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6/97211127 NEWS ZERO

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2001

3

10-80CommitteeCau

810-80CommitteeDr.Hung

20023

5

1

Quang Tri

Ha Tinh

9

2

2003

7

3

8

10-80Committee

Dr.Xuan

2004

8

4

8

1

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2005

8

5

2006

1

1

8

6

8

26

11

2

2007

8

2

7

9

27

11

Committee33

2008

3

10-80Committee

Dr.Hung

8

28

9

8

Binh Dinh

Ha Nam

2009

2

10-80Division

Dr.Hung

5

Dr.Son

8

9

8

29

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2010

2

210-80DivisionDr.Hung

810

930

11

51

11

3

2011

1

/

5

8

11

8

31

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()

Cam Chinh Commune, Cam Lo District,Quang Tri Province

()

Cam Phuc Commune, Cam Xuyen District,Ha Tinh Province

194592

(210)33(88%)

7,877(90%=60)

80%

VND() 1=VND250

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/

8524 /7.2

(1965-71)

168-550 kg

/4311/4300( 60%)

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0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

PCDDs PCDFs PCDD/F

(pg/g

fat)

n=9)

n=9)

P

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0

2000

4000

6000

8000

10000

0.0 5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0(pgTEQ/g lipid)

(IU//37

)

N=43

R=-0.340

P=0.026

N=41

R=-0.453

P=0.003

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0

10

20

30

40

50

60

0 5 10 15 20 25 30 35 40 45(pgTEQ/g lipid)

(cm)

N=43

R= -0.373

P=0.012

N=41

R= -0.268

P=0.082

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This questionnaire was developed by Kusama et al. (2005)

as a tool to estimate the habitual nutrient intake of the

Vietnamese population

About FFQ for Vietnamese

Full-size photographs

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Standardizedcoefficients

P-value Adjusted R2

TEQ-PCDDs0.367

Area a 0.610 ***

TEQ-PCDFs

0.472

Area a 0.686 ***

Fluorine 0.257 **

Non-animal lipid 0.223 **

Zinc -0.138 0.068

TEQ-Total

0.469

Area a 0.683 ***

Fluorine 0.236 **

Non-animal lipid 0.203 **

BMI 0.177 *

Zinc -0.146 0.054

Area had the highest association with dioxin

*p

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Standardizedcoefficients P-value Adjusted R

2

TEQ-PCDDs

0.065Fluorine 0.267 *

Fiber -0.212 0.080Total FA 0.168 0.147

TEQ-PCDFs

0.119

Fluorine 0.389 **

Zinc -0.300 *

Non-animal lipid 0.247 *

BMI 0.203 0.078TEQ-Total

0.120

Fluorine 0.442 **

Zinc -0.335 *

BMI 0.221 0.056

Non-animal lipid 0.221 *

Total FA 0.199 0.080

The adjusted R2values of regression were small

*p

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Standardizedcoefficients P-value Adjusted R

2

TEQ-PCDDs

0.221

BMI 0.428 *

Ash 0.346 *

Non-animallipid 0.258 0.122

Copper -0.254 0.080

TEQ-PCDFs

0.399

Linoleic -0.726 ***

Selenium -0.518 **

Energy 0.443 **

Protein -0.435 **Animal lipid -0.404 **

BMI 0.291 *

TEQ-Total

0.212

Linoleic -0.495 **

Energy 0.466 *

BMI 0.379 *

Selenium -0.300 0.061

The adjusted R2values of regression were higher than in the sprayed areaAll models had an association between BMI and dioxins

*p

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0

0.5

1

1.5

0.86 0.68 0.48 0.31 0.17

1.06 0.84 0.58 0.38 0.21

100 25 10 5 2.5

*** p

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SCE

[]Atlas of Genetics and Cytogenetics in Oncology and Haematology http://atlasgeneticsoncology.org/Educ/Hempat_e.html

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0.0

100.0

200.0

T DD

PolyCDD

[ pg/g lipid ]

T DD

388

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0.0

0.5

1.0

1.5

2.0

2.5

3.0

3.5

4.0

TCDD

SCE value

[value/cell]

T DD

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SCE

0.0

0.1

0.2

0.3

0.4

0.5

0.6

0.0 0.5 1.0 1.5 2.0 2.5 3.0

L

o

g

S

E

Log PolyCDDs

TCDD TCDD

[ pg/g lipid ]

