Molecular methods in studying biosystematics in wild...

Molecular methods in studying biosystematics in wild potatoes (Solanum section Petota)

Mirjam Jacobs

Value of wild potatoes

►Potato 4th food crop in the world

►Large secondary genepool (227 – 188 wild species)

► Important source for novel resistance and quality traits | Nematode resistance

| Phytophthora resistance

| Non-enzymatic tuber browning

| Frost tolerance etc.

Geography and Ecology of wild potatoes

► From the southern states of the USA and Mexico to the South of Argentina

► Wide diversity of habitats

| Mountains (800 – 4000 m)

| In deserts

| In cloud forests

| Epiphytic

Classification of Hawkes (1990)

►19 series of tuber-bearing Solanum species

►ordered from primitive to advanced

►Based on morphology , cytology, and crossability

Previous molecular studies

► Molecular studies: RAPD, RFLP, AFLP and DNA sequences

► Most studies only cover a small number of the series described by Hawkes, difficult to link all species together

► cpDNA RFLP studies by Spooner and coworkers: 4 clades

| 1) Mexican diploids,

| 2) S. bulbocastanum and S. cardiophyllum,

| 3) Piurana plus a few other species,

| 4) remaining South American species and polyploid Mexican/North American species

► Elucidate the systematic relationships of wild tuber-bearing Solanum species

| include as many species as possible

| focus on the wild tuber-bearing Solanum accessions in the CGN collection, supplemented with material from other genebanks

| Use the information on the systematic relationships to select material in the second part of the project (search for new sources of R genes against P. infestans)

| Make the information available to others scientists and users (breeders) in CBSG project

► Search for new sources of R genes

| Generate markers for P. infestance resistance

Goal of the project

Original plan:

►Construct ‘backbone’ phylogeny using cpDNA sequencing

►Detailed phylogeny using AFLP

| Scoring in groups based on cpDNA data

But:

►How much variation can be detected in cpDNA ?

►Do AFLP and cpDNA give similar results ?

| Tested on a subset of the samples.

Comparing cpDNA and AFLP: results

cpDNA AFLP

►The cpDNA strict consensus tree is less resolved than the AFLP strict consensus tree

►Some groups appear in both trees

► incongruencies between the AFLP and cpDNA trees

| cpDNA cannot be used for backbone phylogeny in section Petota

| differences probably caused by differences in mode of inheritance: maternal versus biparental, hybridisation

Comparing cpDNA and AFLP: conclusions

Analysis of large AFLP dataset: outline

►1000 accessions, if possible 5 accessions per species, 5 genotypes per accession

| 4929 OTU’s

►Capacity problems

| only UPGMA and NJ could be succesfully applied so far

►New approach: light dataset

| 1 genotype per accession, 916 OTU’s

| mp and NJ jackknife trees (statistical support for found structure)

