurons Anti-UCHL1 HPA005993 Polyclonal IHCWBICC-IF 97 97
Neurons Anti-VIM AMAb90516 Monoclonal IHCWB 99 99
Neurons Anti-VIM HPA001762 Polyclonal IHCWBICC-IF 99 99
Schwann cells Anti-MBP AMAb91062 Monoclonal IHC WB ICC-IF 97 97
Schwann cells Anti-MBP AMAb91063 Monoclonal IHC WB ICC-IF 97 97
Schwann cells Anti-MBP AMAb91064 Monoclonal IHC WB 97 97
Anti-I
TM2B antibodyNeurodegenerative diseases such as Amyotrophic Lateral Sclerosis Parkinsonrsquos Alzheimerrsquos and Huntingtonrsquos diseases occur as a result of the progressive loss of structure and function in neurons leading to the death of neurons
Age remains the primary risk factor for almost all the neurodegenerative disorders thus interfering with age-related molecular mechanisms or pathways might be an avenue to develop new therapeutics
The choline acetyltransferase (ChAT) is an enzyme responsible for catalyzing the synthesis of acetylcholine and is expressed in the majority of cholinergic neurons Hence ChAT immunoreactivity is used to detect cognitive decline in several neurodegenerative disorders
Integral membrane protein 2B (ITM2B) is a protein that in humans is encoded by the ITM2B gene This gene located on chromosome 13 is connected to familial dementia causing amyloid and pre-fibrillar effects similar to those seen in Alzheimerrsquos
The expression of the ubiquitin-carboxyl-terminal hydrolase isozyme L1 (UCHL1) present both in the neuronal cells of the central and peripheral nervous system is down-regulated in Parkinsonrsquos and Alzheimerrsquos disease patients
NEURODEGENERATIVE DISEASES
Anti-C
HAT antibody
Anti-U
CHL1 antibody
Anti-CHAT monoclonal antibody (AMAb91130) shows strong immunoreactivity in the
acetylcholine neurons in the rat diagonal band of Broca in green
Anti-ITM2B polyclonal antibody (HPA029292) targeting Integral membrane protein 2B strongly
labels Golgi apparatus in A-431 cells in green
Anti-UCHL1 monoclonal antibody (AMAb91145) in the mouse trigeminal ganglion show strong
immunoreactrivity in primary sensory neurons in green
12
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for NeurodegenerationProduct Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-ADAR AMAb90535 Monoclonal IHCWB ICC-IF 86 85
Anti-ADAR HPA003890 Polyclonal IHCWBICC-IF 86 85
Anti-AIMP1 HPA018476 Polyclonal IHCWBICC-IF 96 97
Anti-AKT1 AMAb90834 Monoclonal WB ICC-IF 97 97
Anti-AOX1 HPA040199 Polyclonal IHC 84 86
Anti-APBA3 HPA045577 Polyclonal IHC 70 68
Anti-APBB2 HPA023542 Polyclonal IHCWBICC-IF 85 83
Anti-APBB3 HPA005571 Polyclonal IHCICC-IF 84 84
Anti-APOA4 AMAb90769 Monoclonal IHC WB 65 28
Anti-APP HPA001462 Polyclonal IHCWB 95 95
Anti-AQP4 AMAb90537 Monoclonal IHCWB 93 92
Anti-AQP4 HPA014784 Polyclonal IHCWB ICC-IF 93 92
Anti-ATF2 HPA022134 Polyclonal IHCWBICC-IF 99 99
Anti-ATF3 AMAb90909 Monoclonal IHCICC-IF 92 92
Anti-ATF3 HPA001562 Polyclonal IHCICC-IF 92 92
Anti-ATRX AMAb90784 Monoclonal IHC WBICC-IF 96 97
Anti-ATXN1 HPA008335 Polyclonal IHC 81 81
Anti-ATXN2 HPA018295 Polyclonal IHCWBICC-IF 90 91
Anti-C3 HPA020432 Polyclonal IHC 78 23
Anti-CASP3 HPA002643 Polyclonal IHCWBICC-IF 84 88
Anti-CD4 AMAb90754 Monoclonal IHCWB 55 57
Anti-CD40 AMAb90905 Monoclonal IHCWB 58 54
Anti-CHAT AMAb91130 Monoclonal IHCICC-IF 96 96
Anti-CHAT HPA048547 Polyclonal IHC 96 96
Anti-CHGA AMAb90525 Monoclonal IHCWBICC-IF 62 64
Anti-COX1 HPA002834 Polyclonal IHCWBICC-IF 93 90
Anti-COX2PTGS2 HPA001335 Polyclonal IHCICC-IF 88 88
Anti-CTSB HPA018156 Polyclonal IHCICC-IF 79 79
Anti-CTSD HPA003001 Polyclonal IHCWB 86 86
Anti-CXorf27 HPA003356 Polyclonal IHC 47 46
Anti-FBXO7 (PARK15) HPA032114 Polyclonal IHC 78 81
Anti-FUS AMAb90549 Monoclonal IHC WB ICC-IF 91 91Anti-GSK3B HPA028017 Polyclonal IHCWBICC-IF 100 100Anti-HIP1 HPA017964 Polyclonal IHCWB 79 77Anti-HTRA2 (PARK13) HPA027366 Polyclonal IHCWB 57 63Anti-ITGAM (CD11b) AMAb90911 Monoclonal IHCWB 67 68
Anti-ITM2B HPA029292 Polyclonal IHC ICC-IF 95 96Anti-LRP2 HPA005980 Polyclonal IHC 78 36
Products with enhanced validation for indicated application (see page 16)
13
Product Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-NFKB1 HPA027305 Polyclonal IHCWBICC-IF 60 62
Anti-OPTN HPA003360 Polyclonal IHCWBICC-IF 64 68
Anti-PADI4 HPA017007 Polyclonal IHCWB 66 69
Anti-PARK7 HPA004190 Polyclonal IHCWB 89 90
Anti-PFN1 AMAb91181 Monoclonal IHCWB 87 87
Anti-PHGDH AMAb90786 Monoclonal IHCWBICC-IF 99 99
Anti-PHGDH HPA021241 Polyclonal IHCWBICC-IF 99 99
Anti-PRNP HPA042754 Polyclonal IHC 91 91
Anti-PSEN1 HPA030760 Polyclonal IHC 82 81
Anti-RHOT1 AMAb90852 Monoclonal IHCWB 100 100
Anti-RHOT1 HPA010687 Polyclonal IHC 100 100
Anti-S100A8 HPA024372 Polyclonal IHCWBICC-IF 56 60
Anti-SERPINA3 HPA002560 Polyclonal IHCWB 60 59
Anti-SNCB HPA035876 Polyclonal IHCWB 97 97
Anti-SOD1 HPA001401 Polyclonal IHCWBICC-IF 82 81
Anti-SOD2 HPA001814 Polyclonal IHCWB 88 87
Anti-THY1 AMAb90844 Monoclonal IHCWB 64 68
Anti-THY1 HPA003733 Polyclonal IHCICC-IF 64 68
Anti-TNFRSF21 HPA006746 Polyclonal IHCWB 86 85
Anti-TTR AMAb90649 Monoclonal IHC WB 81 83
Anti-UBE2K HPA028869 Polyclonal IHCICC-IF 100 100
Anti-UCHL1 HPA005993 Polyclonal IHCWBICC-IF 97 97
Anti-USP46 AMAb90722 Monoclonal WBICC-IF 100 99
Anti-VPS26A AMAb90967 Monoclonal IHC WBICC-IF 96 100
Anti-WHSC1 AMAb90851 Monoclonal IHCWBICC-IF 91 91
Anti-WHSC1 HPA015801 Polyclonal IHCICC-IF 91 91
Anti-VWF AMAb90928 Monoclonal IHCWB 80 80
Anti-VWF HPA002082 Polyclonal IHC 82 78
The development of the central nervous system includes the development of the brain spinal cord optic and auditory systems
Within the neural tube stem cells generate the two major classes of cells that make the majority of the nervous system neurons and glia Both these classes of cells differentiate into many different types generated with highly specialized functions and shapes
The development of the peripheral and central nervous systems begins early in embryogenesis and can be traced through the use of lineage antibodies specific to each stage of neuronal development
The (SOX) family of transcription factors are well-established regulators of cell fate decisions during development
Vimentin (VIM) is a type III intermediate filament protein expressed in mesenchymal cells It is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both healthy development and metastatic progression
The cell surface glycoprotein Thy1 (CD90) is expressed on many mammalian cell types including neurons and serves as a human mesenchymal stem cell (MSC) marker
DEVELOPMENT
A
nti-VIM antibody
A
nti-SOX4 antibody
Anti-T
HY1 antibody
Anti-VIM polyclonal antibody (HPA001762) immunofluorescent staining of human cell line
ASC TERT1 shows localization to intermediate filaments in green
Anti-SOX4 monoclonal antibody (AMAb913802) immunofluorescent staining in mouse
hippocampus showing immunoreactivity in a subset of neurons in the granule cell layer of
the dentate gyrus in brown
Anti-THY1 polyclonal antibody (HPA003733) staining of human cerebral cortex shows
localization to neuropils in brown
Product Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-ENG AMAb90925 Monoclonal IHC 66 22Anti-ENG HPA011862 Polyclonal IHCWB 66 22Anti-FABP7 AMAb90595 Monoclonal IHCWB 89 90Anti-FABP7 HPA028825 Polyclonal IHCWB 89 90Anti-FLT1 AMAb90704 Monoclonal IHCWB 80 82Anti-GAP43 HPA015600 Polyclonal IHC ICC-IF 71 70Anti-GLI3 HPA005534 Polyclonal IHC 74 76Anti-MEF2C AMAb90727 Monoclonal IHCWBICC-IF 97 47Anti-MEF2C HPA005533 Polyclonal IHCWBICC-IF 97 47Anti-MKI67 (Ki67) HPA000451 Polyclonal IHCICC-IF 66 67Anti-NACC1 HPA021238 Polyclonal IHCICC-IF 91 89Anti-NES (Nestin) AMAb90556 Monoclonal IHCWBICC-IF 47 42Anti-NES (Nestin) HPA007007 Polyclonal IHCWB 47 42Anti-NKX2-2 HPA003468 Polyclonal IHCWB 96 97Anti-PAX6 HPA030775 Polyclonal IHCICC-IF 100 100Anti-PBK HPA005753 Polyclonal IHCWBICC-IF 91 94Anti-REST AMAb90740 Monoclonal IHCICC-IF 41 43Anti-RUNX1 HPA004176 Polyclonal IHCICC-IF 93 93Anti-RUNX2 AMAb90591 Monoclonal IHCWBICC-IF 100 81Anti-RUNX2 HPA022040 Polyclonal IHCWBICC-IF 100 81Anti-SATB2 AMAb90679 Monoclonal IHCWBICC-IF 100 100Anti-SATB2 HPA029543 Polyclonal IHCWBICC-IF 100 100Anti-SOX4 HPA029901 Polyclonal IHCICC-IF 100 39Anti-SOX6 HPA001923 Polyclonal IHCICC-IF 96 96Anti-SOX7 HPA009065 Polyclonal IHCWB 91 91Anti-SOX9 AMAb90795 Monoclonal IHCWBICC-IF 97 96Anti-SOX11 AMAb90502 Monoclonal IHCWB 82 82Anti-SOX11 HPA000536 Polyclonal IHCWB 82 82Anti-SOX30 HPA006159 Polyclonal IHCWB 68 70Anti-THY1 AMAb90844 Monoclonal IHCWB 64 68Anti-THY1 HPA003733 Polyclonal IHCICC-IF 64 68Anti-TM4SF2TSPAN7 HPA003140 Polyclonal IHCWB 96 96Anti-TM4SF2TSPAN7 AMAb90621 Monoclonal IHCWB 96 96Anti-VANGL1 AMAb90600 Monoclonal WBICC-IF 95 95Anti-VIM AMAb90516 Monoclonal IHCWB 99 99Anti-VIM HPA001762 Polyclonal IHCWBICC-IF 99 99
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Development
Products with enhanced validation for indicated application (see page 16)
15
At Atlas Antibodies we extensively validate our antibodies in IHC WB and ICC-IF Enhanced Validation is performed as an additional layer of security in an application and context-specific manner Enhanced validation offers increased security of antibody specificity in a defined context This is ensured by using the most relevant validation method for each combination of protein sample and application Enhanced Validation follows the guidelines proposed by the International Working Group for Antibody Validation (IWGAV)