[ value/cell ]

y= 0.17x+0.03

r=0.62

Workshop on Agent Orange / Dioxin

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Workshop on Agent Orange / Dioxin& Health in Vietnam

[ Kanazawa, Japan ]2006.11.25 (Sat.) 9:30 16:45

Place: Division of Health Sciences, Kanazawa University

Room No. 4111Kanazawa University 10-80 Division

Corresponding to:

Teruhiko Kido

Division of Health Sciences, KANAZAWAUNIVERSITY

5-11-80 Kodatsuno, Kanazawa 920-0942Tel: 076-265-2565

Sampling PointE PS-1

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p g

( )

Sampling PointL PS-3

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3

m

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The spraying paths of

Agent Orange

The overlaid image of Landsat TM

mosaiced imageries and spraying paths of

all types of agents throughout Vietnam

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Overlaying HBT on Satellite Images(1)

These two images show the same areas

Landsat MSS image taken on Oct. 22, 1972

TNDVI Image(Transformed Normalized

Difference

Vegetation Index )

Darker area Dense vegetation

Whiter area Bare area

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Overlaying HBT on Satellite Images(3)

1989-01-24 1990-01-27

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Overlaying HBT on Satellite Images(4)

1993-02-04 Today

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Kim Bang

district,

Ha Nam province

Phu Cat

district,

Binh Dinh

province

Metabolic pathway of steroids

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OH

O

O

O

O H

OH

C O

C H 3

H O

O H

OH

O

O H

OH

C O

C H 3

O

C O

C H 3

O

O HC O

C H3

O

O HC O

C H2

O H

O

O O H

C O

C H2

O H

C H

O

OH O HC O

3

Dehydroepiandrosterone Testosterone

Estradiol

Androstenedione

Cortisol

Cortisone

Pregnenolone Progesterone

11-Deoxycortisol17-Hydroxypregnenolone 17-Hydroxyprogesterone

Cholesterol

O

OH O HC O

C H2

O H

Principle LC-MS/MS

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Principle LC MS/MS

LC

Tandem MS

Separeted steroid

By LC

Removal of the

LC solvent

1st MS

Selection of molecular

weight

Collison

cell

2ndMS

Determination of product ion

Specifity

Ionization on the LC-MS/MS

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OC

H2

F

F

FF

F

O

N+CH

3

Q1MS Q3MS

E2-PFBZPY

Precursor ion Fragmentation

(Q2MS)

Product ion

450 500 550 600 650

m/z

2.5e6

5.0e6

7.5e6

Intensity(cps)

544.3

100 200 300 400m/z

3.0e6

6.0e6

9.0e6

1.2e7

1.5e7

Intensity

(cps)

110.0

435.3339.3

181.1

313.2

m/z339

m/z181

m/z110

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F, E

T, DHT, DHEA, 4A, P4, E2

T, DHT, DHEA, 4A, P4 E2

- 1 - 2

- 3

LC-MS/MS

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y = -15.545x

2

+ 424.26x + 268.44

R = 0.1119

0

1000

2000

3000

4000

5000

6000

7000

0.00 5.00 10.00 15.00 20.00 25.00

C

s

[

p

m

l

TEQ-TOTAL [pg-TEQ/mg lipid]

Sprayed area N=16) Non-sprayed area N=9)

CortisolTEQ TOTAL

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y = -78.48x

2

+ 2217.6x + 635.27

R = 0.1839

0

5000

10000

15000

20000

25000

30000

0.00 5.00 10.00 15.00 20.00 25.00

C

s

n

[

p

m

l

TEQ-TOTAL [pg-TEQ/mg lipid]

Sprayed area N=16) Non-sprayed area N=9)

CortisoneTEQ TOTAL

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y = 0.001x

2

- 0.031x + 0.3506

R = 0.3851

0.00

0.05

0.10

0.15

0.20

0.25

0.30

0.35

0.00 5.00 10.00 15.00 20.00 25.00

E

a

o

[

p

m

l

TEQ-TOTAL [pg-TEQ/mg lipid]

Sprayed area N=16) Non-sprayed area N=6)

EstradiolTEQ TOTAL

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Salivary hormone levels in 3 years children at Phu Cat and Kim Bamg area

Exp:2011, 8,10)

Data:2011,10,10

Data check::2011.12.10

Phu Cat 52 3.87

2.85 0.61

0.52 51.12

52.18

hot spot)

NS NS

Kim Bang 59 3.34

1.92 0.62

0.76 91.96

67.16

Non-hot spot)

0.002

pg/mL

Salivary hormone levels

Area N

ng/mL ng/mL

Cortisone Cortisol DHEA

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Research Location

Major Hot

spots

Da Nang Airbase

Phu Cat Airbase

Bien Hoa Airbase

..

