LQ 6XVSHQVLRQ - Cambridge University Pressunderstanding and controlling the (self-) assembly process...
2
H. Friedrich Laboratory of Materials and Interface Chemistry and Soft Matter CryoTEM Research Unit, Department of Chemical Engineering and Chemistry, Eindhoven University of Technology, P.O. Box 513 5600 MB Eindhoven, The Netherlands, [email protected]. Over the past decade electron tomography (ET) has developed into an indispensable tool for the 3D characterization of nanostructured materials [1]. The recent interest, in particular by the physical and chemical sciences, is strongly connected to advances in shaping and patterning materials on the nanoscale. Synthesis of functional nanostructures is frequently carried out in solution/suspension and understanding and controlling the (self-) assembly process are key targets for academia and industry. The ability of ET to give detailed insight into the 3D morphology of complex assemblies has considerably contributed to an understanding of their formation. For instance, ordered arrangements of nanoparticles, i.e. nanoparticle superlattices, can be formed from suspension by evaporation of the solvent. In addition to determining the local crystalline arrangement (Fig. 1), ET results indicated that superlattice nucleation and growth occurred at the suspension/air interface and that the unit cells of some lattices are anisotropically deformed upon drying [2]. For soft materials in particular, cryogenic preservation of nanostructures in their native, liquid environment is recommended as removal of the solvent leads to structural collapse [3]. An example of internally structured self- assembled nanospheres, i.e. cubosome, formed from a semicrystalline block copolymer, poly(ethylene oxide)-block-poly(octadecyl methacrylate) (PEO 45 -b-PODMA 20 ), in aqueous dispersion, is displayed in Fig. 2. Using cryo-ET it could be shown that internally bicontinuous PEO 39 -b-PODMA 17 aggregates undergo an unprecedented order-disorder transition of the micro- phase-separated domains that is accompanied by a change in the overall aggregate morphology [4]. Finally, the role of collagen in bone apatite formation was studied [5]. Combining cryo-EM with molecular modeling we show that collagen functions in synergy with inhibitors of hydroxyapatite nucleation to actively control mineralization (Fig. 3). In summary, (cryo)-electron tomography has become an indispensable tools for studying the (self-)assembly of materials in suspension [6]. References [1] H. Friedrich, P. E. de Jongh, A. J. Verkleij, K. P. de Jong, Chem. Rev. 109 (2009) 1619. [2] H. Friedrich, P.M. Frederik, G. de With, N.A.J.M. Sommerdijk Angew. Chem. Int. Ed. 49 (2010) 7850-7858. [3] H. Friedrich, C.J. Gommes, K. Overgaag, J.D. Meeldijk, W.H. Evers, B. de Nijs, M.P. Boneschanscher, P.E. de Jongh, A.J. Verkleij, K.P. de Jong, A. van Blaaderen, D. Vanmaekelbergh Nano Lett. 9 (2009) 2719. [4] B. E. McKenzie, F. Nudelman, P. H. H. Bomans, S. J. Holder, N. A. J. M. Sommerdijk J. Am. Chem. Soc. 132 (2010) 10256-10259. [5] F. Nudelman, .K. Pieterse, A. George, P. H. H. Bomans, H. Friedrich, L. J. Brylka, P. A. J. Hilbers, G. de With, N. A. J. M. Sommerdijk Nat. Mater. 9 (2010) 1004-1009. [6] HF acknowledges Prof. K. P. de Jong, Prof. D. Vanmaekelbergh, Dr. N. A. J. M. Sommerdijk, Dr. F. Nudelmann, Dr. S. J. Holder, Mr. P. H. H. Bomans, and Prof. G. de With for the fruitful 940 doi:10.1017/S1431927611005575 Microsc. Microanal. 17 (Suppl 2), 2011 © Microscopy Society of America 2011 https://doi.org/10.1017/S1431927611005575 Downloaded from https://www.cambridge.org/core. IP address: 54.39.106.173, on 22 May 2021 at 21:00:53, subject to the Cambridge Core terms of use, available at https://www.cambridge.org/core/terms.
Transcript of LQ 6XVSHQVLRQ - Cambridge University Pressunderstanding and controlling the (self-) assembly process...
H. Friedrich
Laboratory of Materials and Interface Chemistry and Soft Matter CryoTEM Research Unit, Department of Chemical Engineering and Chemistry, Eindhoven University of Technology, P.O. Box 513 5600 MB Eindhoven, The Netherlands, [email protected].
Over the past decade electron tomography (ET) has developed into an indispensable tool for the 3D characterization of nanostructured materials [1]. The recent interest, in particular by the physical and chemical sciences, is strongly connected to advances in shaping and patterning materials on the nanoscale. Synthesis of functional nanostructures is frequently carried out in solution/suspension and understanding and controlling the (self-) assembly process are key targets for academia and industry.
