Mycobacteria (molecular) Laboratory : Distribution : 3892 ...London NW9 1GH Published at 11:28 on...
Transcript of Mycobacteria (molecular) Laboratory : Distribution : 3892 ...London NW9 1GH Published at 11:28 on...
Dispatch Date : 11-Apr-2016
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Mycobacteria (molecular)
Distribution : 3892
Laboratory :
Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG
© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 11:28 on Monday 20 June 2016
Your Score Intended Result Your Report
Specimen 3151 Direct detection overall report
Mycobacteria not detected Mycobacteria not detected 2
Detection post culture overall report
Mycobacteria not detected Mycobacteria not detected 2
Specimen 3152 Direct detection overall report
Mycobacterium tuberculosis Mycobacterium tuberculosis 2
Detection post culture overall report
Mycobacterium tuberculosis Mycobacterium tuberculosis 2
Rifampicin resistance Not resistant Not resistant 2
Cumulative score information Total number of specimens sent to you for UK NEQAS for Mycobacteria (molecular) over the last 3 distributions is 6 For these distributions specimen numbers 2770 2771 2894 2895 3151 3152 have been sent. Number of specimens reported too late for analysis (not scored) 0 Your cumulative score for the specimen/test combinations that you reported was 28 out of a possible total of 28 The mean score calculated from the reports returned by UK laboratories testing the specimen/test combinations you examined was 27.06 (with a standard error of 1.80). Performance rating Your performance rating for UK NEQAS for Mycobacteria (molecular) (i.e. the number of standard errors by which your cumulative score lies above or below the mean for UK laboratories) is 0.52. A performance rating of more than 1.96 standard errors below the mean indicates possible poor performance. Your performance rating may change if other participants' results are amended. No score penalty is incurred for non return of reports. However non reporting of results may be used as a measure of poor performance.
Comments: A total of 171 sets of specimens were distributed for testing with 161 participants returning results within the specified period. Specimen 3151: Mycobacteria not detected Detection: Both the direct and post culture detection results for this specimen were very good with 92.1% (139/151) and 98.4% (62/63) of participants correctly reporting mycobacteria not detected, MTBC negative or no growth. Rifampicin resistance testing was not applicable for this specimen. Specimen 3152: MTBC detected (M. tuberculosis) Detection: Both the direct and post culture detection results for this specimen were excellent with 97.9% (143/146) and 100% (68/68), of participants correctly reporting MTBC or M. tuberculosis detected. Typing results: Typing results received for specimen 3152 correlated well with one another. Tables summarising the results are provided on the last page of this report. The octal designations reported for specimen 3152 correlated well with one another. The following profiles were reported 577777777760771 (n=1), 777777777760771 (n=1) and 777771777760771 (n=1). Turn around time: The time taken to report your results was 0 days. This information is provided for your own use and does not form part of your performance assessment. Enquiries: Pre-distribution test results are available should you experience a technical failure and wish to discuss the results. Written enquiries about this distribution should be addressed to Christine Walton at the email address below. For repeat specimens please order using the web form or e-mail [email protected] stating your laboratory identification number, the distribution name, distribution number and specimen numbers. Acknowledgements: We thank colleagues from Public Health Wales Mycobacterium Reference Unit, Cardiff for their kind assistance with source material and pre-distribution testing. Report authorised by: Christine Walton, Scheme Organiser
Per
form
ance
Rat
ing
Distribution
Total score you achieved for each of the last 12 distributions (4 year cycle)Your current performance rating is 0.35
MICPERF
3102 3145 3223 3292 3339 3458 3520 3561 3669 3732 3775 3892-4-3-2-101234
All
UK
Tot
al s
core
Distribution
Total score you achieved for each of the last 12 distributionsYour total score for this distribution is 10
MICPERF2X
3102 3145 3223 3292 3339 3458 3520 3561 3669 3732 3775 3892-3
0
3
6
9
12
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Dispatch Date : 11-Apr-2016
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Mycobacteria (molecular)
Distribution : 3892
Laboratory :
Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG
© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 11:28 on Monday 20 June 2016
Two specimens of freeze-dried simulated purulent sputa were dispatched with a request for the molecular detection and typing of mycobacteria. Specimen 3151 was negative for mycobacteria by culture and molecular detection and contained Streptococcus mitis and Streptococcus oralis commensals. Specimen 3152 contained viable Mycobacterium tuberculosis and Streptococcus mitis and Streptococcus salivarius commensals.