ENHANCED VALIDATION
GENETIC VALIDATION Antibody specificity is confirmed by downregulating the target protein on a genetic level using siRNA
ORTHOGONAL VALIDATIONThe antibody is validated by comparing the results with a non-antibody based method across multiple samples
VALIDATION BY INDEPENDENT ANTIBODIESThe antibody specificity is demonstrated by comparing two antibodies targeting different regions of the same protein
RECOMBINANT EXPRESSION VALIDATIONThe antibody binding is verified using and over-expressed or tagged version of the target protein
MIGRATION CAPTURE MS VALIDATIONThe staining pattern and the protein size detected by the antibody is compared with results obtained by a capture Mass Spectrometry (MS) method
16
Triple A PolyclonalsTM are rabbit polyclonal primary antibodies developed within the Human Protein Atlas project IHC characterization data from 44 normal and 20 cancer tissues is available on the Human Protein Atlas portal
PrecisA MonoclonalsTM are mouse monoclonal primary antibodies developed against a number of carefully selected targets Clones are selected to recognize only unique non-overlapping epitopes and isotypes
Atlas Antibodies ABVoltavaumlgen 13SE-168 69 BrommaSwedenatlasantibodiescom
Phone +46(0)8 54 59 58 50contactatlasantibodiescomorderatlasantibodiescomsupportatlasantibodiescom
Atlas Antibodies logo Triple A Polyclonals PrecisA Monoclonals PrEST Antigen and QPrEST are trademarks or registered trademarks of Atlas Antibodies AB All other trademarks are the property of their respective owners
Products are for research use only Not for use in diagnostic procedures copy Atlas Antibodies AB 2020 008_
Neu
ro M
arke
r Pan
el _
Rev
03_2
0121
5_S
fN E
ditio
n
NEURAL LINEAGE
A
nti-GFAP antibody
Anti-C
NP antibody
Anti-N
EFM antibodyDuring central nervous system development neural stem cells (NSCs) proliferate and divide to generate a progeny that differentiate into neurons astrocytes oligodendrocytes and different glial cells
NSCs self-renewal and differentiation are regulated by a precise temporal sequence of growth factors intracellular signaling and transcription factor expression NSCs and their differentiated progeny can be identified by the expression of a unique combination of cell surface markers and transcription factors
The glial fibrillary acidic protein (GFAP) is an intermediate filament (IF) protein and a well-known marker for astrocytes
Oligodendrocytes can convert extracellular 2rsquo3rsquo-cAMP to adenosine As CNPase is the predominant enzyme involved in this conversion the cyclic nucleotide phosphodiesterase (CNP) is widely used as an oligodendrocyte marker
Neurons express the neurofilament medium (NFEM) polypeptide other intermediate filament proteins including nestin synemin syncoilin and vimentin
Anti-GFAP polyclonal antibody (HPA056030) shows strong immunoreactivity in the
human cerebellum in brown
Anti-NEFM polyclonal antibody (HPA022845) shows positivity in a subset of neuronal cells in
human cerebral cortex in brown
Anti-CNP polyclonal antibody (HPA023266) in the rat cerebral cortex is shown in magenta
10
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Neural Lineage
Products with enhanced validation for indicated application (see page 16)
11
TargetCells
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Astrocytes Anti-GFAP AMAb91033 Monoclonal IHC WB 98 100
Astrocytes Anti-GFAP HPA056030 Polyclonal IHCWB ICC-IF 98 100
Astrocytes Anti-GLUL AMAb91101 Monoclonal IHC WB 95 53
Astrocytes Anti-GLUL AMAb91102 Monoclonal IHC WB 95 92
Astrocytes Anti-GLUL AMAb91103 Monoclonal IHC WB 95 92
Astrocytes Anti-GLUL HPA007316 Polyclonal IHCWB 95 53
Astrocytes Anti-S100B AMAb91038 Monoclonal IHC WB 99 98
Astroglia Anti-EZR AMAb90976 Monoclonal IHCWBICC-IF 93 93
Astroglia Anti-EZR HPA021616 Polyclonal IHCWBICC-IF 93 93
Adhesion molecule Anti-ICAM5 HPA009083 Polyclonal IHC 85 86
Adhesion molecule Anti-MCAM HPA008848 Polyclonal IHC WB 75 73
Adhesion molecule Anti-NCAM2 HPA030900 Polyclonal IHC 89 91
Adhesion molecule Anti-NLGN1 HPA006680 Polyclonal IHCWB 98 98
Calcium binding protein Anti-CALB1 (CB) HPA023099 Polyclonal IHCWBICC-IF 98 99
Calcium binding protein Anti-CALB2 (CR) HPA007305 Polyclonal IHCWBICC-IF 100 100
Calcium binding proteins Anti-NECAB1 AMAb90801 Monoclonal IHCWB 98 98
Calcium binding protein Anti-NECAB1 HPA023629 Polyclonal IHCWB 98 98
Calcium binding protein Anti-NECAB2 AMAb90808 Monoclonal IHC 85 84
Calcium binding protein Anti-NECAB2 HPA013998 Polyclonal IHCICC-IF 98 97
Calcium binding protein Anti-SCGN AMAb90630 Monoclonal IHCWB 96 96
Calcium binding protein Anti-SCGN HPA006641 Polyclonal IHC 96 96
Calcium binding protein Anti-S100B HPA015768 Polyclonal IHCWBICC-IF 99 98
Oligodendrocytes Anti-MOG AMAb91066 Monoclonal IHC WB 91 89
Oligodendrocytes Anti-MOG AMAb91067 Monoclonal IHC WB 91 89
Oligodendrocytes Anti-MOG HPA021873 Polyclonal IHC 91 89
Oligodendrocytes Anti-CNP AMAb91068 Monoclonal IHC WB 76 77
Oligodendrocytes Anti-CNP AMAb91069 Monoclonal IHC WB 76 77
Oligodendrocytes Anti-CNP AMAb91072 Monoclonal IHC WB ICC-IF 76 77
Oligodendrocytes Anti-CNP HPA023280 Polyclonal IHCWBICC-IF 76 77
Progenitors Anti-PBK HPA005753 Polyclonal IHCWBICC-IF 91 94
Progenitors Anti-MKI67 (Ki67) AMAb90870 Monoclonal IHC ICC-IF 68 68
Progenitors Anti-MKI67 (Ki67) HPA000451 Polyclonal IHCICC-IF 66 67
TargetCells
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Cytoskeleton Anti-ACTN1 HPA006035 Polyclonal IHCWB 98 99
Cytoskeleton Anti-ACTN4 HPA001873 Polyclonal IHCWBICC-IF 99 98
Cytoskeleton Anti-INA HPA008057 Polyclonal IHC WB ICC-IF 83 84
Cytoskeleton Anti-MAP1A HPA039064 Polyclonal IHC 60 52
Cytoskeleton Anti-MAP1B HPA022275 Polyclonal IHCWB ICC-IF 85 86
Cytoskeleton Anti-MAP2 HPA008273 Polyclonal IHC WB ICC-IF 96 96
Cytoskeleton Anti-MYO5A HPA001356 Polyclonal IHCICC-IF 99 98
Cytoskeleton Anti-NEFM (NF160) HPA022845 Polyclonal IHCWBICC-IF 98 98
Microglia Anti-AIF HPA049234 Polyclonal IHC 84 84
Microglia Anti-CD68 AMAb90874 Monoclonal IHCWB 76 76
Microglia Anti-CD68 HPA048982 Polyclonal IHC WB 76 76
Microglia Anti-ITGAM (CD11b) AMAb90911 Monoclonal IHC WB 67 68
Microglia Anti-PTPRC AMAb90518 Monoclonal IHCWB 35 37
Microglia Anti-PTPRC HPA000440 Polyclonal IHCWB 35 37
Microglia Anti-TMEM119 AMAb91528 Monoclonal IHCWB ICC-IF 52 46
Microglia Anti-TMEM119 HPA051870 Polyclonal IHC 52 46
Neurons Anti-NEFM (NF160) AMAb91027 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91028 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91029 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91030 Monoclonal IHC WB 98 98
Neurons Anti-NEFH (NF200) AMAb91025 Monoclonal IHC WB 98 98
Neurons Anti-NEFH (NF200) HPA061615 Polyclonal IHC 88 94
Neurons Anti-UCHL1 AMAb91145 Monoclonal IHC WB ICC-IF 97 97
Neurons Anti-UCHL1 HPA005993 Polyclonal IHCWBICC-IF 97 97
Neurons Anti-VIM AMAb90516 Monoclonal IHCWB 99 99
Neurons Anti-VIM HPA001762 Polyclonal IHCWBICC-IF 99 99
Schwann cells Anti-MBP AMAb91062 Monoclonal IHC WB ICC-IF 97 97
Schwann cells Anti-MBP AMAb91063 Monoclonal IHC WB ICC-IF 97 97
Schwann cells Anti-MBP AMAb91064 Monoclonal IHC WB 97 97
Anti-I
TM2B antibodyNeurodegenerative diseases such as Amyotrophic Lateral Sclerosis Parkinsonrsquos Alzheimerrsquos and Huntingtonrsquos diseases occur as a result of the progressive loss of structure and function in neurons leading to the death of neurons
Age remains the primary risk factor for almost all the neurodegenerative disorders thus interfering with age-related molecular mechanisms or pathways might be an avenue to develop new therapeutics
The choline acetyltransferase (ChAT) is an enzyme responsible for catalyzing the synthesis of acetylcholine and is expressed in the majority of cholinergic neurons Hence ChAT immunoreactivity is used to detect cognitive decline in several neurodegenerative disorders
Integral membrane protein 2B (ITM2B) is a protein that in humans is encoded by the ITM2B gene This gene located on chromosome 13 is connected to familial dementia causing amyloid and pre-fibrillar effects similar to those seen in Alzheimerrsquos
The expression of the ubiquitin-carboxyl-terminal hydrolase isozyme L1 (UCHL1) present both in the neuronal cells of the central and peripheral nervous system is down-regulated in Parkinsonrsquos and Alzheimerrsquos disease patients
NEURODEGENERATIVE DISEASES
Anti-C
HAT antibody
Anti-U
CHL1 antibody
Anti-CHAT monoclonal antibody (AMAb91130) shows strong immunoreactivity in the
acetylcholine neurons in the rat diagonal band of Broca in green
Anti-ITM2B polyclonal antibody (HPA029292) targeting Integral membrane protein 2B strongly
labels Golgi apparatus in A-431 cells in green
Anti-UCHL1 monoclonal antibody (AMAb91145) in the mouse trigeminal ganglion show strong
immunoreactrivity in primary sensory neurons in green
12
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for NeurodegenerationProduct Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-ADAR AMAb90535 Monoclonal IHCWB ICC-IF 86 85
Anti-ADAR HPA003890 Polyclonal IHCWBICC-IF 86 85
Anti-AIMP1 HPA018476 Polyclonal IHCWBICC-IF 96 97
Anti-AKT1 AMAb90834 Monoclonal WB ICC-IF 97 97
Anti-AOX1 HPA040199 Polyclonal IHC 84 86
Anti-APBA3 HPA045577 Polyclonal IHC 70 68
Anti-APBB2 HPA023542 Polyclonal IHCWBICC-IF 85 83
Anti-APBB3 HPA005571 Polyclonal IHCICC-IF 84 84
Anti-APOA4 AMAb90769 Monoclonal IHC WB 65 28