Table 1 Geometrical means of concentrations of PCDD/PCDF congeners

a d TEQs i b east milk f m the s f m six lati s i Viet am

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Primiparae Multiparae

TK PC ST CL CX KB TK PC ST CL CX KB

(n = 43) (n = 23) (n = 26) (n = 27) (n = 38) (n = 19) (n = 104) (n = 36) (n = 54) (n = 69) (n = 37) (n = 44)

PCDD congeners (pg/g lipid)

2,3,7,8-TetraCDD 1.97 1.66 1.39 1.05 0.63 0.50 1.47 1.09 0.98 0.75 0.47 0.311,2,3,7,8-PentaCDD 4.68 5.17 4.91 3.20 1.42 1.65 4.03 3.44 3.44 2.03 0.93 1.03

1,2,3,4,7,8-HexaCDD 2.21 2.35 2.22 1.78 0.51 0.69 2.34 1.67 2.04 1.38 0.35 0.58

1,2,3,6,7,8-HexaCDD 8.56 8.69 8.70 8.11 1.40 1.55 8.34 5.46 6.56 5.63 0.89 1.22

1,2,3,7,8,9-HexaCDD 2.70 3.18 2.67 2.18 0.47 0.71 2.67 2.21 2.21 1.54 0.30 0.51

1,2,3,4,6,7,8-HeptaCDD 11.26 14.94 10.46 15.75 1.51 2.80 13.22 11.32 10.53 12.85 1.13 2.23

OctaCDD 61.17 71.37 69.50 49.14 6.05 14.44 70.60 60.73 62.97 43.91 4.77 10.09

PCDF congeners (pg/g lipid)

2,3,7,8-TetraCDF 0.55 0.58 0.27 0.65 1.01 0.64 0.60 0.61 0.39 0.46 1.09 0.60

1,2,3,7,8-PentaCDF 1.33 1.88 0.98 0.84 0.52 0.38 1.27 1.77 1.00 0.63 0.41 0.45

2,3,4,7,8-PentaCDF 8.04 7.38 8.05 6.04 3.30 3.71 6.92 5.04 6.06 3.96 2.27 2.62

1,2,3,4,7,8-HexaCDF 18.31 16.65 18.88 17.07 2.05 2.30 17.92 11.88 13.49 11.97 0.93 1.70

1,2,3,6,7,8-HexaCDF 11.02 9.71 10.69 9.57 1.44 1.96 10.88 7.19 8.66 7.26 0.89 1.45

1,2,3,7,8,9-HexaCDF 0.33 0.36 0.20 0.33 0.09 0.14 0.31 0.28 0.15 0.28 0.08 0.12

2,3,4,6,7,8-HexaCDF 1.36 1.58 1.19 1.35 0.40 0.56 1.38 1.33 1.03 0.90 0.32 0.47

1,2,3,4,6,7,8-HeptaCDF 12.82 16.40 11.48 12.68 1.29 1.83 13.17 13.23 9.28 10.64 0.66 1.241,2,3,4,7,8,9-HeptaCDF 1.50 1.70 1.03 1.89 0.11 0.19 1.31 1.31 0.78 1.45 0.10 0.16

OctaCDF 0.63 0.99 0.49 0.19 0.11 0.26 0.61 1.00 0.52 0.25 0.12 0.29

TEQs (pg/g lipid)

PCDDs TEQ 8.33 8.50 7.93 5.78 2.34 2.59 7.21 5.75 5.86 3.92 1.62 1.66

PCDFs TEQ 5.83 5.43 5.76 4.97 1.59 1.73 5.45 3.96 4.38 3.50 1.07 1.26

PCDDs/PCDFs T EQ 14.29 14.05 13.85 10.89 3.97 4.33 12.77 9.87 10.34 7.56 2.72 2.94

Abbreviations: TK, Thanh Khe district, Da Nang city; PC, Phu Cat district, Binh Dinh province; ST, Son Tra district, Da Nang city;