Specimen : 3151 Direct detection: Mycobacteria not detected
UK (%) All (%)
1 (2.0) 1 (0.5)Gen-Probe : MTD1 (2.0) 2 (1.0)Hain: FluoroType0 (0.0) 2 (1.0)Hain: Mycobact CM/AS1 (2.0) 2 (1.0)Sequencing1 (2.0) 4 (2.0)Nanogen AD: Real-Time Alert1 (2.0) 4 (2.0)Real-Time Single target0 (0.0) 4 (2.0)BD : ProbeTec1 (2.0) 6 (3.1)Seegene: Anyplex2 (3.9) 7 (3.6)Hain: MTBDRplus0 (0.0) 12 (6.1)Roche : Cobas TaqMan2 (3.9) 16 (8.2)Unspecified
39 (76.5) 117 (59.7)Cepheid: GeneXpert
Number of reports0 30 60 90 120 150
MTBC detected MOTT detected Not detected Mycobacterium species MTBC negative M. tuberculosis
Your Report : Mycobacteria not detected
Not detected
1
MTBC detected
151
119
Overall Results
02
------------------------------------------------------------
4120
46
------------------------------------------------------------UK
114
0
2MTBC negative
1
All
Total
-1
Score
Your Score : 2
M. tuberculosis
Specimen : 3151 Detection post culture: Mycobacteria not detected
UK (%) All (%)
2 (15.4) 11 (12.5)Gen-Probe: Accuprobe0 (0.0) 1 (1.1)Real-Time Single target0 (0.0) 1 (1.1)PCR: Single target0 (0.0) 1 (1.1)Innogenetics:Innolipa0 (0.0) 1 (1.1)Fujirebio : INNO-LiPA0 (0.0) 2 (2.3)Seegene: Anyplex0 (0.0) 2 (2.3)BD : ProbeTec3 (23.1) 7 (8.0)Hain: MTBC3 (23.1) 9 (10.2)Hain: MTBDRplus0 (0.0) 11 (12.5)Cepheid: GeneXpert0 (0.0) 18 (20.5)Unspecified5 (38.5) 21 (23.9)Hain: Mycobact CM/AS
Number of reports0 6 12 18 24 30
MTBC detected MOTT detected Not detected Mycobacterium species MTBC negative No growth M. tuberculosis
Your Report : Mycobacteria not detected
Not detected
32
MTBC detected
63
8
Overall Results
22
------------------------------------------------------------
122
12
------------------------------------------------------------UK
13
8
2MTBC negative
0
All
Total
No growth
-1
Score
Your Score : 2
Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG
© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 11:28 on Monday 20 June 2016
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Dispatch Date : 11-Apr-2016
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Mycobacteria (molecular)
Distribution : 3892
Laboratory :
Two specimens of freeze-dried simulated purulent sputa were dispatched with a request for the molecular detection and typing of mycobacteria. Specimen 3151 was negative for mycobacteria by culture and molecular detection and contained Streptococcus mitis and Streptococcus oralis commensals. Specimen 3152 contained viable Mycobacterium tuberculosis and Streptococcus mitis and Streptococcus salivarius commensals.
