Benchtop Linear MALDI-TOF Mass Spectrometer

MALDI-8020

MO432

The MALDI-8020 is the latest in a longline of MALDI-TOF products fromShimadzu. This benchtop, linearMALDI-TOF mass spectrometer deliversoutstanding performance in a compactfootprint, making it an ideal choice fortoday’s increasingly demandinglaboratories.

Key features:l Linear mode (positive ion) MALDI-TOF

l 200 Hz solid-state laser, 355 nm

l Load-lock chamber for fast sample introduction

l UV laser-based source cleaning (patented)

l Small footprint/benchtop design

l Quiet operation (<55 dB)

Linear mode instruments have traditionally been the

instruments of choice in MALDI-TOF-based quality control

(QC) and profiling workflows, and the MALDI-8020 is no

exception. Peptides, proteins, polymers or oligonucleotides -

the instrument is capable of delivering the performance

required for QC applications. Research laboratories will also

benefit from the MS capabilities of the instrument through

the rapid mass-measurement of samples.

Sample target solutionsCompatible with the FlexiMass™ series of microscope

slide-format sample targets, these slides provide researchers

with options depending on their experimental workflow.

The individually barcoded, single-use FlexiMass™-DS slides

provide a convenient solution for more routine or defined

workflows. Ready-to-use, these disposable targets eliminate the

need for cleaning and the risk of carryover. Alternatively, the

reusable stainless steel FlexiMass™-SR sample targets provide

a cost-effective, longer-term solution to sample preparation.

MALDI-8020: A compact footprint withoutcompromising on performance

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m/z4177 4179 4181 4183 4185 4187 4189 4191 4193 4195 4197 4199 4201

% In

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4187.26{r6343}

4188.17{r6184}

4186.20{r6700}

4189.22{r7645}

4185.14{r7693} 4190.28{r7463}

4191.34{r8757}

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800 1000 1400 1600 1800 2000 2200 2400 2800 3000 3200 3400m/z

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m/z2620 2630 2640 2650 2660 2670 2680 2690 2700 2710 2720 2730 2740 2750 2760 2770 2780 2790 2800 2810

26001200

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2651.19

2623.25 2752.17 2782.14

834.12

747.08

2094.10 1405.67

2393.26 1277.58 2250.15 1435.77

2782.14 1062.45 1536.77 3352.13 1794.87

C7

C8

C9

C10

C11 C12

C13

C14 C15

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2623.25 2522.33

C20

C21

C22

C24 C25 C26 C27

C23

H G E G T F T S D L S K Q M E E E A V R L F I E W L K N G G P S S G A P P P S

C7 C8 C9 C10 C11 C12 C13 C14 C15 C16 C17 C18 C19 C20 C21 C22 C23 C24 C25 C26 C27

Example of a 4.2 kDa peptide demonstrating an average resolutionof >5000 FWHM. Resolution, calculated by the software, is shownin brackets.

MALDI-In-Source Decay (MALDI-ISD) spectrum of native Exendin-4(top panel) showing matched fragment ions consistent with theamino acid sequence. Bottom panel: expanded region of the massspectrum showing the isotopically resolved fragment ions.

MALDI-8020

TrueClean™ automated source cleaningTo maintain instrument performance over time, the

MALDI-8020 features wide-bore ion optics - a feature used

on our existing MALDI-TOF products - which minimize the

risk of source contamination over time, providing a robust

platform.

To maximize uptime, the system is equipped with TrueClean -

an automated, rapid (<10 min) UV laser-based source

cleaning which can be used to clean the extraction electrode

in-situ without breaking instrument vacuum.

Facilitates 21 CFR Part 11 complianceOperating under the control of MALDI Solutions™ software,

the software features a centralized, secure Microsoft® SQL

database which can be used to store everything from sample

lists and acquisition Parameter Sets to acquired MALDI data.

The system is managed by an Administrator and customizable

user profiles provide control over access to the database and

operation of the instrument. Along with a full audit trail, the

software provides the tools to help achieve 21 CFR part 11

regulatory compliance.

Quality as standardAs mass spectrometry moves into clinical laboratories, the

MALDI-8020 is the perfect choice for researchers developing

MALDI-based diagnostic methods†. The system is designed

and manufactured to ISO 9001:2015 and ISO 13485:2016

quality management standards and is CE compliant.

Through the selection of components demonstrating

improved longevity and performance, and by reducing the

number of component parts, our engineers have created a

reliable and robust platform capable of delivering

outstanding performance in a small footprint.

The MALDI systems are maintained by trained service support

engineers who form part of the Shimadzu global distribution

and support network. Routine maintenance is simplified

thanks to the instrument design, providing our engineers

with easy access to commonly serviced parts.

† For Research Use Only. Not for use in diagnostic procedures.

m/z1000 1500 2500 3000 3500 4000 4500 5000 5500 6000

1111.0 1619.5

3145.4 2127.9 2636.2 1873.8

2382.0 1039.0 3399.5 1365.4 2890.3 3653.6 3144.4 1547.4

1294.4 2715.1 2055.72460.1 1801.6 3223.4

1365.1 1619.3 1110.9 1873.5

2127.6 2381.8

2635.9 2890.0

3399.2 3144.1

1951.51697.3 1443.2

2459.8 1038.7 2205.7 2713.9 3398.2 2970.1

25

127.92

823828

55.75 2464

5127.62

23818

55 245452205.7205.7

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2127.9

2382.0

2127.6 2381.8

2205.7 2309.6

254.1

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O O C

O n

Polycarbonate monomer from bisphenol A:

= 254.1

Polymer analysis: polycarbonate from a CD (green spectrum) andpolycarbonate standard (orange spectrum).Inset: expanded view of the highlighted region.

m/z

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505.2

507.3

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m/z 490 492 494 496 498 500 502 504 506 508 510 512 514 516 518 520 522 524

505.2

507.3

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Chlorhexidine

HN

Cl

NHHN NH

HN

NH

Cl

HNNHHN

NH

Analysis of chlorhexidine gluconate in mouthwash solution. Samplewas prepared with MALDI matrix without any clean-up or dilution.Inset: expanded view showing peak at 505.2 m/z.

m/z50000 100000 150000 200000 250000

80751

160349

46704

5423940812

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Example of higher mass analysis using immunoglobulin A (IgA)

Kratos Analytical Ltd.Wharfside, Trafford Wharf Road, Manchester M17 1GP, UKPhone: +44 161 888 4400 Fax. +44 161 888 4402URL http://www.shimadzu.com/an/

For Research Use Only. Not for use in diagnostic procedures.This publication may contain references to products that are not available in your country. Please contact us to check the availability ofthese products in your country.Company names, products/service names and logos used in this publication are trademarks and trade names of Shimadzu Corporation, itssubsidiaries or its affiliates, whether or not they are used with trademark symbol “TM” or “®”.Third-party trademarks and trade names may be used in this publication to refer to either the entities or their products/services, whetheror not they are used with trademark symbol “TM” or “®”.Shimadzu disclaims any proprietary interest in trademarks and trade names other than its own.

The contents of this publication are provided to you “as is” without warranty of any kind, and are subject to change without notice.Shimadzu does not assume any responsibility or liability for any damage, whether direct or indirect, relating to the use of this publication.

© Shimadzu Corporation, 2017Second Edition: November 2017, Printed in the UK MO432

MA

LDI-8020

t

The MALDI-8020 is designed and manufactured under theKratos Analytical Ltd. quality management system and is CEcompliant, CB certified and is tested to UL standards.The MALDI-8020 is a Class 1 laser product.

Shimadzu Corporationwww.shimadzu.com/an/