By: Rob Page and Tara Trovarello

Light Microscope The Light microscopes are a

simple microscope that uses only one lens for magnification.

Light Microscopes can view specimens in color

An important, useful advantage over the electron microscope.

The first microscope was invented by Hans and Zacharias Janssen in 1590.

Components of a Light Microscope 1- The Ocular Lens, or also

the Eyepiece 2- Objective Turret 3- Objective Lenses 4- Coarse adjustment knob 5- Fine adjustment knob 6- Object holder, or Stage 7- Mirror, or Light 8- Diaphragm and Condenser

Different types of Light Microscopes Bright-field microscope Dark-field Microscope Phase-contrast microscope Differential-interference-

contrast microscope Fluorescence microscope Confocal microscope

Bright-Field microscope It is the simplest of all the

optical microscopy illumination techniques.

Simplicity of setup with only basic equipment required.

No sample preparation required, allows to view living cells.

Light is simply transmitted through a specimen, giving little contrast.

Staining specimens improves contrast but required that cells be fixed (not alive), which can cause distortion or alternation of components

Dark-field microscope Light is directed at an angle

toward the specimen. A condenser lens transmits

only light reflected off the specimen.

The field is dark, and the specimen is light against this dark background.

Phase-contrast microscopeComponents of the

microscope bring light waves out of phase, which produces differences in contrast and brightness when the light waves recombine.

Differential-interference contrast microscopeOut-of-phase light

waves to produce differences in contrast and brightness are combined with two beams of light traveling close together, which create even more contrast, especially at the edges of structures.

Fluorescent microscopeFluorescent stains

absorb light at one wavelength, then emit it at another.

Filters transmit only the emitted light.

Confocal microscopeLight from a laser is

focused to a point and scanned across the fluorescently stained specimen in two directions.

Clear images of one plane of the specimen are produced, while other planes of the specimen are excluded and do not blur the image.

Multiple planes can be used to reconstruct a 3-D image.

Works Citedwww.google.images www.google.com www.wikipedia.org Textbook