Specimen : 3152 Direct detection: Mtb complex detected
UK (%) All (%)
1 (2.0) 1 (0.5)Gen-Probe : MTD0 (0.0) 2 (1.1)Qiagen: Artus1 (2.0) 2 (1.1)Hain: FluoroType0 (0.0) 2 (1.1)Hain: Mycobact CM/AS1 (2.0) 4 (2.1)Nanogen AD: Real-Time Alert1 (2.0) 4 (2.1)Real-Time Single target0 (0.0) 4 (2.1)BD : ProbeTec1 (2.0) 6 (3.2)Seegene: Anyplex2 (4.0) 7 (3.7)Hain: MTBDRplus0 (0.0) 12 (6.3)Roche : Cobas TaqMan2 (4.0) 15 (7.9)Unspecified
39 (78.0) 115 (60.5)Cepheid: GeneXpert
Number of reports0 30 60 90 120 150
MTBC detected MOTT detected Not detected Mycobacterium species MTBC negative M. tuberculosis
Your Report : Mycobacterium tuberculosis
MTBC negative
46
MTBC detected
17
Overall Results
149
273
------------------------------------------------------------UK
1292
Total
037
All
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2
Score
Your Score : 2
M. tuberculosis
Specimen : 3152 Detection post culture: Mtb complex detected
UK (%) All (%)
2 (12.5) 11 (11.8)Gen-Probe: Accuprobe0 (0.0) 1 (1.1)PCR: Single target0 (0.0) 1 (1.1)Innogenetics:Innolipa0 (0.0) 1 (1.1)Fujirebio : INNO-LiPA0 (0.0) 2 (2.2)Seegene: Anyplex0 (0.0) 2 (2.2)Real-Time Single target0 (0.0) 2 (2.2)BD : ProbeTec4 (25.0) 10 (10.8)Hain: MTBDRplus3 (18.8) 11 (11.8)Hain: MTBC2 (12.5) 15 (16.1)Cepheid: GeneXpert1 (6.3) 15 (16.1)Unspecified4 (25.0) 19 (20.4)Hain: Mycobact CM/AS
Number of reports0 4 8 12 16 20
MTBC detected MOTT detected Not detected Mycobacterium species MTBC negative No growth M. tuberculosis
Your Report : Mycobacterium tuberculosis
MTBC detected
14
Overall Results
68Total
25
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435 2
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9
All
2
Score
M. tuberculosis
Your Score : 2
Specimen : 3152 Rifampicin resistance: Not resistant
UK (%) All (%)
9 (21.4) 25 (21.0)Hain: MTBDRplus0 (0.0) 1 (0.8)Sequencing1 (2.4) 1 (0.8)PCR: Single target0 (0.0) 1 (0.8)Becton Dickinson0 (0.0) 1 (0.8)Beckman: GenomeLab2 (4.8) 6 (5.0)Unspecified
30 (71.4) 84 (70.6)Cepheid: GeneXpert
Number of reports0 20 40 60 80 100
Not relevant Resistant Not resistant
Your Report : Not resistant
Resistant
44
Not relevant
125
Overall Results
130
2441
------------------------------------------------------------UK
40
Total
0Not resistant
0
All
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Score
Your Score : 2
Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG
© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 11:28 on Monday 20 June 2016
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FRM.065203
MOLECULAR DETECTION OF MYCOBACTERIA DISTRIBUTION 3892 Table representation of the typing results received for specimen 3152 Summary of typing results
VNTR Type 0154 0424 0577 0580 0802 0960 1644 1955 2059 2163b 2165 2347 2401
MIRU Type 2 - - 4 40 10 16 - 20 - - - -
ETR profile - - C D - - - - - - A - -
2 2 4 3 3 2 2 3 2 4 2 4 2
2 2 4 2 3 2 2 3 2 4 2 4 2
2 2 4 3 3 2 2 3 2 4 2 4 2
- 1 5 6 0 6 1 6 2 7 6 3 6
continued…
VNTR Type 2461 2531 2687 2996 3007 3171 3192 3690 4052 4156 4348 Number of
participants MIRU Type - 23 24 26 27 - 31 - - - 39
ETR profile B - - - - - E - - - -
1 5 1 4 4 3 3 4 7 2 2 4
1 5 1 4 4 3 3 4 7 2 2 2
1 5 1 4 4 3 3 4 - 2 2 1
2 8 2 5 3 6 4 7 3 1 2 1
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Mycobacteria (molecular)
Distribution : 3892
Laboratory :
Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG
© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 11:28 on Monday 20 June 2016
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