Anti-APP HPA001462 Polyclonal IHCWB 95 95
Anti-AQP4 AMAb90537 Monoclonal IHCWB 93 92
Anti-AQP4 HPA014784 Polyclonal IHCWB ICC-IF 93 92
Anti-ATF2 HPA022134 Polyclonal IHCWBICC-IF 99 99
Anti-ATF3 AMAb90909 Monoclonal IHCICC-IF 92 92
Anti-ATF3 HPA001562 Polyclonal IHCICC-IF 92 92
Anti-ATRX AMAb90784 Monoclonal IHC WBICC-IF 96 97
Anti-ATXN1 HPA008335 Polyclonal IHC 81 81
Anti-ATXN2 HPA018295 Polyclonal IHCWBICC-IF 90 91
Anti-C3 HPA020432 Polyclonal IHC 78 23
Anti-CASP3 HPA002643 Polyclonal IHCWBICC-IF 84 88
Anti-CD4 AMAb90754 Monoclonal IHCWB 55 57
Anti-CD40 AMAb90905 Monoclonal IHCWB 58 54
Anti-CHAT AMAb91130 Monoclonal IHCICC-IF 96 96
Anti-CHAT HPA048547 Polyclonal IHC 96 96
Anti-CHGA AMAb90525 Monoclonal IHCWBICC-IF 62 64
Anti-COX1 HPA002834 Polyclonal IHCWBICC-IF 93 90
Anti-COX2PTGS2 HPA001335 Polyclonal IHCICC-IF 88 88
Anti-CTSB HPA018156 Polyclonal IHCICC-IF 79 79
Anti-CTSD HPA003001 Polyclonal IHCWB 86 86
Anti-CXorf27 HPA003356 Polyclonal IHC 47 46
Anti-FBXO7 (PARK15) HPA032114 Polyclonal IHC 78 81
Anti-FUS AMAb90549 Monoclonal IHC WB ICC-IF 91 91Anti-GSK3B HPA028017 Polyclonal IHCWBICC-IF 100 100Anti-HIP1 HPA017964 Polyclonal IHCWB 79 77Anti-HTRA2 (PARK13) HPA027366 Polyclonal IHCWB 57 63Anti-ITGAM (CD11b) AMAb90911 Monoclonal IHCWB 67 68
Anti-ITM2B HPA029292 Polyclonal IHC ICC-IF 95 96Anti-LRP2 HPA005980 Polyclonal IHC 78 36
Products with enhanced validation for indicated application (see page 16)
13
Product Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-NFKB1 HPA027305 Polyclonal IHCWBICC-IF 60 62
Anti-OPTN HPA003360 Polyclonal IHCWBICC-IF 64 68
Anti-PADI4 HPA017007 Polyclonal IHCWB 66 69
Anti-PARK7 HPA004190 Polyclonal IHCWB 89 90
Anti-PFN1 AMAb91181 Monoclonal IHCWB 87 87
Anti-PHGDH AMAb90786 Monoclonal IHCWBICC-IF 99 99
Anti-PHGDH HPA021241 Polyclonal IHCWBICC-IF 99 99
Anti-PRNP HPA042754 Polyclonal IHC 91 91
Anti-PSEN1 HPA030760 Polyclonal IHC 82 81
Anti-RHOT1 AMAb90852 Monoclonal IHCWB 100 100
Anti-RHOT1 HPA010687 Polyclonal IHC 100 100
Anti-S100A8 HPA024372 Polyclonal IHCWBICC-IF 56 60
Anti-SERPINA3 HPA002560 Polyclonal IHCWB 60 59
Anti-SNCB HPA035876 Polyclonal IHCWB 97 97
Anti-SOD1 HPA001401 Polyclonal IHCWBICC-IF 82 81
Anti-SOD2 HPA001814 Polyclonal IHCWB 88 87
Anti-THY1 AMAb90844 Monoclonal IHCWB 64 68
Anti-THY1 HPA003733 Polyclonal IHCICC-IF 64 68
Anti-TNFRSF21 HPA006746 Polyclonal IHCWB 86 85
Anti-TTR AMAb90649 Monoclonal IHC WB 81 83
Anti-UBE2K HPA028869 Polyclonal IHCICC-IF 100 100
Anti-UCHL1 HPA005993 Polyclonal IHCWBICC-IF 97 97
Anti-USP46 AMAb90722 Monoclonal WBICC-IF 100 99
Anti-VPS26A AMAb90967 Monoclonal IHC WBICC-IF 96 100
Anti-WHSC1 AMAb90851 Monoclonal IHCWBICC-IF 91 91
Anti-WHSC1 HPA015801 Polyclonal IHCICC-IF 91 91
Anti-VWF AMAb90928 Monoclonal IHCWB 80 80
Anti-VWF HPA002082 Polyclonal IHC 82 78
The development of the central nervous system includes the development of the brain spinal cord optic and auditory systems
Within the neural tube stem cells generate the two major classes of cells that make the majority of the nervous system neurons and glia Both these classes of cells differentiate into many different types generated with highly specialized functions and shapes
The development of the peripheral and central nervous systems begins early in embryogenesis and can be traced through the use of lineage antibodies specific to each stage of neuronal development
The (SOX) family of transcription factors are well-established regulators of cell fate decisions during development
Vimentin (VIM) is a type III intermediate filament protein expressed in mesenchymal cells It is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both healthy development and metastatic progression
The cell surface glycoprotein Thy1 (CD90) is expressed on many mammalian cell types including neurons and serves as a human mesenchymal stem cell (MSC) marker
DEVELOPMENT
A
nti-VIM antibody
A
nti-SOX4 antibody
Anti-T
HY1 antibody
Anti-VIM polyclonal antibody (HPA001762) immunofluorescent staining of human cell line
ASC TERT1 shows localization to intermediate filaments in green
Anti-SOX4 monoclonal antibody (AMAb913802) immunofluorescent staining in mouse
hippocampus showing immunoreactivity in a subset of neurons in the granule cell layer of
the dentate gyrus in brown
Anti-THY1 polyclonal antibody (HPA003733) staining of human cerebral cortex shows
localization to neuropils in brown
Product Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-ENG AMAb90925 Monoclonal IHC 66 22Anti-ENG HPA011862 Polyclonal IHCWB 66 22Anti-FABP7 AMAb90595 Monoclonal IHCWB 89 90Anti-FABP7 HPA028825 Polyclonal IHCWB 89 90Anti-FLT1 AMAb90704 Monoclonal IHCWB 80 82Anti-GAP43 HPA015600 Polyclonal IHC ICC-IF 71 70Anti-GLI3 HPA005534 Polyclonal IHC 74 76Anti-MEF2C AMAb90727 Monoclonal IHCWBICC-IF 97 47Anti-MEF2C HPA005533 Polyclonal IHCWBICC-IF 97 47Anti-MKI67 (Ki67) HPA000451 Polyclonal IHCICC-IF 66 67Anti-NACC1 HPA021238 Polyclonal IHCICC-IF 91 89Anti-NES (Nestin) AMAb90556 Monoclonal IHCWBICC-IF 47 42Anti-NES (Nestin) HPA007007 Polyclonal IHCWB 47 42Anti-NKX2-2 HPA003468 Polyclonal IHCWB 96 97Anti-PAX6 HPA030775 Polyclonal IHCICC-IF 100 100Anti-PBK HPA005753 Polyclonal IHCWBICC-IF 91 94Anti-REST AMAb90740 Monoclonal IHCICC-IF 41 43Anti-RUNX1 HPA004176 Polyclonal IHCICC-IF 93 93Anti-RUNX2 AMAb90591 Monoclonal IHCWBICC-IF 100 81Anti-RUNX2 HPA022040 Polyclonal IHCWBICC-IF 100 81Anti-SATB2 AMAb90679 Monoclonal IHCWBICC-IF 100 100Anti-SATB2 HPA029543 Polyclonal IHCWBICC-IF 100 100Anti-SOX4 HPA029901 Polyclonal IHCICC-IF 100 39Anti-SOX6 HPA001923 Polyclonal IHCICC-IF 96 96Anti-SOX7 HPA009065 Polyclonal IHCWB 91 91Anti-SOX9 AMAb90795 Monoclonal IHCWBICC-IF 97 96Anti-SOX11 AMAb90502 Monoclonal IHCWB 82 82Anti-SOX11 HPA000536 Polyclonal IHCWB 82 82Anti-SOX30 HPA006159 Polyclonal IHCWB 68 70Anti-THY1 AMAb90844 Monoclonal IHCWB 64 68Anti-THY1 HPA003733 Polyclonal IHCICC-IF 64 68Anti-TM4SF2TSPAN7 HPA003140 Polyclonal IHCWB 96 96Anti-TM4SF2TSPAN7 AMAb90621 Monoclonal IHCWB 96 96Anti-VANGL1 AMAb90600 Monoclonal WBICC-IF 95 95Anti-VIM AMAb90516 Monoclonal IHCWB 99 99Anti-VIM HPA001762 Polyclonal IHCWBICC-IF 99 99
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Development
Products with enhanced validation for indicated application (see page 16)
15
At Atlas Antibodies we extensively validate our antibodies in IHC WB and ICC-IF Enhanced Validation is performed as an additional layer of security in an application and context-specific manner Enhanced validation offers increased security of antibody specificity in a defined context This is ensured by using the most relevant validation method for each combination of protein sample and application Enhanced Validation follows the guidelines proposed by the International Working Group for Antibody Validation (IWGAV)
ENHANCED VALIDATION
GENETIC VALIDATION Antibody specificity is confirmed by downregulating the target protein on a genetic level using siRNA
ORTHOGONAL VALIDATIONThe antibody is validated by comparing the results with a non-antibody based method across multiple samples
VALIDATION BY INDEPENDENT ANTIBODIESThe antibody specificity is demonstrated by comparing two antibodies targeting different regions of the same protein
RECOMBINANT EXPRESSION VALIDATIONThe antibody binding is verified using and over-expressed or tagged version of the target protein
MIGRATION CAPTURE MS VALIDATIONThe staining pattern and the protein size detected by the antibody is compared with results obtained by a capture Mass Spectrometry (MS) method
16
Triple A PolyclonalsTM are rabbit polyclonal primary antibodies developed within the Human Protein Atlas project IHC characterization data from 44 normal and 20 cancer tissues is available on the Human Protein Atlas portal
PrecisA MonoclonalsTM are mouse monoclonal primary antibodies developed against a number of carefully selected targets Clones are selected to recognize only unique non-overlapping epitopes and isotypes
Atlas Antibodies ABVoltavaumlgen 13SE-168 69 BrommaSwedenatlasantibodiescom
Phone +46(0)8 54 59 58 50contactatlasantibodiescomorderatlasantibodiescomsupportatlasantibodiescom
Atlas Antibodies logo Triple A Polyclonals PrecisA Monoclonals PrEST Antigen and QPrEST are trademarks or registered trademarks of Atlas Antibodies AB All other trademarks are the property of their respective owners
Products are for research use only Not for use in diagnostic procedures copy Atlas Antibodies AB 2020 008_
Neu
ro M
arke
r Pan
el _
Rev
03_2
0121
5_S
fN E
ditio
n
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Neural Lineage
Products with enhanced validation for indicated application (see page 16)
11
TargetCells
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Astrocytes Anti-GFAP AMAb91033 Monoclonal IHC WB 98 100
Astrocytes Anti-GFAP HPA056030 Polyclonal IHCWB ICC-IF 98 100
Astrocytes Anti-GLUL AMAb91101 Monoclonal IHC WB 95 53
Astrocytes Anti-GLUL AMAb91102 Monoclonal IHC WB 95 92
Astrocytes Anti-GLUL AMAb91103 Monoclonal IHC WB 95 92
Astrocytes Anti-GLUL HPA007316 Polyclonal IHCWB 95 53
Astrocytes Anti-S100B AMAb91038 Monoclonal IHC WB 99 98
Astroglia Anti-EZR AMAb90976 Monoclonal IHCWBICC-IF 93 93
Astroglia Anti-EZR HPA021616 Polyclonal IHCWBICC-IF 93 93
Adhesion molecule Anti-ICAM5 HPA009083 Polyclonal IHC 85 86
Adhesion molecule Anti-MCAM HPA008848 Polyclonal IHC WB 75 73
