Laboratory Laboratory Hardware Part Hardware Part 11

Bioscience, Equipment Unit 6Centrifuge, Water Baths, and Incubators

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BellworkBellwork

What cooking method do you use to make hard boiled eggs?

What happens to Italian salad dressing when you leave it sitting in the fridge over night?

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ObjectivesObjectives1. Identify, select, and operate

incubators2. Identify, select, and operate

water baths3. Properly load, balance, and

operate a centrifuges

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TerminologyTerminology

CentrifugeWater bathIncubator

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Water BathWater BathNo not like the tub at home for a bubble bath!

Rather a device that maintains water at a constant

temperature. It is used in the laboratory for incubations

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How to Use a Water BathHow to Use a Water BathSet the temperature of the water bath either with a dial control or a digital control.At the beginning of a lab or experiment, you should check the water bath to see if it is set at the right temperature and filled with water. Note: Water baths should be filled with distilled water. Never put your

hand in the water to test temperature

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Thumbs Up / Thumbs DownIs it important to check the temperature of the water

bath periodically while you are running an experiment

or incubation?

Be prepared to answer why

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IncubatorIncubatorA device used for growth and maintenance of microbiological cultures or cell cultures.

There are various types, sizes and uses for incubators

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Our IncubatorOur IncubatorThe incubator in our classroom is a ______________

It will control temperature ranges between ____ and _____.

It can hold approximately ____ 7cm petri dishes

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Uses for an IncubatorUses for an IncubatorDepending on the growth and type of incubator, it is possible to complete various culture grows or biological reactions while controlling temperature, degree of agitation, gas (CO2, O2, etc.) availability.

On slates:Why is an incubator a better choice

for a cell growth compared to a warm room or heat lamb? Give at

least three (3) reasons.

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Keeping Water in a Keeping Water in a GlassGlass  

Centrifuge Water Glass

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CentrifugeCentrifuge  A piece of equipment rotates tube around a fixed

axis the process causes contents to separate based on density; denser contents to the bottom of the tube lighter objects to the top.

It takes advantage of the concept of centrifugal force.

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Using a Centrifuge SafetyUsing a Centrifuge Safety-Never place flammable liquid in a centrifuge-Always use a balance to verify weight distribution-Use correct size tube-Stay with centrifuge during start up and abort run if abnormal noise or vibration- Do not open until centrifuge has completely stopped

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Using a Centrifuge SOPUsing a Centrifuge SOPLoading Samples1.Obtain correct tubes for centrifuge you will be using and check size in centrifuge before filling tubes2.Dispense samples into labeled tubes, cap securely, and insure tubes are in good condition3.If needed fill water blanks into clean tubes for balancing. Use a balance and adjust the volume in your blanks to ensure even load distribution.

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Using a Centrifuge SOP Using a Centrifuge SOP Cont. 1Cont. 1

4. Inspect the centrifuge chamber prior to use to be sure it is free of any debris, dirt or leaks. Clean if needed.

5. Load samples and blanks into opposing slots or baskets. Ensure the load is fully Balanced.

6. Close chamber lid and ensure it latches properly

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Using a Centrifuge SOP Using a Centrifuge SOP Cont. 2Cont. 2

7. Set time, temp. and speed to the desired settings prior to initiating the run.

8. Remain by centrifuge until set speed is reached. If any unusual noise or vibration occurs, stop centrifuge immediately and tell teacher.

9. Close chamber lid and ensure it latches properly

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Using a Centrifuge SOP Using a Centrifuge SOP Cont. 3Cont. 3

Retrieving samples10.When the run is complete, wait for centrifuge to come to a complete stop before attempting to open lid. 11. Clean the interior of the chamber so the centrifuge is ready for the next user.

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Identify and describe the Identify and describe the uses of the following objects uses of the following objects on your slateon your slateA

B

C

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Practice With CentrifugePractice With Centrifuge

With you lab group you will practice using the centrifuge SOP at your lab bench to separate pond water in the preloaded tubes.

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ClosureClosureIn 12 words or less summarize the

most important aspect of todays lesson.

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