CL, Cam Lo district, Quang Tri province; CX, Cam Xuyen district, Ha Tinh province; KB, Kim Bang district, Ha Nam province.

and TEQs in breast milk of mothers from six populations in VietnamPrimiparae Multiparae

TK PC ST CL CX KB TK PC ST CL CX KB

(n = 43) (n = 23) (n = 26) (n = 27) (n = 38) (n = 19) (n = 104) (n = 36) (n = 54) (n = 69) (n = 37) (n = 44)

PCDD congeners (pg/g lipid)

2,3,7,8-TetraCDD 1.97 1.66 1.39 1.05 0.63 0.50 1.47 1.09 0.98 0.75 0.47 0.311,2,3,7,8-PentaCDD 4.68 5.17 4.91 3.20 1.42 1.65 4.03 3.44 3.44 2.03 0.93 1.03

1,2,3,4,7,8-HexaCDD 2.21 2.35 2.22 1.78 0.51 0.69 2.34 1.67 2.04 1.38 0.35 0.58

1,2,3,6,7,8-HexaCDD 8.56 8.69 8.70 8.11 1.40 1.55 8.34 5.46 6.56 5.63 0.89 1.22

1,2,3,7,8,9-HexaCDD 2.70 3.18 2.67 2.18 0.47 0.71 2.67 2.21 2.21 1.54 0.30 0.51

1,2,3,4,6,7,8-HeptaCDD 11.26 14.94 10.46 15.75 1.51 2.80 13.22 11.32 10.53 12.85 1.13 2.23

OctaCDD 61.17 71.37 69.50 49.14 6.05 14.44 70.60 60.73 62.97 43.91 4.77 10.09

PCDF congeners (pg/g lipid)

2,3,7,8-TetraCDF 0.55 0.58 0.27 0.65 1.01 0.64 0.60 0.61 0.39 0.46 1.09 0.60

1,2,3,7,8-PentaCDF 1.33 1.88 0.98 0.84 0.52 0.38 1.27 1.77 1.00 0.63 0.41 0.45

2,3,4,7,8-PentaCDF 8.04 7.38 8.05 6.04 3.30 3.71 6.92 5.04 6.06 3.96 2.27 2.62

1,2,3,4,7,8-HexaCDF 18.31 16.65 18.88 17.07 2.05 2.30 17.92 11.88 13.49 11.97 0.93 1.70

1,2,3,6,7,8-HexaCDF 11.02 9.71 10.69 9.57 1.44 1.96 10.88 7.19 8.66 7.26 0.89 1.45

1,2,3,7,8,9-HexaCDF 0.33 0.36 0.20 0.33 0.09 0.14 0.31 0.28 0.15 0.28 0.08 0.12

2,3,4,6,7,8-HexaCDF 1.36 1.58 1.19 1.35 0.40 0.56 1.38 1.33 1.03 0.90 0.32 0.47

1,2,3,4,6,7,8-HeptaCDF 12.82 16.40 11.48 12.68 1.29 1.83 13.17 13.23 9.28 10.64 0.66 1.24

1,2,3,4,7,8,9-HeptaCDF 1.50 1.70 1.03 1.89 0.11 0.19 1.31 1.31 0.78 1.45 0.10 0.16

OctaCDF 0.63 0.99 0.49 0.19 0.11 0.26 0.61 1.00 0.52 0.25 0.12 0.29

TEQs (pg/g lipid)

PCDDs TEQ 8.33 8.50 7.93 5.78 2.34 2.59 7.21 5.75 5.86 3.92 1.62 1.66

PCDFs TEQ 5.83 5.43 5.76 4.97 1.59 1.73 5.45 3.96 4.38 3.50 1.07 1.26

PCDDs/PCDFs T EQ 14.29 14.05 13.85 10.89 3.97 4.33 12.77 9.87 10.34 7.56 2.72 2.94

Abbreviations: TK, Thanh Khe district, Da Nang city; PC, Phu Cat district, Binh Dinh province; ST, Son Tra district, Da Nang city;

CL, Cam Lo district, Quang Tri province; CX, Cam Xuyen district, Ha Tinh province; KB, Kim Bang district, Ha Nam province.