Adhesion molecule Anti-NCAM2 HPA030900 Polyclonal IHC 89 91
Adhesion molecule Anti-NLGN1 HPA006680 Polyclonal IHCWB 98 98
Calcium binding protein Anti-CALB1 (CB) HPA023099 Polyclonal IHCWBICC-IF 98 99
Calcium binding protein Anti-CALB2 (CR) HPA007305 Polyclonal IHCWBICC-IF 100 100
Calcium binding proteins Anti-NECAB1 AMAb90801 Monoclonal IHCWB 98 98
Calcium binding protein Anti-NECAB1 HPA023629 Polyclonal IHCWB 98 98
Calcium binding protein Anti-NECAB2 AMAb90808 Monoclonal IHC 85 84
Calcium binding protein Anti-NECAB2 HPA013998 Polyclonal IHCICC-IF 98 97
Calcium binding protein Anti-SCGN AMAb90630 Monoclonal IHCWB 96 96
Calcium binding protein Anti-SCGN HPA006641 Polyclonal IHC 96 96
Calcium binding protein Anti-S100B HPA015768 Polyclonal IHCWBICC-IF 99 98
Oligodendrocytes Anti-MOG AMAb91066 Monoclonal IHC WB 91 89
Oligodendrocytes Anti-MOG AMAb91067 Monoclonal IHC WB 91 89
Oligodendrocytes Anti-MOG HPA021873 Polyclonal IHC 91 89
Oligodendrocytes Anti-CNP AMAb91068 Monoclonal IHC WB 76 77
Oligodendrocytes Anti-CNP AMAb91069 Monoclonal IHC WB 76 77
Oligodendrocytes Anti-CNP AMAb91072 Monoclonal IHC WB ICC-IF 76 77
Oligodendrocytes Anti-CNP HPA023280 Polyclonal IHCWBICC-IF 76 77
Progenitors Anti-PBK HPA005753 Polyclonal IHCWBICC-IF 91 94
Progenitors Anti-MKI67 (Ki67) AMAb90870 Monoclonal IHC ICC-IF 68 68
Progenitors Anti-MKI67 (Ki67) HPA000451 Polyclonal IHCICC-IF 66 67
TargetCells
Product Name
Product Number Clonality Validated
ApplicationsSeq Identity mouse rat
Cytoskeleton Anti-ACTN1 HPA006035 Polyclonal IHCWB 98 99
Cytoskeleton Anti-ACTN4 HPA001873 Polyclonal IHCWBICC-IF 99 98
Cytoskeleton Anti-INA HPA008057 Polyclonal IHC WB ICC-IF 83 84
Cytoskeleton Anti-MAP1A HPA039064 Polyclonal IHC 60 52
Cytoskeleton Anti-MAP1B HPA022275 Polyclonal IHCWB ICC-IF 85 86
Cytoskeleton Anti-MAP2 HPA008273 Polyclonal IHC WB ICC-IF 96 96
Cytoskeleton Anti-MYO5A HPA001356 Polyclonal IHCICC-IF 99 98
Cytoskeleton Anti-NEFM (NF160) HPA022845 Polyclonal IHCWBICC-IF 98 98
Microglia Anti-AIF HPA049234 Polyclonal IHC 84 84
Microglia Anti-CD68 AMAb90874 Monoclonal IHCWB 76 76
Microglia Anti-CD68 HPA048982 Polyclonal IHC WB 76 76
Microglia Anti-ITGAM (CD11b) AMAb90911 Monoclonal IHC WB 67 68
Microglia Anti-PTPRC AMAb90518 Monoclonal IHCWB 35 37
Microglia Anti-PTPRC HPA000440 Polyclonal IHCWB 35 37
Microglia Anti-TMEM119 AMAb91528 Monoclonal IHCWB ICC-IF 52 46
Microglia Anti-TMEM119 HPA051870 Polyclonal IHC 52 46
Neurons Anti-NEFM (NF160) AMAb91027 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91028 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91029 Monoclonal IHC WB 98 98
Neurons Anti-NEFM (NF160) AMAb91030 Monoclonal IHC WB 98 98
Neurons Anti-NEFH (NF200) AMAb91025 Monoclonal IHC WB 98 98
Neurons Anti-NEFH (NF200) HPA061615 Polyclonal IHC 88 94
Neurons Anti-UCHL1 AMAb91145 Monoclonal IHC WB ICC-IF 97 97
Neurons Anti-UCHL1 HPA005993 Polyclonal IHCWBICC-IF 97 97
Neurons Anti-VIM AMAb90516 Monoclonal IHCWB 99 99
Neurons Anti-VIM HPA001762 Polyclonal IHCWBICC-IF 99 99
Schwann cells Anti-MBP AMAb91062 Monoclonal IHC WB ICC-IF 97 97
Schwann cells Anti-MBP AMAb91063 Monoclonal IHC WB ICC-IF 97 97
Schwann cells Anti-MBP AMAb91064 Monoclonal IHC WB 97 97
Anti-I
TM2B antibodyNeurodegenerative diseases such as Amyotrophic Lateral Sclerosis Parkinsonrsquos Alzheimerrsquos and Huntingtonrsquos diseases occur as a result of the progressive loss of structure and function in neurons leading to the death of neurons
Age remains the primary risk factor for almost all the neurodegenerative disorders thus interfering with age-related molecular mechanisms or pathways might be an avenue to develop new therapeutics
The choline acetyltransferase (ChAT) is an enzyme responsible for catalyzing the synthesis of acetylcholine and is expressed in the majority of cholinergic neurons Hence ChAT immunoreactivity is used to detect cognitive decline in several neurodegenerative disorders
Integral membrane protein 2B (ITM2B) is a protein that in humans is encoded by the ITM2B gene This gene located on chromosome 13 is connected to familial dementia causing amyloid and pre-fibrillar effects similar to those seen in Alzheimerrsquos
The expression of the ubiquitin-carboxyl-terminal hydrolase isozyme L1 (UCHL1) present both in the neuronal cells of the central and peripheral nervous system is down-regulated in Parkinsonrsquos and Alzheimerrsquos disease patients
NEURODEGENERATIVE DISEASES
Anti-C
HAT antibody
Anti-U
CHL1 antibody
Anti-CHAT monoclonal antibody (AMAb91130) shows strong immunoreactivity in the
acetylcholine neurons in the rat diagonal band of Broca in green
Anti-ITM2B polyclonal antibody (HPA029292) targeting Integral membrane protein 2B strongly
labels Golgi apparatus in A-431 cells in green
Anti-UCHL1 monoclonal antibody (AMAb91145) in the mouse trigeminal ganglion show strong
immunoreactrivity in primary sensory neurons in green
12
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for NeurodegenerationProduct Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-ADAR AMAb90535 Monoclonal IHCWB ICC-IF 86 85
Anti-ADAR HPA003890 Polyclonal IHCWBICC-IF 86 85
Anti-AIMP1 HPA018476 Polyclonal IHCWBICC-IF 96 97
Anti-AKT1 AMAb90834 Monoclonal WB ICC-IF 97 97
Anti-AOX1 HPA040199 Polyclonal IHC 84 86
Anti-APBA3 HPA045577 Polyclonal IHC 70 68
Anti-APBB2 HPA023542 Polyclonal IHCWBICC-IF 85 83
Anti-APBB3 HPA005571 Polyclonal IHCICC-IF 84 84
Anti-APOA4 AMAb90769 Monoclonal IHC WB 65 28
Anti-APP HPA001462 Polyclonal IHCWB 95 95
Anti-AQP4 AMAb90537 Monoclonal IHCWB 93 92
Anti-AQP4 HPA014784 Polyclonal IHCWB ICC-IF 93 92
Anti-ATF2 HPA022134 Polyclonal IHCWBICC-IF 99 99
Anti-ATF3 AMAb90909 Monoclonal IHCICC-IF 92 92
Anti-ATF3 HPA001562 Polyclonal IHCICC-IF 92 92
Anti-ATRX AMAb90784 Monoclonal IHC WBICC-IF 96 97
Anti-ATXN1 HPA008335 Polyclonal IHC 81 81
Anti-ATXN2 HPA018295 Polyclonal IHCWBICC-IF 90 91
Anti-C3 HPA020432 Polyclonal IHC 78 23
Anti-CASP3 HPA002643 Polyclonal IHCWBICC-IF 84 88
Anti-CD4 AMAb90754 Monoclonal IHCWB 55 57
Anti-CD40 AMAb90905 Monoclonal IHCWB 58 54
Anti-CHAT AMAb91130 Monoclonal IHCICC-IF 96 96
Anti-CHAT HPA048547 Polyclonal IHC 96 96
Anti-CHGA AMAb90525 Monoclonal IHCWBICC-IF 62 64
Anti-COX1 HPA002834 Polyclonal IHCWBICC-IF 93 90
Anti-COX2PTGS2 HPA001335 Polyclonal IHCICC-IF 88 88
Anti-CTSB HPA018156 Polyclonal IHCICC-IF 79 79
Anti-CTSD HPA003001 Polyclonal IHCWB 86 86
Anti-CXorf27 HPA003356 Polyclonal IHC 47 46
Anti-FBXO7 (PARK15) HPA032114 Polyclonal IHC 78 81
Anti-FUS AMAb90549 Monoclonal IHC WB ICC-IF 91 91Anti-GSK3B HPA028017 Polyclonal IHCWBICC-IF 100 100Anti-HIP1 HPA017964 Polyclonal IHCWB 79 77Anti-HTRA2 (PARK13) HPA027366 Polyclonal IHCWB 57 63Anti-ITGAM (CD11b) AMAb90911 Monoclonal IHCWB 67 68
Anti-ITM2B HPA029292 Polyclonal IHC ICC-IF 95 96Anti-LRP2 HPA005980 Polyclonal IHC 78 36
Products with enhanced validation for indicated application (see page 16)
13
Product Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-NFKB1 HPA027305 Polyclonal IHCWBICC-IF 60 62
Anti-OPTN HPA003360 Polyclonal IHCWBICC-IF 64 68
Anti-PADI4 HPA017007 Polyclonal IHCWB 66 69
Anti-PARK7 HPA004190 Polyclonal IHCWB 89 90
Anti-PFN1 AMAb91181 Monoclonal IHCWB 87 87
Anti-PHGDH AMAb90786 Monoclonal IHCWBICC-IF 99 99
Anti-PHGDH HPA021241 Polyclonal IHCWBICC-IF 99 99
Anti-PRNP HPA042754 Polyclonal IHC 91 91
Anti-PSEN1 HPA030760 Polyclonal IHC 82 81
Anti-RHOT1 AMAb90852 Monoclonal IHCWB 100 100
Anti-RHOT1 HPA010687 Polyclonal IHC 100 100
Anti-S100A8 HPA024372 Polyclonal IHCWBICC-IF 56 60
Anti-SERPINA3 HPA002560 Polyclonal IHCWB 60 59
Anti-SNCB HPA035876 Polyclonal IHCWB 97 97
Anti-SOD1 HPA001401 Polyclonal IHCWBICC-IF 82 81
Anti-SOD2 HPA001814 Polyclonal IHCWB 88 87
Anti-THY1 AMAb90844 Monoclonal IHCWB 64 68
Anti-THY1 HPA003733 Polyclonal IHCICC-IF 64 68
Anti-TNFRSF21 HPA006746 Polyclonal IHCWB 86 85
Anti-TTR AMAb90649 Monoclonal IHC WB 81 83
Anti-UBE2K HPA028869 Polyclonal IHCICC-IF 100 100
Anti-UCHL1 HPA005993 Polyclonal IHCWBICC-IF 97 97
Anti-USP46 AMAb90722 Monoclonal WBICC-IF 100 99
Anti-VPS26A AMAb90967 Monoclonal IHC WBICC-IF 96 100
Anti-WHSC1 AMAb90851 Monoclonal IHCWBICC-IF 91 91
Anti-WHSC1 HPA015801 Polyclonal IHCICC-IF 91 91
Anti-VWF AMAb90928 Monoclonal IHCWB 80 80
Anti-VWF HPA002082 Polyclonal IHC 82 78
The development of the central nervous system includes the development of the brain spinal cord optic and auditory systems
Within the neural tube stem cells generate the two major classes of cells that make the majority of the nervous system neurons and glia Both these classes of cells differentiate into many different types generated with highly specialized functions and shapes
The development of the peripheral and central nervous systems begins early in embryogenesis and can be traced through the use of lineage antibodies specific to each stage of neuronal development
The (SOX) family of transcription factors are well-established regulators of cell fate decisions during development
Vimentin (VIM) is a type III intermediate filament protein expressed in mesenchymal cells It is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both healthy development and metastatic progression
The cell surface glycoprotein Thy1 (CD90) is expressed on many mammalian cell types including neurons and serves as a human mesenchymal stem cell (MSC) marker
DEVELOPMENT
A
nti-VIM antibody
A
nti-SOX4 antibody
Anti-T
HY1 antibody
