Jurnal Bioanalisis 015-036

7/18/2019 Jurnal Bioanalisis 015-036

http://slidepdf.com/reader/full/jurnal-bioanalisis-015-036 1/8

An Alternative Approach for

Quantitative Bioanalysis usingDiluted Blood to Prole OralExposure of Small Molecule

Anticancer Drugs in Mice

LOWIS YANMANIAR I22112015

STEFANIE AUDINA I22112036



• A quantitative biana!"ti#a! $et%&'( )%a($a#*ineti# +tu&ie+ u+in,&i!ute& -%!e b!& '($ +e(ia!!" b!e&$i#e -a+ &eve!)e&.



Sa$)!e )(e)a(atin

25 $i#( !ite(+ ' +$)!e &i!ute& b!& (

)!a+$a

-a+ )i)ette& int in&ivi&ua!#!u+te( tube+

10 CL ' IS +)i*in, +!utin 1000 n,$L7 5 CL ' 100 DMSO7an& 200 CL ' a#etnit(i!e -e(e a&&e& t

Sa$)!e+ -e(e t%en v(te8e& a!n, -it% t%e #a!ib(atin#u(ve +a$)!e+

t(eate& t%e +a$e -a" a+ #a!ib(atin #u(ve +a$)!e+ a+&e+#(ibe& abve



• T%i+ $et%& )(vi&e+ +i,ni9#ant )(#e++i$)(ve$ent b" en%an#in, e#ien#" '(+a$)!e #!!e#tin an& )(#e++in, an&

(e&u#in, (e+u(#e+ e.,.7 (e&u#e&#$)un&7 #+t7 an& ani$a! (equi(e$ent#$)a(e& -it% #nventina! $et%&+.

• Ou( +tu&" &e$n+t(ate+ t%e a))!i#abi!it"' u+in, &i!ute& $i#( b!& +a$)!e+ '(+$a!! $!e#u!e quantitative biana!"+i+ t+u))(t $u+e +tu&ie+ in &(u, &i+#ve(



In+t(u$en L4:MS

In+t(u$en

Da#a(ba;in7,e$#itabin7,e9nitib7i$atinib t)te#an&ibuat 1 $,$!

Die*+t(a*+i&en,an)ena$ba%an200! )(tein)(e+i)itatina+etnit(i!

Div(te8 5 $enit &i+ent(i'u,a+i15 $enit at 254.

Su)e(natan&iba,i$enGa&i 3te$)at inGe*+i", be(be&a

35! &i, untu**uanti9*a+i&a#a(ba;ine,e$#itabine

35! *uanti9*a+i,e9nitib7i$atinib t)te#an

,e9nitib7i$atinib7t)te#anPhenomenex&inetex

:B$#%.!aGu a!i( 07 $!$nit. HinGe*+iC 10 L7 -a*tuC 273$nit

10! &a(i +etia) te$)atinGe*+i &iinGe*+i*an *eL4:MS untu* &iana!i+i+

25! +a$)e!&i)i)et *e &!$

tabun, #!u+te(

)(tein)(e+i)itatin&iv(te8 &i!a*u*an +e+uai#a(a &iata+

&a#a(ba;ine 7,e$#itabineSE;# O(elisc <!aGu a!i( 07 $!$nit7 HinGe*+iC 10 L7 -a*tuC 275

$nit

#ali(ration#urve

Preparation

SamplePreparatio

n

#hromatographic

#onditions

Di!a*u*an )& &a(a%

)!a+$a &a(a%

P& DataAnalysis

Men,,una*an &ata*n+ent(a+i -a*tu(ata )!a+$a atau-a*tu in&ivu&ua!&a(a%

Jn+ent(a+i &i ba-a%bata+ *uanti+a+i&ian,,a) +eba,ai n!&a!a$ )e(%itun,anni!ai (ata:(ata untu*+etia) titi* -a*tu.



MER4I



• Met&e ini $e$be(i*an )e(bai*an )(+e+ "an,+i,ni9*an &en,an $enin,*at*an

• e9+ien+i untu* )en,u$)u!an +a$)e! &an

)en,!a%an &an $en,u(an,i +u$be( &a"a$i+a!n"a7 +en"a-a be(*u(an,7

• bia"a7 &an %e-an )e(+"a(atan &iban&in,*an&en,an $et&e *nven+ina!. Kene!itian *a$i

$enunGu**an )ene(a)an $en,,una*an +a$)e!&a(a% $i*( &ien#e(*an untu* *e#i! biana!"+i+*uantitati' $!e*u! untu* $en&u*un, +tu&i ti*u+&a!a$ )ene$uan bat

Jurnal Bioanalisis 015-036

Documents

Transcript of Jurnal Bioanalisis 015-036