Nahla S Barakat, PhD Professor of Pharmaceutics 9/19/2015 PHR 416 1 Principles and kinetics of drug...
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Transcript of Nahla S Barakat, PhD Professor of Pharmaceutics 9/19/2015 PHR 416 1 Principles and kinetics of drug...
Nahla S Barakat, PhDProfessor of Pharmaceutics 04/19/23PHR 4161
Principles and kinetics of
drug stability (PHR 416)
Course Description:The course deals with different routes of drug
degradation principles and kinetics of chemical degradation and stress stability testing.
Means of prolonging shelf life of pharmaceutical products are also included.
04/19/23PHR 4162
Recommended text books:Martin’s Physical Pharmacy & Pharmaceutical
Sciences, Fifth Edition, Patrik J. Sinko (ED), Lippincott Williams & Wilkins 2006,( Chapter 15).
Modern Pharmaceutics, Fourth Edition, G. S. Banker, C. T, Rhods. Marcl Dekker In., 2002
Physicochemical principles of Pharmacy, Fourth Edition, A.T Florence, D. Attwood, Pharmaceutical Press, 2006, (Chapter 4)
Recommended References :US Pharmacopea
<1191> Stability consideration in dispensing practice
04/19/23PHR 4163
This course is a 3 credit hour subject & correspond to 300 marks The marks are divided as follows: 1- Final exam ………………..120 Marks 2- Oral exam ………………… 30 Marks 2- Mid term …………………. 60 Marks 3- Practical…………………... 90 Marks
04/19/23PHR 4164
Introduction
04/19/23PHR 4165
Basic requirements of pharmaceutical products
Efficacy: Optimum therapeutic level for specified period of time.
Safety: Minimum or no side effects. Stability: The products should retain their
properties during storage.
04/19/23PHR 4166
The USP defines the stability of
pharmaceutical product as “extent to
which a product retains within specified
limits” and throughout its period of storage
and use (i.e its shelf life) the same
properties and characteristics that it
possessed at the time of its manufacturer
Stability of drug also can be defined as the time from the date of manufacture and packaging of the formulation until its chemical or predetermined level of labelled potency and its physical characteristics have not changed appreciably.
For a drug substance, we need to study 3 categories of stabilities-
A. Solid state stability of drug only B. Compatibility studies ( drug+ excipients ) C. Solution phase stability
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04/19/23PHR 4168
These stability data involves selected parameters that taken together from the stability profile.
Pharmaceutical products are expected to meet their specification for identifying purity, quality and strength throughout their defined storage period at specific storage condition.
The stability of pharmaceutical product is investigated throughout the various stages of the development process.
Importance of stability studies
04/19/23PHR 4169
Development of optimum formulation(preformulation studies) Finding the optimum storage conditions(temperature, light, humidity). Selecting the proper container for dispensing(glass or plastic, clear or opaque, cap liners). Predicting the shelf life of the drug. Anticipating drug excipient interactions. Stabilization of the drugs against degradation
04/19/23PHR 41610
In some cases a pharmacist may need to
prepare stable compounded preparations
from existing dosage form.
It is the responsibility of the pharmacist
via the information of the manufacture to
instruct the patient in the proper storage
and handling of the drug product.
Factors affecting drug stability:1. Temperature: high temperature accelerate oxidation, reduction
and hydrolysis reaction which lead to drug degradation2. pH:• Acidic and alkaline pH influence the rate of decomposition of
most drugs.• Many drugs are stable between pH 4 and 8.• Weekly acidic and basic drugs show good solubility when they
are ionized and they also decompose faster when they are ionized.
• Sometimes pH can have a very serious effect on decomposition. As little as 1 pH unit change in pH can cause a change of ten fold in rate constant. So when we are formulating a drug into a solution we should carefully prepare a pH – decomposition profile
04/19/23PHR 41611
3. Moisture:a. Water catalyses chemical reactions as
oxidation, hydrolysis and reduction reactionb. Water promotes microbial growth4. Light: affects drug stability through its energy
or thermal effect which lead to oxidation5. Pharmaceutical dosage forms: solid dosage
forms are more stable than liquid dosage forms for presence of water.
6. Concentration: rate of drug degradation is constant for the solutions of the same drug with different concentration. So, ratio of degraded part to total amount of drug in diluted solution is bigger than of concentrated solution.
04/19/23PHR 41612
7. Drug incompatibility: reactions between components of pharmaceutical dosage forms it self or between these components and cover of the container .
8. Oxygen: exposure of drug formulations to oxygen affects their stability
04/19/23PHR 41613
Expiry date: means that drug can not be used after this date because the concentration of drug is decreased and become lower than therapeutic concentration. In addition, some products of drug degradation are toxic and harmful to patients.