Anti-VIM polyclonal antibody (HPA001762) immunofluorescent staining of human cell line
ASC TERT1 shows localization to intermediate filaments in green
Anti-SOX4 monoclonal antibody (AMAb913802) immunofluorescent staining in mouse
hippocampus showing immunoreactivity in a subset of neurons in the granule cell layer of
the dentate gyrus in brown
Anti-THY1 polyclonal antibody (HPA003733) staining of human cerebral cortex shows
localization to neuropils in brown
Product Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-ENG AMAb90925 Monoclonal IHC 66 22Anti-ENG HPA011862 Polyclonal IHCWB 66 22Anti-FABP7 AMAb90595 Monoclonal IHCWB 89 90Anti-FABP7 HPA028825 Polyclonal IHCWB 89 90Anti-FLT1 AMAb90704 Monoclonal IHCWB 80 82Anti-GAP43 HPA015600 Polyclonal IHC ICC-IF 71 70Anti-GLI3 HPA005534 Polyclonal IHC 74 76Anti-MEF2C AMAb90727 Monoclonal IHCWBICC-IF 97 47Anti-MEF2C HPA005533 Polyclonal IHCWBICC-IF 97 47Anti-MKI67 (Ki67) HPA000451 Polyclonal IHCICC-IF 66 67Anti-NACC1 HPA021238 Polyclonal IHCICC-IF 91 89Anti-NES (Nestin) AMAb90556 Monoclonal IHCWBICC-IF 47 42Anti-NES (Nestin) HPA007007 Polyclonal IHCWB 47 42Anti-NKX2-2 HPA003468 Polyclonal IHCWB 96 97Anti-PAX6 HPA030775 Polyclonal IHCICC-IF 100 100Anti-PBK HPA005753 Polyclonal IHCWBICC-IF 91 94Anti-REST AMAb90740 Monoclonal IHCICC-IF 41 43Anti-RUNX1 HPA004176 Polyclonal IHCICC-IF 93 93Anti-RUNX2 AMAb90591 Monoclonal IHCWBICC-IF 100 81Anti-RUNX2 HPA022040 Polyclonal IHCWBICC-IF 100 81Anti-SATB2 AMAb90679 Monoclonal IHCWBICC-IF 100 100Anti-SATB2 HPA029543 Polyclonal IHCWBICC-IF 100 100Anti-SOX4 HPA029901 Polyclonal IHCICC-IF 100 39Anti-SOX6 HPA001923 Polyclonal IHCICC-IF 96 96Anti-SOX7 HPA009065 Polyclonal IHCWB 91 91Anti-SOX9 AMAb90795 Monoclonal IHCWBICC-IF 97 96Anti-SOX11 AMAb90502 Monoclonal IHCWB 82 82Anti-SOX11 HPA000536 Polyclonal IHCWB 82 82Anti-SOX30 HPA006159 Polyclonal IHCWB 68 70Anti-THY1 AMAb90844 Monoclonal IHCWB 64 68Anti-THY1 HPA003733 Polyclonal IHCICC-IF 64 68Anti-TM4SF2TSPAN7 HPA003140 Polyclonal IHCWB 96 96Anti-TM4SF2TSPAN7 AMAb90621 Monoclonal IHCWB 96 96Anti-VANGL1 AMAb90600 Monoclonal WBICC-IF 95 95Anti-VIM AMAb90516 Monoclonal IHCWB 99 99Anti-VIM HPA001762 Polyclonal IHCWBICC-IF 99 99
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Development
Products with enhanced validation for indicated application (see page 16)
15
At Atlas Antibodies we extensively validate our antibodies in IHC WB and ICC-IF Enhanced Validation is performed as an additional layer of security in an application and context-specific manner Enhanced validation offers increased security of antibody specificity in a defined context This is ensured by using the most relevant validation method for each combination of protein sample and application Enhanced Validation follows the guidelines proposed by the International Working Group for Antibody Validation (IWGAV)
ENHANCED VALIDATION
GENETIC VALIDATION Antibody specificity is confirmed by downregulating the target protein on a genetic level using siRNA
ORTHOGONAL VALIDATIONThe antibody is validated by comparing the results with a non-antibody based method across multiple samples
VALIDATION BY INDEPENDENT ANTIBODIESThe antibody specificity is demonstrated by comparing two antibodies targeting different regions of the same protein
RECOMBINANT EXPRESSION VALIDATIONThe antibody binding is verified using and over-expressed or tagged version of the target protein
MIGRATION CAPTURE MS VALIDATIONThe staining pattern and the protein size detected by the antibody is compared with results obtained by a capture Mass Spectrometry (MS) method
16
Triple A PolyclonalsTM are rabbit polyclonal primary antibodies developed within the Human Protein Atlas project IHC characterization data from 44 normal and 20 cancer tissues is available on the Human Protein Atlas portal
PrecisA MonoclonalsTM are mouse monoclonal primary antibodies developed against a number of carefully selected targets Clones are selected to recognize only unique non-overlapping epitopes and isotypes
Atlas Antibodies ABVoltavaumlgen 13SE-168 69 BrommaSwedenatlasantibodiescom
Phone +46(0)8 54 59 58 50contactatlasantibodiescomorderatlasantibodiescomsupportatlasantibodiescom
Atlas Antibodies logo Triple A Polyclonals PrecisA Monoclonals PrEST Antigen and QPrEST are trademarks or registered trademarks of Atlas Antibodies AB All other trademarks are the property of their respective owners
Products are for research use only Not for use in diagnostic procedures copy Atlas Antibodies AB 2020 008_
Neu
ro M
arke
r Pan
el _
Rev
03_2
0121
5_S
fN E
ditio
n
Anti-I
TM2B antibodyNeurodegenerative diseases such as Amyotrophic Lateral Sclerosis Parkinsonrsquos Alzheimerrsquos and Huntingtonrsquos diseases occur as a result of the progressive loss of structure and function in neurons leading to the death of neurons
Age remains the primary risk factor for almost all the neurodegenerative disorders thus interfering with age-related molecular mechanisms or pathways might be an avenue to develop new therapeutics
The choline acetyltransferase (ChAT) is an enzyme responsible for catalyzing the synthesis of acetylcholine and is expressed in the majority of cholinergic neurons Hence ChAT immunoreactivity is used to detect cognitive decline in several neurodegenerative disorders
Integral membrane protein 2B (ITM2B) is a protein that in humans is encoded by the ITM2B gene This gene located on chromosome 13 is connected to familial dementia causing amyloid and pre-fibrillar effects similar to those seen in Alzheimerrsquos
The expression of the ubiquitin-carboxyl-terminal hydrolase isozyme L1 (UCHL1) present both in the neuronal cells of the central and peripheral nervous system is down-regulated in Parkinsonrsquos and Alzheimerrsquos disease patients
NEURODEGENERATIVE DISEASES
Anti-C
HAT antibody
Anti-U
CHL1 antibody
Anti-CHAT monoclonal antibody (AMAb91130) shows strong immunoreactivity in the
acetylcholine neurons in the rat diagonal band of Broca in green
Anti-ITM2B polyclonal antibody (HPA029292) targeting Integral membrane protein 2B strongly
labels Golgi apparatus in A-431 cells in green
Anti-UCHL1 monoclonal antibody (AMAb91145) in the mouse trigeminal ganglion show strong
immunoreactrivity in primary sensory neurons in green
12
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for NeurodegenerationProduct Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-ADAR AMAb90535 Monoclonal IHCWB ICC-IF 86 85
Anti-ADAR HPA003890 Polyclonal IHCWBICC-IF 86 85
Anti-AIMP1 HPA018476 Polyclonal IHCWBICC-IF 96 97
Anti-AKT1 AMAb90834 Monoclonal WB ICC-IF 97 97
Anti-AOX1 HPA040199 Polyclonal IHC 84 86
Anti-APBA3 HPA045577 Polyclonal IHC 70 68
Anti-APBB2 HPA023542 Polyclonal IHCWBICC-IF 85 83
Anti-APBB3 HPA005571 Polyclonal IHCICC-IF 84 84
Anti-APOA4 AMAb90769 Monoclonal IHC WB 65 28
Anti-APP HPA001462 Polyclonal IHCWB 95 95
Anti-AQP4 AMAb90537 Monoclonal IHCWB 93 92
Anti-AQP4 HPA014784 Polyclonal IHCWB ICC-IF 93 92
Anti-ATF2 HPA022134 Polyclonal IHCWBICC-IF 99 99
Anti-ATF3 AMAb90909 Monoclonal IHCICC-IF 92 92
Anti-ATF3 HPA001562 Polyclonal IHCICC-IF 92 92
Anti-ATRX AMAb90784 Monoclonal IHC WBICC-IF 96 97
Anti-ATXN1 HPA008335 Polyclonal IHC 81 81
Anti-ATXN2 HPA018295 Polyclonal IHCWBICC-IF 90 91
Anti-C3 HPA020432 Polyclonal IHC 78 23
Anti-CASP3 HPA002643 Polyclonal IHCWBICC-IF 84 88
Anti-CD4 AMAb90754 Monoclonal IHCWB 55 57
Anti-CD40 AMAb90905 Monoclonal IHCWB 58 54
Anti-CHAT AMAb91130 Monoclonal IHCICC-IF 96 96
Anti-CHAT HPA048547 Polyclonal IHC 96 96
Anti-CHGA AMAb90525 Monoclonal IHCWBICC-IF 62 64
Anti-COX1 HPA002834 Polyclonal IHCWBICC-IF 93 90
Anti-COX2PTGS2 HPA001335 Polyclonal IHCICC-IF 88 88
Anti-CTSB HPA018156 Polyclonal IHCICC-IF 79 79
Anti-CTSD HPA003001 Polyclonal IHCWB 86 86
Anti-CXorf27 HPA003356 Polyclonal IHC 47 46
Anti-FBXO7 (PARK15) HPA032114 Polyclonal IHC 78 81
Anti-FUS AMAb90549 Monoclonal IHC WB ICC-IF 91 91Anti-GSK3B HPA028017 Polyclonal IHCWBICC-IF 100 100Anti-HIP1 HPA017964 Polyclonal IHCWB 79 77Anti-HTRA2 (PARK13) HPA027366 Polyclonal IHCWB 57 63Anti-ITGAM (CD11b) AMAb90911 Monoclonal IHCWB 67 68
Anti-ITM2B HPA029292 Polyclonal IHC ICC-IF 95 96Anti-LRP2 HPA005980 Polyclonal IHC 78 36
Products with enhanced validation for indicated application (see page 16)
13
Product Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-NFKB1 HPA027305 Polyclonal IHCWBICC-IF 60 62
Anti-OPTN HPA003360 Polyclonal IHCWBICC-IF 64 68
Anti-PADI4 HPA017007 Polyclonal IHCWB 66 69
Anti-PARK7 HPA004190 Polyclonal IHCWB 89 90
Anti-PFN1 AMAb91181 Monoclonal IHCWB 87 87
Anti-PHGDH AMAb90786 Monoclonal IHCWBICC-IF 99 99
Anti-PHGDH HPA021241 Polyclonal IHCWBICC-IF 99 99
Anti-PRNP HPA042754 Polyclonal IHC 91 91
Anti-PSEN1 HPA030760 Polyclonal IHC 82 81
Anti-RHOT1 AMAb90852 Monoclonal IHCWB 100 100
Anti-RHOT1 HPA010687 Polyclonal IHC 100 100
Anti-S100A8 HPA024372 Polyclonal IHCWBICC-IF 56 60
Anti-SERPINA3 HPA002560 Polyclonal IHCWB 60 59
Anti-SNCB HPA035876 Polyclonal IHCWB 97 97
Anti-SOD1 HPA001401 Polyclonal IHCWBICC-IF 82 81
Anti-SOD2 HPA001814 Polyclonal IHCWB 88 87
Anti-THY1 AMAb90844 Monoclonal IHCWB 64 68
Anti-THY1 HPA003733 Polyclonal IHCICC-IF 64 68
Anti-TNFRSF21 HPA006746 Polyclonal IHCWB 86 85
Anti-TTR AMAb90649 Monoclonal IHC WB 81 83
Anti-UBE2K HPA028869 Polyclonal IHCICC-IF 100 100
Anti-UCHL1 HPA005993 Polyclonal IHCWBICC-IF 97 97
Anti-USP46 AMAb90722 Monoclonal WBICC-IF 100 99