NJ tree

Complete dataset

global markers AFLP

HAW01462HAW01463 HAW01466HAW01458HAW01464HAW01465 HAW01457 HAW01460HAW01453HAW01455HAW01452 HAW01448HAW01451HAW02478 HAW02480HAW02477 HAW02483HAW02485HAW02486HAW02484 HAW02482HAW01456HAW02481 HAW01449HAW01461HAW01467 HAW01450 HAW01454HAW01469HAW01468HAW01470 HAW01459HAW01471HAW02479INM03644 INM03646INM03642INM03643 INM03645INM04437 INM04438GRL04465 GRL04433 GRL04434GRL04431GRL04417 GRL04418GRL04416GRL04508 GRL04509GRL04493 GRL04494GRL01359GRL04415GRL04466 GRL04463GRL04464 GRL04473 GRL04474GRL04423 GRL04424GRL04421GRL04422 GRL04471 GRL04472GRL04470 VRN04307GRL04469 SPG30160GRL04447GRL30130SCR00714 IFD01563IFD01569 IFD01567 IFD01570IFD01571IFD01568IFD01555IFD01556IFD01553IFD01552IFD01554 IFD01558IFD01559 IFD01560IFD01561IFD01557IFD01562 IFD01566IFD01565 IFD01564OPL01753OPL01756OPL01755 OPL01757OPL01754 IFD04419IFD04420 IFD04451IFD04452 IFD10257IFD10258IFD10259 IFD10256V ID10238V ID10239 GRL04512GRL04513 V ID10241 OPL01748OPL01752OPL01750 OPL01751OPL01749OPL04528 OPL04529OPL04502OPL04503GRL04429 GRL04430OPL04413 OPL04414 OPL04409OPL04453OPL04454OPL04410 OPL04412 OPL01760OPL01762OPL01761OPL01759 OPL01758 OPL01764OPL01766OPL01763OPL01765OPL01767OPL04467OPL04468 GRL04427GRL04428 GRL04475GRL04476GRL04425 GRL04426 GRL04518GRL04519GRL10227 GRL10230GRL10229GRL10228 GRL04449 GRL04450OPL04407GRL04448 GRL04477GRL04478GRL04432GRL04435 GRL04436 V ID00294V ID00295V ID00293V ID10244V ID10242 V ID10243V ID10245V ID01408V ID01409 V ID01410V ID01411V ID01412 GRL04491GRL04489 V ID01405V ID01406 V ID01404 V ID01403V ID01407 V ID01401V ID01402GRL04490 GRL04492 GRL01346GRL01349 GRL01347 GRL01348GRL01361GRL01362GRL01360 GRL01353GRL01354 GRL01356 GRL01355GRL01358GRL01357 GRL01351GRL01352 GRL01339GRL01350GRL01336 GRL01337GRL01338 GRL01335GRL01340 GRL01344GRL01342GRL01341 GRL01345GRL01343 DDS01304DDS01305 DDS01303DDS01302DDS01301PTR01368PTR01372 PTR01370PTR01371 HPS01512 HPS01516HPS01513HPS01514 HPS01515HPS01517 HPS01520HPS01519HPS01521HPS01518HPS01508 HPS01509HPS01507HPS01510HPS01511 INM01527INM01531 INM01528INM01530 INM01522 INM01525INM01529INM01546 INM01526INM01550INM01551 INM01549 INM01523INM01524INM01541INM01533 INM01534INM01532 INM01548INM01537INM01540 INM01538 INM01539INM01536INM01535INM01547 INM01542INM01544INM01545INM01543 V ID01383V ID01386 V ID01384V ID01387 V ID01385 V ID01391V ID01392V ID01389V ID01388 V ID01390V ID01378V ID01379 V ID01382V ID01380V ID01381V ID01393 V ID01394V ID01395 PTR01376PTR01377PTR01373PTR01374PTR01375GRL04516GRL04517 NRS10339NRS10340NRS10343NRS10341NRS10342 NRS10347NRS10344NRS10346 NRS10348NRS10345OPL10359 OPL10360OPL10361 OPL10362OPL10365OPL10366OPL10363OPL10367 NRS03015NRS03016NRS03014 NRS03013 NRS03017VRN04005VRN04009VRN04007VRN04008VRN04006 NRS01725NRS01728NRS01726NRS01724NRS01727PTR03685 GRL04479 AST03151AST03152AST01012 AST01009 AST03154AST01010 AST03155AST03153AST01013AST01011 AST01014 AST01017AST01016 AST03275AST03278 AST01015BLV01107BLV01108 BLV01106BLV01110BLV01109 AST01019AST01020AST01021AST01022AST01023 BLV01101BLV01105BLV01102BLV01103 BLV01104BLV01093 BLV01094 BLV01095BLV01092 AST01007AST03273 AST01008AST01006AST03272AST01004 AST01005AST03270 AST02252AST02253 AST02250AST02251BER01043 AST03271AST01018 AST03277 AST03276AST03274 UGT04275UGT04276UGT04273UGT04274 UGT00732 UGT00731 UGT00735UGT00733 UGT00734HDM00324HDM00325HDM00322 HDM00321HDM00323 HDM03636HDM03635 HDM03637HDM03633HDM03634 HDM03629 HDM03632HDM03628HDM03631 HDM03630HDM03340 HDM03343HDM03341HDM02490 HDM02488HDM02489HDM02491HDM03342 HDM02487HDM03339 HDM01503HDM01504HDM01505 HDM01502HDM01506HDM01501HDM01492HDM01493HDM01494HDM01495 OPL04522 OPL04523GRL04405PTRxLPH03701PTRxLPH03702 PTRxLPH03703PTRxLPH03705PTRxLPH03704 GRL04506GRL04507 PTR03692 PTR03693PTR03691 PTR03694PTR03695 PTR03683PTR03678 PTR03679PTR03676PTR03680 PTR03677PTR10232PTR10236 PTR10235 PTR10233PTR03682PTR10234 PTR03681PTR03684 PTRxLPH10626 PTRxLPH10627PTRxLPH10628 PTRxLPH10625PTRxLPH10624 HPS02493HPS02494HPS02492 HPS02501HPS02495 UGT02862UGT02864UGT02865UGT02861UGT02863 UGT02868 UGT02869UGT02867UGT02870UGT02866 UGT10555UGT10556UGT10558UGT10554 UGT10557UGT20406 UGT20409UGT20407UGT20408 AVL01025 AVL01030AVL01032 AVL03279 AVL03280AVL03282 AVL01024AVL01029AVL01031 AVL03281AVL01027 AVL01028AVL01026AVL01033AVL03283 AVL01036AVL01037AVL01034AVL01035AVL01038 BRCxSPL03589BRCxSPL03601 BRCxSPL03590BRCxSPL03592BRCxSPL03593 OKA01730BRCxSPL03591 BRCxSPL03603BRCxSPL03595V ID10237 V ID10240 SPL01878SPL01879 SPL01880SPL01882SPL01881BERxSPL01041 BRC04504SPL03949 SPL03950 SPL03951SPL03948SPL03947SPL10469 VRG02155VRG02157 VRG02156VRG02159VRG02158VRG02150VRG02154 VRG02152 VRG02153VRG10590VRG02151 VRG10589VRG10592 SPL01884 SPL01886SPL01887SPL01883 SPL01885SPL03960SPL03961SPL03958SPL03959 RAP00545RAP00546RAP00547 RAP00543RAP00544araccpapa02228araccpapa02229 araccpapa02225araccpapa02226 araccpapa02227VRG10593 SPL01888 SPL01890SPLxLPH01920 SPLxLPH01919SPLxLPH01921 SPL01889SPL01891SPL01892 SPL10470SPL10471 SPL10472SPL10473 BRC04445BRC04446SPL04192 SPL04195SPL04201 SPL00684SPL00688 SPL04194SPL00686SPL00687 SPL04193SPL00685 SPL00565 SPL00568SPL00563 SPL00566SPL00570 SPL00564SPL00569SPL00567 SPL00555 SPL00556SPL01894SPL01902SPL00557SPL00562 SPL00558SPL00561SPL00560SPL00559 SPL01893SPL01901SPL01895 LPH01615LPH04091LPH01613 LPH01612LPH04089LPH01616LPH04092 LPH01614LPH04090GRL04406ADG02060 LPHxSPL01621LPHxSPL01623SPLxLPH01918 TUQ20401TUQ20404TUQ20402TUQ20405LPH04095 RCHxMCD10636SPL03957BRCxSPL03602 LPH00826 LPH00827LPH00825 LPH04093LPH04094LPH00828 LPH00363LPH00364 LPH00365LPH00359 LPH00360LPH00361 LPH00362SPL03962SPL03963SPL03964SPL03966SPL03965spp01922 LPH00355LPH00356 LPH00358LPH00357 spp01923spp01924 SCR04247 SCR04249SCR04250 SCR04248SCR04251 SCR30158SCR00722SCR00724 SCR00721SCR00725 SCR00723HDM00329HDM00331 HDM00328HDM00330HDM00326HDM00327HDM00332LPH01607LPH01609LPH01608 LPH01610 LPHxSPL01622 LPH01611LPHxSPL01624ALN03129ALN03130ALN03128 ALN03131 ALN03127SCR01945SCR01948 SCR01946 SCR01949ADG02061 ADG02062ADG02063ADG02064 GON10493GON10505 GON10492GON10489 GON10490 GON10491BRC04457 BRC04458 SCR01941SCR01943SCR01942SCR01940SCR01944ADGxSCR10631 ADGxSCR10632ADGxSCR10629 ADGxSCR10630ADGxSCR10633 PTR03688PTR03690 PTR03689PTR03687 PTR03686SCR04233SCR04236SCR04234 SCR00720SCR04232SCR04235SCR00719 SCR00617SCR00621 SCR00619 SCR00618SCR00616 GRR02473GRR02475GRR02474 GRR02476GRR02472PLT04191SPL03726 SPL03729SPL03728SPL03730SPL03727 HMP02507HMP02508 HMP02509HMP02510 RCH02675RCH02677SCRxOPL03712 SCRxOPL03715SCRxOPL03714SCRxOPL03711 SCRxOPL03713 TND02844OPL10364 KTZ03391 KTZ03393 MSP02587PTR01369 SCR00709 SCR00711SCR00710SCR04238 SCR00712SCR04237 HDM01489 HDM01490HDM01491HDM01487 HDM01488ADGxSCR10639ADGxSCR10641 OPL04408BRC01118 BRC01119BRC01116 BRC01117 BRC10126BRC10129 BRC10128BRC10130BRC01123BRC01125BRC01122BRC01121 BRC01120BRC01124BRC04505BRC04455BRC04456 BRC00162 BRC00168BRC00165BRC00167 BRC00163BRC00164BRC00169 VRG03784 VRG03787VRG03788VRG03785 