LABORATORY DIAGNOSIS IN LIVER DISEASES Prof. Dr. Arzu SEVEN.
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Transcript of LABORATORY DIAGNOSIS IN LIVER DISEASES Prof. Dr. Arzu SEVEN.
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LABORATORY DIAGNOSIS IN LIVER DISEASESProf. Dr. Arzu SEVEN
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The metabolic pathways of glycolysis,the Krebs cycle,amino acid synthesis and degradation and the process of oxidative phosphorylation are all carried out in hepatocytes,which are well endowed with mitochondria.
The liver contains an extensive reticuloendothelial system for the synthesis of blood cells.
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PROTEİN SYNTHESİS İN LİVER Albumin Transthyretin(prealbumin) Igs Ceruloplasmin α1-antitrypsin Haptoglobulin β2-microglobulin Transferrin α-fetoprotein Some coagulation factors
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Albumin is the major protein product of the liver.
İt has a long biological half-life in plasma (about 20 days ) Hypoalbuminemia is a feature of advanced
chronic liver disease.
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METABOLİC LİVER DİSEASES Clinical conditions associated with
abnormal concentrations of liver_produced proteins in plasma :
1. Genetic deficiency of α1-antitripsin presents in infancy as liver disease or in adulthood as lung disease
Hepatic α1-antitrypsin belongs to the serpins, one of the family of serine protease inhibitors
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2. Genetic deficiency of ceruloplasmin leads to Wilson’s disease, a condition associated with liver and CNS damage.
Ceruloplasmin is the major cupper containing protein of liver and plasma functions as a ferrooxidase
hepatic manifestations are fulminant hepatitis + chronic hepatitis + cirrhosis
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Neuropsychiatric changes include behavioral changes + psychosis + extrapyramidal/pyramidal signs
Cupper accumulates in liver, brain,cornea, kidney and joints
Corneal rings(Kayser_Fleischer) Hemolysis + proximal renal tubular
dysfunction + osteopenia + osteoarthropathy
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Lab: Serum ceruloplasmin Urinary Cu>100 μg/d Hepatic Cu>250 μg/d AST>ALT Untreated Wilson’s disease is fatal
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Treatment: Chelation therapy with D-penicillamine(1-
2g/d)
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3. Liver cancer is associated with particularly high plasma α-fetoprotein (AFP) concentrations.
AFP and albumin have sequence homology in the fetus, AFP appears to serve physiologic
functions similiar to albumin in adult By the end of first year of life, AFP in plasma
is replaced by albumin
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4. Hemochromatosis Hereditary hemochromatosis (HHC) is an
autosomal recessive disorder of iron retention that damages liver, pancreas , heart joints, gonads and skin
Lethargy + hepatomegaly + skin pigmentation(grayish color) + sometimes DM
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Diagnosis by analysis of serum iron, ferritin and transferrin saturation
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DRUG METABOLİSM
Most drugs are metabolized by the liver. Low substrate specificity of some hepatic
enzymes produces a wide-ranging capability for drug metabolism
Hepatic metabolism usually increases the hydrophilicity of drugs and therefore their ability to be excreted.
Metabolites produced are less pharmacologically active than the substrate drug.
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However some inactive prodrugs are converted to their active forms as a result of liver processing
Metabolism proceeds in 2 phases: Phase I-addition of the polar group: The polarity of the drug is increased by
oxidation or hydroxylation catalyzed by cytocrome P-450 oxidases
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Phase II-conjugation Cytoplasmic enzymes conjugate the
functional groups introduced in the first phase by glucuronidation/sulfation/ acetylation /methylation
Cytocrome P-450 enzymes : Heme containing proteins Colocalize with NADPH:cyt P 450 reductase
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Present in endoplasmic reticulum In liver and in the epithelium of endoplasmic
reticulum There are 12 cyt P450 gene families CYP1, CYP2 and CYP3 are responsible for
most of phase I drug metabolism Induction and inhibition of cyt P-450 enzymes
is the mechanism of drug interactions.
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Cytocrome P-450 gene polymorphisms determine response to many drugs
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ALCOHOLIC LIVER DISEASE Excess intake of ethyl alcohol remains the
most common cause of liver disease in the western world.
Alcohol leads to alcoholic hepatitis, steatosis due to fat deposition/fibrosis(cirrhosis) liver failure
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Alcohol acetaldehyde acetate A B A:alcohol dehydrogenase(NAD⁺) B:aldehyde dehydrogenase(NAD⁺)
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Ethanol oxidation as a result of increased NADH/NAD⁺ alters the redox potential of hepatocyte
This inhibits oxidation of lactate to pyruvate (a step that requires NAD⁺ as a cofactor)
lactic acidosis + risk for hypoglycemia
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NADH/NAD⁺ inhibition of β-oxidation + TG synthesis
Hepatic steatosis secreted into plasma as VLDL
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Ethanol consumption affects the ubiqutin system of protein degradation, proteosome activity is decreased
hepatocyte signalling system is deregulated
apoptosis (feature of alcoholic liver disease)
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Liver function tests: Bilirubin Aminotransferases (AST and ALT ) Alkaline phosphatase (ALP ) Gamma- glutamyl transpeptidase ( γ GT ) Plasma proteins Prothrombin time
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AST-ALT Sensitive,albeit non-specific index of Acute damage to hepatocytes irrespective of
aetiology Hepatitis,toxic injury,drug overdose
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ALP İntra-or extrahepatic cholestasis Tumours Cirrhosis Liver is not the sole source of ALP activity Substantial amounts ara present in
bone,small intestine,placenta and kidney.
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In normal blood,ALP activity is derived mainly from bone and liver,with small amounts from intestine.
Placental ALP appears in maternal blood in the third trimester of pregnancy.
The liver and bone isoenzymes can be separated by electrophoresis.
Elevated γ GT suggests that the liver is
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the source of increased ALP.
γ GT
Microsomal enzyme,widely distributed in tissues including liver and renal tubules
A very sensitive index of liver pathology İncreases in cholastasis
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Alcohol and pheytoin induce enzyme activity. Prothrombin time (PT )
A measure of the activities of certain coagulation factors,made by the liver
A very short half-life İncreased PT may be the earliest indicator of
reduced hepatic synthesis.
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Acute liver disease
Acute liver damage occurs for one of the three reasons:
Poisoning İnfection İnadequate perfusion
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Chronic liver disease
Three forms of chronic liver damage are: Alcoholic fatty liver Chronic active hepatitis Primary biliary cirrhosis
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Cirrhosis is the terminal stage of chronic liver damage and only occasionally follows an acute course.
The most common causes of cirrhosis are
Chronic excess alcohol ingestion Viral hepatitis Autoimmune diseases
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