Anti-VPS26A AMAb90967 Monoclonal IHC WBICC-IF 96 100
Anti-WHSC1 AMAb90851 Monoclonal IHCWBICC-IF 91 91
Anti-WHSC1 HPA015801 Polyclonal IHCICC-IF 91 91
Anti-VWF AMAb90928 Monoclonal IHCWB 80 80
Anti-VWF HPA002082 Polyclonal IHC 82 78
The development of the central nervous system includes the development of the brain spinal cord optic and auditory systems
Within the neural tube stem cells generate the two major classes of cells that make the majority of the nervous system neurons and glia Both these classes of cells differentiate into many different types generated with highly specialized functions and shapes
The development of the peripheral and central nervous systems begins early in embryogenesis and can be traced through the use of lineage antibodies specific to each stage of neuronal development
The (SOX) family of transcription factors are well-established regulators of cell fate decisions during development
Vimentin (VIM) is a type III intermediate filament protein expressed in mesenchymal cells It is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both healthy development and metastatic progression
The cell surface glycoprotein Thy1 (CD90) is expressed on many mammalian cell types including neurons and serves as a human mesenchymal stem cell (MSC) marker
DEVELOPMENT
A
nti-VIM antibody
A
nti-SOX4 antibody
Anti-T
HY1 antibody
Anti-VIM polyclonal antibody (HPA001762) immunofluorescent staining of human cell line
ASC TERT1 shows localization to intermediate filaments in green
Anti-SOX4 monoclonal antibody (AMAb913802) immunofluorescent staining in mouse
hippocampus showing immunoreactivity in a subset of neurons in the granule cell layer of
the dentate gyrus in brown
Anti-THY1 polyclonal antibody (HPA003733) staining of human cerebral cortex shows
localization to neuropils in brown
Product Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-ENG AMAb90925 Monoclonal IHC 66 22Anti-ENG HPA011862 Polyclonal IHCWB 66 22Anti-FABP7 AMAb90595 Monoclonal IHCWB 89 90Anti-FABP7 HPA028825 Polyclonal IHCWB 89 90Anti-FLT1 AMAb90704 Monoclonal IHCWB 80 82Anti-GAP43 HPA015600 Polyclonal IHC ICC-IF 71 70Anti-GLI3 HPA005534 Polyclonal IHC 74 76Anti-MEF2C AMAb90727 Monoclonal IHCWBICC-IF 97 47Anti-MEF2C HPA005533 Polyclonal IHCWBICC-IF 97 47Anti-MKI67 (Ki67) HPA000451 Polyclonal IHCICC-IF 66 67Anti-NACC1 HPA021238 Polyclonal IHCICC-IF 91 89Anti-NES (Nestin) AMAb90556 Monoclonal IHCWBICC-IF 47 42Anti-NES (Nestin) HPA007007 Polyclonal IHCWB 47 42Anti-NKX2-2 HPA003468 Polyclonal IHCWB 96 97Anti-PAX6 HPA030775 Polyclonal IHCICC-IF 100 100Anti-PBK HPA005753 Polyclonal IHCWBICC-IF 91 94Anti-REST AMAb90740 Monoclonal IHCICC-IF 41 43Anti-RUNX1 HPA004176 Polyclonal IHCICC-IF 93 93Anti-RUNX2 AMAb90591 Monoclonal IHCWBICC-IF 100 81Anti-RUNX2 HPA022040 Polyclonal IHCWBICC-IF 100 81Anti-SATB2 AMAb90679 Monoclonal IHCWBICC-IF 100 100Anti-SATB2 HPA029543 Polyclonal IHCWBICC-IF 100 100Anti-SOX4 HPA029901 Polyclonal IHCICC-IF 100 39Anti-SOX6 HPA001923 Polyclonal IHCICC-IF 96 96Anti-SOX7 HPA009065 Polyclonal IHCWB 91 91Anti-SOX9 AMAb90795 Monoclonal IHCWBICC-IF 97 96Anti-SOX11 AMAb90502 Monoclonal IHCWB 82 82Anti-SOX11 HPA000536 Polyclonal IHCWB 82 82Anti-SOX30 HPA006159 Polyclonal IHCWB 68 70Anti-THY1 AMAb90844 Monoclonal IHCWB 64 68Anti-THY1 HPA003733 Polyclonal IHCICC-IF 64 68Anti-TM4SF2TSPAN7 HPA003140 Polyclonal IHCWB 96 96Anti-TM4SF2TSPAN7 AMAb90621 Monoclonal IHCWB 96 96Anti-VANGL1 AMAb90600 Monoclonal WBICC-IF 95 95Anti-VIM AMAb90516 Monoclonal IHCWB 99 99Anti-VIM HPA001762 Polyclonal IHCWBICC-IF 99 99
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Development
Products with enhanced validation for indicated application (see page 16)
15
At Atlas Antibodies we extensively validate our antibodies in IHC WB and ICC-IF Enhanced Validation is performed as an additional layer of security in an application and context-specific manner Enhanced validation offers increased security of antibody specificity in a defined context This is ensured by using the most relevant validation method for each combination of protein sample and application Enhanced Validation follows the guidelines proposed by the International Working Group for Antibody Validation (IWGAV)
ENHANCED VALIDATION
GENETIC VALIDATION Antibody specificity is confirmed by downregulating the target protein on a genetic level using siRNA
ORTHOGONAL VALIDATIONThe antibody is validated by comparing the results with a non-antibody based method across multiple samples
VALIDATION BY INDEPENDENT ANTIBODIESThe antibody specificity is demonstrated by comparing two antibodies targeting different regions of the same protein
RECOMBINANT EXPRESSION VALIDATIONThe antibody binding is verified using and over-expressed or tagged version of the target protein
MIGRATION CAPTURE MS VALIDATIONThe staining pattern and the protein size detected by the antibody is compared with results obtained by a capture Mass Spectrometry (MS) method
16
Triple A PolyclonalsTM are rabbit polyclonal primary antibodies developed within the Human Protein Atlas project IHC characterization data from 44 normal and 20 cancer tissues is available on the Human Protein Atlas portal
PrecisA MonoclonalsTM are mouse monoclonal primary antibodies developed against a number of carefully selected targets Clones are selected to recognize only unique non-overlapping epitopes and isotypes
Atlas Antibodies ABVoltavaumlgen 13SE-168 69 BrommaSwedenatlasantibodiescom
Phone +46(0)8 54 59 58 50contactatlasantibodiescomorderatlasantibodiescomsupportatlasantibodiescom
Atlas Antibodies logo Triple A Polyclonals PrecisA Monoclonals PrEST Antigen and QPrEST are trademarks or registered trademarks of Atlas Antibodies AB All other trademarks are the property of their respective owners
Products are for research use only Not for use in diagnostic procedures copy Atlas Antibodies AB 2020 008_
Neu
ro M
arke
r Pan
el _
Rev
03_2
0121
5_S
fN E
ditio
n
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for NeurodegenerationProduct Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-ADAR AMAb90535 Monoclonal IHCWB ICC-IF 86 85
Anti-ADAR HPA003890 Polyclonal IHCWBICC-IF 86 85
Anti-AIMP1 HPA018476 Polyclonal IHCWBICC-IF 96 97
Anti-AKT1 AMAb90834 Monoclonal WB ICC-IF 97 97
Anti-AOX1 HPA040199 Polyclonal IHC 84 86
Anti-APBA3 HPA045577 Polyclonal IHC 70 68
Anti-APBB2 HPA023542 Polyclonal IHCWBICC-IF 85 83
Anti-APBB3 HPA005571 Polyclonal IHCICC-IF 84 84
Anti-APOA4 AMAb90769 Monoclonal IHC WB 65 28
Anti-APP HPA001462 Polyclonal IHCWB 95 95
Anti-AQP4 AMAb90537 Monoclonal IHCWB 93 92
Anti-AQP4 HPA014784 Polyclonal IHCWB ICC-IF 93 92
Anti-ATF2 HPA022134 Polyclonal IHCWBICC-IF 99 99
Anti-ATF3 AMAb90909 Monoclonal IHCICC-IF 92 92
Anti-ATF3 HPA001562 Polyclonal IHCICC-IF 92 92
Anti-ATRX AMAb90784 Monoclonal IHC WBICC-IF 96 97
Anti-ATXN1 HPA008335 Polyclonal IHC 81 81
Anti-ATXN2 HPA018295 Polyclonal IHCWBICC-IF 90 91
Anti-C3 HPA020432 Polyclonal IHC 78 23
Anti-CASP3 HPA002643 Polyclonal IHCWBICC-IF 84 88
Anti-CD4 AMAb90754 Monoclonal IHCWB 55 57
Anti-CD40 AMAb90905 Monoclonal IHCWB 58 54
Anti-CHAT AMAb91130 Monoclonal IHCICC-IF 96 96
Anti-CHAT HPA048547 Polyclonal IHC 96 96
Anti-CHGA AMAb90525 Monoclonal IHCWBICC-IF 62 64
Anti-COX1 HPA002834 Polyclonal IHCWBICC-IF 93 90
Anti-COX2PTGS2 HPA001335 Polyclonal IHCICC-IF 88 88
Anti-CTSB HPA018156 Polyclonal IHCICC-IF 79 79
Anti-CTSD HPA003001 Polyclonal IHCWB 86 86
Anti-CXorf27 HPA003356 Polyclonal IHC 47 46
Anti-FBXO7 (PARK15) HPA032114 Polyclonal IHC 78 81
Anti-FUS AMAb90549 Monoclonal IHC WB ICC-IF 91 91Anti-GSK3B HPA028017 Polyclonal IHCWBICC-IF 100 100Anti-HIP1 HPA017964 Polyclonal IHCWB 79 77Anti-HTRA2 (PARK13) HPA027366 Polyclonal IHCWB 57 63Anti-ITGAM (CD11b) AMAb90911 Monoclonal IHCWB 67 68
Anti-ITM2B HPA029292 Polyclonal IHC ICC-IF 95 96Anti-LRP2 HPA005980 Polyclonal IHC 78 36
Products with enhanced validation for indicated application (see page 16)
13
Product Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-NFKB1 HPA027305 Polyclonal IHCWBICC-IF 60 62
Anti-OPTN HPA003360 Polyclonal IHCWBICC-IF 64 68
Anti-PADI4 HPA017007 Polyclonal IHCWB 66 69
Anti-PARK7 HPA004190 Polyclonal IHCWB 89 90
Anti-PFN1 AMAb91181 Monoclonal IHCWB 87 87
Anti-PHGDH AMAb90786 Monoclonal IHCWBICC-IF 99 99
Anti-PHGDH HPA021241 Polyclonal IHCWBICC-IF 99 99
Anti-PRNP HPA042754 Polyclonal IHC 91 91
Anti-PSEN1 HPA030760 Polyclonal IHC 82 81
Anti-RHOT1 AMAb90852 Monoclonal IHCWB 100 100
Anti-RHOT1 HPA010687 Polyclonal IHC 100 100
Anti-S100A8 HPA024372 Polyclonal IHCWBICC-IF 56 60
Anti-SERPINA3 HPA002560 Polyclonal IHCWB 60 59
Anti-SNCB HPA035876 Polyclonal IHCWB 97 97
Anti-SOD1 HPA001401 Polyclonal IHCWBICC-IF 82 81
Anti-SOD2 HPA001814 Polyclonal IHCWB 88 87
Anti-THY1 AMAb90844 Monoclonal IHCWB 64 68
Anti-THY1 HPA003733 Polyclonal IHCICC-IF 64 68
Anti-TNFRSF21 HPA006746 Polyclonal IHCWB 86 85
Anti-TTR AMAb90649 Monoclonal IHC WB 81 83
Anti-UBE2K HPA028869 Polyclonal IHCICC-IF 100 100
Anti-UCHL1 HPA005993 Polyclonal IHCWBICC-IF 97 97
Anti-USP46 AMAb90722 Monoclonal WBICC-IF 100 99
Anti-VPS26A AMAb90967 Monoclonal IHC WBICC-IF 96 100
Anti-WHSC1 AMAb90851 Monoclonal IHCWBICC-IF 91 91
Anti-WHSC1 HPA015801 Polyclonal IHCICC-IF 91 91
Anti-VWF AMAb90928 Monoclonal IHCWB 80 80
Anti-VWF HPA002082 Polyclonal IHC 82 78
The development of the central nervous system includes the development of the brain spinal cord optic and auditory systems