The ability of ET to give detailed insight into the 3D morphology of complex assemblies has considerably contributed to an understanding of their formation. For instance, ordered arrangements of nanoparticles, i.e. nanoparticle superlattices, can be formed from suspension by evaporation of the solvent. In addition to determining the local crystalline arrangement (Fig. 1), ET results indicated that superlattice nucleation and growth occurred at the suspension/air interface and that the unit cells of some lattices are anisotropically deformed upon drying [2]. For soft materials in particular, cryogenic preservation of nanostructures in their native, liquid environment is recommended as removal of the solvent leads to structural collapse [3]. An example of internally structured self-assembled nanospheres, i.e. cubosome, formed from a semicrystalline block copolymer, poly(ethylene oxide)-block-poly(octadecyl methacrylate) (PEO45-b-PODMA20), in aqueous dispersion, is displayed in Fig. 2. Using cryo-ET it could be shown that internally bicontinuous PEO39-b-PODMA17 aggregates undergo an unprecedented order-disorder transition of the micro-phase-separated domains that is accompanied by a change in the overall aggregate morphology [4]. Finally, the role of collagen in bone apatite formation was studied [5]. Combining cryo-EM with molecular modeling we show that collagen functions in synergy with inhibitors of hydroxyapatite nucleation to actively control mineralization (Fig. 3). In summary, (cryo)-electron tomography has become an indispensable tools for studying the (self-)assembly of materials in suspension [6].
References [1] H. Friedrich, P. E. de Jongh, A. J. Verkleij, K. P. de Jong, Chem. Rev. 109 (2009) 1619. [2] H. Friedrich, P.M. Frederik, G. de With, N.A.J.M. Sommerdijk Angew. Chem. Int. Ed. 49
(2010) 7850-7858. [3] H. Friedrich, C.J. Gommes, K. Overgaag, J.D. Meeldijk, W.H. Evers, B. de Nijs, M.P.
Boneschanscher, P.E. de Jongh, A.J. Verkleij, K.P. de Jong, A. van Blaaderen, D. Vanmaekelbergh Nano Lett. 9 (2009) 2719.
[4] B. E. McKenzie, F. Nudelman, P. H. H. Bomans, S. J. Holder, N. A. J. M. Sommerdijk J. Am. Chem. Soc. 132 (2010) 10256-10259.
[5] F. Nudelman, .K. Pieterse, A. George, P. H. H. Bomans, H. Friedrich, L. J. Brylka, P. A. J. Hilbers, G. de With, N. A. J. M. Sommerdijk Nat. Mater. 9 (2010) 1004-1009.
[6] HF acknowledges Prof. K. P. de Jong, Prof. D. Vanmaekelbergh, Dr. N. A. J. M. Sommerdijk, Dr. F. Nudelmann, Dr. S. J. Holder, Mr. P. H. H. Bomans, and Prof. G. de With for the fruitful
940doi:10.1017/S1431927611005575
Microsc. Microanal. 17 (Suppl 2), 2011© Microscopy Society of America 2011
https://doi.org/10.1017/S1431927611005575Downloaded from https://www.cambridge.org/core. IP address: 54.39.106.173, on 22 May 2021 at 21:00:53, subject to the Cambridge Core terms of use, available at https://www.cambridge.org/core/terms.
collaborations. This research has been supported by the Netherlands Research School Combination Catalysis and Dutch Polymer Institute (#688).
FIG. 1. Nanoparticle superlattice composed of PbSe and Au crystallites showing (a) TEM image of tilt series, (b) top view of segmented lattice, and (c) side view of segmented lattice. In the segmentation PbSe particles are shown in red and blue and Au particles in yellow.
FIG. 2. Block copolymer aggregates (PEO45-b-PODMA20) showing (a) cryo-TEM image of tilt-series, (b) numerical cross section through reconstruction, and (c) isosurface rendering of aggregates.
FIG. 3. Visualization of mineralized collagen revealing plate-shaped apatite crystals (pink) embedded in the collagen matrix.
Microsc. Microanal. 17 (Suppl 2), 2011 941
https://doi.org/10.1017/S1431927611005575Downloaded from https://www.cambridge.org/core. IP address: 54.39.106.173, on 22 May 2021 at 21:00:53, subject to the Cambridge Core terms of use, available at https://www.cambridge.org/core/terms.
![’H WDULHYHQ LQ]DNH VXFFHVVLHUHFKWHQ LQ 9ODDQGHUHQ · 431 ’H WDULHYHQ LQ]DNH VXFFHVVLHUHFKWHQ LQ 9ODDQGHUHQ Successieplanning via schenking van roerende goederen 7LP &DUQHZDO Onder](https://static.fdocuments.net/doc/165x107/6000d5776773f26f2852e206/ah-wdulhyhq-lqdnh-vxffhvvlhuhfkwhq-lq-9oddqghuhq-431-ah-wdulhyhq-lqdnh-vxffhvvlhuhfkwhq.jpg)
![3DUWLWXU KHUDXVJHJHEHQ YRQ )UDQ] 6FKHGHU …...)o $ 2e $.odu lq % $ )dj $$$ +ruq lq % >wlhi@ $ 7us lq & 3rv $$$ ` % ` 3n](https://static.fdocuments.net/doc/165x107/5e854610741e28038a721c48/3duwlwxu-khudxvjhjhehq-yrq-udq-6fkhghu-o-2e-odu-lq-dj-ruq-lq.jpg)