NOTE: The expiration date period should begin at the time of manufacture of the lot.
PRODUCT TYPE MAX. TIME PERIODDosage forms: 5 years Implants, injectables, tablets, capsules, soluble
powders, etc. • Note! After the opening of the drug container, the
expiry date will be shorter as a result of the decreased concentration of drug during usage and the effects of external factors. :
04/19/2314 PHR 416
Examples: 1. Eye drops: can be used for one month after
opening the droppers2. Syrups and suspension of antibiotics: can be
used for one week by storage in room temperature and for two weeks by storage in 4C°.
3. Tablets and capsules remain stable in the package but after removal the expiry date will change
4. Ampoules: must be used immediately but the vials (multidose) are stable for 24 h for the presence of preservatives.
04/19/23PHR 41615
Five stabilities of drug must be considered::
1. Physical2. Chemical3. Microbiological4. Toxicological5. Therapeutic
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Reaction kinetics:
04/19/23PHR 41618
“kinetic” originates fromGreek “kinetikos” that, in turn, originates from Greek “kinetos’ which means “moving”.
Kinetics: It is the study of how a system changes as function of time.
Reaction kinetics: It is the study of rate of chemical change and the way in which this rate is influenced by conditions of
concentration of reactants and products, solvent, ionic strength and temperature
Rate and order of reactions
04/19/23PHR 41619
Importance of the rate process: For drug manufacturer as he must
demonstrate that his product is stable and can be stored for reasonable length of time without changing to inactive or toxic form.
The pharmacist must be aware of potential instability of the drug that he handles.
The physician and the patient must be assured that the prescribed drug will reach the site of action in sufficient concentration.
Rate and order of reactions
04/19/23PHR 41620
Fields of rate process:Stability and incompatibility: Here the rate
process can lead to inactivation of the drug through decomposition or conversion into inactive or toxic form.
Dissolution: Here the main concern is the rapidity with which a solid dosage form is changed to molecular solution.
04/19/23PHR 41621
Pharmacokinetics: Concerns with the rate of drug absorption, elimination and metabolism.
Drug action at molecular level: Here it is assumed that generation of a response by a drug is a rate process.
In general, reaction kinetics is the study of rate of chemical change and the way in which this rate is influenced by conditions of concentration of reactants, products and other chemical species which may be present, and the factors such as solvent, pressure and temperature.
Reaction kinetics permits formulation of models for the intermediate steps through which reactants are converted into other chemical compounds and;
is a powerful tool in elucidating the mechanism by which chemical reactions proceed.
04/19/23PHR 41622
It provides a rational approach to stabilization of drug products and prediction of shelf- life and optimum storage conditions. e.g. thiamine HCl is most stable at pH 2-3 and is unstable at pH above 6. If this is combined with a buffered vehicle of say pH 8 or 9 the vitamin is rapidly inactivated.
Knowing the rate at which a drug deteriorates at various hydrogen ion concentrations allows one to choose a vehicle that will retard or prevent the degradation.
04/19/23PHR 41623
Reaction Rate The rate of reaction is the velocity with
which a reactant or reactants undergo chemical change.
The rate, velocity or speed of a reaction is given by the expression dc / dt.
where dc is increase or decrease of concentration over a time interval dt
04/19/23PHR 41624
Factors that affect the reaction rate: 1- concentration of the reactant 2- solubility of drug3- pressure (gases)4- surface area5- presence of catalyst6- Nature of the reactant7- Temperature 8- pH 9- light and humidity
04/19/23PHR 41625
Rate and order of reaction
04/19/23PHR 41626
Rate: The rate, velocity or speed of reaction is
given by:
dc/dt This expression gives the increase (+) or
decrease (-) in concentration (C ) within a given time intervals (dt )
04/19/23PHR 41627
Reaction kinetics: Rate According to the law of mass action: The rate of a chemical reaction is proportional
to the product of molar concentrations of the reactants each raised to a power equal to the number of molecules of the substance undergoing reaction.
aA + bB + ….. = Products Rate = k [A]a [B]b
Where k is rate constant.
Order of reaction
04/19/23PHR 41628
Reactions are classified according to number of reacting species whose concentration determines the rate at which the reaction occurs, i.e. the order of reaction.
aA + bB ProductReaction rate = K [A]a [B]b
* If a=2 and b=1, the reaction rate = K [A]2[B]1
* The reaction is second order with respect to A and first order with respect to B.
* The overall order is the sum of the exponents of concentration terms that afford a linear plot, i.e. third order
04/19/23PHR 41629
CH3COOC2H5 + NaOHsoln → CH3COONa + C2H5OH
• The reaction is first order (a = 1) with respect to ethyl acetate and first order (b= 1) with respect to sodium hydroxide solution.