Within the neural tube stem cells generate the two major classes of cells that make the majority of the nervous system neurons and glia Both these classes of cells differentiate into many different types generated with highly specialized functions and shapes
The development of the peripheral and central nervous systems begins early in embryogenesis and can be traced through the use of lineage antibodies specific to each stage of neuronal development
The (SOX) family of transcription factors are well-established regulators of cell fate decisions during development
Vimentin (VIM) is a type III intermediate filament protein expressed in mesenchymal cells It is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both healthy development and metastatic progression
The cell surface glycoprotein Thy1 (CD90) is expressed on many mammalian cell types including neurons and serves as a human mesenchymal stem cell (MSC) marker
DEVELOPMENT
A
nti-VIM antibody
A
nti-SOX4 antibody
Anti-T
HY1 antibody
Anti-VIM polyclonal antibody (HPA001762) immunofluorescent staining of human cell line
ASC TERT1 shows localization to intermediate filaments in green
Anti-SOX4 monoclonal antibody (AMAb913802) immunofluorescent staining in mouse
hippocampus showing immunoreactivity in a subset of neurons in the granule cell layer of
the dentate gyrus in brown
Anti-THY1 polyclonal antibody (HPA003733) staining of human cerebral cortex shows
localization to neuropils in brown
Product Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-ENG AMAb90925 Monoclonal IHC 66 22Anti-ENG HPA011862 Polyclonal IHCWB 66 22Anti-FABP7 AMAb90595 Monoclonal IHCWB 89 90Anti-FABP7 HPA028825 Polyclonal IHCWB 89 90Anti-FLT1 AMAb90704 Monoclonal IHCWB 80 82Anti-GAP43 HPA015600 Polyclonal IHC ICC-IF 71 70Anti-GLI3 HPA005534 Polyclonal IHC 74 76Anti-MEF2C AMAb90727 Monoclonal IHCWBICC-IF 97 47Anti-MEF2C HPA005533 Polyclonal IHCWBICC-IF 97 47Anti-MKI67 (Ki67) HPA000451 Polyclonal IHCICC-IF 66 67Anti-NACC1 HPA021238 Polyclonal IHCICC-IF 91 89Anti-NES (Nestin) AMAb90556 Monoclonal IHCWBICC-IF 47 42Anti-NES (Nestin) HPA007007 Polyclonal IHCWB 47 42Anti-NKX2-2 HPA003468 Polyclonal IHCWB 96 97Anti-PAX6 HPA030775 Polyclonal IHCICC-IF 100 100Anti-PBK HPA005753 Polyclonal IHCWBICC-IF 91 94Anti-REST AMAb90740 Monoclonal IHCICC-IF 41 43Anti-RUNX1 HPA004176 Polyclonal IHCICC-IF 93 93Anti-RUNX2 AMAb90591 Monoclonal IHCWBICC-IF 100 81Anti-RUNX2 HPA022040 Polyclonal IHCWBICC-IF 100 81Anti-SATB2 AMAb90679 Monoclonal IHCWBICC-IF 100 100Anti-SATB2 HPA029543 Polyclonal IHCWBICC-IF 100 100Anti-SOX4 HPA029901 Polyclonal IHCICC-IF 100 39Anti-SOX6 HPA001923 Polyclonal IHCICC-IF 96 96Anti-SOX7 HPA009065 Polyclonal IHCWB 91 91Anti-SOX9 AMAb90795 Monoclonal IHCWBICC-IF 97 96Anti-SOX11 AMAb90502 Monoclonal IHCWB 82 82Anti-SOX11 HPA000536 Polyclonal IHCWB 82 82Anti-SOX30 HPA006159 Polyclonal IHCWB 68 70Anti-THY1 AMAb90844 Monoclonal IHCWB 64 68Anti-THY1 HPA003733 Polyclonal IHCICC-IF 64 68Anti-TM4SF2TSPAN7 HPA003140 Polyclonal IHCWB 96 96Anti-TM4SF2TSPAN7 AMAb90621 Monoclonal IHCWB 96 96Anti-VANGL1 AMAb90600 Monoclonal WBICC-IF 95 95Anti-VIM AMAb90516 Monoclonal IHCWB 99 99Anti-VIM HPA001762 Polyclonal IHCWBICC-IF 99 99
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Development
Products with enhanced validation for indicated application (see page 16)
15
At Atlas Antibodies we extensively validate our antibodies in IHC WB and ICC-IF Enhanced Validation is performed as an additional layer of security in an application and context-specific manner Enhanced validation offers increased security of antibody specificity in a defined context This is ensured by using the most relevant validation method for each combination of protein sample and application Enhanced Validation follows the guidelines proposed by the International Working Group for Antibody Validation (IWGAV)
ENHANCED VALIDATION
GENETIC VALIDATION Antibody specificity is confirmed by downregulating the target protein on a genetic level using siRNA
ORTHOGONAL VALIDATIONThe antibody is validated by comparing the results with a non-antibody based method across multiple samples
VALIDATION BY INDEPENDENT ANTIBODIESThe antibody specificity is demonstrated by comparing two antibodies targeting different regions of the same protein
RECOMBINANT EXPRESSION VALIDATIONThe antibody binding is verified using and over-expressed or tagged version of the target protein
MIGRATION CAPTURE MS VALIDATIONThe staining pattern and the protein size detected by the antibody is compared with results obtained by a capture Mass Spectrometry (MS) method
16
Triple A PolyclonalsTM are rabbit polyclonal primary antibodies developed within the Human Protein Atlas project IHC characterization data from 44 normal and 20 cancer tissues is available on the Human Protein Atlas portal
PrecisA MonoclonalsTM are mouse monoclonal primary antibodies developed against a number of carefully selected targets Clones are selected to recognize only unique non-overlapping epitopes and isotypes
Atlas Antibodies ABVoltavaumlgen 13SE-168 69 BrommaSwedenatlasantibodiescom
Phone +46(0)8 54 59 58 50contactatlasantibodiescomorderatlasantibodiescomsupportatlasantibodiescom
Atlas Antibodies logo Triple A Polyclonals PrecisA Monoclonals PrEST Antigen and QPrEST are trademarks or registered trademarks of Atlas Antibodies AB All other trademarks are the property of their respective owners
Products are for research use only Not for use in diagnostic procedures copy Atlas Antibodies AB 2020 008_
Neu
ro M
arke
r Pan
el _
Rev
03_2
0121
5_S
fN E
ditio
n
The development of the central nervous system includes the development of the brain spinal cord optic and auditory systems
Within the neural tube stem cells generate the two major classes of cells that make the majority of the nervous system neurons and glia Both these classes of cells differentiate into many different types generated with highly specialized functions and shapes
The development of the peripheral and central nervous systems begins early in embryogenesis and can be traced through the use of lineage antibodies specific to each stage of neuronal development
The (SOX) family of transcription factors are well-established regulators of cell fate decisions during development
Vimentin (VIM) is a type III intermediate filament protein expressed in mesenchymal cells It is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both healthy development and metastatic progression
The cell surface glycoprotein Thy1 (CD90) is expressed on many mammalian cell types including neurons and serves as a human mesenchymal stem cell (MSC) marker
DEVELOPMENT
A
nti-VIM antibody
A
nti-SOX4 antibody
Anti-T
HY1 antibody
Anti-VIM polyclonal antibody (HPA001762) immunofluorescent staining of human cell line
ASC TERT1 shows localization to intermediate filaments in green
Anti-SOX4 monoclonal antibody (AMAb913802) immunofluorescent staining in mouse
hippocampus showing immunoreactivity in a subset of neurons in the granule cell layer of
the dentate gyrus in brown
Anti-THY1 polyclonal antibody (HPA003733) staining of human cerebral cortex shows
localization to neuropils in brown
Product Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-ENG AMAb90925 Monoclonal IHC 66 22Anti-ENG HPA011862 Polyclonal IHCWB 66 22Anti-FABP7 AMAb90595 Monoclonal IHCWB 89 90Anti-FABP7 HPA028825 Polyclonal IHCWB 89 90Anti-FLT1 AMAb90704 Monoclonal IHCWB 80 82Anti-GAP43 HPA015600 Polyclonal IHC ICC-IF 71 70Anti-GLI3 HPA005534 Polyclonal IHC 74 76Anti-MEF2C AMAb90727 Monoclonal IHCWBICC-IF 97 47Anti-MEF2C HPA005533 Polyclonal IHCWBICC-IF 97 47Anti-MKI67 (Ki67) HPA000451 Polyclonal IHCICC-IF 66 67Anti-NACC1 HPA021238 Polyclonal IHCICC-IF 91 89Anti-NES (Nestin) AMAb90556 Monoclonal IHCWBICC-IF 47 42Anti-NES (Nestin) HPA007007 Polyclonal IHCWB 47 42Anti-NKX2-2 HPA003468 Polyclonal IHCWB 96 97Anti-PAX6 HPA030775 Polyclonal IHCICC-IF 100 100Anti-PBK HPA005753 Polyclonal IHCWBICC-IF 91 94Anti-REST AMAb90740 Monoclonal IHCICC-IF 41 43Anti-RUNX1 HPA004176 Polyclonal IHCICC-IF 93 93Anti-RUNX2 AMAb90591 Monoclonal IHCWBICC-IF 100 81Anti-RUNX2 HPA022040 Polyclonal IHCWBICC-IF 100 81Anti-SATB2 AMAb90679 Monoclonal IHCWBICC-IF 100 100Anti-SATB2 HPA029543 Polyclonal IHCWBICC-IF 100 100Anti-SOX4 HPA029901 Polyclonal IHCICC-IF 100 39Anti-SOX6 HPA001923 Polyclonal IHCICC-IF 96 96Anti-SOX7 HPA009065 Polyclonal IHCWB 91 91Anti-SOX9 AMAb90795 Monoclonal IHCWBICC-IF 97 96Anti-SOX11 AMAb90502 Monoclonal IHCWB 82 82Anti-SOX11 HPA000536 Polyclonal IHCWB 82 82Anti-SOX30 HPA006159 Polyclonal IHCWB 68 70Anti-THY1 AMAb90844 Monoclonal IHCWB 64 68Anti-THY1 HPA003733 Polyclonal IHCICC-IF 64 68Anti-TM4SF2TSPAN7 HPA003140 Polyclonal IHCWB 96 96Anti-TM4SF2TSPAN7 AMAb90621 Monoclonal IHCWB 96 96Anti-VANGL1 AMAb90600 Monoclonal WBICC-IF 95 95Anti-VIM AMAb90516 Monoclonal IHCWB 99 99Anti-VIM HPA001762 Polyclonal IHCWBICC-IF 99 99
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Development
Products with enhanced validation for indicated application (see page 16)
15
At Atlas Antibodies we extensively validate our antibodies in IHC WB and ICC-IF Enhanced Validation is performed as an additional layer of security in an application and context-specific manner Enhanced validation offers increased security of antibody specificity in a defined context This is ensured by using the most relevant validation method for each combination of protein sample and application Enhanced Validation follows the guidelines proposed by the International Working Group for Antibody Validation (IWGAV)