VRG10591VRG03786 BRC03156BRC03158BRC03157BRC03159BRC10127 SPG01903SPG01906SPG01907SPG01904BRC04485BRC04486 BRC03160OPL04487OPL04488OPL04524OPL04525 CHM03610CHM03612CHM03611 BER30107CHM03609BER00131 BER01082BER01085BER30106BER01045BER01047BER04068BER01044BER01046 BER04067BER00646BER00647 BER04066BER00649 BER00653 BER01062 BER01065BER01066BER01063 BER01064BER01083 BER01084BER00652BER04064BER04065BER00650BER00651BER04062BER04063BERxSPL01039BERxSPL01042BERxSPL01040BER01057 BER01058 BER01059BER01061BER01060 BER04061BER00648BER04070 BER30105 BER03794BER03795BER03801BER03548BER04069 BER10122 BER10125BER10121BER10124 TAR03747 BER10123BER03802BER03806BER03805BER03803BER03804 BER03808BER03809BER03807BER03810 BER03811BER01073 BER01074BER01072BER01075 BER01076 TAR03741TAR03742TAR03743TAR03745TAR03744BER00132 BER00134BER00135 BER01086BER00133BER00136 BER03789BER03790BER03792BER03791BER01048BER01051BER01049BER01050 TAR03746TAR03749 TAR03750TAR03748 BER01068 BER01069BER01071BER01067BER01070 TAR01972TAR01974TAR01973TAR01970 TAR01971TAR02030BER03793 BER00142BER04071BER00137BER00141BER00138BER00140BER00143 TAR02027TAR02033TAR02023 TAR02031 TAR02032TAR02034 TAR01961TAR02002TAR01964 TAR02754TAR02757TAR02756 TAR02758TAR02755 CHC01243 CHC03187CHC01241 CHC01242CHC00810CHC01218 CHC01219CHC01222CHC01220 CHC01221DDS02383DDS02387DDS02385 DDS02384DDS02386DDS02375DDS02376DDS02377DDS03309DDS02373DDS03312 DDS03313 DDS02374DDS03311DDS03310 ARZxHDM01001ARZxHDM03265ARZxHDM01003ARZxHDM03266 ARZxHDM03268 BER03813BER03815 BER03814BER03816BER03585 BER03586BER03587BER03584BER03588ARZxHDM00995ARZxHDM01002 ARZxHDM03267ARZxHDM03269 ARZ30101 BER01053 BER01056BER01052BER01054BER01055BER01078 BER01081BER01077BER01079BER01080BER03812CHC00806 CHC00808CHC03183CHC03186CHC00807 CHC03184CHC03185CHC00809 TAR01965TAR01969TAR01968 TAR01966TAR01967 TAR02007 TAR02008TAR02011 TAR01975 TAR10524TAR10527 TAR10525TAR10528TAR10540TAR10541TAR10539 TAR02005 TAR02009TAR02006TAR02016TAR02020TAR02022 TAR02015TAR02017 TAR02018TAR02019 TAR01976TAR01977TAR01979TAR02751TAR02753 TAR02749TAR02750TAR02752 TAR01988 TAR01989TAR01986TAR01987TAR01985 TAR01991 TAR01994TAR01992TAR01993TAR01990TAR10515 TAR10518TAR10516 TAR10517TAR10514 TAR03555TAR03557TAR03554TAR03556 BER01089BER01090 BER01091BER01088BER01087 TAR03553 TAR03369 TAR03372TAR03371TAR01963 TAR03373TAR01960TAR01962 TAR02014TAR03370TAR10535 TAR10537TAR10536TAR10538TAR10534TAR10522 TAR10532 TAR10533TAR10530TAR10526TAR10542TAR10543TAR10531 TAR10520TAR10521 TAR10523 TAR10519 TAR04255TAR04256 TAR00926TAR04253 TAR00924TAR02029 TAR00922TAR00925TAR04254TAR04252 TAR02026TAR02028TAR02025TAR00923 TAR03736TAR02021TAR02024TAR02012 TAR01978TAR02010TAR02013 TAR02001TAR02003TAR01995 TAR02004CHC00236CHC00239CHC00235CHC00243 CHC00240CHC00241CHC00234 CHC00237CHC00242 YUN02891YUN02892YUN02889YUN02888YUN02890 MLM10192 CHC02295CHC02301 HCB03347MLM10195MLM10201 TAR00627 TAR00628TAR00625 TAR00921TAR00918TAR00920TAR00624 TAR00626TAR03754 TAR04260TAR04257 TAR00917TAR30161TAR04258TAR03752TAR03753TAR00919TAR30162TAR04259TAR03755 NCD03008NCD03010 NCD03011NCD03012NCD03009 TAR03751TAR04261TAR03737TAR03739TAR03738TAR03740 TAR10511TAR10513TAR10510TAR10512TAR10509 GND00286GND00290GND00291GND00292GND00284 GND00289GND00287GND00288GND00285 GND03447 GND03448GND03450 GND03451GND03449 GND20222 GND20225GND20223 GND20224GND20221GND02960 GND02961GND02957GND02958 GND02959GND10224 GND10225GND10226 GND10222 GND10223 ALN00980ALN00982ALN00981ALN00984ALN00983 ALN03577ALN03578 ALN03574ALN03575 ALN03576 GND02468GND02471 GND02467GND02469GND02470ALN00116ALN00121 ALN04060ALN00115ALN00117ALN00119ALN00114ALN00118 ALN00120ALN02906 ALN02907ALN02904ALN02903ALN02905 DDS01291DDS01294DDS01293DDS01292DDS01295 ALN00972ALN00973 ALN00974ALN00970ALN00971DDS02378DDS02382 DDS02380DDS02381 DDS02379ALN00976 ALN00979ALN00977ALN00978ALN00975SCR01955 SCR01958SCR01956 SCR01957SCR01959 araccpapa02233araccpapa02234araccpapa02230araccpapa02231araccpapa02232CND02287CND02291CJM02286CND02288 CND02289 CND02290SCR01947 RAP02669RAP02673RAP02671RAP02670 RAP02672RAP01842RAP01845RAP01843RAP01844 RAP03945RAP03946RAP01827 RAP01828RAP01831 RAP01829RAP01830RAP10435RAP10437 RAP10436 RAP10438RAP00541RAP00542 RAP10429RAP10434 RAP10414RAP10417RAP10418RAP10416RAP10415 MED01642 RAP10425RAP30155RAP30156RAP10424RAP10426RAP10428RAP10430RAP10433 RAP10431RAP10432RAP10421RAP10419RAP10420 RAP10423RAP10422 RAP10404RAP10408RAP10405RAP10407 RAPxSPL03708RAPxSPL03709RAPxSPL03707RAPxSPL03706RAPxSPL03710 RAP03055RAP03056RAP10406 RAP01832RAP01835 RAP01834RAP01836RAP01833 RAP02665 RAP02668RAP02666 RAP02667RAP02664RAP01841 RAP01837RAP01839RAP01840RAP01838SPL03952 SPL03954SPL03955SPL03953SPL03956MCQ01687HPS10252 HPS10254HPS10251HPS10253 HPS10255 COL02346 COL02350COL02349COL02347COL02348 VRN00742 VRN00745VRN04313TAR00622SCR04243SCR04242 TAR00623SCR04246SCR00716SCR00715 SCR00717SCR04244SCR04245 SCR00713BRCxSPL03594 OKA01744 OKA01745OKA01743OKA01747OKA01738 OKA01742OKA01729 OKA01732OKA01746OKA01741 OKA01739OKA01740 OKA03943OKA03944OKA03940 OKA03941OKA03942 OKA10349OKA10351OKA10352OKA10353OKA10350 OKA10357OKA10358OKA10356OKA10354 OKA10355OKA01735OKA01737OKA01736OKA01733OKA01734OKA01731 GIG04118 GIG04119GIG04120 GIG04116GIG00667GIG00664GIG00668 GIG00665 GIG04117GIG00666 GIG01675GIG01676 GIG01673GIG01672GIG01674 GIG03889GIG03890GIG03886GIG03888GIG03887 GIG00662 GIG04121GIG00663GIG04122GIG00659 GIG00660 GIG04123GIG03881 GIG03883 GIG03885GIG04124 GIG04125GIG03882GIG00661 GIG03916 GIG03918GIG03917GIG03884GIG03919 MCD03871MCD03873 MCD03875MCD03872MCD03874GIG03925GIG03927GIG03928 GIG03926GIG03929 GIG03920GIG03922GIG03921 GIG03923GIG03924 GIG01678GIG01679GIG01677 GIG01680GIG01681MCD00387 MCD00389MCD00388 MCD00385MCD00386MCD00382 MCD00384MCD00390MCD00406MCD00407MCD00408 MCD00654MCD04115MCD04112MCD04114MCD00655MCD00656 MCD00657MCD00658 MCD01667MCD01669MCD01671MCD01670 MCD01668MCD04113 MCD03866 MCD03867MCD03869MCD03870MCD03868 GIG03911GIG03914 GIG03913 GIG03915GIG03912MCD00392 MCD00394MCD00405MCD00393 MCD00401MCD00404 MCD00402MCD00403MCD00395 MCD00391 GIG03876 GIG03877GIG03879GIG03880GIG03878 GIG03891 GIG03894GIG03895GIG03893GIG03892GIG03402GIG03404 GIG03395GIG03401GIG03403 GIG10301 GIG10302GIG10303GIG10304GIG10305 GIG01683GIG01684GIG01682 GIG01685 GIG03907GIG03909GIG03910GIG03906 GIG03908 GIG03901GIG03905GIG03902 GIG03903GIG03904GIG01686 MAG01633MAG01634MAG01631MAG01630MAG01632MCD00383 MAG03566MAG03567 MAGxMCD01627MAGxMCD01629 MAGxMCD01626MAGxMCD01628RCHxMCD10634RCHxMCD10638 RCHxMCD10635RCHxMCD10637MAG00375 MAG00381MAG00377 MAG00378MAG00376 MAG00379MAG00380 RCH02674RCH02676 RCH02678RCH02681 RCH02683RCH02680RCH02679 RCH02682MAGxMCD01625RZL01846 RZL01849RZL01847RZL01848 CHC02304 CHC02307CHC02305CHC02303CHC02306 STL02702CHC02294 ARZ02245ARZ02249ARZ02247ARZ02248CHC02293CHC02302TND02845 TND02846TND02848TND02847 CHC02311CHC02312 CHC02310CHC02308CHC02309CHC01213CHC01216CHC01214 CHC01217CHC01215 CHC10157CHC10161 CHC10158CHC10159 YUN10602 YUN10603YUN10595YUN10594 