• The overall reaction is second order (a + b = 2)
04/19/23PHR 41630
CH3COOC2H5 + xss NaOHsoln → CH3COONa + C2H5OH
• Suppose that NaOH solution is used as solvent (i.e. its conc. is very high) and ethyl acetate were in low concentration. As the reaction proceeds, ethyl acetate would change appreciably from its original concentration,
Whereas the concentrations of NaOH solution would remain
essentially unchanged because of its presence in great excess.
The reaction is then said to be pseudo-first-orderreaction because it depends only on the first power (a
= 1) of the concentration of ethyl acetate
Zero order reaction:The zero order rate law for the general reaction A P In this type of reaction the decomposition
processed at a constant rate and its independent of the concentrations of any of the reactants, the rate equation is :
- d [A]= k0 (1)
dt Which on integration of both sides gives:
When t= 0 the concentration of A is [A]0
[A]t = A0 – k0 t (2)
04/19/23PHR 41631
K0 = A0 – At
t A plot of the amount remaining [A] against time
is linear with a slope of -k0 (concentration. Time -1)
Many decomposition reactions in the solidPhase or in suspensions apparentlyfollow zero-order kinetics. Unit of k0= moles/liter
secondmoles. liter-1 .second-1
04/19/23PHR 41632
First order reaction
A general unimolecular reaction A Pwhere A is a reactant and P is a product is called a first-
order reaction. The rate is proportional to the concentration of a single reactant raised to the first power.
The decrease in the concentration of A over time can be written as:
V = d [A] = k1 [A] (1)
d t
04/19/23PHR 41633
- d[A] = k dt (2) [ A]
Integrating the equation (2) yield :
Ln A- ln A0 = -k (t- 0)
Ln A = ln A0 –kt Ln [A] = - k t [A]0
04/19/23PHR 41634
Plot ln [A] or ln [A] / [A] 0 against time (t) create a straight line with slope – k
Log A = log A0 – kt /2.303
K = 2.303 log A0
t ASlope = -k/2.303k1 has the dimensions of time -1.
Equation can be written as[A] = [A]0 e –kt
This means that the concentration of A decrease exponentially as afunction of time.
04/19/23PHR 41635
Pseudo First Order Reaction Even in the case of a reaction involving more than one reacting
species, the rate may still follow first-order kinetics. The most common example of this occurs when one of the reactants is in such a large excess that any change in its concentration is negligible compared with changes in the concentration of the other reactants.
This type of reaction is termed a pseudo first-order reaction. Such reactions are often met in stability studies of drugs that hydrolyze in solution, the water being in such excess that changes in its concentration are negligible and hence the rate of reaction is dependent solely on the drug concentration.
04/19/23PHR 41636
Assignment 2 EXAMPLE.1 Calculation of first-order rate
constantand half-lifeThe following data were obtained for the hydrolysis of
homatropine in 0.226 mol dm-3 HCl at 90°C: Percentage
homatropin remaining 93.4 85.2 75.9 63.1 52.5 41.8
Time (h) 1.38 3.0 6.0 8.6 12 17
04/19/23PHR 41637
04/19/23PHR 41638
04/19/23PHR 41639
Second order reactionThe rate of a second-order reaction is
determinedby the concentrations of two reacting species. For reactions in which both concentration
terms refer to the same reactant we may write:- d [A] = k 2 [A] 2 (1)
- dt- and dx = k2 (a-x)2 (2)
- dt
04/19/23PHR 41640
Integration of equation 2 between limits of t from 0 to t and of x from 0 to x yield:
t = I 1 - 1 = x k2 a-x a k2 a (a-x)
k2 t = 1 - 1
A A0
A plot of t against x/a(a-x) yields a linear plot of slope 1/k2
K2 has unit concentration -1 time -1
04/19/23PHR 41641
Determination of the order of reactiondata may be displayed graphically according to
the linearequations for the various orders of reactions
until a straight-line plot is obtained. Thus, for example, if the data yield a linear graph when plotted as t against log(a- x) the reaction is
then taken to be first-order.An alternative method of determining the order
ofreaction, is based on equation :
04/19/23PHR 41642
The half-life of the reaction is determined for a series of initial drug concentrations, C0 , and the order, n, is calculated from the slope ofplots of log t0.5 as a function of log C0 .
Assignment 2 Example: The kinetics of decomposition of a drug in aqueous
solution were studied using a series of solutions of different initial drug concentrations,
C0 . For each solution the time taken for half the drug to decompose (that is, t0.5) was determined with the following results:
C0 (mol dm -3) 4.625 1.698 0.724 0.288
t0.5 (min) 87.17 240.1 563.0 1414.4
Determine the order of reaction and calculate the rate constant
04/19/23PHR 41643