Microscopes Magnification, Field of View and Estimating Size.
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Transcript of Microscopes Magnification, Field of View and Estimating Size.
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Microscopes
Magnification, Field of View and Estimating Size
![Page 2: Microscopes Magnification, Field of View and Estimating Size.](https://reader035.fdocuments.net/reader035/viewer/2022072010/56649dd55503460f94acd701/html5/thumbnails/2.jpg)
Eyepiece or Ocular Lens
Coarse Focus Knob
Fine Focus Knob
Arm
Stage Clips
Base
Revolving Nosepiece
High Power Objective Lens (40X)
Lower Power Objective Lens (10X)
Stage
Diaphragm
Light / Mirror
Body Tube
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Magnification• Total magnification is how large the specimen appears under
the microscope
Total magnification = Eyepiece (ocular) x objective lens
Objective Lens Selected
Magnification of Objective Lens
Magnification of Eyepiece (ocular)
Total Magnification
Scanning
Low Power
High Power
4X
10X
40X
10X
10X
10X
40X
100X
400X
![Page 4: Microscopes Magnification, Field of View and Estimating Size.](https://reader035.fdocuments.net/reader035/viewer/2022072010/56649dd55503460f94acd701/html5/thumbnails/4.jpg)
• How much more powerful is high power compared to the low power in terms of total magnification?
• How much more powerful is high power compared to the scanning in terms of total magnification?
![Page 5: Microscopes Magnification, Field of View and Estimating Size.](https://reader035.fdocuments.net/reader035/viewer/2022072010/56649dd55503460f94acd701/html5/thumbnails/5.jpg)
Field of View• The field of view is the white circle you see
through the microscope• As you increase magnification the field of view
decreases
![Page 6: Microscopes Magnification, Field of View and Estimating Size.](https://reader035.fdocuments.net/reader035/viewer/2022072010/56649dd55503460f94acd701/html5/thumbnails/6.jpg)
Estimating Size Under a Microscope
• The field of view will have a different diameter depending on which objective you are using
• Scanning is usually a few millimeters in diameter, low and high objective are smaller
• 1mm = 1000 micrometers (μm) or microns
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