ENHANCED VALIDATION
GENETIC VALIDATION Antibody specificity is confirmed by downregulating the target protein on a genetic level using siRNA
ORTHOGONAL VALIDATIONThe antibody is validated by comparing the results with a non-antibody based method across multiple samples
VALIDATION BY INDEPENDENT ANTIBODIESThe antibody specificity is demonstrated by comparing two antibodies targeting different regions of the same protein
RECOMBINANT EXPRESSION VALIDATIONThe antibody binding is verified using and over-expressed or tagged version of the target protein
MIGRATION CAPTURE MS VALIDATIONThe staining pattern and the protein size detected by the antibody is compared with results obtained by a capture Mass Spectrometry (MS) method
16
Triple A PolyclonalsTM are rabbit polyclonal primary antibodies developed within the Human Protein Atlas project IHC characterization data from 44 normal and 20 cancer tissues is available on the Human Protein Atlas portal
PrecisA MonoclonalsTM are mouse monoclonal primary antibodies developed against a number of carefully selected targets Clones are selected to recognize only unique non-overlapping epitopes and isotypes
Atlas Antibodies ABVoltavaumlgen 13SE-168 69 BrommaSwedenatlasantibodiescom
Phone +46(0)8 54 59 58 50contactatlasantibodiescomorderatlasantibodiescomsupportatlasantibodiescom
Atlas Antibodies logo Triple A Polyclonals PrecisA Monoclonals PrEST Antigen and QPrEST are trademarks or registered trademarks of Atlas Antibodies AB All other trademarks are the property of their respective owners
Products are for research use only Not for use in diagnostic procedures copy Atlas Antibodies AB 2020 008_
Neu
ro M
arke
r Pan
el _
Rev
03_2
0121
5_S
fN E
ditio
n
Product Name
Product Number Clonality Validated
ApplicationsSequence Identity mouse rat
Anti-ENG AMAb90925 Monoclonal IHC 66 22Anti-ENG HPA011862 Polyclonal IHCWB 66 22Anti-FABP7 AMAb90595 Monoclonal IHCWB 89 90Anti-FABP7 HPA028825 Polyclonal IHCWB 89 90Anti-FLT1 AMAb90704 Monoclonal IHCWB 80 82Anti-GAP43 HPA015600 Polyclonal IHC ICC-IF 71 70Anti-GLI3 HPA005534 Polyclonal IHC 74 76Anti-MEF2C AMAb90727 Monoclonal IHCWBICC-IF 97 47Anti-MEF2C HPA005533 Polyclonal IHCWBICC-IF 97 47Anti-MKI67 (Ki67) HPA000451 Polyclonal IHCICC-IF 66 67Anti-NACC1 HPA021238 Polyclonal IHCICC-IF 91 89Anti-NES (Nestin) AMAb90556 Monoclonal IHCWBICC-IF 47 42Anti-NES (Nestin) HPA007007 Polyclonal IHCWB 47 42Anti-NKX2-2 HPA003468 Polyclonal IHCWB 96 97Anti-PAX6 HPA030775 Polyclonal IHCICC-IF 100 100Anti-PBK HPA005753 Polyclonal IHCWBICC-IF 91 94Anti-REST AMAb90740 Monoclonal IHCICC-IF 41 43Anti-RUNX1 HPA004176 Polyclonal IHCICC-IF 93 93Anti-RUNX2 AMAb90591 Monoclonal IHCWBICC-IF 100 81Anti-RUNX2 HPA022040 Polyclonal IHCWBICC-IF 100 81Anti-SATB2 AMAb90679 Monoclonal IHCWBICC-IF 100 100Anti-SATB2 HPA029543 Polyclonal IHCWBICC-IF 100 100Anti-SOX4 HPA029901 Polyclonal IHCICC-IF 100 39Anti-SOX6 HPA001923 Polyclonal IHCICC-IF 96 96Anti-SOX7 HPA009065 Polyclonal IHCWB 91 91Anti-SOX9 AMAb90795 Monoclonal IHCWBICC-IF 97 96Anti-SOX11 AMAb90502 Monoclonal IHCWB 82 82Anti-SOX11 HPA000536 Polyclonal IHCWB 82 82Anti-SOX30 HPA006159 Polyclonal IHCWB 68 70Anti-THY1 AMAb90844 Monoclonal IHCWB 64 68Anti-THY1 HPA003733 Polyclonal IHCICC-IF 64 68Anti-TM4SF2TSPAN7 HPA003140 Polyclonal IHCWB 96 96Anti-TM4SF2TSPAN7 AMAb90621 Monoclonal IHCWB 96 96Anti-VANGL1 AMAb90600 Monoclonal WBICC-IF 95 95Anti-VIM AMAb90516 Monoclonal IHCWB 99 99Anti-VIM HPA001762 Polyclonal IHCWBICC-IF 99 99
Selected PrecisA MonoclonalsTM and Triple A PolyclonalsTM for Development
Products with enhanced validation for indicated application (see page 16)
15
At Atlas Antibodies we extensively validate our antibodies in IHC WB and ICC-IF Enhanced Validation is performed as an additional layer of security in an application and context-specific manner Enhanced validation offers increased security of antibody specificity in a defined context This is ensured by using the most relevant validation method for each combination of protein sample and application Enhanced Validation follows the guidelines proposed by the International Working Group for Antibody Validation (IWGAV)
ENHANCED VALIDATION
GENETIC VALIDATION Antibody specificity is confirmed by downregulating the target protein on a genetic level using siRNA
ORTHOGONAL VALIDATIONThe antibody is validated by comparing the results with a non-antibody based method across multiple samples
VALIDATION BY INDEPENDENT ANTIBODIESThe antibody specificity is demonstrated by comparing two antibodies targeting different regions of the same protein
RECOMBINANT EXPRESSION VALIDATIONThe antibody binding is verified using and over-expressed or tagged version of the target protein
MIGRATION CAPTURE MS VALIDATIONThe staining pattern and the protein size detected by the antibody is compared with results obtained by a capture Mass Spectrometry (MS) method
16
Triple A PolyclonalsTM are rabbit polyclonal primary antibodies developed within the Human Protein Atlas project IHC characterization data from 44 normal and 20 cancer tissues is available on the Human Protein Atlas portal
PrecisA MonoclonalsTM are mouse monoclonal primary antibodies developed against a number of carefully selected targets Clones are selected to recognize only unique non-overlapping epitopes and isotypes
Atlas Antibodies ABVoltavaumlgen 13SE-168 69 BrommaSwedenatlasantibodiescom
Phone +46(0)8 54 59 58 50contactatlasantibodiescomorderatlasantibodiescomsupportatlasantibodiescom
Atlas Antibodies logo Triple A Polyclonals PrecisA Monoclonals PrEST Antigen and QPrEST are trademarks or registered trademarks of Atlas Antibodies AB All other trademarks are the property of their respective owners
Products are for research use only Not for use in diagnostic procedures copy Atlas Antibodies AB 2020 008_
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At Atlas Antibodies we extensively validate our antibodies in IHC WB and ICC-IF Enhanced Validation is performed as an additional layer of security in an application and context-specific manner Enhanced validation offers increased security of antibody specificity in a defined context This is ensured by using the most relevant validation method for each combination of protein sample and application Enhanced Validation follows the guidelines proposed by the International Working Group for Antibody Validation (IWGAV)
ENHANCED VALIDATION
GENETIC VALIDATION Antibody specificity is confirmed by downregulating the target protein on a genetic level using siRNA
ORTHOGONAL VALIDATIONThe antibody is validated by comparing the results with a non-antibody based method across multiple samples
VALIDATION BY INDEPENDENT ANTIBODIESThe antibody specificity is demonstrated by comparing two antibodies targeting different regions of the same protein
RECOMBINANT EXPRESSION VALIDATIONThe antibody binding is verified using and over-expressed or tagged version of the target protein
MIGRATION CAPTURE MS VALIDATIONThe staining pattern and the protein size detected by the antibody is compared with results obtained by a capture Mass Spectrometry (MS) method
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Triple A PolyclonalsTM are rabbit polyclonal primary antibodies developed within the Human Protein Atlas project IHC characterization data from 44 normal and 20 cancer tissues is available on the Human Protein Atlas portal
PrecisA MonoclonalsTM are mouse monoclonal primary antibodies developed against a number of carefully selected targets Clones are selected to recognize only unique non-overlapping epitopes and isotypes
Atlas Antibodies ABVoltavaumlgen 13SE-168 69 BrommaSwedenatlasantibodiescom
Phone +46(0)8 54 59 58 50contactatlasantibodiescomorderatlasantibodiescomsupportatlasantibodiescom
Atlas Antibodies logo Triple A Polyclonals PrecisA Monoclonals PrEST Antigen and QPrEST are trademarks or registered trademarks of Atlas Antibodies AB All other trademarks are the property of their respective owners
Products are for research use only Not for use in diagnostic procedures copy Atlas Antibodies AB 2020 008_
Neu
ro M
arke
r Pan
el _
Rev
03_2
0121
5_S
fN E
ditio
n
Triple A PolyclonalsTM are rabbit polyclonal primary antibodies developed within the Human Protein Atlas project IHC characterization data from 44 normal and 20 cancer tissues is available on the Human Protein Atlas portal
PrecisA MonoclonalsTM are mouse monoclonal primary antibodies developed against a number of carefully selected targets Clones are selected to recognize only unique non-overlapping epitopes and isotypes
Atlas Antibodies ABVoltavaumlgen 13SE-168 69 BrommaSwedenatlasantibodiescom
Phone +46(0)8 54 59 58 50contactatlasantibodiescomorderatlasantibodiescomsupportatlasantibodiescom
Atlas Antibodies logo Triple A Polyclonals PrecisA Monoclonals PrEST Antigen and QPrEST are trademarks or registered trademarks of Atlas Antibodies AB All other trademarks are the property of their respective owners
Products are for research use only Not for use in diagnostic procedures copy Atlas Antibodies AB 2020 008_
Neu
ro M
arke
r Pan
el _
Rev
03_2
0121
5_S
fN E
ditio
n