YUN10601CHC01236 CHC01239CHC01238 CHC01240CHC01234CHC01235CHC01233 CHC01232CHC03302CHC01237CHC03303CHC03301 CHC03294 CHC03295OPL?04520 OPL?04521OCR02611OCR03361OCR02610OCR02608OCR02609OCR02612 CHC10160OCR03359OCR03362 OCR03363 STL02695STL02703 STL02694STL10463STL10459 STL10462 STL10460STL10461STL02701RZL01850 YUN03573YUN10607 YUN10606YUN10609YUN10605 YUN10611 YUN10608YUN10612YUN10604 YUN10610YUN10613 ARZ00991ARZ00992ARZ00994 ARZ00990ARZ00993 ARZ20131 ARZ20135ARZ04025ARZ04026ARZ04027ARZ20132 ARZ20134ARZ20133 ARZ02238ARZ02239ARZ02235ARZ02236 ARZ02237ARZ02246 CHC01227 CHC01231 CHC01230CHC01229CHC01228 CHC00804CHC00805CHC00803 CHC00801 CHC01223CHC01224CHC01226CHQ03564CHC01225CHC01244CHC01246CHC01245TARxMCD03763TARxMCD03765TARxMCD03762 TARxMCD03761TARxMCD03764ADR00988ADR00989ADR00987 ADR00985 ADR00986MLM10193FLH03622FLH03626FLH03623 FLH03624 FLH03625CHC00238OCR03360 MTP03356MTP03358MLM10194 WBRxYUN02166WBRxYUN02167WBRxYUN02168 WBRxYUN02165WBRxYUN02169TARxARZ03756TARxARZ03758TARxARZ03759TARxARZ03757TARxARZ03760 HAN01419HAN01421HAN01418HAN01417HAN01440 HAN01438 HAN01441HAN01439HAN01437 HAN01442HAN01446HAN01443HAN01445 HAN01420HAN01425 HAN01427HAN01428HAN01422HAN01431HAN01424HAN01429HAN01423 HAN01430HAN01433HAN01436HAN01434HAN01432 HAN01435HAN01444 HAN01426VRG02877VRG02879VRG02880 VRG02876 VRG02878BAL02101 BAL02103 BAL02104BAL02095BAL02102BAL10564BAL10566 BAL10565BAL10567 VRN00747 VRN04003VRN00749 VRN00750VRN00748VRN00746 VRN04002VRN04308 VRN04306VRN04004 VRN04311VRN04312VRN03995VRN04309 BAL02093BAL02094 BAL02091BAL02090BAL02092 VRN04001 VRN00741 VRN00744VRN00752VRN04304VRN03989 VRN03988VRN03985 VRN03987 VRN04301 VRN04302VRN03986VRN04305VRN00754 VRN03981VRN03983VRN03980VRN03982VRN00751VRN00755 VRN02085VRN02086VRN02088 VRN02089 VRN02087VRN03377 VRN03378VRN03375VRN04310VRN04291VRN04292VRN04282VRN04293 VRN03374 VRN02078VRN02084VRN02082 VRN02076VRN02081VRN02080 VRN02083VRN00739 VRN00763 VRN02079VRN02075VRN02077 VRN00761VRN00762VRN00765VRN00738VRN00740 VRN00736 VRN00737 VRN03967VRN03969 VRN03968VRN04294 VRN04295 VRN03991VRN03993VRN03992 VRN03994VRN03990VRN00764VRN03376 VRN04287VRN04289VRN00760 VRN04290VRN03977VRN00757VRN00756VRN00758 VRN00759VRN04286VRN04288 VRN03978 VRN03979 VRN03975VRN03976VRN03559 VRN03561VRN03558VRN03560 VRN03971 VNT04280VRN03973VNT00933VNT00932VNT00936VNT00934VNT04279VNT00935 VRN03972VRN03974VRN03970 GRL04480SPG10481SPG10482SPG10483 SPG10479SPG10480 SPG10476SPG10478SPG10477SPG10474 SPG10475SPG03717 SPG03718SPG03716SPG03720SPG03719 SPG00571SPG00572SPG00573 SPG01910SPG01911SPG01908SPG01909SPG01912SPG02716 SPG02717SPG02714 SPG02718SPG02715SPG00576SPG00580SPG00579 SPG00581SPG00575SPG00574SPG00578SPG00577 SPG01915SPG01916SPG01914 SPG01913SPG01917 CHC02925 CHC02927CHC02928CHC02929ARZ02241 ARZ02243 ARZ02242 ARZ02244ARZ02240SPG01905 PHU00466PHU00470PHU00478PHU00479PHU00471PHU00469PHU00476 PHU00683PHU04160PHU00682 PHU00680PHU00482PHU00468 PHU02638PHU03046 PHU03048PHU03047PHU03049 CHA02321CHA02322PHU02643PHU02644PHU00472 PHU00473PHU00681 PHU01791 PHU01792PHU01790PHU02639 PHU02640PHU01788 PHU01794PHU01802PHU01801PHU01793 PHU02642CHA02319 CHA02320 CUR02354CUR02355 CUR02357CUR02356CUR02353 GON02729GON02732GON02730 GON02731GON02733 PHU00475 PHU02637PHU02645PHU02646 PHU02635PHU02636 PHU02632 PHU02634SUB02743SUB02739 STN02721 STN02723STN02725STN02719STN02722 STN02720PHU00485PHU01786 PHU00486CAN01185CAN01181 CAN01182 PHU02641SUB02741PHU00483CAN01183CAN01184 PHU04159PHU04161PHU00679PHU00474 PHU00477PHU00480PHU01783 PHU01784PHU01785 PHU01787PHU00467PHU00490PHU00481PHU00491 PHU00487PHU00489 PHU00488PHU03045PHU00484 PHU03051 PHU03052PHU03050STN00584PHU03053PHU03054 GON10494GON10495STN00583STN00585 STN00586PHU01789STN00587GON10508 GON00694 GON04208GON00695GON04209GON04210GON00693GON04207GON04211 CHA02324 CHA02326CHA02327CHA02325SUB02740SUB02742CHA02323 STN02724STN02728 STN02726STN02727GON10504 TBR02784GON10506GON10507 RH 1RH 2RH 3RH 4RH 5CHA02331 CHA02318CHA02330 PHU02633CHA02329CHA02332CHA02328 PAM03665STN00582 ADG02065 ADG02069ADG02066ADG02068 ADG02067SCR00620 AJH10113AJH10115AJH10114GON00703 GON00706GON00707 GON00701GON00705GON00704 GON10501GON10502GON10503V IO04401V IO04402 PHU01795 GON00702CHA02313 CHA02317CHA02315CHA02316CHA02314 MRN02552MRN02554MRN02553 MRN02555 MRN02556AMB02218 ORP20328 ORP20330ORP20329ORP20327ORP20326COP20182COP20185 COP20181COP20183COP20184 CHI20177CHI20178CHI20176CHI20180LMB03244LMB03245 MRN02557MRN02558MRN02560MRN02559MRN02561 AML20112AML20113AML20114AML20111 AML20115 COP10173COP10175COP10172COP10174COP10176 ORP03492ORP03495ORP03501ORP03493 ORP03494ORP03502ORP03506ORP03505ORP03504 ORP03503ORP02613ORP02616 ORP02614ORP02615 AMB10116AMB10118AMB10117AMB10120AMB10119COP20186 COP20190COP20189COP20187COP20188 VLR03539VLR03542 VLR03541VLR03543VLR03540 BUK03818 BUK03819BUK03820BUK03821BUK03817 BUK03161BUK03162 BUK03163 ABN10102 ABN10104ABN10103ABN10101COP02344 COP02345 RAP03059RAP03060RAP03057RAP03058RAP03061 AMB02221AMB02222AMB02220 AMB02223AMB02224 CND03167CND03170CND03168CND03169 CND03174CND03175CND03171CND03177CND03178CND03176 CAN03854CAN03855CAN03852 CAN03859CAN03860CAN03856CAN03857 CAN10143CAN10144CAN10142CAN10145 CAN10141CAN02918CAN02919 CHI20179 MRN01636MRN01637 MRN01639MRN01635 MRN01638 MRN02992MRN02993MRN02994 MRN03478MRN03479CAN10138 CAN10140 CAN10139CAN10136 CAN10137 COP02342 COP02343CAN03861 CAN03862CAN03865CAN03863CAN03864 AYM20141 AYM20145AYM20142 AYM20144AYM20143 VLR02070VLR02072 VLR02074VLR02071VLR02073 SPLxLPH03722SPLxLPH03724SPLxLPH03723SPLxLPH03721SPLxLPH03725MLT01702MLT01704 MLT01705MLT01703 AMB02215AMB02219PAM03663PAM03664 PAM03661PAM03662PAM03036PAM03038PAM03037 PAM03035PAM03039BUK03822 PAM03669PAM03666 BUK01131 BUK01132 BUK01135BUK01134BUK01133BUK03824BUK03825 BUK03826BUK03823 PAM03668PAM03670 PAM03667PAM10376PAM10377 PAM10375PAM10373PAM10374CAN01188 CAN01190CAN01187CAN01186CAN01189 BUK00176 BUK00178BUK00175BUK00173 BUK00174BUK00172BUK00177 MLT01720MLT01722 MLT01721MLT01723MLT01719 MLT01711MLT01712MLT01713 MLT01709MLT01710 MLT01715MLT03351MLT03352 MLT01716MLT03353 MLT01718MLT03349 BUK00170BUK00171 MLT03658MLT03659MLT03660 MLT01694 STN03075 STN03078STN03077BUK03165BUK03166BUK03164 AMY20116AMY20120AMY20119AMY20117AMY20118 AMY20122AMY20123AMY20121AMY20125AMY20124 ORP10371 ORP10372ORP10369 ORP10368ORP10370MLT10328 MLT10327MLT10326 MLT10335 MLT10337MLT10338MLT10336MLT10334 HRO20239HRO20240 HRO20237HRO20236 HRO20238 RAP10410RAP10413 RAP10412RAP10409RAP10411 TBR02790TBR02791TBR02792TBR02793TBR02789 TBR02785TBR02787TBR02788 TBR02786ADGxCUR02175ADGxCUR02179ADGxCUR02177ADGxCUR02178ADGxCUR02176 ADG02821 ADG02823ADG02817ADG02819 ADG02822ADG02820 ADG02051 ADG02052ADG02053ADG02050 ADG02054CHA02333CHA02335CHA02334 CHA02336 CND10148 TBR02794TBR02795TBR02802TBR02803TBR02801ADG02832 ADG02824ADG02828 ADG02825ADG02827ADG02826ADG02831 ADG02829ADG02830ADG02833 AJH03122AJH03123AJH03125 AJH03124AJH03126ADG02815 ADG02818ADG02814ADG02816 ADG02839ADG02842 ADG02841ADG02840 