TK PC ST CL CX KB

(n = 43) (n = 23) (n = 26) (n = 27) (n = 38) (n = 19)

2,3,7,8-TetraCDD 1.97 1.66 1.39 1.05 0.63 0.50PCDDs/PCDFs T EQ 14.29 14.05 13.85 10.89 3.97 4.33

TK PC ST CL CX KB

(n = 104) (n = 36) (n = 54) (n = 69) (n = 37) (n = 44)

2,3,7,8-TetraCDD 1.47 1.09 0.98 0.75 0.47 0.31

PCDDs/PCDFs T EQ 12.77 9.87 10.34 7.56 2.72 2.94

Multiparae

Primiparae

Figure Daily dioxin intake of nursing infants

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105

69

27

42

60

Vietnam

Figure 3. Daily d ioxin intake of infants in Vietnam

and some other countries

JapanUnited StatesUnsprayedSprayedHot spot

100

90

80

70

60

50

40

30

20

10

0

94.4

64.5

25.4

42

60

United State : Daily dioxin intake in during the first year of life (Source: Intake of dioxins and related compounds

from food in US population. Schecter. A et al. J toxicol Environ Health A.2001 May 11; 63 (1):1-18)

Japan: Daily dioxin intake in nursing infants (Source: Report on Tolerable Daily Intake (TDI)

of Dioxins and Related Compounds. Environment Agency &Ministry of Health and Welfare 1999)

105

69

27

42

60

Vietnam

Figure 3. Daily d ioxin intake of infants in Vietnam

and some other countries

JapanUnited StatesUnsprayedSprayedHot spot

100

90

80

70

60

50

40

30

20

10

0

94.4

64.5

25.4

42

60

DDI = (volume of milk per day in ml)(fatcontent of milk/100)(PCDDs/PCDFs TEQ in breastmilk in pg/g lipid)/ (infant weight in kg).

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D

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Jinzu River

Kumano RiverIda River

(1967 to 1993) (per 1000 person)

(Kagamimori S)

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0- 5

5-10

10-2020-30

30-4040-

Prevalence of proteinuria

with glucosuria ( )

Jinzu River

Kumano

River

Ida River

Sea of Japan

Sea of Japan

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Jinzu River

Kumano River

Ida River

(1971 to 1974)

(Toyama Prefecture)

ppm

istribution of areas irrigated by

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Jinzu River

Kumano

River

Ida River

Sea of Japan

different river water

Jinzu River

Mixed

The other river

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11131 30

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11131 30

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Kakehashi

Riverbasin

Ishikawa

Jinzu River basinin Toyama

AnnakaGunma

Ichi River basin in Hyogo

Tsushima, Nagasaki

KoskaAkita

Cadmium polluted areas in Japan

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5 2

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52

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64

6

4

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22

22

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22

Fig.1 Cd(g/L)

1986

Cd5g/L

22

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Fig.2 Cd(g/g Cr)

1986

Cd5g/g Cr

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Table II. Estimated biological half-life of urinary cadmium groupedaccording to gender

Estimated biological half-life and

95%confidence intervals (years)

Urinary Cadmium (g/L)Males 10.9 (8.8, 14.4)

Females 11.5 (9.6, 14.5)

Urinary Cadmium (g/g cr)

Males 16.0 (13.0, 20.7)

Females 20.4 (16.6, 26.2)95% CI; 95% confidence interval.

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222

20082-MG

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2

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(

19

3

25

1,359 1,54711.7

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89

, ,

BMI

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BMI

9

1

1 1

65 65-74 75

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0.9

1

1.1

65 65-74 75

totally

destroyed

half destroyed

partially

destroyed

* P

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211127 NEWS ZERO

5/21/2018 Kido

94/97

Dan Duc NhuHo Dung Manh,Nguyen Hoang Viet, Pham The Tai

Nguyen Ngoc Hung, Tran Mang Hung, Phung Tri Dung, Le Thi Hong Thom

10-80

Le Ke Son

5/21/2018 Kido

95/97

13 1417COE

B14-1617-19

16 A

19-22

22-24

22 B

23-25

10-24

5/21/2018 Kido

96/97

5/21/2018 Kido

97/97