Martha Valdez-Moreno - Vertebrates Plenary

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The identification of Larvae, eggs and stomach contents…three studies of the application of barcodes on Mexican fish.

Transcript of Martha Valdez-Moreno - Vertebrates Plenary

  • 1. The identification of Larvae, eggs and stomach contents three studies of the applicationof barcodes on Mexican fish. Martha Elena Valdez Moreno Lourdes Vsquez Yeomans Carolina Quintal Lizama Dalia Czarez Carrillo Elva Leyva

2. ContoyPunta Allen Contoy B. ChinchorroXcalakMahahualBanco ChinchorroTheMexicanCaribbean Xcalak 3. There exist many places of reeffish spawning aggregations andnursery areas of economic andecological importance. To ensure the sustainability of these areas as integrated ecosystems and the possibility to protect them, it is essential to know 4. What fish species are living in this region? Where are they? Adults Juvenile74 mm LT22 mm LT Larvae ?19 mm LTEggs 7 mm LT 5. Applying barcodeswe have found some interesting things 6. Barcodes help us to identify the larvae69 new records for the Mexican Caribbeanonly at larvae stage Awaous banana Pterois volitans*River goby LionfishCozumel 7. 766 adult species with their larvae, previously unknown.Family GerreidaeGenus Eucinostomus E. jonesii E. melanopterusE. gulaUsing barcode The external morphology is not useful to identify these larvae.. 8. Osteology description Eucinostomus jonesiiclearing technique 9. Barcode allows us to know the distribution and thetime of the appearance of larvae species economically important8-29/march/0619/march/07 Mycteroperca bonaciBlack grouperAug/2005 Thunnus atlanticus Mar/2006 Blackfin tuna 10. 7Family GerreidaeGenus Eucinostomus E. jonesiiE. melanopterus E. gula Jan-AugAll yearMar-may Xcalak 11. Egg distribution and the time of the appearance 12. Regalecus glesneKing of herringsCozumel 9-Apr-2009 this fish could approach the coast in the north ofthe largest teleost fish of the world (8-10 m) the Mexican Caribbean for spawning or feeding 13. Lachnolaimus maximus Hogfish Xcalakadults1-30 Jan-2007 14. The case ofMycteroperca bonaciLife cycle of thespecies when and where the fishes spawn development and maintenance fisheriespropose protected areas 15. LionfishsituationinMexico 16. There are several reasons to beconcerned about their presence:They have venomous spines that couldIs the first non-injure divers.nativemarine fishestablished in the WesternNorth Atlantic andMexico has many places that areCaribbean Sea.world class scuba diving destinationLionfish are predators that consumemexican native reef species!!!What kind of prey dothey eat in the mexicancaribbean ? 17. Stomach contents What can I do? Use Barcode 18. Stomach contents analysisCozumel, Xcalak,Contoy island122 Pterois volitansBanco Chinchorro,Puerto Morelosfixed in alcohol (2009) Playa del Carmen 35 fresh digestive tracts were Mahahual placed in ethylic alcohol 96%Xcalak (2010) 90% showed a high degree of digestion of the tissueA total of 157 stomachs wereexaminedonly 144 had stomachcontentsWe separated 330 prey items 19. Results170 sequences were compared with areference library of DNA barcodes.125 sequences matched with fishes 45 sequences matched with crustacean 20. Fish: 30 different species were identified (12 families) 5 new records for Mexican Caribbean 1 surprise.Gobidae*Apogonidae* Coryphopterus eidolon C. hyalinusApogon townsendi Apogon maculatus C. venezuelae C. thrix Apogon lachneri Astrapogon puncticulatus C. tortugaeApogon mosaviPriolepis hipoliti Lythypnus sp *greatest number of species 21. Tripterygiidae Enneanectes altivelisEnneanectes boehlkei Labrisomidae mmmdelicius! Malacoctenus triangulatusStarksia ocellataLabridae Thalassoma bifasciatum Halichoeres garnotimost frecuently we foundPomacentridae Abudefduf sexatilis Stegastes partituscomelonas 22. Grammatidae Monacanthidae Gramma loreto Monacanthus tuckeriPterois volitansHaemulidae SerranidaeHaemulon flavolineatum Lioproma rubre Cephalopholis cruentata Chak-chicabrilla ScaridaeLorosSparisoma viridaeScarus iserti Sparisoma aurofrenatum species economically important 23. Crustacean45 sequencesUsing the barcodes wecould identify 20 differentspecies.3 Orders:Euphausiacea..1 speciesStomatopoda2 speciesDecapoda17 species 24. Euphausiacea Euphausia americanaEuphausia spStomatopoda Pseudosquilla sp. This only was able to assign to family level Gonodactylidae 25. Decapoda: 17 speciesHyppolytidae Thor sp. Alpheidae (CRU120) PalaemonidaeSomething like this. 26. In BoldDecapoda: 141 Familiesand 2,049 sp sequencedThe BOLD data base is notcomplete yet. 27. Conclusions1. This is an example of how important the barcodes are in the support of sustainable fisheries.2. With the help of barcodes we will know when and where the fish spawn, by directing protection measures in these particular seasons and sites we well suport fisheries.3. This is the first time we have a tool that will help us to identify all species targeted by lion fish.4. This is the first time that we have been able to report the kind of prey the lionfish consumes in the Mexican Caribbean.5. The only limit has been that the barcode library is still incomplete, mainly crustaceans, but with time it will be possible to identify all species consumed, not only by exotics, but also by any other species. 28. Acknowledgements:NOAA y RSMASCONANP Xcalak y Banco ChinchorroCONABIO y BARCODE of LIFEECOSURITCHJacc DianaManuel CaroLul SelenePaty 29. Thanks for yourattention!We need to visitthese places.Xcalak