ADGxCUR10620ADGxCUR10622 ADGxCUR10623CND10146STN00692STN00690 STN00691CND10147 CND10149CND10150ADG02809ADG02811ADG02810 ADG02813ADG02812 SCR01951SCR01953 SCR01954SCR01950SCR01952 CUR02947 CUR02951CUR02949 CUR02950CUR02948VRG02163VRG02164VRG02160VRG02162VRG02161 CUR02368CUR02371CUR02369CUR02370CUR02372 ADGxCUR10619ADGxCUR10621 MGA02568 MGA02570MGA02567MGA02571 STN00689STN04202 STN04204STN04206 STN04203STN04205 STN00589STN00591 STN00590 AJH10112 SH 1SH 3SH 2SH 4SH 5ADG02834 ADG02836ADG02838ADG02835ADG02837 TBR02045TBR02046 TBR02048TBR02047TBR02049ADG02057 ADG02058ADG02055ADG02056 ADG02843TBR10544TBR10547TBR10548 TBR10545TBR10546ADG02059 ADG02804ADG02807ADG02805ADG02806 ADG02808ADGxCUR02184 ADGxCUR03261 ADGxCUR03264ADGxCUR02183ADGxCUR02180ADGxCUR03260 ADGxCUR02181 ADGxCUR02182ADGxCUR03262ADGxCUR03263 CUR02365CUR02366CUR02363 CUR02367STN00588ADG04269 CUR02359CUR02360 CUR02361CUR02358 CUR02362 WBR02171WBR02174WBR02172WBR02173WBR02170 SOU01876SOU01877CAN03858GRL04515 MGA02569MRL02582 MTP02592MTP02593 MCQ02578MCQ02579 MCQ02580MCQ02581MRL02583 MSP02590 MSP02591MSP02589MTP02595MTP02594MTP03357AMB02216 AMB02217MTP03355 AJH10111MTP03354ADG00728 ADG04270ADG00726 ADG00730ADG04271ADG04267 ADG00727ADG00729 SPL00551SPL00554ADG04268SPL00548SPL00550SPL00552 SPL00553 SPL00549 ADGxSCR10642 ADGxSCR10643ADGxSCR10640MTP02601 VRG02146 VRG02148VRG02149 VRG02145VRG02147 CND03172CND03173 LPH01604LPH01605 LPH01606LPH01602 LPH01603HMP02511 MLT01695MLT01701MLT01693MLT01692 MLT01714MLT01717MLT03350BUK00179BLB30129 BLB30128 ACL00942ACL00943ACL00945ACL00944ACL02901ACL02902ACL00946ACL00957ACL00959ACL00958 ACL00955ACL00956ACL00952ACL03104ACL03103ACL03102ACL00953ACL00954 ACL02893ACL02894ACL02895ACL00947ACL00948 TAR01980TAR01981TAR01984TAR01982TAR01983 LPH01618LPH01619LPH01617LPH01620 PNE03115PNE03116PNE03117PNE03119PNE03118PNE03113PNE03114GRL10231 ACL00101ACL00103ACL00102ACL00108ACL00105ACL00949ACL00951 ACL00950ACL00107ACL00109ACL00106PNE03108 PNE03109PNE03111PNE03112PNE03110 BUK01139 BUK01140BUK01138BUK01136 BUK01137 ACL00940ACL00941ACL00939 ACL00937ACL00938ACL03105ACL03106ACL03107 ALB20102ALB20103ALB20104ALB20105ALB20101AEM00966AEM00967AEM00965AEM00969AEM00968 ACL04055ACL04056ACL04057ACL04058ACL04059AEM00962AEM00963 AEM00961AEM00960AEM00964ACL00782ACL00784ACL00781 AEM10107AEM10108AEM10106AEM10110AEM10109IOP01581 IOP01578IOP01579IOP01580IOP01577 AEM03546 AEM03547AEM03545 JUZ02526JUZ02527JUZ02528JUZ02530JUZ02523JUZ02525JUZ02524JUZ02529JUZ02538JUZ02522JUZ02532JUZ02534JUZ02541JUZ02537JUZ02531JUZ02533JUZ02536JUZ02540JUZ02539JUZ02535 JUZ02520JUZ02521JUZ02518JUZ02519JUZ02517ALB03136ALB03140ALB03137ALB03138ALB03139 ALB03141ALB03143ALB03144ALB03142ALB03145 ALB03132ALB03133ALB03135ALB03134 DMS00264DMS00265DMS00266DMS00268MTP00415 DMS04052DMS03232DMS00273DMS00278CMM02936CMM02938CMM02937CMM02939CMM02935 DMS00270DMS00272DMS00271BUK01129 BUK01130BUK01128 DMS04050DMS04054DMS04053DMS04051DMS00267DMS00269DMS01284DMS01285DMS01282DMS01281DMS01283 DMS01287DMS01288DMS01290DMS01286DMS01289DMS10206 MTP00416MTP00418MTP00417MTP00409MTP00410MTP00411MTP00413MTP00414 DMS03229DMS03231DMS03227DMS03228DMS03230TUQ03089TUQ03090 DMS00274DMS00275DMS00276DMS00277UGT04272BLG03416SEM03072SEM03074SEM03073 DMS03233DMS03234DMS10205 BUK01126BUK01127AST02254OPL03253 EDN02405EDN02407EDN02410EDN02411EDN02414EDN02412EDN02415EDN02413EDN02416EDN02409EDN02406EDN02408EDN02402EDN02404EDN02403TUQ03092TUQ03093TUQ03091OPL03252 DMS10203 DMS10204DMS10202ACL00783 ACL00785MGA03247MGA03248MGA01647SCT10439SCT10443 SCT10441SCT10442SCT10440SCT01863SCT10445 SCT01861SCT01865SCT01864SCT01862 SCT10444SCT10446 SCT10447SCT10448SCT01851SCT01852 TOR01662TOR01666 TOR01663TOR01664TOR01665TOR01654TOR01655TOR01656 TOR01653MGA01645 MGA01646 MGA10291MGA10293MGA10294MGA10292MGA10295 TOR01649TOR01650TOR01648TOR01651TOR01652 TOR01658TOR01659 TOR01660TOR01657TOR04109 TOR04107TOR04111TOR04108TOR04110TOR00835TOR00836TOR00834TOR00837 TOR00838 TOR00833TOR04105 TOR04106TOR00832TOR04103 TOR03001TOR03002 TOR02995TOR00830TOR00831TOR00829 TOR04104 V IO03381 V IO03382V IO03383V IO03379V IO03380V IO03384 V IO10579V IO10583V IO10581 V IO10580V IO10582V IOxYUN10617V IOxYUN03778V IOxYUN10618V IOxYUN10614V IOxYUN10616V IOxYUN10615V IOxYUN03776 V IOxYUN03777 V IO02141V IO02144 V IO02142V IO02143 V IO02140 V IO10584V IO10585 V IO10587V IO10588V IO10586V IO00634 V IO00635V IO00631 V IO00632V IO00633V IO00636 V IO00637V IO00639V IO00638 LXS10262LXS10265LXS10264LXS10266LXS10263 SAN20356SAN20357 SAN20358SAN20359SAN20360SAN10455SAN10458SAN10456SAN10454SAN10457 LXS02542LXS02545 LXS02544LXS02546LXS02543BUE20151BUE20152BUE20154 BUE20155BUE20153 BUE20156BUE20159BUE20158 BUE20160BUE20157LGL10267LGL10269LGL10271LGL10268LGL10270LGL03259 MLT10331MLT10332 MLT10333MLT10329 MLT01707 MLT01708MLT03657MLT01706MLT10330 ACH02187ACH02189ACH02185 ACH02186ACH02188SCT01853SCT01854 AJH03571SCT01856SCT01859 SCT01857SCT01858 SCT30159SCT01860 MED10272MED10273 MED10275MED10274 MED01640MED01644MED01641MED10281 MED10282MED10285MED10283MED10284 MED10287MED10289MED10286 MED10278MED10279MED10277 MED10276MED10280MED10288 MED02562MED02563MED02566MED02565 MED02564MCQ02577 MCH02576MED10290 SND10450 SND10451SND10452 SND10449SND10453 WBR03095WBR03101WBR03094WBR02886 WBR02887SND03524SND03528SND03529SND03530 SND03532SND03525SND03526 SND03527SND03531 WBR02882WBR02883 WBR02884WBR02881 HCB02504 HCB02506HCB04088HCB02503HCB02505HCB03344VNT03779VNT03782VNT03781VNT03783 OKA00429 OKA00436VNT03780 OKA00677OKA04129OKA04130OKA00676OKA00678OKA00675 OKA00674NRS00419 NRS00421NRS00426 NRS00422NRS00425NRS00423 OKA00431 OKA00432OKA00433OKA00435NRS00428 NRS00420 NRS00427NRS00424NRS30144HJT30132NRS30143OKA00671 OKA30146OKA00670OKA00669 OKA04134OKA04135OKA00673OKA00672 OKA00437 OKA30145OKA00442OKA03018 OKA03019OKA00443OKA00438 OKA00446OKA00447OKA00450 OKA00444 OKA00445OKA00448OKA00449OKA00440OKA00441OKA00439 VRN03984OKA03939OKA03936OKA03937 OKA03935OKA03938 VNT02871VNT02872VNT02873VNT02875VNT02874VNT03407 VNT03408VNT03405 VNT03409VNT10561VNT10559 VNT10560VNT10562 VNT10563VNT03406 KTZ03242 KTZ03243KTZ03392KTZ03394 RCH20352KTZ03390KTZ03652KTZ03655KTZ03654KTZ03653 KTZ03650KTZ03651KTZ03649KTZ03647KTZ03648KTZ03656 KTZ01592KTZ01594KTZ01593 KTZ01595KTZ01601 KTZ02990KTZ02991KTZ02989KTZ02987KTZ02988 RCH20353 RCH20355RCH20354RCH20351KTZ02985KTZ02986 KTZ02982KTZ02983 KTZ02984 MLM02352MLM04481 MLM04527 MLM10189MLM10190MLM10187 MLM10188MLM10191MLM10182 MLM10186MLM10184MLM10183 MLM10185 MLM04495MLM04501MLM04482 CMM01279CMM01280CMM02940CMM02942CMM02941 CMM02943MLM02945MLM02946CMM02944 MLM04526CMM10179CMM10180CMM10181CMM04442CMM10177CMM10178 CMM01277CMM01278CMM03225CMM03226CMM03224 CMM04439CMM04440CMM04510 CMM04511CMM04441 CMM04459 CMM04484CMM04461CMM04462CMM04460CMM04483 CMM04443CMM04444VRN00753 BAL10568TAR10529 CHQ03562TUQ20403 SCR00708SCR00718VER04322VER04323VER04324VER04325VER00770VER02122VER04321VER02120VER02127VER02123VER02125VER02128VER02137VER02139VER02126VER02129VER02136VER30163 VER02110VER02111VER02114VER02113VER02112VER02108VER02107VER02132 VER04011VER04017VER04014VER04018VER04019VER04016 VER04010VER04013VER04012VER02105VER02106VER02109VER04015VER02133 VER04318VER04319VER04316VER04317VER04320VER00771VER02131VER02134 VER10570VER10571VER10573VER10569VER10572 SPG10485SPG10487SPG10486SPG10484VER00767VER00768VER00766HDM03627 VER02115VER02117VER02116VER02119 VER03766VER03767VER03770VER02118VER03771VER03772VER03773VER03774VER03775VER03769VER04023VER04024VER03768VER04022VER04020VER04021 MCP20295MCP20294VER02121VER02124MCP20292MCP20293MCP03473MCP03474MCP03476MCP03475MCP03477MCP20291 VER04328VER04329VER04330 VER00629VER00630VER04326VER04327 VER00779VER00780VER00778 VER00777VER10576VER10578VER10574VER10577VER10575VER02130VER02138 VER02135VER00773 VER00774 VER00769 VER00772VER00775 DMS00815 HJT01482HJT01486HJT01483HJT01485HJT01484PTA00453PTA00454PTA00452PTA00457 PTA30152PTA04154PTA30154PTA04156PTA04158PTA30153PTA04157 PTA00455PTA00456PTA00451PTA00458 PTA04147PTA04148PTA04145PTA04144PTA00462PTA00464PTA00459PTA00465PTA00463PTA00460PTA00461GRL01363 AZN03426AZN03427AZN03428AZN03425AZN03429AZN03432AZN03433AZN02463AZN02465AZN02462AZN02466AZN02464AZN20208AZN20209 AZN20210AZN20206AZN20207 AZN03431AZN03434AZN03430 FEN02452FEN02456FEN02434FEN02454FEN02455FEN02453AZN02457AZN02461AZN02460AZN02459AZN02458 CHC02932CHC02933CHC02931CHC02934CHC02930PAM03044PAM03040PAM03043PAM03042PAM03041 LPS20261LPS20265LPS20264LPS20262LPS20263FEN03330FEN03331FEN03332FEN03329FEN02435FEN02436FEN02433FEN03333 FEN02432 FEN02442FEN02444FEN02443FEN02445FEN02446 FEN02440FEN02441FEN02438FEN02439FEN02437 AZN03435AZN03436AZN03437AZN03438AZN03439 FEN02448FEN02450FEN02447FEN02451FEN02449FEN10217FEN10219FEN10218FEN10220FEN10221FEN10212FEN10216FEN10215FEN10214FEN10213 PLT30147PLT30151PLT30149 PLT30150PLT30148PLT04187PLT04188PLT04190PLT04189 PLT00526PLT00529PLT00528PLT00530PLT00531PLT00527PLT00532PLT00525 PLT00533PLT00539PLT00538PLT00535PLT00540PLT00534PLT00536PLT00537 PLT10401PLT10403PLT10402PLT03675PLT03671PLT03673PLT03672PLT03674PLT01823PLT01824PLT01825PLT01822 PLT01826PCS10383PLT00889PLT00891 STO00602STO00604STO00603STO00606STO00601STO00593STO00594STO00592STO00595 STO01931STO01934STO01933STO01930STO01932 GRL01365GRL01366GRL01367GRL01364STO03084STO03087 STO03086STO03088STO00910STN03076 STO01925STO01926STO01929 STO01928STO03085STO01927 STO01937STO01938STO00907 STO00909STO04228STO01935STO01936STO04225STO01939 STO00902STO00904STO00908STO00906STO00905 STO00903STO04229 STO04231STO04230STO00911 STO00895STO04214STO00894STO04216 STO04213STO00901STO00893STO03735STO00610 STO00608STO00613STO00615STO00612 STO00607STO00609STO00614 STO03732STO03733STO03731STO03734 STO02738STO00611RAP30157 STO02734 STO04223STO04224STO02737STO02735STO02736 STO03081STO03082STO03079STO03083STO03080 STO00913STO00914STO00915 STO00916STO00912 STO04222CHN01247 CHN01248CHN01249BLG03415 HJT00312HJT30134HJT00319HJT30133 HJT00311HJT00320HJT00314HJT00317HJT00318HJT00316HJT00315HJT00313 FEN00280FEN00281FEN00283 FEN00279FEN00282HJT01478HJT01481HJT01479HJT01480HJT01477 HJT01474HJT01476HJT01472HJT30136HJT30137HJT30135HJT03638HJT03640HJT03641HJT03639 HJT01473HJT01475HJT10246 HJT10248HJT10247HJT10250HJT10249 HJT03336HJT03337HJT03335HJT03334HJT03338MAT02602 PTA00853PTA00854PTA04146PTA00855PTA00856 PTA00849 PTA00852PTA00850PTA00857PTA00848 PLT10394PLT10395 PTA00851 PTA04149PTA04152PTA04153PTA04150PTA00860PTA00862PTA00861PTA04151RAP10427 PTA00858 STO00892 FEN01318FEN01319 FEN01316 FEN01317FEN01315 COL03217COL03221COL03219COL03220COL03222COL03216COL03223COL03218 COL02351 OTI20333OTI20334OTI20332 OTI20331OTI20335 GAB20227GAB20229GAB20228GAB20226GAB20230 AGF00111AGF00113AGF00110AGF00112AGF02195AGF02201AGF02203AGF02202 OXC03507OXC03508AGF02204 FLH20216FLH03443FLH20217FLH20220FLH20219FLH20218FLH03442FLH03444FLH03446FLH03445 FLH20214FLH20215FLH20211FLH20212FLH20213 SUP02745 SUP02746SUP02744SUP02748SUP02747AGF03120 AGF03121OXC03509OXC03511OXC03510OXC03512OXC04139OXC04140OXC04143 OXC20337OXC20338OXC20336OXC20339OXC20340OXC04141OXC00846OXC00847 OXC00844 OXC03513 ORO20324SUC20383MSP03488ORO20323 ORO20322 ORO20325ORO20321SUC20382SUC20381MSP20308MSP20310MSP20309MSP20307MSP03489MSP03491MRL03484MSP03490MSP03249 SUC20385MSP02588 MSP20306SUC20384 TND02857 TND02859TND02858TND02856 TND02855TND02850TND02860TND02849 TND02852TND02854TND02851TND02853 LGC03471LGC03472LGC03470LGC03468LGC03469LGC03246 LGC03461LGC03464LGC03463LGC03462LGC03460 LGC03387LGC03388LGC03389LGC03465LGC03466LGC03467 LGC02547LGC02549LGC02551LGC02550LGC02548LGC03385LGC03386 PRM03569 PRM03570BCP30102BCP30103BCP30104BCP00153IOP02972BCP00157IOP02976BCP00156IOP02974IOP02973BCP00161BCP00160BCP00159BCP00155IOP02975BCP00158BCP00154 BCP02914BCP02915BCP02913BCP02916BCP02917IOP30139IOP30141IOP30138 IOP30140IOP30142BCP03582BCP03583BCP03581BCP03579BCP03580 ADR00128ADR00129ADR00126ADR00127ADR00125 ADR00122ADR00123ADR00130ADR00124 GRR00302GRR00304GRR00301GRR00303GRR00306GRR00307GRR00309GRR00308GRR00310 GRR01414GRR30131GRR01416GRR01415GRR00305GRR01413 IOP01572IOP01573IOP01575IOP01576IOP01574IOP03237 IOP03241IOP03239IOP03240IOP03238BCP00790 BCP00786 SNK03069SNK03070SNK03068SNK03071SNK03066SNK03064SNK03065SNK03063SNK03067 SNK01866SNK01869SNK01867SNK01870SNK01868SNK03062 SNK02691SNK02692SNK02689SNK02690SNK02693 SNK02685SNK02686SNK02688SNK02687SNK02684HOU02964HOU02966HOU02963HOU02962HOU02967HOU02970 HOU02971HOU02968HOU02965HOU02969 HOU04085HOU04087HOU04082HOU04080HOU04081HOU04084HOU04083HOU04086HOU00824HOU00816HOU00817HOU00818HOU00819PLT00890 GRL04514CHC00802 VER00776BCP00789 CAP00207CAP00214 CAP00212CAP00206CAP00208CAP00209CAP00211CAP00210CAP00213CAP01201 CAP01202CAP01205CAP01203 CAP00215CAP00218 CAP00216CAP00217CAP01204CAP03180CAP03182 CAP03181CAP03179 CAP10151 CAP02921CAP02922CAP02920CAP02923CAP02924 CAP01192CAP01193CAP01194CAP01195CAP01191 QUM03207QUM03209QUM03206QUM03210 QUM03208CAP01206 CAP01207SST00641 QUM00255QUM00257QUM00260QUM00262QUM00258QUM00263QUM00261QUM00256 QUM00259QUM00252QUM00254QUM00253QUM01273QUM01272 QUM01274QUM10168QUM10170QUM10171 QUM10167QUM10169 CRC01262CRC01264CRC01265CRC01266CRC01263 CRC01269CRC01270CRC01267CRC01268CRC01271ABN10105 HCB00341 HCB00342HCB00343 HCB00345HCB00340HCB00336 HCB00338HCB00334HCB03348HCB00344 HCB00335HCB00339HCB00337HCB00333 HCB03345 HCB03346HCB04029HCB04031HCB20234HCB04030HCB20232 HCB04032HCB20233 HCB04028HCB20231HCB20235 SGR20377SGR20379SGR20376SGR20371SGR20374SGR20375 SGR20372SGR20373SGR02705 SGR02707SGR02706SGR02708 SGR02704SGR20380 SGR10464 SGR10467SGR10466SGR10465SGR10468 IRS20253IRS20254IRS20251IRS20255IRS20252IRS03565 IRS20259 IRS20260IRS20257 IRS20256IRS20258 PUR02657PUR02658PUR20347PUR20348 PUR20350PUR20349 PUR20346PCS03568 BLG03417 BLG03419BLG03418BLG04042 BLG04044BLG04041BLG04043BLG02255HRO20242 HRO20243HRO20244HRO20245HRO20241ACG03412ACG03414ACG03410ACG03413 ACG03544ACG03411CHQ03563 CHM01258 CHM01259CHM01252CHM01261CHM01250CHM01253CHM03605CHM03607CHM03608 CHM03604CHM03606 CHM01257 CHM01260CHM01251 CHM03194 CHM03195 CHM03193 CHM01255CHM01256CHM01254CHM04047CHM04049CHM04046CHM04045 PUR02652PUR02656CHM03203CHM03204CHM03202CHM03205CHM03201 PUR02653PUR02654 PUR02655CHM04048 PCJ20341PCJ20344PCJ20342PCJ20345PCJ20343SOL02709SOL02711SOL02712SOL02710SOL02713PCJ02627PCJ02630PCJ02629PCJ02631PCJ02628 PCJ03522PCJ03523PCJ03521PCJ10380PCJ10381PCJ10379 PCJ10378PCJ10382 TUQ10552TUQ10553TUQ10551TUQ10549TUQ10550 TUQ03538PCJ03514PCJ03520 PCJ03515PCJ03518PCJ03519PCJ03516PCJ03517 MIN20302MIN20303MIN20305MIN20301MIN20304ACP20126ACP20129ACP20127ACP20130 ACP20128SCB20362SCB20364 SCB20363 SCB20365 SCB20361BLG20149 BLG20150BLG20147 BLG20146BLG20148 MCQ10307MCQ10308 MCQ10310MCQ10309MCQ10314 MCQ10315 MCQ10311MCQ10312MCQ10313MCQ10319MCQ10316MCQ10320MCQ10317MCQ10318 MCQ04337MCQ04340MCQ04338CHN20173MCQ04339CHN20171CHN20175CHN20172 MCQ01689MCQ01691MCQ01688MCQ01690CHN20174 MCQ10306CHN10165CHN10166CHN03308CHN03306CHN10162CHN03305CHN03307CHN03304 CHN10163CHN10164 CHN03191CHN03192 CHN03188CHN03189CHN03190 HCR20249HCR20250HCR20247HCR20246HCR20248 IMT02513IMT02515IMT03457IMT02512IMT02516IMT02514IMT03455IMT03459 IMT03456IMT03458IMT03452IMT03453 IMT03454HCB02502 ABZ02206ABZ02207ABZ02213ABZ02205ABZ02209 ABZ02210ABZ02211ABZ02208ABZ02212ABZ02214ABZ03148 ABZ03146ABZ03147ABZ03150ABZ20106ABZ03149 ABZ20107ABZ20109ABZ20110ABZ20108 DCM02395 DCM02401 DCM02388DCM20202DCM20203 DCM20204DCM20205 DCM20201 DCM20191DCM20193DCM20194 DCM20192DCM20195 DCM02390DCM02392 DCM02393DCM02394DCM02391DCM03314DCM03317DCM03315DCM03318DCM03316 MSP10321MSP10324MSP10322MSP10323MSP10325 OXC00845AGU20138 AGU20140AGU20139AGU20137AGU20136ACS02191 ACS02192ACS02193 ACS02190ACS02194PLS02617PLS02618PLS02619PLS02620PLS02621PLS02623PLS02622PLS02625PLS02626PLS02624PLS01770PLS01772PLS01780PLS01782PLS01778PLS01781PLS01768PLS01769PLS01771PLS01779 PLS03020PLS03034PLS03027PLS01776PLS03021PLS03022PLS03029PLS03031PLS03032PLS03024PLS03023PLS03025PLS03026PLS03033PLS03030PLS03028PLS01777PLS01774PLS01775PLS01773 ETB10210ETB10211ETB10209ETB10207ETB03235ETB03236ETB10208ETB01314ETB01313ETB01308ETB01309ETB01310 ETB01306ETB01311ETB01307ETB01312 FRN01322FRN03441FRN01321FRN01323FRN01320 FRN01325FRN01326FRN01324FRN03440 FRN01327FRN01328FRN01329NCD04126NCD04128NCD04333 NCD04127NCD04334NCD04336 NCD00839NCD02605NCD02607NCD02603 NCD02606NCD02604 BLB01154BLB03835BLB01152BLB01151 BLB03834BLB03836BLB03833BLB01153 BLB03549BLB30119 BLB01141BLB01143BLB01144 BLB01142BLB01171BLB01174 BLB01155BLB00191BLB00194BLB00190BLB00193BLB30124BLB30126 BLB00180BLB00183BLB00181BLB30127BLB00182BLB00184BLB00185BLB00186BLB03830BLB03831 BLB30117 BLB30118BLB30116BLB30115 DPH02273DPH02274BLB00187 BLB03832BLB00188 BLB00189BLB00192 BLB01172BLB01175BLB01173BLB01158 BLB01159BLB30123BLB01160BLB01156BLB30122BLB01157BLB30125 BLB03550BLB03551BLB03552 BLB01148BLB30121BLB01147BLB01150 BLB03840BLB03841BLB04073BLB30120BLB01146 BLB01149BLB03838BLB03839BLB03828 BLB03829BLB03837BLB00792BLB00794BLB04076BLB00791 BLB01145BLB03827BLB04074 DPH20161DPH20162 DPH20164DPH20165DPH20163 DPH02271DPH02275DPH02272DPH20169 DPH20170DPH20166DPH20167DPH20168 BLB00793BLB00795 BLB04072PTT02279 PTT02280PTT02276PTT02277 BLB01178BLB01180 BLB01177BLB01162PTT02278BLB01161BLB01164 BLB30108BLB30109BLB01165BLB30113ONBEK30164 PTT02284PTT02285PTT02281PTT02282PTT02283BLB01163 BLB01170 BLB01166BLB01169BLB01168 BLB03842BLB03850BLB03848BLB03849BLB03851BLB03847BLB03843BLB03846BLB03844BLB01179BLB01176BLB03845BLB10131BLB10132BLB10135BLB10133BLB03293 BLB10134BLB03291BLB03292BLB03290BLB03289 BLB01167BLB30114BLB30112 BLB30110BLB30111CAN03853 EHR02428 EHR02429EHR03319 EHR03321EHR02427EHR02430EHR03322EHR03323EHR02431 EHR02421EHR02419 EHR03326EHR03324 EHR03327EHR02420 EHR03328EHR02418EHR03325 CPH02292 BST02257BST02258 BST02259BST03286BST03287 BST03284BST02256BST03285BST03288BST02260 CPH10152CPH10153CPH10155CPH10156CPH10154 EHR02422 EHR02423EHR02424EHR02426 BST02910BST02912BST02909BST02911BST02908EHR03320 NYR04039NYR04040 NYR20318NYR20320NYR04033NYR20311 NYR04037 BST01114BST01115BST01111BST01112BST01113 BST02269BST02270BST02268BST02266BST02267NYR20319 NYR04038NYR20315NYR04036NYR20312NYR20313 NYR04034NYR04035 NYR20317NYR20314NYR20316 BST00146BST00151BST00144 BST00147BST00145BST00152BST00148BST00149BST00150BLB04075 PNT00492PNT00502PNT00495 PNT04167PNT04173PNT00505PNT01812 PNT00509PNT01808PNT01805PNT00501 PNT01813PNT01814PNT00503PNT00494PNT00493PNT00504 PNT01815PNT00511PNT04171 PNT01804PNT01806PNT01803 PNT04163PNT04168PNT01807PNT04162 PNT00506PNT00508PNT00507PNT01816PNT00510PNT00512PNT00513 PNT01811PNT00872 PNT01809PNT01810PNT00874 PNT10385 PNT10387PNT10386PNT10388PNT10384PNT00876PNT00873 PNT00870PNT00871PNT03365PNT03366PNT03367PNT03368 PNT02648PNT02649PNT03364PNT02647PNT02651PNT02650 TRF00930 TRF00931TRF04264TRF04266 TRF00928TRF04265TRF04262 TRF00927 TRF02779TRF04263 TRF02780TRF02782 TRF02781TRF02783TRF00929 BST02262BST02263BST02265JAM10260 JAM10261JAM02977JAM02978JAM02981JAM02979JAM02980EHR02954EHR02953 EHR02955EHR02956EHR02952 JAM01585JAM01586JAM01582JAM01584 JAM01583 JAM01587JAM01590JAM01589JAM01588JAM01591JAM00348JAM00354JAM00350 JAM00349JAM00346JAM00351JAM00352 JAM00347JAM00353 BST02264 CPH00223CPH00225CPH00219CPH00221 CPH00227CPH00231CPH00228CPH00233 CPH00222 CPH00224CPH00232CPH00220CPH00226 CPH00230CPH04341 CPH01209CPH01210CPH01211CPH01208CPH01212 CPH03618CPH03619CPH03617 CPH03621CPH00229CPH03620SMB03572 BST02261TRN20388 TRN20390TRN20389TRN20387TRN02776TRN20391 TRN20392TRN20393TRN20394 TRN02765TRN02766TRN02768 TRN02774TRN02775TRN02778TRN02764 TRN02767TRN20395 TRN02777TRN02760 TRN02771TRN02772 TRN02769TRN02770TRN02773TRN02762TRN02761TRN02763TRN02759 TRN20386TRF02043TRF02044TRF02042TRF02040TRF02041 MCH03003 MCH03007MCH03004 MCH03006MCH03005 TRF02036TRF02038TRF02037TRF02039TRF02035 MCH02572MCH02573MCH02574 MCH02575 PLD01817PLD04180PLD01820PLD04186PLD00522PLD01819PLD04177PLD00517 PLD00523 PLD04183PLD00524PLD00519 PLD01821PLD00518 PLD00520PLD00886PLD00521PLD00887 PLD00884PLD00879PLD00514PLD00516PLD00515 PLD01818 PLD04182PLD04184PLD00878PLD00882 PLD10390PLD10392PLD10391PLD10389PLD10393PLD02660PLD02661PLD02663PLD02659PLD02662 LES20266LES20267LES20269LES20268LES20270PLD00881PNT00877 PLD00885PLD00880LES00368LES00369LES00371LES00370LES00373LES00372LES00366LES00367LES00374 LES20271LES20273LES20272LES20274LES20275CLR04077CLR04079 CLR03420CLR04078 CLR03613CLR03616CLR03614CLR03615CLR03421 CLR03422CLR03423CLR01275 CLR01276CLR03212 CLR03214CLR03213CLR03215CLR03211 CLR04404CLR04403 MRL03481MRL03482 MRL03480MRL03485MRL03486 MRL03487MRL02585MRL02586MRL02584 CNS00203CNS00204CNS00202 CNS00195CNS00201CNS00205FRX01332FRX01333FRX01334 FRX01330FRX01331BCP00787STO04220VRN00743 S IT01871S IT01875 S IT01872S IT01874 S IT03535S IT03537S IT03533 S IT03534S IT01873 S IT20369S IT20370S IT20367S IT20368 S IT03536S IT20366 LYC20286LYC20287LYC20282 LYC20283LYC20285LYC20279LYC20280LYC20276LYC20277 LYC20278BCP00788PNT00869CHP00246 CHP00250CHP00245CHP00247CHP00248 CHP00244CHP00251CHP00249NIG03930NIG03934NIG03933NIG03932NIG03931

0.01 changes

NJ

CbsgtotaallabelskeygeneAFLPnj

Tuberosa from Argentina and Bolivia

Tuberosa Peru and cultivated

Acaulia and Demissa

diverse, most 2n Peru

verrucosum

Polyploid Mexican/North American species and

Conicibaccata

Piurana and Circaeifolia

Mexican diploid species

Outgroup

AFLP light 916 dataset

NJ jackknife tree mp jackknife tree

AFLP light dataset: details of NJ jackknife tree

AFLP results► NJ jackknife tree shows more structure than mp jackknife tree

► 3 levels of structured groups| groups well supported in both analyses: | groups only well supported in the NJ analysis| groups recognised in NJ and mp trees but without support

► Polytomies in both trees: difficult to split up the largest group of South American species

► Causes?| This may point at a relatively recent radiation, with a certain level of

morphological divergence but less molecular divergence.

| Plus: effect is probably worsened by hybridisation.

Detailed study on South American part

►Focus on the unresolved part of the AFLP tree

►Use a population genetic approach using STRUCTURE software and Fst

►Results: not more than 16 groups instead of 90 species labels.

►Causes: misclassification, overclassification and hybridization

Use of information in the project

► Strange (AFLP) results were discussed with CGN and Keygene| Wild potato accessions were planted in the field and evaluated for expected

morphology

► Information was made accessible for users| Intranet (only accessed by CBSG members)

| Neighbour Joining tree with search options for species labels and resistance data

| Interactive module: select your own dataset: species, accession, genotypes. The built-in software will compute a dendrogram, similarity matrix or PCO or show the data on a map (based on Google Earth)

► The systematic information was used in the other part of the project and for other projects within CBSG

| Useful in choosing plant material more efficiently

Important issues

► Need for revision of taxonomy of wild potatoes

| Less species exist than species labels currently accepted

| It will take some time before a change of view will be commonly supported

► Discrepancies in taxonomic labels

| Change of species label is needed in some cases

| Important to check the morphology of the material studied

► Contrast in morphology / molecular results

| Morphological variation in wild potatoes is huge, but it cannot be captured with molecular markers AFLP nor cpDNA sequences

| Other molecular markers are needed, but do they exist?

| Managing/collecting genetic material: geographic distribution of accessions as a important alternative guideline

Molecular methods in studying biosystematics in wild...

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